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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mt-mKeima/pcDNA3

Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.

Catalog number RDB15456
Resource name mt-mKeima/pcDNA3
Clone info. Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.
Vector backbone pcDNA3 (Plasmid)
Gene/insert name Montipora. sp RFP cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15456 mt-mKeima/pcDNA3 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Organelle marker (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15456_A7Jvp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert5'(COX8 x2,mKeima(h))
Sequence file: RDB15456_A7Jva.seq check
>D05018B7-2_A7Jv_CMV-Forward_A03_03_ABI24.ab1
    1 TGTCACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTTGGTACC ATGTCCGTCC TGACGCCGCT GCTGCTGCGG
  181 GGCTTGACAG GCTCGGCCCG GCGGCTCCCA GTGCCGCGCG CCAAGATCCA TTCGTTGCCG
  241 CCGGAGGGGA AGCTTGGGAT GTCCGTCCTG ACGCCGCTGC TGCTGCGGGG CTTGACAGGC
  301 TCGGCCCGGC GGCTCCCAGT GCCGCGCGCC AAGATCCATT CGTTGCCGCC GGAGGGGAAG
  361 CTCGGGGGAT CCGCCATGGT GAGCGTGATC GCCAAGCAGA TGACCTACAA GGTGTACATG
  421 AGCGGCACCG TGAACGGCCA CTACTTCGAG GTGGAGGGCG ACGGCAAGGG CAAGCCCTAC
  481 GAGGGCGAGC AGACCGTGAA GCTGACCGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG
  541 GACATCCTGA GCCCCCAGCT CCAGTACGGC AGCATCCCCT TCACCAAGTA CCCCGAGGAC
  601 ATCCCCGACT ACTTCAAGCA GAGCTTCCCC GAGGGCTACA CCTGGGAGCG CAGCATGAAC
  661 TTCGAGGACG GCGCCGTGTG CACCGTGAGC AACGACAGCA GCATCCAGGG CAACTGCTTC
  721 ATCTACAACG TGAAGATCAG CGGCGAGAAC TTCCCCCCCA ACGGCCCCGT GATGCAGAAG
  781 AAGACCCAGG GCTGGGAGCC CAGCACCGAG CGCCTGTTCG CCCGCGACGG AATGCTGATC
  841 GGCAACGACT ACATGGCCCT GAAGCTGGAG GGCGGCGGCC ACTACCTGTG CGAGTTCAAG
  901 AGCACCTACA AGGCCAAGAA GCCCGTGAGG ATGCCCGGCC GCCACGAGAT CGACCGCAAG
  961 CTGGACGTGA CCAGCCCACA ACCGCGACTA CACCAGCGTG GAGCAGTGCG AGATCGCCCA
 1021 TCGCCCGCCA CTCCCTGCTG GGGCTAAGAA TTCTGCAGAT ATCATCACAC TGGTGGCCGC
 1081 TCGAAGCATG CCATCCTAGA GGGGCCCGTA ATTCCTATAG TGATCATCAT AATGCTAGAG
1141 CTTCGACTGC AAGTTTCG
//
Primer: BGH_rev2
Region: bGHp(A),insert3'(mKeima(h),COX8)
Sequence file: RDB15456_A7Jvb.seq check
>D05018B7-2_A7Jv_BGH_rev2_B03_06_ABI24.ab1
    1 CTTTGTGGGT TAGGAGGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT
  121 AGATGCATGC TCGAGCGGCC GCCAGTGTGA TGGATATCTG CAGAATTCTT AGCCCAGCAG
  181 GGAGTGGCGG GCGATGGCGA TCTCGCACTG CTCCACGCTG GTGTAGTCGC GGTTGTGGCT
  241 GGTCACGTCC AGCTTGCGGT CGATCTCGTG GCGGCCGGGC ATCCTCACGG GCTTCTTGGC
  301 CTTGTAGGTG CTCTTGAACT CGCACAGGTA GTGGCCGCCG CCCTCCAGCT TCAGGGCCAT
  361 GTAGTCGTTG CCGATCAGCA TTCCGTCGCG GGCGAACAGG CGCTCGGTGC TGGGCTCCCA
  421 GCCCTGGGTC TTCTTCTGCA TCACGGGGCC GTTGGGGGGG AAGTTCTCGC CGCTGATCTT
  481 CACGTTGTAG ATGAAGCAGT TGCCCTGGAT GCTGCTGTCG TTGCTCACGG TGCACACGGC
  541 GCCGTCCTCG AAGTTCATGC TGCGCTCCCA GGTGTAGCCC TCGGGGAAGC TCTGCTTGAA
  601 GTAGTCGGGG ATGTCCTCGG GGTACTTGGT GAAGGGGATG CTGCCGTACT GGAGCTGGGG
  661 GCTCAGGATG TCCCAGGCGA AGGGCAGGGG GCCACCCTTG GTCACGGTCA GCTTCACGGT
  721 CTGCTCGCCC TCGTAGGGCT TGCCCTTGCC GTCGCCCTCC ACCTCGAAGT AGTGGCCGTT
  781 CACGGTGCCG CTCATGTACA CCTTGTAGGT CATCTGCTTG GCGATCACGC TCACCATGGC
  841 GGATCCCCCG AGCTTCCCCT CCGGCGGCAA CGAATGGATC TTGGCGCGCG GCACTGGGAG
  901 CCGCCGGGCC GAGCCTGTCA AGCCCCGCAG CAGCAGCGGC GTCAGGACGG ACATCCCAAG
  961 CTTCCCCTCC GGCGGCAACG AATGGGATCT TGGGCGCGCG GCACTGGGAG CCGCCGGGCC
 1021 GAGCCTGTCA AGCCCCGCAG CAGCAGCGCG TCAGGACGGG ACATGGTACT AGCTTTGGTC
 1081 TCCCTAATAA GTTGAGTTCG GTAATAATTT CGGATAAGTC AGTTAAGCAG GTGGGGGATT
1141 TCTCTTCTTT GAG
//
Primer: SV40pro_F
Region: SV40por_ori,NeoR
Sequence file: RDB15456_A7Jvc.seq check
>D05018B7_2_A7Jv_SV40pro_F_D03_12_ABI24.ab1
    1 ACTGGTCCTG GGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
  961 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TTCTATGAAA GGGTTGGGCT
 1021 TCGGAATCGT TTTCCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
 1081 GAGTTCTTCG CCCACCCCAA CTTGTTTATT TGCAGCTATA TTGTACCAAT AAAGGCCATA
1141 GCATCACAAA CTTTCCACAA AATTAAAAGG CCAA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.
reference Sun, N., A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima. Nat. Protoc. 12 (8): 1576-1587 (2017). PMID 28703790.
reference Lazarou, M., The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature 524 (7565): 309-314 (2015). PMID 26266977.

2019.12.13

GNP_filter3_RDBDEP_html_191130.pl