RDB15704 - UnaG/pGEX-2T

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UnaG/pGEX-2T (#RDB15704)

Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.

Clone info.	Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.
Comment	Note: This clone has lacI instead of lacIq promoter. It was revealed by our quality test.
Vector backbone	pGEX-2T (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Anguilla japonica (Japanese eel) UnaG cDNA
Reference of insert sequence	AB763906 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

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Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).

Remarks ((Additional form)) Approval Form(FormD)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).
Remarks	((Additional form)) Approval Form(FormD)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).
備考	【追加提供依頼書】承諾書(FormD) 提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15704	UnaG/pGEX-2T	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The UnaG/pGEX-2T was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15704).

Reference section:

Kumagai, A., Ando, R., Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15704_A7Lcp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 () Sequence file: RDB15704_A7Lca.seq
>D05018C8_A7Lc_2_pGEX-F2_F02_17_ABI24.ab1 1 GCTAGTAGCA TGATTTAAAC GTATTGAAGC TATCCCACAA ATTGATAAGT ACTTGAAATC 61 CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG GTGGCGACCA 121 TCCTCCAAAA TCGGATCTGG TTCCGCGTGG ATCCGGTGGT TCTGGTATGG TCGAGAAATT 181 TGTTGGCACC TGGAAGATCG CAGACAGCCA TAATTTTGGT GAATACCTGA AAGCTATCGG 241 AGCCCCAAAG GAATTAAGCG ATGGTGGGGA TGCCACGACG CCGACATTGT ACATCTCCCA 301 GAAGGACGGA GACAAAATGA CAGTGAAAAT AGAGAATGGA CCTCCTACGT TCCTTGACAC 361 TCAAGTAAAG TTCAAATTAG GGGAGGAGTT CGACGAATTT CCTTCTGATC GAAGAAAAGG 421 CGTAAAATCT GTCGTGAACT TGGTGGGAGA GAAGCTGGTG TACGTACAAA AGTGGGACGG 481 CAAGGAGACG ACGTATGTCC GAGAGATAAA GGACGGTAAA CTGGTCGTGA CACTTACGAT 541 GGGAGACGTC GTGGCTGTGC GCAGCTACCG GAGGGCGACG GAATGAGAAT TCATCGTGAC 601 TGACTGACGA TCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG 661 CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG 721 GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT 781 AGCGGAGTGT ATAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTTATAGGT 841 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGG CACTTTTTCG GGGAAATGTG 901 CGCGGAACCC CTATTTGTTT ATTTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG 961 ACAATAACCC TGATAAATGC TTCAATAATA TTTGAAAAAG GAGAGTATGA GTATTCAACC 1021 ATTTTCCGTG TCGCCCTATT CCCTTTTTTT GCGGCATTTT GACTTCCTTG TGTGCTCACC 1081 AGGAACGCTG GGTGTGCA //
Primer: pGEX-ptac_up-F () Sequence file: RDB15704_A7Lcb.seq
>D05018C8_A7Lc_2_pGEX-ptac_up-F_G02_20_ABI24.ab1 1 GATACCGGCT CATAACGGTT CTGGCAATAT TCTGAATGAG CTGTTGACAA TTAATCATCG 61 GCTCGTATAA TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGTATTCAT 121 GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT 181 GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG 241 GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG 301 TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT 361 GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA 421 TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA 481 TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC 541 ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT 601 TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA 661 ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG 721 GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATCT 781 GGTTCCGCGT GGATCCGGTG GTTCTGGTAT GGTCGAGAAA TTTGTTGGCA CCTGGAAGAT 841 CGCAGACAGC CATAATTTTG GTGAATTCCT GAAAGCTATC GGAGCCCCCA AAAGGAATTA 901 AGCGATGGGT GGGGATGCCA CGACGCCCGA CATTGTACAT CTCCAAGAAA GGACGGAGAC 961 AAAATGACAG GTGAAATAGA GAATGGACCC TCCTACGTTC CTTTGACACT CAGGAAAGGT 1021 TTCAAAATTA CGGGGGAGGG AGTTCGATCG AATTTTCCTT CTTGATCGTA GTAATATGGC 1081 TGTATAAATC TTGGTTCGAT GGTATTC //
Primer: M13_-40 () Sequence file: RDB15704_A7Lcc.seq
>D05018C8_A7Lc_2_M13_-40_H02_23_ABI24.ab1 1 CGGGCGCTAT CGTATCATGG TCATAGCTGT TTCCTGTGTG AATTGTTATC CGCTCACAAT 61 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG 121 CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 181 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA 241 GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC 301 CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT 361 TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA 421 CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG 481 CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA 541 TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA 601 TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC 661 TTAATGGGCC CGCTAAACAG CGCGATTTGC TGGTGACCCA AATGCGACCA GATGCTCCAC 721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA 781 GACATCAAGA AATAAACGGC CGGAACATTA GTGCAGGGCA AGCTTCCCAC AGCAATGGGC 841 ATCCCTGGGT CATCCCAGCG GGATAAGTTT AATTGATCAA GCCCCACCTG ATCGCCGTTT 901 GCGCGAAGAA AGATTGTCGC AACCCGCCCG CCTTTAACAG GGCTTCAACG CCCGGCTTTC 961 GTTTCCTACC CATCCGACAC CCACCACGGC TG //
Primer: SV40pA3'ElementRverse () Sequence file: RDB15704_A7Lcd.seq
>15704_15704_A9Da_SV40pA3'ElementRverse_D01_04_ABI08.ab1 1 GTGCGTCGGG GGGGGCGGGA GCCTATGGAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG 241 CATCTGTGCG GTATTTCACA CCGCATAAAT TCCGACACCA TCGAATGGCG CAAAACCTTT 301 CGCGGTATGG CATGATAGCG CCCGGAAGAG AGTCAATTCA GGGTGGTGAA TGTGAAACCA 361 GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG 421 GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG 481 GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG 541 ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT 601 AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC 661 GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC 721 ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGGCTGTG GAAGCTGCCC TGGCACTAAA 781 TGTTCCGGCG TTATTTCTTT GATGTCTCTG ACCAGAACAC CCCATCAACA GTATTTATTT 841 TTCTTCCCAT GAAGAACGGT ACCGCGACTT GGGGCGTGAA GCATTCTGGT GCGCATTGGG 901 GTCACCCGGC AAATGCGCGC CTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684. [link to RRC of NBRP]
reference	Shitashima, Y., Two Distinct Fluorescence States of the Ligand-Induced Green Fluorescent Protein UnaG. Biophys. J. 113 (12): 2805-2814 (2017). PMID 29262373.
reference	Iwatani, S., Fluorescent protein-based detection of unconjugated bilirubin in newborn serum. Sci. Rep. 6: 28489 (2016). PMID 27324682.
review	Miyawaki, A., Exploiting the cyanobacterial light-harvesting machinery for developing fluorescent probes. Nat. Methods 13 (9): 729-730 (2016). PMID 27575623.

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External Database
Anguilla japonica (Japanese eel) UnaG

Reference sequence