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mKO2-hCdt1(30/120) / pCSII-EF (#RDB15267)

Lentivirus vector plasmid of Fucci-G1 phase probe. Component of Fucci cell cycle probe.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Sakaue-Sawano, A., Cell 132 (3): 487-498 (2008). PMID 18267078. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name FUCCI-Red
Clone info. Lentivirus vector plasmid of Fucci-G1 phase probe. One of the component of Fucci cell cycle probe. Fluorescence was detected in the nucleus.
Vector backbone CSII-EF-MCS (RDB04378) (Plasmid)
Size of vector backbone 9.1 kb
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 37℃, LB + Amp
Gene/insert name Human CDT1 cDNA Fungia concinna OFP cDNA
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Sakaue-Sawano, A. et al., Cell 132 (3): 487-498 (2008).
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.

Further transfer, provision of services or resale of the Zeocin resistance gene, and the Deposited Materials is prohibited without InvivoGen's prior written consent. In addition, InvivoGen's Limited Use Label License applies to your use of this product.
Remarks Remember that you will be working with samples containing infectious virus.
Zeocin® is a trademark of InvivoGen registered with the United States Patent and Trademark Office and foreign equivalents. See InvivoGen Limited Use Label License.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Sakaue-Sawano, A. et al., Cell 132 (3): 487-498 (2008).
付加的使用条件：
レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。

InvivoGen社の事前の書面による同意がない限り、ゼオシン耐性遺伝子および寄託マテリアルの第三者への移転、サービスの提供、または再販売は禁止されています。また、ご利用に際してInvivoGen社のLimited Use Label Licenseが適用されます。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
提供条件は英文のまま記載してください。
ZeocinはInvivoGen社の製品です。Zeocin®は、米国特許商標庁および米国外の同等機関に登録されたInvivoGen社の商標です。InvivoGen Limited Use Label Licenseをご確認ください。

Catalog # Resource name Shipping form Fee
RDB15267 mKO2-hCdt1(30/120) / pCSII-EF DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The mKO2-hCdt1(30/120) / pCSII-EF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15267).

Reference section:

Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Osawa, H., Kashiwagi, S., Fukami, K., Miyata, T., Miyoshi, H., Imamura, T., Ogawa, M., Masai, H., Miyawaki, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078. [PubMed] [Article] [RRC of NBRP]
reference Möller, J., Process-induced cell cycle oscillations in CHO cultures: Online monitoring and model-based investigation. Biotechnol. Bioeng. 116 (11): 2931-2943 (2019). PMID 31342512. [PubMed] [Article]
reference Takahashi, H., Significance of Polypyrimidine Tract-Binding Protein 1 Expression in Colorectal Cancer. Mol. Cancer. Ther. 14 (7): 1705-1716 (2015). PMID 25904505. [PubMed] [Article] [RRC of NBRP]
reference Beaumont, K.A., Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids. J. Vis. Exp. (106):e53486 (2015). PMID 26779761. [PubMed] [Article]
reference Kagawa, Y., Cell Cycle-Dependent Rho GTPase Activity Dynamically Regulates Cancer Cell Motility and Invasion In Vivo. PLoS One, 8 (12): e83629 (2013). PMID 24386239. [PubMed] [Article] [RRC of NBRP]
user_report Halikar, A.M., Bax- Bcl-xL interaction dynamics during the progression of cell cycle and cell death using FLIM-FRET. Cell Stress 9: 143-157 (2025). PMID 40703333. [PubMed] [Article] [RRC of NBRP]
user_report Göbl, C., Cysteine oxidation triggers amyloid fibril formation of the tumor suppressor p16INK4A. Redox Biol. 28: 101316 (2020). PMID 31539802. [PubMed] [Article] [RRC of NBRP]
user_report Tanaka, A., Reversible expansion of pancreatic islet progenitors derived from human induced pluripotent stem cells. Genes Cells 25 (5): 302-311 (2020). PMID 32065490. [PubMed] [Article] [RRC of NBRP]
user_report Onozato, Y., Radiosensitivity of quiescent and proliferating cells grown as multicellular tumor spheroids. Cancer Sci. 108 (4): 704-712 (2017). PMID 28135769. [PubMed] [Article] [RRC of NBRP]
user_report Sereti, K.I., Methods to study the proliferation and differentiation of cardiac side population (CSP) cells. Methods Mol. Biol. 1036: 95-106 (2013). PMID 23807790. [PubMed] [Article] [RRC of NBRP]