RDB15253 - GEPRA-G/ pT2KXIGdeltain

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

GEPRA-G/ pT2KXIGdeltain (#RDB15253)

Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish.

Clone info.	Expression vector of genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-gamma. For generation of transgenic zebrafish.
Vector backbone	pT2KXIG deltain (plasmid)
Size of vector backbone	8.1 kb
Selectable markers	Amp^r
Growth conditions	LB + Amp, 37oC
Gene/insert name	mouse RARgamma cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S. et al., Nature 496 (7445): 363-366 (2013).
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Shimozono, S. et al., Nature 496 (7445): 363-366 (2013). Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Shimozono, S. et al., Nature 496 (7445): 363-366 (2013). 付加的使用条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
備考	提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15253	GEPRA-G/ pT2KXIGdeltain	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The GEPRA-G/ pT2KXIGdeltain was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15253).

Reference section:

Shimozono, S., Iimura, T., Kitaguchi, T., Higashijima, S., Miyawaki, A., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15253_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F2 Sequence file: RDB15253_A7H9a.seq
>D04498G2-1_A7H9_EF1a-C_F2_G10_19_ABI24.ab1 1 GTTGGGGTGT AAGGCCCGGT TCGCTCTCTT CCTCACCGGG TCTGCGGCGA GTTCTAGCTG 61 AAGCTTCCTG CAGGTCGACC GATCCTGAGA ACTTCAGGGT GAGTTTGGGG ACCCTTGATT 121 GTTCTTTCTT TTTCGCTATT GTAAAATTCA TGTTATATGG AGGGGGCAAA GTTTTCAGGG 181 TGTTGTTTAG AATGGGAAGA TGTCCCTTGT ATCACCATGG ACCCTCATGA TAATTTTGTT 241 TCTTTCACTT TCTACTCTGT TGACAACCAT TGTCTCCTCT TATTTTCTTT TCATTTTCTG 301 TAACTTTTTC GTTAAACTTT AGCTTGCATT TGTAACGAAT TTTTAAATTC ACTTTTGTTT 361 ATTTGTCAGA TTGTAAGTAC TTTCTCTAAT CACTTTTTTT TCAAGGCAAT CAGGGTATAT 421 TATATTGTAC TTCAGCACAG TTTTAGAGAA CAATTGTTAT AATTAAATGA TAAGGTAGAA 481 TATTTCTGCA TATAAATTCT GGCTGGCGTG GAAATATTCT TATTGGTAGA AACAACTACA 541 CCCTGGTCAT CATCCTGCCT TTCTCTTTAT GGTTACAATG ATATACACTG TTTGAGATGA 601 GGATAAAATA CTCTGAGTCC AAACCGGGCC CCTCTGCTAA CCATGTTCAT GCCTTCTTCT 661 CTTTCCTACA GCTCCTGGGC AACGTGCTGG TTGTTGTGCT GTCTCATCAT TTTGGCAAAG 721 AATTCCTCGA CGGATCCCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC 781 ATCCTGGTCG AGCTGCAGGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC 841 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG 901 CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGAGCTGGG GCGTGCAGTG CTTCAGCCGC 961 TACCCCGACC ACATGAAAGC AGCACGACTT TCTTCAAGTC CGTCATGCCC GAATGCTACG 1021 TCCAGGAGCG CACCAATCTT CTTCAGGACG ACGGCACTAC AAGACCGGCG CGAGTGAGGT 1081 TCGAAGGCGA CACCTGGTGG ATCGCAATCG AGCTGGAAAG GGCCATCCGA ACCTT //
Primer: pAxCALNL_F1 Sequence file: RDB15253_A7H9b.seq
>D04498G2_1_A7H9_pAxCALNLF1_D01_04_ABI08.ab1 1 GAAAGGGTAT CTTATCATGT CTGGATCATC ATCGATGAAT TAATTCGAGC TCCACCGCGG 61 TGGCGGCCGC CAGTGTGATG GATATCTGCA GAATTCTTAG TCCTCGATGT TGTGGCGGAT 121 CTTGAAGTTG GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT 181 GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC 241 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT 301 GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC 361 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC 421 GTAGCCCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAG 481 CAGCTTCAGG GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA 541 CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG 601 CTCCTCGCCC TTGCTCACCA TGCCACCGGT ACCGCCCTTG TACAGCTCGT TCATGCCTTC 661 AGTGATCCCG GCGGCGGTCA GGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG 721 GTCTTTGAAC AGGGCGGACT GGTAGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC 781 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCCGC CCATGAGCTC 841 CTCAAACATC TCCGGGTTCT CCAGCATCTC TCGGATCAGG GGTGGCATCG GGCCTGGAAT 901 CTCCATCTTC AGGGTTATAG CCCTTTCTGC TCCCTTAGTG CTGATGCCCC GGAGGTCGGG 961 TGATTTTCAT CAGCATCCTT GGGAACATGT AGGGTTGGCT GGGTCTCCGG TCGCCGGGCA 1021 ATAGAGCCCT CAGGCTTTCC AAGCAGGGGC CTCCTGCAGC TTGTCATCTT CTCGGGCTTC 1081 TTCAGGTCCA TCGGTCTTCC ACAGAATTGA GCCAGATAGC ACTTAGTTAG CTCCAGTCTC 1141 GTGATCATTC CATTTCTTCC AAGCG //
Primer: RARgamma_LBD Sequence file: RDB15253_A7H9c.seq
>D04498G2_1_A7H9_RARgamma_LBD_F01_06_ABI08.ab1 1 CTGAATAGGG GGTGGCATCG GGGGCCTGGG AATCTCCATC TTCAGGGTTA TAGCCCTTTC 61 TGCTCCCTTA GTGCTGATGC CCCGGAGGTC GGTGATTTTC ATCAGCATCC TTGGGAACAT 121 GTAGGGTTGG CTGGGTCTCC GTCGCCGGGC ATAGAGCCTC AGGGCTTCCA GCAGGGGCTC 181 CTGCAGCTTG TCCACCTTCT CGGGCTCTTC CAGGTCCATT CGGTCTCCAC AGATGAGGCA 241 GATAGCACTA AGTAGCCCAG TCTCGGTGTC ATCCATCTCC AGGGGCAGCA GCTGCCCGGC 301 AAAGGCAAAG ACGAGGTCTG TAAGGGGCCC AAAGCCAGCA TTGTGCATCT GGGTTCGGTT 361 CAGGGTCAGC CCATCCGAGA ATGTCATAGT GTCCTGCTCT GGGGTATACC TTGTACAGAT 421 CCGCAGCATT AGGATGTCCA GACAAGCAGC CTTGAGCAGC GTGATCTGGT CGGCAATGCT 481 GAGCCCTGTA AAACCAGGCA GCCGCTTCGC AAACTCCACA ATCTTGATGA TGCATTTGGT 541 GGCCAGCTCG CTGAACTTGT CCCACAGCCC CAGGTCCAGC TGCACCCGGT GATCTGCACT 601 GGAGTTCGTG GTGTACTTGC CCAGCTGGCA GAGTGAGGGA AAAGTCTCCT GGTGGGCTTT 661 GCTGACCTTG GTGATGAGTT CCTCTAACTG TGGACTCAGT TCATAGCTGT CATGCATGCG 721 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT 781 CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGG TTCACGGGGC CGTCGCCGAT 841 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCACGA TGTTGTGGCG 901 GATCTTGAAG TTTGGCCTTG ATGCCGTTCT TCTGCTTGTC GGCGGTGATA TAGACGTTGT 961 GGGCTGATGT AGTTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGGTCC TTCCTTTGAA 1021 AGTCGATGCC CTTTCAGCTC GATGCGGGTT CAACCAGGGT GTTCGTCCTT CGAACTTTCA 1081 ACCTTCGGGC GCGGGTCTTT GTAGTTGGCC GTCGATCTTG AAGAGGATGG TTGGCGCTCT 1141 GACGTAAGCC CTTCGGGCAT GAACGGAACT TTTGGAAAGG A //
Primer: EF1a-N_R2 Sequence file: RDB15253_A7H9d.seq
>D04498G2-1_A7H9_EF1a-N_R2_A11_02_ABI24.ab1 1 GTGGCGGGGC AAGATTGCAG TTTGATTTGC ATTGGAAGGG TCGCTGGCTT TTGTGTTACA 61 CGCCCCTTAT TTGTGCTTGA TTAGATGATC CCCCTGCTCG AGGGCCCATC TGGCCTGTGT 121 TTCAGACACC AGGGAGTCTC TGCTCACGTT TCCTGCTATT TGCAGCCTCT CTATCAAGAC 181 TAATACACCT CTTCCCGCAT CGGCTGCCTG TGAGAGGCTT TTCAGCACTG CAGGATTGCT 241 TTTCAGCCCC AAAAGAGCTA GGCTTGACAC TAACAATTTT GAGAATCAGC TTCTACTGAA 301 GTTAAATCTG AGGTTTTACA ACTTTGAGTA GCGTGTACTG GCATTAGATT GTCTGTCTTA 361 TAGTTTGATA ATTAAATACA AACAGTTCTA AAGCAGGATA AAACCTTGTA TGCATTTCAT 421 TTAATGTTTT TTGAGATTAA AAGCTTAAAC AAGAATCTCT AGTTTTCTTT CTTGCTTTTA 481 CTTTTACTTC CTTAATACTC AAGTACAATT TTAATGGAGT ACTTTTTTAC TTTTACTCAA 541 GTAAGATTCT AGCCAGATAC TTTTACTTTT AATTGAGTAA AATTTTCCCT AAGTACTTGT 601 ACTTTCACTT GAGTAAAATT TTTGAGTACT TTTTACACCT CTGTCAAGAA CTCCTGGACA 661 AACCTCTGAC CTGTGTGGAA CAGAGTGGAT ATGGGTGTCT GAACAGATAT TCACGTCTTT 721 TGCAGATCAG AGGGCATTTC TGGTGTTGTG CAATGACCTG GGTCCAACAG GTAGGCGTAC 781 TCGTATCCAA GAGCATTGTT GGACAGGAGG TAGTCTCCAT TCCTATAAAG AGGAAGGAAT 841 GGCACCATGT AGTAGCCGTC ATTGTGGCCA ATTGGTGCAT TGGTACGTGG GTAGATGGAC 901 CGGGGAGGCT GATGAGTTCT TAGCCAGCGC TCAAAGATGC TAGAAATGAT GTAAGAAACA 961 AATATGCAGA TGAAAGTTTA AGATAAGATA AAAAAATTTA AACAATAATG CATTTATGGT 1021 TTTGTGTTTC TATTCCACCA ATAATAACCT CACGCAGTAA AGCCACCAGA TATTTTTGGT 1081 TCGTTGGCTC TGCCAGGCAC GAAACCTCTC TTAGC //
Primer: pT2KXIG#1 Sequence file: RDB15253_A7H9e.seq
>D04498G2_1_A7H9_pT2KXIG#1_E01_05_ABI08.ab1 1 ACGCGCAAAT AACCCTCACT AAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC 61 GCTCTAGAAC TAGTGGATCT GCTGGGCTTG CTGAAGGTAG GGGGTCAAGA ACCAGAGGTG 121 TAAAGTACTT GAGTAATTTT ACTTGATTAC TGTACTTAAG TATTATTTTT GGGGATTTTT 181 ACTTTACTTG AGTACAATTA AAAATCAATA CTTTTACTTT TACTTAATTA CATTTTTTTA 241 GAAAAAAAAG TACTTTTTAC TCCTTACAAT TTTATTTACA GTCAAAAAGT ACTTATTTTT 301 TGGAGATCAC TTCATTCTAT TTTCCCTTGC TATTACCAAA CCAATTGAAT TGCGCTGATG 361 CCCAGTTTAA TTTAAATGTT ATTTATTCTG CCTATGAAAA TCGTTTTCAC ATTATATGAA 421 ATTGGTCAGA CATGTTCATT GGTCCTTTGG AAGTGACGTC ATGTCACATC TATTACCACA 481 ATGCACAGCA CCTTGACCTG GAAATTAGGG AAATTATAAC AGTCAATCAG TGGAAGAAAA 541 TGGAGGAAGT ATGTGATTCA TCAGCAGCTG CGAGCAGCAC AGTCCAAAAT CAGCCACAGG 601 ATCAAGAGCA CCCGTGGCCG TATCTTCGCA GATCTGATCT AGAGGATCAT AATCAGCCAT 661 ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG 721 AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC 781 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT 841 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCATCAT CGATGAATTA 901 ATTCGAGCTC CACCGCGGTG GCGGCCGCCA GTGTGATGGA TATCTGCAGA ATTCTTAGTC 961 CTCGATGTTG TGGCGGATCT TGAAGTTGGC CTTTGATGCC CGTTCTTCTG CTTGTCGGCG 1021 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCCAG CTGTGCCCCA GGATGTTGCC 1081 GTCCTCCTTG GAAGTCGATG CCTTCAGCTC GGATGCCGAT CACAGTGTCG CCTCGAACTT 1141 CACTTCGACG CGGGGTCTTT GGTAGAATGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. [link to RRC of NBRP]
review	Schilling, T.F., Visualizing retinoic acid morphogen gradients. Methods Cell Biol. 133: 139-163 (2016). PMID 27263412.