RDB15248 - Flamindo (R279E)/pcDNA3

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

Flamindo (R279E)/pcDNA3 (#RDB15248)

Expression vector of mutant Flamindo (negative control).

Clone info.	Expression vector of mutant Flamindo having R279E substitition disrupting the cAMP-binding. A variant of YFP, Citrine was interrupted by cAMP-binding domain of mouse Epac1 (157-316aa). For the compositon, refer Fig.1 of Kitaguchi, T., Biochem. J. 450: 365-373, 2013.
Comment	Use this clone as a negative control of Flamindo/pcDNA3 (RDB15247).
Vector backbone	pcDNA3 (plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	mouse Epac1 cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Kitaguchi T, Oya M, Wada Y, Tsuboi T, Miyawaki A. (2013) Extracellular Calcium influx activates Adenylate Cyclase 1 and potentiates Insulin secretion in MIN6 cells. Biochem. J., 450 (2): 365-373.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kitaguchi T, Oya M, Wada Y, Tsuboi T, Miyawaki A. (2013) Extracellular Calcium influx activates Adenylate Cyclase 1 and potentiates Insulin secretion in MIN6 cells. Biochem. J., 450 (2): 365-373. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kitaguchi T, Oya M, Wada Y, Tsuboi T, Miyawaki A. (2013) Extracellular Calcium influx activates Adenylate Cyclase 1 and potentiates Insulin secretion in MIN6 cells. Biochem. J., 450 (2): 365-373. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15248	Flamindo (R279E)/pcDNA3	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Flamindo (R279E)/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15248).

Reference section:

Kitaguchi, T., Oya, M., Wada, Y., Tsuboi, T., Miyawaki, A., Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells. Biochem. J. 450 (2): 365-373 (2013). PMID 23282092. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15248_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward () Sequence file: RDB15248_A7H9a.seq
>D04498F6-1_A7H9_CMV-Forward_B09_06_ABI24.ab1 1 CGTTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC 121 TCACTATAGG GAGACCCAAG CTTATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG 181 TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG 241 AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA 301 AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCTTCGG CTACGGCCTG ATGTGCTTCG 361 CCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT 421 ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG 481 TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG 541 AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACCT CCGCGGGGAG GAGCTTGTTG 601 AGGCTATGGC CCTCCTGTCC CAGCGAGGGC CTGATGCCCT ACTCACCGTG GCGCTCCGGA 661 AGCCCCCAGG TCAGCGTACG GATGAAGAAC TGGACCTGAT CTTTGAAGAG CTGCTGCATA 721 TCAAGGCGGT GGCACACCTT TCTAACTCGG TGAAGCGGGA ACTAGCTGCT GTTCTGCTCT 781 TTGAACCACA CAGCAAGGCA GGAACTGTGT TGTTCAGCCA GGGGGACAAG GGTACCTCGT 841 GGTACATTAT CTGGAAGGGA TCTGTCAATG TGGTGACCCA TGGCAAGGGG CTGGTGACCA 901 CGTTGCACGA GGGAGATGAC TTTGGACAGC TGGCTCTGGT GAACGACGCA CCTGAGGCAG 961 CCACCATCAT CCTTCGAGAA AATAACTGTC ACTTTTCTGC GTGTGGACAA GCAGGACTTC 1021 AACCCGCATC ATCAAGGATG TGGAAAGCAA AACCCATGAG ACTGGGAAGG AAGAATTCAC 1081 AGCTACACGT CCTTATTATC ATGGCCCGGA ACAAAGTCAG GCAAGGGAC //
Primer: BGH_rev2 () Sequence file: RDB15248_A7H9b.seq
>D04498F6-1_A7H9_BGH_rev2_C09_09_ABI24.ab1 1 CAGGCGGCTA GAGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT 61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG 121 CATGCTCGAG TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA 181 ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGT 241 AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT 301 GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA 361 CCTTGATGCC GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGAATTCTT 421 CTTCCAGTCT CATGGTTTTT GCTTCCACAT CCTTGATGAT GCGGTTGAAG TCCTGCTTGT 481 CCACACGCAG AAAGTGACAG TTATTTTCTC GAAGGATGAT GGTGGCTGCC TCAGGTGCGT 541 CGTTCACCAG AGCCAGCTGT CCAAAGTCAT CTCCCTCGTG CAACGTGGTC ACCAGCCCCT 601 TGCCATGGGT CACCACATTG ACAGATCCCT TCCAGATAAT GTACCACGAG GTACCCTTGT 661 CCCCCTGGCT GAACAACACA GTTCCTGCCT TGCTGTGTGG TTCAAAGAGC AGAACAGCAG 721 CTAGTTCCCG CTTCACCGAG TTAGAAAGGT GTGCCACCGC CTTGATATGC AGCAGCTCTT 781 CAAAGATCAG GTCCAGTTCT TCATCCGTAC GCTGACCTGG GGGCTTCCGG AGCGCCACGG 841 TGAGTAGGGC ATCAGGCCCT CGCTGGGACA GGAGGGCCAT AGCCTCAACA AGCTCCTCCC 901 CGCGGAGGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC 961 CCTTCAGCTC GATGCGGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC 1021 TTGTAGTTGC GTCGTCTTGA GAGATGGTGC GGCTCCTGGA CGTAGCCTTT CGGCATGACG 1081 GACTGCAGAA GTCGTGCTGC TTCATGGGTG GATTCGGGGG GGA //
Primer: SV40pro_F () Sequence file: RDB15248_A7H9c.seq
>D04498F6-1_A7H9_SV40pro_F_D09_12_ABI24.ab1 1 CCTGGTTCTG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT 121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC 181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG 301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG 421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG 481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG 601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC 661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG 721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC 781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC 901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC 961 CCAACCTGCC CATCACGAGA TTTCGATTCC ACCGCCCGCC CTTCTATGAA AGGTTGGGCT 1021 TTCGGAAATC GTTTTCCGGG ACGCCGGCTG GGATGATCCT TCAGCCGCGG GGATCTCATG 1081 CTGAAGTCAT CGACCAACCC GACCTGATTA TTGCAGCTTT TAATAATGGG CT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kitaguchi, T., Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells. Biochem. J. 450 (2): 365-373 (2013). PMID 23282092. [link to RRC of NBRP]

Resource search

Link

Other resources in our bank

mouse Epac1

External Database
mouse Epac1

Aequorea victoria GFP

Reference sequence