Please review the QC test results indicated by icon below as well as clone information before placing your order.

mt-mKeima/pcDNA3

Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15456_A7Jvp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,insert5'(COX8 x2,mKeima(h)) Sequence file: RDB15456_A7Jva.seq
>D05018B7-2_A7Jv_CMV-Forward_A03_03_ABI24.ab1     1 TGTCACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA   121 CTCACTATAG GGAGACCCAA GCTTGGTACC ATGTCCGTCC TGACGCCGCT GCTGCTGCGG   181 GGCTTGACAG GCTCGGCCCG GCGGCTCCCA GTGCCGCGCG CCAAGATCCA TTCGTTGCCG   241 CCGGAGGGGA AGCTTGGGAT GTCCGTCCTG ACGCCGCTGC TGCTGCGGGG CTTGACAGGC   301 TCGGCCCGGC GGCTCCCAGT GCCGCGCGCC AAGATCCATT CGTTGCCGCC GGAGGGGAAG   361 CTCGGGGGAT CCGCCATGGT GAGCGTGATC GCCAAGCAGA TGACCTACAA GGTGTACATG   421 AGCGGCACCG TGAACGGCCA CTACTTCGAG GTGGAGGGCG ACGGCAAGGG CAAGCCCTAC   481 GAGGGCGAGC AGACCGTGAA GCTGACCGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG   541 GACATCCTGA GCCCCCAGCT CCAGTACGGC AGCATCCCCT TCACCAAGTA CCCCGAGGAC   601 ATCCCCGACT ACTTCAAGCA GAGCTTCCCC GAGGGCTACA CCTGGGAGCG CAGCATGAAC   661 TTCGAGGACG GCGCCGTGTG CACCGTGAGC AACGACAGCA GCATCCAGGG CAACTGCTTC   721 ATCTACAACG TGAAGATCAG CGGCGAGAAC TTCCCCCCCA ACGGCCCCGT GATGCAGAAG   781 AAGACCCAGG GCTGGGAGCC CAGCACCGAG CGCCTGTTCG CCCGCGACGG AATGCTGATC   841 GGCAACGACT ACATGGCCCT GAAGCTGGAG GGCGGCGGCC ACTACCTGTG CGAGTTCAAG   901 AGCACCTACA AGGCCAAGAA GCCCGTGAGG ATGCCCGGCC GCCACGAGAT CGACCGCAAG   961 CTGGACGTGA CCAGCCCACA ACCGCGACTA CACCAGCGTG GAGCAGTGCG AGATCGCCCA  1021 TCGCCCGCCA CTCCCTGCTG GGGCTAAGAA TTCTGCAGAT ATCATCACAC TGGTGGCCGC  1081 TCGAAGCATG CCATCCTAGA GGGGCCCGTA ATTCCTATAG TGATCATCAT AATGCTAGAG 1141 CTTCGACTGC AAGTTTCG //
Primer: BGH_rev2 Region: bGHp(A),insert3'(mKeima(h),COX8) Sequence file: RDB15456_A7Jvb.seq
>D05018B7-2_A7Jv_BGH_rev2_B03_06_ABI24.ab1     1 CTTTGTGGGT TAGGAGGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC    61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT   121 AGATGCATGC TCGAGCGGCC GCCAGTGTGA TGGATATCTG CAGAATTCTT AGCCCAGCAG   181 GGAGTGGCGG GCGATGGCGA TCTCGCACTG CTCCACGCTG GTGTAGTCGC GGTTGTGGCT   241 GGTCACGTCC AGCTTGCGGT CGATCTCGTG GCGGCCGGGC ATCCTCACGG GCTTCTTGGC   301 CTTGTAGGTG CTCTTGAACT CGCACAGGTA GTGGCCGCCG CCCTCCAGCT TCAGGGCCAT   361 GTAGTCGTTG CCGATCAGCA TTCCGTCGCG GGCGAACAGG CGCTCGGTGC TGGGCTCCCA   421 GCCCTGGGTC TTCTTCTGCA TCACGGGGCC GTTGGGGGGG AAGTTCTCGC CGCTGATCTT   481 CACGTTGTAG ATGAAGCAGT TGCCCTGGAT GCTGCTGTCG TTGCTCACGG TGCACACGGC   541 GCCGTCCTCG AAGTTCATGC TGCGCTCCCA GGTGTAGCCC TCGGGGAAGC TCTGCTTGAA   601 GTAGTCGGGG ATGTCCTCGG GGTACTTGGT GAAGGGGATG CTGCCGTACT GGAGCTGGGG   661 GCTCAGGATG TCCCAGGCGA AGGGCAGGGG GCCACCCTTG GTCACGGTCA GCTTCACGGT   721 CTGCTCGCCC TCGTAGGGCT TGCCCTTGCC GTCGCCCTCC ACCTCGAAGT AGTGGCCGTT   781 CACGGTGCCG CTCATGTACA CCTTGTAGGT CATCTGCTTG GCGATCACGC TCACCATGGC   841 GGATCCCCCG AGCTTCCCCT CCGGCGGCAA CGAATGGATC TTGGCGCGCG GCACTGGGAG   901 CCGCCGGGCC GAGCCTGTCA AGCCCCGCAG CAGCAGCGGC GTCAGGACGG ACATCCCAAG   961 CTTCCCCTCC GGCGGCAACG AATGGGATCT TGGGCGCGCG GCACTGGGAG CCGCCGGGCC  1021 GAGCCTGTCA AGCCCCGCAG CAGCAGCGCG TCAGGACGGG ACATGGTACT AGCTTTGGTC  1081 TCCCTAATAA GTTGAGTTCG GTAATAATTT CGGATAAGTC AGTTAAGCAG GTGGGGGATT 1141 TCTCTTCTTT GAG //
Primer: SV40pro_F Region: SV40por_ori,NeoR Sequence file: RDB15456_A7Jvc.seq
>D05018B7_2_A7Jv_SV40pro_F_D03_12_ABI24.ab1     1 ACTGGTCCTG GGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC   121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT   661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA   781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA   841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG   901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG   961 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TTCTATGAAA GGGTTGGGCT  1021 TCGGAATCGT TTTCCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG  1081 GAGTTCTTCG CCCACCCCAA CTTGTTTATT TGCAGCTATA TTGTACCAAT AAAGGCCATA 1141 GCATCACAAA CTTTCCACAA AATTAAAAGG CCAA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2019.12.13

GNP_filter3_RDBDEP_html_191130.pl