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UnaG/pGEX-2T

Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.

Catalog number RDB15704
Resource name UnaG/pGEX-2T
Clone info. Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.
Note: This clone has lacI instead of lacIq promoter. It was revealed by our quality test.
Vector backbone pGEX-2T (Plasmid)
Selectable markers Amp^r
Gene/insert name Anguilla japonica (Japanese eel) UnaG cDNA
Reference of nucleotide sequence AB763906 (DDBJ accession number)
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Kumagai, A., Ando. R,, Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A. (2013) A Bilirubin-Inducible Fluorescent Protein from Eel Muscle. Cell, 153 (7): 1602-1611.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
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Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15704 UnaG/pGEX-2T DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)
Featured content Sensor and Visualization (English text)

References

original Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15704_A7Lcp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 RDB15704_A7Lca.seq
>D05018C8_A7Lc_2_pGEX-F2_F02_17_ABI24.ab1
    1 GCTAGTAGCA TGATTTAAAC GTATTGAAGC TATCCCACAA ATTGATAAGT ACTTGAAATC
   61 CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG GTGGCGACCA
  121 TCCTCCAAAA TCGGATCTGG TTCCGCGTGG ATCCGGTGGT TCTGGTATGG TCGAGAAATT
  181 TGTTGGCACC TGGAAGATCG CAGACAGCCA TAATTTTGGT GAATACCTGA AAGCTATCGG
  241 AGCCCCAAAG GAATTAAGCG ATGGTGGGGA TGCCACGACG CCGACATTGT ACATCTCCCA
  301 GAAGGACGGA GACAAAATGA CAGTGAAAAT AGAGAATGGA CCTCCTACGT TCCTTGACAC
  361 TCAAGTAAAG TTCAAATTAG GGGAGGAGTT CGACGAATTT CCTTCTGATC GAAGAAAAGG
  421 CGTAAAATCT GTCGTGAACT TGGTGGGAGA GAAGCTGGTG TACGTACAAA AGTGGGACGG
  481 CAAGGAGACG ACGTATGTCC GAGAGATAAA GGACGGTAAA CTGGTCGTGA CACTTACGAT
  541 GGGAGACGTC GTGGCTGTGC GCAGCTACCG GAGGGCGACG GAATGAGAAT TCATCGTGAC
  601 TGACTGACGA TCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG
  661 CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG
  721 GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT
  781 AGCGGAGTGT ATAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTTATAGGT
  841 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGG CACTTTTTCG GGGAAATGTG
  901 CGCGGAACCC CTATTTGTTT ATTTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG
  961 ACAATAACCC TGATAAATGC TTCAATAATA TTTGAAAAAG GAGAGTATGA GTATTCAACC
 1021 ATTTTCCGTG TCGCCCTATT CCCTTTTTTT GCGGCATTTT GACTTCCTTG TGTGCTCACC
1081 AGGAACGCTG GGTGTGCA
//
Primer: pGEX-ptac_up-F RDB15704_A7Lcb.seq
>D05018C8_A7Lc_2_pGEX-ptac_up-F_G02_20_ABI24.ab1
    1 GATACCGGCT CATAACGGTT CTGGCAATAT TCTGAATGAG CTGTTGACAA TTAATCATCG
   61 GCTCGTATAA TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGTATTCAT
  121 GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT
  181 GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG
  241 GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG
  301 TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT
  361 GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA
  421 TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA
  481 TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC
  541 ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT
  601 TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA
  661 ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG
  721 GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATCT
  781 GGTTCCGCGT GGATCCGGTG GTTCTGGTAT GGTCGAGAAA TTTGTTGGCA CCTGGAAGAT
  841 CGCAGACAGC CATAATTTTG GTGAATTCCT GAAAGCTATC GGAGCCCCCA AAAGGAATTA
  901 AGCGATGGGT GGGGATGCCA CGACGCCCGA CATTGTACAT CTCCAAGAAA GGACGGAGAC
  961 AAAATGACAG GTGAAATAGA GAATGGACCC TCCTACGTTC CTTTGACACT CAGGAAAGGT
 1021 TTCAAAATTA CGGGGGAGGG AGTTCGATCG AATTTTCCTT CTTGATCGTA GTAATATGGC
1081 TGTATAAATC TTGGTTCGAT GGTATTC
//
Primer: M13_-40 RDB15704_A7Lcc.seq
>D05018C8_A7Lc_2_M13_-40_H02_23_ABI24.ab1
    1 CGGGCGCTAT CGTATCATGG TCATAGCTGT TTCCTGTGTG AATTGTTATC CGCTCACAAT
   61 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG
  121 CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
  181 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA
  241 GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC
  301 CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT
  361 TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA
  421 CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG
  481 CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA
  541 TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA
  601 TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC
  661 TTAATGGGCC CGCTAAACAG CGCGATTTGC TGGTGACCCA AATGCGACCA GATGCTCCAC
  721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA
  781 GACATCAAGA AATAAACGGC CGGAACATTA GTGCAGGGCA AGCTTCCCAC AGCAATGGGC
  841 ATCCCTGGGT CATCCCAGCG GGATAAGTTT AATTGATCAA GCCCCACCTG ATCGCCGTTT
  901 GCGCGAAGAA AGATTGTCGC AACCCGCCCG CCTTTAACAG GGCTTCAACG CCCGGCTTTC
961 GTTTCCTACC CATCCGACAC CCACCACGGC TG
//
Primer: SV40pA3'ElementRverse RDB15704_A7Lcd.seq
>15704_15704_A9Da_SV40pA3'ElementRverse_D01_04_ABI08.ab1
    1 GTGCGTCGGG GGGGGCGGGA GCCTATGGAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
   61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
  121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
  181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG
  241 CATCTGTGCG GTATTTCACA CCGCATAAAT TCCGACACCA TCGAATGGCG CAAAACCTTT
  301 CGCGGTATGG CATGATAGCG CCCGGAAGAG AGTCAATTCA GGGTGGTGAA TGTGAAACCA
  361 GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG
  421 GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG
  481 GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG
  541 ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT
  601 AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC
  661 GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC
  721 ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGGCTGTG GAAGCTGCCC TGGCACTAAA
  781 TGTTCCGGCG TTATTTCTTT GATGTCTCTG ACCAGAACAC CCCATCAACA GTATTTATTT
  841 TTCTTCCCAT GAAGAACGGT ACCGCGACTT GGGGCGTGAA GCATTCTGGT GCGCATTGGG
901 GTCACCCGGC AAATGCGCGC CTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684.
reference Shitashima, Y., Two Distinct Fluorescence States of the Ligand-Induced Green Fluorescent Protein UnaG. Biophys. J. 113 (12): 2805-2814 (2017). PMID 29262373.
reference Iwatani, S., Fluorescent protein-based detection of unconjugated bilirubin in newborn serum. Sci. Rep. 6: 28489 (2016). PMID 27324682.
reference Miyawaki, A., Exploiting the cyanobacterial light-harvesting machinery for developing fluorescent probes. Nat. Methods 13 (9): 729-730 (2016). PMID 27575623.

2019.04.16

GNP_filter3_RDBDEP_html_190327.pl