Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

hmKO-K/pRSETB (#RDB15314)

Plasmid clone of hmKO-K, faster-maturating version of Kusabira orange.

Alternative name hmKOkappa/pRSETB
Clone info. Plasmid clone of hmKO-K, faster-maturating version of Kusabira orange.
Vector backbone pRSET B (Plasmid)
Gene/insert name Fungia concinna Orange fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Tsutsui, H. et al., Nat. Methods 5 (8): 683-685 (2008).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Tsutsui, H. et al., Nat. Methods 5 (8): 683-685 (2008).
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15314 hmKO-K/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The hmKO-K/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15314).

Reference section:

Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15314_A7H9p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp (Pr0261)
Region: 6xHis,T7 tag,Xpress™ tag,insert 5'
Sequence file: RDB15314_A7Htb.seq check
>15314_15314_B4BqF_1_T7_20bp_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNTTT GTTNNNTTTA AGAAGGAGAN ATACATATGC GGGGTTCTCA
   61 TCATCATCAT CATCATGGTA TGGCTAGCAT GACTGGTGGA CAGCAAATGG GTCGGGATCT
  121 GTACGACGAT GACGATAAGG ATCCGACCAT GGTGAGCGTG ATCAAGCCCG AGATGAAGAT
  181 GAGGTACTAC ATGGACGGCT CCGTCAATGG GCATGAGTTC ACAATCGAGG GTGAGGGCAC
  241 AGGCAGACCT TACGAGGGAC ATCAGGAGAT GACACTGCGC GTCACAATGG CCGAGGGCGG
  301 GCCAATGCCT TTCGCCTTCG ACCTGGTGTC CCACGTGTTC TGTTACGGCC ACAGAGTTTT
  361 TACCAAGTAC CCAGAAGAGA TCCCAGACTA TTTCAAGCAG GCCTTTCCTG AGGGCCTGTC
  421 CTGGGAGAGG TCCCTGGAGT TCGAGGACGG CGGCTCCGCC TCCGTGAGCG CCCACATCAG
  481 CCTGAGGGGC AACACCTTCT ACCACAAGTC CAAGTTCACC GGCGTGAACT TCCCCGCCGA
  541 CGGCCCCATC ATGCAGAACC AGAGCGTGGA CTGGGAGCCC TCCACCGAGA AGANCACCGC
  601 CAGCGACNGC GTGCTGAAGG GCGACGTGAC CATGTACCTG AAGCTGGAGG GNGGCGGCAA
  661 CCACNAGTGN CAGTTCNAGA NCACCTACNN NNNCGNCNNN NNNNNCCTNN NNNNGNCCNG
721 NNNNNNCNAC NTCNGNNNNN NNNGNNNNNN NNNN
//
Primer: f1ori_R3 (Pr0142)
Region: T7 ter,insert 3'
Sequence file: RDB15314_A7Htc.seq check
>D04498J5_A7H9_1_f1ori_R3_H08_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNTN NNNNNNGNNN NNNNNNNNNN NNNCCTCTTC
   61 GCTATTACNN NCNGATCCGG ATATAGTTCC TCCTTTCAGC AAAAAACCCC TCAAGACCCG
  121 TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC
  181 TTCCTTTCGG GCTTTGTTAG CAGCCGGATC AAGCTTCGAA TTCTTAGGAG TGGGCCACGG
  241 CGTCCTCCAC CTGCTCGGTG ATGTTGCCCT CGGTCTTCCT CACCAGCCTG TGGCCGATGT
  301 AGTGGTCGCC GGGCATCTCC AGGATCTCCT TGGCGGCCTT GTAGGTGGTC TTGAACTGGC
  361 ACTTGTGGTT GCCGCCGCCC TCCAGCTTCA GGTACATGGT CACGTCGCCC TTCAGCACGC
  421 CGTCGCTGGC GGTGATCTTC TCGGTGGAGG GCTCCCAGTC CACGCTCTGG TTCTGCATGA
  481 TGGGGCCGTC GGCGGGGAAG TTCACGCCGG NNNNNNTGGA CTTGTGGTAG AAGGTGTTGC
  541 CCCTCAGGCT GATGTGGGCG CTCACGGAGG CGGAGCCGCC GTCCTCGAAC TCCAGGGACC
  601 TCTCCCAGGA CAGGCCCTCA GGAAAGGCCT GCTTGAAATA GTCTGGGATC TCTTCTGGGT
  661 ACTTGGTAAA AACTCTGTGG CCGTAACANA ACACGTGGGA CACCANNTCG AAGGNGAAAG
  721 GNATTGGCCC GCCCTCGGCC ATTGTGACGC GCAGTGTCAT CTCCTGATGT CCCTCGTAAG
  781 GTCTGCCTGT GCCCTCACCC TCGATTGTGA ACTCATGNCC ATTGACGGAN CCNTCCNTGT
  841 AGTANCTCAT CTTCATCTNN NNNCTTGATC ACGCTCACCA TGGGTCGGGA TCCTTATCGT
  901 CNTCNTCGTA CAGATNCNNN ANCCNTTTNC NGTCCACCAN NCNTGCTANC CNTANCATGN
  961 NNGNTNNNGA TGATGANNAN NCCCNNATNN GNNTATNNNC NNNNNNNNGN TNAANNNNNN
1021 NNNTTTCTAN NGNNNAAANN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. [link to RRC of NBRP]