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Flamindo (R279E)/pcDNA3

Expression vector of mutant Flamindo (negative control).

Catalog number RDB15248
Resource name Flamindo (R279E)/pcDNA3
Clone info. Expression vector of mutant Flamindo having R279E substitition disrupting the cAMP-binding. A variant of YFP, Citrine was interrupted by cAMP-binding domain of mouse Epac1 (157-316aa). For the compositon, refer Fig.1 of Kitaguchi, T., Biochem. J. 450: 365-373, 2013.
Use this clone as a negative control of Flamindo/pcDNA3 (RDB15247).
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name mouse Epac1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank mouse Rapgef3 (NCBI Gene 223864) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Kitaguchi T, Oya M, Wada Y, Tsuboi T, Miyawaki A. (2013) Extracellular Calcium influx activates Adenylate Cyclase 1 and potentiates Insulin secretion in MIN6 cells. Biochem. J., 450 (2): 365-373.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15248 Flamindo (R279E)/pcDNA3 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kitaguchi, T., Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells. Biochem. J. 450 (2): 365-373 (2013). PMID 23282092.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15248_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15248_A7H9a.seq check
>D04498F6-1_A7H9_CMV-Forward_B09_06_ABI24.ab1
    1 CGTTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG
  181 TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG
  241 AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA
  301 AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCTTCGG CTACGGCCTG ATGTGCTTCG
  361 CCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT
  421 ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG
  481 TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG
  541 AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACCT CCGCGGGGAG GAGCTTGTTG
  601 AGGCTATGGC CCTCCTGTCC CAGCGAGGGC CTGATGCCCT ACTCACCGTG GCGCTCCGGA
  661 AGCCCCCAGG TCAGCGTACG GATGAAGAAC TGGACCTGAT CTTTGAAGAG CTGCTGCATA
  721 TCAAGGCGGT GGCACACCTT TCTAACTCGG TGAAGCGGGA ACTAGCTGCT GTTCTGCTCT
  781 TTGAACCACA CAGCAAGGCA GGAACTGTGT TGTTCAGCCA GGGGGACAAG GGTACCTCGT
  841 GGTACATTAT CTGGAAGGGA TCTGTCAATG TGGTGACCCA TGGCAAGGGG CTGGTGACCA
  901 CGTTGCACGA GGGAGATGAC TTTGGACAGC TGGCTCTGGT GAACGACGCA CCTGAGGCAG
  961 CCACCATCAT CCTTCGAGAA AATAACTGTC ACTTTTCTGC GTGTGGACAA GCAGGACTTC
 1021 AACCCGCATC ATCAAGGATG TGGAAAGCAA AACCCATGAG ACTGGGAAGG AAGAATTCAC
1081 AGCTACACGT CCTTATTATC ATGGCCCGGA ACAAAGTCAG GCAAGGGAC
//
Primer: BGH_rev2
Sequence file: RDB15248_A7H9b.seq check
>D04498F6-1_A7H9_BGH_rev2_C09_09_ABI24.ab1
    1 CAGGCGGCTA GAGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG
  121 CATGCTCGAG TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA
  181 ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGT
  241 AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT
  301 GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA
  361 CCTTGATGCC GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGAATTCTT
  421 CTTCCAGTCT CATGGTTTTT GCTTCCACAT CCTTGATGAT GCGGTTGAAG TCCTGCTTGT
  481 CCACACGCAG AAAGTGACAG TTATTTTCTC GAAGGATGAT GGTGGCTGCC TCAGGTGCGT
  541 CGTTCACCAG AGCCAGCTGT CCAAAGTCAT CTCCCTCGTG CAACGTGGTC ACCAGCCCCT
  601 TGCCATGGGT CACCACATTG ACAGATCCCT TCCAGATAAT GTACCACGAG GTACCCTTGT
  661 CCCCCTGGCT GAACAACACA GTTCCTGCCT TGCTGTGTGG TTCAAAGAGC AGAACAGCAG
  721 CTAGTTCCCG CTTCACCGAG TTAGAAAGGT GTGCCACCGC CTTGATATGC AGCAGCTCTT
  781 CAAAGATCAG GTCCAGTTCT TCATCCGTAC GCTGACCTGG GGGCTTCCGG AGCGCCACGG
  841 TGAGTAGGGC ATCAGGCCCT CGCTGGGACA GGAGGGCCAT AGCCTCAACA AGCTCCTCCC
  901 CGCGGAGGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC
  961 CCTTCAGCTC GATGCGGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC
 1021 TTGTAGTTGC GTCGTCTTGA GAGATGGTGC GGCTCCTGGA CGTAGCCTTT CGGCATGACG
1081 GACTGCAGAA GTCGTGCTGC TTCATGGGTG GATTCGGGGG GGA
//
Primer: SV40pro_F
Sequence file: RDB15248_A7H9c.seq check
>D04498F6-1_A7H9_SV40pro_F_D09_12_ABI24.ab1
    1 CCTGGTTCTG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC
  961 CCAACCTGCC CATCACGAGA TTTCGATTCC ACCGCCCGCC CTTCTATGAA AGGTTGGGCT
 1021 TTCGGAAATC GTTTTCCGGG ACGCCGGCTG GGATGATCCT TCAGCCGCGG GGATCTCATG
1081 CTGAAGTCAT CGACCAACCC GACCTGATTA TTGCAGCTTT TAATAATGGG CT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kitaguchi, T., Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells. Biochem. J. 450 (2): 365-373 (2013). PMID 23282092.

2020.12.17

GNP_filter3_RDBDEP_html_201216.pl