Resource data sheet
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mCherry-hGeminin(1/60) / pCSII-EF

Lentivirus vector plasmid of S/G2/M-R(NC).

Catalog number RDB15270
Resource name mCherry-hGeminin(1/60) / pCSII-EF
Clone info. Lentivirus vector plasmid of S/G2/M-R(NC). Fluorescence was detected in both the nucleus and the cytoplasm.
For the composition, refer Fig.1 of Sakaue-Sawano, A., Chem. Biol. 15 (12): 1243-1248 (2008). Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name human Geminin cDNA Discosoma sp. RFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank human GMNN (NCBI Gene 51053) |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering Please visit Ordering instruction.[link] UCSD's MTA is also required. Please visit Additional Forms section.
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks Remember that you will be working with samples containing infectious virus.
UCSD's MTA is also required. Please visit Additional Forms section.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15270 mCherry-hGeminin(1/60) / pCSII-EF Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fucci-related materials (en) (English text)
Featured content Fucci-related materials (ja) (Japanese text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.
reference Cadart, C., Size control in mammalian cells involves modulation of both growth rate and cell cycle duration. Nat. Commun. 9 (1): 3275 (2018). PMID 30115907.
reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.

2020.12.24

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