Resource data sheet
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tFucci(SA)2.2/pCSII-CMV

Lentivirus vector construct of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). CMV promoter.

Catalog number RDB15451
Resource name tFucci(SA)2.2/pCSII-CMV
Clone info. Lentivirus expression vector of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence LC192888 (DDBJ accession number)
Originator Miyawaki, Atsushi |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15451_A7Jvp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_R RDB15451_A7Jva.seq
>D05018B2_1_A7Jv_mCherry-C_R_B02_05_ABI24.ab1
    1 GGTGGGAGTA GTCACTTGAT GTTGACGTTG TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG
   61 GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC
  121 TGCTTGATCT CGCCCTTCAG GGCGCCGTCC TCGGGGTACA TCCGCTCGGA GGAGGCCTCC
  181 CAGCCCATGG TCTTCTTCTG CATTACGGGG CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC
  241 TTCACCTTGT AGATGAACTC GCCGTCCTGC AGGGAGGAGT CCTGGGTCAC GGTCACCACG
  301 CCGCCGTCCT CGAAGTTCAT CACGCGCTCC CACTTGAAGC CCTCGGGGAA GGACAGCTTC
  361 AAGTAGTCGG GGATGTCGGC GGGGTGCTTC ACGTAGGCCT TGGAGCCGTA CATGAACTGA
  421 GGGGACAGGA TGTCCCAGGC GAAGGGCAGG GGGCCACCCT TGGTCACCTT CAGCTTGGCG
  481 GTCTGGGTGC CCTCGTAGGG GCGGCCCTCG CCCTCGCCCT CGATCTCGAA CTCGTGGCCG
  541 TTCACGGAGC CCTCCATGTG CACCTTGAAG CGCATGAACT CCTTGATGAT GGCCATGTTA
  601 TCCTCCTCGC CCTTGCTCAC CATGGTGGAT CCTATGCTAG CGGATCTGAC GGTTCACTAA
  661 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG
  721 GGCGGAGTTG TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC
  781 ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC
  841 ATTGATGTAC TGCCAAAACC GCATCACCAT GGGTAATAGC GATGACTAAT ACGTAGATGT
  901 ACTGCCCAAG TAGGAAAGTC CCATACTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT
  961 ACCGTCATTG ACGTCAATAG GGGCGTACTG GCATATGAAT ACACTGAATG TACTGGCTAG
 1021 TGGCAGTTAA CGTTAATACT CCAATCCATT GACGTCATGG AAAGTTCCTT ATTTGGCCGT
1081 AC
//
Primer: mCherry-C_F RDB15451_A7Jvb.seq
>D05018B2_1_A7Jv_mCherry-C_F_C01_03_ABI08.ab1
    1 GACAAGAAAG CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC
   61 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC
  121 ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC GCTCGAGCCC
  181 AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA CCAGTGGCAG CCGCAAGCGC
  241 GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CAGGCCAGGC CACCGGCCCG CAGGAGACTG
  301 CGGCTGTCGG TGGACGAGGT TTCCAGCCCC AGTACCCCCG AGGCCCCAGA CATCCCAGCC
  361 TGCCCTTCTC CGGGCCAGAA GATAAAGAAA TCCACCCCGG CAGCAGGTCA GCCGCCCCAC
  421 CTGACATCCG CGCAGGACCA GGACACCATC ACCGGTCGCG CCAAGCGCGG CAGCGGCGCC
  481 ACCAACTTCA GCCTGCTGAA GCAGGCCGGC GACGTGGAGG AGAACCCCGG CCCCCGCGAA
  541 TTCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG
  601 GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC
  661 TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC
  721 ACCCTCGTGA CCACCCTGAG CTGGGGCGTG CAGTGCTTCG CCCGCTACCC CGACCACATG
  781 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGTACCATC
  841 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC
  901 CTGGTGAACC GCATCGAGCT GAATGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG
  961 GCACAAGCTG GAGTACAACT ACATCAGCGA CAACGTCTAT ATCACCGCCG ACAAGCAGAA
 1021 GAACGGCATC AAGGCCAACT TCAAGATCCG CCACACCATC GAGACCGCGG GCGTGCCAGC
 1081 TCGGCGAACA ACTAACAGGC AGACATCGCC CATTCGGTCG AACTGCTCGA TGCTGCTTGA
1141 CCTGGTAGAC
//
Primer: PRE_rev RDB15451_A7Jvc.seq
>D05018B2_1_A7Jv_PRE_rev_D08_11_ABI24.ab1
    1 CGCTTCCCCA ATAGCGTAAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA
   61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
  121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT
  181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT
  241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC
  301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG
  361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT
  421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC
  481 CCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA
  541 CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGCTT GGACTGGGTG CTCAGGTAGT
  601 GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG
  661 CGAGCTGCAC GCCGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT
  721 TCTTCTGCTT GTCGGCGGTG ATATAGACGT TGTCGCTGAT GTAGTTGTAC TCCAGCTTGT
  781 GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA
  841 GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA
  901 AGATGGTACG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT
  961 TCATGTGGTC GGGGTAGCGG GCGAAGCACT GCACGCCCCA GCTCAGGTTG GTCACGAGGG
 1021 TGGGCCCAGG GCACGGCAGC TTGCCGTGCT GCAGATGGAC CTTCCAGGAT CAGCTTGGCC
 1081 CGTAAGTGCA ATCGATCCGT CGTCCTCGCT GGACACGCCT GGATCTGTAC CGATTACGAT
1141 CGATG
//
Primer: HIVgp_R1 RDB15451_A7Jvd.seq
>D5018B2_1_A7Jv_HIVgp_R1_B01_02_ABI08.ab1
    1 CGGAATCGTA TTCCACTGCT CTTTTTTCTC TCTGCACCAC TCTTCTCTTT GCCTTGGTGG
   61 GTGCTACTCC TAATGGTTCA ATTTTTACTA CTTTATATTT ATATAATTCA CTTCTCCAAT
  121 TGTCCCTCAT ATCTCCTCCT CCAGGTCTGA AGATCAGCGG CCGGCCGCTT GCTGTGCGGT
  181 GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG TGTCTTTTAT
  241 CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG ATCTAAGTTC
  301 TTCTGATCCT GTCTGAAGGG ATGGTTGTAG CTGTCCCAGT ATTTGTCTAC AGCCTTCTGA
  361 TGTTTCTAAC AGGCCAGGAT TAACTGCGAA TCGTTCTAGC TCCCTGCTTG CCCATACTAT
  421 ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CGAATTTTTT CCCATCGCGA
  481 TCTAATTCTC CCCCGCTTAA TACTGACGCT CTCGCACCCA TCTCTCTCCT TCTAGCCTCC
  541 GCTAGTCAAA ATTTTTGGCG TACTCACCAG TCGCCGCCCC TCGCCTCTTG CCGTGCGCGC
  601 TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGT TTCCCTTTCG CTTTCAAGTC
  661 CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC
  721 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT
  781 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG
  841 TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT CCCACCGTAC
  901 ACGCCTACCG CCCATTTGCG TCAATGGGGG CGGAGTTGTT ACGACATTTT TGGAAAGTCC
  961 CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGGTGGA GACTTGGAAA
 1021 TCCCCGTGAG TCAAACCCGC TATCCACGCC CATTGATGTA CTGCCCAAAA CGCATCACAT
 1081 GGTAATAGCG ATGACTATAC GTAGATGTAC CTGGCCCAAG CTAGGAAGGT TCCCCAATAA
1141 GGTTCAATGG
//
Primer: BGH_rev2 RDB15451_A7Jve.seq
>D5018B2_1_A7Jv_BGH_rev2_C01_03_ABI08.ab1
    1 CATTGAAGGG GTAGGAGGCA CGGGGGAGGG GCAACAACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
  121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
  181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
  241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG
  301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG
  361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC
  421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG
  481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG
  541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
  601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
  661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
  721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
  781 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG
  841 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG
  901 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA
  961 GGCGAGCAGC CATGGAAAGG GACGTCAGCT TCCCCGACAA CACCACGGGA ATTGTCAGTG
 1021 CCCACAGCCC GAGCCCCTGT CAGCAGCGGC AGGCAGGCGG CGAATGAGTC GCGGTGTCAA
1081 TAGGAAGGGG AAGCGAAGTC CCGGAAAGGA GCTGACAGGA TGAGTTGCCA
//
Primer: SV40pro_F RDB15451_A7Jvf.seq
>D05018B2_1_A7Jv_SVpro40_F_B01_02_ABI08.ab1
    1 CCCTGGTCCC GTGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
  121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
  181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT
  241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT
  301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC
  361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT
  421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG
  481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA
  541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
  601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
  661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
  721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
  781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC
  841 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
  901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
  961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
 1021 TTAATGAAAT CGGCCAACGC GCGGGGAGGA GGCGGTTTTG CGTATGGGCG CTCTTCGGCA
 1081 TCTTCGCTCA CTGACTCGCT GCGCTCGGAT CGGTCGCTGC GCGAGTGGTA ATCTCCAGAG
1141 C
//

Please visit Sequencing and PCR primers for primer information.

References and tips

Electronic file

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2018.03.15

GNP_filter3_RDBDEP_html_171214.pl