RDB15274 - AmCyan-hGem(1/110)/pCSII-EF

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AmCyan-hGem(1/110)/pCSII-EF (#RDB15274)

Lentivirus vector plasmid of AmCyan-hGeminin(1/110). EF-1 alpha promoter.

Clone info.	Lentivirus vector plasmid of AmCyan-hGeminin(1/110). EF-1 alpha promoter.
Vector backbone	Plasmid
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

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Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Ordering instruction.[link]
Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. MTAに書く付加的使用条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15274	AmCyan-hGem(1/110)/pCSII-EF	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The AmCyan-hGem(1/110)/pCSII-EF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15274).

Reference section:

Nishimura, K., Oki, T., Kitaura, J., Kuninaka, S., Saya, H., Sakaue-Sawano, A., Miyawaki, A., Kitamura, T., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15274_A7H3p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(AmCyan-hGem 1/110) Sequence file: RDB15274_A7H3a.seq
>D04498I5_2_A7H3_EF1a-C_F_H01_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNTTTG ANTTTGGNNC TTGGTTCNTT CTCNNGCCTC AGACAGTGGT 61 TCAAAGTTTT TTTCTTCCNT TTCAGGTGTC GTGAACACGC TACCGGTCTC GAGAATTCGC 121 CACCATGGCC CTGTCCAACA AGTTCATCGG CGACGACATG AAGATGACCT ACCACATGGA 181 CGGCTGCGTG AACGGCCACT ACTTCACCGT GAAGGGCGAG GGCAGCGGCA AGCCCTACGA 241 GGGCACCCAG ACCTCCACCT TCAAGGTGAC CATGGCCAAC GGCGGCCCCC TGGCCTTCTC 301 CTTCGACATC CTGTCCACCG TGTTCATGTA CGGCAACCGC TGCTTCACCG CCTACCCCAC 361 CAGCATGCCC GACTACTTCA AGCAGGCCTT CCCCGACGGC ATGTCCTACG AGAGAACCTT 421 CACCTACGAG GACGGCGGCG TGGCCACCGC CAGCTGGGAG ATCAGCCTGA AGGGCAACTG 481 CTTCGAGCAC AAGTCCACCT TCCACGGCGT GAACTTCCCC GCCGACGGCC CCGTGATGGC 541 CAAGAAGACC ACCGGCTGGG ACCCCTCCTT CGAGAAGATG ACCGTGTGCG ACGGCATCTT 601 GAAGGGCGAC GTGACCGCCT TCCTGATGCT GCAGGGCGGC GGCAACTACA GATGCCAGTT 661 CCACACCTCC TACNAGACCN AGAAGCCCGT GACCATGCCC CCCAACCNCG TGGTGGAGCA 721 CCGCATCGCC AGANCCGACC TGGACAAGGG CGGCAACAGC GTGCAGCTGA CCGAGCACGC 781 CGNGGCCCNC ATCACCTCCG TGGTGNCCTT CGGANATCCN TCACACTGGC GGCCGCTCGA 841 GATGAATCCC AGTATGAANC AGAAACNAGA ANAAATCAAA GAGAATATAA ANNATAGTTC 901 TGNCCCNAGA ANANNTCTGA ANANGANNCN GCNNTCNGCN TCTGGNTCTC NNGNNNNAAG 961 ANAAAANGAN CTGTNNNNNN NTNGNNNNAA NNNANNCNTC NNNNNGNCNN CTTNNNNTCN 1021 NNNNNTTNNN NNNNNNGGNN NNNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,insert 3'(AmCyan-hGem 1/110) Sequence file: RDB15274_A7H3b.seq
>D04498I5-2_A7H3_PRE_rev_H02_23_ABI24.ab1 1 NNNNNNNNNN NNNNCGNAAN NGGANCNNCN TAGTTAAGAA TACCAGTCAA TCTTTCACAA 61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT 121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT 181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT 241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC 301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG 361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT 421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC 481 CGAAGGGCAC CACGGAGGTG ATGTGGGCCA CGGCGTGCTC GGTCAGCTGC ACGCTGTTGC 541 CGCCCTTGTC CAGGTCGGTT CTGGCGATGC GGTGCTCCAC CACGTGGTTG GGGGGCATGG 601 TCACGGGCTT CTTGGTCTTG TAGGAGGTGT GGAACTGGCA TCTGTAGTTG CCGCCGCCCT 661 GCAGCATCAG GAAGGCGGTC ACGTCGCCCT TCAAGATGCC GTCGCACACG GTCATCTTCT 721 CGAANGANGG GTCCCAGCCG GTGGTCTTCT TGGCCATCAC GGGGCCGTCN GCGGGGAAGT 781 TCACGCCGTG NAANGTGGAN TTGTGCTCNA AGCANTTGNC CTTCNNGNTG ATCTCCNAGC 841 TGGCGGTGGN CNCGCCGCCN TCNTCNNNNG TGAAGGNTCT CTCGTANGAN NTGNCNNCNG 901 GGANNNNNTG CTTGAANTAN TCGGGNNTGC TGGNGGGGNA NGCGGNGAAN NANCGGNTGN 961 NNNACNTGNN CNNNNNGNNN NNNANGNCNN ANNNNNNNNN N //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1 psi Sequence file: RDB15274_A7H3c.seq
>D04498I5-2_A7H3_CMV-Forward_H03_24_ABI24.ab1 1 NNNNNNNNNN NNCNNNTTGN NGCNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT 121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA 181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA 241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA 301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG 361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT 421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG 481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG 541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA 601 CTGGGACAGC TACNACCATC CCTTCNGACA GGATCAGAAG AACTTAGATC ATTATATAAT 661 ACAGTAGCAA CCNTCTATTG TGTGCATCNA AGGATAGAGA TAAAAGACAC CNANGAAGCT 721 TTAGACNAGA TAGAGGAAGA NCNAAACNAA AGTAANACCN CCGCACAGCN NNNNCCGCTG 781 ATCTTCNNAN CNNNNNGAGG ANANNNGANG GANNNTNNGN NAAGNGAATT NTATNAANNN 841 NANGTANTAA AAATTGNNCN NTTNNNNTAN CNNCNNNCNN NNNAAGANNA NANTNNNGCN 901 NANNNNAAAA ANANCNNNNN NNNNN //
Primer: RRE_F1 (Pr0796) Region: RRE Sequence file: RDB15274_A7H3d.seq
>15274_15274_B1DqF_RRE_F1_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNC NNNNNNNNTG CTGANGGCTA TTGANNGCGC AACAGCATCT 61 GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG 121 ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC 181 CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA 241 CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT 301 AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA 361 ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT 421 CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT 481 GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG 541 GGGANCCGAC AGGCCCGAAG GAATAGAAGA AGAANGTGGA GAGAGAGANA GANACAGANC 601 CNTTCNATTA GTGNANGGAT CTACNAATGG NNGTANTCNT NCNNNNTTTN AAAGAAANGG 661 GGGNNNNNGG GGGNNACNNN GNNNGGNNNN NNNNNN //
Primer: cPPT/CTS-E_R (Pr0725) Region: cPPT_CTS Sequence file: RDB15274_A7H3e.seq
>15274_15274_B1DqF_cPPT_CTS-E_R_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNTNNNNNN NNNCTTTAGT TTGTATGTCT GTTGCTATTN NNGTCTACTA 61 TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA 121 CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT 181 TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC 241 GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT 301 CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT 361 GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA 421 TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG 481 TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA 541 TTACTCNNAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC AGANCAACCC 601 CNAATCCCCA GGAGCTGTTG ATCCTTTANG TANCTTTCCN CAGCCAGGNN TCNTGNCTGG 661 ANCTGCTTGA TGNCCCNGAN TGNGAGTNGC NACNNANGCN GNNGCNNCTC NNNANNCNNC 721 ANNNANNNNN NNNNN //
Primer: BGH_rev2 (Pr0606) Region: BGH pA,3' LTR Sequence file: RDB15274_A7H3f.seq
>D04498I5-2_A7H3_BGH_rev2_H04_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GGGGGNNGGG NNNNNACNGA TGGCTGGCAA CTAGAAGGCA 61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA 121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA 181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC 241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG 301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG 361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT 421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA 481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG 541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC 601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA 661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA 721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCNTGTTT TTCTAGGTCT 781 CGACNGTATC GATGCNGGGA GGCGGCCCNA AGGGAGNTCC GACTCGTCTG ANGGCGAANG 841 NNAAGACNCG GAANANNNCN CNNANNNNNN AGCNNGNCNC NGGAANGANN GNNCNCTNGA 901 TTNAGGGNCN NANNGNACNN NNNNNANNNN NNNNCCGCGC ANAANCCNNN NGNNNNCNCN 961 NNNANCANCC NTNGAANNNN NNTNNNNNTN CCNNNNNNNN NN //
Primer: SV40pro_F (Pr0110) Region: SV40pro_ori,Zeo Sequence file: RDB15274_A7H3g.seq
>D04498I5-2_A7H3_SV40pro_F_H05_23_ABI24.ab1 1 CNNNNNNNNN NNNNNNNNNN NNNNNNGTGA GGANGNTTTT TTGGAGGNCT AGGCTTTTGC 61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT 121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG 181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT 241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG 301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA 361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG 421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT 481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG 541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 661 TGGAGTTCTT CGCCCACCCN AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 781 CCNAACTCAT CNATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTNGG 841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TNCGCTCNCN ATTCCNCACA 901 ACATACGAGC CNGANGCNTN AAGNGNAAAG NNNGGGGNGC CTNANGAGTG AGCTAACTCA 961 CNTTNATTGC GTTGCGCNNN CTGCCNNCTT TNCNNNNNGG NAANCNGNCG NNNNNGCNNN 1021 NNNNANNNNA NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nishimura, K., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789. [link to RRC of NBRP]
reference	Oki, T., A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. Sci. Rep. 4: 4012 (2014). PMID 24500246.

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