Sequencing and PCR primers for primer information.
Lentivirus vector construct of Fucci-G1 phase probe. Component of Fucci cell cycle probe.
| Catalog number | RDB15267 |
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| Resource name | mKO2-hCdt1(30/120) / pCSII-EF |
| Alternative name | FUCCI-Red |
| Clone info. | Lentiviral expression vector of Fucci-G1 phase probe. One of the component of Fucci cell cycle probe. Fluorescence was detected in the nucleus. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
| Vector backbone | CSII-EF-MCS (Plasmid) |
| Size of vector backbone | 9.1 kb |
| Selectable markers | Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells). |
| Gene/insert name | Human CDT1 cDNA Fungia concinna OFP cDNA |
| Originator | Miyawaki, Atsushi | |
| Terms and conditions for distribution | 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A. et al. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell, 132 (3): 487-498. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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| Ordering | Please visit Ordering instruction. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
| Remarks | Remember that you will be working with samples containing infectious virus. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15267 | mKO2-hCdt1(30/120) / pCSII-EF | DNA solution |
Electronic file
Featured content
| Featured content | Fucci-related materials (en) (English text) |
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| Featured content | Fucci-related materials (ja) (Japanese text) |
References
| original | Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15267_A7H3p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: EF1a-C_F | RDB15267_A7H3a.seq |
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>D04498H7-1_A7H3_EF1a-C_F_A01_01_ABI24.ab1 1 TTCTTGTGGA ATTTGGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCCAGAC 61 AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA ACACGCTACC GGTCTCGAGA 121 ATTCGCCACC ATGGTGAGTG TGATTAAACC AGAGATGAAG ATGAGGTACT ACATGGACGG 181 CTCCGTCAAT GGGCATGAGT TCACAATTGA AGGTGAAGGC ACAGGCAGAC CTTACGAGGG 241 ACATCAAGAG ATGACACTAC GCGTCACAAT GGCCGAGGGC GGGCCAATGC CTTTCGCGTT 301 TGACTTAGTG TCACACGTGT TCTGTTACGG CCACAGAGTA TTTACTAAAT ATCCAGAAGA 361 GATACCAGAC TATTTCAAAC AAGCATTTCC TGAAGGCCTG TCATGGGAAA GGTCGTTGGA 421 GTTCGAAGAT GGTGGGTCCG CTTCAGTCAG TGCGCATATA AGCCTTAGAG GAAACACCTT 481 CTACCACAAA TCCAAATTTA CTGGGGTTAA CTTTCCTGCC GATGGTCCTA TCATGCAAAA 541 CCAAAGTGTT GATTGGGAGC CATCAACCGA GAAAATTACT GCCAGCGACG GAGTTCTGAA 601 GGGTGATGTT ACGATGTACC TAAAACTTGA AGGAGGCGGC AATCACAAAT GCCAAATGAA 661 GACTACTTAC AAGGCGGCAA AAGAGATTCT TGAAATGCCA GGAGACCATT ACATCGGCCA 721 TCGCCTCGTC AGGAAAACCG AAGGCAACAT TACTGAGCAG GTAGAAGATG CAGTAGCTCA 781 TTACTCAATG TTGCCTTCCC AGGGCGGATA TCCATCACAC TGGCGGCCGC TCGAGCCCAG 841 CCCCGCCAGG CCCGCACTCC GCGCCCCGGC CTCCGCTACC AGTGGCAGCC GCAAGCGCGC 901 CCGCCCGCCC GCCGCCCCCG GACGCGACCA GGGCCAGGCC ACCGGCCCGC AGGAGACTGC 961 GGCTGTCGGT GGACGAGGTT TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCC 1021 TGCCCTTCTC CGGGCCAGAA GATAAAGAAT CCACCCCCGG GCAGCAAGGT CAGCCCGCCC 1081 CACCTGACAT CCCGCGGCAG GACCAGGACA CCATCTATCT AGAGGTTAAC ATCGAAGGAT 1141 CAGCTAATCG ATAATTCACC CTCTGGATTA CAAAATTTGG TGAAAGAAAT TGTGACACCG 1201 TGGGTAT // |
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| Primer: PRE_rev | RDB15267_A7H3b.seq |
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>D04498H7-1_A7H3_PRE_rev_C09_09_ABI24.ab1 1 TCGGTTCAAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT 61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTAGA 121 TGGTGTCCTG GTCCTGCGCG GATGTCAGGT GGGGCGGCTG ACCTGCTGCC GGGGTGGATT 181 TCTTTATCTT CTGGCCCGGA GAAGGGCAGG CTGGGATGTC TGGGGCCTCG GGGGTACTGG 241 GGCTGGAAAC CTCGTCCACC GACAGCCGCA GTCTCCTGCG GGCCGGTGGC CTGGCCTGGT 301 CGCGTCCGGG GGCGGCGGGC GGGCGGGCGC GCTTGCGGCT GCCACTGGTA GCGGAGGCCG 361 GGGCGCGGAG TGCGGGCCTG GCGGGGCTGG GCTCGAGCGG CCGCCAGTGT GATGGATATC 421 CGCCCTGGGA AGGCAACATT GAGTAATGAG CTACTGCATC TTCTACCTGC TCAGTAATGT 481 TGCCTTCGGT TTTCCTGACG AGGCGATGGC CGATGTAATG GTCTCCTGGC ATTTCAAGAA 541 TCTCTTTTGC CGCCTTGTAA GTAGTCTTCA TTTGGCATTT GTGATTGCCG CCTCCTTCAA 601 GTTTTAGGTA CATCGTAACA TCACCCTTCA GAACTCCGTC GCTGGCAGTA ATTTTCTCGG 661 TTGATGGCTC CCAATCAACA CTTTGGTTTT GCATGATAGG ACCATCGGCA GGAAAGTTAA 721 CCCCAGTAAA TTTGGATTTG TGGTAGAAGG TGTTTCCTCT AAGGCTTATA TGCGCACTGA 781 CTGAAGCGGA CCCACCATCT TCGAACTCCA ACGACCTTTC CCATGACAGG CCTTCAGGAA 841 ATGCTTGTTT GAAATAGTCT GGTATCTCTT CTGGATATTT AGTAAATACT CTGTGGCCGT 901 AACAGAACAC GTGTGACACT AAGTCAAACG CGAAAGGCAT TGGCCCGCCC TCGGGCCATT 961 GTGACGCGTA GTGTCATCTC TTGATGTCCC TCGTAAAGGT CTGCCTGTGC CCTTCACCTT 1021 TCAATTGTGA ACTCATGCCC ATTGACGGAG CCGTCCATGT AGTACCTCCA TCCTCATCTC 1081 TGGGTTTAAT CACACTTCAC CATTGCTTGA CCGACTTCCT CGAGACCGGT AGCGGTGGTT 1141 TCCACCGAAA CACACCCCCC // |
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| Primer: CMV-Forward | RDB15267_A7H3c.seq |
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>D04498H7-1_A7H3_CMV-Forward_D09_12_ABI24.ab1 1 TGTTACACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT 121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA 181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA 241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA 301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG 361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT 421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG 481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG 541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA 601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT 661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT 721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG 781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT 841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG 901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA 961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTATTGTCT 1021 GGGTATAGTG CAGCAGCAGA ACAATTTGCT GAGGGCTATT TGAGGCGCAA CCAGCCATCT 1081 GGTGGCACTC ACAGTCTGGG CAATCAGGCA GCTCAGCCAA GGAATCTGGG CTGGTGGGAA 1141 AGAAATAACC TTAG // |
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| Primer: BGH_rev2 | RDB15267_A7H3d.seq |
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>D04498H7-1_A7H3_BGH_rev2_A04_01_ABI24.ab1 1 CTCTCACGGC CAGAGGCACG GGGGAGGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC 61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA 121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA 181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT 241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG 301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT 361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT 421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT 481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT 541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC 601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG 661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC 721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC 781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC 841 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG TCCGCTGGGA 901 TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG 961 GCGAGCAGCC ATGGAAAGGA CGTCAGCTTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC 1021 AACAGCCCGA GCCCCTGTCC AGCAGCGGGC AAGCAGCGGC GATGAGTTCC GCCGTTGGCC 1081 AATAGGGAGG GGGAAGGCGA AGGTTCCGAG GGACCTGACA GGTGGTGCCA TGCCTAACCA 1141 GATGGGGGGG TGTGCGTCCA C // |
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| Primer: SV40pro_F | RDB15267_A7H3e.seq |
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>D04498H7-1_A7H3_SV40pro_F_A05_02_ABI24.ab1 1 CCTCCGGTTC CTGTGGCATT CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT 61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA 121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT 181 GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA 241 GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT 301 GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA 361 CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT 421 CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC 481 GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA 541 GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 601 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 661 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 721 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 781 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT 841 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 901 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 961 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 1021 GCATTAATGA AATCGCCCAA CGCGCGGGGA GAAGGCGGTT TGCGTATGGG GCGCTCTTCC 1081 GCTTCTCGCT CACTGACTCC GCTGCGCTCG TCGTCGGCTG CCGGCCGAGC CGTATTCAGG 1141 CTCCACCTCA AAGGGCCGGT AATTAACCGG GCT // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078. |
|---|
2019.01.08
GNP_filter3_RDBDEP_html_181012.pl