Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

22G/pRSETB

Catalog number RDB15255
Resource name 22G/pRSETB
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando R, Mizuno H, Miyawaki A. (2004) Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15255 22G/pRSETB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ando, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15255_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Region: 6xHis, T7 tag, Xpress tag, insert
Sequence file: RDB15255_A7H9a.seq check
>D04498G4-1_A7H9_T7_20bp_G11_20_ABI24.ab1
    1 GGAATTCGCT GGATATTTTG TTTACTTTAA GAAGGAGATA TACATATGCG GGGTTCTCAT
   61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG
  121 TACGACGATG ACGATAAGGA TCCGATGAGT GTGATTAAAC CAGACATGAA GATCAAGCTG
  181 CGTATGGAAG GCGCTGTAAA TGGACACCCG TTCGCGATTG AAGGAGTTGG CCTTGGGAAG
  241 CCTTTCGAGG GAAAACAGAG TATGGACCTT AAAGTCAAAG AAGGCGGACC TCTGCCTTTC
  301 GCCTATGACA TCTTGACAAC AGTGTTCTGT TACGGCAACA GGGTATTCGC CAAATACCCA
  361 GAAAATATAG TAGACTATTT CAAGCAGTCG TTTCCTGAGG GCTACTCTTG GGAACGAAGC
  421 ATGAATTACG AAGACGGGGG CATTTGTATC GCGACAAACG ACATAACCCT GGATGGTGAC
  481 TGTTTTATCT ATGAAATTCG ATTTGATGGT GTGAACTTTC CTGCCAATGG TCCAGTTATG
  541 CAGAAGAGGA CTGTGAAATG GGAGCCATCC ACTGAGAAAT TGTATGTGCG TGATGGAGTG
  601 CTGAAGGGTG ATGTTAACAT GGCTCTGTTG CTTGAAGGAG GTGGCCATTA CCGATGTGAC
  661 TTCAAAACTA CTTATAAAGC TAAGAAGGTT GTCCAGTTGC CAGACTATCA CTTTGTGGAC
  721 CACCGCATTG AGATTAAAAG CCACGACAAA GATTACAATA ATGTTAATCT GCATGAGCAT
  781 GCCGAAGCGC ATTCTGGGCT GCCGAGGCAG GCCAAGTAAG AATTCGAAGC TTGATCCGGC
  841 TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC
  901 ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT
  961 ATCCGGATCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTTCCC AACAGTTGCG
 1021 CAGCCTGATG GCGAATGGGA CGCGCCCCTG GTAGCCGCCG CAATAGCCGC CGACGGGGTG
1081 TGTGATTACG CGCAGCCGTG AACGCTAACA CTTGGCCAGG CGACC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ando, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670.

2020.12.17

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