RDB15444 - tFucci(SA)2.1/pCSII-EF

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tFucci(SA)2.1/pCSII-EF (#RDB15444)

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.

Clone info.	Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.
Vector backbone	CSII-EF-MCS (RDB04378) (plasmid)
Size of vector backbone	9.1 kb
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name	Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Anemonia majano CFP cDNA
Reference of insert sequence	LC192887 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
In order to receive this biological resource, please request the Exclusive MTA to us.
Please visit Ordering instruction.[link]

Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.

Remarks Remember that you will be working with samples containing infectious virus.
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Ordering instruction.[link]
Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. MTAに書く付加的使用条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15444	tFucci(SA)2.1/pCSII-EF	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The tFucci(SA)2.1/pCSII-EF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15444).

Reference section:

Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15444_B1K5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF1alpha pro,insert 5'(mCherry 5') Sequence file: RDB15444_B1K5a.seq
>15444_15444_B1K5_3_EF1a-C_F_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNTTTTGAN TTTGGNNCTT GGTTNNTTCT CAAGCCTCAG ACAGTGGTTC 61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAACACGCTA CCGGTCTCGA GAATTCTGCA 121 GCGGCCGCGG ATCCACCATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG 181 AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG 241 AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA 301 AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA 361 AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG 421 GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG 481 ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC 541 CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGANGCCTCC TCCGAGCGGA 601 TGTACCCCGA GGACGGCGCC CTGAANGGCG AGNTCAAGCA GANGCTGAAG CTGNNGACGG 661 CGGCCACTAC GACGCTGANG NCNAGANNNC CTACNNNNCC NAGANNCCNN TGNAGCTGCC 721 NNGNGCCNNN NNNN //
Primer: PRE_rev (Pr0250) Region: PRE,insert 3'(hGem(1_110), AmCyan1 3') Sequence file: RDB15444_B1K5b.seq
>15444_15444_B1K5_3_PRE_rev_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GNNCANCATA GTTAAGAATA CCAGTCAATC TTTCACAAAT 61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGATTAC 121 AGCGCCTTTC TCCGTTTTTC TGCCACTTCC TTCCAATACT GAGAGGATGG ATTTTCTTTA 181 ATCATAAGAT CAAATGACTC CTGGGTGACT CCTCCAAGAT TTTTATTTTC ACTAGATTCT 241 GGGACAATAA CCCCAGGGCT GGAAGTTGTA GATGTTAAGT GGTCATTCCG ATGTTTCCTT 301 TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA TGCAGAAGGC 361 TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC TTTGATTTCT 421 TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA TGGATATCCG 481 AAGGGCACCA CGGAGGTGAT GTGGGCCACG GCGTGCTCGG TCAGCTGCAC GCTGTTGCCG 541 CCCTTGTCCA GGTCGGTTCT GGCGATGCGG TGCTCCACCA CGTGGTTGGG GGGCATGGTC 601 ACGGGCTTCT TGGTCTTGTA NGANGTGTGG AACTGGCATC TGTAGTTGCC GCCGCCCTGC 661 AGCATCANGA ANGNGGNCNC GTCGCCCNTC NNNANGNCGT CNCANNCNGT CATCNNCTCN 721 ANNNNNGGGN NCNNNNNN //
Primer: mCherry-C_F (Pr0453) Region: insert mid(mCherry 3', hCdt1(30_120), P2A, AmCyan1 5') Sequence file: RDB15444_B1K5c.seq
>15444_15444_B1K5_3_mCherry-C_F_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN CCNNCCCCNG NCAGGCCCGC ANTCCGCGCC CCGGCCTCCG 61 CTAACCAGTG GCAGCCGCAA GCGCGCCCGC CCGCCCGCCG CCCCCGGACG CGACCAGGCC 121 AGGCCACCGG CCCGCAGGAG ACTGCGGCTG TCNGTGGACN AGGTTTCCAG CCCCAGTACC 181 CCCGAGGCCC CAGACATCCC AGCCTGCCCT TCTCCGGGCC AGAAGATAAA GAAATCCACC 241 CCGGCAGCAG GTCAGCCGCC CCACCTGACA TCCGCGCAGG ACCAGGACAC CATCACCGGT 301 CGCGCCAAGC GCGGCAGCGG CGCCACCAAC TTCAGCCTGC TGAAGCAGGC CGGCGACGTG 361 GAGGAGAACC CCGGCCCCCG CGAATTCGCC ACCATGGCCC TGTCCAACNN NTTCATCGGC 421 GACGACATGA AGATGACCTA CCACATGNNN NGCTGCGTGA ACGGCCACTA CTTCACCGTG 481 AAGGGCGAGG GCAGCGGCAA GCCCTACGAG GGCACCCANA CCTCCACCTT CAAGGTGACC 541 ATGGCCAACG GCGGCCCCCT GGCCTTCTCC TTCGACATCC TGTCCACCGT GTTCATGTAC 601 NGCAACCGCT GCTTCACCGN CTACNCCNNN CAGCATGNCC GACNACTTCA AGCANGCCTT 661 CCCCGACNGN ATGTCCTACG AGNNANNCNT TNACCTACNA GGANNGNNGG CGNNNNCANN 721 GNCAGNNNGN NNATCAGCCT GNNNNNNNNN GCNNNNNGCA NNNGTCNCCT TCNNNNNCGN 781 GNNCTTCCCN NNCGANGNNN NNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5'LTR,HIV-1 psi Sequence file: RDB15444_B1K5d.seq
>15444_15444_B1K5_3_CMV-Forward_D01_10_ABI24.ab1 1 NNNNNNNNNN NNNNNTTGNN NCNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC 121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA 181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG 241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC 301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG 361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG 421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT 481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC 541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC 601 TGGGACAGCT ACNACCNTCC CTTCNGACAG GNTCAGAAGA ACTTAGATCA TTATATAATA 661 CAGTAGCNAC CCTCNANNGT GTGCATCAAN NNANAGAGAN AAANNNNNNN NANGNANNCN 721 TNNNNNNNNN N //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi Sequence file: RDB15444_B1K5e.seq
>15444_15444_B1K5_3_HIVgp_R1_E01_13_ABI24.ab1 1 NNNNNNNNNN NNNNNCTCTT TTTTCTCTCT GCACCACTCT TCTCTTTGCC TTGGTGGGTG 61 CTACTCCTAA TGGTTCAATT TTTACTACTT TATATTTATA TAATTCACTT CTCCAATTGT 121 CCCTCATATC TCCTCCTCCA GGTCTGAAGA TCAGCGGCCC TTGCTGTGCG GTGGTCTTAC 181 TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT ATCTCTATCC 241 TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA TGATCTAAGT TCTTCTGATC 301 CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT ACAGCCTTCT GATGTTTCTA 361 ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT TGCCCATACT ATATGTTTTA 421 ATTTATATTT TTTCTTTCCC CCTGGCCTTA ACCGAATTTT TTCCCATCGC GATCTAATTC 481 TCCCCCGCTT AATACTGACG CTCTCGCACC CATCTCTCTC CTTCTAGCCT CCGCTAGTCA 541 AAATTTTTGG CGTACTCACC AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC GCTTCAGCAA 601 GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT CGCTTTCNAG TCCCTGTTCG 661 GGCGCCACTG CTAGAGANTT TCCNCACTGN CNNAANNNNN CTNANGGNNC TCTNGTNNNN 721 NGANTNNNNN NNNNN //
Primer: BGH_rev3 (Pr0885) Region: bGH pA,3'LTR Sequence file: RDB15444_B1K5f.seq
>15444_15444_B1K5_3_bGH_rev3_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNNGGNNGN NGGGCANACA ACAGATGGCT GGCAACTAGA AGGCACAGTC 61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG 121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC 181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA 241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT 301 GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC 361 CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC 421 TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT 481 GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT 541 CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCN GTCCCCCCTT 601 TTCTTTTAAA AAGTGNCTAA NANCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGNAN 661 CTGANGTGNG ANNGGAAAAN CNNNCTNCTN CTNCTCNTGN GCTNCTANCN NGGNNCNNTC 721 NGNANNNNNN NNNN //
Primer: SV40pro_F (Pr0110) Region: EM7 pro,Zeo Sequence file: RDB15444_B1K5g.seq
>15444_15444_B1K5_3_SV40pro_F_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNAN NNNTGNNGNN GCTTTTTTGG ANGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG NNCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC 121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA 181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG 241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG 301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT 361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC 421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG 481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA 541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGNTGGGCTT 601 CGGAATCGTT TTCCGGGANG CCGGCTGGAT GANCCTCCNG CGCGGGGNNC TCNTGCTGGA 661 NTTCTTCGCC CACCCCNNCT TGTTTATTGC AGCTTANANN GGTNACNAAT AAAGCNNNNN 721 NNTCNCNANN TNNNNNNAAN NNANNNNNTT TTTTTNNNN //
Primer: mRFP1-inner1 (Pr0443) Region: insert mid(mCherry 3', hCdt1(30_120) 5') Sequence file: RDB15444_B1K5h.seq
>15444_15444_B1K5_3_mRFP1-inner1_H01_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTCCTCCCT GNNNGGNNGN NCGNNTTCAT 61 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCNAN NGNCCCGTAA TGCANAAGAA 121 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG 181 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC 241 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT 301 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG 361 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC 421 GCTCGAGCCC AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA CCAGTGGNAN 481 CCGCAAGCGC GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CNNNNNNNGC CACNGGNNCN 541 NNNGAGACTG CGGCTGTCNN NGGNNNAGGT TTCCAGCCCC NGTACCCCCG ANGCCCCNGA 601 NATNNNNNNN CTGNNNCNTC TCCNGNNCNN AAANATANNN NNNNNCNNCC NNNGGNNNCN 661 NGTCNNNCCG NCCCNNNNTG ANATCCGCGN AGGNACNNNN AANACCNNCN CNNNGNNNNN 721 GCCNNNNNNN CNNNNNNNNG NNNNCNNNNN NNNCTNNNNN NNNNN //
Primer: AmCyan1-H_F (Pr1071) Region: insert mid(AmCyan1 3',hGem(1_110) 5') Sequence file: RDB15444_B1K5i.seq
>15444_15444_B1K5_3_AmCyan1-H_F_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NGGCNNCCNG NNCTCCNCCT TCNAGGTGAC CATGGCCAAC 61 GGCGGCCCCC TGGCCTTCTC CTTCGACATC CTGTCCACCG TGTTCATGTA CGGCAACCGC 121 TGCTTCACCG CCTACCCCAC CAGCATGCCC GACTACTTCA AGCAGGCCTT CCCCGACGGC 181 ATGTCCTACG AGAGAACCTT CACCTACGAG GACGGCGGCG TGGCCACCGC CAGCTGGGAG 241 ATCAGCCTGA AGGGCAACTG CTTCGAGCAC AAGTCCACCT TCCACGGCGT GAACTTCCCC 301 GCCGACGGCC CCGTGATGGC CAAGAAGACC ACCGGCTGGG ACCCCTCCTT CGAGAAGATG 361 ACCGTGTGCG ACGGCATCTT GAAGGGCGAC GTGACCGCCT TCCTGATGCT GCAGGGCGGC 421 GGCAACTACA GATGCCAGTT CCACACCTCC TACAAGACCA AGAAGCCCGT GACCATGCCC 481 CCCAACCACG TGGTGGAGCA CCGCATCGCC AGAACCGACC TGGACAAGGG CGGCAACAGC 541 GTGCAGCTGA CCGAGCACGC CGTGGCCCAC ATCACCTCCG TGGTGCCCTT CGGATATCCA 601 TCACACTGGC GGCCGCTCGA GANGAATCCC NGTATGAAGC ANNNNNAGAA NAANNCAAAN 661 AGAATANAAA GAATANTTCT GTCCNNNNAA NANNTCNNNN NNNGANTCNN CCNTCNNNNN 721 CNNNNNNNNN N //
Primer: EF1a-N_R (Pr0024) Region: EF1alpha pro,cPPT_CTS,RRE Sequence file: RDB15444_B1K5j.seq
>15444_15444_B1K5_3_EF1a-N_R_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNGTGC GCTCTGCCNN CTGACGGGCA CCGGNNCCTC 61 ACGAAGCTTG AATTAATTCA AAGTGAATTT CTGCTGTCCC TGTAATAAAC CCGAAAATTT 121 TGAATTTTTG TAATTTGTTT TTGTAATTCT TTAGTTTGTA TGTCTGTTGC TATTATGTCT 181 ACTATTCTTT CCCCTGCACT GTACCCCCCA ATCCCCCCTT TTCTTTTAAA ATTGTGGATG 241 AATACTGCCA TTTGTAGATC CGTTCACTAA TCGAATGGAT CTGTCTCTGT CTCTCTCTCC 301 ACCTTCTTCT TCTATTCCTT CGGGCCTGTC GGGTCCCCTC GGGGTTGGGA GGTGGGTCTG 361 AAACGATAAT GGTGAATATC CCTGCCTAAC TCTATTCACT ATAGAAAGTA CAGCAAAAAC 421 TATTCTTAAA CCTACCAAGC CTCCTACTAT CATTATGAAT AATTTTATAT ACCACAGCCA 481 ATTTGTTATG TTAAACCAAT TCCACAAACT TGCCCATTTA TCTAATTCCA ATAATTCTTG 541 TTCATTCTTT TCTTGCTGGT TTTGCGATTC TTCAATTAAG GAGTGTATTA AGCTTGTGTA 601 ATTGTTAATT TCTCTGTCCC ACTCCATCCA GGTCGTGTGA TTCCAAATCT GTTCCAGAGA 661 TTTATTACTC CAACTAGCAT TCCAAGGCAC AGCAGTGGTG CAAATGAGTT TTCCAGAGCA 721 ACCCCAAATC CCCAGGAGCT GTTGATCCTT TANGTATCTT TCCACAGCCA GGATTCTTGC 781 CTGGAGCTGC TTGATGCCCC AGACTGTGAG TTGCAACAGA TGCTGTTGCG CCTCAATANC 841 CCTCAGCAAA TTGTTCTGCT GCTGCACTAT ACCNNANAAT AATTGTCTGG NNNGTNCCGT 901 CNNNCNNCAN NGACNCNNCG CCNNTANNGC TTCCNGCTGC TCCCNANANC CNNNNGNANN 961 AAAGCNCCNA TTNCNNNNNN NNNN //
Primer: Cdt1_F1 (Pr0768) Region: insert mid(P2A, AmCyan1 5') Sequence file: RDB15444_B1K5k.seq
>15444_15444_B1K5_Cdt1_F1_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNGN NNNNNNNNNG NNNCCNTCAC CGGTCGCGCC NAGCGCGGCA 61 GCGGCGCCAC CAACTTCAGC CTGCTGAAGC AGGCCGGCGA CGTGGAGGAG AACCCCGGCC 121 CCCGCGAATT CGCCACCATG GCCCTGTCCA ACAAGTTCAT CGGCGACGAC ATGAAGATGA 181 CCTACCACAT GGACGGCTGC GTGAACGGCC ACTACTTCAC CGTGAAGGGC GAGGGCAGCG 241 GCAAGCCCTA CGAGGGCACC CAGACCTCCA CCTTCAAGGT GACCATGGCC AACGGCGGCC 301 CCCTGGCCTT CTCCTTCGAC ATCCTGTCCA CCGTGTTCAT GTACGGCAAC CGCTGCTTCA 361 CCGCCTACCC CACCAGCATG CCCGACTACT TCAAGCAGGC CTTCCCCGAC GGCATGTCCT 421 ACGAGAGAAC CTTCACCTAC GAGGACGGCG GCGTGGCCAC CGCCAGCTGG GAGATCAGCC 481 TGAAGGGCAA CTGCTTCGAG CACAAGTCCA CCTTCCACGG CGTGAACTTC CCCGCCGACG 541 GCCCCGTGAT GGCCAAGAAG ACCACCGGCT GGGACCCCTC CTTCGAGAAG ATGACCGTGT 601 GCGACGGCAT CTTGAAGGGC GACGTGACCG CCTTCCTGAT GCTGCAGGGC GGCGGCAACT 661 ACAGATGCCA GTTCCACACC TCCTACAAGA CCAAGAAGCC CGTGACCATG CCCCCCAACC 721 ACGTGGTGGA GCACCGCATC GCCAGAACCG ACCTGGACAA GGGCGGCAAC AGCGTGCAGC 781 TGANCGANCA CGCCGTGNCC CACATCACCT CCGNGGTGNN CTTCNGANAT CCATCACACT 841 GGCGGCCGCT CNANATGAAT CCCAGTATGA AGCANAAACN AGAAGAAATC NAAGANAATA 901 TNAAGAANAG TTCTGTCCNN ANAAGAANTC TNNNNNNNNN TCANCNNNCT GCNNCNNNNT 961 NNNNNNNNNG NNGANAAAAN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]

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External Database
Human CDT1

Human Geminin

Discosoma sp. RFP

Anemonia majano CFP

Reference sequence