RDB15458 - mVenus-hGeminin(1/110)/pcDNA3

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

mVenus-hGeminin(1/110)/pcDNA3 (#RDB15458)

Expression vector of Fucci2-S/G2/M phase probe. Component of Fucci2 cell cycle probe.

Clone info.	Expression vector of Fucci2-S/G2/M phase probe, S/G2/M-Y(N). One of the component of Fucci2 cell cycle probe. Fluorescent was detected in the nucleus. For the composition, refer Fig.1 of Sakaue-Sawano, A., Chem. Biol. 15 (12): 1243-1248 (2008).
Vector backbone	pcDNA3 (plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name	Human Geminin cDNA Aequorea victoria GFP cDNA
Reference of insert sequence	AB512479 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考	提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15458	mVenus-hGeminin(1/110)/pcDNA3	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mVenus-hGeminin(1/110)/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15458).

Reference section:

Sakaue-Sawano, A., Kobayashi, T., Ohtawa, K., Miyawaki, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]

Sakaue-Sawano, A., Ohtawa, K., Hama, H., Kawano, M., Ogawa, M., Miyawaki, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15458_A7Jvp1-5.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: MCS,insert 5' Sequence file: RDB15458_A7Jvd.seq
>D05018B9-2_A7Jv_CMV-Forward_E03_15_ABI24.ab1 1 NNNNNNNNNN NNNNNNCNNT TGNNGCNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT 61 ATAAGCAGAG CTNNNNNNNN NNNNNNAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAN 121 ACNACTCACT ATAGGGAGAC CCAAGCTTGG TACCGAGCTC GGATCCACTA GTAACGGCCG 181 CCAGTGTGCT GGAATTCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA 241 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG 301 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC 361 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT 421 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC 481 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT 541 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG 601 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCACCG 661 CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGANG 721 GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC 781 TGCTGCCCGA CAACCACTAC CTGANCTACC AGTCCNAGCT GAGCAAAGAC CCCAACGANA 841 AGCGCGATCA CATGGTCCNG CTGNAGTTCG TGACCGCCGC CNGGATCACT CTCGGCNNGG 901 ACGANCTGTA CAAGGGATAT CCNTCACACN NGCGGNCNNC NCNNNATGAA TNCNNGTNTG 961 AANCAGAANN NNNNANGNNN NNAAGANNNN ATAANNANTA GTTNNGNNCC NNGNNNNNNN 1021 NNN //
Primer: BGH_rev2 (Pr0606) Region: insert 3' Sequence file: RDB15458_A7Jve.seq
>D05018B9-2_A7Jv_BGH_rev2_F03_18_ABI24.ab1 1 NNNNNNNNNN NNGNNNNNNG GGGGNNGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC 61 AGTCGAGGCT GANNNNNNNN CTCTAGCATT TAGGTGACAC TATAGAATAG GGCCCTCTAG 121 ATTACAGCGC CTTTCTCCGT TTTTCTGCCA CTTCCTTCCA ATACTGAGAG GATGGATTTT 181 CTTTAATCAT AAGATCAAAT GACTCCTGGG TGACTCCTCC AAGATTTTTA TTTTCACTAG 241 ATTCTGGGAC AATAACCCCA GGGCTGGAAG TTGTAGATGT TAAGTGGTCA TTCCGATGTT 301 TCCTTTTGGA CAAGCCTGCG GACAGCTCAT TTTCTCTTCC AACAAGAGAT CCAGATGCAG 361 AAGGCTGAAT CATCTTCAGA GTTCTTCTTG GGACAGAACT ATTCTTTATA TTCTCTTTGA 421 TTTCTTCTTG TTTCTGCTTC ATACTGGGAT TCATCTCGAG CGGCCGCCAG TGTGATGGAT 481 ATCCCTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG AACTCCAGCA 541 GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG CTTGGACTGG TAGCTCAGGT 601 AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC TGGTAGTGGT 661 CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTG GCCTTGATGC 721 CGTTCTTCTG CTTGTCGGCG GTGNTATAGA CGTTGTGGCT GTTGTAGTTG TACTCCAGCT 781 TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC NTTCAGCTCG ATGCGGTTCA 841 CCAGGGTGTC GCCCTCNNAC TTCACCTCGG CGCGGGTCTN GTANTTGCCG NCGTCCNNGA 901 AGAAGANGGT GCGCTCCTGN ANNNANCCTT CGGGCATGGC GGANTTNNAG AANNCGTGCT 961 GCTTCATNTG GNCNGGGNAN CGGGCGNNNC NCNNCNNNNN NNNNN //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori,NeoR Sequence file: RDB15458_A7Jvf.seq
>D05018B9_2_A7Jv_SV40pro_F_B07_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNANNN GTGNNGNNGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 721 AATGGCCGCT TTTCTGGANT CATCGACTGT GGNCGGCTGG GTGTGGCGGA NCGCTATCAG 781 GACATAGCGT TGGCTANNCG TGATATTGCT GAAGAGCTTG GCGGCGAATG NNCTGANCGC 841 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATNCGCAGC GCATCGCCTT CTATCGCCNT 901 CTTGANGANT TCTTCTGANC GGGANNNNGG GGGTTCNAAN NGNNNNANNN ANCGACGCCN 961 NNCNNNNNNT CNCNANNNTT CGATTNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]
original	Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. [link to RRC of NBRP]

Resource search

Link

Other resources in our bank

human Geminin

External Database
Human Geminin

Aequorea victoria GFP

Reference sequence