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pBS coupler 4

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Catalog number RDB15129
Resource name pBS coupler 4
Clone info. Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
Vector backbone Plasmid
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15129 pBS coupler 4 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15129_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: insert, f1 ori | Primer: M13R | Sequence: RDB15129_A7H9a.seq
>D04498B5_A7H9_1_M13R_B04_04_ABI24.ab1
    1 TGCGCGCCAA TTACCCCCTT CCTAAGGGGG AACAAAGCTG GGGTACCGGG GCCCCCCCTC
   61 GAGGTCGACG GTATCGATAA GCTTGATCGA TCCGCCACCG CCAGAGCCAC CTCCGCCTGA
  121 ACCGCCTCCA CCATCGAATT CCTGCAGCCC GGGGGATCCA CTAGTTCTAG AGCGGCCGCC
  181 ACCGCGGTGG AGCTCCAATT CGCCCTATAG TGAGTCGTAT TACGCGCGCT CACTGGCCGT
  241 CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC
  301 ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA
  361 ACAGTTGCGC AGCCTGAATG GCGAATGGAA ATTGTAAGCG TTAATATTTT GTTAAAATTC
  421 GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC
  481 CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG
  541 AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC
  601 GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA
  661 GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAAGCCGGC
  721 GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGGCGCTAGG GCGCTGGCAA
  781 AGTGTAGCGG GTCACGCTGC GCGTAACCCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC
  841 AGGGGCGCGT CAGGTGGCAC TTTTCGGGGG AAATGTGCGC GGAACCGCCT AATTTGTTTA
  901 TTTTTTCTAA ATACAATTCA AATATGTATC CGCTCATGAG ACAATAAACC CCTGGATAAA
  961 TGCTTCAAAT AATATTGAAA AAAGGGAAGA GTATGAATTA TTTCAACATT TCCCGTGTTC
 1021 GCCCTTTATT CCCCTTTTTT TGGCGGCATT TTTGCCCTTT CCTTGTTTTT GCTTCACCCC
 1081 AAAAACGCTG GTGAAAGTAA AGGATGCTTG AAGATCAGTT GGGTGCCACC GAGTTGGGGT
 1141 TTACATCGAA CCTGGAATCT CTCAACCAGC GGGTTAAAAA TTCCTCTTGG AGAAGTGTTC
1201 CGGCCCTCGC AGAGCATAAC GCGTATTTTC CGCTCGCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471.

2019.11.02

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