Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.
Clone info. | Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal. |
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Comment | The expression of mt-mKeima(h) from this vector requires cotransfection of the activator plasmid pVgRXR or an equivalent, and treatment with an ecdysone analog such as Ponasterone A. |
Vector backbone | pIND (Plasmid) |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell). |
Gene/insert name | Montipora. sp RFP cDNA |
Depositor|Developer | Miyawaki, Atsushi | |
Sequence (full) | RDB15238hts01.seq ![]() |
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Publication | Katayama, H., Chem. Biol. 18 (8): 1042-1052 (2011). [link to RRC of NBRP] |
Test sheet | RDB15238_A8Gcp1-2.pdf ![]() |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Katayama, H. et al., Chemistry & Biology 18 (8): 1042-1052 (2011). Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Katayama, H. et al., Chemistry & Biology 18 (8): 1042-1052 (2011). 付加的使用条件: 本件リソースの使用は学術機関での学術研究に限る。 |
備考 | 提供条件は英文のまま記載してください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15238 | mt-mKeima (h)/pIND(SP1) | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The mt-mKeima (h)/pIND(SP1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15238). |
Reference section:
Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. [link to RRC of NBRP] |
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reference | Marcassa, E., Dual role of USP30 in controlling basal pexophagy and mitophagy. EMBO Rep. 19 (7). pii: e45595 (2018). PMID 29895712. |
reference | Sun, N., A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima. Nat. Protoc. 12 (8): 1576-1587 (2017). PMID 28703790. |
reference | Lazarou, M., The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature 524 (7565): 309-314 (2015). PMID 26266977. |
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