Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

22G/pRSETB (#RDB15255)

Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Ando, R. et al., Science 306 (5700): 1370-1373 (2004).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Ando, R. et al., Science 306 (5700): 1370-1373 (2004).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15255 22G/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The 22G/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15255).

Reference section:

Ando, R., Mizuno, H., Miyawaki, A., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15255_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp ()
Region: 6xHis, T7 tag, Xpress tag, insert
Sequence file: RDB15255_A7H9a.seq check
>D04498G4-1_A7H9_T7_20bp_G11_20_ABI24.ab1
    1 GGAATTCGCT GGATATTTTG TTTACTTTAA GAAGGAGATA TACATATGCG GGGTTCTCAT
   61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG
  121 TACGACGATG ACGATAAGGA TCCGATGAGT GTGATTAAAC CAGACATGAA GATCAAGCTG
  181 CGTATGGAAG GCGCTGTAAA TGGACACCCG TTCGCGATTG AAGGAGTTGG CCTTGGGAAG
  241 CCTTTCGAGG GAAAACAGAG TATGGACCTT AAAGTCAAAG AAGGCGGACC TCTGCCTTTC
  301 GCCTATGACA TCTTGACAAC AGTGTTCTGT TACGGCAACA GGGTATTCGC CAAATACCCA
  361 GAAAATATAG TAGACTATTT CAAGCAGTCG TTTCCTGAGG GCTACTCTTG GGAACGAAGC
  421 ATGAATTACG AAGACGGGGG CATTTGTATC GCGACAAACG ACATAACCCT GGATGGTGAC
  481 TGTTTTATCT ATGAAATTCG ATTTGATGGT GTGAACTTTC CTGCCAATGG TCCAGTTATG
  541 CAGAAGAGGA CTGTGAAATG GGAGCCATCC ACTGAGAAAT TGTATGTGCG TGATGGAGTG
  601 CTGAAGGGTG ATGTTAACAT GGCTCTGTTG CTTGAAGGAG GTGGCCATTA CCGATGTGAC
  661 TTCAAAACTA CTTATAAAGC TAAGAAGGTT GTCCAGTTGC CAGACTATCA CTTTGTGGAC
  721 CACCGCATTG AGATTAAAAG CCACGACAAA GATTACAATA ATGTTAATCT GCATGAGCAT
  781 GCCGAAGCGC ATTCTGGGCT GCCGAGGCAG GCCAAGTAAG AATTCGAAGC TTGATCCGGC
  841 TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC
  901 ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT
  961 ATCCGGATCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTTCCC AACAGTTGCG
 1021 CAGCCTGATG GCGAATGGGA CGCGCCCCTG GTAGCCGCCG CAATAGCCGC CGACGGGGTG
1081 TGTGATTACG CGCAGCCGTG AACGCTAACA CTTGGCCAGG CGACC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ando, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. [link to RRC of NBRP]