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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(SA)2.1/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15444
Resource name tFucci(SA)2.1/pCSII-EF
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Anemonia majano CFP cDNA
Reference of nucleotide sequence LC192887 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering Please visit Ordering instruction.[link] UCSD's MTA is also required. Please visit Additional Forms section.
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Exclusive MTA (for use for not-for-profit academic purpose)[Link]
Remarks Remember that you will be working with samples containing infectious virus.
UCSD's MTA is also required. Please visit Additional Forms section.
The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs).
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15444 tFucci(SA)2.1/pCSII-EF DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15444_A7Jvp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_R
Sequence file: RDB15444_A7Jva.seq check
>D05018A4_1_A7Jv_mCherry-C_R_A01_01_ABI24.ab1
    1 GTTGGAAGTA TGTCCACTTG ATGTTGACGT TGTAGGCGCC GGGCAGCTGC ACGGGCTTCT
   61 TGGCCTTGTA GGTGGTCTTG ACCTCAGCGT CGTAGTGGCC GCCGTCCTTC AGCTTCAGCC
  121 TCTGCTTGAT CTCGCCCTTC AGGGCGCCGT CCTCGGGGTA CATCCGCTCG GAGGAGGCCT
  181 CCCAGCCCAT GGTCTTCTTC TGCATTACGG GGCCGTCGGA GGGGAAGTTG GTGCCGCGCA
  241 GCTTCACCTT GTAGATGAAC TCGCCGTCCT GCAGGGAGGA GTCCTGGGTC ACGGTCACCA
  301 CGCCGCCGTC CTCGAAGTTC ATCACGCGCT CCCACTTGAA GCCCTCGGGG AAGGACAGCT
  361 TCAAGTAGTC GGGGATGTCG GCGGGGTGCT TCACGTAGGC CTTGGAGCCG TACATGAACT
  421 GAGGGGACAG GATGTCCCAG GCGAAGGGCA GGGGGCCACC CTTGGTCACC TTCAGCTTGG
  481 CGGTCTGGGT GCCCTCGTAG GGGCGGCCCT CGCCCTCGCC CTCGATCTCG AACTCGTGGC
  541 CGTTCACGGA GCCCTCCATG TGCACCTTGA AGCGCATGAA CTCCTTGATG ATGGCCATGT
  601 TATCCTCCTC GCCCTTGCTC ACCATGGTGG ATCCGCGGCC GCTGCAGAAT TCTCGAGACC
  661 GGTAGCGTGT TCACGACACC TGAAATGGAA GAAAAAAACT TTGAACCACT GTCTGAGGCT
  721 TGAGAATGAA CCAAGATCCA AACTCAAAAA GGGCAAATTC CAAGGAGAAT TACATCAAGT
  781 GCCAAGCTGG CCTAACTTCA GTCTCCACCC ACTCAGTGTG GGGAAACTCC ATCGCATAAA
  841 ACCCCTCCCC CCCAACCTAA AGACGACGTA CTCCAAAAGC TCCAGAACTA ATCGAGGTGC
  901 CTGGACGGCG CCCGGTACTC CGTGGAGTCA CATGAAGCGA CGGCTGAGGA CGGAAAGGCC
  961 CCTTTCCTTT GTGTGGGTGA CTCACCCGCC CGCTCTCCCG AGCGCCGCGT CCCTCCATTT
 1021 TTGAGCCCCC TGGAGCAGGA CCGGGAAAGC CGGCTCATCT TTCGCTCACG CCACTGTTGG
1081 CCGGAACGGG CCAGCCCCTT TGACTCGCTC CTAAGGGGGC CGGGGGGATC GA
//
Primer: mCherry-C_F
Sequence file: RDB15444_A7Jvb.seq check
>D05018A4_1_A7Jv_mCherry-C_F_F12_18_ABI24.ab1
    1 TGTCAGGTAG GCCGTGCAGC TGCCCGGCGC CTACAACGTC AACATCAAGT TGGACATCAC
   61 CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC
  121 CACCGGCGGC ATGGACGAGC TGTACAAGGG ATATCCATCA CACTGGCGGC CGCTCGAGCC
  181 CAGCCCCGCC AGGCCCGCAC TCCGCGCCCC GGCCTCCGCT ACCAGTGGCA GCCGCAAGCG
  241 CGCCCGCCCG CCCGCCGCCC CCGGACGCGA CCAGGCCAGG CCACCGGCCC GCAGGAGACT
  301 GCGGCTGTCG GTGGACGAGG TTTCCAGCCC CAGTACCCCC GAGGCCCCAG ACATCCCAGC
  361 CTGCCCTTCT CCGGGCCAGA AGATAAAGAA ATCCACCCCG GCAGCAGGTC AGCCGCCCCA
  421 CCTGACATCC GCGCAGGACC AGGACACCAT CACCGGTCGC GCCAAGCGCG GCAGCGGCGC
  481 CACCAACTTC AGCCTGCTGA AGCAGGCCGG CGACGTGGAG GAGAACCCCG GCCCCCGCGA
  541 ATTCGCCACC ATGGCCCTGT CCAACAAGTT CATCGGCGAC GACATGAAGA TGACCTACCA
  601 CATGGACGGC TGCGTGAACG GCCACTACTT CACCGTGAAG GGCGAGGGCA GCGGCAAGCC
  661 CTACGAGGGC ACCCAGACCT CCACCTTCAA GGTGACCATG GCCAACGGCG GCCCCCTGGC
  721 CTTCTCCTTC GACATCCTGT CCACCGTGTT CATGTACGGC AACCGCTGCT TCACCGCCTA
  781 CCCCACCAGC ATGCCCGACT ACTTCAAGCA GGCCTTCCCC GACGGCATGT CCTACGAGAG
  841 AACCTTCACC TACGAGGACG GCGGCGTGGC CACCGCCAGC TGGGAGATCA GCCTGAAGGG
  901 CAACTGCTTC GAGCACAAGT CCACCCTTCC ACGGCGTGAA CTTCCCCGCC GACGGGCCCC
  961 GTGATGGCCA AGAAGACCAC CGGCTGGGAC CCCTCCTTCG AGAAGATGAC CGTGTGCGAC
 1021 GCATCTTGAA GGGCGACGTG ACGCTTCTTG ATGCTGCAGG CGGCGCACCT ACAGGATGGC
 1081 AGGTCCACAC TTCCTTACAG ACAAGAAGTC AGTGACCATG GCTGCATCAG TGTGAAGCAA
1141 TCGGTCCAT
//
Primer: PRE_rev
Sequence file: RDB15444_A7Jvc.seq check
>D05018A4_1_A7Jv_PRE_rev_B01_04_ABI24.ab1
    1 ACGCATCACA TAGCGTAAAG GAGCAACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
   61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGATTAC
  121 AGCGCCTTTC TCCGTTTTTC TGCCACTTCC TTCCAATACT GAGAGGATGG ATTTTCTTTA
  181 ATCATAAGAT CAAATGACTC CTGGGTGACT CCTCCAAGAT TTTTATTTTC ACTAGATTCT
  241 GGGACAATAA CCCCAGGGCT GGAAGTTGTA GATGTTAAGT GGTCATTCCG ATGTTTCCTT
  301 TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA TGCAGAAGGC
  361 TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC TTTGATTTCT
  421 TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA TGGATATCCG
  481 AAGGGCACCA CGGAGGTGAT GTGGGCCACG GCGTGCTCGG TCAGCTGCAC GCTGTTGCCG
  541 CCCTTGTCCA GGTCGGTTCT GGCGATGCGG TGCTCCACCA CGTGGTTGGG GGGCATGGTC
  601 ACGGGCTTCT TGGTCTTGTA GGAGGTGTGG AACTGGCATC TGTAGTTGCC GCCGCCCTGC
  661 AGCATCAGGA AGGCGGTCAC GTCGCCCTTC AAGATGCCGT CGCACACGGT CATCTTCTCG
  721 AAGGAGGGGT CCCAGCCGGT GGTCTTCTTG GCCATCACGG GGCCGTCGGC GGGGAAGTTC
  781 ACGCCGTGGA AGGTGGACTT GTGCTCGAAG CAGTTGCCCT TCAGGCTGAT CTCCCAGCTG
  841 GCGGTGGCCA CGCCGCCGTC CTCGTAGGTG AAGGTTCTCT CGTAGGACAT GCCGTCGGGG
  901 AAAGGCCTGC TTGAAGTAGT CGGGCATGCT GGTGGGGTAG GCGGTGAAGC AGCGGTTGCC
  961 GTACATGAAC ACGGTGGACA GGATGTCGAA AGGAGAAGGC CAGGGGGGCC GCCGTTGGGC
 1021 CATGGTCACC TTGAAGTGAG TCTGGTGCCC TCGTAGGCTG CCGCTGCCCT CGCCTTCACG
 1081 TTGAGTAGTT GATGATCACG CAGGCGTTCC AATGTGTAAG TCAATCCTCC ATGTCGTTCG
1141 CCGGATGAAA CTTTGATGGA ACAGGGGCA
//
Primer: HIVgp_R1
Sequence file: RDB15444_A7Jvd.seq check
>D05018A4_1_A7Jv_HIVgq_R1_G12_21_ABI24.ab1
    1 CCGGAGGTTA ATATTTCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG
   61 GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC
  121 CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCCTTG CTGTGCGGTG
  181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC
  241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT
  301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT
  361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA
  421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT
  481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG
  541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT
  601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC
  661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
  721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
  781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
  841 CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC CCACCGTACA
  901 CGCCTACCGC CCATTTGCGT CAATGGGGCG GAGTTTGTTA CGACATTTTG GAAAGTCCCG
  961 TTGATTTTGG GTGCCCAAAA CAAACTCCCA ATTGACGTCA ATGGGGTGGA GACTTGGAAA
 1021 TCCCCGTGAG TCAAACCGCT ATCCACGCCC ATTTGATGTA CTGCCCAAAA CCGCATTCAC
 1081 ATGTTTAATA GCGAATGACT TATACGTAGA TGTAACTTGC TAAGGTTAGA AGTTTCCCCA
1141 TAAGGTCTCA ATGTGTTACT TGGGC
//
Primer: BGH_rev2
Sequence file: RDB15444_A7Jve.seq check
>D05018A4_1_A7Jv_BGH_rev2_D01_10_ABI24.ab1
    1 CCCATTTTGG GGGTTCAGGT AAGGGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC
   61 TAGAAGGCAC AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC
  121 ACTGACTAAA AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC
  181 ACTACTTGAA GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG
  241 CCAGAGAGCT CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC
  301 AGCTCTCGGG CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT
  361 CTCTCCGGGT CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT
  421 TCATTGGCTT CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG
  481 GTCAGTGGAT ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT
  541 TGTGTGTGGT AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT
  601 TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT
  661 GGTCTTAAAG GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT
  721 AGCCAGGCAC AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT
  781 TCTAGGTCTC GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA
  841 GGGCGAAGGC GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG
  901 TCCGCTGGAT TGAGGGCCGA AGGGACGTAG CAGAAGGACG TCCCGCGCAG AATCCAGGTG
  961 GCAACACAGG CGAGCAGCCA TGGAAAGGAC GTCAGCTTCC CCGACAACAC CACGGAAATT
 1021 GTCAGTGCCC ACAGCGAGCC CCTGTCCAGC AGCGGGCAAA GGCAAGCGGC GATGAGTTCC
 1081 GCCGTGGCAA TAGGAGGGGA AGCGAAGTTC CGGAAAGAAC TGACAGTGTG CAATGCCCAA
1141 CAGTGGGGTT GCGTTCAGCA AAACACAC
//
Primer: SV40pro_F
Sequence file: RDB15444_A7Jvf.seq check
>D05018A4_1_A7Jv_SV40pro_F_E01_13_ABI24.ab1
    1 CCTTGGTCCG TAACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
  121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
  181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
  241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
  301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
  361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
  421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
  481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
  541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
  661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
  721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
  781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
  841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
  901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGGTGCC TAATGAGTGA GCTAACTCAC
  961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
 1021 TTAATGAATC GGGCCAACGC GCGGGGAGAG GCGGATTGCG TATTGGCGCT CTTCCGCTTT
 1081 CCTCGCTCAC TGACTCGCTG CGCTCGGGTC GGTCCGCTGC CGCCGAGCCG GCTATCAGCC
1141 TCCACCCTCA AAGGGCGGGA TAAAATACCG GTTTTAATTC A
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl