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tFucci(CA)2/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mVenus-hGem(1/110). CMV promoter.

Catalog number RDB15452
Resource name tFucci(CA)2/pCSII-CMV
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mVenus-hGem(1/110). CMV promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of insert sequence LC212776 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Aequorea victoria GFP

        Reference sequence
          
        Lentiviral plasmid list. [link]

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Ordering instruction.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (非営利機関による非営利学術研究用)[Word]
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15452 tFucci(CA)2/pCSII-CMV DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15452_A7Jvp1-3.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CSII-CMV5'
        Sequence file: RDB15452_A7Jva.seq check
        >D05018B3_1_A7Jv_CSII-CMV5'_C12_09_ABI24.ab1
            1 ACGGCAACAT AAGGATCCAC CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC
           61 AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG
          121 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG
          181 ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC
          241 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC
          301 GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC
          361 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC
          421 TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG
          481 CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG
          541 GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG
          601 CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC
          661 ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG
          721 CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGA TGGAGCAGCG CCGCGTCACC
          781 GACTTCTTCG CGCGCCGCCG CCCCGGGCCC CCCCCGCATC GCGCCGCCCA AGCTGGCCTG
          841 CCGCACCCCC CAGCCCCGCC AGGCCCGCAC TCCGCGCCCC GGCCTCCGCT ACCAGTGGCA
          901 GCCGCAAGCG CGCCCGCCCG CCCGCCGCCC CCGGGACGCG ACCAGGCCAG GGCCACCGGG
          961 CCCGCGCGGG CCGCGCGGGC TGTCGGTTGG ACGAGTTTCC CAGCCCCCAG TACCCCCCGG
         1021 AAGCCCCCAG AACATTCCCA GGCCTGATCG TTCCTCGGGT CAGGAAGAAT AGAATCGATT
        1081 CGCGCCAGCG GCGGGCAAGC CAGC
        //
        Primer: mCherry-C_F
        Sequence file: RDB15452_A7Jvb.seq check
        >D05018B3_1_A7Jv_mCherry-C_F_G03_07_ABI08.ab1
            1 ACAAAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC
           61 CCACAACGAG GACTACACCA TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC
          121 CGGCGGCATG GACGAGCTGT ACAAGGGATA TCCATCACAC TGGCGGCCGC TCGAGATGGA
          181 GCAGCGCCGC GTCACCGACT TCTTCGCGCG CCGCCGCCCC GGGCCCCCCC GCATCGCGCC
          241 GCCCAAGCTG GCCTGCCGCA CCCCCAGCCC CGCCAGGCCC GCACTCCGCG CCCCGGCCTC
          301 CGCTACCAGT GGCAGCCGCA AGCGCGCCCG CCCGCCCGCC GCCCCCGGAC GCGACCAGGC
          361 CAGGCCACCG GCCCGCGCGG CCGCGCGGCT GTCGGTGGAC GAGGTTTCCA GCCCCAGTAC
          421 CCCCGAGGCC CCAGACATCC CAGCCTGCCC TTCTCCGGGC CAGAAGATAA AGAAAACCGG
          481 TCGCGCCAAG CGCGGCAGCG GCGCCACCAA CTTCAGCCTG CTGAAGCAGG CCGGCGACGT
          541 GGAGGAGAAC CCCGGCCCCC GCGAATTCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG
          601 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC
          661 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGCTGA TCTGCACCAC
          721 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGGGCTACG GCCTGCAGTG
          781 CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA
          841 AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGGCAACTAC AAGACCCGCG
          901 CCGAGGTGAA GTTCGAGGGG CGACACCCTG GTGAAACCGC ATCGAGCTGA AGGGCATCGA
          961 CTTCAAAGGA GGACGGCAAC ATCCTGGGGG CACAAGCTGG AGTACAACTA CACCAGCCAC
         1021 CAACCGTCCT ATATCAACGG CGGAACCAGG CAGAAGATCT GCATCAGGCT ACCTTCAAGA
        1081 ATCCGTCCCA CAAACAGT
        //
        Primer: PRE_rev
        Sequence file: RDB15452_A7Jvc.seq check
        >D05018B3_1_A7Jv_PRE_rev_E12_15_ABI24.ab1
            1 AGAATCACAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT
           61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTACA
          121 GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA TTTTCTTTAA
          181 TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA CTAGATTCTG
          241 GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA TGTTTCCTTT
          301 TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT GCAGAAGGCT
          361 GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT TTGATTTCTT
          421 CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT GGATATCCCT
          481 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA
          541 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGCTTGGA CTGGTAGCTC AGGTAGTGGT
          601 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA
          661 GCTGCACGCC GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT
          721 TCTGCTTGTC GGCGGTGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC
          781 CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG
          841 TGTCGCCCTC GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTTGAAGAGA
          901 TGGTGCGCTC CTGGACGTAG CCTTTCGGGC ATGGCGGACT TTGAGAAGTC GTGCTGCTTC
          961 ATGTGGTCGG GGTAGCGGGC GAGGCACTGC AGTCGGTAGC TCAGGTGTTC ACGAAGGTGG
         1021 TAGGGCACGG CCAGCTGCGT TGGTTGCAGA TCAGCTTCAG TCAGCCTGCG TAGTGGCCAT
        1081 CGGCCTTGCG A
        //
        Primer: HIVgp_R1
        Sequence file: RDB15452_A7Jvd.seq check
        >D05018B3_1_A7Jv_HIVgp_R1_A12_03_ABI24.ab1
            1 CGGGCTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG
           61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
          121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG
          181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC
          241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT
          301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT
          361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA
          421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT
          481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG
          541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT
          601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC
          661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
          721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
          781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
          841 CTAACCAGAG AGACCCAGTA CAGGCCAAAA CGCGCTGCCT TATATAGACC TCCCCACCGT
          901 ACACGCCCTA CCGCCCATTT GCGTCAATGG GGCCGGAGTT GTTACGACAT TTTGGGAAAG
          961 TCCCGGTTGA TTTTGGTGCC CAAAAACCAA ACCTCCCCAT GACGTCAATG GGGTGGAGAC
         1021 TTGAAATTCC CCCGTGAGTC CAACCCGCTA TCCCACGCCC ATGGAATGTA CCTGCTGAAC
        1081 GCATCACATG TAAATAGCGA ATGACCTATA
        //
        Primer: BGH_rev
        Sequence file: RDB15452_A7Jve.seq check
        >D05018B3_1_A7Jv_BGH_rev_F12_18_ABI24.ab1
            1 GAAATCGGGG GACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG
           61 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT
          121 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT
          181 AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG CCATGTGATG AAATGCTAGG
          241 CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT CATCCATCCC ATGCAGGCTC
          301 ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT CTTCTACCTT CTCTTGCTCA
          361 ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGATCCC TGGCCCTGGT
          421 GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG ATCAAGGATA
          481 TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG
          541 CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCTGAGG TGTGACTGGA
          601 AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT
          661 GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACGGTATCG ATGCGGGGAG
          721 GCGGCCCAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
          781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
          841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGGAC
          901 GTCAGCTTCC CCGACAACAC CACGGGAATT TGTCAGTGCC CAACAGCCCG AGCCCCTGTC
          961 CCAGCAGCGG GCAGCAGCGG CGATGAGTTC GCGTGCCAAT AGGAGGGGGA AAGCGAAGTC
         1021 CGAAAGAGCT GACAGTGTGC CATGCCCATC AACTGGGATG CGTCAGCTAA CACAGTGCAC
        1081 ACCAACGTCC CACGAG
        //
        Primer: SV40pro_F
        Sequence file: RDB15452_A7Jvf.seq check
        >D05018B3_1_A7Jv_SV40pro_F_B12_06_ABI24.ab1
            1 CATTGGTCCC TGGGACATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
           61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
          121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
          181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
          241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
          301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
          361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
          421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
          481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
          541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
          601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
          661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
          721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
          781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG
          841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
          901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGGTG CCTAAATGAG TGAGCTAACT
          961 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCCTG TCGTGCAGCC
         1021 TGCCATTTAA TGAAATCCGG CTACGGCCGC CGGGAGAGGC CGGATTGCCG TATGGGGCGC
        1081 TCTTCCGCTT TCCTTCGG
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl