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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBS coupler 2

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Catalog number RDB15127
Resource name pBS coupler 2
Clone info. Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15127 pBS coupler 2 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15127_A7H9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13R
            Region: insert, f1 ori
            Sequence file: RDB15127_A7H9a.seq check
            >D04498B3_A7H9_1_M13R_G03_21_ABI24.ab1
                1 ACCGCCCCCA ATTTAACCCT TCACTAAAGG GGAACAAAAG CTGGGGTACC GGGCCCCCCC
               61 TCGAGGTCGA CGGTATCGAT AAGCTTGATG GTGGAGGCGG TTCAGGCGGA GGTGGCTCTG
              121 GCGGTGGCGG ATCGCATCGA ATTCCTGCAG CCCGGGGGAT CCACTAGTTC TAGAGCGGCC
              181 GCCACCGCGG TGGAGCTCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC
              241 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC
              301 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC
              361 CCAACAGTTG CGCAGCCTGA ATGGCGAATG GAAATTGTAA GCGTTAATAT TTTGTTAAAA
              421 TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA
              481 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC
              541 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG
              601 GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT
              661 AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG
              721 GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGGCGCTA GGGCGCTGGC
              781 AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA
              841 GGGCGCGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGG AACCCCTATT TGTTTATTTT
              901 TCTAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA ATGCTTCAAT
              961 AATATTGAAA AAAGGAAGAG TATGAAGTAT TTCAACATTT TCCGGTGTCG CCCTTTATTT
             1021 CCCTTTTTTT GCGGGCATTT TTGCCTTTCC CTGGTTATTT GCCTCAACCC AGAAACGGCT
             1081 GGGTGGAAAG TTAAAAGATG CCTGAAGATC AAGTTGGGTT GCCACGAGGT GGGGTACATC
            1141 GAACCTGGAT CTCAACAGCG GAAGAAATTC CTTGGAGAGA GAGTTTTTTT TCGGC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl