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AmCyan-hGem(1/110)/pCSII-EF

Lentivirus vector plasmid of AmCyan-hGeminin(1/110). EF-1 alpha promoter.

Catalog number RDB15274
Resource name AmCyan-hGem(1/110)/pCSII-EF
Clone info. Lentivirus vector plasmid of AmCyan-hGeminin(1/110). EF-1 alpha promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells).
Depositor|Developer Miyawaki, Atsushi |
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External Database

            Reference sequence
              
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
            Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
            3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
            4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
            Ordering Please visit Ordering instruction.[link] 
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            This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
            Remarks Remember that you will be working with samples containing infectious virus.
            This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
            Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
            3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
            4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
            付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            提供条件は英文のまま記載してください。
            Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15274 AmCyan-hGem(1/110)/pCSII-EF DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Nishimura, K., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fucci-related materials (en) (English text)
            Featured content Fucci-related materials (ja) (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15274_A7H3p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F
            Region: EF-1 alpha pro,insert 5'(AmCyan-hGem 1/110)
            Sequence file: RDB15274_A7H3a.seq check
            >D04498I5_2_A7H3_EF1a-C_F_H01_22_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTTTG ANTTTGGNNC TTGGTTCNTT CTCNNGCCTC AGACAGTGGT
               61 TCAAAGTTTT TTTCTTCCNT TTCAGGTGTC GTGAACACGC TACCGGTCTC GAGAATTCGC
              121 CACCATGGCC CTGTCCAACA AGTTCATCGG CGACGACATG AAGATGACCT ACCACATGGA
              181 CGGCTGCGTG AACGGCCACT ACTTCACCGT GAAGGGCGAG GGCAGCGGCA AGCCCTACGA
              241 GGGCACCCAG ACCTCCACCT TCAAGGTGAC CATGGCCAAC GGCGGCCCCC TGGCCTTCTC
              301 CTTCGACATC CTGTCCACCG TGTTCATGTA CGGCAACCGC TGCTTCACCG CCTACCCCAC
              361 CAGCATGCCC GACTACTTCA AGCAGGCCTT CCCCGACGGC ATGTCCTACG AGAGAACCTT
              421 CACCTACGAG GACGGCGGCG TGGCCACCGC CAGCTGGGAG ATCAGCCTGA AGGGCAACTG
              481 CTTCGAGCAC AAGTCCACCT TCCACGGCGT GAACTTCCCC GCCGACGGCC CCGTGATGGC
              541 CAAGAAGACC ACCGGCTGGG ACCCCTCCTT CGAGAAGATG ACCGTGTGCG ACGGCATCTT
              601 GAAGGGCGAC GTGACCGCCT TCCTGATGCT GCAGGGCGGC GGCAACTACA GATGCCAGTT
              661 CCACACCTCC TACNAGACCN AGAAGCCCGT GACCATGCCC CCCAACCNCG TGGTGGAGCA
              721 CCGCATCGCC AGANCCGACC TGGACAAGGG CGGCAACAGC GTGCAGCTGA CCGAGCACGC
              781 CGNGGCCCNC ATCACCTCCG TGGTGNCCTT CGGANATCCN TCACACTGGC GGCCGCTCGA
              841 GATGAATCCC AGTATGAANC AGAAACNAGA ANAAATCAAA GAGAATATAA ANNATAGTTC
              901 TGNCCCNAGA ANANNTCTGA ANANGANNCN GCNNTCNGCN TCTGGNTCTC NNGNNNNAAG
              961 ANAAAANGAN CTGTNNNNNN NTNGNNNNAA NNNANNCNTC NNNNNGNCNN CTTNNNNTCN
            1021 NNNNNTTNNN NNNNNNGGNN NNNNNNNN
            //
            Primer: PRE_rev
            Region: PRE,insert 3'(AmCyan-hGem 1/110)
            Sequence file: RDB15274_A7H3b.seq check
            >D04498I5-2_A7H3_PRE_rev_H02_23_ABI24.ab1
                1 NNNNNNNNNN NNNNCGNAAN NGGANCNNCN TAGTTAAGAA TACCAGTCAA TCTTTCACAA
               61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
              121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT
              181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT
              241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC
              301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG
              361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT
              421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC
              481 CGAAGGGCAC CACGGAGGTG ATGTGGGCCA CGGCGTGCTC GGTCAGCTGC ACGCTGTTGC
              541 CGCCCTTGTC CAGGTCGGTT CTGGCGATGC GGTGCTCCAC CACGTGGTTG GGGGGCATGG
              601 TCACGGGCTT CTTGGTCTTG TAGGAGGTGT GGAACTGGCA TCTGTAGTTG CCGCCGCCCT
              661 GCAGCATCAG GAAGGCGGTC ACGTCGCCCT TCAAGATGCC GTCGCACACG GTCATCTTCT
              721 CGAANGANGG GTCCCAGCCG GTGGTCTTCT TGGCCATCAC GGGGCCGTCN GCGGGGAAGT
              781 TCACGCCGTG NAANGTGGAN TTGTGCTCNA AGCANTTGNC CTTCNNGNTG ATCTCCNAGC
              841 TGGCGGTGGN CNCGCCGCCN TCNTCNNNNG TGAAGGNTCT CTCGTANGAN NTGNCNNCNG
              901 GGANNNNNTG CTTGAANTAN TCGGGNNTGC TGGNGGGGNA NGCGGNGAAN NANCGGNTGN
            961 NNNACNTGNN CNNNNNGNNN NNNANGNCNN ANNNNNNNNN N
            //
            Primer: CMV_Forward
            Region: CMV pro,5' LTR,HIV-1 psi
            Sequence file: RDB15274_A7H3c.seq check
            >D04498I5-2_A7H3_CMV-Forward_H03_24_ABI24.ab1
                1 NNNNNNNNNN NNCNNNTTGN NGCNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
               61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
              121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
              181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
              241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
              301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
              361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
              421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
              481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
              541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
              601 CTGGGACAGC TACNACCATC CCTTCNGACA GGATCAGAAG AACTTAGATC ATTATATAAT
              661 ACAGTAGCAA CCNTCTATTG TGTGCATCNA AGGATAGAGA TAAAAGACAC CNANGAAGCT
              721 TTAGACNAGA TAGAGGAAGA NCNAAACNAA AGTAANACCN CCGCACAGCN NNNNCCGCTG
              781 ATCTTCNNAN CNNNNNGAGG ANANNNGANG GANNNTNNGN NAAGNGAATT NTATNAANNN
              841 NANGTANTAA AAATTGNNCN NTTNNNNTAN CNNCNNNCNN NNNAAGANNA NANTNNNGCN
            901 NANNNNAAAA ANANCNNNNN NNNNN
            //
            Primer: RRE_F1
            Region: RRE
            Sequence file: RDB15274_A7H3d.seq check
            >15274_15274_B1DqF_RRE_F1_C01_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNC NNNNNNNNTG CTGANGGCTA TTGANNGCGC AACAGCATCT
               61 GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG
              121 ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC
              181 CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA
              241 CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT
              301 AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA
              361 ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT
              421 CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT
              481 GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG
              541 GGGANCCGAC AGGCCCGAAG GAATAGAAGA AGAANGTGGA GAGAGAGANA GANACAGANC
              601 CNTTCNATTA GTGNANGGAT CTACNAATGG NNGTANTCNT NCNNNNTTTN AAAGAAANGG
            661 GGGNNNNNGG GGGNNACNNN GNNNGGNNNN NNNNNN
            //
            Primer: cPPT/CTS-E_R
            Region: cPPT_CTS
            Sequence file: RDB15274_A7H3e.seq check
            >15274_15274_B1DqF_cPPT_CTS-E_R_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNTNNNNNN NNNCTTTAGT TTGTATGTCT GTTGCTATTN NNGTCTACTA
               61 TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA
              121 CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT
              181 TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC
              241 GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT
              301 CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT
              361 GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA
              421 TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG
              481 TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA
              541 TTACTCNNAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC AGANCAACCC
              601 CNAATCCCCA GGAGCTGTTG ATCCTTTANG TANCTTTCCN CAGCCAGGNN TCNTGNCTGG
              661 ANCTGCTTGA TGNCCCNGAN TGNGAGTNGC NACNNANGCN GNNGCNNCTC NNNANNCNNC
            721 ANNNANNNNN NNNNN
            //
            Primer: BGH_rev2
            Region: BGH pA,3' LTR
            Sequence file: RDB15274_A7H3f.seq check
            >D04498I5-2_A7H3_BGH_rev2_H04_22_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN GGGGGNNGGG NNNNNACNGA TGGCTGGCAA CTAGAAGGCA
               61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
              121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
              181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
              241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
              301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
              361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
              421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
              481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
              541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
              601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
              661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
              721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCNTGTTT TTCTAGGTCT
              781 CGACNGTATC GATGCNGGGA GGCGGCCCNA AGGGAGNTCC GACTCGTCTG ANGGCGAANG
              841 NNAAGACNCG GAANANNNCN CNNANNNNNN AGCNNGNCNC NGGAANGANN GNNCNCTNGA
              901 TTNAGGGNCN NANNGNACNN NNNNNANNNN NNNNCCGCGC ANAANCCNNN NGNNNNCNCN
            961 NNNANCANCC NTNGAANNNN NNTNNNNNTN CCNNNNNNNN NN
            //
            Primer: SV40pro_F
            Region: SV40pro_ori,Zeo
            Sequence file: RDB15274_A7H3g.seq check
            >D04498I5-2_A7H3_SV40pro_F_H05_23_ABI24.ab1
                1 CNNNNNNNNN NNNNNNNNNN NNNNNNGTGA GGANGNTTTT TTGGAGGNCT AGGCTTTTGC
               61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
              121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
              181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
              241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
              301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
              361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
              421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
              481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
              541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
              601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
              661 TGGAGTTCTT CGCCCACCCN AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
              721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
              781 CCNAACTCAT CNATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTNGG
              841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TNCGCTCNCN ATTCCNCACA
              901 ACATACGAGC CNGANGCNTN AAGNGNAAAG NNNGGGGNGC CTNANGAGTG AGCTAACTCA
              961 CNTTNATTGC GTTGCGCNNN CTGCCNNCTT TNCNNNNNGG NAANCNGNCG NNNNNGCNNN
            1021 NNNNANNNNA NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Nishimura, K., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789.
            reference Oki, T., A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. Sci. Rep. 4: 4012 (2014). PMID 24500246.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl