Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mK-GO (#RDB15244)

Protein-based fluorescent timer, monomeric Kusabira Green Orange (mK-GO).

Clone info. Protein-based fluorescent timer, monomeric Kusabira Green Orange (mK-GO). Six mutations (K49E, P70V, K185E, K188E, S192D, and S196G) were introduced into mKO.
Vector backbone pRSETB (Plasmid)
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Tsuboi, T. et al., Mol. Biol. Cell 21 (1): 87-94 (2010).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Tsuboi, T. et al., Mol. Biol. Cell 21 (1): 87-94 (2010).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15244 mK-GO DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mK-GO was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15244).

Reference section:

Tsuboi, T., Kitaguchi, T., Karasawa, S., Fukuda, M., Miyawaki, A., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15244_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp (Pr0261)
Region: 6xHis,T7 tag,Xpress tag,insert 5'
Sequence file: RDB15244_A7H9b.seq check
>D04498F2-2_A7H9_T7_20bp_B08_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNTNNNTTN NNGAAGGANN NATACATATG CGGGGTTCTC
   61 ATCATCATCA TCATCATGGT ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGGGATC
  121 TGTACGACGA TGACGATAAG GATCCGACCA TGGTGAGTGT GATTAAACCA GAGATGAAGA
  181 TGAGGTACTA CATGGACGGC TCCGTCAATG GGCATGAGTT CACAATTGAA GGTGAAGGCA
  241 CAGGCAGACC TTACGAGGGA CATCAAGAGA TGACACTACG CGTCACAATG GCCGAGGGCG
  301 GGCCAATGCC TTTCGCGTTT GACTTAGTGT CACACGTGTT CTGTTACGGC CACAGAGTAT
  361 TTACTAAATA TCCAGAAGAG ATACCAGACT ATTTCAAACA AGCATTTCCT GAAGGCCTGT
  421 CATGGGAAAG GTCGTTGGAG TTCGAAGATG GTGGGTCCGC TTCAGTCAGT GCGCATATAA
  481 GCCTTAGAGG AAACACCTTC TACCACAAAT CCAAATTTAC TGGGGTTAAC TTTCCTGCCG
  541 ATGGTCCTAT CATGCAAAAC CAAAGTGTTG ATTGGGAGCC ATCAACCGAG AAAATTACTG
  601 CCAGCGACGG AGTTCTGAAG GGTGATGTTA CGATGTACCT AAAACTTGAA GGAGGCGGCA
  661 ATCACAAATG CCAATTCAAG ACTACTTACA AGGCGGCAAA AGAGANTCTT GAAATGCCAG
  721 GAGACCATTA CATCGGCCAT CGCCTCGTCA GGAAAACCGA ANGCAACATT ACTGAGCTGG
  781 TAGAAGANGC AGTAGCTCAT TCCTAANAAT TCGAAGCTTG ATCCGGCTGC TAACAAAGCC
  841 CGAAAGGAAN CTGANTTGGC TGCTGCCACC GCTGANCAAT AACTAGCATA ACCCCNNNGG
  901 GCCNNNAAAC GGGNCTNGAG GGGNTTTTTN GCTGAAAGGA NGAACNNTNT CCNGNNTNNN
  961 GNCGNNNNNN CGNANNNNGN CNNCANNNNT NGNCNNTNCC NNNNNNNNTN NNNNNNNCNN
1021 NNNNNNNN
//
Primer: f1ori_R3 (Pr0142)
Region: T7 ter,insert 3'
Sequence file: RDB15244_A7H9c.seq check
>D04498F2-2_A7H9_f1ori_R3_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNNCNNANN GTNGGGNNGG CGNNCGGTGC GGGCCTCTTC GCTATTACGC
   61 CAGATCCGGA TATAGTTCCT CCTTTCAGCA AAAAACCCCT CAAGACCCGT TTAGAGGCCC
  121 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG
  181 CTTTGTTAGC AGCCGGATCA AGCTTCGAAT TCTTAGGAAT GAGCTACTGC ATCTTCTACC
  241 AGCTCAGTAA TGTTGCCTTC GGTTTTCCTG ACGAGGCGAT GGCCGATGTA ATGGTCTCCT
  301 GGCATTTCAA GAATCTCTTT TGCCGCCTTG TAAGTAGTCT TGAATTGGCA TTTGTGATTG
  361 CCGCCTCCTT CAAGTTTTAG GTACATCGTA ACATCACCCT TCAGAACTCC GTCGCTGGCA
  421 GTAATTTTCT CGGTTGATGG CTCCCAATCA ACACTTTGGT TTTGCATGAT AGGACCATCG
  481 GCAGGAAAGT TAACCCCAGT AAATTTGGAT TTGTGGTAGA AGGTGTTTCC TCTAAGGCTT
  541 ATATGCGCAC TGACTGAAGC GGACCCACCA TCTTCGAACT CCAACGACCT TTCCCATGAC
  601 AGGCCTTCAG GAAATGCTTG TTTGAAATAG TCTGGTATCT CTTCTGGATA TTTAGTAAAT
  661 ACTCTGTGGC CGTAACAGAA CACGTGTGAC ACTAAGTCAA ACGCGAAAGG CATTGGCCCG
  721 CCCTCGGCCA TTGTGACGCG TAGTGTCATC TCTTGATGTC CCTCGTAAGG TCTGCCTGTG
  781 CCTTCACCTT CAATTGTGAA CTCATGCCCA TTGACGGANC CGTCCATGTA GTACCTCATC
  841 TTCATCTCTG GTTTAATCAC ACTCACCATG GNCGGNTNCT TATCGTCATC GTCGTNNANA
  901 TCCCNANNCN TTNNCTGNCC ACNANTCATG CNANNNNTAC CNNGNTGATN ATNANGNNGA
961 NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833. [link to RRC of NBRP]