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Inverse pericam/pcDNA3

Calcium ion sensor clone Inverse pericam having a circularly permuted YFP and a CaM.

Catalog number RDB15140
Resource name Inverse pericam/pcDNA3
Clone info. Calcium ion sensor clone Inverse pericam having a circularly permuted YFP and a CaM. Becoming dimmed with Ca2+. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank
External Database
X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP
        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form         [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件は英文のまま記載してください。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Availability Shipping form Fee (non-profit org.)
        RDB15140 Inverse pericam/pcDNA3 Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References and tips

        Original reference

        original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Calcium Ion Sensor Clones (English text)

        QC test results

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References

        Original, user report and related articles

        original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

        2023.04.10

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