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Flamindo (R279E)/pcDNA3

Expression vector of mutant Flamindo (negative control).

Catalog number RDB15248
Resource name Flamindo (R279E)/pcDNA3
Clone info. Expression vector of mutant Flamindo having R279E substitition disrupting the cAMP-binding. A variant of YFP, Citrine was interrupted by cAMP-binding domain of mouse Epac1 (157-316aa). For the compositon, refer Fig.1 of Kitaguchi, T., Biochem. J. 450: 365-373, 2013.
Comment Use this clone as a negative control of Flamindo/pcDNA3 (RDB15247).
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name mouse Epac1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
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External Database
mouse Epac1 Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Kitaguchi T, Oya M, Wada Y, Tsuboi T, Miyawaki A. (2013) Extracellular Calcium influx activates Adenylate Cyclase 1 and potentiates Insulin secretion in MIN6 cells. Biochem. J., 450 (2): 365-373.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Kitaguchi T, Oya M, Wada Y, Tsuboi T, Miyawaki A. (2013) Extracellular Calcium influx activates Adenylate Cyclase 1 and potentiates Insulin secretion in MIN6 cells. Biochem. J., 450 (2): 365-373.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
          宮脇敦史博士による提供承諾書 [Word]
          備考 提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15248 Flamindo (R279E)/pcDNA3 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kitaguchi, T., Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells. Biochem. J. 450 (2): 365-373 (2013). PMID 23282092.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15248_A7H9p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB15248_A7H9a.seq check
          >D04498F6-1_A7H9_CMV-Forward_B09_06_ABI24.ab1
              1 CGTTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTTATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG
            181 TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG
            241 AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA
            301 AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCTTCGG CTACGGCCTG ATGTGCTTCG
            361 CCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT
            421 ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG
            481 TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG
            541 AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACCT CCGCGGGGAG GAGCTTGTTG
            601 AGGCTATGGC CCTCCTGTCC CAGCGAGGGC CTGATGCCCT ACTCACCGTG GCGCTCCGGA
            661 AGCCCCCAGG TCAGCGTACG GATGAAGAAC TGGACCTGAT CTTTGAAGAG CTGCTGCATA
            721 TCAAGGCGGT GGCACACCTT TCTAACTCGG TGAAGCGGGA ACTAGCTGCT GTTCTGCTCT
            781 TTGAACCACA CAGCAAGGCA GGAACTGTGT TGTTCAGCCA GGGGGACAAG GGTACCTCGT
            841 GGTACATTAT CTGGAAGGGA TCTGTCAATG TGGTGACCCA TGGCAAGGGG CTGGTGACCA
            901 CGTTGCACGA GGGAGATGAC TTTGGACAGC TGGCTCTGGT GAACGACGCA CCTGAGGCAG
            961 CCACCATCAT CCTTCGAGAA AATAACTGTC ACTTTTCTGC GTGTGGACAA GCAGGACTTC
           1021 AACCCGCATC ATCAAGGATG TGGAAAGCAA AACCCATGAG ACTGGGAAGG AAGAATTCAC
          1081 AGCTACACGT CCTTATTATC ATGGCCCGGA ACAAAGTCAG GCAAGGGAC
          //
          Primer: BGH_rev2
          Sequence file: RDB15248_A7H9b.seq check
          >D04498F6-1_A7H9_BGH_rev2_C09_09_ABI24.ab1
              1 CAGGCGGCTA GAGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT
             61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG
            121 CATGCTCGAG TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA
            181 ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGT
            241 AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT
            301 GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA
            361 CCTTGATGCC GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGAATTCTT
            421 CTTCCAGTCT CATGGTTTTT GCTTCCACAT CCTTGATGAT GCGGTTGAAG TCCTGCTTGT
            481 CCACACGCAG AAAGTGACAG TTATTTTCTC GAAGGATGAT GGTGGCTGCC TCAGGTGCGT
            541 CGTTCACCAG AGCCAGCTGT CCAAAGTCAT CTCCCTCGTG CAACGTGGTC ACCAGCCCCT
            601 TGCCATGGGT CACCACATTG ACAGATCCCT TCCAGATAAT GTACCACGAG GTACCCTTGT
            661 CCCCCTGGCT GAACAACACA GTTCCTGCCT TGCTGTGTGG TTCAAAGAGC AGAACAGCAG
            721 CTAGTTCCCG CTTCACCGAG TTAGAAAGGT GTGCCACCGC CTTGATATGC AGCAGCTCTT
            781 CAAAGATCAG GTCCAGTTCT TCATCCGTAC GCTGACCTGG GGGCTTCCGG AGCGCCACGG
            841 TGAGTAGGGC ATCAGGCCCT CGCTGGGACA GGAGGGCCAT AGCCTCAACA AGCTCCTCCC
            901 CGCGGAGGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC
            961 CCTTCAGCTC GATGCGGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC
           1021 TTGTAGTTGC GTCGTCTTGA GAGATGGTGC GGCTCCTGGA CGTAGCCTTT CGGCATGACG
          1081 GACTGCAGAA GTCGTGCTGC TTCATGGGTG GATTCGGGGG GGA
          //
          Primer: SV40pro_F
          Sequence file: RDB15248_A7H9c.seq check
          >D04498F6-1_A7H9_SV40pro_F_D09_12_ABI24.ab1
              1 CCTGGTTCTG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
             61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
            121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
            181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
            241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
            301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
            361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
            421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
            481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
            541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
            601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
            661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
            721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
            781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
            841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
            901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC
            961 CCAACCTGCC CATCACGAGA TTTCGATTCC ACCGCCCGCC CTTCTATGAA AGGTTGGGCT
           1021 TTCGGAAATC GTTTTCCGGG ACGCCGGCTG GGATGATCCT TCAGCCGCGG GGATCTCATG
          1081 CTGAAGTCAT CGACCAACCC GACCTGATTA TTGCAGCTTT TAATAATGGG CT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kitaguchi, T., Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells. Biochem. J. 450 (2): 365-373 (2013). PMID 23282092.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl