Resource data sheet
Prev.

GEPRA-B

Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-beta.

Catalog number RDB15249
Resource name GEPRA-B
Clone info. Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-beta.
Vector backbone pCS2+ (plasmid)
Gene/insert name mouse RARbeta cDNA Aequorea victoria GFP cDNA
Depositor Miyawaki, Atsushi |
 
Other clones in our bank mouse Rarb (NCBI Gene 218772) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Approval form by depositor [Word]
Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15249 GEPRA-B DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15249_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F RDB15249_A7H9a.seq
>D04498F7_2_A7H9_pCMV-IE94_F_B05_05_ABI24.ab1
    1 AAGGGCGGGT AGCGTGCCTA ATGGGAGGTC TATATAAGCA ATGCTCGTTT AGGGAACCGC
   61 CATTCTGCCT GGGGACGTCG GAGCAAGCTT GATTTAGGTG ACACTATAGA ATACAAGCTA
  121 CTTGTTCTTT TTGCAGGATC CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT
  181 GCCCATCCTG GTCGAGCTGC AGGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA
  241 GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA
  301 GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGAGC TGGGGCGTGC AGTGCTTCAG
  361 CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGTCATGC CCGAAGGCTA
  421 CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT
  481 GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA
  541 GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC ATCAGCCACA ACGTCTATAT
  601 CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGT
  661 GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC
  721 CGTGAACCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA
  781 CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCCGCA TGCATGAGAG
  841 CTATGAGATG ACAGCGGAGC TAGACGACCT CACTGAGAAG ATCCGGAAAG CCCACCAGGA
  901 AACCTTTCCC TCACTCTGCC AGCTGGGTAA ATACACCACG AATTCCAGCG CTGACCACCG
  961 GGTCCGATTG GACTTGGGCC CTCTGGGACA AATTCAGTGA GCTGGGCCAC CAAGTGCATT
 1021 TATTTAGATC GTGGAGTTTC GTAGCGTCTG CCGGGCTTTC ACAGGTCTGA CATCGCAGAC
1081 AGATCACCTT GCTCAAGGCG GCTGCCTTGC ATATTCTTGG ATTTCCTCAG AATTTGTA
//
Primer: pAxCALNL_F1 RDB15249_A7H9b.seq
>D04498F7_2_A7H9_pAxCALNL_F1_A05_02_ABI24.ab1
    1 ACAAGCATCT TATCATGTCT GGATCTACGT AATACGACTC ACTATAGTTC TAGAGGCTCG
   61 AGTTACTTGT ACAGCTCGTT CATGCCTTCA GTGATCCCGG CGGCGGTCAG GAACTCCAGC
  121 AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGAACA GGGCGGACTG GTAGCTCAGG
  181 TAGTGGTTGT CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG
  241 TCGGCGAGCT GCACGCCGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG
  301 CCGTTCTTCT GCTTGTCGGC GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC
  361 TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC
  421 ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG
  481 AAGAAGATGG TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC
  541 TGCTTCATGT GGTCGGGGTA GCGGGCGAAG CACTGCAGGC CGTAGCCCAG GGTGGTCACG
  601 AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA GCAGCTTCAG GGTCAGCTTG
  661 CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG
  721 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC
  781 ATGAGCTCTT CATGTCCTTC AGAATTCTCC AGCATTTCCT GAATGAGAGG TGGCATTGAT
  841 CCAGGAATTT CCATTTTCAA GGTAATTACA CGTTCGGCAC CTTTCGCGCT GATGCTGCGG
  901 AGATCTGTGA TTTTCATTAA GATCTTTGGA AACATGTGAG GCTTGCTGGG TCGTCGTTTT
  961 CTAATGTAAA TCTTTAGTGC TTCCAGCAGT GGCTTCTTGG AGCTTGTCTA CTTTTGTGGT
 1021 TCTCAGGTCC TGGCGGGTCT CACAGATTAA ACAGATGGGC ACTGAGAGGC TGCTTTCTGT
 1081 GTCATCATTT CGAAAGGCAG AGCTGTTGCC AAAGTGACAC AGGGTTCAGT CAGGAAGGAT
1141 CT
//
Primer: RARbeta_LBD RDB15249_A7H9c.seq
>D04498F7_2_A7H9_RARbeta_LBD_C05_08_ABI24.ab1
    1 CGGAGGAAGA GGTGGCATTG ATCCAGGATT TCCATTTTCA AGGTAATTAC ACGTTCGGCA
   61 CCTTTCGCGC TGATGCTGCG GAGATCTGTG ATTTTCATTA AGATCTTTGG AAACATGTGA
  121 GGCTTGCTGG GTCGTCGTTT TCTAATGTAA ATCTTTAGTG CTTCCAGCAG TGGTTCTTGG
  181 AGCTTGTCTA CTTTTGTTGG TTCCTCAAGG TCCTGGCGGT CTCCACAGAT TAAACAGATG
  241 GCACTGAGAA GGCCTGTTTC TGTGTCATCC ATTTCCAAAG GCAGGAGCTG GTTGGCAAAG
  301 GTGAACACAA GGTCAGTCAG AGGACCGAAG CCAGCATTGT GCATCTGAGT TCGATTTAGT
  361 GTAAGGCCAT CAGAGAAAGT CATGGTGTCT TGCTCTGGGG TATACCTGGT ACAAATTCTG
  421 AGAATCAAGA TATCCAAGCA GGCGGCTTTG AGCAGGGTGA TCTGGTCTGC GATGGTCAGA
  481 CCTGTGAAGC CCGGCAGACG CTTGGCGAAC TCCACGATCT TAATAATGCA CTTGGTGGCC
  541 AGCTCACTGA ATTTGTCCCA GAGGCCCAAG TCCAATCGGA CCCGGTGGTC AGCGCTGGAA
  601 TTCGTGGTGT ATTTACCCAG CTGGCAGAGT GAGGGAAAGG TTTCCTGGTG GGCTTTCCGG
  661 ATCTTCTCAG TGAGGTCGTC TAGCTCCGCT GTCATCTCAT AGCTCTCATG CATGCGGGCG
  721 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG
  781 GCGGACTGGG TGCTCAGGTA GTGGTTGTCG GGCAGGTTCA CGGGGCCGTC GCCGATGGGG
  841 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCACGATGTT GTGGCGGATC
  901 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG
  961 ATGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTTGCCC GTCCTCCTTG AAGTCGATGC
 1021 CCTTTCAGCT CGATGCGATC ACAGGGTGTC GCCCTCGACC TCAACTCGGC GCGGGTCTTG
 1081 TAGCTGCGTT CGTTCATGGA GAGATGCAGC GCTCCTGACG ATAGCTCATC GGGTCAAGTG
1141 AAACG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268.
reference Schilling, T.F., Visualizing retinoic acid morphogen gradients. Methods Cell Biol. 133: 139-163 (2016). PMID 27263412.

2019.07.04

GNP_filter3_RDBDEP_html_190431.pl