Please review the QC test results indicated by icon below as well as clone information before placing your order.

mKO2-hCdt1(30/120) / pCSII-EF

Lentivirus vector plasmid of Fucci-G1 phase probe. Component of Fucci cell cycle probe.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A. et al. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell, 132 (3): 487-498. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution	The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A. et al. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell, 132 (3): 487-498. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
付加的提供条件	レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	【追加提供依頼書】受入れ確認書(書式J) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15267_A7H3p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Region: EF-1a pro,insert5' Sequence file: RDB15267_A7H3a.seq
>D04498H7-1_A7H3_EF1a-C_F_A01_01_ABI24.ab1     1 TTCTTGTGGA ATTTGGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCCAGAC    61 AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA ACACGCTACC GGTCTCGAGA   121 ATTCGCCACC ATGGTGAGTG TGATTAAACC AGAGATGAAG ATGAGGTACT ACATGGACGG   181 CTCCGTCAAT GGGCATGAGT TCACAATTGA AGGTGAAGGC ACAGGCAGAC CTTACGAGGG   241 ACATCAAGAG ATGACACTAC GCGTCACAAT GGCCGAGGGC GGGCCAATGC CTTTCGCGTT   301 TGACTTAGTG TCACACGTGT TCTGTTACGG CCACAGAGTA TTTACTAAAT ATCCAGAAGA   361 GATACCAGAC TATTTCAAAC AAGCATTTCC TGAAGGCCTG TCATGGGAAA GGTCGTTGGA   421 GTTCGAAGAT GGTGGGTCCG CTTCAGTCAG TGCGCATATA AGCCTTAGAG GAAACACCTT   481 CTACCACAAA TCCAAATTTA CTGGGGTTAA CTTTCCTGCC GATGGTCCTA TCATGCAAAA   541 CCAAAGTGTT GATTGGGAGC CATCAACCGA GAAAATTACT GCCAGCGACG GAGTTCTGAA   601 GGGTGATGTT ACGATGTACC TAAAACTTGA AGGAGGCGGC AATCACAAAT GCCAAATGAA   661 GACTACTTAC AAGGCGGCAA AAGAGATTCT TGAAATGCCA GGAGACCATT ACATCGGCCA   721 TCGCCTCGTC AGGAAAACCG AAGGCAACAT TACTGAGCAG GTAGAAGATG CAGTAGCTCA   781 TTACTCAATG TTGCCTTCCC AGGGCGGATA TCCATCACAC TGGCGGCCGC TCGAGCCCAG   841 CCCCGCCAGG CCCGCACTCC GCGCCCCGGC CTCCGCTACC AGTGGCAGCC GCAAGCGCGC   901 CCGCCCGCCC GCCGCCCCCG GACGCGACCA GGGCCAGGCC ACCGGCCCGC AGGAGACTGC   961 GGCTGTCGGT GGACGAGGTT TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCC  1021 TGCCCTTCTC CGGGCCAGAA GATAAAGAAT CCACCCCCGG GCAGCAAGGT CAGCCCGCCC  1081 CACCTGACAT CCCGCGGCAG GACCAGGACA CCATCTATCT AGAGGTTAAC ATCGAAGGAT  1141 CAGCTAATCG ATAATTCACC CTCTGGATTA CAAAATTTGG TGAAAGAAAT TGTGACACCG 1201 TGGGTAT //
Primer: PRE_rev Region: PRE,insert3' Sequence file: RDB15267_A7H3b.seq
>D04498H7-1_A7H3_PRE_rev_C09_09_ABI24.ab1     1 TCGGTTCAAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT    61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTAGA   121 TGGTGTCCTG GTCCTGCGCG GATGTCAGGT GGGGCGGCTG ACCTGCTGCC GGGGTGGATT   181 TCTTTATCTT CTGGCCCGGA GAAGGGCAGG CTGGGATGTC TGGGGCCTCG GGGGTACTGG   241 GGCTGGAAAC CTCGTCCACC GACAGCCGCA GTCTCCTGCG GGCCGGTGGC CTGGCCTGGT   301 CGCGTCCGGG GGCGGCGGGC GGGCGGGCGC GCTTGCGGCT GCCACTGGTA GCGGAGGCCG   361 GGGCGCGGAG TGCGGGCCTG GCGGGGCTGG GCTCGAGCGG CCGCCAGTGT GATGGATATC   421 CGCCCTGGGA AGGCAACATT GAGTAATGAG CTACTGCATC TTCTACCTGC TCAGTAATGT   481 TGCCTTCGGT TTTCCTGACG AGGCGATGGC CGATGTAATG GTCTCCTGGC ATTTCAAGAA   541 TCTCTTTTGC CGCCTTGTAA GTAGTCTTCA TTTGGCATTT GTGATTGCCG CCTCCTTCAA   601 GTTTTAGGTA CATCGTAACA TCACCCTTCA GAACTCCGTC GCTGGCAGTA ATTTTCTCGG   661 TTGATGGCTC CCAATCAACA CTTTGGTTTT GCATGATAGG ACCATCGGCA GGAAAGTTAA   721 CCCCAGTAAA TTTGGATTTG TGGTAGAAGG TGTTTCCTCT AAGGCTTATA TGCGCACTGA   781 CTGAAGCGGA CCCACCATCT TCGAACTCCA ACGACCTTTC CCATGACAGG CCTTCAGGAA   841 ATGCTTGTTT GAAATAGTCT GGTATCTCTT CTGGATATTT AGTAAATACT CTGTGGCCGT   901 AACAGAACAC GTGTGACACT AAGTCAAACG CGAAAGGCAT TGGCCCGCCC TCGGGCCATT   961 GTGACGCGTA GTGTCATCTC TTGATGTCCC TCGTAAAGGT CTGCCTGTGC CCTTCACCTT  1021 TCAATTGTGA ACTCATGCCC ATTGACGGAG CCGTCCATGT AGTACCTCCA TCCTCATCTC  1081 TGGGTTTAAT CACACTTCAC CATTGCTTGA CCGACTTCCT CGAGACCGGT AGCGGTGGTT 1141 TCCACCGAAA CACACCCCCC //
Primer: CMV_Forward Region: CMV pro,5'LTR,HIV-1 psi,RRE Sequence file: RDB15267_A7H3c.seq
>D04498H7-1_A7H3_CMV-Forward_D09_12_ABI24.ab1     1 TGTTACACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT   121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA   181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA   241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA   301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG   361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT   421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG   481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG   541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA   601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT   661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT   721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG   781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT   841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG   901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA   961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTATTGTCT  1021 GGGTATAGTG CAGCAGCAGA ACAATTTGCT GAGGGCTATT TGAGGCGCAA CCAGCCATCT  1081 GGTGGCACTC ACAGTCTGGG CAATCAGGCA GCTCAGCCAA GGAATCTGGG CTGGTGGGAA 1141 AGAAATAACC TTAG //
Primer: cPPT_CTS-E_R Region: cPPT_CTS,RRE Sequence file: RDB15267_A7H3f.seq
>15268_15268_B0B4F_cPPT_CTS-E_R_F02_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCT TTAGTTTGTA TGTCTGTTGC TATTATGTCT    61 ACTATTCTTT CCCCTGCACT GTACCCCCCA ATCCCCCCTT TTCTTTTAAA ATTGTGGATG   121 AATACTGCCA TTTGTAGATC CGTTCACTAA TCGAATGGAT CTGTCTCTGT CTCTCTCTCC   181 ACCTTCTTCT TCTATTCCTT CGGGCCTGTC GGGTCCCCTC GGGGTTGGGA GGTGGGTCTG   241 AAACGATAAT GGTGAATATC CCTGCCTAAC TCTATTCACT ATAGAAAGTA CAGCAAAAAC   301 TATTCTTAAA CCTACCAAGC CTCCTACTAT CATTATGAAT AATTTTATAT ACCACAGCCA   361 ATTTGTTATG TTAAACCAAT TCCACAAACT TGCCCATTTA TCTAATTCCA ATAATTCTTG   421 TTCATTCTTT TCTTGCTGGT TTTGCGATTC TTCAATTAAG GAGTGTATTA AGCTTGTGTA   481 ATTGTTAATT TCTCTGTCCC ACTCCATCCA GGTCGTGTGA TTCCAAATCT GTTCCAGAGA   541 TTTATTACTC CAACTAGCAT TCCAAGGCAC AGCAGTGGTG CAAATGAGTT TTCCAGAGCA   601 ACCCCAAATC CCCAGGAGCT GTTGATCCTT TAGGTATCTT TCCACAGCCA GGATTCTTGC   661 CTGGAGCTGC TTGATGCCCC AGACTGTGAG TTGCAACAGA TGCTGTTGCG CCTCAATAGC   721 CCTCAGCAAA TTGTTCTGCT GCTGCACTAT ANCAGACAAT AATTGTCTGG CCTGTACCGT   781 CNGCGTCATT GANGCTGCGC CCATAGTGCT TCCTGCTGCT CCCNAGAACN CAAGGANCAA   841 AGCTNCTATT NCNACNGCTC TTTTTTTCTC TCTGCNNNAC TCNNNTCTTN GCCTTNNNGG   901 GNGCNACNNC TNANGGTNCN NTTTTTACTA CTTTATATTT ATATNANNCA CTTCTCNNAN 961 NNNNCCNNNA NATNNNCNNN NNNNNNN //
Primer: BGH_rev2 Region: bGHp(A),3'LTR,PRE Sequence file: RDB15267_A7H3d.seq
>D04498H7-1_A7H3_BGH_rev2_A04_01_ABI24.ab1     1 CTCTCACGGC CAGAGGCACG GGGGAGGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC    61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA   121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA   181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT   241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG   301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT   361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT   421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT   481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT   541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC   601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG   661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC   721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC   781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC   841 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG TCCGCTGGGA   901 TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG   961 GCGAGCAGCC ATGGAAAGGA CGTCAGCTTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC  1021 AACAGCCCGA GCCCCTGTCC AGCAGCGGGC AAGCAGCGGC GATGAGTTCC GCCGTTGGCC  1081 AATAGGGAGG GGGAAGGCGA AGGTTCCGAG GGACCTGACA GGTGGTGCCA TGCCTAACCA 1141 GATGGGGGGG TGTGCGTCCA C //
Primer: SV40pro_F Region: SV40 pro_ori,Zeo,SV40p(A) Sequence file: RDB15267_A7H3e.seq
>D04498H7-1_A7H3_SV40pro_F_A05_02_ABI24.ab1     1 CCTCCGGTTC CTGTGGCATT CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT    61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA   121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT   181 GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA   241 GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT   301 GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA   361 CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT   421 CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC   481 GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA   541 GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT   601 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT   661 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG   721 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT   781 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT   841 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA   901 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT   961 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT  1021 GCATTAATGA AATCGCCCAA CGCGCGGGGA GAAGGCGGTT TGCGTATGGG GCGCTCTTCC  1081 GCTTCTCGCT CACTGACTCC GCTGCGCTCG TCGTCGGCTG CCGGCCGAGC CGTATTCAGG 1141 CTCCACCTCA AAGGGCCGGT AATTAACCGG GCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl