RDB15456 - mt-mKeima/pcDNA3

Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15456	mt-mKeima/pcDNA3	DNA solution

original	Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

Featured content	Visualization of Organelles (English text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

original	Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.
reference	Sun, N., A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima. Nat. Protoc. 12 (8): 1576-1587 (2017). PMID 28703790.
reference	Lazarou, M., The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature 524 (7565): 309-314 (2015). PMID 26266977.

Primer: CMV_Forward Region: CMV pro,insert5'(COX8 x2,mKeima(h)) Sequence file: RDB15456_A7Jva.seq
>D05018B7-2_A7Jv_CMV-Forward_A03_03_ABI24.ab1 1 TGTCACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTTGGTACC ATGTCCGTCC TGACGCCGCT GCTGCTGCGG 181 GGCTTGACAG GCTCGGCCCG GCGGCTCCCA GTGCCGCGCG CCAAGATCCA TTCGTTGCCG 241 CCGGAGGGGA AGCTTGGGAT GTCCGTCCTG ACGCCGCTGC TGCTGCGGGG CTTGACAGGC 301 TCGGCCCGGC GGCTCCCAGT GCCGCGCGCC AAGATCCATT CGTTGCCGCC GGAGGGGAAG 361 CTCGGGGGAT CCGCCATGGT GAGCGTGATC GCCAAGCAGA TGACCTACAA GGTGTACATG 421 AGCGGCACCG TGAACGGCCA CTACTTCGAG GTGGAGGGCG ACGGCAAGGG CAAGCCCTAC 481 GAGGGCGAGC AGACCGTGAA GCTGACCGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG 541 GACATCCTGA GCCCCCAGCT CCAGTACGGC AGCATCCCCT TCACCAAGTA CCCCGAGGAC 601 ATCCCCGACT ACTTCAAGCA GAGCTTCCCC GAGGGCTACA CCTGGGAGCG CAGCATGAAC 661 TTCGAGGACG GCGCCGTGTG CACCGTGAGC AACGACAGCA GCATCCAGGG CAACTGCTTC 721 ATCTACAACG TGAAGATCAG CGGCGAGAAC TTCCCCCCCA ACGGCCCCGT GATGCAGAAG 781 AAGACCCAGG GCTGGGAGCC CAGCACCGAG CGCCTGTTCG CCCGCGACGG AATGCTGATC 841 GGCAACGACT ACATGGCCCT GAAGCTGGAG GGCGGCGGCC ACTACCTGTG CGAGTTCAAG 901 AGCACCTACA AGGCCAAGAA GCCCGTGAGG ATGCCCGGCC GCCACGAGAT CGACCGCAAG 961 CTGGACGTGA CCAGCCCACA ACCGCGACTA CACCAGCGTG GAGCAGTGCG AGATCGCCCA 1021 TCGCCCGCCA CTCCCTGCTG GGGCTAAGAA TTCTGCAGAT ATCATCACAC TGGTGGCCGC 1081 TCGAAGCATG CCATCCTAGA GGGGCCCGTA ATTCCTATAG TGATCATCAT AATGCTAGAG 1141 CTTCGACTGC AAGTTTCG //
Primer: BGH_rev2 Region: bGHp(A),insert3'(mKeima(h),COX8) Sequence file: RDB15456_A7Jvb.seq
>D05018B7-2_A7Jv_BGH_rev2_B03_06_ABI24.ab1 1 CTTTGTGGGT TAGGAGGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC 61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT 121 AGATGCATGC TCGAGCGGCC GCCAGTGTGA TGGATATCTG CAGAATTCTT AGCCCAGCAG 181 GGAGTGGCGG GCGATGGCGA TCTCGCACTG CTCCACGCTG GTGTAGTCGC GGTTGTGGCT 241 GGTCACGTCC AGCTTGCGGT CGATCTCGTG GCGGCCGGGC ATCCTCACGG GCTTCTTGGC 301 CTTGTAGGTG CTCTTGAACT CGCACAGGTA GTGGCCGCCG CCCTCCAGCT TCAGGGCCAT 361 GTAGTCGTTG CCGATCAGCA TTCCGTCGCG GGCGAACAGG CGCTCGGTGC TGGGCTCCCA 421 GCCCTGGGTC TTCTTCTGCA TCACGGGGCC GTTGGGGGGG AAGTTCTCGC CGCTGATCTT 481 CACGTTGTAG ATGAAGCAGT TGCCCTGGAT GCTGCTGTCG TTGCTCACGG TGCACACGGC 541 GCCGTCCTCG AAGTTCATGC TGCGCTCCCA GGTGTAGCCC TCGGGGAAGC TCTGCTTGAA 601 GTAGTCGGGG ATGTCCTCGG GGTACTTGGT GAAGGGGATG CTGCCGTACT GGAGCTGGGG 661 GCTCAGGATG TCCCAGGCGA AGGGCAGGGG GCCACCCTTG GTCACGGTCA GCTTCACGGT 721 CTGCTCGCCC TCGTAGGGCT TGCCCTTGCC GTCGCCCTCC ACCTCGAAGT AGTGGCCGTT 781 CACGGTGCCG CTCATGTACA CCTTGTAGGT CATCTGCTTG GCGATCACGC TCACCATGGC 841 GGATCCCCCG AGCTTCCCCT CCGGCGGCAA CGAATGGATC TTGGCGCGCG GCACTGGGAG 901 CCGCCGGGCC GAGCCTGTCA AGCCCCGCAG CAGCAGCGGC GTCAGGACGG ACATCCCAAG 961 CTTCCCCTCC GGCGGCAACG AATGGGATCT TGGGCGCGCG GCACTGGGAG CCGCCGGGCC 1021 GAGCCTGTCA AGCCCCGCAG CAGCAGCGCG TCAGGACGGG ACATGGTACT AGCTTTGGTC 1081 TCCCTAATAA GTTGAGTTCG GTAATAATTT CGGATAAGTC AGTTAAGCAG GTGGGGGATT 1141 TCTCTTCTTT GAG //
Primer: SV40pro_F Region: SV40por_ori,NeoR Sequence file: RDB15456_A7Jvc.seq
>D05018B7_2_A7Jv_SV40pro_F_D03_12_ABI24.ab1 1 ACTGGTCCTG GGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC 121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT 661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG 961 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TTCTATGAAA GGGTTGGGCT 1021 TCGGAATCGT TTTCCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG 1081 GAGTTCTTCG CCCACCCCAA CTTGTTTATT TGCAGCTATA TTGTACCAAT AAAGGCCATA 1141 GCATCACAAA CTTTCCACAA AATTAAAAGG CCAA //