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pET28/Dronpa-NT-Lys (#RDB15470)

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.


Drawn by SnapGene® software
Sequence (full) RDB15470hts02.seq
Publication Mizuno, H., Chem. Sci. 2: 1548-1553 (2011). [link to RRC of NBRP]
Test sheet RDB15470_B4Eup1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
Vector backbone pET-28 (Plasmid)
Gene/insert name Lysenin
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Mizuno, H. et al., Chem. Sci. 2: 1548-1553 (2011).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Mizuno, H. et al., Chem. Sci. 2: 1548-1553 (2011).
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15470 pET28/Dronpa-NT-Lys DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pET28/Dronpa-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15470).

Reference section:

Mizuno, H., Abe, M., Dedecker, P., ARocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011). [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Mizuno, H., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011). [link to RRC of NBRP]