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mK-GO

Protein-based fluorescent timer, monomeric Kusabira Green Orange (mK-GO).

Catalog number RDB15244
Resource name mK-GO
Clone info. Protein-based fluorescent timer, monomeric Kusabira Green Orange (mK-GO). Six mutations (K49E, P70V, K185E, K188E, S192D, and S196G) were introduced into mKO.
Vector backbone pRSETB (Plasmid)
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15244 mK-GO DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15244_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15244_A7H9a.seq
>D04498F2-2_A7H9_T7_20bp_B08_05_ABI24.ab1
    1 GAAATTCGCT GGAATAATTT TGTTTACTTT AAGAAGGAGA TATACATATG CGGGGTTCTC
   61 ATCATCATCA TCATCATGGT ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGGGATC
  121 TGTACGACGA TGACGATAAG GATCCGACCA TGGTGAGTGT GATTAAACCA GAGATGAAGA
  181 TGAGGTACTA CATGGACGGC TCCGTCAATG GGCATGAGTT CACAATTGAA GGTGAAGGCA
  241 CAGGCAGACC TTACGAGGGA CATCAAGAGA TGACACTACG CGTCACAATG GCCGAGGGCG
  301 GGCCAATGCC TTTCGCGTTT GACTTAGTGT CACACGTGTT CTGTTACGGC CACAGAGTAT
  361 TTACTAAATA TCCAGAAGAG ATACCAGACT ATTTCAAACA AGCATTTCCT GAAGGCCTGT
  421 CATGGGAAAG GTCGTTGGAG TTCGAAGATG GTGGGTCCGC TTCAGTCAGT GCGCATATAA
  481 GCCTTAGAGG AAACACCTTC TACCACAAAT CCAAATTTAC TGGGGTTAAC TTTCCTGCCG
  541 ATGGTCCTAT CATGCAAAAC CAAAGTGTTG ATTGGGAGCC ATCAACCGAG AAAATTACTG
  601 CCAGCGACGG AGTTCTGAAG GGTGATGTTA CGATGTACCT AAAACTTGAA GGAGGCGGCA
  661 ATCACAAATG CCAATTCAAG ACTACTTACA AGGCGGCAAA AGAGATTCTT GAAATGCCAG
  721 GAGACCATTA CATCGGCCAT CGCCTCGTCA GGAAAACCGA AGGCAACATT ACTGAGCTGG
  781 TAGAAGATGC AGTAGCTCAT TCCTAAGAAT TCGAAGCTTG ATCCGGCTGC TAACAAAGCC
  841 CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
  901 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTT GCTGAAAGGA GGAACTATAT CCGGGATCTG
  961 GGCGTAATAG CGAAAGAGGC CCGCACCGAT CGCCCTTTCC CAAACAGTTT GCGCAGCCCT
 1021 GAATGGCGAA ATGGGGACGC GCCCTGTAGC GGGCGGCATT AAGCGCCGGC GGGGTGTGGT
 1081 GGGTTTACGC CGCAGCCGTT GACGCTACAC TTGCCCAGCG CCCCTAGCGG CCCGACCATC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833.

2019.07.30

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