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mt-mKeima/pcDNA3

Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.

Catalog number RDB15456
Resource name mt-mKeima/pcDNA3
Clone info. Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.
Vector backbone pcDNA3 (Plasmid)
Gene/insert name Montipora. sp RFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Montipora. sp RFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
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            RDB15456 mt-mKeima/pcDNA3 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualization of Organelles (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15456_A7Jvp1-4.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro,insert5'(COX8 x2,mKeima(h))
            Sequence file: RDB15456_A7Jva.seq check
            >D05018B7-2_A7Jv_CMV-Forward_A03_03_ABI24.ab1
                1 TGTCACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
               61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
              121 CTCACTATAG GGAGACCCAA GCTTGGTACC ATGTCCGTCC TGACGCCGCT GCTGCTGCGG
              181 GGCTTGACAG GCTCGGCCCG GCGGCTCCCA GTGCCGCGCG CCAAGATCCA TTCGTTGCCG
              241 CCGGAGGGGA AGCTTGGGAT GTCCGTCCTG ACGCCGCTGC TGCTGCGGGG CTTGACAGGC
              301 TCGGCCCGGC GGCTCCCAGT GCCGCGCGCC AAGATCCATT CGTTGCCGCC GGAGGGGAAG
              361 CTCGGGGGAT CCGCCATGGT GAGCGTGATC GCCAAGCAGA TGACCTACAA GGTGTACATG
              421 AGCGGCACCG TGAACGGCCA CTACTTCGAG GTGGAGGGCG ACGGCAAGGG CAAGCCCTAC
              481 GAGGGCGAGC AGACCGTGAA GCTGACCGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG
              541 GACATCCTGA GCCCCCAGCT CCAGTACGGC AGCATCCCCT TCACCAAGTA CCCCGAGGAC
              601 ATCCCCGACT ACTTCAAGCA GAGCTTCCCC GAGGGCTACA CCTGGGAGCG CAGCATGAAC
              661 TTCGAGGACG GCGCCGTGTG CACCGTGAGC AACGACAGCA GCATCCAGGG CAACTGCTTC
              721 ATCTACAACG TGAAGATCAG CGGCGAGAAC TTCCCCCCCA ACGGCCCCGT GATGCAGAAG
              781 AAGACCCAGG GCTGGGAGCC CAGCACCGAG CGCCTGTTCG CCCGCGACGG AATGCTGATC
              841 GGCAACGACT ACATGGCCCT GAAGCTGGAG GGCGGCGGCC ACTACCTGTG CGAGTTCAAG
              901 AGCACCTACA AGGCCAAGAA GCCCGTGAGG ATGCCCGGCC GCCACGAGAT CGACCGCAAG
              961 CTGGACGTGA CCAGCCCACA ACCGCGACTA CACCAGCGTG GAGCAGTGCG AGATCGCCCA
             1021 TCGCCCGCCA CTCCCTGCTG GGGCTAAGAA TTCTGCAGAT ATCATCACAC TGGTGGCCGC
             1081 TCGAAGCATG CCATCCTAGA GGGGCCCGTA ATTCCTATAG TGATCATCAT AATGCTAGAG
            1141 CTTCGACTGC AAGTTTCG
            //
            Primer: BGH_rev2
            Region: bGHp(A),insert3'(mKeima(h),COX8)
            Sequence file: RDB15456_A7Jvb.seq check
            >D05018B7-2_A7Jv_BGH_rev2_B03_06_ABI24.ab1
                1 CTTTGTGGGT TAGGAGGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC
               61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT
              121 AGATGCATGC TCGAGCGGCC GCCAGTGTGA TGGATATCTG CAGAATTCTT AGCCCAGCAG
              181 GGAGTGGCGG GCGATGGCGA TCTCGCACTG CTCCACGCTG GTGTAGTCGC GGTTGTGGCT
              241 GGTCACGTCC AGCTTGCGGT CGATCTCGTG GCGGCCGGGC ATCCTCACGG GCTTCTTGGC
              301 CTTGTAGGTG CTCTTGAACT CGCACAGGTA GTGGCCGCCG CCCTCCAGCT TCAGGGCCAT
              361 GTAGTCGTTG CCGATCAGCA TTCCGTCGCG GGCGAACAGG CGCTCGGTGC TGGGCTCCCA
              421 GCCCTGGGTC TTCTTCTGCA TCACGGGGCC GTTGGGGGGG AAGTTCTCGC CGCTGATCTT
              481 CACGTTGTAG ATGAAGCAGT TGCCCTGGAT GCTGCTGTCG TTGCTCACGG TGCACACGGC
              541 GCCGTCCTCG AAGTTCATGC TGCGCTCCCA GGTGTAGCCC TCGGGGAAGC TCTGCTTGAA
              601 GTAGTCGGGG ATGTCCTCGG GGTACTTGGT GAAGGGGATG CTGCCGTACT GGAGCTGGGG
              661 GCTCAGGATG TCCCAGGCGA AGGGCAGGGG GCCACCCTTG GTCACGGTCA GCTTCACGGT
              721 CTGCTCGCCC TCGTAGGGCT TGCCCTTGCC GTCGCCCTCC ACCTCGAAGT AGTGGCCGTT
              781 CACGGTGCCG CTCATGTACA CCTTGTAGGT CATCTGCTTG GCGATCACGC TCACCATGGC
              841 GGATCCCCCG AGCTTCCCCT CCGGCGGCAA CGAATGGATC TTGGCGCGCG GCACTGGGAG
              901 CCGCCGGGCC GAGCCTGTCA AGCCCCGCAG CAGCAGCGGC GTCAGGACGG ACATCCCAAG
              961 CTTCCCCTCC GGCGGCAACG AATGGGATCT TGGGCGCGCG GCACTGGGAG CCGCCGGGCC
             1021 GAGCCTGTCA AGCCCCGCAG CAGCAGCGCG TCAGGACGGG ACATGGTACT AGCTTTGGTC
             1081 TCCCTAATAA GTTGAGTTCG GTAATAATTT CGGATAAGTC AGTTAAGCAG GTGGGGGATT
            1141 TCTCTTCTTT GAG
            //
            Primer: SV40pro_F
            Region: SV40por_ori,NeoR
            Sequence file: RDB15456_A7Jvc.seq check
            >D05018B7_2_A7Jv_SV40pro_F_D03_12_ABI24.ab1
                1 ACTGGTCCTG GGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
               61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
              121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
              181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
              241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
              301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
              361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
              421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
              481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
              541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
              601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
              661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
              721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
              781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
              841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
              901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
              961 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TTCTATGAAA GGGTTGGGCT
             1021 TCGGAATCGT TTTCCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
             1081 GAGTTCTTCG CCCACCCCAA CTTGTTTATT TGCAGCTATA TTGTACCAAT AAAGGCCATA
            1141 GCATCACAAA CTTTCCACAA AATTAAAAGG CCAA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.
            reference Sun, N., A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima. Nat. Protoc. 12 (8): 1576-1587 (2017). PMID 28703790.
            reference Lazarou, M., The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature 524 (7565): 309-314 (2015). PMID 26266977.

            2021.12.14

            GNP_filter3_RDBDEP_html_211111.pl