Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

22G/pRSETB

Catalog number RDB15255
Resource name 22G/pRSETB
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
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            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Ando R, Mizuno H, Miyawaki A. (2004) Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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            Order form [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Ando R, Mizuno H, Miyawaki A. (2004) Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15255 22G/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The 22G/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15255).

            Reference section:

            Ando, R., Mizuno, H., Miyawaki, A., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

            Tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15255_A7H9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp ()
            Region: 6xHis, T7 tag, Xpress tag, insert
            Sequence file: RDB15255_A7H9a.seq check
            >D04498G4-1_A7H9_T7_20bp_G11_20_ABI24.ab1
                1 GGAATTCGCT GGATATTTTG TTTACTTTAA GAAGGAGATA TACATATGCG GGGTTCTCAT
               61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG
              121 TACGACGATG ACGATAAGGA TCCGATGAGT GTGATTAAAC CAGACATGAA GATCAAGCTG
              181 CGTATGGAAG GCGCTGTAAA TGGACACCCG TTCGCGATTG AAGGAGTTGG CCTTGGGAAG
              241 CCTTTCGAGG GAAAACAGAG TATGGACCTT AAAGTCAAAG AAGGCGGACC TCTGCCTTTC
              301 GCCTATGACA TCTTGACAAC AGTGTTCTGT TACGGCAACA GGGTATTCGC CAAATACCCA
              361 GAAAATATAG TAGACTATTT CAAGCAGTCG TTTCCTGAGG GCTACTCTTG GGAACGAAGC
              421 ATGAATTACG AAGACGGGGG CATTTGTATC GCGACAAACG ACATAACCCT GGATGGTGAC
              481 TGTTTTATCT ATGAAATTCG ATTTGATGGT GTGAACTTTC CTGCCAATGG TCCAGTTATG
              541 CAGAAGAGGA CTGTGAAATG GGAGCCATCC ACTGAGAAAT TGTATGTGCG TGATGGAGTG
              601 CTGAAGGGTG ATGTTAACAT GGCTCTGTTG CTTGAAGGAG GTGGCCATTA CCGATGTGAC
              661 TTCAAAACTA CTTATAAAGC TAAGAAGGTT GTCCAGTTGC CAGACTATCA CTTTGTGGAC
              721 CACCGCATTG AGATTAAAAG CCACGACAAA GATTACAATA ATGTTAATCT GCATGAGCAT
              781 GCCGAAGCGC ATTCTGGGCT GCCGAGGCAG GCCAAGTAAG AATTCGAAGC TTGATCCGGC
              841 TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC
              901 ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT
              961 ATCCGGATCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTTCCC AACAGTTGCG
             1021 CAGCCTGATG GCGAATGGGA CGCGCCCCTG GTAGCCGCCG CAATAGCCGC CGACGGGGTG
            1081 TGTGATTACG CGCAGCCGTG AACGCTAACA CTTGGCCAGG CGACC
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Ando, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. [link to RRC of NBRP]

            2023.08.17

            GNP_filter3_RDBDEP_html_230702.pl