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mt-mKeima (h)/pIND(SP1) (#RDB15238)

Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.

Clone info. Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.
Comment The expression of mt-mKeima(h) from this vector requires cotransfection of the activator plasmid pVgRXR or an equivalent, and treatment with an ecdysone analog such as Ponasterone A.
Vector backbone pIND (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name Montipora. sp RFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15238hts01.seq checkAssembled from experimentally sequenced data.
Publication Katayama, H., Chem. Biol. 18 (8): 1042-1052 (2011). [link to RRC of NBRP]
Test sheet RDB15238_A8Gcp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Katayama, H. et al., Chemistry & Biology 18 (8): 1042-1052 (2011).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Katayama, H. et al., Chemistry & Biology 18 (8): 1042-1052 (2011).
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15238 mt-mKeima (h)/pIND(SP1) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The mt-mKeima (h)/pIND(SP1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15238).

Reference section:

Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. [link to RRC of NBRP]
reference Marcassa, E., Dual role of USP30 in controlling basal pexophagy and mitophagy. EMBO Rep. 19 (7). pii: e45595 (2018). PMID 29895712.
reference Sun, N., A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima. Nat. Protoc. 12 (8): 1576-1587 (2017). PMID 28703790.
reference Lazarou, M., The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature 524 (7565): 309-314 (2015). PMID 26266977.