RDB15459 - mCherry-hCdt1(1/100)Cy(-)/pcDNA3

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内)

[open/close]

提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

How to cite this biological resource

Tips

QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15459	mCherry-hCdt1(1/100)Cy(-)/pcDNA3	DNA solution

Featured content	Fucci-related materials (en) (English text)
Featured content	Fucci-related materials (ja) (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

original	Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]
user_report	Knoblochova, L., CHK1-CDC25A-CDK1 regulate cell cycle progression and protect genome integrity in early mouse embryos. EMBO Rep. 24 (10): e56530 (2023). PMID 37694680. [link to RRC of NBRP]

mCherry-hCdt1(1/100)Cy(-)/pcDNA3

Distribution information

How to cite this biological resource

Tips

QC test results

References

Primer: CMV_Forward () Sequence file: RDB15459_A7Jva.seq
>D05018C1-2_A7Jv_CMV-Forward_G03_21_ABI24.ab1 1 TGTCACTTCC GGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT 61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC 121 GACTCACTAT AGGGAGACCC AAGCTTGGTA CCGAGCTCGG ATCCACTAGT AACGGCCGCC 181 AGTGTGCTGG AATTCGCCAC CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC 241 AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG 301 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG 361 ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC 421 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC 481 GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC 541 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC 601 TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG 661 CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG 721 GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG 781 CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC 841 ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG 901 CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGA TGGAGCAGCG CCGCGTCACC 961 GACTTCTTCG CGCGCCGCCG CCCCGGGCCC CCCCGCATCG CGCCGCCCAA AGCTGGGCCT 1021 GCCGCACCCC CAGCCCCGCA GGGCCGCACT CGCGCCCCGG GTCTCGCCTA CAGTGCCAGC 1081 GGCAGGCGCG ACTCGGCTCG GCTCCGACTG ATCTCTGACG TCGCATCACG TTCAGCAATC 1141 GGACTCCGTA CGATCGA //
Primer: BGH_rev2 () Sequence file: RDB15459_A7Jvb.seq
>D05018C1-2_A7Jv_BGH_rev2_H03_24_ABI24.ab1 1 CATCAGGGCT AGGAGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA 61 GTCGAGGCTG ATCAGCGAGC TCTAGCATTT AGGTGACACT ATAGAATAGG GCCCTCTAGA 121 TTATTTCTTT ATCTTCTGGC CCGGAGAAGG GCAGGCTGGG ATGTCTGGGG CCTCGGGGGT 181 ACTGGGGCTG GAAACCTCGT CCACCGACAG CCGCGCGGCC GCGCGGGCCG GTGGCCTGGC 241 CTGGTCGCGT CCGGGGGCGG CGGGCGGGCG GGCGCGCTTG CGGCTGCCAC TGGTAGCGGA 301 GGCCGGGGCG CGGAGTGCGG GCCTGGCGGG GCTGGGGGTG CGGCAGGCCA GCTTGGGCGG 361 CGCGATGCGG GGGGGCCCGG GGCGGCGGCG CGCGAAGAAG TCGGTGACGC GGCGCTGCTC 421 CATCTCGAGC GGCCGCCAGT GTGATGGATA TCCCTTGTAC AGCTCGTCCA TGCCGCCGGT 481 GGAGTGGCGG CCCTCGGCGC GTTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA 541 GGTGATGTCC AACTTGATGT TGACGTTGTA GGCGCCGGGC AGCTGCACGG GCTTCTTGGC 601 CTTGTAGGTG GTCTTGACCT CAGCGTCGTA GTGGCCGCCG TCCTTCAGCT TCAGCCTCTG 661 CTTGATCTCG CCCTTCAGGG CGCCGTCCTC GGGGTACATC CGCTCGGAGG AGGCCTCCCA 721 GCCCATGGTC TTCTTCTGCA TTACGGGGCC GTCGGAGGGG AAGTTGGTGC CGCGCAGCTT 781 CACCTTGTAG ATGAACTCGC CGTCCTGCAG GGAGGAGTCC TGGGTCACGG TCACCACGCC 841 GCCGTCCTCG AAGTTCATCA CGCGCTCCCA CTTGAAGCCC TCGGGGAAGG ACAGCTTCAA 901 GTAGTCGGGG ATGTCGGCGG GGTGCTTCAC GTAGGCCTTG GAGCCGTACA TGAACTGAGG 961 GGACAGGATG TCCCAGGCGA AAGGGCAGGG GGCCACCCCT TGGTCACCTT TCAGCTTTGG 1021 CGGGTCTGGG TTGCCCCTCG TTAGGGCGGT CTTCGCCCCT TCGGCCCTTC GATCTCGAAA 1081 CTCGTGTCGA TCACGGAAGT CCGTCAATGA TGCACCTTGA AGCGGCA //
Primer: SV40pro_F () Sequence file: RDB15459_A7Jvc.seq
>D05018C1_2_A7Jv_SV40pro_F_A07_01_ABI24.ab1 1 ACTGTCCTGG ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT 121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC 181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG 301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG 421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG 481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG 601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC 661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG 721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC 781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC 901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC 961 CCAACCCTGC CCATCACGAG ATTTCGATTC CCACCCGCCC GCCCTTTCTA TGAAGGTTGG 1021 GCTTTCGGAA TCGTTTTCCG GGACGCCGGG CTGGATGATC CTCAGCCGCC GGGGAATCTC 1081 AATGCTTGGG AGTTCTCGTG ACCTCGACTT GGTTTAATTG CCAGCTTAAT AATGGTAC //