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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mt-mKeima/pcDNA3

Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.

Catalog number RDB15456
Resource name mt-mKeima/pcDNA3
Clone info. Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.
Vector backbone pcDNA3 (Plasmid)
Gene/insert name Montipora. sp RFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Montipora. sp RFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            付加的提供条件:
            本件リソースの使用は学術機関での学術研究に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15456 mt-mKeima/pcDNA3 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualization of Organelles (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15456_A7Jvp1-5.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: T7 pro,insert 5'
            Sequence file: RDB15456_A7Jvd.seq check
            >D05018B7-2_A7Jv_CMV-Forward_A03_03_ABI24.ab1
                1 NNNNNNNNNN NNNNNTNNNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
               61 GCAGAGCTCT CTGNNTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
              121 CTCACTATAG GGAGACCCAA GCTTGGTACC ATGTCCGTCC TGACGCCGCT GCTGCTGCGG
              181 GGCTTGACAG GCTCGGCCCG GCGGCTCCCA GTGCCGCGCG CCAAGATCCA TTCGTTGCCG
              241 CCGGAGGGGA AGCTTGGGAT GTCCGTCCTG ACGCCGCTGC TGCTGCGGGG CTTGACAGGC
              301 TCGGCCCGGC GGCTCCCAGT GCCGCGCGCC AAGATCCATT CGTTGCCGCC GGAGGGGAAG
              361 CTCGGGGGAT CCGCCATGGT GAGCGTGATC GCCAAGCAGA TGACCTACAA GGTGTACATG
              421 AGCGGCACCG TGAACGGCCA CTACTTCGAG GTGGAGGGCG ACGGCAAGGG CAAGCCCTAC
              481 GAGGGCGAGC AGACCGTGAA GCTGACCGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG
              541 GACATCCTGA GCCCCCAGCT CCAGTACGGC AGCATCCCCT TCACCAAGTA CCCCGAGGAC
              601 ATCCCCGACT ACTTCAAGCA GAGCTTCCCC GAGGGCTACA CCTGGGAGCG CAGCATGAAC
              661 TTCGAGGACG GCGCCGTGTG CACCGTGAGC AACGACAGCA GCATCCAGGG CAACTGCTTC
              721 ATCTACAACG TGAAGATCAG CGGCGAGAAC TTCCCCCCCA ACGGCCCCGN GATGCAGAAG
              781 AAGANCCAGG GCTGGGANCC CAGCACCGAG CGCCTGTTCG CCCGCGACGG ANTGCTGATC
              841 GGCAACGANT ACNTGGCCCN NAAGCTGGAN GGCGGCGGCC NNTANCNNNG CNANTNNNNG
              901 ANCNCCNANN NNGNCAAGAA GCCCNNNNNG NTGNCCGGNC GCCNCNANNT CNNNCGCAAG
            961 CNNNNNNNNN N
            //
            Primer: BGH_rev2
            Region: insert 3'
            Sequence file: RDB15456_A7Jve.seq check
            >D05018B7-2_A7Jv_BGH_rev2_B03_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGGGNNGGG NNNNNNNCAG ATGGCTGGCA ACTAGAAGGC
               61 ACAGTCGAGG CNGNNNNNNN NGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT
              121 AGATGCATGC TCGAGCGGCC GCCAGTGTGA TGGATATCTG CAGAATTCTT AGCCCAGCAG
              181 GGAGTGGCGG GCGATGGCGA TCTCGCACTG CTCCACGCTG GTGTAGTCGC GGTTGTGGCT
              241 GGTCACGTCC AGCTTGCGGT CGATCTCGTG GCGGCCGGGC ATCCTCACGG GCTTCTTGGC
              301 CTTGTAGGTG CTCTTGAACT CGCACAGGTA GTGGCCGCCG CCCTCCAGCT TCAGGGCCAT
              361 GTAGTCGTTG CCGATCAGCA TTCCGTCGCG GGCGAACAGG CGCTCGGTGC TGGGCTCCCA
              421 GCCCTGGGTC TTCTTCTGCA TCACGGGGCC GTTGGGGGGG AAGTTCTCGC CGCTGATCTT
              481 CACGTTGTAG ATGAAGCAGT TGCCCTGGAT GCTGCTGTCG TTGCTCACGG TGCACACGGC
              541 GCCGTCCTCG AAGTTCATGC TGCGCTCCCA GGTGTAGCCC TCGGGGAAGC TCTGCTTGAA
              601 GTAGTCGGGG ATGTCCTCGG GGTACTTGGT GAAGGGGATG CTGCCGTACT GGAGCTGGGG
              661 GCTCAGGATG TCCCAGGCGA AGGGCAGGGG GCCACCCTTG GTCACGGTCA GCTTCACGGT
              721 CTGCTCGCCC TCGTAGGGCT TGCCCTTGCC GTCGCCCTCC ACCTCGAAGT AGTGGCCGTT
              781 CACGGTGCCG CTCATGTACA CCTTGTAGGT CATCTGCTTG GCGATCACGC TCACCATGGC
              841 GGANCCCCCG ANCTTCCCCT CCGGCGGCAA CGAATGGATC TTGGCGCGCG GCNCNGGGAG
              901 CCGCCGGGNC GANCCTGTCN AGCCCCGCAN CANCAGCGGN NNCNNGNNNN ANNTCCCNAG
              961 NNNCCCNNNC NNNGGCNNCN AANNGNATNN NNGNCGNNCG GNNCNNGNNN NNNNNNGNNN
            1021 NNNNNNN
            //
            Primer: SV40pro_F
            Region: NeoR
            Sequence file: RDB15456_A7Jvf.seq check
            >D05018B7_2_A7Jv_SV40pro_F_D03_12_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN ANNNGTGANG ANGNTTTTTT GGAGGCCTAG GCTTTTGCAA
               61 AAAGCTCCCG GGAGCTTGTA TATCCNTTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
              121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
              181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
              241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
              301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
              361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
              421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
              481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
              541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
              601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
              661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
              721 GGAAAATGGC CGCTTTTCTG GANTCNTCGA CTGTGNCCGG CTGGGTGTGN CGGANCGCTA
              781 TCAGGACATA GCGTTGGCTA CNCGTGATAT TGCTGAAGAN CTTGGCGGCG AATGGGCTGA
              841 CCGCTTCCTC GTGCTTTNNG GNATCGCCGC TCCCGANTCG CANCGCATCG CCTTCNATCG
              901 CCTTCTTGAN NANTTCTTCT GANCGGGNNN CTGGGGTNNN AAATGANNGA NCNANCGACG
              961 CCNNACCNGN CNTCACNANN NTNNNNNTNN NCCNNNNNNN TNNTATNAAA NGNNNGGNNN
            1021 NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.
            reference Sun, N., A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima. Nat. Protoc. 12 (8): 1576-1587 (2017). PMID 28703790.
            reference Lazarou, M., The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature 524 (7565): 309-314 (2015). PMID 26266977.

            2022.10.11

            GNP_filter3_RDBDEP_html_221010.pl