Resource data sheet
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GEPRA-G/ pT2KXIGdeltain (#RDB15253)

Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Shimozono, S., Nature 496 (7445): 363-366 (2013). PMID 23563268. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-gamma. For generation of transgenic zebrafish.
Vector backbone pT2KXIG deltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC
Gene/insert name mouse RARgamma cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S. et al., Nature 496 (7445): 363-366 (2013).
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S. et al., Nature 496 (7445): 363-366 (2013).
付加的使用条件：
Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee
RDB15253 GEPRA-G/ pT2KXIGdeltain DNA solution

How to cite this biological resource

Materials & Methods section:

The GEPRA-G/ pT2KXIGdeltain was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15253).

Reference section:

Shimozono, S., Iimura, T., Kitaguchi, T., Higashijima, S., Miyawaki, A., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. [PubMed] [Article] [RRC of NBRP]
review Schilling, T.F., Visualizing retinoic acid morphogen gradients. Methods Cell Biol. 133: 139-163 (2016). PMID 27263412. [PubMed] [Article]