Resource data sheet
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AmCyan-hGem(1/110)/pCSII-EF

Lentivirus vector plasmid of AmCyan-hGeminin(1/110). EF-1 alpha promoter.

Catalog number RDB15274
Resource name AmCyan-hGem(1/110)/pCSII-EF
Clone info. Lentivirus vector plasmid of AmCyan-hGeminin(1/110). EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells).
Depositor|Developer Miyawaki, Atsushi |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering Please visit Ordering instruction.[link] 
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Exclusive MTA (for use for not-for-profit academic purpose)[Link]
Remarks Remember that you will be working with samples containing infectious virus.
The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs).
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15274 AmCyan-hGem(1/110)/pCSII-EF Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Nishimura, K., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fucci-related materials (en) (English text)
Featured content Fucci-related materials (ja) (Japanese text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Nishimura, K., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789.
reference Oki, T., A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. Sci. Rep. 4: 4012 (2014). PMID 24500246.

2020.12.24

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