RDB15451 - tFucci(SA)2.2/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内)

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提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. 付加的提供条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

References and tips

QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15451	tFucci(SA)2.2/pCSII-CMV	DNA solution

original	Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

Featured content	Fucci-related materials (en) (English text)
Featured content	Fucci-related materials (ja) (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: mCherry-C_R Sequence file: RDB15451_A7Jva.seq
>D05018B2_1_A7Jv_mCherry-C_R_B02_05_ABI24.ab1 1 GGTGGGAGTA GTCACTTGAT GTTGACGTTG TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG 61 GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC 121 TGCTTGATCT CGCCCTTCAG GGCGCCGTCC TCGGGGTACA TCCGCTCGGA GGAGGCCTCC 181 CAGCCCATGG TCTTCTTCTG CATTACGGGG CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC 241 TTCACCTTGT AGATGAACTC GCCGTCCTGC AGGGAGGAGT CCTGGGTCAC GGTCACCACG 301 CCGCCGTCCT CGAAGTTCAT CACGCGCTCC CACTTGAAGC CCTCGGGGAA GGACAGCTTC 361 AAGTAGTCGG GGATGTCGGC GGGGTGCTTC ACGTAGGCCT TGGAGCCGTA CATGAACTGA 421 GGGGACAGGA TGTCCCAGGC GAAGGGCAGG GGGCCACCCT TGGTCACCTT CAGCTTGGCG 481 GTCTGGGTGC CCTCGTAGGG GCGGCCCTCG CCCTCGCCCT CGATCTCGAA CTCGTGGCCG 541 TTCACGGAGC CCTCCATGTG CACCTTGAAG CGCATGAACT CCTTGATGAT GGCCATGTTA 601 TCCTCCTCGC CCTTGCTCAC CATGGTGGAT CCTATGCTAG CGGATCTGAC GGTTCACTAA 661 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG 721 GGCGGAGTTG TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC 781 ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC 841 ATTGATGTAC TGCCAAAACC GCATCACCAT GGGTAATAGC GATGACTAAT ACGTAGATGT 901 ACTGCCCAAG TAGGAAAGTC CCATACTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT 961 ACCGTCATTG ACGTCAATAG GGGCGTACTG GCATATGAAT ACACTGAATG TACTGGCTAG 1021 TGGCAGTTAA CGTTAATACT CCAATCCATT GACGTCATGG AAAGTTCCTT ATTTGGCCGT 1081 AC //
Primer: mCherry-C_F Sequence file: RDB15451_A7Jvb.seq
>D05018B2_1_A7Jv_mCherry-C_F_C01_03_ABI08.ab1 1 GACAAGAAAG CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC 61 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC 121 ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC GCTCGAGCCC 181 AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA CCAGTGGCAG CCGCAAGCGC 241 GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CAGGCCAGGC CACCGGCCCG CAGGAGACTG 301 CGGCTGTCGG TGGACGAGGT TTCCAGCCCC AGTACCCCCG AGGCCCCAGA CATCCCAGCC 361 TGCCCTTCTC CGGGCCAGAA GATAAAGAAA TCCACCCCGG CAGCAGGTCA GCCGCCCCAC 421 CTGACATCCG CGCAGGACCA GGACACCATC ACCGGTCGCG CCAAGCGCGG CAGCGGCGCC 481 ACCAACTTCA GCCTGCTGAA GCAGGCCGGC GACGTGGAGG AGAACCCCGG CCCCCGCGAA 541 TTCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG 601 GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC 661 TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC 721 ACCCTCGTGA CCACCCTGAG CTGGGGCGTG CAGTGCTTCG CCCGCTACCC CGACCACATG 781 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGTACCATC 841 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC 901 CTGGTGAACC GCATCGAGCT GAATGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG 961 GCACAAGCTG GAGTACAACT ACATCAGCGA CAACGTCTAT ATCACCGCCG ACAAGCAGAA 1021 GAACGGCATC AAGGCCAACT TCAAGATCCG CCACACCATC GAGACCGCGG GCGTGCCAGC 1081 TCGGCGAACA ACTAACAGGC AGACATCGCC CATTCGGTCG AACTGCTCGA TGCTGCTTGA 1141 CCTGGTAGAC //
Primer: PRE_rev Sequence file: RDB15451_A7Jvc.seq
>D05018B2_1_A7Jv_PRE_rev_D08_11_ABI24.ab1 1 CGCTTCCCCA ATAGCGTAAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA 61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT 121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT 181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT 241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC 301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG 361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT 421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC 481 CCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA 541 CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGCTT GGACTGGGTG CTCAGGTAGT 601 GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG 661 CGAGCTGCAC GCCGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT 721 TCTTCTGCTT GTCGGCGGTG ATATAGACGT TGTCGCTGAT GTAGTTGTAC TCCAGCTTGT 781 GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA 841 GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA 901 AGATGGTACG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT 961 TCATGTGGTC GGGGTAGCGG GCGAAGCACT GCACGCCCCA GCTCAGGTTG GTCACGAGGG 1021 TGGGCCCAGG GCACGGCAGC TTGCCGTGCT GCAGATGGAC CTTCCAGGAT CAGCTTGGCC 1081 CGTAAGTGCA ATCGATCCGT CGTCCTCGCT GGACACGCCT GGATCTGTAC CGATTACGAT 1141 CGATG //
Primer: HIVgp_R1 Sequence file: RDB15451_A7Jvd.seq
>D5018B2_1_A7Jv_HIVgp_R1_B01_02_ABI08.ab1 1 CGGAATCGTA TTCCACTGCT CTTTTTTCTC TCTGCACCAC TCTTCTCTTT GCCTTGGTGG 61 GTGCTACTCC TAATGGTTCA ATTTTTACTA CTTTATATTT ATATAATTCA CTTCTCCAAT 121 TGTCCCTCAT ATCTCCTCCT CCAGGTCTGA AGATCAGCGG CCGGCCGCTT GCTGTGCGGT 181 GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG TGTCTTTTAT 241 CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG ATCTAAGTTC 301 TTCTGATCCT GTCTGAAGGG ATGGTTGTAG CTGTCCCAGT ATTTGTCTAC AGCCTTCTGA 361 TGTTTCTAAC AGGCCAGGAT TAACTGCGAA TCGTTCTAGC TCCCTGCTTG CCCATACTAT 421 ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CGAATTTTTT CCCATCGCGA 481 TCTAATTCTC CCCCGCTTAA TACTGACGCT CTCGCACCCA TCTCTCTCCT TCTAGCCTCC 541 GCTAGTCAAA ATTTTTGGCG TACTCACCAG TCGCCGCCCC TCGCCTCTTG CCGTGCGCGC 601 TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGT TTCCCTTTCG CTTTCAAGTC 661 CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC 721 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT 781 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG 841 TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT CCCACCGTAC 901 ACGCCTACCG CCCATTTGCG TCAATGGGGG CGGAGTTGTT ACGACATTTT TGGAAAGTCC 961 CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGGTGGA GACTTGGAAA 1021 TCCCCGTGAG TCAAACCCGC TATCCACGCC CATTGATGTA CTGCCCAAAA CGCATCACAT 1081 GGTAATAGCG ATGACTATAC GTAGATGTAC CTGGCCCAAG CTAGGAAGGT TCCCCAATAA 1141 GGTTCAATGG //
Primer: BGH_rev2 Sequence file: RDB15451_A7Jve.seq
>D5018B2_1_A7Jv_BGH_rev2_C01_03_ABI08.ab1 1 CATTGAAGGG GTAGGAGGCA CGGGGGAGGG GCAACAACAG ATGGCTGGCA ACTAGAAGGC 61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA 121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG 181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG 241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG 301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG 361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC 421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG 481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG 541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC 601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA 661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC 721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC 781 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG 841 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG 901 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA 961 GGCGAGCAGC CATGGAAAGG GACGTCAGCT TCCCCGACAA CACCACGGGA ATTGTCAGTG 1021 CCCACAGCCC GAGCCCCTGT CAGCAGCGGC AGGCAGGCGG CGAATGAGTC GCGGTGTCAA 1081 TAGGAAGGGG AAGCGAAGTC CCGGAAAGGA GCTGACAGGA TGAGTTGCCA //
Primer: SV40pro_F Sequence file: RDB15451_A7Jvf.seq
>D05018B2_1_A7Jv_SVpro40_F_B01_02_ABI08.ab1 1 CCCTGGTCCC GTGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA 61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA 121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC 181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT 241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT 301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC 361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT 421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG 481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA 541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC 841 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC 961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA 1021 TTAATGAAAT CGGCCAACGC GCGGGGAGGA GGCGGTTTTG CGTATGGGCG CTCTTCGGCA 1081 TCTTCGCTCA CTGACTCGCT GCGCTCGGAT CGGTCGCTGC GCGAGTGGTA ATCTCCAGAG 1141 C //