Calcium ion sensor clone Ratiometric pericam having a circularly permuted YFP and a CaM.
Catalog number | RDB15143 |
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Resource name | Ratiometric pericam/pcDNA3 |
Clone info. | Calcium ion sensor clone Ratiometric pericam having a circularly permuted YFP and a CaM. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition. |
Vector backbone | pcDNA3 (plasmid) |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells) |
Gene/insert name | X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Miyawaki, Atsushi | |
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Other clones in our bank | X. laevis (KEGG orthology K02183) | synthetic (KEGG orthology K00907) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() Schedule A (GFP-A) [Link] Approval form [Word] ![]() |
Remarks | Please use Schedule A (GFP-A) additionally. |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB15143 | Ratiometric pericam/pcDNA3 | Under QC test. Please contact us. | DNA solution |
Electronic file
Original reference
original | Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. |
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Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Calcium Ion Sensor Clones (English text) |
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Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. |
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reference | Fukano, T., Fast dual-excitation ratiometry with light-emitting diodes and high-speed liquid crystal shutters. Biochem. Biophys. Res. Commun. 3340 (1): 250-255 (2006). PMID 16360639. |
reference | Fukano, T., Simultaneous dual-excitation ratiometry using orthogonal linear polarized lights. Biochem. Biophys. Res. Commun. 317 (1): 77-83 (2004). PMID 15047150. |
2020.12.17
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