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mt-mKeima (h)/pIND(SP1)

Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.

Catalog number RDB15238
Resource name mt-mKeima (h)/pIND(SP1)
Clone info. Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.
Comment The expression of mt-mKeima(h) from this vector requires cotransfection of the activator plasmid pVgRXR or an equivalent, and treatment with an ecdysone analog such as Ponasterone A.
Vector backbone pIND (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name Montipora. sp RFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Montipora. sp RFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15238 mt-mKeima (h)/pIND(SP1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualization of Organelles (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15238_A7H9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUAST3'
            Sequence file: RDB15238_A7H9a.seq check
            >D04498E5-1_A7H9_pUAST3'_F03_18_ABI24.ab1
                1 AATGCAGAGT ATTATTGAAT ACAAGAAGAG AACTCTGAAT ACTTTCAACA AGTTACCGAG
               61 AAAGAAGAAC TCACACACAG CTAGCGTTTA AACTTAAGCT TGGTACCATG TCCGTCCTGA
              121 CGCCGCTGCT GCTGCGGGGC TTGACAGGCT CGGCCCGGCG GCTCCCAGTG CCGCGCGCCA
              181 AGATCCATTC GTTGCCGCCG GAGGGGAAGC TTGGGATGTC CGTCCTGACG CCGCTGCTGC
              241 TGCGGGGCTT GACAGGCTCG GCCCGGCGGC TCCCAGTGCC GCGCGCCAAG ATCCATTCGT
              301 TGCCGCCGGA GGGGAAGCTC GGGGGATCCG CCATGGTGAG CGTGATCGCC AAGCAGATGA
              361 CCTACAAGGT GTACATGAGC GGCACCGTGA ACGGCCACTA CTTCGAGGTG GAGGGCGACG
              421 GCAAGGGCAA GCCCTACGAG GGCGAGCAGA CCGTGAAGCT GACCGTGACC AAGGGTGGCC
              481 CCCTGCCCTT CGCCTGGGAC ATCCTGAGCC CCCAGCTCCA GTACGGCAGC ATCCCCTTCA
              541 CCAAGTACCC CGAGGACATC CCCGACTACT TCAAGCAGAG CTTCCCCGAG GGCTACACCT
              601 GGGAGCGCAG CATGAACTTC GAGGACGGCG CCGTGTGCAC CGTGAGCAAC GACAGCAGCA
              661 TCCAGGGCAA CTGCTTCATC TACAACGTGA AGATCAGCGG CGAGAACTTC CCCCCCAACG
              721 GCCCCGTGAT GCAGAAGAAG ACCCAGGGCT GGGAGCCCAG CACCGAGCGC CTGTTCGCCC
              781 GCGACGGAAT GCTGATCGGC AACGACTACA TGGCCCTGAA GCTGGAGGGC GGCGGCCACT
              841 ACCTGTGCGA GTTCAAGAGC ACCTACAAGG CCAAGAAGCC CGTGAGGATG CCCGGCCGCC
              901 ACGAGATCGA CCGCAAGCTG GACGTGACCA GCCACAACCG CGACTACACC AGCGTGGAGC
              961 AGTGCGAGAT CGCCATCGCC CGCCACTCCC TGCTGGGCTA AGAATTTCTG CAGATATCCA
             1021 GCACAGTGGG CGGGCCGCTC GAGTCTAGAG GGCCCGGTTT AAACCCGCTG ATCAGCCCTC
            1081 GAACTGTGCG TCTAGGTTTG CCAGGCCATT CTGATTGTTT GCCCCCCATG C
            //
            Primer: BGH_rev2
            Sequence file: RDB15238_A7H9b.seq check
            >D04498E5-1_A7H9_BGH_rev2_G03_21_ABI24.ab1
                1 CTTCAGTTCA GGAGGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA
               61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG CCACTGTGCT
              121 GGATATCTGC AGAATTCTTA GCCCAGCAGG GAGTGGCGGG CGATGGCGAT CTCGCACTGC
              181 TCCACGCTGG TGTAGTCGCG GTTGTGGCTG GTCACGTCCA GCTTGCGGTC GATCTCGTGG
              241 CGGCCGGGCA TCCTCACGGG CTTCTTGGCC TTGTAGGTGC TCTTGAACTC GCACAGGTAG
              301 TGGCCGCCGC CCTCCAGCTT CAGGGCCATG TAGTCGTTGC CGATCAGCAT TCCGTCGCGG
              361 GCGAACAGGC GCTCGGTGCT GGGCTCCCAG CCCTGGGTCT TCTTCTGCAT CACGGGGCCG
              421 TTGGGGGGGA AGTTCTCGCC GCTGATCTTC ACGTTGTAGA TGAAGCAGTT GCCCTGGATG
              481 CTGCTGTCGT TGCTCACGGT GCACACGGCG CCGTCCTCGA AGTTCATGCT GCGCTCCCAG
              541 GTGTAGCCCT CGGGGAAGCT CTGCTTGAAG TAGTCGGGGA TGTCCTCGGG GTACTTGGTG
              601 AAAGGGGATG CTGCCGTACT GGAGCTGGGG GCTCAGATGT CCCAGGCGAA GGCAGGGGCC
              661 ACCCTTGGTC ACGTCAGCTT CACGGTCTGC TCGCCCTCGT AGGGCTTGCC CTTGCCGTCG
              721 CCCCTCCACC TCGAAGTAGT GGCCGTTCAC GGTGCCGCTC ATGTACACCT TGTAGGTCAT
              781 CTGCTGGCGA TCACCGCTCA CCATGGCGGA TCCCCGAGCT TTCCCCTCCG CGGCACGAAT
              841 GATCTTGGCC GCGCGCACTG GGAGCCGCCG GTCGAGCTTG TCAAGCCCGC AGCACACGGG
              901 TCAGACGACA TCCAGCTTCC CTCGGGGCAC GATGGATCTG GCGCGCGCAC TGGGAGCCTC
              961 GGTAGCCTGT CAAGCTCGAC ACACAACGGG CTTGAGCGAT GTACAAGCTA GTAACGCACC
            1021 GTCGTGTGGA TTCTCTTTGC
            //
            Primer: SV40pro_F
            Sequence file: RDB15238_A7H9c.seq check
            >D04498E5-1_A7H9_SV40pro_F_H03_24_ABI24.ab1
                1 GCCTTGTCCT TGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
               61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
              121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
              181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
              241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
              301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
              361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
              421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
              481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
              541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
              601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
              661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
              721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
              781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
              841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
              901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
              961 GCCCAACCCT GCCATCACGA GATTTCGATT CCAACCGCCC GCCCTTCTAT GAAAAGTTGG
             1021 GGCTTTCGGA ATCGTTTTCC GGGACGCCGG CTTGGATGAT CCCTCCAGCG CGGGGAATCT
             1081 CATGCTTGGA GTTTCATCGC CACCCCACCT GCTATTGCCA GCTTATAATG GGTTTACACA
            1141 AT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.
            reference Marcassa, E., Dual role of USP30 in controlling basal pexophagy and mitophagy. EMBO Rep. 19 (7). pii: e45595 (2018). PMID 29895712.
            reference Sun, N., A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima. Nat. Protoc. 12 (8): 1576-1587 (2017). PMID 28703790.
            reference Lazarou, M., The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature 524 (7565): 309-314 (2015). PMID 26266977.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl