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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

h2-3/pRSETb

New coral derived green-emitting fluorescent protein h2-3.

Catalog number RDB18219
Resource name h2-3/pRSETb
Clone info. New coral derived green-emitting fluorescent protein h2-3.
The accession numbers in the DDBJ/EMBL/GenBank database is LC085680.
Vector backbone pRSETb (plasmid)
Size of vector backbone 3.6 kb
Selectable markers Ampicillin
Gene/insert name Ricordea sp. GFP cDNA
Reference of nucleotide sequence LC085680 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Sakaue-Sawano, Asako | Ando, Ryoko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18219 h2-3/pRSETb DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18219_B0Fbp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: T7pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB18219_B0Fba.seq check
>D07168A2_B0Fb_2_lacIpro-B_F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNCGCNN CGNNTCNGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC
   61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT
  121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC
  181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA
  241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGG
  301 CCACCATGAG TAAACTCCAG AAAGGTGTGG AGAAAGAGAT GAAGATTAAG CTCACTATGA
  361 ACTGCACCGT GAACGAACGG AACTTTGTGA TCACAGGGCA GGGGGCTGGG GAACCTTATG
  421 ATGGAACTCA GACACTTTAC CTGACAGTGG AGGGCGGAAA GACACTGAAC TTCAGCTTCG
  481 ATGTACTGAC CCCAGCCTTT CAGTACGGCA ATAGGGCGTT TACCAAGTAC CCCGGTAACA
  541 TACCAGACTT CTTCAAACAG ACCTTCAGTG GCGGGGGATA CACATGGAAA AGAACAATGA
  601 CCTATGAAGA CGGCGGCGTC TCAACCGTTG AGTCCGATAT CAGCGTTCAA GGGGACTGCT
  661 TCCATTATAA AATACAATTC AACGGAAAGT TCCCCCCCCA CGGCCCAGTC ATGCAAAAGG
  721 AAACAGTCAA ATGGGAACCT TCCACCGANG TGATGTACNA GGACGATNAA AACGATGGAN
  781 TGCTGAANGG CGANGTGAAT ATGGNCCTGC TGNTGAAGGA NGGTGGTCAC CTCAGANNCN
  841 ANTTCNACAC TTCTTATANN NCCNAAAATN ANNNNGANAA GANGNNAGAN TANCACTTCN
  901 NGGNNCNCCN GNNTNNNNNT NCNGGNNAAN CCCNNNNNNN NCNNNNNNNG NNNNACNNNN
961 GGNNNNNNNN NN
//
Primer: T7ter_R1
Region: insert 3'
Sequence file: RDB18219_B0Fbb.seq check
>D07168A2_B0Fb_2_T7ter_R1_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTN NNNNNNNNNN NNNGNNNNNN GGTGGCNNGC AGCCAACTCA
   61 GCTTCCTTTC GGGCTTTGTT AGCAGCCGGA TCAAGCTTCG AATTCTCACT TATTCAGGTT
  121 CTTTTCAGGC AGCAGGCTGT ACCTGGCCAC AGCCCCTGCG TAGAGCTTGA CAGGGCGTCC
  181 TTCGGGCTTC TCCAGGATCT CGATCCGGTG GTCCACGAAG TGGTAATCTG GCATCTTCTC
  241 CACCTTATTT TTGGGAATAT AAGAAGTGTT GAAATCGACT CTGAGGTGAC CACCATCCTT
  301 CAACAGCAGG GCCATATTCA CGTCGCCCTT CAGCACTCCA TCGTTTTTAT CGTCCTTGTA
  361 CATCACCTCG GTGGAAGGTT CCCATTTGAC TGTTTCCTTT TGCATGACTG GGCCGTGGGG
  421 GGGGAACTTT CCGTTGAATT GTATTTTATA ATGGAAGCAG TCCCCTTGAA CGCTGATATC
  481 GGACTCAACG GTTGAGACGC CGCCGTCTTC ATAGGTCATT GTTCTTTTCC ATGTGTATCC
  541 CCCGCCACTG AAGGTCTGTT TGAAGAAGTC TGGTATGTTA CCGGGGTACT TGGTAAACGC
  601 CCTATTGCCG TACTGAAAGG CTGGGGTCAG TACATCGAAG CTGAAGTTCA GTGTCTTTCC
  661 GCCCTCCACT GTCAGGTAAA GTGTCTGAGT TCCATCATAA GGTTCCCCAG CCCCCTGCCC
  721 TGTGATCACA AAGTTCCGTT CGTNCNNNGT GCAGTTCATA GTGAGCTNNA NTCTTCATCT
  781 CTTTCTCCAC NCCTTTCTGG ANTTTACTCA NGGTGGCCGG ATCCTTATCG TCATCGTCGT
  841 ACAGATCCCG ACCCATTTGC TGNCCANNAG TCATGCTAGC CATACCATGA TNNNNNATGA
  901 NGATGAGANN CCCGCATATG TATATCTCCT TNTNNAAGTT AANNAAATTA TTTCTANNNG
  961 GNAAANCNNT TNNGGNNNNC CTNNNNNGAN NCNNNNTAAT TTNCGNNGGG ANCGANANNC
1021 NNNCANTNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

2020.12.17

GNP_filter3_RDBDEP_html_201216.pl