RDB15143 - Ratiometric pericam/pcDNA3

Calcium ion sensor clone Ratiometric pericam having a circularly permuted YFP and a CaM.

Distribution information

Catalog number	RDB15143
Resource name	Ratiometric pericam/pcDNA3
Clone info.	Calcium ion sensor clone Ratiometric pericam having a circularly permuted YFP and a CaM. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition.
Vector backbone	pcDNA3 (plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name	X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Other clones in our bank	X. laevis (KEGG orthology K02183) \| synthetic (KEGG orthology K00907) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users)

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 Schedule A（GFP-A, 英語版のみ）が追加で必要です。

References and tips

Sequence information

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB15143	Ratiometric pericam/pcDNA3	Under QC test. Please contact us.	DNA solution

original	Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

Featured content	Calcium Ion Sensor Clones (English text)

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

original	Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.
reference	Fukano, T., Fast dual-excitation ratiometry with light-emitting diodes and high-speed liquid crystal shutters. Biochem. Biophys. Res. Commun. 3340 (1): 250-255 (2006). PMID 16360639.
reference	Fukano, T., Simultaneous dual-excitation ratiometry using orthogonal linear polarized lights. Biochem. Biophys. Res. Commun. 317 (1): 77-83 (2004). PMID 15047150.