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AmCyan-hGem(1/110)/pCSII-EF (#RDB15274)

Lentivirus vector plasmid of AmCyan-hGeminin(1/110). EF-1 alpha promoter.

Clone info. Lentivirus vector plasmid of AmCyan-hGeminin(1/110). EF-1 alpha promoter.
Vector backbone Plasmid
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

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In order to receive this biological resource, please request the Exclusive MTA to us.
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Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
MTAに書く付加的使用条件:
レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15274 AmCyan-hGem(1/110)/pCSII-EF DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The AmCyan-hGem(1/110)/pCSII-EF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15274).

Reference section:

Nishimura, K., Oki, T., Kitaura, J., Kuninaka, S., Saya, H., Sakaue-Sawano, A., Miyawaki, A., Kitamura, T., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15274_A7H3p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro,insert 5'(AmCyan-hGem 1/110)
Sequence file: RDB15274_A7H3a.seq check
>D04498I5_2_A7H3_EF1a-C_F_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTTG ANTTTGGNNC TTGGTTCNTT CTCNNGCCTC AGACAGTGGT
   61 TCAAAGTTTT TTTCTTCCNT TTCAGGTGTC GTGAACACGC TACCGGTCTC GAGAATTCGC
  121 CACCATGGCC CTGTCCAACA AGTTCATCGG CGACGACATG AAGATGACCT ACCACATGGA
  181 CGGCTGCGTG AACGGCCACT ACTTCACCGT GAAGGGCGAG GGCAGCGGCA AGCCCTACGA
  241 GGGCACCCAG ACCTCCACCT TCAAGGTGAC CATGGCCAAC GGCGGCCCCC TGGCCTTCTC
  301 CTTCGACATC CTGTCCACCG TGTTCATGTA CGGCAACCGC TGCTTCACCG CCTACCCCAC
  361 CAGCATGCCC GACTACTTCA AGCAGGCCTT CCCCGACGGC ATGTCCTACG AGAGAACCTT
  421 CACCTACGAG GACGGCGGCG TGGCCACCGC CAGCTGGGAG ATCAGCCTGA AGGGCAACTG
  481 CTTCGAGCAC AAGTCCACCT TCCACGGCGT GAACTTCCCC GCCGACGGCC CCGTGATGGC
  541 CAAGAAGACC ACCGGCTGGG ACCCCTCCTT CGAGAAGATG ACCGTGTGCG ACGGCATCTT
  601 GAAGGGCGAC GTGACCGCCT TCCTGATGCT GCAGGGCGGC GGCAACTACA GATGCCAGTT
  661 CCACACCTCC TACNAGACCN AGAAGCCCGT GACCATGCCC CCCAACCNCG TGGTGGAGCA
  721 CCGCATCGCC AGANCCGACC TGGACAAGGG CGGCAACAGC GTGCAGCTGA CCGAGCACGC
  781 CGNGGCCCNC ATCACCTCCG TGGTGNCCTT CGGANATCCN TCACACTGGC GGCCGCTCGA
  841 GATGAATCCC AGTATGAANC AGAAACNAGA ANAAATCAAA GAGAATATAA ANNATAGTTC
  901 TGNCCCNAGA ANANNTCTGA ANANGANNCN GCNNTCNGCN TCTGGNTCTC NNGNNNNAAG
  961 ANAAAANGAN CTGTNNNNNN NTNGNNNNAA NNNANNCNTC NNNNNGNCNN CTTNNNNTCN
1021 NNNNNTTNNN NNNNNNGGNN NNNNNNNN
//
Primer: PRE_rev (Pr0250)
Region: PRE,insert 3'(AmCyan-hGem 1/110)
Sequence file: RDB15274_A7H3b.seq check
>D04498I5-2_A7H3_PRE_rev_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNCGNAAN NGGANCNNCN TAGTTAAGAA TACCAGTCAA TCTTTCACAA
   61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
  121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT
  181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT
  241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC
  301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG
  361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT
  421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC
  481 CGAAGGGCAC CACGGAGGTG ATGTGGGCCA CGGCGTGCTC GGTCAGCTGC ACGCTGTTGC
  541 CGCCCTTGTC CAGGTCGGTT CTGGCGATGC GGTGCTCCAC CACGTGGTTG GGGGGCATGG
  601 TCACGGGCTT CTTGGTCTTG TAGGAGGTGT GGAACTGGCA TCTGTAGTTG CCGCCGCCCT
  661 GCAGCATCAG GAAGGCGGTC ACGTCGCCCT TCAAGATGCC GTCGCACACG GTCATCTTCT
  721 CGAANGANGG GTCCCAGCCG GTGGTCTTCT TGGCCATCAC GGGGCCGTCN GCGGGGAAGT
  781 TCACGCCGTG NAANGTGGAN TTGTGCTCNA AGCANTTGNC CTTCNNGNTG ATCTCCNAGC
  841 TGGCGGTGGN CNCGCCGCCN TCNTCNNNNG TGAAGGNTCT CTCGTANGAN NTGNCNNCNG
  901 GGANNNNNTG CTTGAANTAN TCGGGNNTGC TGGNGGGGNA NGCGGNGAAN NANCGGNTGN
961 NNNACNTGNN CNNNNNGNNN NNNANGNCNN ANNNNNNNNN N
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,5' LTR,HIV-1 psi
Sequence file: RDB15274_A7H3c.seq check
>D04498I5-2_A7H3_CMV-Forward_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNCNNNTTGN NGCNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
  121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
  181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
  241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
  301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
  361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
  421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
  481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
  541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
  601 CTGGGACAGC TACNACCATC CCTTCNGACA GGATCAGAAG AACTTAGATC ATTATATAAT
  661 ACAGTAGCAA CCNTCTATTG TGTGCATCNA AGGATAGAGA TAAAAGACAC CNANGAAGCT
  721 TTAGACNAGA TAGAGGAAGA NCNAAACNAA AGTAANACCN CCGCACAGCN NNNNCCGCTG
  781 ATCTTCNNAN CNNNNNGAGG ANANNNGANG GANNNTNNGN NAAGNGAATT NTATNAANNN
  841 NANGTANTAA AAATTGNNCN NTTNNNNTAN CNNCNNNCNN NNNAAGANNA NANTNNNGCN
901 NANNNNAAAA ANANCNNNNN NNNNN
//
Primer: RRE_F1 (Pr0796)
Region: RRE
Sequence file: RDB15274_A7H3d.seq check
>15274_15274_B1DqF_RRE_F1_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC NNNNNNNNTG CTGANGGCTA TTGANNGCGC AACAGCATCT
   61 GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG
  121 ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC
  181 CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA
  241 CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT
  301 AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA
  361 ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT
  421 CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT
  481 GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG
  541 GGGANCCGAC AGGCCCGAAG GAATAGAAGA AGAANGTGGA GAGAGAGANA GANACAGANC
  601 CNTTCNATTA GTGNANGGAT CTACNAATGG NNGTANTCNT NCNNNNTTTN AAAGAAANGG
661 GGGNNNNNGG GGGNNACNNN GNNNGGNNNN NNNNNN
//
Primer: cPPT/CTS-E_R (Pr0725)
Region: cPPT_CTS
Sequence file: RDB15274_A7H3e.seq check
>15274_15274_B1DqF_cPPT_CTS-E_R_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNTNNNNNN NNNCTTTAGT TTGTATGTCT GTTGCTATTN NNGTCTACTA
   61 TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA
  121 CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT
  181 TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC
  241 GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT
  301 CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT
  361 GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA
  421 TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG
  481 TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA
  541 TTACTCNNAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC AGANCAACCC
  601 CNAATCCCCA GGAGCTGTTG ATCCTTTANG TANCTTTCCN CAGCCAGGNN TCNTGNCTGG
  661 ANCTGCTTGA TGNCCCNGAN TGNGAGTNGC NACNNANGCN GNNGCNNCTC NNNANNCNNC
721 ANNNANNNNN NNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: BGH pA,3' LTR
Sequence file: RDB15274_A7H3f.seq check
>D04498I5-2_A7H3_BGH_rev2_H04_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GGGGGNNGGG NNNNNACNGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
  121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
  181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
  241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
  301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
  361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
  421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
  481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
  541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
  601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
  661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
  721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCNTGTTT TTCTAGGTCT
  781 CGACNGTATC GATGCNGGGA GGCGGCCCNA AGGGAGNTCC GACTCGTCTG ANGGCGAANG
  841 NNAAGACNCG GAANANNNCN CNNANNNNNN AGCNNGNCNC NGGAANGANN GNNCNCTNGA
  901 TTNAGGGNCN NANNGNACNN NNNNNANNNN NNNNCCGCGC ANAANCCNNN NGNNNNCNCN
961 NNNANCANCC NTNGAANNNN NNTNNNNNTN CCNNNNNNNN NN
//
Primer: SV40pro_F (Pr0110)
Region: SV40pro_ori,Zeo
Sequence file: RDB15274_A7H3g.seq check
>D04498I5-2_A7H3_SV40pro_F_H05_23_ABI24.ab1
    1 CNNNNNNNNN NNNNNNNNNN NNNNNNGTGA GGANGNTTTT TTGGAGGNCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
  121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
  181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
  241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
  301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
  361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
  421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
  481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
  541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
  601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
  661 TGGAGTTCTT CGCCCACCCN AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
  781 CCNAACTCAT CNATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTNGG
  841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TNCGCTCNCN ATTCCNCACA
  901 ACATACGAGC CNGANGCNTN AAGNGNAAAG NNNGGGGNGC CTNANGAGTG AGCTAACTCA
  961 CNTTNATTGC GTTGCGCNNN CTGCCNNCTT TNCNNNNNGG NAANCNGNCG NNNNNGCNNN
1021 NNNNANNNNA NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nishimura, K., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789. [link to RRC of NBRP]
reference Oki, T., A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. Sci. Rep. 4: 4012 (2014). PMID 24500246.