Please review the QC test results indicated by icon below as well as clone information before placing your order.

hmKO-K/pRSETB

Plasmid clone of hmKO-K, faster-maturating version of Kusabira orange.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A. (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A. (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 付加的提供条件： 本件リソースの使用は学術機関での学術研究に限る。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15314_A7Htp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp () Region: insert Sequence file: RDB15314_A7Hta.seq

>D04498J5_A7H9_1_T7_20bp_G08_20_ABI24.ab1     1 GAAATCAGTA GTATAATTTT GTTTACTTTA AGAAGGAGAT ATACATATGC GGGGTTCTCA    61 TCATCATCAT CATCATGGTA TGGCTAGCAT GACTGGTGGA CAGCAAATGG GTCGGGATCT   121 GTACGACGAT GACGATAAGG ATCCGACCAT GGTGAGCGTG ATCAAGCCCG AGATGAAGAT   181 GAGGTACTAC ATGGACGGCT CCGTCAATGG GCATGAGTTC ACAATCGAGG GTGAGGGCAC   241 AGGCAGACCT TACGAGGGAC ATCAGGAGAT GACACTGCGC GTCACAATGG CCGAGGGCGG   301 GCCAATGCCT TTCGCCTTCG ACCTGGTGTC CCACGTGTTC TGTTACGGCC ACAGAGTTTT   361 TACCAAGTAC CCAGAAGAGA TCCCAGACTA TTTCAAGCAG GCCTTTCCTG AGGGCCTGTC   421 CTGGGAGAGG TCCCTGGAGT TCGAGGACGG CGGCTCCGCC TCCGTGAGCG CCCACATCAG   481 CCTGAGGGGC AACACCTTCT ACCACAAGTC CAAGTTCACC GGCGTGAACT TCCCCGCCGA   541 CGGCCCCATC ATGCAGAACC AGAGCGTGGA CTGGGAGCCC TCCACCGAGA AGATCACCGC   601 CAGCGACGGC GTGCTGAAGG GCGACGTGAC CATGTACCTG AAGCTGGAGG GCGGCGGCAA   661 CCACAAGTGC CAGTTCAAGA CCACCTACAA GGCCGCCAAG GAGATCCTGG AGATGCCCGG   721 CGACCACTAC ATCGGCCACA GGCTGGTGAG GAAGACCGAG GGCAACATCA CCGAGCAGGT   781 GGAGGACGCC GTGGCCCACT CCTAAGAATT CGAAGCTTGA TCCGGCTGCT AACAAAGCCC   841 GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG   901 CCTCTAAACG GGTCTTGAGG GGTTTTTTTG CTGAAAGGAG GAACTATATC CGGGATCTGG   961 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTTCCCAA CAGTTTGCGC AGCCCTGAAA  1021 TGGGCGAATG GGACGCGCCC TGTAGCGGCG CATTAGCCGC CGGCCGGGTT GTTGGTTGAT 1081 TACGCCGCAG CCGATGATGC TACACTTGCC AGCCGACCCC TTAGCGGATC G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.17

GNP_filter3_RDBDEP_html_230702.pl