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pET28/Dronpa-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.

Catalog number RDB15470
Resource name pET28/Dronpa-NT-Lys
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
Vector backbone Plasmid
Gene/insert name Lysenin
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Mizuno, H., Abe, M., Dedecker, P., Makino, A., Rocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A. (2011) Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15470 pET28/Dronpa-NT-Lys DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Mizuno, H., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011).

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Organelle marker (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15470_A7Jqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_R2 RDB15470_A7Jqa.seq
>D04498J7_1_A7Jq_pBR_BamHI_R2_A03_03_ABI24.ab1
    1 CTGCTGGAGT TTACCAGCGG GAGCGGCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG
   61 ATCCCGCGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC AATTCCCCTC
  121 TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGGCAGCAG CCATCATCAT
  181 CATCATCACA GCAGCGGCCT GGTGCCGCGC GGCAGCGGTA CCATGAGTGT GATTAAACCA
  241 GACATGAAGA TCAAGCTGCG TATGGAAGGC GCTGTAAATG GACACCCGTT CGCGATTGAA
  301 GGAGTTGGCC TTGGGAAGCC TTTCGAGGGA AAACAGAGTA TGGACCTTAA AGTCAAAGAA
  361 GGCGGACCTC TGCCTTTCGC CTATGACATC TTGACAACTG TGTTCTGTTA CGGCAACAGG
  421 GTATTCGCCA AATACCCAGA AAATATAGTA GACTATTTCA AGCAGTCGTT TCCTGAGGGC
  481 TACTCTTGGG AACGAAGCAT GAATTACGAA GACGGGGGCA TTTGTAACGC GACAAACGAC
  541 ATAACCCTGG ATGGTGACTG TTATATCTAT GAAATTCGAT TTGATGGTGT GAACTTTCCT
  601 GCCAATGGTC CAGTTATGCA GAAGAGGACT GTGAAATGGG AGCCATCCAC TGAGAAATTG
  661 TATGTGCGTG ATGGAGTGCT GAAGGGTGAT GTTAACATGG CTCTGTCGCT TGAAGGAGGT
  721 GGCCATTACC GATGTGACTT CAAAACTACT TATAAAGCTA AGAAGGTTGT CCAGTTGCCA
  781 GACTATCACT TTGTGGACCA CCACATTGAG ATTAAAAGCC ACGACAAAGA TTACAGTAAT
  841 GTTAATCTGC ATGAGCATGC CGAAGCGCAT TCTGAGCTGC CGAGGCAGGC CAAGAAGCTT
  901 ATAATCCTTG GAAAAACAGA AAATTCGAAT TAAGCATGCA GAAAAGGAAG GAGTACATGA
  961 CAGTCGTTTC AAGAAAAAGG TGGCCAGCTG CAAACTCTTG GACATAGCAA AACTTTTCCA
 1021 AGTTTGTTGC TCTAATGAAA GAATGGGGGG GATTTTCGGA AGTTAAAAAA CCCGCTGTGG
1081 A
//
Primer: T7 terminator RDB15470_A7Jqb.seq
>D04498J7_A7Jq_1_1_T7 terminator_F04_16_ABI24.ab1
    1 GATCAGCCTT CTTTCGGGCT TTGTTAGCAG CCGGATCTCA GTGGTGGTGG TGGTGGTGCT
   61 CGAGTTAACC AACCACTTCC AAAATCCACT TGTCTTCACG TTTGTCCAGA CAGTACACGT
  121 TTGTTGCCGG TCCATCATCG TAGCACAGCC CACTGCGAGT GAAGTACTTA TTCATGAAGG
  181 TGACTACGTC ACCATGACGA AGAGGCAATG ACTTATTGAT TGCCCAGCGC TGTTTCGGAT
  241 TATCAGTACC CTGATCAAAG TAGATTCCTC CTTGATCAGA GGAATAGGCA TACTCATAGC
  301 CCGAATACAT GGTGTTCAGC GTTTTAATTC GAAATCCCCC CCAATCTTCA TAGAGCACAA
  361 ACTTGAAAAG TTTGCTATGT CCAAGAGTTG CAGCTGGCCA ACTTTTTCTT GAAACGACTG
  421 TCATGTACTC CTTCCTTTCT GCATGCTTAA TTCGAATTTC TGTTTTTCCA AGGATTATAA
  481 GCTTCTTGGC CTGCCTCGGC AGCTCAGAAT GCGCTTCGGC ATGCTCATGC AGATTAACAT
  541 TACTGTAATC TTTGTCGTGG CTTTTAATCT CAATGTGGTG GTCCACAAAG TGATAGTCTG
  601 GCAACTGGAC AACCTTCTTA GCTTTATAAG TAGTTTTGAA GTCACATCGG TAATGGCCAC
  661 CTCCTTCAAG CGACAGAGCC ATGTTAACAT CACCCTTCAG CACTCCATCA CGCACATACA
  721 ATTTCTCAGT GGATGGCTCC CATTTCACAG TCCTCTTCTG CATAACTGGA CCATTGGCAG
  781 GAAAGTTCAC ACCATCAAAT CGAATTTCAT AGATATAACA GTCACCATCC AGGGTTATGT
  841 CGTTTGTCGC GTTACAAATG CCCCCGTCTT CGTAATTCAT GCTTCGTTCC CAAGAGTAGC
  901 CCTCAGGAAA CGACTGCTTG AAATAGTCTA CTATATTTTC TGGGTATTTG GCGAATACCC
  961 TGTTGCCGTA ACAGAACACA GTTGTCAAGA TGTCATAGCG AAAGGCAGAG GTCCGCCTTT
 1021 CTTTGACTTT AAGTCCATAC TCTGGTTTTC CCTCGAAGGC TCCCAAGGCA ACTTCTCATC
 1081 GCGAACGGAT GTCCATTTAC AGCGCCTCAT ACGCAGCCTG GAATCCTTCA TGTTCTGGAT
1141 TTATTCAACA ACTA
//
Primer: RV4 RDB15470_A7Jqc.seq
>D04498J7-1_A7Jq_RV4_G10_19_ABI24.ab1
    1 AAAGGCATGC TGGGTTGACG CTCTCAAGGG CATCGGTCGG AGATCCCGGT GCCTAATGAG
   61 TGAGCTAACT TACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT
  121 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
  181 GCCAGGGTGG TTTTTCTTTT CACCAGTGAG ACGGGCAACA GCTGATTGCC CTTCACCGCC
  241 TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG GCGAAAATCC
  301 TGTTTGATGG TGGTTAACGG CGGGATATAA CATGAGCTGT CTTCGGTATC GTCGTATCCC
  361 ACTACCGAGA TATCCGCACC AACGCGCAGC CCGGACTCGG TAATGGCGCG CATTGCGCCC
  421 AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA CGATGCCCTC ATTCAGCATT
  481 TGCATGGTTT GTTGAAAACC GGACATGGCA CTCCAGTCGC CTTCCCGTTC CGCTATCGGC
  541 TGAATTTGAT TGCGAGTGAG ATATTTATGC CAGCCAGCCA GACGCAGACG CGCCGAGACA
  601 GAACTTAATG GGCCCGCTAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC CAGATGCTCC
  661 ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC TGTTGATGGG TGTCTGGTCA
  721 GAGACATCAA GAAATAACGC CGGAACATTA GTGCAGGCAG CTTCCACAGC AATGGCATCC
  781 TGGTCATCCA GCGGATAGTT AATGATCAGC CCACTGACGC GTTGCGCGAG AAGATTGTGC
  841 ACCGCCGCTT TACAGGCTTC GACGCCGCTT CGTTCTACCA TCGACACCAC CACGCTGGCA
  901 CCCAGTTGAT CGGCGTGGAG ATTTAATCGC CGCGACAATT TGCGACGGCG CGTTGCAGGG
  961 CCAGACTGGG ATGTGGCAAC GCCAATCAGC AACGACTGTT GCCCGCCCAG TTGTGTGCCA
 1021 CGCGTTGGGG AATGTAAATC AGCCTCCGCC CATCGCGGCT TCAACTTTTT CCCGCGTTTT
1081 TCGCAGAATC GTGGCTTGCC CTGGTTCAAC ACGTCGGGGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Mizuno, H., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011).

2019.07.30

GNP_filter3_RDBDEP_html_190432.pl