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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

h2-3/pRSETb

New coral derived green-emitting fluorescent protein h2-3.

Catalog number RDB18219
Resource name h2-3/pRSETb
Clone info. New coral derived green-emitting fluorescent protein h2-3.
Comment The accession numbers in the DDBJ/EMBL/GenBank database is LC085680.
Vector backbone pRSETb (plasmid)
Size of vector backbone 3.6 kb
Selectable markers Ampicillin
Gene/insert name Ricordea sp. GFP cDNA
Reference of insert sequence LC085680 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Sakaue-Sawano, Asako | Ando, Ryoko |
Other clones in our bank
External Database
Ricordea sp. GFP
            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
            4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form             [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
            4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18219 h2-3/pRSETb DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18219_B0Fbp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: T7pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
            Sequence file: RDB18219_B0Fba.seq check
            >D07168A2_B0Fb_2_lacIpro-B_F_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNCGCNN CGNNTCNGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC
               61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT
              121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC
              181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA
              241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGG
              301 CCACCATGAG TAAACTCCAG AAAGGTGTGG AGAAAGAGAT GAAGATTAAG CTCACTATGA
              361 ACTGCACCGT GAACGAACGG AACTTTGTGA TCACAGGGCA GGGGGCTGGG GAACCTTATG
              421 ATGGAACTCA GACACTTTAC CTGACAGTGG AGGGCGGAAA GACACTGAAC TTCAGCTTCG
              481 ATGTACTGAC CCCAGCCTTT CAGTACGGCA ATAGGGCGTT TACCAAGTAC CCCGGTAACA
              541 TACCAGACTT CTTCAAACAG ACCTTCAGTG GCGGGGGATA CACATGGAAA AGAACAATGA
              601 CCTATGAAGA CGGCGGCGTC TCAACCGTTG AGTCCGATAT CAGCGTTCAA GGGGACTGCT
              661 TCCATTATAA AATACAATTC AACGGAAAGT TCCCCCCCCA CGGCCCAGTC ATGCAAAAGG
              721 AAACAGTCAA ATGGGAACCT TCCACCGANG TGATGTACNA GGACGATNAA AACGATGGAN
              781 TGCTGAANGG CGANGTGAAT ATGGNCCTGC TGNTGAAGGA NGGTGGTCAC CTCAGANNCN
              841 ANTTCNACAC TTCTTATANN NCCNAAAATN ANNNNGANAA GANGNNAGAN TANCACTTCN
              901 NGGNNCNCCN GNNTNNNNNT NCNGGNNAAN CCCNNNNNNN NCNNNNNNNG NNNNACNNNN
            961 GGNNNNNNNN NN
            //
            Primer: T7ter_R1
            Region: insert 3'
            Sequence file: RDB18219_B0Fbb.seq check
            >D07168A2_B0Fb_2_T7ter_R1_C02_08_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNTN NNNNNNNNNN NNNGNNNNNN GGTGGCNNGC AGCCAACTCA
               61 GCTTCCTTTC GGGCTTTGTT AGCAGCCGGA TCAAGCTTCG AATTCTCACT TATTCAGGTT
              121 CTTTTCAGGC AGCAGGCTGT ACCTGGCCAC AGCCCCTGCG TAGAGCTTGA CAGGGCGTCC
              181 TTCGGGCTTC TCCAGGATCT CGATCCGGTG GTCCACGAAG TGGTAATCTG GCATCTTCTC
              241 CACCTTATTT TTGGGAATAT AAGAAGTGTT GAAATCGACT CTGAGGTGAC CACCATCCTT
              301 CAACAGCAGG GCCATATTCA CGTCGCCCTT CAGCACTCCA TCGTTTTTAT CGTCCTTGTA
              361 CATCACCTCG GTGGAAGGTT CCCATTTGAC TGTTTCCTTT TGCATGACTG GGCCGTGGGG
              421 GGGGAACTTT CCGTTGAATT GTATTTTATA ATGGAAGCAG TCCCCTTGAA CGCTGATATC
              481 GGACTCAACG GTTGAGACGC CGCCGTCTTC ATAGGTCATT GTTCTTTTCC ATGTGTATCC
              541 CCCGCCACTG AAGGTCTGTT TGAAGAAGTC TGGTATGTTA CCGGGGTACT TGGTAAACGC
              601 CCTATTGCCG TACTGAAAGG CTGGGGTCAG TACATCGAAG CTGAAGTTCA GTGTCTTTCC
              661 GCCCTCCACT GTCAGGTAAA GTGTCTGAGT TCCATCATAA GGTTCCCCAG CCCCCTGCCC
              721 TGTGATCACA AAGTTCCGTT CGTNCNNNGT GCAGTTCATA GTGAGCTNNA NTCTTCATCT
              781 CTTTCTCCAC NCCTTTCTGG ANTTTACTCA NGGTGGCCGG ATCCTTATCG TCATCGTCGT
              841 ACAGATCCCG ACCCATTTGC TGNCCANNAG TCATGCTAGC CATACCATGA TNNNNNATGA
              901 NGATGAGANN CCCGCATATG TATATCTCCT TNTNNAAGTT AANNAAATTA TTTCTANNNG
              961 GNAAANCNNT TNNGGNNNNC CTNNNNNGAN NCNNNNTAAT TTNCGNNGGG ANCGANANNC
            1021 NNNCANTNNN NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

            2022.05.16

            GNP_filter3_RDBDEP_html_220516.pl