RDB15470 - pET28/Dronpa-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.

Distribution information

Catalog number	RDB15470
Resource name	pET28/Dronpa-NT-Lys
Clone info.	Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
Vector backbone	Plasmid
Gene/insert name	Lysenin
Depositor\|Developer	Miyawaki, Atsushi \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Mizuno, H., Abe, M., Dedecker, P., Makino, A., Rocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A. (2011) Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Mizuno, H., Abe, M., Dedecker, P., Makino, A., Rocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A. (2011) Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15470	pET28/Dronpa-NT-Lys	DNA solution

original	Mizuno, H., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011).

Featured content	Visualization of Organelles (English text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: pBR_BamHI_R2 Sequence file: RDB15470_A7Jqa.seq
>D04498J7_1_A7Jq_pBR_BamHI_R2_A03_03_ABI24.ab1 1 CTGCTGGAGT TTACCAGCGG GAGCGGCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG 61 ATCCCGCGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC AATTCCCCTC 121 TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGGCAGCAG CCATCATCAT 181 CATCATCACA GCAGCGGCCT GGTGCCGCGC GGCAGCGGTA CCATGAGTGT GATTAAACCA 241 GACATGAAGA TCAAGCTGCG TATGGAAGGC GCTGTAAATG GACACCCGTT CGCGATTGAA 301 GGAGTTGGCC TTGGGAAGCC TTTCGAGGGA AAACAGAGTA TGGACCTTAA AGTCAAAGAA 361 GGCGGACCTC TGCCTTTCGC CTATGACATC TTGACAACTG TGTTCTGTTA CGGCAACAGG 421 GTATTCGCCA AATACCCAGA AAATATAGTA GACTATTTCA AGCAGTCGTT TCCTGAGGGC 481 TACTCTTGGG AACGAAGCAT GAATTACGAA GACGGGGGCA TTTGTAACGC GACAAACGAC 541 ATAACCCTGG ATGGTGACTG TTATATCTAT GAAATTCGAT TTGATGGTGT GAACTTTCCT 601 GCCAATGGTC CAGTTATGCA GAAGAGGACT GTGAAATGGG AGCCATCCAC TGAGAAATTG 661 TATGTGCGTG ATGGAGTGCT GAAGGGTGAT GTTAACATGG CTCTGTCGCT TGAAGGAGGT 721 GGCCATTACC GATGTGACTT CAAAACTACT TATAAAGCTA AGAAGGTTGT CCAGTTGCCA 781 GACTATCACT TTGTGGACCA CCACATTGAG ATTAAAAGCC ACGACAAAGA TTACAGTAAT 841 GTTAATCTGC ATGAGCATGC CGAAGCGCAT TCTGAGCTGC CGAGGCAGGC CAAGAAGCTT 901 ATAATCCTTG GAAAAACAGA AAATTCGAAT TAAGCATGCA GAAAAGGAAG GAGTACATGA 961 CAGTCGTTTC AAGAAAAAGG TGGCCAGCTG CAAACTCTTG GACATAGCAA AACTTTTCCA 1021 AGTTTGTTGC TCTAATGAAA GAATGGGGGG GATTTTCGGA AGTTAAAAAA CCCGCTGTGG 1081 A //
Primer: T7 terminator Sequence file: RDB15470_A7Jqb.seq
>D04498J7_A7Jq_1_1_T7 terminator_F04_16_ABI24.ab1 1 GATCAGCCTT CTTTCGGGCT TTGTTAGCAG CCGGATCTCA GTGGTGGTGG TGGTGGTGCT 61 CGAGTTAACC AACCACTTCC AAAATCCACT TGTCTTCACG TTTGTCCAGA CAGTACACGT 121 TTGTTGCCGG TCCATCATCG TAGCACAGCC CACTGCGAGT GAAGTACTTA TTCATGAAGG 181 TGACTACGTC ACCATGACGA AGAGGCAATG ACTTATTGAT TGCCCAGCGC TGTTTCGGAT 241 TATCAGTACC CTGATCAAAG TAGATTCCTC CTTGATCAGA GGAATAGGCA TACTCATAGC 301 CCGAATACAT GGTGTTCAGC GTTTTAATTC GAAATCCCCC CCAATCTTCA TAGAGCACAA 361 ACTTGAAAAG TTTGCTATGT CCAAGAGTTG CAGCTGGCCA ACTTTTTCTT GAAACGACTG 421 TCATGTACTC CTTCCTTTCT GCATGCTTAA TTCGAATTTC TGTTTTTCCA AGGATTATAA 481 GCTTCTTGGC CTGCCTCGGC AGCTCAGAAT GCGCTTCGGC ATGCTCATGC AGATTAACAT 541 TACTGTAATC TTTGTCGTGG CTTTTAATCT CAATGTGGTG GTCCACAAAG TGATAGTCTG 601 GCAACTGGAC AACCTTCTTA GCTTTATAAG TAGTTTTGAA GTCACATCGG TAATGGCCAC 661 CTCCTTCAAG CGACAGAGCC ATGTTAACAT CACCCTTCAG CACTCCATCA CGCACATACA 721 ATTTCTCAGT GGATGGCTCC CATTTCACAG TCCTCTTCTG CATAACTGGA CCATTGGCAG 781 GAAAGTTCAC ACCATCAAAT CGAATTTCAT AGATATAACA GTCACCATCC AGGGTTATGT 841 CGTTTGTCGC GTTACAAATG CCCCCGTCTT CGTAATTCAT GCTTCGTTCC CAAGAGTAGC 901 CCTCAGGAAA CGACTGCTTG AAATAGTCTA CTATATTTTC TGGGTATTTG GCGAATACCC 961 TGTTGCCGTA ACAGAACACA GTTGTCAAGA TGTCATAGCG AAAGGCAGAG GTCCGCCTTT 1021 CTTTGACTTT AAGTCCATAC TCTGGTTTTC CCTCGAAGGC TCCCAAGGCA ACTTCTCATC 1081 GCGAACGGAT GTCCATTTAC AGCGCCTCAT ACGCAGCCTG GAATCCTTCA TGTTCTGGAT 1141 TTATTCAACA ACTA //
Primer: RV4 Sequence file: RDB15470_A7Jqc.seq
>D04498J7-1_A7Jq_RV4_G10_19_ABI24.ab1 1 AAAGGCATGC TGGGTTGACG CTCTCAAGGG CATCGGTCGG AGATCCCGGT GCCTAATGAG 61 TGAGCTAACT TACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 121 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 181 GCCAGGGTGG TTTTTCTTTT CACCAGTGAG ACGGGCAACA GCTGATTGCC CTTCACCGCC 241 TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG GCGAAAATCC 301 TGTTTGATGG TGGTTAACGG CGGGATATAA CATGAGCTGT CTTCGGTATC GTCGTATCCC 361 ACTACCGAGA TATCCGCACC AACGCGCAGC CCGGACTCGG TAATGGCGCG CATTGCGCCC 421 AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA CGATGCCCTC ATTCAGCATT 481 TGCATGGTTT GTTGAAAACC GGACATGGCA CTCCAGTCGC CTTCCCGTTC CGCTATCGGC 541 TGAATTTGAT TGCGAGTGAG ATATTTATGC CAGCCAGCCA GACGCAGACG CGCCGAGACA 601 GAACTTAATG GGCCCGCTAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC CAGATGCTCC 661 ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC TGTTGATGGG TGTCTGGTCA 721 GAGACATCAA GAAATAACGC CGGAACATTA GTGCAGGCAG CTTCCACAGC AATGGCATCC 781 TGGTCATCCA GCGGATAGTT AATGATCAGC CCACTGACGC GTTGCGCGAG AAGATTGTGC 841 ACCGCCGCTT TACAGGCTTC GACGCCGCTT CGTTCTACCA TCGACACCAC CACGCTGGCA 901 CCCAGTTGAT CGGCGTGGAG ATTTAATCGC CGCGACAATT TGCGACGGCG CGTTGCAGGG 961 CCAGACTGGG ATGTGGCAAC GCCAATCAGC AACGACTGTT GCCCGCCCAG TTGTGTGCCA 1021 CGCGTTGGGG AATGTAAATC AGCCTCCGCC CATCGCGGCT TCAACTTTTT CCCGCGTTTT 1081 TCGCAGAATC GTGGCTTGCC CTGGTTCAAC ACGTCGGGGA //