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mt-mKeima (h)/pIND(SP1)

Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.

Catalog number RDB15238
Resource name mt-mKeima (h)/pIND(SP1)
Clone info. Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.
The expression of mt-mKeima(h) from this vector requires cotransfection of the activator plasmid pVgRXR or an equivalent, and treatment with an ecdysone analog such as Ponasterone A.
Vector backbone pIND (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name Montipora. sp RFP cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15238 mt-mKeima (h)/pIND(SP1) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Organelle marker (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15238_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUAST3' RDB15238_A7H9a.seq
>D04498E5-1_A7H9_pUAST3'_F03_18_ABI24.ab1
    1 AATGCAGAGT ATTATTGAAT ACAAGAAGAG AACTCTGAAT ACTTTCAACA AGTTACCGAG
   61 AAAGAAGAAC TCACACACAG CTAGCGTTTA AACTTAAGCT TGGTACCATG TCCGTCCTGA
  121 CGCCGCTGCT GCTGCGGGGC TTGACAGGCT CGGCCCGGCG GCTCCCAGTG CCGCGCGCCA
  181 AGATCCATTC GTTGCCGCCG GAGGGGAAGC TTGGGATGTC CGTCCTGACG CCGCTGCTGC
  241 TGCGGGGCTT GACAGGCTCG GCCCGGCGGC TCCCAGTGCC GCGCGCCAAG ATCCATTCGT
  301 TGCCGCCGGA GGGGAAGCTC GGGGGATCCG CCATGGTGAG CGTGATCGCC AAGCAGATGA
  361 CCTACAAGGT GTACATGAGC GGCACCGTGA ACGGCCACTA CTTCGAGGTG GAGGGCGACG
  421 GCAAGGGCAA GCCCTACGAG GGCGAGCAGA CCGTGAAGCT GACCGTGACC AAGGGTGGCC
  481 CCCTGCCCTT CGCCTGGGAC ATCCTGAGCC CCCAGCTCCA GTACGGCAGC ATCCCCTTCA
  541 CCAAGTACCC CGAGGACATC CCCGACTACT TCAAGCAGAG CTTCCCCGAG GGCTACACCT
  601 GGGAGCGCAG CATGAACTTC GAGGACGGCG CCGTGTGCAC CGTGAGCAAC GACAGCAGCA
  661 TCCAGGGCAA CTGCTTCATC TACAACGTGA AGATCAGCGG CGAGAACTTC CCCCCCAACG
  721 GCCCCGTGAT GCAGAAGAAG ACCCAGGGCT GGGAGCCCAG CACCGAGCGC CTGTTCGCCC
  781 GCGACGGAAT GCTGATCGGC AACGACTACA TGGCCCTGAA GCTGGAGGGC GGCGGCCACT
  841 ACCTGTGCGA GTTCAAGAGC ACCTACAAGG CCAAGAAGCC CGTGAGGATG CCCGGCCGCC
  901 ACGAGATCGA CCGCAAGCTG GACGTGACCA GCCACAACCG CGACTACACC AGCGTGGAGC
  961 AGTGCGAGAT CGCCATCGCC CGCCACTCCC TGCTGGGCTA AGAATTTCTG CAGATATCCA
 1021 GCACAGTGGG CGGGCCGCTC GAGTCTAGAG GGCCCGGTTT AAACCCGCTG ATCAGCCCTC
1081 GAACTGTGCG TCTAGGTTTG CCAGGCCATT CTGATTGTTT GCCCCCCATG C
//
Primer: BGH_rev2 RDB15238_A7H9b.seq
>D04498E5-1_A7H9_BGH_rev2_G03_21_ABI24.ab1
    1 CTTCAGTTCA GGAGGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG CCACTGTGCT
  121 GGATATCTGC AGAATTCTTA GCCCAGCAGG GAGTGGCGGG CGATGGCGAT CTCGCACTGC
  181 TCCACGCTGG TGTAGTCGCG GTTGTGGCTG GTCACGTCCA GCTTGCGGTC GATCTCGTGG
  241 CGGCCGGGCA TCCTCACGGG CTTCTTGGCC TTGTAGGTGC TCTTGAACTC GCACAGGTAG
  301 TGGCCGCCGC CCTCCAGCTT CAGGGCCATG TAGTCGTTGC CGATCAGCAT TCCGTCGCGG
  361 GCGAACAGGC GCTCGGTGCT GGGCTCCCAG CCCTGGGTCT TCTTCTGCAT CACGGGGCCG
  421 TTGGGGGGGA AGTTCTCGCC GCTGATCTTC ACGTTGTAGA TGAAGCAGTT GCCCTGGATG
  481 CTGCTGTCGT TGCTCACGGT GCACACGGCG CCGTCCTCGA AGTTCATGCT GCGCTCCCAG
  541 GTGTAGCCCT CGGGGAAGCT CTGCTTGAAG TAGTCGGGGA TGTCCTCGGG GTACTTGGTG
  601 AAAGGGGATG CTGCCGTACT GGAGCTGGGG GCTCAGATGT CCCAGGCGAA GGCAGGGGCC
  661 ACCCTTGGTC ACGTCAGCTT CACGGTCTGC TCGCCCTCGT AGGGCTTGCC CTTGCCGTCG
  721 CCCCTCCACC TCGAAGTAGT GGCCGTTCAC GGTGCCGCTC ATGTACACCT TGTAGGTCAT
  781 CTGCTGGCGA TCACCGCTCA CCATGGCGGA TCCCCGAGCT TTCCCCTCCG CGGCACGAAT
  841 GATCTTGGCC GCGCGCACTG GGAGCCGCCG GTCGAGCTTG TCAAGCCCGC AGCACACGGG
  901 TCAGACGACA TCCAGCTTCC CTCGGGGCAC GATGGATCTG GCGCGCGCAC TGGGAGCCTC
  961 GGTAGCCTGT CAAGCTCGAC ACACAACGGG CTTGAGCGAT GTACAAGCTA GTAACGCACC
1021 GTCGTGTGGA TTCTCTTTGC
//
Primer: SV40pro_F RDB15238_A7H9c.seq
>D04498E5-1_A7H9_SV40pro_F_H03_24_ABI24.ab1
    1 GCCTTGTCCT TGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCCT GCCATCACGA GATTTCGATT CCAACCGCCC GCCCTTCTAT GAAAAGTTGG
 1021 GGCTTTCGGA ATCGTTTTCC GGGACGCCGG CTTGGATGAT CCCTCCAGCG CGGGGAATCT
 1081 CATGCTTGGA GTTTCATCGC CACCCCACCT GCTATTGCCA GCTTATAATG GGTTTACACA
1141 AT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.
reference Marcassa, E., Dual role of USP30 in controlling basal pexophagy and mitophagy. EMBO Rep. 19 (7). pii: e45595 (2018). PMID 29895712.
reference Sun, N., A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima. Nat. Protoc. 12 (8): 1576-1587 (2017). PMID 28703790.
reference Lazarou, M., The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature 524 (7565): 309-314 (2015). PMID 26266977.

2019.07.10

GNP_filter3_RDBDEP_html_190431.pl