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tFucci(SA)2.1/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15444
Resource name tFucci(SA)2.1/pCSII-EF
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Anemonia majano CFP cDNA
Reference of insert sequence LC192887 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Anemonia majano CFP

        Reference sequence
          
        Lentiviral plasmid list. [link]

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Ordering instruction.[link] 
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        This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        提供依頼書 [Word]
        Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
        Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15444 tFucci(SA)2.1/pCSII-EF DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15444_B1K5p1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: EF1a-C_F
        Region: EF1alpha pro,insert 5'(mCherry 5')
        Sequence file: RDB15444_B1K5a.seq check
        >15444_15444_B1K5_3_EF1a-C_F_A01_01_ABI24.ab1
            1 NNNNNNNNNN NNNTTTTGAN TTTGGNNCTT GGTTNNTTCT CAAGCCTCAG ACAGTGGTTC
           61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAACACGCTA CCGGTCTCGA GAATTCTGCA
          121 GCGGCCGCGG ATCCACCATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG
          181 AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG
          241 AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA
          301 AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA
          361 AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG
          421 GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG
          481 ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC
          541 CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGANGCCTCC TCCGAGCGGA
          601 TGTACCCCGA GGACGGCGCC CTGAANGGCG AGNTCAAGCA GANGCTGAAG CTGNNGACGG
          661 CGGCCACTAC GACGCTGANG NCNAGANNNC CTACNNNNCC NAGANNCCNN TGNAGCTGCC
        721 NNGNGCCNNN NNNN
        //
        Primer: PRE_rev
        Region: PRE,insert 3'(hGem(1_110), AmCyan1 3')
        Sequence file: RDB15444_B1K5b.seq check
        >15444_15444_B1K5_3_PRE_rev_B01_04_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN GNNCANCATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
           61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGATTAC
          121 AGCGCCTTTC TCCGTTTTTC TGCCACTTCC TTCCAATACT GAGAGGATGG ATTTTCTTTA
          181 ATCATAAGAT CAAATGACTC CTGGGTGACT CCTCCAAGAT TTTTATTTTC ACTAGATTCT
          241 GGGACAATAA CCCCAGGGCT GGAAGTTGTA GATGTTAAGT GGTCATTCCG ATGTTTCCTT
          301 TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA TGCAGAAGGC
          361 TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC TTTGATTTCT
          421 TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA TGGATATCCG
          481 AAGGGCACCA CGGAGGTGAT GTGGGCCACG GCGTGCTCGG TCAGCTGCAC GCTGTTGCCG
          541 CCCTTGTCCA GGTCGGTTCT GGCGATGCGG TGCTCCACCA CGTGGTTGGG GGGCATGGTC
          601 ACGGGCTTCT TGGTCTTGTA NGANGTGTGG AACTGGCATC TGTAGTTGCC GCCGCCCTGC
          661 AGCATCANGA ANGNGGNCNC GTCGCCCNTC NNNANGNCGT CNCANNCNGT CATCNNCTCN
        721 ANNNNNGGGN NCNNNNNN
        //
        Primer: mCherry-C_F
        Region: insert mid(mCherry 3', hCdt1(30_120), P2A, AmCyan1 5')
        Sequence file: RDB15444_B1K5c.seq check
        >15444_15444_B1K5_3_mCherry-C_F_C01_07_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN CCNNCCCCNG NCAGGCCCGC ANTCCGCGCC CCGGCCTCCG
           61 CTAACCAGTG GCAGCCGCAA GCGCGCCCGC CCGCCCGCCG CCCCCGGACG CGACCAGGCC
          121 AGGCCACCGG CCCGCAGGAG ACTGCGGCTG TCNGTGGACN AGGTTTCCAG CCCCAGTACC
          181 CCCGAGGCCC CAGACATCCC AGCCTGCCCT TCTCCGGGCC AGAAGATAAA GAAATCCACC
          241 CCGGCAGCAG GTCAGCCGCC CCACCTGACA TCCGCGCAGG ACCAGGACAC CATCACCGGT
          301 CGCGCCAAGC GCGGCAGCGG CGCCACCAAC TTCAGCCTGC TGAAGCAGGC CGGCGACGTG
          361 GAGGAGAACC CCGGCCCCCG CGAATTCGCC ACCATGGCCC TGTCCAACNN NTTCATCGGC
          421 GACGACATGA AGATGACCTA CCACATGNNN NGCTGCGTGA ACGGCCACTA CTTCACCGTG
          481 AAGGGCGAGG GCAGCGGCAA GCCCTACGAG GGCACCCANA CCTCCACCTT CAAGGTGACC
          541 ATGGCCAACG GCGGCCCCCT GGCCTTCTCC TTCGACATCC TGTCCACCGT GTTCATGTAC
          601 NGCAACCGCT GCTTCACCGN CTACNCCNNN CAGCATGNCC GACNACTTCA AGCANGCCTT
          661 CCCCGACNGN ATGTCCTACG AGNNANNCNT TNACCTACNA GGANNGNNGG CGNNNNCANN
          721 GNCAGNNNGN NNATCAGCCT GNNNNNNNNN GCNNNNNGCA NNNGTCNCCT TCNNNNNCGN
        781 GNNCTTCCCN NNCGANGNNN NNNNNNN
        //
        Primer: CMV_Forward
        Region: CMV pro,5'LTR,HIV-1 psi
        Sequence file: RDB15444_B1K5d.seq check
        >15444_15444_B1K5_3_CMV-Forward_D01_10_ABI24.ab1
            1 NNNNNNNNNN NNNNNTTGNN NCNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
           61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
          121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
          181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
          241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
          301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
          361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
          421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
          481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
          541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
          601 TGGGACAGCT ACNACCNTCC CTTCNGACAG GNTCAGAAGA ACTTAGATCA TTATATAATA
          661 CAGTAGCNAC CCTCNANNGT GTGCATCAAN NNANAGAGAN AAANNNNNNN NANGNANNCN
        721 TNNNNNNNNN N
        //
        Primer: HIVgp_R1
        Region: HIV-1 psi
        Sequence file: RDB15444_B1K5e.seq check
        >15444_15444_B1K5_3_HIVgp_R1_E01_13_ABI24.ab1
            1 NNNNNNNNNN NNNNNCTCTT TTTTCTCTCT GCACCACTCT TCTCTTTGCC TTGGTGGGTG
           61 CTACTCCTAA TGGTTCAATT TTTACTACTT TATATTTATA TAATTCACTT CTCCAATTGT
          121 CCCTCATATC TCCTCCTCCA GGTCTGAAGA TCAGCGGCCC TTGCTGTGCG GTGGTCTTAC
          181 TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT ATCTCTATCC
          241 TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA TGATCTAAGT TCTTCTGATC
          301 CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT ACAGCCTTCT GATGTTTCTA
          361 ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT TGCCCATACT ATATGTTTTA
          421 ATTTATATTT TTTCTTTCCC CCTGGCCTTA ACCGAATTTT TTCCCATCGC GATCTAATTC
          481 TCCCCCGCTT AATACTGACG CTCTCGCACC CATCTCTCTC CTTCTAGCCT CCGCTAGTCA
          541 AAATTTTTGG CGTACTCACC AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC GCTTCAGCAA
          601 GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT CGCTTTCNAG TCCCTGTTCG
          661 GGCGCCACTG CTAGAGANTT TCCNCACTGN CNNAANNNNN CTNANGGNNC TCTNGTNNNN
        721 NGANTNNNNN NNNNN
        //
        Primer: BGH_rev3
        Region: bGH pA,3'LTR
        Sequence file: RDB15444_B1K5f.seq check
        >15444_15444_B1K5_3_bGH_rev3_F01_16_ABI24.ab1
            1 NNNNNNNNNN NNNNGGNNGN NGGGCANACA ACAGATGGCT GGCAACTAGA AGGCACAGTC
           61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG
          121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC
          181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA
          241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT
          301 GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC
          361 CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC
          421 TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT
          481 GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT
          541 CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCN GTCCCCCCTT
          601 TTCTTTTAAA AAGTGNCTAA NANCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGNAN
          661 CTGANGTGNG ANNGGAAAAN CNNNCTNCTN CTNCTCNTGN GCTNCTANCN NGGNNCNNTC
        721 NGNANNNNNN NNNN
        //
        Primer: SV40pro_F
        Region: EM7 pro,Zeo
        Sequence file: RDB15444_B1K5g.seq check
        >15444_15444_B1K5_3_SV40pro_F_G01_19_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNAN NNNTGNNGNN GCTTTTTTGG ANGCCTAGGC TTTTGCAAAA
           61 AGCTCCCGGG NNCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
          121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
          181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
          241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
          301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
          361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
          421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
          481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
          541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGNTGGGCTT
          601 CGGAATCGTT TTCCGGGANG CCGGCTGGAT GANCCTCCNG CGCGGGGNNC TCNTGCTGGA
          661 NTTCTTCGCC CACCCCNNCT TGTTTATTGC AGCTTANANN GGTNACNAAT AAAGCNNNNN
        721 NNTCNCNANN TNNNNNNAAN NNANNNNNTT TTTTTNNNN
        //
        Primer: mRFP1-inner1
        Region: insert mid(mCherry 3', hCdt1(30_120) 5')
        Sequence file: RDB15444_B1K5h.seq check
        >15444_15444_B1K5_3_mRFP1-inner1_H01_22_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTCCTCCCT GNNNGGNNGN NCGNNTTCAT
           61 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCNAN NGNCCCGTAA TGCANAAGAA
          121 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG
          181 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC
          241 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT
          301 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG
          361 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC
          421 GCTCGAGCCC AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA CCAGTGGNAN
          481 CCGCAAGCGC GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CNNNNNNNGC CACNGGNNCN
          541 NNNGAGACTG CGGCTGTCNN NGGNNNAGGT TTCCAGCCCC NGTACCCCCG ANGCCCCNGA
          601 NATNNNNNNN CTGNNNCNTC TCCNGNNCNN AAANATANNN NNNNNCNNCC NNNGGNNNCN
          661 NGTCNNNCCG NCCCNNNNTG ANATCCGCGN AGGNACNNNN AANACCNNCN CNNNGNNNNN
        721 GCCNNNNNNN CNNNNNNNNG NNNNCNNNNN NNNCTNNNNN NNNNN
        //
        Primer: AmCyan1-H_F
        Region: insert mid(AmCyan1 3',hGem(1_110) 5')
        Sequence file: RDB15444_B1K5i.seq check
        >15444_15444_B1K5_3_AmCyan1-H_F_A02_02_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NGGCNNCCNG NNCTCCNCCT TCNAGGTGAC CATGGCCAAC
           61 GGCGGCCCCC TGGCCTTCTC CTTCGACATC CTGTCCACCG TGTTCATGTA CGGCAACCGC
          121 TGCTTCACCG CCTACCCCAC CAGCATGCCC GACTACTTCA AGCAGGCCTT CCCCGACGGC
          181 ATGTCCTACG AGAGAACCTT CACCTACGAG GACGGCGGCG TGGCCACCGC CAGCTGGGAG
          241 ATCAGCCTGA AGGGCAACTG CTTCGAGCAC AAGTCCACCT TCCACGGCGT GAACTTCCCC
          301 GCCGACGGCC CCGTGATGGC CAAGAAGACC ACCGGCTGGG ACCCCTCCTT CGAGAAGATG
          361 ACCGTGTGCG ACGGCATCTT GAAGGGCGAC GTGACCGCCT TCCTGATGCT GCAGGGCGGC
          421 GGCAACTACA GATGCCAGTT CCACACCTCC TACAAGACCA AGAAGCCCGT GACCATGCCC
          481 CCCAACCACG TGGTGGAGCA CCGCATCGCC AGAACCGACC TGGACAAGGG CGGCAACAGC
          541 GTGCAGCTGA CCGAGCACGC CGTGGCCCAC ATCACCTCCG TGGTGCCCTT CGGATATCCA
          601 TCACACTGGC GGCCGCTCGA GANGAATCCC NGTATGAAGC ANNNNNAGAA NAANNCAAAN
          661 AGAATANAAA GAATANTTCT GTCCNNNNAA NANNTCNNNN NNNGANTCNN CCNTCNNNNN
        721 CNNNNNNNNN N
        //
        Primer: EF1a-N_R
        Region: EF1alpha pro,cPPT_CTS,RRE
        Sequence file: RDB15444_B1K5j.seq check
        >15444_15444_B1K5_3_EF1a-N_R_B01_04_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNGTGC GCTCTGCCNN CTGACGGGCA CCGGNNCCTC
           61 ACGAAGCTTG AATTAATTCA AAGTGAATTT CTGCTGTCCC TGTAATAAAC CCGAAAATTT
          121 TGAATTTTTG TAATTTGTTT TTGTAATTCT TTAGTTTGTA TGTCTGTTGC TATTATGTCT
          181 ACTATTCTTT CCCCTGCACT GTACCCCCCA ATCCCCCCTT TTCTTTTAAA ATTGTGGATG
          241 AATACTGCCA TTTGTAGATC CGTTCACTAA TCGAATGGAT CTGTCTCTGT CTCTCTCTCC
          301 ACCTTCTTCT TCTATTCCTT CGGGCCTGTC GGGTCCCCTC GGGGTTGGGA GGTGGGTCTG
          361 AAACGATAAT GGTGAATATC CCTGCCTAAC TCTATTCACT ATAGAAAGTA CAGCAAAAAC
          421 TATTCTTAAA CCTACCAAGC CTCCTACTAT CATTATGAAT AATTTTATAT ACCACAGCCA
          481 ATTTGTTATG TTAAACCAAT TCCACAAACT TGCCCATTTA TCTAATTCCA ATAATTCTTG
          541 TTCATTCTTT TCTTGCTGGT TTTGCGATTC TTCAATTAAG GAGTGTATTA AGCTTGTGTA
          601 ATTGTTAATT TCTCTGTCCC ACTCCATCCA GGTCGTGTGA TTCCAAATCT GTTCCAGAGA
          661 TTTATTACTC CAACTAGCAT TCCAAGGCAC AGCAGTGGTG CAAATGAGTT TTCCAGAGCA
          721 ACCCCAAATC CCCAGGAGCT GTTGATCCTT TANGTATCTT TCCACAGCCA GGATTCTTGC
          781 CTGGAGCTGC TTGATGCCCC AGACTGTGAG TTGCAACAGA TGCTGTTGCG CCTCAATANC
          841 CCTCAGCAAA TTGTTCTGCT GCTGCACTAT ACCNNANAAT AATTGTCTGG NNNGTNCCGT
          901 CNNNCNNCAN NGACNCNNCG CCNNTANNGC TTCCNGCTGC TCCCNANANC CNNNNGNANN
        961 AAAGCNCCNA TTNCNNNNNN NNNN
        //
        Primer: Cdt1_F1
        Region: insert mid(P2A, AmCyan1 5')
        Sequence file: RDB15444_B1K5k.seq check
        >15444_15444_B1K5_Cdt1_F1_C01_03_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNGN NNNNNNNNNG NNNCCNTCAC CGGTCGCGCC NAGCGCGGCA
           61 GCGGCGCCAC CAACTTCAGC CTGCTGAAGC AGGCCGGCGA CGTGGAGGAG AACCCCGGCC
          121 CCCGCGAATT CGCCACCATG GCCCTGTCCA ACAAGTTCAT CGGCGACGAC ATGAAGATGA
          181 CCTACCACAT GGACGGCTGC GTGAACGGCC ACTACTTCAC CGTGAAGGGC GAGGGCAGCG
          241 GCAAGCCCTA CGAGGGCACC CAGACCTCCA CCTTCAAGGT GACCATGGCC AACGGCGGCC
          301 CCCTGGCCTT CTCCTTCGAC ATCCTGTCCA CCGTGTTCAT GTACGGCAAC CGCTGCTTCA
          361 CCGCCTACCC CACCAGCATG CCCGACTACT TCAAGCAGGC CTTCCCCGAC GGCATGTCCT
          421 ACGAGAGAAC CTTCACCTAC GAGGACGGCG GCGTGGCCAC CGCCAGCTGG GAGATCAGCC
          481 TGAAGGGCAA CTGCTTCGAG CACAAGTCCA CCTTCCACGG CGTGAACTTC CCCGCCGACG
          541 GCCCCGTGAT GGCCAAGAAG ACCACCGGCT GGGACCCCTC CTTCGAGAAG ATGACCGTGT
          601 GCGACGGCAT CTTGAAGGGC GACGTGACCG CCTTCCTGAT GCTGCAGGGC GGCGGCAACT
          661 ACAGATGCCA GTTCCACACC TCCTACAAGA CCAAGAAGCC CGTGACCATG CCCCCCAACC
          721 ACGTGGTGGA GCACCGCATC GCCAGAACCG ACCTGGACAA GGGCGGCAAC AGCGTGCAGC
          781 TGANCGANCA CGCCGTGNCC CACATCACCT CCGNGGTGNN CTTCNGANAT CCATCACACT
          841 GGCGGCCGCT CNANATGAAT CCCAGTATGA AGCANAAACN AGAAGAAATC NAAGANAATA
          901 TNAAGAANAG TTCTGTCCNN ANAAGAANTC TNNNNNNNNN TCANCNNNCT GCNNCNNNNT
        961 NNNNNNNNNG NNGANAAAAN NNNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl