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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

UnaG/pGEX-2T

Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.

Catalog number RDB15704
Resource name UnaG/pGEX-2T
Clone info. Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.
Comment Note: This clone has lacI instead of lacIq promoter. It was revealed by our quality test.
Vector backbone pGEX-2T (Plasmid)
Selectable markers Amp^r
Gene/insert name Anguilla japonica (Japanese eel) UnaG cDNA
Reference of insert sequence AB763906 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Anguilla japonica (Japanese eel) UnaG

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Kumagai, A., Ando. R,, Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A. (2013) A Bilirubin-Inducible Fluorescent Protein from Eel Muscle. Cell, 153 (7): 1602-1611.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks ((Additional form)) Approval Form(FormD)
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Kumagai, A., Ando. R,, Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A. (2013) A Bilirubin-Inducible Fluorescent Protein from Eel Muscle. Cell, 153 (7): 1602-1611.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 【追加提供依頼書】承諾書(FormD)
            提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15704 UnaG/pGEX-2T DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Miyawaki, A., Novel polypeptide exhibiting fluorescent properties and use thereof. Patent publication US 2016 0009771 A1.
            original Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content Sensor and Visualization (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15704_A7Lcp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pGEX-F2
            Sequence file: RDB15704_A7Lca.seq check
            >D05018C8_A7Lc_2_pGEX-F2_F02_17_ABI24.ab1
                1 GCTAGTAGCA TGATTTAAAC GTATTGAAGC TATCCCACAA ATTGATAAGT ACTTGAAATC
               61 CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG GTGGCGACCA
              121 TCCTCCAAAA TCGGATCTGG TTCCGCGTGG ATCCGGTGGT TCTGGTATGG TCGAGAAATT
              181 TGTTGGCACC TGGAAGATCG CAGACAGCCA TAATTTTGGT GAATACCTGA AAGCTATCGG
              241 AGCCCCAAAG GAATTAAGCG ATGGTGGGGA TGCCACGACG CCGACATTGT ACATCTCCCA
              301 GAAGGACGGA GACAAAATGA CAGTGAAAAT AGAGAATGGA CCTCCTACGT TCCTTGACAC
              361 TCAAGTAAAG TTCAAATTAG GGGAGGAGTT CGACGAATTT CCTTCTGATC GAAGAAAAGG
              421 CGTAAAATCT GTCGTGAACT TGGTGGGAGA GAAGCTGGTG TACGTACAAA AGTGGGACGG
              481 CAAGGAGACG ACGTATGTCC GAGAGATAAA GGACGGTAAA CTGGTCGTGA CACTTACGAT
              541 GGGAGACGTC GTGGCTGTGC GCAGCTACCG GAGGGCGACG GAATGAGAAT TCATCGTGAC
              601 TGACTGACGA TCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG
              661 CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG
              721 GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT
              781 AGCGGAGTGT ATAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTTATAGGT
              841 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGG CACTTTTTCG GGGAAATGTG
              901 CGCGGAACCC CTATTTGTTT ATTTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG
              961 ACAATAACCC TGATAAATGC TTCAATAATA TTTGAAAAAG GAGAGTATGA GTATTCAACC
             1021 ATTTTCCGTG TCGCCCTATT CCCTTTTTTT GCGGCATTTT GACTTCCTTG TGTGCTCACC
            1081 AGGAACGCTG GGTGTGCA
            //
            Primer: pGEX-ptac_up-F
            Sequence file: RDB15704_A7Lcb.seq check
            >D05018C8_A7Lc_2_pGEX-ptac_up-F_G02_20_ABI24.ab1
                1 GATACCGGCT CATAACGGTT CTGGCAATAT TCTGAATGAG CTGTTGACAA TTAATCATCG
               61 GCTCGTATAA TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGTATTCAT
              121 GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT
              181 GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG
              241 GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG
              301 TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT
              361 GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA
              421 TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA
              481 TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC
              541 ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT
              601 TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA
              661 ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG
              721 GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATCT
              781 GGTTCCGCGT GGATCCGGTG GTTCTGGTAT GGTCGAGAAA TTTGTTGGCA CCTGGAAGAT
              841 CGCAGACAGC CATAATTTTG GTGAATTCCT GAAAGCTATC GGAGCCCCCA AAAGGAATTA
              901 AGCGATGGGT GGGGATGCCA CGACGCCCGA CATTGTACAT CTCCAAGAAA GGACGGAGAC
              961 AAAATGACAG GTGAAATAGA GAATGGACCC TCCTACGTTC CTTTGACACT CAGGAAAGGT
             1021 TTCAAAATTA CGGGGGAGGG AGTTCGATCG AATTTTCCTT CTTGATCGTA GTAATATGGC
            1081 TGTATAAATC TTGGTTCGAT GGTATTC
            //
            Primer: M13_-40
            Sequence file: RDB15704_A7Lcc.seq check
            >D05018C8_A7Lc_2_M13_-40_H02_23_ABI24.ab1
                1 CGGGCGCTAT CGTATCATGG TCATAGCTGT TTCCTGTGTG AATTGTTATC CGCTCACAAT
               61 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG
              121 CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
              181 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA
              241 GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC
              301 CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT
              361 TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA
              421 CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG
              481 CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA
              541 TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA
              601 TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC
              661 TTAATGGGCC CGCTAAACAG CGCGATTTGC TGGTGACCCA AATGCGACCA GATGCTCCAC
              721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA
              781 GACATCAAGA AATAAACGGC CGGAACATTA GTGCAGGGCA AGCTTCCCAC AGCAATGGGC
              841 ATCCCTGGGT CATCCCAGCG GGATAAGTTT AATTGATCAA GCCCCACCTG ATCGCCGTTT
              901 GCGCGAAGAA AGATTGTCGC AACCCGCCCG CCTTTAACAG GGCTTCAACG CCCGGCTTTC
            961 GTTTCCTACC CATCCGACAC CCACCACGGC TG
            //
            Primer: SV40pA3'ElementRverse
            Sequence file: RDB15704_A7Lcd.seq check
            >15704_15704_A9Da_SV40pA3'ElementRverse_D01_04_ABI08.ab1
                1 GTGCGTCGGG GGGGGCGGGA GCCTATGGAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
               61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
              121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
              181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG
              241 CATCTGTGCG GTATTTCACA CCGCATAAAT TCCGACACCA TCGAATGGCG CAAAACCTTT
              301 CGCGGTATGG CATGATAGCG CCCGGAAGAG AGTCAATTCA GGGTGGTGAA TGTGAAACCA
              361 GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG
              421 GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG
              481 GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG
              541 ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT
              601 AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC
              661 GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC
              721 ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGGCTGTG GAAGCTGCCC TGGCACTAAA
              781 TGTTCCGGCG TTATTTCTTT GATGTCTCTG ACCAGAACAC CCCATCAACA GTATTTATTT
              841 TTCTTCCCAT GAAGAACGGT ACCGCGACTT GGGGCGTGAA GCATTCTGGT GCGCATTGGG
            901 GTCACCCGGC AAATGCGCGC CTG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Miyawaki, A., Novel polypeptide exhibiting fluorescent properties and use thereof. Patent publication US 2016 0009771 A1.
            original Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684.
            reference Shitashima, Y., Two Distinct Fluorescence States of the Ligand-Induced Green Fluorescent Protein UnaG. Biophys. J. 113 (12): 2805-2814 (2017). PMID 29262373.
            reference Iwatani, S., Fluorescent protein-based detection of unconjugated bilirubin in newborn serum. Sci. Rep. 6: 28489 (2016). PMID 27324682.
            review Miyawaki, A., Exploiting the cyanobacterial light-harvesting machinery for developing fluorescent probes. Nat. Methods 13 (9): 729-730 (2016). PMID 27575623.

            2022.05.17

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