RDB15235 - dKeima570/pRSETB

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

dKeima570/pRSETB (#RDB15235)

Vector backbone	Plasmid
Depositor\|Developer	Miyawaki, Atsushi \|

Sequence (partial)	RDB15235pta01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15235	dKeima570/pRSETB	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The dKeima570/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15235).

Reference section:

Kogure, T., Karasawa, S., Araki, T., Saito, K., Kinjo, M., Miyawaki, A., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15235_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724) Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB15235_A7H9a.seq
>15235_B0L4F_1_lacIpro-B_F_A03_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNCGN NNCGNNTCNG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC 61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGGATCTCG ATCCCGCGAA 121 ATTAATACGA CTCACTATAG GGAGACCACA ACGGTTTCCC TCTAGAAATA ATTTTGTTTA 181 ACTTTAAGAA GGAGATATAC ATATGCGGGG TTCTCATCAT CATCATCATC ATGGTATGGC 241 TAGCATGACT GGTGGACAGC AAATGGGTCG GGATCTGTAC GACGATGACG ATAAGGATCC 301 GACCATGGTG AGTGTGATCG CTAAACAAAT GACCTACAAG GTTTATATGT CAGGCACGGT 361 CAATGGACAC TACTTTGAGG TCGAAGGCGA TGGAAAAGGA AAGCCTTACG AGGGAGAGCA 421 GACAGTAAAG CTCACTGTCA CCAAGGGTGG ACCTCTGCCA TTTGCTTGGG ATATATTATC 481 ACCACTGATG TGTTACGGAA GCATACCATT CACCAAGTAC CCTGAAGACA TCCCTGATTA 541 TGTAAAGCAG TCATTCCCTG AGGGATATAC ATGGGAGAGG ACCATGAACT TTGAAGATGG 601 TGCAGTGTGT ACTGTCAGCA ATGATTCCAG CATCCAAGGC AACTGTTTCA TCTACAATGT 661 CAAAATCTCT GGTACGAACT TTCCTCCCAA TGGACCTGTT ATGCAGAAGA AGACACAGGG 721 CTGGGAACCC AGCACTGAGC GTCTCTTTGC ACGAGATGGA ATGCTGATAG GAAACGATTA 781 TATGGCTCTG AAGTTGGAAG GANGTGGTCA CTATTTGTGT GAATTTAAAT CTACTTACAA 841 GGCAAAGAAG CCTGTGANGA TGCCANGGTA TCACTATATT GACCGCNAAC TGGNTGTNNC 901 CAGTCACNAC AGGGATTACN CATCTGTTGA GCAGTGTGAA ATAGCCNTTG CACGCCACTC 961 TTTGCTCGGN NGANTACCTT NCNGANTTAA ATNNANGNNA GAAATACCCN NNGNNNTAAA 1021 AAAANNNANA NTTTGATTTT AGCTNNNNNN NNNN //
Primer: T7ter_R1 (Pr0798) Region: insert 3' Sequence file: RDB15235_A7H9b.seq
>15235_B0L4F_1_T7ter_R1_B03_06_ABI24.ab1 1 NNNNNNNNNN NNNGNNNNNG NNNGTNANTG NTNNGCGGTG GCAGCAGCCN ACTCAGCTTC 61 CTTTCGGGCT TTGTTAGCAG CCGGATCAAG CTTCGAATTC GCGGCCGCAG GAATTTTTTT 121 TTTTTTTTTG TTTTCAAAAG TTTAATCACT GAAGGTTGTT TACACTTCTT CGTTCATACT 181 TCTCTAAGCT AAAATCAAAA TCTACGTTTT TTTACGCCAC TGGGTATTTC TGCCTTGATT 241 TAACTCCGAA AGGTAGTCAA CCGAGCAAAG AGTGGCGTGC AATGGCTATT TCACACTGCT 301 CAACAGATGT GTAATCCCTG TTGTGACTGG TTACATCCAG TTTGCGGTCA ATATAGTGAT 361 ACCCTGGCAT CCTCACAGGC TTCTTTGCCT TGTAAGTAGA TTTAAATTCA CACAAATAGT 421 GACCACCTCC TTCCAACTTC ANAGCCATAT AATCGTTTCC TATCAGCATT CCATCTCGTG 481 CAAAGAGACG CTCAGTGCTG GGTTCCCAGC CCTGTGTCTT CTTCTGCATA ACAGGTCCAT 541 TGGGAGGAAA GTTCGTACCA NAGATTTTGA CATTGTAGAT GAAACAGTTG CCTTGGATGC 601 TGGAATCATT GCTGACAGTA CACACTGCAC CATCTTCAAA GTTCATGGTC CTCTCCCATG 661 TATATCCCTC AGGGAATGAC TGCTTTACAT AATCAGGGAT GTCTTCAGGG TACTTGGTGA 721 ATGGTATGCT TCCGTAACAC ATCAGTGGTG ATAATATATC CCAAGCAAAT GGCAGAGGTC 781 CACCCTTGGT GACAGTGAGC TTTACTGTCT GCTCTCCCTC GTAANGNTTT CCTTTTCCNT 841 CGCCTTCGAC CTCAAAGTAG TGTCCATTGN CCGNGCCTGA NATATAANCC TTGTANGTCA 901 TTTGNTTAGC GATCACACTC ACCANGGTCN GANNCTTATC GNCNTCGNCN TACAGATNCC 961 NANCNNTTTG CTGNNCNNCA NNNATGCNAG CCATANCATG ANGANGANGA TGANGANAAN 1021 CCCNNNNANG NANNTNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]

Resource search

Link

Other resources in our bank

External Database

Reference sequence