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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(SA)2.2/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). CMV promoter.

Catalog number RDB15451
Resource name tFucci(SA)2.2/pCSII-CMV
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). CMV promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of insert sequence LC192888 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Aequorea victoria GFP

        Reference sequence
          
        Lentiviral plasmid list. [link]

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Ordering instruction.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15451 tFucci(SA)2.2/pCSII-CMV DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15451_A7Jvp1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: mCherry-C_R
        Sequence file: RDB15451_A7Jva.seq check
        >D05018B2_1_A7Jv_mCherry-C_R_B02_05_ABI24.ab1
            1 GGTGGGAGTA GTCACTTGAT GTTGACGTTG TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG
           61 GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC
          121 TGCTTGATCT CGCCCTTCAG GGCGCCGTCC TCGGGGTACA TCCGCTCGGA GGAGGCCTCC
          181 CAGCCCATGG TCTTCTTCTG CATTACGGGG CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC
          241 TTCACCTTGT AGATGAACTC GCCGTCCTGC AGGGAGGAGT CCTGGGTCAC GGTCACCACG
          301 CCGCCGTCCT CGAAGTTCAT CACGCGCTCC CACTTGAAGC CCTCGGGGAA GGACAGCTTC
          361 AAGTAGTCGG GGATGTCGGC GGGGTGCTTC ACGTAGGCCT TGGAGCCGTA CATGAACTGA
          421 GGGGACAGGA TGTCCCAGGC GAAGGGCAGG GGGCCACCCT TGGTCACCTT CAGCTTGGCG
          481 GTCTGGGTGC CCTCGTAGGG GCGGCCCTCG CCCTCGCCCT CGATCTCGAA CTCGTGGCCG
          541 TTCACGGAGC CCTCCATGTG CACCTTGAAG CGCATGAACT CCTTGATGAT GGCCATGTTA
          601 TCCTCCTCGC CCTTGCTCAC CATGGTGGAT CCTATGCTAG CGGATCTGAC GGTTCACTAA
          661 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG
          721 GGCGGAGTTG TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC
          781 ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC
          841 ATTGATGTAC TGCCAAAACC GCATCACCAT GGGTAATAGC GATGACTAAT ACGTAGATGT
          901 ACTGCCCAAG TAGGAAAGTC CCATACTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT
          961 ACCGTCATTG ACGTCAATAG GGGCGTACTG GCATATGAAT ACACTGAATG TACTGGCTAG
         1021 TGGCAGTTAA CGTTAATACT CCAATCCATT GACGTCATGG AAAGTTCCTT ATTTGGCCGT
        1081 AC
        //
        Primer: mCherry-C_F
        Sequence file: RDB15451_A7Jvb.seq check
        >D05018B2_1_A7Jv_mCherry-C_F_C01_03_ABI08.ab1
            1 GACAAGAAAG CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC
           61 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC
          121 ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC GCTCGAGCCC
          181 AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA CCAGTGGCAG CCGCAAGCGC
          241 GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CAGGCCAGGC CACCGGCCCG CAGGAGACTG
          301 CGGCTGTCGG TGGACGAGGT TTCCAGCCCC AGTACCCCCG AGGCCCCAGA CATCCCAGCC
          361 TGCCCTTCTC CGGGCCAGAA GATAAAGAAA TCCACCCCGG CAGCAGGTCA GCCGCCCCAC
          421 CTGACATCCG CGCAGGACCA GGACACCATC ACCGGTCGCG CCAAGCGCGG CAGCGGCGCC
          481 ACCAACTTCA GCCTGCTGAA GCAGGCCGGC GACGTGGAGG AGAACCCCGG CCCCCGCGAA
          541 TTCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG
          601 GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC
          661 TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC
          721 ACCCTCGTGA CCACCCTGAG CTGGGGCGTG CAGTGCTTCG CCCGCTACCC CGACCACATG
          781 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGTACCATC
          841 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC
          901 CTGGTGAACC GCATCGAGCT GAATGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG
          961 GCACAAGCTG GAGTACAACT ACATCAGCGA CAACGTCTAT ATCACCGCCG ACAAGCAGAA
         1021 GAACGGCATC AAGGCCAACT TCAAGATCCG CCACACCATC GAGACCGCGG GCGTGCCAGC
         1081 TCGGCGAACA ACTAACAGGC AGACATCGCC CATTCGGTCG AACTGCTCGA TGCTGCTTGA
        1141 CCTGGTAGAC
        //
        Primer: PRE_rev
        Sequence file: RDB15451_A7Jvc.seq check
        >D05018B2_1_A7Jv_PRE_rev_D08_11_ABI24.ab1
            1 CGCTTCCCCA ATAGCGTAAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA
           61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
          121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT
          181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT
          241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC
          301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG
          361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT
          421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC
          481 CCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA
          541 CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGCTT GGACTGGGTG CTCAGGTAGT
          601 GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG
          661 CGAGCTGCAC GCCGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT
          721 TCTTCTGCTT GTCGGCGGTG ATATAGACGT TGTCGCTGAT GTAGTTGTAC TCCAGCTTGT
          781 GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA
          841 GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA
          901 AGATGGTACG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT
          961 TCATGTGGTC GGGGTAGCGG GCGAAGCACT GCACGCCCCA GCTCAGGTTG GTCACGAGGG
         1021 TGGGCCCAGG GCACGGCAGC TTGCCGTGCT GCAGATGGAC CTTCCAGGAT CAGCTTGGCC
         1081 CGTAAGTGCA ATCGATCCGT CGTCCTCGCT GGACACGCCT GGATCTGTAC CGATTACGAT
        1141 CGATG
        //
        Primer: HIVgp_R1
        Sequence file: RDB15451_A7Jvd.seq check
        >D5018B2_1_A7Jv_HIVgp_R1_B01_02_ABI08.ab1
            1 CGGAATCGTA TTCCACTGCT CTTTTTTCTC TCTGCACCAC TCTTCTCTTT GCCTTGGTGG
           61 GTGCTACTCC TAATGGTTCA ATTTTTACTA CTTTATATTT ATATAATTCA CTTCTCCAAT
          121 TGTCCCTCAT ATCTCCTCCT CCAGGTCTGA AGATCAGCGG CCGGCCGCTT GCTGTGCGGT
          181 GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG TGTCTTTTAT
          241 CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG ATCTAAGTTC
          301 TTCTGATCCT GTCTGAAGGG ATGGTTGTAG CTGTCCCAGT ATTTGTCTAC AGCCTTCTGA
          361 TGTTTCTAAC AGGCCAGGAT TAACTGCGAA TCGTTCTAGC TCCCTGCTTG CCCATACTAT
          421 ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CGAATTTTTT CCCATCGCGA
          481 TCTAATTCTC CCCCGCTTAA TACTGACGCT CTCGCACCCA TCTCTCTCCT TCTAGCCTCC
          541 GCTAGTCAAA ATTTTTGGCG TACTCACCAG TCGCCGCCCC TCGCCTCTTG CCGTGCGCGC
          601 TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGT TTCCCTTTCG CTTTCAAGTC
          661 CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC
          721 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT
          781 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG
          841 TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT CCCACCGTAC
          901 ACGCCTACCG CCCATTTGCG TCAATGGGGG CGGAGTTGTT ACGACATTTT TGGAAAGTCC
          961 CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGGTGGA GACTTGGAAA
         1021 TCCCCGTGAG TCAAACCCGC TATCCACGCC CATTGATGTA CTGCCCAAAA CGCATCACAT
         1081 GGTAATAGCG ATGACTATAC GTAGATGTAC CTGGCCCAAG CTAGGAAGGT TCCCCAATAA
        1141 GGTTCAATGG
        //
        Primer: BGH_rev2
        Sequence file: RDB15451_A7Jve.seq check
        >D5018B2_1_A7Jv_BGH_rev2_C01_03_ABI08.ab1
            1 CATTGAAGGG GTAGGAGGCA CGGGGGAGGG GCAACAACAG ATGGCTGGCA ACTAGAAGGC
           61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
          121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
          181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
          241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG
          301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG
          361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC
          421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG
          481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG
          541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
          601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
          661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
          721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
          781 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG
          841 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG
          901 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA
          961 GGCGAGCAGC CATGGAAAGG GACGTCAGCT TCCCCGACAA CACCACGGGA ATTGTCAGTG
         1021 CCCACAGCCC GAGCCCCTGT CAGCAGCGGC AGGCAGGCGG CGAATGAGTC GCGGTGTCAA
        1081 TAGGAAGGGG AAGCGAAGTC CCGGAAAGGA GCTGACAGGA TGAGTTGCCA
        //
        Primer: SV40pro_F
        Sequence file: RDB15451_A7Jvf.seq check
        >D05018B2_1_A7Jv_SVpro40_F_B01_02_ABI08.ab1
            1 CCCTGGTCCC GTGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
           61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
          121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
          181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT
          241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT
          301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC
          361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT
          421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG
          481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA
          541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
          601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
          661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
          721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
          781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC
          841 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
          901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
          961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
         1021 TTAATGAAAT CGGCCAACGC GCGGGGAGGA GGCGGTTTTG CGTATGGGCG CTCTTCGGCA
         1081 TCTTCGCTCA CTGACTCGCT GCGCTCGGAT CGGTCGCTGC GCGAGTGGTA ATCTCCAGAG
        1141 C
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        2022.07.13

        GNP_filter3_RDBDEP_html_220713.pl