Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Xpa H62Q (#RDB15265)

Plasmid clone of a non-photoconvertivle variant of KikGR.

Clone info. Plasmid clone of a non-photoconvertivle variant of Kikume Green-Red fluorescent protein, KikGR, a crystallizable but non-photoconvertible variant, XpaH62Q.
Vector backbone pcs4 (pCS2+ derivative) (Plasmid)
Selectable markers Amp^r
Gene/insert name Favia favus Kikume Green-Red fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui H, Jinno Y, Shoda K, Tomita A, Matsuda M, Yamashita E, Katayama H, Nakagawa A, Miyawaki A. (2015) A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
宮脇敦史博士による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui H, Jinno Y, Shoda K, Tomita A, Matsuda M, Yamashita E, Katayama H, Nakagawa A, Miyawaki A. (2015) A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考 提供条件は英文のまま記載してください。
宮脇敦史博士の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15265 Xpa H62Q DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Xpa H62Q was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15265).

Reference section:

Tsutsui, H., Jinno, Y., Shoda, K., Tomita, A., Matsuda, M., Yamashita, E., Katayama, H., Nakagawa, A., Miyawaki, A., A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193 (2015). PMID 25773597. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15265_A7H9p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCALNLF1 ()
Region: SV40 p(A),insert,SP6 pro.,CMVIE94 pro.
Sequence file: RDB15265_A7H9a.seq check
>D04498I5-2_A7H9_pAxCALNL_F1_F01_06_ABI08.ab1
    1 TAAAGTTTAT CTTATCATGT CTGGATCTAC GTAATACGAC TCACTATAGT TCTAGAGGCT
   61 CGAGAGGCCT TGAATTCTTA CTTGGCCAGC CTTGGCAGCC CGGAATGAGC TTTAGCATGC
  121 TCATACAGCT TAACCTTGTT GTAATCCTTG TCATGGCTTG TTATCTCCAT ACTATGATCC
  181 ACGAAGTGAT AGTCTGGCAA CTGGACAACC TTCTTTGCTT TGTAAGTAGT TCTGAAGTCA
  241 CATCGGTAAT GGCCACCTCC TTGAAGCAAC AGAGCCATGT TTACATCACC CTTCAGCACT
  301 CCATCACGCA CATACATTTT CTCAGTGGAT GGCTCCCATT TGACGGTCTT CCTCTGCATA
  361 ACTGGACCAT TGGCAGGAAA GTTCACACCA TCAAATCGAA TTTCATTGAC AAAACAGTTG
  421 CTGCCGTCTT TCTTCATCGT TATATTGTTT GTGGCGAGGC AAATTCCCCC GTCTTCGTAA
  481 CTCATGCTTC GTTCCCAAGA ATAACCCTCA GGAAACGACT GCTTGAAGTA GTCTACTATT
  541 TCTTCTGGGT ATTCGACAAA TACCCGGTTG CCGTATTGGA ATGCTGTTGT CAGGATATCG
  601 AAAGCAAAAG GAAGAGGTCC GCCCTCTATG ACTGTCAGGT CCACATTCTG TATTCCCTCG
  661 AAAGGCTGGC CACTTCCTTT CCCTGTAATC ACGAACTTGT GCCCGTTTAC AGCGCCTTCC
  721 ATACGCAGCT CGATCTTCAT TTCTGATGTA ATCACACTCA CCATGGTCGG ATCCTGCAAA
  781 AAGAACAAGT AGCTTGTATT CTATAGTGTC ACCTAAATCA AGCTTGCTCC GACGTCCCCA
  841 GGCAGAATGG CGGTTCCCTA AACGAGCATT GCTTATATAG ACCTCCCATT AGGCACGGCC
  901 TACCGCCCAT TTACGTCAAT GGGAACGCCC ATTTGCGTCA TTGCCCCTCC CCATTGACGT
  961 CAATGGGGGA TGTACTTGGC AGCCATCGCG GCCATTTACC GGCCATTGAC GTCAATGGGG
 1021 AGTACTGCCC AAATGTACCT GCGTACCTTC CATAGTAATG GTACCTGGCT AGCTACCTAA
1081 TAATTAGATA TGATGTACTG GCAAGGTGGG CCATTTACCG CTTCAATTGC AC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsutsui, H., A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193 (2015). PMID 25773597. [link to RRC of NBRP]
reference Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. [link to RRC of NBRP]