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h2-3/pRSETb (#RDB18219)

New coral derived green-emitting fluorescent protein h2-3.

Clone info. New coral derived green-emitting fluorescent protein h2-3.
Comment The accession numbers in the DDBJ/EMBL/GenBank database is LC085680.
Vector backbone pRSETb (plasmid)
Size of vector backbone 3.6 kb
Selectable markers Ampicillin
Growth conditions LB+Amp, 37oC
Gene/insert name Ricordea sp. GFP cDNA
Reference of insert sequence LC085680 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Sawano, Asako | Ando, Ryoko |
 
Sequence (full) RDB18219hts01.seq checkAssembled from experimentally sequenced data.
Publication Ando, R., Cell Struct. Funct. 48 (2): 135-144 (2023). PMID 37394513. [link to RRC of NBRP]
Test sheet RDB18219_B0Gfp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Ando, R. et al., bioRxiv doi: 10.1101/2020.03.30.015156 (2020).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Ando, R. et al., bioRxiv doi: 10.1101/2020.03.30.015156 (2020).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18219 h2-3/pRSETb DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The h2-3/pRSETb was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18219).

Reference section:

Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A., Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. Cell Struct. Funct. 48 (2): 135-144 (2023). PMID 37394513. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ando, R., Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. Cell Struct. Funct. 48 (2): 135-144 (2023). PMID 37394513. [link to RRC of NBRP]