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mCherry-hCdt1(30/120)/ pCSII-EF (#RDB15273)

Lentivirus vector plasmid of Fucci2-G1 phase probe. Component of Fucci2 cell cycle probe.

Clone info. Lentivirus vector plasmid of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe. Fluorescence was detected in the nucleus.
Vector backbone CSII-EF-MCS (RDB04378) (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name human CDT1 cDNA Discosoma sp. RFP cDNA
Reference of insert sequence AB512478 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15273hts01.seq checkAssembled from experimentally sequenced data.
Publication Sakaue-Sawano, A., The journal of Japanese Biochemical Society (Seikagaku) 84 (1): 47-52 (2012).
Publication Sakaue-Sawano, A., BMC Cell Biol. 12: 2 (2011). [link to RRC of NBRP]
Test sheet RDB15273_A7J4p1-3.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks Remember that you will be working with samples containing infectious virus.
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
MTAに書く付加的使用条件：
レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考 【追加提供依頼書】受入れ確認書(書式J)
このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
提供条件は英文のまま記載してください。提供条件の言語は英語のみ。
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15273 mCherry-hCdt1(30/120)/ pCSII-EF DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The mCherry-hCdt1(30/120)/ pCSII-EF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15273).

Reference section:

Sakaue-Sawano, A., Miyawaki, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression [Japanese text]. The journal of Japanese Biochemical Society (Seikagaku) 84 (1): 47-52 (2012). PMID 22416473.
Sakaue-Sawano, A., Kobayashi, T., Ohtawa, K., Miyawaki, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression [Japanese text]. The journal of Japanese Biochemical Society (Seikagaku) 84 (1): 47-52 (2012). PMID 22416473.
original Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]
reference Singer, D., Prominin-1 controls stem cell activation by orchestrating ciliary dynamics. EMBO J. 38 (2). pii: e99845 (2019). PMID 30523147.
reference Liu, Y., Repair-independent functions of DNA-PKcs protect irradiated cells from mitotic slippage and accelerated senescence. J. Cell Sci. 132 (13). pii: jcs229385 (2019). PMID 31189537.
reference Tan, Z., Mammalian Cells Undergo Endoreduplication in Response to Lactic Acidosis. Sci Rep. 8 (1): 2890 (2018). PMID 29440645.
reference Ohgushi, M., A RHO Small GTPase Regulator ABR Secures Mitotic Fidelity in Human Embryonic Stem Cells. Stem Cell Reports 9 (1): 58-66 (2017). PMID 28579391.
reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.
reference Oki, T., A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. Sci. Rep. 4: 4012 (2014). PMID 24500246.
reference Abe, T., Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter. Development 140 (1): 237-246 (2013). PMID 23175634.
reference Nishimura, K., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789. [link to RRC of NBRP]
user_report Dehghankelishadi, P., Bosutinib high density lipoprotein nanoformulation has potent tumour radiosensitisation effects. J. Nanobiotechnology 21 (1): 102 (2023) PMID 36945003. [link to RRC of NBRP]
user_report Badiee, P., Intratumoral Anti-PD-1 Nanoformulation Improves Its Biodistribution. ACS Appl. Mater. Interfaces 14 (14): 15881-15893 (2022). PMID 35357803. [link to RRC of NBRP]
user_report Dehghankelishadi, P., Formulation of simvastatin within high density lipoprotein enables potent tumour radiosensitisation. J. Control. Release 346: 98-109 (2022). PMID 35447296. [link to RRC of NBRP]