Please review the QC test results indicated by icon below as well as clone information before placing your order.

AmCyan-hGem(1/110)/pCSII-EF

Lentivirus vector plasmid of AmCyan-hGeminin(1/110). EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
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Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15274_A7H3p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(AmCyan-hGem 1/110) Sequence file: RDB15274_A7H3a.seq
>D04498I5_2_A7H3_EF1a-C_F_H01_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTTG ANTTTGGNNC TTGGTTCNTT CTCNNGCCTC AGACAGTGGT    61 TCAAAGTTTT TTTCTTCCNT TTCAGGTGTC GTGAACACGC TACCGGTCTC GAGAATTCGC   121 CACCATGGCC CTGTCCAACA AGTTCATCGG CGACGACATG AAGATGACCT ACCACATGGA   181 CGGCTGCGTG AACGGCCACT ACTTCACCGT GAAGGGCGAG GGCAGCGGCA AGCCCTACGA   241 GGGCACCCAG ACCTCCACCT TCAAGGTGAC CATGGCCAAC GGCGGCCCCC TGGCCTTCTC   301 CTTCGACATC CTGTCCACCG TGTTCATGTA CGGCAACCGC TGCTTCACCG CCTACCCCAC   361 CAGCATGCCC GACTACTTCA AGCAGGCCTT CCCCGACGGC ATGTCCTACG AGAGAACCTT   421 CACCTACGAG GACGGCGGCG TGGCCACCGC CAGCTGGGAG ATCAGCCTGA AGGGCAACTG   481 CTTCGAGCAC AAGTCCACCT TCCACGGCGT GAACTTCCCC GCCGACGGCC CCGTGATGGC   541 CAAGAAGACC ACCGGCTGGG ACCCCTCCTT CGAGAAGATG ACCGTGTGCG ACGGCATCTT   601 GAAGGGCGAC GTGACCGCCT TCCTGATGCT GCAGGGCGGC GGCAACTACA GATGCCAGTT   661 CCACACCTCC TACNAGACCN AGAAGCCCGT GACCATGCCC CCCAACCNCG TGGTGGAGCA   721 CCGCATCGCC AGANCCGACC TGGACAAGGG CGGCAACAGC GTGCAGCTGA CCGAGCACGC   781 CGNGGCCCNC ATCACCTCCG TGGTGNCCTT CGGANATCCN TCACACTGGC GGCCGCTCGA   841 GATGAATCCC AGTATGAANC AGAAACNAGA ANAAATCAAA GAGAATATAA ANNATAGTTC   901 TGNCCCNAGA ANANNTCTGA ANANGANNCN GCNNTCNGCN TCTGGNTCTC NNGNNNNAAG   961 ANAAAANGAN CTGTNNNNNN NTNGNNNNAA NNNANNCNTC NNNNNGNCNN CTTNNNNTCN 1021 NNNNNTTNNN NNNNNNGGNN NNNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,insert 3'(AmCyan-hGem 1/110) Sequence file: RDB15274_A7H3b.seq
>D04498I5-2_A7H3_PRE_rev_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNNCGNAAN NGGANCNNCN TAGTTAAGAA TACCAGTCAA TCTTTCACAA    61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT   121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT   181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT   241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC   301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG   361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT   421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC   481 CGAAGGGCAC CACGGAGGTG ATGTGGGCCA CGGCGTGCTC GGTCAGCTGC ACGCTGTTGC   541 CGCCCTTGTC CAGGTCGGTT CTGGCGATGC GGTGCTCCAC CACGTGGTTG GGGGGCATGG   601 TCACGGGCTT CTTGGTCTTG TAGGAGGTGT GGAACTGGCA TCTGTAGTTG CCGCCGCCCT   661 GCAGCATCAG GAAGGCGGTC ACGTCGCCCT TCAAGATGCC GTCGCACACG GTCATCTTCT   721 CGAANGANGG GTCCCAGCCG GTGGTCTTCT TGGCCATCAC GGGGCCGTCN GCGGGGAAGT   781 TCACGCCGTG NAANGTGGAN TTGTGCTCNA AGCANTTGNC CTTCNNGNTG ATCTCCNAGC   841 TGGCGGTGGN CNCGCCGCCN TCNTCNNNNG TGAAGGNTCT CTCGTANGAN NTGNCNNCNG   901 GGANNNNNTG CTTGAANTAN TCGGGNNTGC TGGNGGGGNA NGCGGNGAAN NANCGGNTGN 961 NNNACNTGNN CNNNNNGNNN NNNANGNCNN ANNNNNNNNN N //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1 psi Sequence file: RDB15274_A7H3c.seq
>D04498I5-2_A7H3_CMV-Forward_H03_24_ABI24.ab1     1 NNNNNNNNNN NNCNNNTTGN NGCNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT   121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA   181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA   241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA   301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG   361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT   421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG   481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG   541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA   601 CTGGGACAGC TACNACCATC CCTTCNGACA GGATCAGAAG AACTTAGATC ATTATATAAT   661 ACAGTAGCAA CCNTCTATTG TGTGCATCNA AGGATAGAGA TAAAAGACAC CNANGAAGCT   721 TTAGACNAGA TAGAGGAAGA NCNAAACNAA AGTAANACCN CCGCACAGCN NNNNCCGCTG   781 ATCTTCNNAN CNNNNNGAGG ANANNNGANG GANNNTNNGN NAAGNGAATT NTATNAANNN   841 NANGTANTAA AAATTGNNCN NTTNNNNTAN CNNCNNNCNN NNNAAGANNA NANTNNNGCN 901 NANNNNAAAA ANANCNNNNN NNNNN //
Primer: RRE_F1 (Pr0796) Region: RRE Sequence file: RDB15274_A7H3d.seq
>15274_15274_B1DqF_RRE_F1_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNC NNNNNNNNTG CTGANGGCTA TTGANNGCGC AACAGCATCT    61 GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG   121 ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC   181 CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA   241 CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT   301 AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA   361 ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT   421 CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT   481 GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG   541 GGGANCCGAC AGGCCCGAAG GAATAGAAGA AGAANGTGGA GAGAGAGANA GANACAGANC   601 CNTTCNATTA GTGNANGGAT CTACNAATGG NNGTANTCNT NCNNNNTTTN AAAGAAANGG 661 GGGNNNNNGG GGGNNACNNN GNNNGGNNNN NNNNNN //
Primer: cPPT/CTS-E_R (Pr0725) Region: cPPT_CTS Sequence file: RDB15274_A7H3e.seq
>15274_15274_B1DqF_cPPT_CTS-E_R_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNTNNNNNN NNNCTTTAGT TTGTATGTCT GTTGCTATTN NNGTCTACTA    61 TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA   121 CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT   181 TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC   241 GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT   301 CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT   361 GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA   421 TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG   481 TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA   541 TTACTCNNAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC AGANCAACCC   601 CNAATCCCCA GGAGCTGTTG ATCCTTTANG TANCTTTCCN CAGCCAGGNN TCNTGNCTGG   661 ANCTGCTTGA TGNCCCNGAN TGNGAGTNGC NACNNANGCN GNNGCNNCTC NNNANNCNNC 721 ANNNANNNNN NNNNN //
Primer: BGH_rev2 (Pr0606) Region: BGH pA,3' LTR Sequence file: RDB15274_A7H3f.seq
>D04498I5-2_A7H3_BGH_rev2_H04_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GGGGGNNGGG NNNNNACNGA TGGCTGGCAA CTAGAAGGCA    61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA   121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA   181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC   241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG   301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG   361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT   421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA   481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG   541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC   601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA   661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA   721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCNTGTTT TTCTAGGTCT   781 CGACNGTATC GATGCNGGGA GGCGGCCCNA AGGGAGNTCC GACTCGTCTG ANGGCGAANG   841 NNAAGACNCG GAANANNNCN CNNANNNNNN AGCNNGNCNC NGGAANGANN GNNCNCTNGA   901 TTNAGGGNCN NANNGNACNN NNNNNANNNN NNNNCCGCGC ANAANCCNNN NGNNNNCNCN 961 NNNANCANCC NTNGAANNNN NNTNNNNNTN CCNNNNNNNN NN //
Primer: SV40pro_F (Pr0110) Region: SV40pro_ori,Zeo Sequence file: RDB15274_A7H3g.seq
>D04498I5-2_A7H3_SV40pro_F_H05_23_ABI24.ab1     1 CNNNNNNNNN NNNNNNNNNN NNNNNNGTGA GGANGNTTTT TTGGAGGNCT AGGCTTTTGC    61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT   121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG   181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT   241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG   301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA   361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG   421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT   481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG   541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG   601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC   661 TGGAGTTCTT CGCCCACCCN AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA   721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT   781 CCNAACTCAT CNATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTNGG   841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TNCGCTCNCN ATTCCNCACA   901 ACATACGAGC CNGANGCNTN AAGNGNAAAG NNNGGGGNGC CTNANGAGTG AGCTAACTCA   961 CNTTNATTGC GTTGCGCNNN CTGCCNNCTT TNCNNNNNGG NAANCNGNCG NNNNNGCNNN 1021 NNNNANNNNA NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.12.12

GNP_filter3_RDBDEP_html_231212.pl