Resource data sheet
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tFucci(SA)2.2/pCSII-CMV

Lentivirus expression vector of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). CMV promoter.

Catalog number RDB15451
Resource name tFucci(SA)2.2/pCSII-CMV
Clone info. Lentivirus expression vector of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). CMV promoter.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence LC192888 (DDBJ accession number)
Originator Miyawaki, Atsushi |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2017.11.09

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