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mVenus-hGeminin(1/110)/pcDNA3

Expression vector of Fucci2-S/G2/M phase probe. Component of Fucci2 cell cycle probe.

Catalog number RDB15458
Resource name mVenus-hGeminin(1/110)/pcDNA3
Clone info. Expression vector of Fucci2-S/G2/M phase probe, S/G2/M-Y(N). One of the component of Fucci2 cell cycle probe. Fluorescent was detected in the nucleus.
For the composition, refer Fig.1 of Sakaue-Sawano, A., Chem. Biol. 15 (12): 1243-1248 (2008).
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Human Geminin cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AB512479 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
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External Database
Human Geminin Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
          Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15458 mVenus-hGeminin(1/110)/pcDNA3 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962.
          original Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15458_A7Jvp1-4.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,MCS,insert5'
          Sequence file: RDB15458_A7Jva.seq check
          >D05018B9-2_A7Jv_CMV-Forward_E03_15_ABI24.ab1
              1 CTTTCCACTT CCGCCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
            121 ACGACTCACT ATAGGGAGAC CCAAGCTTGG TACCGAGCTC GGATCCACTA GTAACGGCCG
            181 CCAGTGTGCT GGAATTCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
            241 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG
            301 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC
            361 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT
            421 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC
            481 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT
            541 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG
            601 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCACCG
            661 CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGACG
            721 GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
            781 TGCTGCCCGA CAACCACTAC CTGAGCTACC AGTCCAAGCT GAGCAAAGAC CCCAACGAGA
            841 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG
            901 ACGAGCTGTA CAAGGGATAT CCATCACACT GGCGGCCCGC TCGAGATGAA TCCCAGTATG
            961 AAGCAGAAAC AAGAAAGAAT CAAAGAGAAT ATAAGAAATA GTTCTGTCCC AAGAAGAACC
           1021 TCTGAAGATG AATTCAGCCT TCCTGCATCT GGGATCCTCC TTGTTTGGAA AGAGGAAAAT
           1081 GAGCCTGTCC CGCCAGGGCC TTGGTCCACA GGGAACCATC CGGGAATGAA CCCACCTACC
          1141 ATTCTACAGA CCTTCCAAGC CCCTTGCGAG ATTAAAGATG
          //
          Primer: BGH_rev2
          Region: bGHp(A),insert3'
          Sequence file: RDB15458_A7Jvb.seq check
          >D05018B9-2_A7Jv_BGH_rev2_F03_18_ABI24.ab1
              1 CTTTTGGGTT TAGAGGCACG GGGGAGGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC
             61 AGTCGAGGCT GATCAGCGAG CTCTAGCATT TAGGTGACAC TATAGAATAG GGCCCTCTAG
            121 ATTACAGCGC CTTTCTCCGT TTTTCTGCCA CTTCCTTCCA ATACTGAGAG GATGGATTTT
            181 CTTTAATCAT AAGATCAAAT GACTCCTGGG TGACTCCTCC AAGATTTTTA TTTTCACTAG
            241 ATTCTGGGAC AATAACCCCA GGGCTGGAAG TTGTAGATGT TAAGTGGTCA TTCCGATGTT
            301 TCCTTTTGGA CAAGCCTGCG GACAGCTCAT TTTCTCTTCC AACAAGAGAT CCAGATGCAG
            361 AAGGCTGAAT CATCTTCAGA GTTCTTCTTG GGACAGAACT ATTCTTTATA TTCTCTTTGA
            421 TTTCTTCTTG TTTCTGCTTC ATACTGGGAT TCATCTCGAG CGGCCGCCAG TGTGATGGAT
            481 ATCCCTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG AACTCCAGCA
            541 GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG CTTGGACTGG TAGCTCAGGT
            601 AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC TGGTAGTGGT
            661 CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTG GCCTTGATGC
            721 CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG TACTCCAGCT
            781 TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG ATGCGGTTCA
            841 CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA
            901 AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG AAGTCGTGCT
            961 GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG GTGGTCACGA
           1021 GGTTGGGCCA GGGCACGGCA GCTTTGCCGG TGGTGCAGAT CAGCTTCAGG TCAGCTTGCC
           1081 CGTAGGTGGC ATCGACTTCG GTCCCTTCGC GGGACCACGG CTGGAAACTT GGTGGGCCGA
          1141 TTTTAACCGA TTCG
          //
          Primer: SV40pro_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB15458_A7Jvc.seq check
          >D05018B9_2_A7Jv_SV40pro_F_B07_04_ABI24.ab1
              1 GCTGTCTTGC TTTCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
             61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT
            121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
            181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
            241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
            301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
            361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
            421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
            481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
            541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
            601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
            661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
            721 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
            781 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
            841 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
            901 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGAAA TGACCGACCA AGCGACGCCC
            961 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCCTTC TATGAAAGGT TGGGCTTCGG
           1021 AATCGTTTTT CCCGGACGCC GGCTGGGATG ATCCCTTCAG CGCGGGGGAT CTCATGCTGG
          1081 GAGCTCATCG ACCCACCCGA CCTTGCTTTA TTGCAAGCCT TATTAATTGG TTTACA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962.
          original Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.

          2021.11.01

          GNP_filter3_RDBDEP_html_211029.pl