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pET28/Dronpa-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.

Catalog number RDB15470
Resource name pET28/Dronpa-NT-Lys
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
Vector backbone Plasmid
Gene/insert name Lysenin
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15470hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB15470_B4Eup1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment]  [Bank Transfer Payment]
MTA, for use for not-for-profit academic purpose [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Mizuno, H., Abe, M., Dedecker, P., Makino, A., Rocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A. (2011) Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
提供案内 (日本国内) [open/close]

提供依頼書
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
宮脇敦史博士による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Mizuno, H., Abe, M., Dedecker, P., Makino, A., Rocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A. (2011) Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
付加的提供条件:
本件リソースの使用は学術機関での学術研究に限る。
備考 提供条件は英文のまま記載してください。
宮脇敦史博士の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15470 pET28/Dronpa-NT-Lys DNA solution


How to cite this biological resource

Materials & Methods section:

The pET28/Dronpa-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15470).

Reference section:

Mizuno, H., Abe, M., Dedecker, P., ARocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011). [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Mizuno, H., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011). [link to RRC of NBRP]