Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

mVenus-hGeminin(1/110)/pcDNA3 (#RDB15458)

Expression vector of Fucci2-S/G2/M phase probe. Component of Fucci2 cell cycle probe.

Clone info. Expression vector of Fucci2-S/G2/M phase probe, S/G2/M-Y(N). One of the component of Fucci2 cell cycle probe. Fluorescent was detected in the nucleus.
For the composition, refer Fig.1 of Sakaue-Sawano, A., Chem. Biol. 15 (12): 1243-1248 (2008).
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Growth conditions 37oC, LB + Amp
Gene/insert name Human Geminin cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AB512479 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15458hts01.seq checkAssembled from experimentally sequenced data.
Publication Sakaue-Sawano, A., BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]
Publication Sakaue-Sawano, A., Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. [link to RRC of NBRP]
Test sheet RDB15458_A8H9p1-5.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Sakaue-Sawano, A. et al., Chemistry & Biology 15: 1243-1248 (2008). Sakaue-Sawano, A. et al., BMC Cell Biology 12: 2 (2011).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Sakaue-Sawano, A. et al., Chemistry & Biology 15: 1243-1248 (2008). Sakaue-Sawano, A. et al., BMC Cell Biology 12: 2 (2011).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15458 mVenus-hGeminin(1/110)/pcDNA3 DNA solution

How to cite this biological resource

Materials & Methods section:

The mVenus-hGeminin(1/110)/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15458).

Reference section:

Sakaue-Sawano, A., Kobayashi, T., Ohtawa, K., Miyawaki, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]
Sakaue-Sawano, A., Ohtawa, K., Hama, H., Kawano, M., Ogawa, M., Miyawaki, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]
original Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. [link to RRC of NBRP]