Resource data sheet
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tFucci(CA)2/pCSII-CMV

Lentivirus expression vector of mCherry-hCdt1(1/100)Cy(-)_P2A_mVenus-hGem(1/110). CMV promoter.

Catalog number RDB15452
Resource name tFucci(CA)2/pCSII-CMV
Clone info. Lentivirus expression vector of mCherry-hCdt1(1/100)Cy(-)_P2A_mVenus-hGem(1/110). CMV promoter.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence LC212776 (DDBJ accession number)
Originator Miyawaki, Atsushi |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15452_A7Jvp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CSII-CMV5' RDB15452_A7Jva.seq
>D05018B3_1_A7Jv_CSII-CMV5'_C12_09_ABI24.ab1
    1 ACGGCAACAT AAGGATCCAC CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC
   61 AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG
  121 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG
  181 ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC
  241 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC
  301 GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC
  361 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC
  421 TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG
  481 CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG
  541 GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG
  601 CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC
  661 ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG
  721 CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGA TGGAGCAGCG CCGCGTCACC
  781 GACTTCTTCG CGCGCCGCCG CCCCGGGCCC CCCCCGCATC GCGCCGCCCA AGCTGGCCTG
  841 CCGCACCCCC CAGCCCCGCC AGGCCCGCAC TCCGCGCCCC GGCCTCCGCT ACCAGTGGCA
  901 GCCGCAAGCG CGCCCGCCCG CCCGCCGCCC CCGGGACGCG ACCAGGCCAG GGCCACCGGG
  961 CCCGCGCGGG CCGCGCGGGC TGTCGGTTGG ACGAGTTTCC CAGCCCCCAG TACCCCCCGG
 1021 AAGCCCCCAG AACATTCCCA GGCCTGATCG TTCCTCGGGT CAGGAAGAAT AGAATCGATT
1081 CGCGCCAGCG GCGGGCAAGC CAGC
//
Primer: mCherry-C_F RDB15452_A7Jvb.seq
>D05018B3_1_A7Jv_mCherry-C_F_G03_07_ABI08.ab1
    1 ACAAAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC
   61 CCACAACGAG GACTACACCA TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC
  121 CGGCGGCATG GACGAGCTGT ACAAGGGATA TCCATCACAC TGGCGGCCGC TCGAGATGGA
  181 GCAGCGCCGC GTCACCGACT TCTTCGCGCG CCGCCGCCCC GGGCCCCCCC GCATCGCGCC
  241 GCCCAAGCTG GCCTGCCGCA CCCCCAGCCC CGCCAGGCCC GCACTCCGCG CCCCGGCCTC
  301 CGCTACCAGT GGCAGCCGCA AGCGCGCCCG CCCGCCCGCC GCCCCCGGAC GCGACCAGGC
  361 CAGGCCACCG GCCCGCGCGG CCGCGCGGCT GTCGGTGGAC GAGGTTTCCA GCCCCAGTAC
  421 CCCCGAGGCC CCAGACATCC CAGCCTGCCC TTCTCCGGGC CAGAAGATAA AGAAAACCGG
  481 TCGCGCCAAG CGCGGCAGCG GCGCCACCAA CTTCAGCCTG CTGAAGCAGG CCGGCGACGT
  541 GGAGGAGAAC CCCGGCCCCC GCGAATTCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG
  601 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC
  661 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGCTGA TCTGCACCAC
  721 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGGGCTACG GCCTGCAGTG
  781 CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA
  841 AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGGCAACTAC AAGACCCGCG
  901 CCGAGGTGAA GTTCGAGGGG CGACACCCTG GTGAAACCGC ATCGAGCTGA AGGGCATCGA
  961 CTTCAAAGGA GGACGGCAAC ATCCTGGGGG CACAAGCTGG AGTACAACTA CACCAGCCAC
 1021 CAACCGTCCT ATATCAACGG CGGAACCAGG CAGAAGATCT GCATCAGGCT ACCTTCAAGA
1081 ATCCGTCCCA CAAACAGT
//
Primer: PRE_rev RDB15452_A7Jvc.seq
>D05018B3_1_A7Jv_PRE_rev_E12_15_ABI24.ab1
    1 AGAATCACAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT
   61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTACA
  121 GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA TTTTCTTTAA
  181 TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA CTAGATTCTG
  241 GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA TGTTTCCTTT
  301 TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT GCAGAAGGCT
  361 GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT TTGATTTCTT
  421 CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT GGATATCCCT
  481 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA
  541 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGCTTGGA CTGGTAGCTC AGGTAGTGGT
  601 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA
  661 GCTGCACGCC GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT
  721 TCTGCTTGTC GGCGGTGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC
  781 CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG
  841 TGTCGCCCTC GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTTGAAGAGA
  901 TGGTGCGCTC CTGGACGTAG CCTTTCGGGC ATGGCGGACT TTGAGAAGTC GTGCTGCTTC
  961 ATGTGGTCGG GGTAGCGGGC GAGGCACTGC AGTCGGTAGC TCAGGTGTTC ACGAAGGTGG
 1021 TAGGGCACGG CCAGCTGCGT TGGTTGCAGA TCAGCTTCAG TCAGCCTGCG TAGTGGCCAT
1081 CGGCCTTGCG A
//
Primer: HIVgp_R1 RDB15452_A7Jvd.seq
>D05018B3_1_A7Jv_HIVgp_R1_A12_03_ABI24.ab1
    1 CGGGCTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG
   61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
  121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG
  181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC
  241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT
  301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT
  361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA
  421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT
  481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG
  541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT
  601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC
  661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
  721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
  781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
  841 CTAACCAGAG AGACCCAGTA CAGGCCAAAA CGCGCTGCCT TATATAGACC TCCCCACCGT
  901 ACACGCCCTA CCGCCCATTT GCGTCAATGG GGCCGGAGTT GTTACGACAT TTTGGGAAAG
  961 TCCCGGTTGA TTTTGGTGCC CAAAAACCAA ACCTCCCCAT GACGTCAATG GGGTGGAGAC
 1021 TTGAAATTCC CCCGTGAGTC CAACCCGCTA TCCCACGCCC ATGGAATGTA CCTGCTGAAC
1081 GCATCACATG TAAATAGCGA ATGACCTATA
//
Primer: BGH_rev RDB15452_A7Jve.seq
>D05018B3_1_A7Jv_BGH_rev_F12_18_ABI24.ab1
    1 GAAATCGGGG GACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG
   61 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT
  121 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT
  181 AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG CCATGTGATG AAATGCTAGG
  241 CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT CATCCATCCC ATGCAGGCTC
  301 ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT CTTCTACCTT CTCTTGCTCA
  361 ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGATCCC TGGCCCTGGT
  421 GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG ATCAAGGATA
  481 TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG
  541 CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCTGAGG TGTGACTGGA
  601 AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT
  661 GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACGGTATCG ATGCGGGGAG
  721 GCGGCCCAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
  781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
  841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGGAC
  901 GTCAGCTTCC CCGACAACAC CACGGGAATT TGTCAGTGCC CAACAGCCCG AGCCCCTGTC
  961 CCAGCAGCGG GCAGCAGCGG CGATGAGTTC GCGTGCCAAT AGGAGGGGGA AAGCGAAGTC
 1021 CGAAAGAGCT GACAGTGTGC CATGCCCATC AACTGGGATG CGTCAGCTAA CACAGTGCAC
1081 ACCAACGTCC CACGAG
//
Primer: SV40pro_F RDB15452_A7Jvf.seq
>D05018B3_1_A7Jv_SV40pro_F_B12_06_ABI24.ab1
    1 CATTGGTCCC TGGGACATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
  121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
  181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
  241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
  301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
  361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
  421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
  481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
  541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
  601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
  661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
  781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG
  841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
  901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGGTG CCTAAATGAG TGAGCTAACT
  961 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCCTG TCGTGCAGCC
 1021 TGCCATTTAA TGAAATCCGG CTACGGCCGC CGGGAGAGGC CGGATTGCCG TATGGGGCGC
1081 TCTTCCGCTT TCCTTCGG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2017.11.15

GNP_filter3_RDBDEP_html_171106.pl