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mKO2-hCdt1(30/120) / pCSII-EF

Lentivirus vector plasmid of Fucci-G1 phase probe. Component of Fucci cell cycle probe.

Catalog number RDB15267
Resource name mKO2-hCdt1(30/120) / pCSII-EF
Alternative name FUCCI-Red
Clone info. Lentivirus vector plasmid of Fucci-G1 phase probe. One of the component of Fucci cell cycle probe. Fluorescence was detected in the nucleus.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (Plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Fungia concinna OFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Fungia concinna OFP

          Reference sequence
            
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano, A. et al. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell, 132 (3): 487-498.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
          Ordering Please visit Ordering instruction.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano, A. et al. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell, 132 (3): 487-498.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 【追加提供依頼書】受入れ確認書(書式J)
          このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          提供条件は英文のまま記載してください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15267 mKO2-hCdt1(30/120) / pCSII-EF DNA solution

          Ordering instruction of plasmids [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15267_A7H3p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Region: EF-1a pro,insert5'
          Sequence file: RDB15267_A7H3a.seq check
          >D04498H7-1_A7H3_EF1a-C_F_A01_01_ABI24.ab1
              1 TTCTTGTGGA ATTTGGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCCAGAC
             61 AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA ACACGCTACC GGTCTCGAGA
            121 ATTCGCCACC ATGGTGAGTG TGATTAAACC AGAGATGAAG ATGAGGTACT ACATGGACGG
            181 CTCCGTCAAT GGGCATGAGT TCACAATTGA AGGTGAAGGC ACAGGCAGAC CTTACGAGGG
            241 ACATCAAGAG ATGACACTAC GCGTCACAAT GGCCGAGGGC GGGCCAATGC CTTTCGCGTT
            301 TGACTTAGTG TCACACGTGT TCTGTTACGG CCACAGAGTA TTTACTAAAT ATCCAGAAGA
            361 GATACCAGAC TATTTCAAAC AAGCATTTCC TGAAGGCCTG TCATGGGAAA GGTCGTTGGA
            421 GTTCGAAGAT GGTGGGTCCG CTTCAGTCAG TGCGCATATA AGCCTTAGAG GAAACACCTT
            481 CTACCACAAA TCCAAATTTA CTGGGGTTAA CTTTCCTGCC GATGGTCCTA TCATGCAAAA
            541 CCAAAGTGTT GATTGGGAGC CATCAACCGA GAAAATTACT GCCAGCGACG GAGTTCTGAA
            601 GGGTGATGTT ACGATGTACC TAAAACTTGA AGGAGGCGGC AATCACAAAT GCCAAATGAA
            661 GACTACTTAC AAGGCGGCAA AAGAGATTCT TGAAATGCCA GGAGACCATT ACATCGGCCA
            721 TCGCCTCGTC AGGAAAACCG AAGGCAACAT TACTGAGCAG GTAGAAGATG CAGTAGCTCA
            781 TTACTCAATG TTGCCTTCCC AGGGCGGATA TCCATCACAC TGGCGGCCGC TCGAGCCCAG
            841 CCCCGCCAGG CCCGCACTCC GCGCCCCGGC CTCCGCTACC AGTGGCAGCC GCAAGCGCGC
            901 CCGCCCGCCC GCCGCCCCCG GACGCGACCA GGGCCAGGCC ACCGGCCCGC AGGAGACTGC
            961 GGCTGTCGGT GGACGAGGTT TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCC
           1021 TGCCCTTCTC CGGGCCAGAA GATAAAGAAT CCACCCCCGG GCAGCAAGGT CAGCCCGCCC
           1081 CACCTGACAT CCCGCGGCAG GACCAGGACA CCATCTATCT AGAGGTTAAC ATCGAAGGAT
           1141 CAGCTAATCG ATAATTCACC CTCTGGATTA CAAAATTTGG TGAAAGAAAT TGTGACACCG
          1201 TGGGTAT
          //
          Primer: PRE_rev
          Region: PRE,insert3'
          Sequence file: RDB15267_A7H3b.seq check
          >D04498H7-1_A7H3_PRE_rev_C09_09_ABI24.ab1
              1 TCGGTTCAAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT
             61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTAGA
            121 TGGTGTCCTG GTCCTGCGCG GATGTCAGGT GGGGCGGCTG ACCTGCTGCC GGGGTGGATT
            181 TCTTTATCTT CTGGCCCGGA GAAGGGCAGG CTGGGATGTC TGGGGCCTCG GGGGTACTGG
            241 GGCTGGAAAC CTCGTCCACC GACAGCCGCA GTCTCCTGCG GGCCGGTGGC CTGGCCTGGT
            301 CGCGTCCGGG GGCGGCGGGC GGGCGGGCGC GCTTGCGGCT GCCACTGGTA GCGGAGGCCG
            361 GGGCGCGGAG TGCGGGCCTG GCGGGGCTGG GCTCGAGCGG CCGCCAGTGT GATGGATATC
            421 CGCCCTGGGA AGGCAACATT GAGTAATGAG CTACTGCATC TTCTACCTGC TCAGTAATGT
            481 TGCCTTCGGT TTTCCTGACG AGGCGATGGC CGATGTAATG GTCTCCTGGC ATTTCAAGAA
            541 TCTCTTTTGC CGCCTTGTAA GTAGTCTTCA TTTGGCATTT GTGATTGCCG CCTCCTTCAA
            601 GTTTTAGGTA CATCGTAACA TCACCCTTCA GAACTCCGTC GCTGGCAGTA ATTTTCTCGG
            661 TTGATGGCTC CCAATCAACA CTTTGGTTTT GCATGATAGG ACCATCGGCA GGAAAGTTAA
            721 CCCCAGTAAA TTTGGATTTG TGGTAGAAGG TGTTTCCTCT AAGGCTTATA TGCGCACTGA
            781 CTGAAGCGGA CCCACCATCT TCGAACTCCA ACGACCTTTC CCATGACAGG CCTTCAGGAA
            841 ATGCTTGTTT GAAATAGTCT GGTATCTCTT CTGGATATTT AGTAAATACT CTGTGGCCGT
            901 AACAGAACAC GTGTGACACT AAGTCAAACG CGAAAGGCAT TGGCCCGCCC TCGGGCCATT
            961 GTGACGCGTA GTGTCATCTC TTGATGTCCC TCGTAAAGGT CTGCCTGTGC CCTTCACCTT
           1021 TCAATTGTGA ACTCATGCCC ATTGACGGAG CCGTCCATGT AGTACCTCCA TCCTCATCTC
           1081 TGGGTTTAAT CACACTTCAC CATTGCTTGA CCGACTTCCT CGAGACCGGT AGCGGTGGTT
          1141 TCCACCGAAA CACACCCCCC
          //
          Primer: CMV_Forward
          Region: CMV pro,5'LTR,HIV-1 psi,RRE
          Sequence file: RDB15267_A7H3c.seq check
          >D04498H7-1_A7H3_CMV-Forward_D09_12_ABI24.ab1
              1 TGTTACACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
            121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
            181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
            241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
            301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
            361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
            421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
            481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
            541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
            601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
            661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
            721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG
            781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT
            841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG
            901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA
            961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTATTGTCT
           1021 GGGTATAGTG CAGCAGCAGA ACAATTTGCT GAGGGCTATT TGAGGCGCAA CCAGCCATCT
           1081 GGTGGCACTC ACAGTCTGGG CAATCAGGCA GCTCAGCCAA GGAATCTGGG CTGGTGGGAA
          1141 AGAAATAACC TTAG
          //
          Primer: cPPT_CTS-E_R
          Region: cPPT_CTS,RRE
          Sequence file: RDB15267_A7H3f.seq check
          >15268_15268_B0B4F_cPPT_CTS-E_R_F02_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCT TTAGTTTGTA TGTCTGTTGC TATTATGTCT
             61 ACTATTCTTT CCCCTGCACT GTACCCCCCA ATCCCCCCTT TTCTTTTAAA ATTGTGGATG
            121 AATACTGCCA TTTGTAGATC CGTTCACTAA TCGAATGGAT CTGTCTCTGT CTCTCTCTCC
            181 ACCTTCTTCT TCTATTCCTT CGGGCCTGTC GGGTCCCCTC GGGGTTGGGA GGTGGGTCTG
            241 AAACGATAAT GGTGAATATC CCTGCCTAAC TCTATTCACT ATAGAAAGTA CAGCAAAAAC
            301 TATTCTTAAA CCTACCAAGC CTCCTACTAT CATTATGAAT AATTTTATAT ACCACAGCCA
            361 ATTTGTTATG TTAAACCAAT TCCACAAACT TGCCCATTTA TCTAATTCCA ATAATTCTTG
            421 TTCATTCTTT TCTTGCTGGT TTTGCGATTC TTCAATTAAG GAGTGTATTA AGCTTGTGTA
            481 ATTGTTAATT TCTCTGTCCC ACTCCATCCA GGTCGTGTGA TTCCAAATCT GTTCCAGAGA
            541 TTTATTACTC CAACTAGCAT TCCAAGGCAC AGCAGTGGTG CAAATGAGTT TTCCAGAGCA
            601 ACCCCAAATC CCCAGGAGCT GTTGATCCTT TAGGTATCTT TCCACAGCCA GGATTCTTGC
            661 CTGGAGCTGC TTGATGCCCC AGACTGTGAG TTGCAACAGA TGCTGTTGCG CCTCAATAGC
            721 CCTCAGCAAA TTGTTCTGCT GCTGCACTAT ANCAGACAAT AATTGTCTGG CCTGTACCGT
            781 CNGCGTCATT GANGCTGCGC CCATAGTGCT TCCTGCTGCT CCCNAGAACN CAAGGANCAA
            841 AGCTNCTATT NCNACNGCTC TTTTTTTCTC TCTGCNNNAC TCNNNTCTTN GCCTTNNNGG
            901 GNGCNACNNC TNANGGTNCN NTTTTTACTA CTTTATATTT ATATNANNCA CTTCTCNNAN
          961 NNNNCCNNNA NATNNNCNNN NNNNNNN
          //
          Primer: BGH_rev2
          Region: bGHp(A),3'LTR,PRE
          Sequence file: RDB15267_A7H3d.seq check
          >D04498H7-1_A7H3_BGH_rev2_A04_01_ABI24.ab1
              1 CTCTCACGGC CAGAGGCACG GGGGAGGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC
             61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
            121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
            181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
            241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
            301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
            361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
            421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
            481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
            541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
            601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
            661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
            721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC
            781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC
            841 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG TCCGCTGGGA
            901 TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG
            961 GCGAGCAGCC ATGGAAAGGA CGTCAGCTTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC
           1021 AACAGCCCGA GCCCCTGTCC AGCAGCGGGC AAGCAGCGGC GATGAGTTCC GCCGTTGGCC
           1081 AATAGGGAGG GGGAAGGCGA AGGTTCCGAG GGACCTGACA GGTGGTGCCA TGCCTAACCA
          1141 GATGGGGGGG TGTGCGTCCA C
          //
          Primer: SV40pro_F
          Region: SV40 pro_ori,Zeo,SV40p(A)
          Sequence file: RDB15267_A7H3e.seq check
          >D04498H7-1_A7H3_SV40pro_F_A05_02_ABI24.ab1
              1 CCTCCGGTTC CTGTGGCATT CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
             61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA
            121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT
            181 GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA
            241 GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT
            301 GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA
            361 CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT
            421 CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC
            481 GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA
            541 GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT
            601 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT
            661 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
            721 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT
            781 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT
            841 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
            901 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT
            961 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
           1021 GCATTAATGA AATCGCCCAA CGCGCGGGGA GAAGGCGGTT TGCGTATGGG GCGCTCTTCC
           1081 GCTTCTCGCT CACTGACTCC GCTGCGCTCG TCGTCGGCTG CCGGCCGAGC CGTATTCAGG
          1141 CTCCACCTCA AAGGGCCGGT AATTAACCGG GCT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078.
          reference Möller, J., Process-induced cell cycle oscillations in CHO cultures: Online monitoring and model-based investigation. Biotechnol. Bioeng. 116 (11): 2931-2943 (2019). PMID 31342512.
          reference Takahashi, H., Significance of Polypyrimidine Tract-Binding Protein 1 Expression in Colorectal Cancer. Mol. Cancer. Ther. 14 (7): 1705-1716 (2015). PMID 25904505.
          reference Beaumont, K.A., Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids. J. Vis. Exp. (106):e53486 (2015). PMID 26779761.
          reference Kagawa, Y., Cell Cycle-Dependent Rho GTPase Activity Dynamically Regulates Cancer Cell Motility and Invasion In Vivo. PLoS One, 8 (12): e83629 (2013). PMID 24386239.
          user_report Tanaka, A., Reversible expansion of pancreatic islet progenitors derived from human induced pluripotent stem cells. Genes Cells 25 (5): 302-311 (2020). PMID 32065490.
          user_report Onozato, Y., Radiosensitivity of quiescent and proliferating cells grown as multicellular tumor spheroids. Cancer Sci. 108 (4): 704-712 (2017). PMID 28135769.
          user_report Sereti, K.I., Methods to study the proliferation and differentiation of cardiac side population (CSP) cells. Methods Mol. Biol. 1036: 95-106 (2013). PMID 23807790.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl