Resource data sheet
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mKO2-hCdt1(30/120) / pCSII-EF

Lentivirus vector construct of Fucci-G1 phase probe. Component of Fucci cell cycle probe.

Catalog number RDB15267
Resource name mKO2-hCdt1(30/120) / pCSII-EF
Alternative name FUCCI-Red
Clone info. Lentiviral expression vector of Fucci-G1 phase probe. One of the component of Fucci cell cycle probe. Fluorescence was detected in the nucleus.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (Plasmid)
Size of vector backbone 9.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Human CDT1 cDNA Fungia concinna OFP cDNA
Originator Miyawaki, Atsushi |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A. et al. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell, 132 (3): 487-498.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Ordering instruction. 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Additional form)) Dr. Miyawaki's Approval
Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15267 mKO2-hCdt1(30/120) / pCSII-EF DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Fucci-related materials (en) (English text)
Featured content Fucci-related materials (ja) (Japanese text)

References

original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15267_A7H3p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15267_A7H3a.seq
>D04498H7-1_A7H3_EF1a-C_F_A01_01_ABI24.ab1
    1 TTCTTGTGGA ATTTGGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCCAGAC
   61 AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA ACACGCTACC GGTCTCGAGA
  121 ATTCGCCACC ATGGTGAGTG TGATTAAACC AGAGATGAAG ATGAGGTACT ACATGGACGG
  181 CTCCGTCAAT GGGCATGAGT TCACAATTGA AGGTGAAGGC ACAGGCAGAC CTTACGAGGG
  241 ACATCAAGAG ATGACACTAC GCGTCACAAT GGCCGAGGGC GGGCCAATGC CTTTCGCGTT
  301 TGACTTAGTG TCACACGTGT TCTGTTACGG CCACAGAGTA TTTACTAAAT ATCCAGAAGA
  361 GATACCAGAC TATTTCAAAC AAGCATTTCC TGAAGGCCTG TCATGGGAAA GGTCGTTGGA
  421 GTTCGAAGAT GGTGGGTCCG CTTCAGTCAG TGCGCATATA AGCCTTAGAG GAAACACCTT
  481 CTACCACAAA TCCAAATTTA CTGGGGTTAA CTTTCCTGCC GATGGTCCTA TCATGCAAAA
  541 CCAAAGTGTT GATTGGGAGC CATCAACCGA GAAAATTACT GCCAGCGACG GAGTTCTGAA
  601 GGGTGATGTT ACGATGTACC TAAAACTTGA AGGAGGCGGC AATCACAAAT GCCAAATGAA
  661 GACTACTTAC AAGGCGGCAA AAGAGATTCT TGAAATGCCA GGAGACCATT ACATCGGCCA
  721 TCGCCTCGTC AGGAAAACCG AAGGCAACAT TACTGAGCAG GTAGAAGATG CAGTAGCTCA
  781 TTACTCAATG TTGCCTTCCC AGGGCGGATA TCCATCACAC TGGCGGCCGC TCGAGCCCAG
  841 CCCCGCCAGG CCCGCACTCC GCGCCCCGGC CTCCGCTACC AGTGGCAGCC GCAAGCGCGC
  901 CCGCCCGCCC GCCGCCCCCG GACGCGACCA GGGCCAGGCC ACCGGCCCGC AGGAGACTGC
  961 GGCTGTCGGT GGACGAGGTT TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCC
 1021 TGCCCTTCTC CGGGCCAGAA GATAAAGAAT CCACCCCCGG GCAGCAAGGT CAGCCCGCCC
 1081 CACCTGACAT CCCGCGGCAG GACCAGGACA CCATCTATCT AGAGGTTAAC ATCGAAGGAT
 1141 CAGCTAATCG ATAATTCACC CTCTGGATTA CAAAATTTGG TGAAAGAAAT TGTGACACCG
1201 TGGGTAT
//
Primer: PRE_rev RDB15267_A7H3b.seq
>D04498H7-1_A7H3_PRE_rev_C09_09_ABI24.ab1
    1 TCGGTTCAAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT
   61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTAGA
  121 TGGTGTCCTG GTCCTGCGCG GATGTCAGGT GGGGCGGCTG ACCTGCTGCC GGGGTGGATT
  181 TCTTTATCTT CTGGCCCGGA GAAGGGCAGG CTGGGATGTC TGGGGCCTCG GGGGTACTGG
  241 GGCTGGAAAC CTCGTCCACC GACAGCCGCA GTCTCCTGCG GGCCGGTGGC CTGGCCTGGT
  301 CGCGTCCGGG GGCGGCGGGC GGGCGGGCGC GCTTGCGGCT GCCACTGGTA GCGGAGGCCG
  361 GGGCGCGGAG TGCGGGCCTG GCGGGGCTGG GCTCGAGCGG CCGCCAGTGT GATGGATATC
  421 CGCCCTGGGA AGGCAACATT GAGTAATGAG CTACTGCATC TTCTACCTGC TCAGTAATGT
  481 TGCCTTCGGT TTTCCTGACG AGGCGATGGC CGATGTAATG GTCTCCTGGC ATTTCAAGAA
  541 TCTCTTTTGC CGCCTTGTAA GTAGTCTTCA TTTGGCATTT GTGATTGCCG CCTCCTTCAA
  601 GTTTTAGGTA CATCGTAACA TCACCCTTCA GAACTCCGTC GCTGGCAGTA ATTTTCTCGG
  661 TTGATGGCTC CCAATCAACA CTTTGGTTTT GCATGATAGG ACCATCGGCA GGAAAGTTAA
  721 CCCCAGTAAA TTTGGATTTG TGGTAGAAGG TGTTTCCTCT AAGGCTTATA TGCGCACTGA
  781 CTGAAGCGGA CCCACCATCT TCGAACTCCA ACGACCTTTC CCATGACAGG CCTTCAGGAA
  841 ATGCTTGTTT GAAATAGTCT GGTATCTCTT CTGGATATTT AGTAAATACT CTGTGGCCGT
  901 AACAGAACAC GTGTGACACT AAGTCAAACG CGAAAGGCAT TGGCCCGCCC TCGGGCCATT
  961 GTGACGCGTA GTGTCATCTC TTGATGTCCC TCGTAAAGGT CTGCCTGTGC CCTTCACCTT
 1021 TCAATTGTGA ACTCATGCCC ATTGACGGAG CCGTCCATGT AGTACCTCCA TCCTCATCTC
 1081 TGGGTTTAAT CACACTTCAC CATTGCTTGA CCGACTTCCT CGAGACCGGT AGCGGTGGTT
1141 TCCACCGAAA CACACCCCCC
//
Primer: CMV-Forward RDB15267_A7H3c.seq
>D04498H7-1_A7H3_CMV-Forward_D09_12_ABI24.ab1
    1 TGTTACACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
  121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
  181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
  241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
  301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
  361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
  421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
  481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
  541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
  601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
  661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
  721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG
  781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT
  841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG
  901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA
  961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTATTGTCT
 1021 GGGTATAGTG CAGCAGCAGA ACAATTTGCT GAGGGCTATT TGAGGCGCAA CCAGCCATCT
 1081 GGTGGCACTC ACAGTCTGGG CAATCAGGCA GCTCAGCCAA GGAATCTGGG CTGGTGGGAA
1141 AGAAATAACC TTAG
//
Primer: BGH_rev2 RDB15267_A7H3d.seq
>D04498H7-1_A7H3_BGH_rev2_A04_01_ABI24.ab1
    1 CTCTCACGGC CAGAGGCACG GGGGAGGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
  121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
  181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
  241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
  301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
  361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
  421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
  481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
  541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
  601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
  661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
  721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC
  781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC
  841 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG TCCGCTGGGA
  901 TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG
  961 GCGAGCAGCC ATGGAAAGGA CGTCAGCTTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC
 1021 AACAGCCCGA GCCCCTGTCC AGCAGCGGGC AAGCAGCGGC GATGAGTTCC GCCGTTGGCC
 1081 AATAGGGAGG GGGAAGGCGA AGGTTCCGAG GGACCTGACA GGTGGTGCCA TGCCTAACCA
1141 GATGGGGGGG TGTGCGTCCA C
//
Primer: SV40pro_F RDB15267_A7H3e.seq
>D04498H7-1_A7H3_SV40pro_F_A05_02_ABI24.ab1
    1 CCTCCGGTTC CTGTGGCATT CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA
  121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT
  181 GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA
  241 GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT
  301 GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA
  361 CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT
  421 CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC
  481 GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA
  541 GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT
  601 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT
  661 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
  721 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT
  781 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT
  841 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
  901 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT
  961 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
 1021 GCATTAATGA AATCGCCCAA CGCGCGGGGA GAAGGCGGTT TGCGTATGGG GCGCTCTTCC
 1081 GCTTCTCGCT CACTGACTCC GCTGCGCTCG TCGTCGGCTG CCGGCCGAGC CGTATTCAGG
1141 CTCCACCTCA AAGGGCCGGT AATTAACCGG GCT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078.

2018.10.09

GNP_filter3_RDBDEP_html_180823.pl