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Atsushi Miyawaki's Resource

Atsushi Miyawaki's web page in English /  in Japanese.

Learn more about their research, in English  /  in Japanese (by Reseachmap[link])

List of Fluorescent protein [link] and Fucci cell cycle indicator [link]

Catalog #Name of cloneRunning titlePublication
RDB15116Venus/pCS2Venus fluorescent protein. Mammalian expression.Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. (original).
RDB15117mVenus/pRSETBVenus fluorescent protein. Bacterial expression.Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. (original).
RDB15118pNPY-Venus-N1Venus fluorescent protein targeting localization in neurites. Mammalian expression.Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. (original).
RDB15119NPY-Venus/pEF-BOSVenus fluorescent protein targeting localization in neurites. Mammalian expression.Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. (original).
RDB15120cp49VenusVenus fluorescent protein with circular permutation with the new N terminus at Thr-49.Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15121cp145VenusVenus fluorescent protein with circular permutation with the new N terminus at Tyr-145.Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15122cp157VenusVenus fluorescent protein with circular permutation with the new N terminus at Gln-157.Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15123cp173VenusVenus fluorescent protein with circular permutation with the new N terminus at Asp-173.Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15124cp195VenusVenus fluorescent protein with circular permutation with the new N terminus at Leu-195.Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15125cp229VenusVenus fluorescent protein with circular permutation with the new N terminus at Ile-229.Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15126pBS coupler 1Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. (original).
RDB15127pBS coupler 2Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. (original).
RDB15128pBS coupler 3Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. (original).
RDB15129pBS coupler 4Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. (original).
RDB15130pBS coupler 5Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. (original).
RDB15131pBS coupler 6Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. (original).
RDB15132YC2.12/pCS2Yellow cameleon calcium ion sensor clone YC2.12.Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. (original).
RDB15133YC3.12/pCS2Yellow cameleon calcium ion sensor clone YC3.12.Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15134YC2.60/pRSETBYellow cameleon calcium ion sensor clone YC2.60.Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15135YC3.60/pcDNA3Yellow cameleon calcium ion sensor clone YC3.60.Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15136YC3.60/pRSETBYellow cameleon calcium ion sensor clone YC3.60.Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15137YC3.60pm/pRSETBYellow cameleon calcium ion sensor clone YC3.60pm wiht plasma membrane localization tag.Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15138YC4.60/pRSETBYellow cameleon calcium ion sensor clone YC4.60.Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15139Flash pericam/pcDNA3Calcium ion sensor clone Flash pericam having a circularly permuted YFP and a CaM.Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15140Inverse pericam/pcDNA3Calcium ion sensor clone Inverse pericam having a circularly permuted YFP and a CaM.Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15141Split pericam-N/pcDNA3N-terminal half portion of Pericam.Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15142Split pericam-C/pcDNA3C-terminal half portion of Pericam.Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15143Ratiometric pericam/pcDNA3Calcium ion sensor clone Ratiometric pericam having a circularly permuted YFP and a CaM.Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15144Ratiometric pericam-mt/pcDNA3Calcium ion sensor clone Ratiometric pericam-mt tagged with mitchondria localization.Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15145Ratiometric pericam-nu/pcDNA3Calcium ion sensor clone Ratiometric pericam-nu tagged with nuclear localization signal.Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15229hmAG407/pRSETBPlasmid clone of fluorescent protein monomeric Azami-Green (mAG).Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. (original).
RDB15230hmAG1/pRSETBPlasmid clone of fluorescent protein monomeric Azami-Green (mAG).Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. (original).
RDB15231Kaede/pRSETBPlasmid clone of fluorescent protein Kaede which shows UV-induced green-to-red photoconversion.Ando, R., An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656 (2002). PMID 12271129. (original).
RDB15232tdKeima/pRSETBPlasmid clone of tdKeima.Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. (original).
RDB15233mKeima/pcDNA3Plasmid clone of monomeric Keima-Red (mKeima).Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. (original).
RDB15234dKeima/pRSETBPlasmd clone of dimeric Keima-Red (dKeima).Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. (original).
RDB15235dKeima570/pRSETBKogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. (original).
RDB15236mKeima (h)/pCS2Expression vector of monomeric Keima-Red (mKeima).Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. (original).
RDB15237dKeima (h)/pCS2Expression vector of dimeric Keima-Red (dKeima).Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. (original).
RDB15238mt-mKeima (h)/pIND(SP1)Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. (original).
RDB15240MiCy-DEVD-mKO1/pCS2Expression vector of FRET based caspase3 activity monitoring probe.Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. (original).
RDB15241mMiCy1/pRSETBPlasmid clone of fluorescent protein monomeric Midoriishi-Cyan (mMiCy1).Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. (original).
RDB15242cjBlue/pRSETBPlasmid clone of cjBlue., a blue color protein of sea anemone Cnidopus japonicus.Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187. (original).
RDB15243cjBlue Y64L/pRSETBPlasmid clone of yellow color protein, a variant of cjBlue of sea anemone Cnidopus japonicus.Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187. (original).
RDB15244mK-GOProtein-based fluorescent timer, monomeric Kusabira Green Orange (mK-GO).Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833. (original).
RDB15245NPY-mK-GO/pcDNA3.1(+)Neuropeptide Y (NPY)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO).Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833. (original).
RDB15246tPA-mK-GO-N1Tissue-type plasminogen activator (tPA)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO).Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833. (original).
RDB15247Flamindo/pcDNA3Expression vector of yellow fluorescent biosensor for cAMP, Flamindo. Kitaguchi, T., Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells. Biochem. J. 450 (2): 365-373 (2013). PMID 23282092. (original).
RDB15248Flamindo (R279E)/pcDNA3Expression vector of mutant Flamindo (negative control).Kitaguchi, T., Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells. Biochem. J. 450 (2): 365-373 (2013). PMID 23282092. (original).
RDB15249GEPRA-BGenetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-beta.Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. (original).
RDB15250GEPRA-GGenetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-gamma.Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. (original).
RDB15251GEPRA-AAGenetically encoded indicator for RA, GEPRA. Low-affinity verson of the GEPRA-B.Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. (original).
RDB15252GEPRA-B/ pT2KXIGdeltainExpression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish.Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. (original).
RDB15253GEPRA-G/ pT2KXIGdeltainExpression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish.Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. (original).
RDB15254GEPRA-AA/ pT2KXIGdeltainExpression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish. Low-affinity verson of the GEPRA-B.Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. (original).
RDB1525522G/pRSETBAndo, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. (original).
RDB15256Dronpa/pRSETBPhotoswitchable fluorescent protein Dronpa.Ando, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. (original).
RDB15257Dronpa2/pRSETBPlasmid clone of Dronpa2.Ando, R., Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99 (2007). PMID 17384059. (original).
RDB15258Dronpa3/pRSETBAndo, R., Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99 (2007). PMID 17384059. (original).
RDB15259KikGR/pRSETBPhotoswitchable green fluorescent protein, Kikume Green-Red (KikGR).Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. (original).
RDB15261mKikGR/pRSETBPhotoswitchable green fluorescent protein, monomeric Kikume Green-Red (mKikGR).Habuchi, S., mKikGR, a monomeric photoswitchable fluorescent protein. PLoS One 3 (12): e3944 (2008). PMID 19079591. (original).
RDB15262MermaidVoltage indicator Mermaid.Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. (original).
RDB15263Mermaid2Voltage probe Mermaid2.Tsutsui, H., Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. J. Physiol. 591 (18): 4427-4437 (2013). PMID 23836686. (original).
RDB15264XpaPlasmid clone of a photoswitchable variant of KikGR.Tsutsui, H., A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193 (2015). PMID 25773597. (original).
RDB15265Xpa H62QPlasmid clone of a non-photoconvertivle variant of KikGR.Tsutsui, H., A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193 (2015). PMID 25773597. (original).
RDB15267mKO2-hCdt1(30/120) / pCSII-EFLentivirus vector plasmid of Fucci-G1 phase probe. Component of Fucci cell cycle probe.Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078. (original).
RDB15268mAG-hGeminin(1/110) / pCSII-EFLentivirus vector plasmid of mAG-hGeminin(1/110). Component of Fucci cell cycle probe.Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078. (original).
RDB15269mCherry-hGeminin(1/110) / pCSII-EFLentivirus vector plasmid of S/G2/M-R(N).Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. (original).
RDB15270mCherry-hGeminin(1/60) / pCSII-EFLentivirus vector plasmid of S/G2/M-R(NC).Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. (original).
RDB15271mVenus-hGeminin(1/110) / pCSII-EFLentivirus vector plasmid of Fucci2-S/G2/M phase probe. Component of Fucci2 cell cycle probe.Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. (original).
RDB15272mVenus-hGeminin(1/60) / pCSII-EFLentivirus vector plasmid of S/G2/M-Y(NC).Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. (original).
RDB15273mCherry-hCdt1(30/120)/ pCSII-EFLentivirus vector plasmid of Fucci2-G1 phase probe. Component of Fucci2 cell cycle probe.Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. (original).
RDB15274AmCyan-hGem(1/110)/pCSII-EFLentivirus vector plasmid of AmCyan-hGeminin(1/110). EF-1 alpha promoter.Nishimura, K., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789. (original).
RDB15275mKO2-zCdt1(1/190) / pT2KXIGdeltainzCdt1-based G1 marker for zebrafish cells to label nuclei with orange fluorescent protein mKO2. A component of the zFucci.Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. (original).
RDB15276mAG-zGeminin(1/100) / pT2KXIGdeltainzGem-based S/G2/M marker for zebrafish cells to label nuclei with green fluorescent protein mAG. A component of the zFucci.Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. (original).
RDB15277mAG-hGeminin (1/60) / pT2KXIGdeltainhGem-based S/G2/M marker. for zebrafish cells to label nuclei with green fluorescent protein mAG.Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. (original).
RDB15278mCherry-zCdt1(1/190) / pT2KXIGdeltainzCdt1-based G1 marker for zebrafish cells to label nuclei with red fluorescent protein mCherry.Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. (original).
RDB15279mVenus-zGeminin (1/100) / pT2KXIGdeltainzGem-based S/G2/M marker for zebrafish cells to label nuclei with yellow fluorescent protein mVenus.Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. (original).
RDB15312hmKO1/pRSETBPlasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO1).Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. (original).
RDB15313hmKO2/pRSETBPlasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO2). A fast-folding variant of mKO.Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. (original).
RDB15314hmKO-K/pRSETBPlasmid clone of hmKO-K, faster-maturating version of Kusabira orange.Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. (original).
RDB15441mCherry-hCdt1(1/100)Cy(-)/pCSII-EFLentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-). EF-1 alpha promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15442mCherry-hCdt1(1/100)/pCSII-EFLentivirus vector plasmid of mCherry-hCdt1(1/100). EF-1 alpha promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15443tFucci(SA)2/pCSII-EFLentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15444tFucci(SA)2.1/pCSII-EFLentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15445tFucci(SA)2.2/pCSII-EFLentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). EF-1 alpha promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15446tFucci(CA)2/pCSII-EFLentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15447tFucci(CA)2.1/pCSII-EFLentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15448tFucci(CA)2.2/pCSII-EFLentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). EF-1 alpha promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15449tFucci(SA)2/pCSII-CMVLentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). CMV promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15450tFucci(SA)2.1/pCSII-CMVLentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). CMV promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15451tFucci(SA)2.2/pCSII-CMVLentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). CMV promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15452tFucci(CA)2/pCSII-CMVLentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mVenus-hGem(1/110). CMV promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15453tFucci(CA)2.1/pCSII-CMVLentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_AmCyan-hGem(1/110). CMV promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15454tFucci(CA)2.2/pCSII-CMVLentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). CMV promoter.Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15455YCX2.60/pCS2Yellow cameleon calcium ion sensor clone YCX2.60.Madisen, L., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron 85 (5): 942-958 (2015). PMID 25741722. (original).
RDB15456mt-mKeima/pcDNA3Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. (original).
RDB15457mCherry-hCdt1(30/120)/pcDNA3Expression vector of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe.Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. (original).
RDB15458mVenus-hGeminin(1/110)/pcDNA3Expression vector of Fucci2-S/G2/M phase probe. Component of Fucci2 cell cycle probe.Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. (original).
RDB15459mCherry-hCdt1(1/100)Cy(-)/pcDNA3Expression vector of mCherry-hCdt1(1/100)Cy(-).Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. (original).
RDB15469pET28/Dronpa-theta-D4Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens.Mizuno, H., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011). (original).
RDB15470pET28/Dronpa-NT-LysMarker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.Mizuno, H., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011). (original).
RDB15703UnaG/pcDNA3-FLAGExpression vector of eel UnaG, bilirubin-inducible fluorescent protein.Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684. (original).
RDB15704UnaG/pGEX-2TExpression vector of eel UnaG, bilirubin-inducible fluorescent protein.Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684. (original).
RDB15705SCAT3.1/pcDNA4 HMBExpression vector of FRET based apoptosis probe.Nagai, T., A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PMID 15158444. (original).
RDB15706Cy11.5 /pRSET BChimeric fluorescent protein. CFP-YFP fusion protein of concatenation form.Shimozono, S., Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Biochemistry 45 (20): 6267-6271 (2006). PMID 16700538. (original).
RDB15780ptol2/cmlc2-mermaidTsutsui, H., Visualizing voltage dynamics in zebrafish heart. J. Physiol. 588 (Pt 12): 2017-2021 (2010). PMID 20421282. (original).
RDB15781pcDNA3 Venus-AkalucExpression vector of Akaluc luciferase.Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486. (original).
RDB15782pAAV2 SynTetOff Venus-AkalucExpression vector of Akaluc luciferase.Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486. (original).
RDB15783pAAV2 TRE Venus-AkalucExpression vector of Akaluc luciferase.Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486. (original).
RDB15908pRSETB-BReleaCaBacteria expression vector of UnaG/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.Shitashima, Y., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PMID 29395087. (original).
RDB15909pcDNA3-BReleaCaExpression vector of UnaG/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.Shitashima, Y., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PMID 29395087. (original).
RDB15982Achilles/pRSETBYellow fluorescent protein clone Achilles.Yoshioka-Kobayashi, K., Coupling delay controls synchronized oscillation in the segmentation clock. Nature. 2020 Jan 8. doi: 10.1038/s41586-019-1882-z. [Epub ahead of print] PMID 31915376. (original).
RDB15994pdsVenusDestabilized Venus (dsVenus) expression vector with CMV promoter.Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. (original).
RDB18218AzaleaB5/pRSETbNew coral derived red-emitting fluorescent protein AzaleaB5.Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156. (original).
RDB18219h2-3/pRSETbNew coral derived green-emitting fluorescent protein h2-3.Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156. (original).
RDB18220tFucci(SA)5/pCS2New color variant of Fucci: Fucci(SA)5.Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156. (original).
RDB18221tFucci(CA)5/pCS2New color variant of Fucci: Fucci(CA)5.Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156. (original).
RDB18222SRAI_pcDNA3Expression vector of fluorescent protein, SRAI (signal-retaining autophagy indicator) for autophagy sensor.Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. (original).
RDB18223mito-SRAI_pcDNA3Expression vector of fluorescent protein, mito-SRAI (signal-retaining autophagy indicator) for quantitative visualizing mitophagy.Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. (original).
RDB18684pAAV2-TRE-mito-SRAI-BGHpAAAV2 expression vector of mito-SRAI (signal-retaining autophagy indicator) driven by the tetracyclin-responsive element promoter.Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. (original).
RDB18685pAAV2-SYN-tTAad-BGHpAAAV2 expression vector of tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter, human synapsin I.Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. (original).

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2021.03.23

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