RDB15238 - mt-mKeima (h)/pIND(SP1)

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icon below as well as clone information before placing your order.

mt-mKeima (h)/pIND(SP1) (#RDB15238)

Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.

Clone info.	Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.
Comment	The expression of mt-mKeima(h) from this vector requires cotransfection of the activator plasmid pVgRXR or an equivalent, and treatment with an ecdysone analog such as Ponasterone A.
Vector backbone	pIND (Plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name	Montipora. sp RFP cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15238	mt-mKeima (h)/pIND(SP1)	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mt-mKeima (h)/pIND(SP1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15238).

Reference section:

Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15238_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUAST3' () Sequence file: RDB15238_A7H9a.seq
>D04498E5-1_A7H9_pUAST3'_F03_18_ABI24.ab1 1 AATGCAGAGT ATTATTGAAT ACAAGAAGAG AACTCTGAAT ACTTTCAACA AGTTACCGAG 61 AAAGAAGAAC TCACACACAG CTAGCGTTTA AACTTAAGCT TGGTACCATG TCCGTCCTGA 121 CGCCGCTGCT GCTGCGGGGC TTGACAGGCT CGGCCCGGCG GCTCCCAGTG CCGCGCGCCA 181 AGATCCATTC GTTGCCGCCG GAGGGGAAGC TTGGGATGTC CGTCCTGACG CCGCTGCTGC 241 TGCGGGGCTT GACAGGCTCG GCCCGGCGGC TCCCAGTGCC GCGCGCCAAG ATCCATTCGT 301 TGCCGCCGGA GGGGAAGCTC GGGGGATCCG CCATGGTGAG CGTGATCGCC AAGCAGATGA 361 CCTACAAGGT GTACATGAGC GGCACCGTGA ACGGCCACTA CTTCGAGGTG GAGGGCGACG 421 GCAAGGGCAA GCCCTACGAG GGCGAGCAGA CCGTGAAGCT GACCGTGACC AAGGGTGGCC 481 CCCTGCCCTT CGCCTGGGAC ATCCTGAGCC CCCAGCTCCA GTACGGCAGC ATCCCCTTCA 541 CCAAGTACCC CGAGGACATC CCCGACTACT TCAAGCAGAG CTTCCCCGAG GGCTACACCT 601 GGGAGCGCAG CATGAACTTC GAGGACGGCG CCGTGTGCAC CGTGAGCAAC GACAGCAGCA 661 TCCAGGGCAA CTGCTTCATC TACAACGTGA AGATCAGCGG CGAGAACTTC CCCCCCAACG 721 GCCCCGTGAT GCAGAAGAAG ACCCAGGGCT GGGAGCCCAG CACCGAGCGC CTGTTCGCCC 781 GCGACGGAAT GCTGATCGGC AACGACTACA TGGCCCTGAA GCTGGAGGGC GGCGGCCACT 841 ACCTGTGCGA GTTCAAGAGC ACCTACAAGG CCAAGAAGCC CGTGAGGATG CCCGGCCGCC 901 ACGAGATCGA CCGCAAGCTG GACGTGACCA GCCACAACCG CGACTACACC AGCGTGGAGC 961 AGTGCGAGAT CGCCATCGCC CGCCACTCCC TGCTGGGCTA AGAATTTCTG CAGATATCCA 1021 GCACAGTGGG CGGGCCGCTC GAGTCTAGAG GGCCCGGTTT AAACCCGCTG ATCAGCCCTC 1081 GAACTGTGCG TCTAGGTTTG CCAGGCCATT CTGATTGTTT GCCCCCCATG C //
Primer: BGH_rev2 () Sequence file: RDB15238_A7H9b.seq
>D04498E5-1_A7H9_BGH_rev2_G03_21_ABI24.ab1 1 CTTCAGTTCA GGAGGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA 61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG CCACTGTGCT 121 GGATATCTGC AGAATTCTTA GCCCAGCAGG GAGTGGCGGG CGATGGCGAT CTCGCACTGC 181 TCCACGCTGG TGTAGTCGCG GTTGTGGCTG GTCACGTCCA GCTTGCGGTC GATCTCGTGG 241 CGGCCGGGCA TCCTCACGGG CTTCTTGGCC TTGTAGGTGC TCTTGAACTC GCACAGGTAG 301 TGGCCGCCGC CCTCCAGCTT CAGGGCCATG TAGTCGTTGC CGATCAGCAT TCCGTCGCGG 361 GCGAACAGGC GCTCGGTGCT GGGCTCCCAG CCCTGGGTCT TCTTCTGCAT CACGGGGCCG 421 TTGGGGGGGA AGTTCTCGCC GCTGATCTTC ACGTTGTAGA TGAAGCAGTT GCCCTGGATG 481 CTGCTGTCGT TGCTCACGGT GCACACGGCG CCGTCCTCGA AGTTCATGCT GCGCTCCCAG 541 GTGTAGCCCT CGGGGAAGCT CTGCTTGAAG TAGTCGGGGA TGTCCTCGGG GTACTTGGTG 601 AAAGGGGATG CTGCCGTACT GGAGCTGGGG GCTCAGATGT CCCAGGCGAA GGCAGGGGCC 661 ACCCTTGGTC ACGTCAGCTT CACGGTCTGC TCGCCCTCGT AGGGCTTGCC CTTGCCGTCG 721 CCCCTCCACC TCGAAGTAGT GGCCGTTCAC GGTGCCGCTC ATGTACACCT TGTAGGTCAT 781 CTGCTGGCGA TCACCGCTCA CCATGGCGGA TCCCCGAGCT TTCCCCTCCG CGGCACGAAT 841 GATCTTGGCC GCGCGCACTG GGAGCCGCCG GTCGAGCTTG TCAAGCCCGC AGCACACGGG 901 TCAGACGACA TCCAGCTTCC CTCGGGGCAC GATGGATCTG GCGCGCGCAC TGGGAGCCTC 961 GGTAGCCTGT CAAGCTCGAC ACACAACGGG CTTGAGCGAT GTACAAGCTA GTAACGCACC 1021 GTCGTGTGGA TTCTCTTTGC //
Primer: SV40pro_F () Sequence file: RDB15238_A7H9c.seq
>D04498E5-1_A7H9_SV40pro_F_H03_24_ABI24.ab1 1 GCCTTGTCCT TGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC 121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT 661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC 961 GCCCAACCCT GCCATCACGA GATTTCGATT CCAACCGCCC GCCCTTCTAT GAAAAGTTGG 1021 GGCTTTCGGA ATCGTTTTCC GGGACGCCGG CTTGGATGAT CCCTCCAGCG CGGGGAATCT 1081 CATGCTTGGA GTTTCATCGC CACCCCACCT GCTATTGCCA GCTTATAATG GGTTTACACA 1141 AT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. [link to RRC of NBRP]
reference	Marcassa, E., Dual role of USP30 in controlling basal pexophagy and mitophagy. EMBO Rep. 19 (7). pii: e45595 (2018). PMID 29895712.
reference	Sun, N., A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima. Nat. Protoc. 12 (8): 1576-1587 (2017). PMID 28703790.
reference	Lazarou, M., The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature 524 (7565): 309-314 (2015). PMID 26266977.

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External Database
Montipora. sp RFP

Reference sequence