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mVenus-hGeminin(1/110)/pcDNA3

Expression vector of Fucci2-S/G2/M phase probe. Component of Fucci2 cell cycle probe.

Catalog number RDB15458
Resource name mVenus-hGeminin(1/110)/pcDNA3
Clone info. Expression vector of Fucci2-S/G2/M phase probe, S/G2/M-Y(N). One of the component of Fucci2 cell cycle probe. Fluorescent was detected in the nucleus.
For the composition, refer Fig.1 of Sakaue-Sawano, A., Chem. Biol. 15 (12): 1243-1248 (2008).
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Human Geminin cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AB512479 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank human GMNN (NCBI Gene 51053) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
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RDB15458 mVenus-hGeminin(1/110)/pcDNA3 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962.
original Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15458_A7Jvp1-4.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,MCS,insert5'
Sequence file: RDB15458_A7Jva.seq check
>D05018B9-2_A7Jv_CMV-Forward_E03_15_ABI24.ab1
    1 CTTTCCACTT CCGCCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
  121 ACGACTCACT ATAGGGAGAC CCAAGCTTGG TACCGAGCTC GGATCCACTA GTAACGGCCG
  181 CCAGTGTGCT GGAATTCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
  241 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG
  301 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC
  361 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT
  421 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC
  481 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT
  541 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG
  601 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCACCG
  661 CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGACG
  721 GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
  781 TGCTGCCCGA CAACCACTAC CTGAGCTACC AGTCCAAGCT GAGCAAAGAC CCCAACGAGA
  841 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG
  901 ACGAGCTGTA CAAGGGATAT CCATCACACT GGCGGCCCGC TCGAGATGAA TCCCAGTATG
  961 AAGCAGAAAC AAGAAAGAAT CAAAGAGAAT ATAAGAAATA GTTCTGTCCC AAGAAGAACC
 1021 TCTGAAGATG AATTCAGCCT TCCTGCATCT GGGATCCTCC TTGTTTGGAA AGAGGAAAAT
 1081 GAGCCTGTCC CGCCAGGGCC TTGGTCCACA GGGAACCATC CGGGAATGAA CCCACCTACC
1141 ATTCTACAGA CCTTCCAAGC CCCTTGCGAG ATTAAAGATG
//
Primer: BGH_rev2
Region: bGHp(A),insert3'
Sequence file: RDB15458_A7Jvb.seq check
>D05018B9-2_A7Jv_BGH_rev2_F03_18_ABI24.ab1
    1 CTTTTGGGTT TAGAGGCACG GGGGAGGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGAG CTCTAGCATT TAGGTGACAC TATAGAATAG GGCCCTCTAG
  121 ATTACAGCGC CTTTCTCCGT TTTTCTGCCA CTTCCTTCCA ATACTGAGAG GATGGATTTT
  181 CTTTAATCAT AAGATCAAAT GACTCCTGGG TGACTCCTCC AAGATTTTTA TTTTCACTAG
  241 ATTCTGGGAC AATAACCCCA GGGCTGGAAG TTGTAGATGT TAAGTGGTCA TTCCGATGTT
  301 TCCTTTTGGA CAAGCCTGCG GACAGCTCAT TTTCTCTTCC AACAAGAGAT CCAGATGCAG
  361 AAGGCTGAAT CATCTTCAGA GTTCTTCTTG GGACAGAACT ATTCTTTATA TTCTCTTTGA
  421 TTTCTTCTTG TTTCTGCTTC ATACTGGGAT TCATCTCGAG CGGCCGCCAG TGTGATGGAT
  481 ATCCCTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG AACTCCAGCA
  541 GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG CTTGGACTGG TAGCTCAGGT
  601 AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC TGGTAGTGGT
  661 CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTG GCCTTGATGC
  721 CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG TACTCCAGCT
  781 TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG ATGCGGTTCA
  841 CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA
  901 AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG AAGTCGTGCT
  961 GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG GTGGTCACGA
 1021 GGTTGGGCCA GGGCACGGCA GCTTTGCCGG TGGTGCAGAT CAGCTTCAGG TCAGCTTGCC
 1081 CGTAGGTGGC ATCGACTTCG GTCCCTTCGC GGGACCACGG CTGGAAACTT GGTGGGCCGA
1141 TTTTAACCGA TTCG
//
Primer: SV40pro_F
Region: SV40 pro_ori,NeoR
Sequence file: RDB15458_A7Jvc.seq check
>D05018B9_2_A7Jv_SV40pro_F_B07_04_ABI24.ab1
    1 GCTGTCTTGC TTTCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
   61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT
  121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
  661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  721 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
  781 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
  841 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
  901 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGAAA TGACCGACCA AGCGACGCCC
  961 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCCTTC TATGAAAGGT TGGGCTTCGG
 1021 AATCGTTTTT CCCGGACGCC GGCTGGGATG ATCCCTTCAG CGCGGGGGAT CTCATGCTGG
1081 GAGCTCATCG ACCCACCCGA CCTTGCTTTA TTGCAAGCCT TATTAATTGG TTTACA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962.
original Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.

2020.03.05

GNP_filter3_RDBDEP_html_200228.pl