RDB15140 - Inverse pericam/pcDNA3

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Inverse pericam/pcDNA3 (#RDB15140)

Calcium ion sensor clone Inverse pericam having a circularly permuted YFP and a CaM.

Clone info.	Calcium ion sensor clone Inverse pericam having a circularly permuted YFP and a CaM. Becoming dimmed with Ca2+. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition.
Vector backbone	pcDNA3 (plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name	X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001).

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001).
備考	提供条件は英文のまま記載してください。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB15140	Inverse pericam/pcDNA3	Under QC test. Please contact us.	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The Inverse pericam/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15140).

Reference section:

Nagai, T., Sawano, A., Park, E.S., Miyawaki, A., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. [link to RRC of NBRP]