Resource data sheet
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GEPRA-G/ pT2KXIGdeltain

Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish.

Catalog number RDB15253
Resource name GEPRA-G/ pT2KXIGdeltain
Clone info. Expression vector of genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-gamma. For generation of transgenic zebrafish.
Vector backbone pT2KXIG deltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Gene/insert name mouse RARgamma cDNA Aequorea victoria GFP cDNA
Depositor Miyawaki, Atsushi |
 
Other clones in our bank mouse Rarg (NCBI Gene 19411) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering Please visit Information of Request for Distribution. 
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Schedule A (GFP-A) [Link]
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Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of the depositor.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15253 GEPRA-G/ pT2KXIGdeltain DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Sensor and Visualization (English text)

References

original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15253_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F2 RDB15253_A7H9a.seq
>D04498G2-1_A7H9_EF1a-C_F2_G10_19_ABI24.ab1
    1 GTTGGGGTGT AAGGCCCGGT TCGCTCTCTT CCTCACCGGG TCTGCGGCGA GTTCTAGCTG
   61 AAGCTTCCTG CAGGTCGACC GATCCTGAGA ACTTCAGGGT GAGTTTGGGG ACCCTTGATT
  121 GTTCTTTCTT TTTCGCTATT GTAAAATTCA TGTTATATGG AGGGGGCAAA GTTTTCAGGG
  181 TGTTGTTTAG AATGGGAAGA TGTCCCTTGT ATCACCATGG ACCCTCATGA TAATTTTGTT
  241 TCTTTCACTT TCTACTCTGT TGACAACCAT TGTCTCCTCT TATTTTCTTT TCATTTTCTG
  301 TAACTTTTTC GTTAAACTTT AGCTTGCATT TGTAACGAAT TTTTAAATTC ACTTTTGTTT
  361 ATTTGTCAGA TTGTAAGTAC TTTCTCTAAT CACTTTTTTT TCAAGGCAAT CAGGGTATAT
  421 TATATTGTAC TTCAGCACAG TTTTAGAGAA CAATTGTTAT AATTAAATGA TAAGGTAGAA
  481 TATTTCTGCA TATAAATTCT GGCTGGCGTG GAAATATTCT TATTGGTAGA AACAACTACA
  541 CCCTGGTCAT CATCCTGCCT TTCTCTTTAT GGTTACAATG ATATACACTG TTTGAGATGA
  601 GGATAAAATA CTCTGAGTCC AAACCGGGCC CCTCTGCTAA CCATGTTCAT GCCTTCTTCT
  661 CTTTCCTACA GCTCCTGGGC AACGTGCTGG TTGTTGTGCT GTCTCATCAT TTTGGCAAAG
  721 AATTCCTCGA CGGATCCCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC
  781 ATCCTGGTCG AGCTGCAGGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC
  841 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG
  901 CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGAGCTGGG GCGTGCAGTG CTTCAGCCGC
  961 TACCCCGACC ACATGAAAGC AGCACGACTT TCTTCAAGTC CGTCATGCCC GAATGCTACG
 1021 TCCAGGAGCG CACCAATCTT CTTCAGGACG ACGGCACTAC AAGACCGGCG CGAGTGAGGT
1081 TCGAAGGCGA CACCTGGTGG ATCGCAATCG AGCTGGAAAG GGCCATCCGA ACCTT
//
Primer: pAxCALNL_F1 RDB15253_A7H9b.seq
>D04498G2_1_A7H9_pAxCALNLF1_D01_04_ABI08.ab1
    1 GAAAGGGTAT CTTATCATGT CTGGATCATC ATCGATGAAT TAATTCGAGC TCCACCGCGG
   61 TGGCGGCCGC CAGTGTGATG GATATCTGCA GAATTCTTAG TCCTCGATGT TGTGGCGGAT
  121 CTTGAAGTTG GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT
  181 GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC
  241 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT
  301 GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC
  361 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC
  421 GTAGCCCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAG
  481 CAGCTTCAGG GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA
  541 CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG
  601 CTCCTCGCCC TTGCTCACCA TGCCACCGGT ACCGCCCTTG TACAGCTCGT TCATGCCTTC
  661 AGTGATCCCG GCGGCGGTCA GGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG
  721 GTCTTTGAAC AGGGCGGACT GGTAGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC
  781 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCCGC CCATGAGCTC
  841 CTCAAACATC TCCGGGTTCT CCAGCATCTC TCGGATCAGG GGTGGCATCG GGCCTGGAAT
  901 CTCCATCTTC AGGGTTATAG CCCTTTCTGC TCCCTTAGTG CTGATGCCCC GGAGGTCGGG
  961 TGATTTTCAT CAGCATCCTT GGGAACATGT AGGGTTGGCT GGGTCTCCGG TCGCCGGGCA
 1021 ATAGAGCCCT CAGGCTTTCC AAGCAGGGGC CTCCTGCAGC TTGTCATCTT CTCGGGCTTC
 1081 TTCAGGTCCA TCGGTCTTCC ACAGAATTGA GCCAGATAGC ACTTAGTTAG CTCCAGTCTC
1141 GTGATCATTC CATTTCTTCC AAGCG
//
Primer: RARgamma_LBD RDB15253_A7H9c.seq
>D04498G2_1_A7H9_RARgamma_LBD_F01_06_ABI08.ab1
    1 CTGAATAGGG GGTGGCATCG GGGGCCTGGG AATCTCCATC TTCAGGGTTA TAGCCCTTTC
   61 TGCTCCCTTA GTGCTGATGC CCCGGAGGTC GGTGATTTTC ATCAGCATCC TTGGGAACAT
  121 GTAGGGTTGG CTGGGTCTCC GTCGCCGGGC ATAGAGCCTC AGGGCTTCCA GCAGGGGCTC
  181 CTGCAGCTTG TCCACCTTCT CGGGCTCTTC CAGGTCCATT CGGTCTCCAC AGATGAGGCA
  241 GATAGCACTA AGTAGCCCAG TCTCGGTGTC ATCCATCTCC AGGGGCAGCA GCTGCCCGGC
  301 AAAGGCAAAG ACGAGGTCTG TAAGGGGCCC AAAGCCAGCA TTGTGCATCT GGGTTCGGTT
  361 CAGGGTCAGC CCATCCGAGA ATGTCATAGT GTCCTGCTCT GGGGTATACC TTGTACAGAT
  421 CCGCAGCATT AGGATGTCCA GACAAGCAGC CTTGAGCAGC GTGATCTGGT CGGCAATGCT
  481 GAGCCCTGTA AAACCAGGCA GCCGCTTCGC AAACTCCACA ATCTTGATGA TGCATTTGGT
  541 GGCCAGCTCG CTGAACTTGT CCCACAGCCC CAGGTCCAGC TGCACCCGGT GATCTGCACT
  601 GGAGTTCGTG GTGTACTTGC CCAGCTGGCA GAGTGAGGGA AAAGTCTCCT GGTGGGCTTT
  661 GCTGACCTTG GTGATGAGTT CCTCTAACTG TGGACTCAGT TCATAGCTGT CATGCATGCG
  721 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT
  781 CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGG TTCACGGGGC CGTCGCCGAT
  841 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCACGA TGTTGTGGCG
  901 GATCTTGAAG TTTGGCCTTG ATGCCGTTCT TCTGCTTGTC GGCGGTGATA TAGACGTTGT
  961 GGGCTGATGT AGTTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGGTCC TTCCTTTGAA
 1021 AGTCGATGCC CTTTCAGCTC GATGCGGGTT CAACCAGGGT GTTCGTCCTT CGAACTTTCA
 1081 ACCTTCGGGC GCGGGTCTTT GTAGTTGGCC GTCGATCTTG AAGAGGATGG TTGGCGCTCT
1141 GACGTAAGCC CTTCGGGCAT GAACGGAACT TTTGGAAAGG A
//
Primer: EF1a-N_R2 RDB15253_A7H9d.seq
>D04498G2-1_A7H9_EF1a-N_R2_A11_02_ABI24.ab1
    1 GTGGCGGGGC AAGATTGCAG TTTGATTTGC ATTGGAAGGG TCGCTGGCTT TTGTGTTACA
   61 CGCCCCTTAT TTGTGCTTGA TTAGATGATC CCCCTGCTCG AGGGCCCATC TGGCCTGTGT
  121 TTCAGACACC AGGGAGTCTC TGCTCACGTT TCCTGCTATT TGCAGCCTCT CTATCAAGAC
  181 TAATACACCT CTTCCCGCAT CGGCTGCCTG TGAGAGGCTT TTCAGCACTG CAGGATTGCT
  241 TTTCAGCCCC AAAAGAGCTA GGCTTGACAC TAACAATTTT GAGAATCAGC TTCTACTGAA
  301 GTTAAATCTG AGGTTTTACA ACTTTGAGTA GCGTGTACTG GCATTAGATT GTCTGTCTTA
  361 TAGTTTGATA ATTAAATACA AACAGTTCTA AAGCAGGATA AAACCTTGTA TGCATTTCAT
  421 TTAATGTTTT TTGAGATTAA AAGCTTAAAC AAGAATCTCT AGTTTTCTTT CTTGCTTTTA
  481 CTTTTACTTC CTTAATACTC AAGTACAATT TTAATGGAGT ACTTTTTTAC TTTTACTCAA
  541 GTAAGATTCT AGCCAGATAC TTTTACTTTT AATTGAGTAA AATTTTCCCT AAGTACTTGT
  601 ACTTTCACTT GAGTAAAATT TTTGAGTACT TTTTACACCT CTGTCAAGAA CTCCTGGACA
  661 AACCTCTGAC CTGTGTGGAA CAGAGTGGAT ATGGGTGTCT GAACAGATAT TCACGTCTTT
  721 TGCAGATCAG AGGGCATTTC TGGTGTTGTG CAATGACCTG GGTCCAACAG GTAGGCGTAC
  781 TCGTATCCAA GAGCATTGTT GGACAGGAGG TAGTCTCCAT TCCTATAAAG AGGAAGGAAT
  841 GGCACCATGT AGTAGCCGTC ATTGTGGCCA ATTGGTGCAT TGGTACGTGG GTAGATGGAC
  901 CGGGGAGGCT GATGAGTTCT TAGCCAGCGC TCAAAGATGC TAGAAATGAT GTAAGAAACA
  961 AATATGCAGA TGAAAGTTTA AGATAAGATA AAAAAATTTA AACAATAATG CATTTATGGT
 1021 TTTGTGTTTC TATTCCACCA ATAATAACCT CACGCAGTAA AGCCACCAGA TATTTTTGGT
1081 TCGTTGGCTC TGCCAGGCAC GAAACCTCTC TTAGC
//
Primer: pT2KXIG#1 RDB15253_A7H9e.seq
>D04498G2_1_A7H9_pT2KXIG#1_E01_05_ABI08.ab1
    1 ACGCGCAAAT AACCCTCACT AAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC
   61 GCTCTAGAAC TAGTGGATCT GCTGGGCTTG CTGAAGGTAG GGGGTCAAGA ACCAGAGGTG
  121 TAAAGTACTT GAGTAATTTT ACTTGATTAC TGTACTTAAG TATTATTTTT GGGGATTTTT
  181 ACTTTACTTG AGTACAATTA AAAATCAATA CTTTTACTTT TACTTAATTA CATTTTTTTA
  241 GAAAAAAAAG TACTTTTTAC TCCTTACAAT TTTATTTACA GTCAAAAAGT ACTTATTTTT
  301 TGGAGATCAC TTCATTCTAT TTTCCCTTGC TATTACCAAA CCAATTGAAT TGCGCTGATG
  361 CCCAGTTTAA TTTAAATGTT ATTTATTCTG CCTATGAAAA TCGTTTTCAC ATTATATGAA
  421 ATTGGTCAGA CATGTTCATT GGTCCTTTGG AAGTGACGTC ATGTCACATC TATTACCACA
  481 ATGCACAGCA CCTTGACCTG GAAATTAGGG AAATTATAAC AGTCAATCAG TGGAAGAAAA
  541 TGGAGGAAGT ATGTGATTCA TCAGCAGCTG CGAGCAGCAC AGTCCAAAAT CAGCCACAGG
  601 ATCAAGAGCA CCCGTGGCCG TATCTTCGCA GATCTGATCT AGAGGATCAT AATCAGCCAT
  661 ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG
  721 AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC
  781 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT
  841 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCATCAT CGATGAATTA
  901 ATTCGAGCTC CACCGCGGTG GCGGCCGCCA GTGTGATGGA TATCTGCAGA ATTCTTAGTC
  961 CTCGATGTTG TGGCGGATCT TGAAGTTGGC CTTTGATGCC CGTTCTTCTG CTTGTCGGCG
 1021 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCCAG CTGTGCCCCA GGATGTTGCC
 1081 GTCCTCCTTG GAAGTCGATG CCTTCAGCTC GGATGCCGAT CACAGTGTCG CCTCGAACTT
1141 CACTTCGACG CGGGGTCTTT GGTAGAATGC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268.
reference Schilling, T.F., Visualizing retinoic acid morphogen gradients. Methods Cell Biol. 133: 139-163 (2016). PMID 27263412.

2019.02.26

GNP_filter3_RDBDEP_html_190131.pl