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pBS coupler 2

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Catalog number RDB15127
Resource name pBS coupler 2
Clone info. Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
Vector backbone Plasmid
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Material Transfer Agreement (MTA) [Word]
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Remarks
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15127 pBS coupler 2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15127_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: insert, f1 ori | Primer: M13R | Sequence: RDB15127_A7H9a.seq
>D04498B3_A7H9_1_M13R_G03_21_ABI24.ab1
    1 ACCGCCCCCA ATTTAACCCT TCACTAAAGG GGAACAAAAG CTGGGGTACC GGGCCCCCCC
   61 TCGAGGTCGA CGGTATCGAT AAGCTTGATG GTGGAGGCGG TTCAGGCGGA GGTGGCTCTG
  121 GCGGTGGCGG ATCGCATCGA ATTCCTGCAG CCCGGGGGAT CCACTAGTTC TAGAGCGGCC
  181 GCCACCGCGG TGGAGCTCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC
  241 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC
  301 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC
  361 CCAACAGTTG CGCAGCCTGA ATGGCGAATG GAAATTGTAA GCGTTAATAT TTTGTTAAAA
  421 TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA
  481 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC
  541 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG
  601 GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT
  661 AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG
  721 GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGGCGCTA GGGCGCTGGC
  781 AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA
  841 GGGCGCGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGG AACCCCTATT TGTTTATTTT
  901 TCTAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA ATGCTTCAAT
  961 AATATTGAAA AAAGGAAGAG TATGAAGTAT TTCAACATTT TCCGGTGTCG CCCTTTATTT
 1021 CCCTTTTTTT GCGGGCATTT TTGCCTTTCC CTGGTTATTT GCCTCAACCC AGAAACGGCT
 1081 GGGTGGAAAG TTAAAAGATG CCTGAAGATC AAGTTGGGTT GCCACGAGGT GGGGTACATC
1141 GAACCTGGAT CTCAACAGCG GAAGAAATTC CTTGGAGAGA GAGTTTTTTT TCGGC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471.

2019.11.02

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