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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBS coupler 2 (#RDB15127)

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Clone info. Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S,, Miyawaki, A., Methods Cell Biol. 85C: 381-393 (2008).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S,, Miyawaki, A., Methods Cell Biol. 85C: 381-393 (2008).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15127 pBS coupler 2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBS coupler 2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15127).

Reference section:

Shimozono, S., Miyawaki, A., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15127_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13R (Pr0160)
Region: insert, f1 ori
Sequence file: RDB15127_A7H9a.seq check
>D04498B3_A7H9_1_M13R_G03_21_ABI24.ab1
    1 ACCGCCCCCA ATTTAACCCT TCACTAAAGG GGAACAAAAG CTGGGGTACC GGGCCCCCCC
   61 TCGAGGTCGA CGGTATCGAT AAGCTTGATG GTGGAGGCGG TTCAGGCGGA GGTGGCTCTG
  121 GCGGTGGCGG ATCGCATCGA ATTCCTGCAG CCCGGGGGAT CCACTAGTTC TAGAGCGGCC
  181 GCCACCGCGG TGGAGCTCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC
  241 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC
  301 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC
  361 CCAACAGTTG CGCAGCCTGA ATGGCGAATG GAAATTGTAA GCGTTAATAT TTTGTTAAAA
  421 TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA
  481 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC
  541 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG
  601 GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT
  661 AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG
  721 GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGGCGCTA GGGCGCTGGC
  781 AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA
  841 GGGCGCGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGG AACCCCTATT TGTTTATTTT
  901 TCTAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA ATGCTTCAAT
  961 AATATTGAAA AAAGGAAGAG TATGAAGTAT TTCAACATTT TCCGGTGTCG CCCTTTATTT
 1021 CCCTTTTTTT GCGGGCATTT TTGCCTTTCC CTGGTTATTT GCCTCAACCC AGAAACGGCT
 1081 GGGTGGAAAG TTAAAAGATG CCTGAAGATC AAGTTGGGTT GCCACGAGGT GGGGTACATC
1141 GAACCTGGAT CTCAACAGCG GAAGAAATTC CTTGGAGAGA GAGTTTTTTT TCGGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP]