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mCherry-hCdt1(30/120)/ pCSII-EF

Lentivirus vector plasmid of Fucci2-G1 phase probe. Component of Fucci2 cell cycle probe.

Catalog number RDB15273
Resource name mCherry-hCdt1(30/120)/ pCSII-EF
Clone info. Lentivirus vector plasmid of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe. Fluorescence was detected in the nucleus.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name human CDT1 cDNA Discosoma sp. RFP cDNA
Reference of insert sequence AB512478 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
human CDT1 Discosoma sp. RFP

          Reference sequence
            
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
          Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
          Ordering Please visit Ordering instruction.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
          付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 【追加提供依頼書】受入れ確認書(書式J)
          このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          提供条件は英文のまま記載してください。提供条件の言語は英語のみ。
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15273 mCherry-hCdt1(30/120)/ pCSII-EF DNA solution

          Ordering instruction of plasmids [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression [Japanese text]. The journal of Japanese Biochemical Society (Seikagaku) 84 (1): 47-52 (2012). PMID 22416473.
          original Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15273_A7H3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Region: EF-1alpha pro, insert5'
          Sequence file: RDB15273_A7H3a.seq check
          >D04498I4_2_A7H3_EF1a-C_F_G01_19_ABI24.ab1
              1 TCATGGGATT GCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT
             61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGCT ACCGGTCTCG AGAATTCGCC
            121 ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC
            181 AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG
            241 GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG
            301 CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG
            361 CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG
            421 CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG
            481 GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC
            541 GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC
            601 GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC
            661 GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC
            721 GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC
            781 GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GGGATATCCA
            841 TCACACTGGC GGCCGCTCGA GCCCAGCCCC GCCAGGCCCG CACTCCGCGC CCCGGCCTCC
            901 GCTACCAGTG GCAGCCGCAA GCGCGCCCGC CCGCCCGCCG CCCCCGGACG CGACCAGGCC
            961 AGGCCACCGG GCCCGCAGAG ACTGCGGCTG TCGGTGGACG AGGTTTCCAG CCCCAGTACC
           1021 CCCGGAGCCC CAGACATCCC AGCCCTGCCC TTCTCCGGGC CAGAAGATAA GAATCCACCC
           1081 CCGGCAGCAG TCAGCCGCCC CCACTGACAT CCGCGCAGGA CAGACTCATC TATCTAGAGT
          1141 TACATCGAAG GATCAAGGCT TTAATCGAAT AATTCAATGC
          //
          Primer: PRE_rev
          Region: PRE, insert3'
          Sequence file: RDB15273_A7H3b.seq check
          >D04498I4-2_A7H3_PRE_rev_G02_20_ABI24.ab1
              1 ACGGCATTCA CATAGCGTAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA
             61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
            121 AGATGGTGTC CTGGTCCTGC GCGGATGTCA GGTGGGGCGG CTGACCTGCT GCCGGGGTGG
            181 ATTTCTTTAT CTTCTGGCCC GGAGAAGGGC AGGCTGGGAT GTCTGGGGCC TCGGGGGTAC
            241 TGGGGCTGGA AACCTCGTCC ACCGACAGCC GCAGTCTCCT GCGGGCCGGT GGCCTGGCCT
            301 GGTCGCGTCC GGGGGCGGCG GGCGGGCGGG CGCGCTTGCG GCTGCCACTG GTAGCGGAGG
            361 CCGGGGCGCG GAGTGCGGGC CTGGCGGGGC TGGGCTCGAG CGGCCGCCAG TGTGATGGAT
            421 ATCCCTTGTA CAGCTCGTCC ATGCCGCCGG TGGAGTGGCG GCCCTCGGCG CGTTCGTACT
            481 GTTCCACGAT GGTGTAGTCC TCGTTGTGGG AGGTGATGTC CAACTTGATG TTGACGTTGT
            541 AGGCGCCGGG CAGCTGCACG GGCTTCTTGG CCTTGTAGGT GGTCTTGACC TCAGCGTCGT
            601 AGTGGCCGCC GTCCTTCAGC TTCAGCCTCT GCTTGATCTC GCCCTTCAGG GCGCCGTCCT
            661 CGGGGTACAT CCGCTCGGAG GAGGCCTCCC AGCCCATGGT CTTCTTCTGC ATTACGGGGC
            721 CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT TCACCTTGTA GATGAACTCG CCGTCCTGCA
            781 GGGAGGAGTC CTGGGTCACG GTCACCACGC CGCCGTCCTC GAAGTTCATC ACGCGCTCCC
            841 ACTTGAAGCC CTCGGGGAAG GACAGCTTCA AGTAGTCGGG GATGTCGGCG GGGTGCTTCA
            901 CGTAGGCCTT GGAGCCGTAC ATGAACTGAG GGACAGGATG TCCCAGGCGA AAGGGCAGGG
            961 GCCACCCTTG GTCACCTTCA GCTTGGCGGG TCTGGGTGCC CTCGTAGGGG CGGGCCCTCG
           1021 CTCTCGCCCT CGATCTCGAA CTCGTGGCCG TTCACGGAGC CTCCATGTGC ACCTTTGAAG
           1081 CGCATGGAAC TCCATGGATG ATGGGCCATG GTATCTTCCT CGCCCTTGCT CACCATGTGG
          1141 CGATTCTCTC GGAACCGTAG CGATGTCACG GAACACATGC CTG
          //
          Primer: CMV_Forward
          Region: CMV pro, 5'LTR, HIV-1psi
          Sequence file: RDB15273_A7H3c.seq check
          >D04498I4-2_A7H3_CMV-Forward_G03_21_ABI24.ab1
              1 TGGTCACCTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
            121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
            181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
            241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
            301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
            361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
            421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
            481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
            541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
            601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
            661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
            721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG
            781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT
            841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG
            901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGG AGCTTTGTTC CTTGGGTTCT TGGGAGCAGC
            961 AGGAAGCACT ATGGGCGCAG CGTCAATGAC GCTGACGGTA CAGGCCAGAC ATTATTGTCT
           1021 GGTATAGTGC AGCAGCAGAA CAATTTGCTG AGGGCTTATT GAGCGCAACA GCATCTGTTG
           1081 CAACTCACAG TCTGGGGGCA ATCAAGCAGC TCAAGCCAAG AATCCTGGAC TGTGTGGAAA
          1141 GGAAAATTAC CCTTAAAAAG
          //
          Primer: BGH_rev2
          Region: bGH p(A), 3'LTR, PRE
          Sequence file: RDB15273_A7H3d.seq check
          >D04498I4-2_A7H3_BGH_rev2_G04_19_ABI24.ab1
              1 CCTTTCAACG ATCAGGTAGC ACGGGGGAGG GGCAACAACA GATGGCTGGC AACTAGAAGG
             61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
            121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
            181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
            241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
            301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
            361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
            421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
            481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
            541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
            601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
            661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
            721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
            781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGAGGGCGAA
            841 GGCGAAGACG CGGAAGAGGC CGCAGAGCCG GCAGCAGGCC GCGGGAAGGA AAGGTCCGCT
            901 GGATTGAGGG CCGAAAGGGA CGTAGCAGAA GGACGTCCCG CGCAGAATCC AGGTGGCAAC
            961 ACAGGCGAGC AGCCATGGGA AAGGGACGTC AGCTTCCCCG ACACACCACG GAATTGTCAG
           1021 TGCCCAACAG CCGAGCCCCT GTCCAGCAGC GGGCAGCAGC GCGATGAGTC CGCCGTGTCA
           1081 TAGGAGGGGG AAGCGAAGTC CGGATGGAGC TGACTGGTGG TGGCCATGCC CACCCAGCGT
          1141 GGGGGGATTG GGCGTTCAGC
          //
          Primer: SV40pro_F
          Region: SV40pro_ori, Zeo, SV40 p(A)
          Sequence file: RDB15273_A7H3e.seq check
          >D04498I4-2_A7H3_SV40pro_F_G05_20_ABI24.ab1
              1 CCCGTCCCGG ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
             61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
            121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
            181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
            241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
            301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
            361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
            421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
            481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
            541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
            601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
            661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
            721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
            781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
            841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
            901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT
            961 AATTTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT
           1021 AAATGAATCG GCCCAACGCC GCGGGGGAGA GGCGGTTGCG TATTGGGGCG CTCTTCCGCT
           1081 TCCTCGCCTC ACTGACTCGC TGCGCTCGGT TCGGTCCGGC CTGCCGGCGG AGCGGTAATC
          1141 AGCCTCCACC TTCCAAAAGA GGCCCGGTTT AT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression [Japanese text]. The journal of Japanese Biochemical Society (Seikagaku) 84 (1): 47-52 (2012). PMID 22416473.
          original Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962.
          reference Singer, D., Prominin-1 controls stem cell activation by orchestrating ciliary dynamics. EMBO J. 38 (2). pii: e99845 (2019). PMID 30523147.
          reference Liu, Y., Repair-independent functions of DNA-PKcs protect irradiated cells from mitotic slippage and accelerated senescence. J. Cell Sci. 132 (13). pii: jcs229385 (2019). PMID 31189537.
          reference Tan, Z., Mammalian Cells Undergo Endoreduplication in Response to Lactic Acidosis. Sci Rep. 8 (1): 2890 (2018). PMID 29440645.
          reference Ohgushi, M., A RHO Small GTPase Regulator ABR Secures Mitotic Fidelity in Human Embryonic Stem Cells. Stem Cell Reports 9 (1): 58-66 (2017). PMID 28579391.
          reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.
          reference Oki, T., A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. Sci. Rep. 4: 4012 (2014). PMID 24500246.
          reference Abe, T., Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter. Development 140 (1): 237-246 (2013). PMID 23175634.
          reference Nishimura, K., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl