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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

dKeima570/pRSETB

Catalog number RDB15235
Resource name dKeima570/pRSETB
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15235 dKeima570/pRSETB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (partial) RDB15235pta01.seq compiled by us

Original reference

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15235_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15235_A7H9a.seq check
>15235_B0L4F_1_lacIpro-B_F_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCGN NNCGNNTCNG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC
   61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGGATCTCG ATCCCGCGAA
  121 ATTAATACGA CTCACTATAG GGAGACCACA ACGGTTTCCC TCTAGAAATA ATTTTGTTTA
  181 ACTTTAAGAA GGAGATATAC ATATGCGGGG TTCTCATCAT CATCATCATC ATGGTATGGC
  241 TAGCATGACT GGTGGACAGC AAATGGGTCG GGATCTGTAC GACGATGACG ATAAGGATCC
  301 GACCATGGTG AGTGTGATCG CTAAACAAAT GACCTACAAG GTTTATATGT CAGGCACGGT
  361 CAATGGACAC TACTTTGAGG TCGAAGGCGA TGGAAAAGGA AAGCCTTACG AGGGAGAGCA
  421 GACAGTAAAG CTCACTGTCA CCAAGGGTGG ACCTCTGCCA TTTGCTTGGG ATATATTATC
  481 ACCACTGATG TGTTACGGAA GCATACCATT CACCAAGTAC CCTGAAGACA TCCCTGATTA
  541 TGTAAAGCAG TCATTCCCTG AGGGATATAC ATGGGAGAGG ACCATGAACT TTGAAGATGG
  601 TGCAGTGTGT ACTGTCAGCA ATGATTCCAG CATCCAAGGC AACTGTTTCA TCTACAATGT
  661 CAAAATCTCT GGTACGAACT TTCCTCCCAA TGGACCTGTT ATGCAGAAGA AGACACAGGG
  721 CTGGGAACCC AGCACTGAGC GTCTCTTTGC ACGAGATGGA ATGCTGATAG GAAACGATTA
  781 TATGGCTCTG AAGTTGGAAG GANGTGGTCA CTATTTGTGT GAATTTAAAT CTACTTACAA
  841 GGCAAAGAAG CCTGTGANGA TGCCANGGTA TCACTATATT GACCGCNAAC TGGNTGTNNC
  901 CAGTCACNAC AGGGATTACN CATCTGTTGA GCAGTGTGAA ATAGCCNTTG CACGCCACTC
  961 TTTGCTCGGN NGANTACCTT NCNGANTTAA ATNNANGNNA GAAATACCCN NNGNNNTAAA
1021 AAAANNNANA NTTTGATTTT AGCTNNNNNN NNNN
//
Primer: T7ter_R1
Region: insert 3'
Sequence file: RDB15235_A7H9b.seq check
>15235_B0L4F_1_T7ter_R1_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNGNNNNNG NNNGTNANTG NTNNGCGGTG GCAGCAGCCN ACTCAGCTTC
   61 CTTTCGGGCT TTGTTAGCAG CCGGATCAAG CTTCGAATTC GCGGCCGCAG GAATTTTTTT
  121 TTTTTTTTTG TTTTCAAAAG TTTAATCACT GAAGGTTGTT TACACTTCTT CGTTCATACT
  181 TCTCTAAGCT AAAATCAAAA TCTACGTTTT TTTACGCCAC TGGGTATTTC TGCCTTGATT
  241 TAACTCCGAA AGGTAGTCAA CCGAGCAAAG AGTGGCGTGC AATGGCTATT TCACACTGCT
  301 CAACAGATGT GTAATCCCTG TTGTGACTGG TTACATCCAG TTTGCGGTCA ATATAGTGAT
  361 ACCCTGGCAT CCTCACAGGC TTCTTTGCCT TGTAAGTAGA TTTAAATTCA CACAAATAGT
  421 GACCACCTCC TTCCAACTTC ANAGCCATAT AATCGTTTCC TATCAGCATT CCATCTCGTG
  481 CAAAGAGACG CTCAGTGCTG GGTTCCCAGC CCTGTGTCTT CTTCTGCATA ACAGGTCCAT
  541 TGGGAGGAAA GTTCGTACCA NAGATTTTGA CATTGTAGAT GAAACAGTTG CCTTGGATGC
  601 TGGAATCATT GCTGACAGTA CACACTGCAC CATCTTCAAA GTTCATGGTC CTCTCCCATG
  661 TATATCCCTC AGGGAATGAC TGCTTTACAT AATCAGGGAT GTCTTCAGGG TACTTGGTGA
  721 ATGGTATGCT TCCGTAACAC ATCAGTGGTG ATAATATATC CCAAGCAAAT GGCAGAGGTC
  781 CACCCTTGGT GACAGTGAGC TTTACTGTCT GCTCTCCCTC GTAANGNTTT CCTTTTCCNT
  841 CGCCTTCGAC CTCAAAGTAG TGTCCATTGN CCGNGCCTGA NATATAANCC TTGTANGTCA
  901 TTTGNTTAGC GATCACACTC ACCANGGTCN GANNCTTATC GNCNTCGNCN TACAGATNCC
  961 NANCNNTTTG CTGNNCNNCA NNNATGCNAG CCATANCATG ANGANGANGA TGANGANAAN
1021 CCCNNNNANG NANNTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

2021.08.13

GNP_filter3_RDBDEP_html_210618.pl