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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

(n1)StayGold(c4)/pRSET

Fluorescent protein StayGold for fusion to the N-terminus of target molecule

Catalog number RDB19606
Resource name (n1)StayGold(c4)/pRSET
Clone info. For fusion of StayGold to the N-terminus of a target molecule (X). c4 is the appendage that facilitates fusions to the C-terminus of StayGold. The gene for StayGold in this construct has been resynthesized with mammalian-preferred codons. See Hirano, M. et al., Nat. Biotechnol. 2022 doi: 10.1038/s41587-022-01278-2 for more information.
Comment The accession numbers in the DDBJ/EMBL/GenBank databases are LC601652 for StayGold, LC593679 for h-StayGold and LC601653 for oxStayGold.
Vector backbone pRSET (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Ampicillin
Gene/insert name Cytaeis uchidae GFP cDNA
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |
Other clones in our bank

External Database
Cytaeis uchidae GFP

            Reference sequence
              
             
            Sequence (full) RDB19606zzk02.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB19606 (n1)StayGold(c4)/pRSET DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content 明るく、退色しにくい新規の緑色蛍光タンパク質 「StayGold」 (Japanese text)
            Featured content StayGold, highly photostable GFP (English text)
            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19606_B2Eap1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: 6xHis,T7 tag,Xpress tag,insert 5'
            Sequence file: RDB19606_B2Eaa.seq check
            >D08836A2_B2Ea_3_lacIpro-B_F_E05_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNCGNNN CGNGTCNGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC
               61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT
              121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC
              181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA
              241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGA
              301 TGGTGTCTAC AGGCGAGGAG CTGTTTACCG GCGTGGTGCC CTTCAAGTTC CAGCTGAAGG
              361 GCACCATCAA CGGCAAGAGC TTCACCGTGG AAGGCGAGGG CGAGGGCAAT AGCCACGAGG
              421 GCAGCCACAA AGGCAAGTAC GTGTGCACCA GCGGCAAACT GCCAATGTCT TGGGCCGCCC
              481 TGGGAACTAG CTTCGGCTAT GGCATGAAGT ACTACACCAA GTACCCCAGC GGCCTGAAGA
              541 ACTGGTTCCA CGAGGTGATG CCCGAGGGCT TCACCTACGA CAGACACATC CAGTACAAGG
              601 GCGACGGCAG CATCCACGCC AAGCACCAGC ACTTCATGAA GAACGGCACC TACCACAACA
              661 TCGTGGAGTT CACCGGCCAG GACTTCAAGG AGAACAGCCC CGTGCTGACC GGCGACATGA
              721 ACGTGAGCCT GCCCAACGAG GTGCAGCACA TCCCCAGAGA TGACGGCGTG GAGTGNCCAG
              781 TGACCCTGCT GTACCCTCTG CTGAGCGACA AGAGCAAGTG CGTGGANGCC CACCAGAACA
              841 CCATCTGCNA GCCCCTGCAC AATCAGCCAG CCCCCGATGT GNCNTACCAC NNGATCAGAA
              901 AGCAGTACAC CCAGANCAAG GNNGANNCCN NNGANAGAGA NCACATCTGN CANANCGAGN
              961 NCCNGNANNN CNNNCNGNCN NNNNNCNANC CTTCNNNNNC NGNCGNGNNA NANNTNNNNN
            1021 NNNNN
            //
            Primer: f1ori_R3
            Region: T7 ter,insert 3'
            Sequence file: RDB19606_B2Eab.seq check
            >D08836A2_B2Ea_3_f1ori_R3_F05_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNA NNGTTGGGAN GGCGATCGGT GCGGGCCTCT TCGCTATTAC
               61 GCCAGATCCG GATATAGTTC CTCCTTTCAG CAAAAAACCC CTCAAGACCC GTTTAGAGGC
              121 CCCAAGGGGT TATGCTAGTT ATTGCTCAGC GGTGGCAGCA GCCAACTCAG CTTCCTTTCG
              181 GGCTTTGTTA GCAGCCGGAT CAAGCTTCGA ATTCTTACAC GGCAGAAGCA GAAGGCTCGT
              241 GCCATGGCAG GTGGGCCTCC AGGGTCTCGC TCTGGCAGAT GTGGTCTCTC TCCTCGGTGT
              301 CGTCCTTGCT CTGGGTGTAC TGCTTTCTGA TCCAGTGGTA TGGCACATCG GGGGCTGGCT
              361 GATTGTGCAG GGGCTTGCAG ATGGTGTTCT GGTGGGCCTC CACGCACTTG CTCTTGTCGC
              421 TCAGCAGAGG GTACAGCAGG GTCACTGGGC ACTCCACGCC GTCATCTCTG GGGATGTGCT
              481 GCACCTCGTT GGGCAGGCTC ACGTTCATGT CGCCGGTCAG CACGGGGCTG TTCTCCTTGA
              541 AGTCCTGGCC GGTGAACTCC ACGATGTTGT GGTAGGTGCC GTTCTTCATG AAGTGCTGGT
              601 GCTTGGCGTG GATGCTGCCG TCGCCCTTGT ACTGGATGTG TCTGTCGTAG GTGAAGCCCT
              661 CGGGCATCAC CTCGTGGAAC CAGTTCTTCA GGCCGCTGGG GTACTTGGTG TAGTACTTCA
              721 TGCCATAGCC GAAGCTAGTT CCCAGGGCGG CCCAAGACAT TGGCAGTTTG CCGCTGGTGC
              781 ACACGTACTT GCCTTTGTGG CTGCCCTCGT GGCTATTGNC CTCGCCCTCG CCTTCCACGG
              841 TGAAGCTCTT GCCGTTGANG GTGCCNTTCA GCTGGAACTT GAANGGCACC ACGCCGGTAA
              901 ACAGCTCCTC GCCNGTAGAN NCCNTCGGAT NCTTATCGTC ATCGNCNNAC AGNTNCNNAC
              961 CNNTTTGCTG NNCNCCAGTC NNGCTANCCN TANNNNGNTG ANGNNGATGA NGAGANNNNN
            1021 NNNNATGTAT ATCNNCNNNN NNNANGNTNA ANNAANTNNN TNNNNNNNNN N
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

            2022.08.04

            GNP_filter3_RDBDEP_html_220727.pl