RDB15314 - hmKO-K/pRSETB

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

hmKO-K/pRSETB (#RDB15314)

Plasmid clone of hmKO-K, faster-maturating version of Kusabira orange.

Alternative name	hmKOkappa/pRSETB
Clone info.	Plasmid clone of hmKO-K, faster-maturating version of Kusabira orange.
Vector backbone	pRSET B (Plasmid)
Gene/insert name	Fungia concinna Orange fluorescent protein cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A. (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A. (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A. (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15314	hmKO-K/pRSETB	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The hmKO-K/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15314).

Reference section:

Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15314_A7H9p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp (Pr0261) Region: 6xHis,T7 tag,Xpress™ tag,insert 5' Sequence file: RDB15314_A7Htb.seq
>15314_15314_B4BqF_1_T7_20bp_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNTTT GTTNNNTTTA AGAAGGAGAN ATACATATGC GGGGTTCTCA 61 TCATCATCAT CATCATGGTA TGGCTAGCAT GACTGGTGGA CAGCAAATGG GTCGGGATCT 121 GTACGACGAT GACGATAAGG ATCCGACCAT GGTGAGCGTG ATCAAGCCCG AGATGAAGAT 181 GAGGTACTAC ATGGACGGCT CCGTCAATGG GCATGAGTTC ACAATCGAGG GTGAGGGCAC 241 AGGCAGACCT TACGAGGGAC ATCAGGAGAT GACACTGCGC GTCACAATGG CCGAGGGCGG 301 GCCAATGCCT TTCGCCTTCG ACCTGGTGTC CCACGTGTTC TGTTACGGCC ACAGAGTTTT 361 TACCAAGTAC CCAGAAGAGA TCCCAGACTA TTTCAAGCAG GCCTTTCCTG AGGGCCTGTC 421 CTGGGAGAGG TCCCTGGAGT TCGAGGACGG CGGCTCCGCC TCCGTGAGCG CCCACATCAG 481 CCTGAGGGGC AACACCTTCT ACCACAAGTC CAAGTTCACC GGCGTGAACT TCCCCGCCGA 541 CGGCCCCATC ATGCAGAACC AGAGCGTGGA CTGGGAGCCC TCCACCGAGA AGANCACCGC 601 CAGCGACNGC GTGCTGAAGG GCGACGTGAC CATGTACCTG AAGCTGGAGG GNGGCGGCAA 661 CCACNAGTGN CAGTTCNAGA NCACCTACNN NNNCGNCNNN NNNNNCCTNN NNNNGNCCNG 721 NNNNNNCNAC NTCNGNNNNN NNNGNNNNNN NNNN //
Primer: f1ori_R3 (Pr0142) Region: T7 ter,insert 3' Sequence file: RDB15314_A7Htc.seq
>D04498J5_A7H9_1_f1ori_R3_H08_23_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNTN NNNNNNGNNN NNNNNNNNNN NNNCCTCTTC 61 GCTATTACNN NCNGATCCGG ATATAGTTCC TCCTTTCAGC AAAAAACCCC TCAAGACCCG 121 TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC 181 TTCCTTTCGG GCTTTGTTAG CAGCCGGATC AAGCTTCGAA TTCTTAGGAG TGGGCCACGG 241 CGTCCTCCAC CTGCTCGGTG ATGTTGCCCT CGGTCTTCCT CACCAGCCTG TGGCCGATGT 301 AGTGGTCGCC GGGCATCTCC AGGATCTCCT TGGCGGCCTT GTAGGTGGTC TTGAACTGGC 361 ACTTGTGGTT GCCGCCGCCC TCCAGCTTCA GGTACATGGT CACGTCGCCC TTCAGCACGC 421 CGTCGCTGGC GGTGATCTTC TCGGTGGAGG GCTCCCAGTC CACGCTCTGG TTCTGCATGA 481 TGGGGCCGTC GGCGGGGAAG TTCACGCCGG NNNNNNTGGA CTTGTGGTAG AAGGTGTTGC 541 CCCTCAGGCT GATGTGGGCG CTCACGGAGG CGGAGCCGCC GTCCTCGAAC TCCAGGGACC 601 TCTCCCAGGA CAGGCCCTCA GGAAAGGCCT GCTTGAAATA GTCTGGGATC TCTTCTGGGT 661 ACTTGGTAAA AACTCTGTGG CCGTAACANA ACACGTGGGA CACCANNTCG AAGGNGAAAG 721 GNATTGGCCC GCCCTCGGCC ATTGTGACGC GCAGTGTCAT CTCCTGATGT CCCTCGTAAG 781 GTCTGCCTGT GCCCTCACCC TCGATTGTGA ACTCATGNCC ATTGACGGAN CCNTCCNTGT 841 AGTANCTCAT CTTCATCTNN NNNCTTGATC ACGCTCACCA TGGGTCGGGA TCCTTATCGT 901 CNTCNTCGTA CAGATNCNNN ANCCNTTTNC NGTCCACCAN NCNTGCTANC CNTANCATGN 961 NNGNTNNNGA TGATGANNAN NCCCNNATNN GNNTATNNNC NNNNNNNNGN TNAANNNNNN 1021 NNNTTTCTAN NGNNNAAANN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. [link to RRC of NBRP]

Resource search

Link

Other resources in our bank

External Database
Fungia concinna Orange fluorescent protein

Reference sequence