Please review the QC test results indicated by icon below as well as clone information before placing your order.

mt-mKeima/pcDNA3

Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. 付加的提供条件： 本件リソースの使用は学術機関での学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15456_A7Jvp1-5.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: T7 pro,insert 5' Sequence file: RDB15456_A7Jvd.seq
>D05018B7-2_A7Jv_CMV-Forward_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNTNNNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTCT CTGNNTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA   121 CTCACTATAG GGAGACCCAA GCTTGGTACC ATGTCCGTCC TGACGCCGCT GCTGCTGCGG   181 GGCTTGACAG GCTCGGCCCG GCGGCTCCCA GTGCCGCGCG CCAAGATCCA TTCGTTGCCG   241 CCGGAGGGGA AGCTTGGGAT GTCCGTCCTG ACGCCGCTGC TGCTGCGGGG CTTGACAGGC   301 TCGGCCCGGC GGCTCCCAGT GCCGCGCGCC AAGATCCATT CGTTGCCGCC GGAGGGGAAG   361 CTCGGGGGAT CCGCCATGGT GAGCGTGATC GCCAAGCAGA TGACCTACAA GGTGTACATG   421 AGCGGCACCG TGAACGGCCA CTACTTCGAG GTGGAGGGCG ACGGCAAGGG CAAGCCCTAC   481 GAGGGCGAGC AGACCGTGAA GCTGACCGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG   541 GACATCCTGA GCCCCCAGCT CCAGTACGGC AGCATCCCCT TCACCAAGTA CCCCGAGGAC   601 ATCCCCGACT ACTTCAAGCA GAGCTTCCCC GAGGGCTACA CCTGGGAGCG CAGCATGAAC   661 TTCGAGGACG GCGCCGTGTG CACCGTGAGC AACGACAGCA GCATCCAGGG CAACTGCTTC   721 ATCTACAACG TGAAGATCAG CGGCGAGAAC TTCCCCCCCA ACGGCCCCGN GATGCAGAAG   781 AAGANCCAGG GCTGGGANCC CAGCACCGAG CGCCTGTTCG CCCGCGACGG ANTGCTGATC   841 GGCAACGANT ACNTGGCCCN NAAGCTGGAN GGCGGCGGCC NNTANCNNNG CNANTNNNNG   901 ANCNCCNANN NNGNCAAGAA GCCCNNNNNG NTGNCCGGNC GCCNCNANNT CNNNCGCAAG 961 CNNNNNNNNN N //
Primer: BGH_rev2 (Pr0606) Region: insert 3' Sequence file: RDB15456_A7Jve.seq
>D05018B7-2_A7Jv_BGH_rev2_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGGNNGGG NNNNNNNCAG ATGGCTGGCA ACTAGAAGGC    61 ACAGTCGAGG CNGNNNNNNN NGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT   121 AGATGCATGC TCGAGCGGCC GCCAGTGTGA TGGATATCTG CAGAATTCTT AGCCCAGCAG   181 GGAGTGGCGG GCGATGGCGA TCTCGCACTG CTCCACGCTG GTGTAGTCGC GGTTGTGGCT   241 GGTCACGTCC AGCTTGCGGT CGATCTCGTG GCGGCCGGGC ATCCTCACGG GCTTCTTGGC   301 CTTGTAGGTG CTCTTGAACT CGCACAGGTA GTGGCCGCCG CCCTCCAGCT TCAGGGCCAT   361 GTAGTCGTTG CCGATCAGCA TTCCGTCGCG GGCGAACAGG CGCTCGGTGC TGGGCTCCCA   421 GCCCTGGGTC TTCTTCTGCA TCACGGGGCC GTTGGGGGGG AAGTTCTCGC CGCTGATCTT   481 CACGTTGTAG ATGAAGCAGT TGCCCTGGAT GCTGCTGTCG TTGCTCACGG TGCACACGGC   541 GCCGTCCTCG AAGTTCATGC TGCGCTCCCA GGTGTAGCCC TCGGGGAAGC TCTGCTTGAA   601 GTAGTCGGGG ATGTCCTCGG GGTACTTGGT GAAGGGGATG CTGCCGTACT GGAGCTGGGG   661 GCTCAGGATG TCCCAGGCGA AGGGCAGGGG GCCACCCTTG GTCACGGTCA GCTTCACGGT   721 CTGCTCGCCC TCGTAGGGCT TGCCCTTGCC GTCGCCCTCC ACCTCGAAGT AGTGGCCGTT   781 CACGGTGCCG CTCATGTACA CCTTGTAGGT CATCTGCTTG GCGATCACGC TCACCATGGC   841 GGANCCCCCG ANCTTCCCCT CCGGCGGCAA CGAATGGATC TTGGCGCGCG GCNCNGGGAG   901 CCGCCGGGNC GANCCTGTCN AGCCCCGCAN CANCAGCGGN NNCNNGNNNN ANNTCCCNAG   961 NNNCCCNNNC NNNGGCNNCN AANNGNATNN NNGNCGNNCG GNNCNNGNNN NNNNNNGNNN 1021 NNNNNNN //
Primer: SV40pro_F (Pr0110) Region: NeoR Sequence file: RDB15456_A7Jvf.seq
>D05018B7_2_A7Jv_SV40pro_F_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN ANNNGTGANG ANGNTTTTTT GGAGGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGAGCTTGTA TATCCNTTTT CGGATCTGAT CAAGAGACAG GATGAGGATC   121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT   661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   721 GGAAAATGGC CGCTTTTCTG GANTCNTCGA CTGTGNCCGG CTGGGTGTGN CGGANCGCTA   781 TCAGGACATA GCGTTGGCTA CNCGTGATAT TGCTGAAGAN CTTGGCGGCG AATGGGCTGA   841 CCGCTTCCTC GTGCTTTNNG GNATCGCCGC TCCCGANTCG CANCGCATCG CCTTCNATCG   901 CCTTCTTGAN NANTTCTTCT GANCGGGNNN CTGGGGTNNN AAATGANNGA NCNANCGACG   961 CCNNACCNGN CNTCACNANN NTNNNNNTNN NCCNNNNNNN TNNTATNAAA NGNNNGGNNN 1021 NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl