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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mK-GO

Protein-based fluorescent timer, monomeric Kusabira Green Orange (mK-GO).

Catalog number RDB15244
Resource name mK-GO
Clone info. Protein-based fluorescent timer, monomeric Kusabira Green Orange (mK-GO). Six mutations (K49E, P70V, K185E, K188E, S192D, and S196G) were introduced into mKO.
Vector backbone pRSETB (Plasmid)
Depositor|Developer Miyawaki, Atsushi |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15244 mK-GO DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15244_A7H9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp
            Region: 6xHis,T7 tag,Xpress tag,insert 5'
            Sequence file: RDB15244_A7H9b.seq check
            >D04498F2-2_A7H9_T7_20bp_B08_05_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGNTNNNTTN NNGAAGGANN NATACATATG CGGGGTTCTC
               61 ATCATCATCA TCATCATGGT ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGGGATC
              121 TGTACGACGA TGACGATAAG GATCCGACCA TGGTGAGTGT GATTAAACCA GAGATGAAGA
              181 TGAGGTACTA CATGGACGGC TCCGTCAATG GGCATGAGTT CACAATTGAA GGTGAAGGCA
              241 CAGGCAGACC TTACGAGGGA CATCAAGAGA TGACACTACG CGTCACAATG GCCGAGGGCG
              301 GGCCAATGCC TTTCGCGTTT GACTTAGTGT CACACGTGTT CTGTTACGGC CACAGAGTAT
              361 TTACTAAATA TCCAGAAGAG ATACCAGACT ATTTCAAACA AGCATTTCCT GAAGGCCTGT
              421 CATGGGAAAG GTCGTTGGAG TTCGAAGATG GTGGGTCCGC TTCAGTCAGT GCGCATATAA
              481 GCCTTAGAGG AAACACCTTC TACCACAAAT CCAAATTTAC TGGGGTTAAC TTTCCTGCCG
              541 ATGGTCCTAT CATGCAAAAC CAAAGTGTTG ATTGGGAGCC ATCAACCGAG AAAATTACTG
              601 CCAGCGACGG AGTTCTGAAG GGTGATGTTA CGATGTACCT AAAACTTGAA GGAGGCGGCA
              661 ATCACAAATG CCAATTCAAG ACTACTTACA AGGCGGCAAA AGAGANTCTT GAAATGCCAG
              721 GAGACCATTA CATCGGCCAT CGCCTCGTCA GGAAAACCGA ANGCAACATT ACTGAGCTGG
              781 TAGAAGANGC AGTAGCTCAT TCCTAANAAT TCGAAGCTTG ATCCGGCTGC TAACAAAGCC
              841 CGAAAGGAAN CTGANTTGGC TGCTGCCACC GCTGANCAAT AACTAGCATA ACCCCNNNGG
              901 GCCNNNAAAC GGGNCTNGAG GGGNTTTTTN GCTGAAAGGA NGAACNNTNT CCNGNNTNNN
              961 GNCGNNNNNN CGNANNNNGN CNNCANNNNT NGNCNNTNCC NNNNNNNNTN NNNNNNNCNN
            1021 NNNNNNNN
            //
            Primer: f1ori_R3
            Region: T7 ter,insert 3'
            Sequence file: RDB15244_A7H9c.seq check
            >D04498F2-2_A7H9_f1ori_R3_C08_08_ABI24.ab1
                1 NNNNNNNNNN NNNNCNNANN GTNGGGNNGG CGNNCGGTGC GGGCCTCTTC GCTATTACGC
               61 CAGATCCGGA TATAGTTCCT CCTTTCAGCA AAAAACCCCT CAAGACCCGT TTAGAGGCCC
              121 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG
              181 CTTTGTTAGC AGCCGGATCA AGCTTCGAAT TCTTAGGAAT GAGCTACTGC ATCTTCTACC
              241 AGCTCAGTAA TGTTGCCTTC GGTTTTCCTG ACGAGGCGAT GGCCGATGTA ATGGTCTCCT
              301 GGCATTTCAA GAATCTCTTT TGCCGCCTTG TAAGTAGTCT TGAATTGGCA TTTGTGATTG
              361 CCGCCTCCTT CAAGTTTTAG GTACATCGTA ACATCACCCT TCAGAACTCC GTCGCTGGCA
              421 GTAATTTTCT CGGTTGATGG CTCCCAATCA ACACTTTGGT TTTGCATGAT AGGACCATCG
              481 GCAGGAAAGT TAACCCCAGT AAATTTGGAT TTGTGGTAGA AGGTGTTTCC TCTAAGGCTT
              541 ATATGCGCAC TGACTGAAGC GGACCCACCA TCTTCGAACT CCAACGACCT TTCCCATGAC
              601 AGGCCTTCAG GAAATGCTTG TTTGAAATAG TCTGGTATCT CTTCTGGATA TTTAGTAAAT
              661 ACTCTGTGGC CGTAACAGAA CACGTGTGAC ACTAAGTCAA ACGCGAAAGG CATTGGCCCG
              721 CCCTCGGCCA TTGTGACGCG TAGTGTCATC TCTTGATGTC CCTCGTAAGG TCTGCCTGTG
              781 CCTTCACCTT CAATTGTGAA CTCATGCCCA TTGACGGANC CGTCCATGTA GTACCTCATC
              841 TTCATCTCTG GTTTAATCAC ACTCACCATG GNCGGNTNCT TATCGTCATC GTCGTNNANA
              901 TCCCNANNCN TTNNCTGNCC ACNANTCATG CNANNNNTAC CNNGNTGATN ATNANGNNGA
            961 NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833.

            2022.05.16

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