Please review the QC test results indicated by icon below as well as clone information before placing your order.

(n1)StayGold(c4)/pRSET

Fluorescent protein StayGold for fusion to the N-terminus of target molecule

Sequence (full)	RDB19606zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19606_B2Eap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724) Region: 6xHis,T7 tag,Xpress tag,insert 5' Sequence file: RDB19606_B2Eaa.seq
>D08836A2_B2Ea_3_lacIpro-B_F_E05_14_ABI24.ab1     1 NNNNNNNNNN NNNNNCGNNN CGNGTCNGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC    61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT   121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC   181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA   241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGA   301 TGGTGTCTAC AGGCGAGGAG CTGTTTACCG GCGTGGTGCC CTTCAAGTTC CAGCTGAAGG   361 GCACCATCAA CGGCAAGAGC TTCACCGTGG AAGGCGAGGG CGAGGGCAAT AGCCACGAGG   421 GCAGCCACAA AGGCAAGTAC GTGTGCACCA GCGGCAAACT GCCAATGTCT TGGGCCGCCC   481 TGGGAACTAG CTTCGGCTAT GGCATGAAGT ACTACACCAA GTACCCCAGC GGCCTGAAGA   541 ACTGGTTCCA CGAGGTGATG CCCGAGGGCT TCACCTACGA CAGACACATC CAGTACAAGG   601 GCGACGGCAG CATCCACGCC AAGCACCAGC ACTTCATGAA GAACGGCACC TACCACAACA   661 TCGTGGAGTT CACCGGCCAG GACTTCAAGG AGAACAGCCC CGTGCTGACC GGCGACATGA   721 ACGTGAGCCT GCCCAACGAG GTGCAGCACA TCCCCAGAGA TGACGGCGTG GAGTGNCCAG   781 TGACCCTGCT GTACCCTCTG CTGAGCGACA AGAGCAAGTG CGTGGANGCC CACCAGAACA   841 CCATCTGCNA GCCCCTGCAC AATCAGCCAG CCCCCGATGT GNCNTACCAC NNGATCAGAA   901 AGCAGTACAC CCAGANCAAG GNNGANNCCN NNGANAGAGA NCACATCTGN CANANCGAGN   961 NCCNGNANNN CNNNCNGNCN NNNNNCNANC CTTCNNNNNC NGNCGNGNNA NANNTNNNNN 1021 NNNNN //
Primer: f1ori_R3 (Pr0142) Region: T7 ter,insert 3' Sequence file: RDB19606_B2Eab.seq
>D08836A2_B2Ea_3_f1ori_R3_F05_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNA NNGTTGGGAN GGCGATCGGT GCGGGCCTCT TCGCTATTAC    61 GCCAGATCCG GATATAGTTC CTCCTTTCAG CAAAAAACCC CTCAAGACCC GTTTAGAGGC   121 CCCAAGGGGT TATGCTAGTT ATTGCTCAGC GGTGGCAGCA GCCAACTCAG CTTCCTTTCG   181 GGCTTTGTTA GCAGCCGGAT CAAGCTTCGA ATTCTTACAC GGCAGAAGCA GAAGGCTCGT   241 GCCATGGCAG GTGGGCCTCC AGGGTCTCGC TCTGGCAGAT GTGGTCTCTC TCCTCGGTGT   301 CGTCCTTGCT CTGGGTGTAC TGCTTTCTGA TCCAGTGGTA TGGCACATCG GGGGCTGGCT   361 GATTGTGCAG GGGCTTGCAG ATGGTGTTCT GGTGGGCCTC CACGCACTTG CTCTTGTCGC   421 TCAGCAGAGG GTACAGCAGG GTCACTGGGC ACTCCACGCC GTCATCTCTG GGGATGTGCT   481 GCACCTCGTT GGGCAGGCTC ACGTTCATGT CGCCGGTCAG CACGGGGCTG TTCTCCTTGA   541 AGTCCTGGCC GGTGAACTCC ACGATGTTGT GGTAGGTGCC GTTCTTCATG AAGTGCTGGT   601 GCTTGGCGTG GATGCTGCCG TCGCCCTTGT ACTGGATGTG TCTGTCGTAG GTGAAGCCCT   661 CGGGCATCAC CTCGTGGAAC CAGTTCTTCA GGCCGCTGGG GTACTTGGTG TAGTACTTCA   721 TGCCATAGCC GAAGCTAGTT CCCAGGGCGG CCCAAGACAT TGGCAGTTTG CCGCTGGTGC   781 ACACGTACTT GCCTTTGTGG CTGCCCTCGT GGCTATTGNC CTCGCCCTCG CCTTCCACGG   841 TGAAGCTCTT GCCGTTGANG GTGCCNTTCA GCTGGAACTT GAANGGCACC ACGCCGGTAA   901 ACAGCTCCTC GCCNGTAGAN NCCNTCGGAT NCTTATCGTC ATCGNCNNAC AGNTNCNNAC   961 CNNTTTGCTG NNCNCCAGTC NNGCTANCCN TANNNNGNTG ANGNNGATGA NGAGANNNNN 1021 NNNNATGTAT ATCNNCNNNN NNNANGNTNA ANNAANTNNN TNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl