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mCherry-hCdt1(1/100)Cy(-)/pcDNA3

Expression vector of mCherry-hCdt1(1/100)Cy(-).

Catalog number RDB15459
Resource name mCherry-hCdt1(1/100)Cy(-)/pcDNA3
Clone info. Expression vector of mCherry-hCdt1(1/100)Cy(-).
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Human CDT1 cDNA Discosoma sp. RFP cDNA
Reference of nucleotide sequence LC171208 (DDBJ accession number)
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15459 mCherry-hCdt1(1/100)Cy(-)/pcDNA3 DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15459_A7Jvp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB15459_A7Jva.seq
>D05018C1-2_A7Jv_CMV-Forward_G03_21_ABI24.ab1
    1 TGTCACTTCC GGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTTGGTA CCGAGCTCGG ATCCACTAGT AACGGCCGCC
  181 AGTGTGCTGG AATTCGCCAC CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC
  241 AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG
  301 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG
  361 ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC
  421 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC
  481 GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC
  541 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC
  601 TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG
  661 CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG
  721 GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG
  781 CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC
  841 ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG
  901 CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGA TGGAGCAGCG CCGCGTCACC
  961 GACTTCTTCG CGCGCCGCCG CCCCGGGCCC CCCCGCATCG CGCCGCCCAA AGCTGGGCCT
 1021 GCCGCACCCC CAGCCCCGCA GGGCCGCACT CGCGCCCCGG GTCTCGCCTA CAGTGCCAGC
 1081 GGCAGGCGCG ACTCGGCTCG GCTCCGACTG ATCTCTGACG TCGCATCACG TTCAGCAATC
1141 GGACTCCGTA CGATCGA
//
Primer: BGH_rev2 RDB15459_A7Jvb.seq
>D05018C1-2_A7Jv_BGH_rev2_H03_24_ABI24.ab1
    1 CATCAGGGCT AGGAGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGAGC TCTAGCATTT AGGTGACACT ATAGAATAGG GCCCTCTAGA
  121 TTATTTCTTT ATCTTCTGGC CCGGAGAAGG GCAGGCTGGG ATGTCTGGGG CCTCGGGGGT
  181 ACTGGGGCTG GAAACCTCGT CCACCGACAG CCGCGCGGCC GCGCGGGCCG GTGGCCTGGC
  241 CTGGTCGCGT CCGGGGGCGG CGGGCGGGCG GGCGCGCTTG CGGCTGCCAC TGGTAGCGGA
  301 GGCCGGGGCG CGGAGTGCGG GCCTGGCGGG GCTGGGGGTG CGGCAGGCCA GCTTGGGCGG
  361 CGCGATGCGG GGGGGCCCGG GGCGGCGGCG CGCGAAGAAG TCGGTGACGC GGCGCTGCTC
  421 CATCTCGAGC GGCCGCCAGT GTGATGGATA TCCCTTGTAC AGCTCGTCCA TGCCGCCGGT
  481 GGAGTGGCGG CCCTCGGCGC GTTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA
  541 GGTGATGTCC AACTTGATGT TGACGTTGTA GGCGCCGGGC AGCTGCACGG GCTTCTTGGC
  601 CTTGTAGGTG GTCTTGACCT CAGCGTCGTA GTGGCCGCCG TCCTTCAGCT TCAGCCTCTG
  661 CTTGATCTCG CCCTTCAGGG CGCCGTCCTC GGGGTACATC CGCTCGGAGG AGGCCTCCCA
  721 GCCCATGGTC TTCTTCTGCA TTACGGGGCC GTCGGAGGGG AAGTTGGTGC CGCGCAGCTT
  781 CACCTTGTAG ATGAACTCGC CGTCCTGCAG GGAGGAGTCC TGGGTCACGG TCACCACGCC
  841 GCCGTCCTCG AAGTTCATCA CGCGCTCCCA CTTGAAGCCC TCGGGGAAGG ACAGCTTCAA
  901 GTAGTCGGGG ATGTCGGCGG GGTGCTTCAC GTAGGCCTTG GAGCCGTACA TGAACTGAGG
  961 GGACAGGATG TCCCAGGCGA AAGGGCAGGG GGCCACCCCT TGGTCACCTT TCAGCTTTGG
 1021 CGGGTCTGGG TTGCCCCTCG TTAGGGCGGT CTTCGCCCCT TCGGCCCTTC GATCTCGAAA
1081 CTCGTGTCGA TCACGGAAGT CCGTCAATGA TGCACCTTGA AGCGGCA
//
Primer: SV40pro_F RDB15459_A7Jvc.seq
>D05018C1_2_A7Jv_SV40pro_F_A07_01_ABI24.ab1
    1 ACTGTCCTGG ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC
  961 CCAACCCTGC CCATCACGAG ATTTCGATTC CCACCCGCCC GCCCTTTCTA TGAAGGTTGG
 1021 GCTTTCGGAA TCGTTTTCCG GGACGCCGGG CTGGATGATC CTCAGCCGCC GGGGAATCTC
1081 AATGCTTGGG AGTTCTCGTG ACCTCGACTT GGTTTAATTG CCAGCTTAAT AATGGTAC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

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References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2017.12.04

GNP_filter3_RDBDEP_html_171106.pl