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mCherry-hCdt1(1/100)Cy(-)/pcDNA3

Expression vector of mCherry-hCdt1(1/100)Cy(-).

Catalog number RDB15459
Resource name mCherry-hCdt1(1/100)Cy(-)/pcDNA3
Clone info. Expression vector of mCherry-hCdt1(1/100)Cy(-).
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Human CDT1 cDNA Discosoma sp. RFP cDNA
Reference of insert sequence LC171208 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Discosoma sp. RFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
          Ordering Please visit Information of Request for Distribution.[link] UCSD's MTA is also required. Please visit Additional Forms section.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          UCSD's MTA is also required. Please visit Additional Forms section.
          Distribution information (domestic users) [open/close]


          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15459 mCherry-hCdt1(1/100)Cy(-)/pcDNA3 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15459_A7Jvp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Sequence file: RDB15459_A7Jva.seq check
          >D05018C1-2_A7Jv_CMV-Forward_G03_21_ABI24.ab1
              1 TGTCACTTCC GGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
            121 GACTCACTAT AGGGAGACCC AAGCTTGGTA CCGAGCTCGG ATCCACTAGT AACGGCCGCC
            181 AGTGTGCTGG AATTCGCCAC CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC
            241 AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG
            301 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG
            361 ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC
            421 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC
            481 GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC
            541 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC
            601 TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG
            661 CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG
            721 GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG
            781 CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC
            841 ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG
            901 CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGA TGGAGCAGCG CCGCGTCACC
            961 GACTTCTTCG CGCGCCGCCG CCCCGGGCCC CCCCGCATCG CGCCGCCCAA AGCTGGGCCT
           1021 GCCGCACCCC CAGCCCCGCA GGGCCGCACT CGCGCCCCGG GTCTCGCCTA CAGTGCCAGC
           1081 GGCAGGCGCG ACTCGGCTCG GCTCCGACTG ATCTCTGACG TCGCATCACG TTCAGCAATC
          1141 GGACTCCGTA CGATCGA
          //
          Primer: BGH_rev2
          Sequence file: RDB15459_A7Jvb.seq check
          >D05018C1-2_A7Jv_BGH_rev2_H03_24_ABI24.ab1
              1 CATCAGGGCT AGGAGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA
             61 GTCGAGGCTG ATCAGCGAGC TCTAGCATTT AGGTGACACT ATAGAATAGG GCCCTCTAGA
            121 TTATTTCTTT ATCTTCTGGC CCGGAGAAGG GCAGGCTGGG ATGTCTGGGG CCTCGGGGGT
            181 ACTGGGGCTG GAAACCTCGT CCACCGACAG CCGCGCGGCC GCGCGGGCCG GTGGCCTGGC
            241 CTGGTCGCGT CCGGGGGCGG CGGGCGGGCG GGCGCGCTTG CGGCTGCCAC TGGTAGCGGA
            301 GGCCGGGGCG CGGAGTGCGG GCCTGGCGGG GCTGGGGGTG CGGCAGGCCA GCTTGGGCGG
            361 CGCGATGCGG GGGGGCCCGG GGCGGCGGCG CGCGAAGAAG TCGGTGACGC GGCGCTGCTC
            421 CATCTCGAGC GGCCGCCAGT GTGATGGATA TCCCTTGTAC AGCTCGTCCA TGCCGCCGGT
            481 GGAGTGGCGG CCCTCGGCGC GTTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA
            541 GGTGATGTCC AACTTGATGT TGACGTTGTA GGCGCCGGGC AGCTGCACGG GCTTCTTGGC
            601 CTTGTAGGTG GTCTTGACCT CAGCGTCGTA GTGGCCGCCG TCCTTCAGCT TCAGCCTCTG
            661 CTTGATCTCG CCCTTCAGGG CGCCGTCCTC GGGGTACATC CGCTCGGAGG AGGCCTCCCA
            721 GCCCATGGTC TTCTTCTGCA TTACGGGGCC GTCGGAGGGG AAGTTGGTGC CGCGCAGCTT
            781 CACCTTGTAG ATGAACTCGC CGTCCTGCAG GGAGGAGTCC TGGGTCACGG TCACCACGCC
            841 GCCGTCCTCG AAGTTCATCA CGCGCTCCCA CTTGAAGCCC TCGGGGAAGG ACAGCTTCAA
            901 GTAGTCGGGG ATGTCGGCGG GGTGCTTCAC GTAGGCCTTG GAGCCGTACA TGAACTGAGG
            961 GGACAGGATG TCCCAGGCGA AAGGGCAGGG GGCCACCCCT TGGTCACCTT TCAGCTTTGG
           1021 CGGGTCTGGG TTGCCCCTCG TTAGGGCGGT CTTCGCCCCT TCGGCCCTTC GATCTCGAAA
          1081 CTCGTGTCGA TCACGGAAGT CCGTCAATGA TGCACCTTGA AGCGGCA
          //
          Primer: SV40pro_F
          Sequence file: RDB15459_A7Jvc.seq check
          >D05018C1_2_A7Jv_SV40pro_F_A07_01_ABI24.ab1
              1 ACTGTCCTGG ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
             61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
            121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
            181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
            241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
            301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
            361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
            421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
            481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
            541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
            601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
            661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
            721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
            781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
            841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
            901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC
            961 CCAACCCTGC CCATCACGAG ATTTCGATTC CCACCCGCCC GCCCTTTCTA TGAAGGTTGG
           1021 GCTTTCGGAA TCGTTTTCCG GGACGCCGGG CTGGATGATC CTCAGCCGCC GGGGAATCTC
          1081 AATGCTTGGG AGTTCTCGTG ACCTCGACTT GGTTTAATTG CCAGCTTAAT AATGGTAC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

          2021.11.01

          GNP_filter3_RDBDEP_html_211029.pl