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hmKO-K/pRSETB

Plasmid clone of hmKO-K, faster-maturating version of Kusabira orange.

Catalog number RDB15314
Resource name hmKO-K/pRSETB
Alternative name hmKOkappa/pRSETB
Clone info. Plasmid clone of hmKO-K, faster-maturating version of Kusabira orange.
Vector backbone pRSET B (Plasmid)
Gene/insert name Fungia concinna Orange fluorescent protein cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A. (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15314 hmKO-K/pRSETB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15314_A7Htp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15314_A7Hta.seq
>D04498J5_A7H9_1_T7_20bp_G08_20_ABI24.ab1
    1 GAAATCAGTA GTATAATTTT GTTTACTTTA AGAAGGAGAT ATACATATGC GGGGTTCTCA
   61 TCATCATCAT CATCATGGTA TGGCTAGCAT GACTGGTGGA CAGCAAATGG GTCGGGATCT
  121 GTACGACGAT GACGATAAGG ATCCGACCAT GGTGAGCGTG ATCAAGCCCG AGATGAAGAT
  181 GAGGTACTAC ATGGACGGCT CCGTCAATGG GCATGAGTTC ACAATCGAGG GTGAGGGCAC
  241 AGGCAGACCT TACGAGGGAC ATCAGGAGAT GACACTGCGC GTCACAATGG CCGAGGGCGG
  301 GCCAATGCCT TTCGCCTTCG ACCTGGTGTC CCACGTGTTC TGTTACGGCC ACAGAGTTTT
  361 TACCAAGTAC CCAGAAGAGA TCCCAGACTA TTTCAAGCAG GCCTTTCCTG AGGGCCTGTC
  421 CTGGGAGAGG TCCCTGGAGT TCGAGGACGG CGGCTCCGCC TCCGTGAGCG CCCACATCAG
  481 CCTGAGGGGC AACACCTTCT ACCACAAGTC CAAGTTCACC GGCGTGAACT TCCCCGCCGA
  541 CGGCCCCATC ATGCAGAACC AGAGCGTGGA CTGGGAGCCC TCCACCGAGA AGATCACCGC
  601 CAGCGACGGC GTGCTGAAGG GCGACGTGAC CATGTACCTG AAGCTGGAGG GCGGCGGCAA
  661 CCACAAGTGC CAGTTCAAGA CCACCTACAA GGCCGCCAAG GAGATCCTGG AGATGCCCGG
  721 CGACCACTAC ATCGGCCACA GGCTGGTGAG GAAGACCGAG GGCAACATCA CCGAGCAGGT
  781 GGAGGACGCC GTGGCCCACT CCTAAGAATT CGAAGCTTGA TCCGGCTGCT AACAAAGCCC
  841 GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG
  901 CCTCTAAACG GGTCTTGAGG GGTTTTTTTG CTGAAAGGAG GAACTATATC CGGGATCTGG
  961 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTTCCCAA CAGTTTGCGC AGCCCTGAAA
 1021 TGGGCGAATG GGACGCGCCC TGTAGCGGCG CATTAGCCGC CGGCCGGGTT GTTGGTTGAT
1081 TACGCCGCAG CCGATGATGC TACACTTGCC AGCCGACCCC TTAGCGGATC G
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396.

2019.08.08

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