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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Ratiometric pericam/pcDNA3

Calcium ion sensor clone Ratiometric pericam having a circularly permuted YFP and a CaM.

Catalog number RDB15143
Resource name Ratiometric pericam/pcDNA3
Clone info. Calcium ion sensor clone Ratiometric pericam having a circularly permuted YFP and a CaM. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Schedule A (GFP-A) [Link]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please use Schedule A (GFP-A) additionally.
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        Schedule A(GFP-A, 英語版のみ) [Link]
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件は英文のまま記載してください。
        Schedule A(GFP-A, 英語版のみ)が追加で必要です。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15143 Ratiometric pericam/pcDNA3 DNA solution

        Ordering Information [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15143_A7H9p1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward
        Sequence file: RDB15143_A7H9a.seq check
        >D04498D1_A7H9_1-CMV-Forward_G07_19_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NNNNGGCGGT AGGCNNTGTA NGGTGNNNNG
           61 TCTATATAAG CAGAGCTCTC TGGCTAACTA NANANCCCAC TGCTTACTGG CTTATCGAAA
          121 TTAATACGAC TCACTATAGG GAGACCCAAG CTTGGTACCG AGCTCGGATC CACCATGAAG
          181 AGGCGCTGGA AGAAAAACTT CATTGCCGTC AGCGCTGCCA ACCGGTTCAA GAAGATCTCC
          241 AGCTCCGGGG CACTGGGGTC TGCAGGCTAC AACAGCGACA ACGTCTATAT CATGGCCGAC
          301 AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC
          361 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG
          421 CCCGACAACC ACTACCTGAG CTTCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC
          481 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG
          541 CTGTACAAGG TCGACGGTGG CAGCGGTGGC ACCGGTGTGA GCAAGGGCGA GGAGCTGTTC
          601 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC
          661 GTGTCCGGCG AGGGCGNNGG CGATGCCACC TACGGCAAGC TGACCCTGAA NNNCNNNNGC
          721 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCNNGA CCACCTTCGG CTACGGCCTG
          781 AANTGCTTCN CCCGCTACCC CGACCACATG AAGCANCANN ACTTCTTCAA NNTCCGCCAT
          841 GCNNNNNGGC TACNTCCANG NANCGCACCA TCTTCTNNAA GGACNANGGC NANNNNCNAG
          901 ACCCGCGCCN ANNNNAAGTT CNAGGGCGAN ANCCTGGTGA ANNNCNTCNN NCTNANNGGC
          961 ATCGNNNTNN ANGNNNNANN GGCNACNNNC NGGGGCNNAA NNCTNGNNNN TANNANNGGT
        1021 ACCNNANCNN NCNNNNNNNA NNANNNNANN NNNNNNNN
        //
        Primer: BGH_rev2
        Sequence file: RDB15143_A7H9b.seq check
        >D04498D1_A7H9_1-BGH_rev2_H07_22_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN GGNNGNNGGG NAAACNACAG ATGGCTGGCA ACTAGAAGGC
           61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT
          121 AGATGCATGC TCGAGCGGCC GCCAGTGTGA TGGATATCTG CAGAATTCTT ACTTTGCTGT
          181 CATCATTTGT ACAAACTCTT CATAGTTTAC TTGGCCATCA CCATCGATAT CTGCTTCCCT
          241 TATCATTTCA TCAACTTCTT CATCTGTTAA CTTCTCCCCG AGGTTTGTCA TGACGTGACG
          301 TAACTGAGCA GCGCTGATGT AGCCGTTCCC ATCCTTGTCA AAAACACGGA ATGCTTCTCG
          361 GATTTCCTCT TCGCTGTCTG TGTCCTTCAT TTTTCTAGCC ATCATAGTAA GAAATTCAGG
          421 AAAGTAAATC GTTCCATTGC CATCAGCATC GACTTCATTG ATCATATCCT GCAATTCTGC
          481 TTCCGTTGGG TTTTGTCCAA GCGACCTCAT AACGGTGCCA AGTTCCTTTG TGGTGATGGT
          541 GCCGTCCCCA TCCTTGTCGA ATAATGAGAA GGCTTCTTTG AACTCTGCAA TCTGCTCTTC
          601 TGTCAGTTGG TCGGTACCGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT
          661 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC
          721 GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAANATG GTGCGCTCCT GGACGTAGCC
          781 TTCGGGCATG GCGGANTTGA ANAAGTCNTG CTGCTTCATG TGGTCNGGGT ANCGGGCGAA
          841 GCACTTCANN CCGTAGCCGA ANGTGGTCNN GANGGTGGGC CANGGCACGG GCAGCTNGNN
          901 NGTGGTGCAG ANGANCTTCN NGGTCAGCTT GCCGTANGTG GCATCGCCNN CGCCNNNNNN
          961 NGANNNGCTG AACTTGNGNN NNTTNANNNC NNCNNNCCAN CTNNNNCANG ANNGGNCNNN
        1021 NNNNNNN
        //
        Primer: pricam-M_R
        Sequence file: RDB15143_A7H9c.seq check
        >15143_15143_B1IlF_pericam-M_F_E02_05_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN GNNGCCCTTC NGCTCGATGC GGTTCNCCAG GGTGTCGCCC
           61 TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC
          121 TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG
          181 GGGTAGCGGG CGAAGCACTT CAGGCCGTAG CCGAAGGTGG TCACGAGGGT GGGCCAGGGC
          241 ACGGGCAGCT TGCCGGTGGT GCAGATGAGC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG
          301 CCCTCGCCCT CGCCGGACAC GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC
          361 AGGATGGGCA CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACACCGGT GCCACCGCTG
          421 CCACCGTCGA CCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC
          481 TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC GGACTGGAAG
          541 CTCANGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG
          601 TANTGGTCGG CGANCTGCAC GCCGCCGTCC TCGATGTTGT GNNGGANCTT GAAGTTGGNC
          661 TTGATGCCGT TCNTCTGCNN GNCNGNCNNN GNNATANACN TTGNCNNTNN NNNANNNNNN
        721 NANCCNNNNN NCNNNNNNNN NN
        //
        Primer: SV40pro_F
        Sequence file: RDB15143_A7H9d.seq check
        >D04498D1_A7H9_1-SV40pro_F_A08_02_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNANN NGTGANGNNG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
           61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
          121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
          181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
          241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
          301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
          361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
          421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
          481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
          541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
          601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
          661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
          721 AAATGGCCGC TTTTCTGGAN TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
          781 NGACATAGCG TTGGCTACNC GTGATATTGC TGAAGANCTT GGCGGCGAAT GGGCTGANCG
          841 CTTNCTCGNG CTTTACNGNA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
          901 TNNNNNCGAN TTCNTCNGAN CGGNNNNNNG GGNNTCNAAN TNANNNANNN AGCNNNNCCN
        961 NNNCNNNNCN NNNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.
        reference Fukano, T., Fast dual-excitation ratiometry with light-emitting diodes and high-speed liquid crystal shutters. Biochem. Biophys. Res. Commun. 3340 (1): 250-255 (2006). PMID 16360639.
        reference Fukano, T., Simultaneous dual-excitation ratiometry using orthogonal linear polarized lights. Biochem. Biophys. Res. Commun. 317 (1): 77-83 (2004). PMID 15047150.

        2022.02.09

        GNP_filter3_RDBDEP_html_220112.pl