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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mCherry-zCdt1(1/190) / pT2KXIGdeltain (#RDB15278)

zCdt1-based G1 marker for zebrafish cells to label nuclei with red fluorescent protein mCherry.

Clone info. zCdt1-based G1 marker for zebrafish cells to label nuclei with red fluorescent protein mCherry.
Comment Refer Fukuhara, S. et al., Dev. Biol. 393 (1): 10-23, 2014.
Vector backbone pT2KXIGdeltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC
Gene/insert name zebrafish zCdt1 cDNA Discosoma sp. RFP cDNA
Reference of insert sequence AB971706 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Sugiyama, M. et al., Proc. Natl. Acad. Sci. USA. 106 (49), 20812-20817 (2009).
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Sugiyama, M. et al., Proc. Natl. Acad. Sci. USA. 106 (49), 20812-20817 (2009).
付加的使用条件：
Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
備考 提供条件は英文のまま記載してください。
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15278 mCherry-zCdt1(1/190) / pT2KXIGdeltain DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mCherry-zCdt1(1/190) / pT2KXIGdeltain was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15278).

Reference section:

Fukuhara, S., Zhang, J., Yuge, S., Ando, K., Wakayama, Y., Sakaue-Sawano, A., Miyawaki, A., Mochizuki, N., Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev. Biol. 393 (1): 10-23 (2014). PMID 24975012. [link to RRC of NBRP]
Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, S., Miyawaki, A., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15278_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: beta-gi_F ()
Region: insert 5'
Sequence file: RDB15278_A7H9a.seq check
>D04498I9_A7H9_2_beta-gi_F_G06_07_ABI08.ab1
    1 CAGCATTCAT GCCTTCTTCT CTTTCCTACA GCTCCTGGGC AACGTGCTGG TTGTTGTGCT
   61 GTCTCATCAT TTTGGCAAAG AATTCCTCGA CGGATCCACT AGTAACGGCC GCCAGTGTGC
  121 TGGAATTCGC CACCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT
  181 TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG
  241 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG
  301 GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG
  361 CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT
  421 TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT
  481 CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT
  541 CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT
  601 ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG
  661 GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG
  721 GCGCCTACAA CGTCAACATC AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG
  781 TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA
  841 AGGGATATCC ATCACACTGG CGGCCGCTCG AGATGGCTCA AGCTCGTGTT ACTGATTATT
  901 TTGCACAGAG TAAAAAAGCT GGTGTTTCTC GATCTTTACG GTCTAAAGGG ACAAAAGGTG
  961 TCCGGGGATG TAGTAGAATC AGCGGTGATC AATAAACCGA GGAGTTCGAG CAGAGCGTCC
 1021 CGGTTCTTCA CGCAAAGCGA CGCACAGTGA GATCCACCAC AGCAGAGCCG CAGAAACAAC
 1081 TCCAGCTTGG GAGTTTCTCA AAGGTCATCG ACGGAGGCTT TAATCGGACC GAACCTGCCA
1141 GATACCGTCC GCGGATTCCG CCGGTGCACT GTGTGACACA GAACTCTGCG AAG
//
Primer: pAxCALNL_F1 ()
Region: insert 3'
Sequence file: RDB15278_A7H9b.seq check
>D04498I9_A7H9_2_pAxCALNL_F1_B07_04_ABI24.ab1
    1 TCATTCATCT TATCATGTCT GGATCATCAT CGATTACTCT TTGCGGGCAG TTTGTTGAGG
   61 TGCCTGAGGG CTGCTGCTGG CCAAGGGCTC AGTGGTCTTT ACTTTGTCGT CGCTTGCCAG
  121 TAGATAGAGT TTTTTCCTCG CAGTTTTCTG TCCAGCGCGC TCCTCCGGTG AGCTCCTGCA
  181 GTTGTGGCCC GCGTTGAGTC TCTGCCGCTT CTTGGCGCTG CTATGCAGCT CGGCTGTGGA
  241 CGGAAACAAC ACCGAACACA CGTCGAACTC CGGTGAACTC CGCTTAGGGG TCCTGGGACT
  301 CGCCGTTAAG CCCTCAATCT TCACATCACG CGAATCCGCG ACGGTATCTG CAGTCTCCGT
  361 CGATAAAGCC TCGTCGATGA CTTTGAGAAA CTCCAGCTGA GTTTGTTTCT GCGGCTCTGC
  421 TGTGGTGATC TCACTGTGCG TCGCTTTGCG TGAAGAACCG GACGCTCTGC TCGAACTCCT
  481 CGGTTTATTG ATCACCGCTG ATTCTACTAC ATCCCCGGAC ACCTTTTGTC CCTTAGACCG
  541 TAAAGATCGA GAAACACCAG CTTTTTTACT CTGTGCAAAA TAATCAGTAA CACGAGCTTG
  601 AGCCATCTCG AGCGGCCGCC AGTGTGATGG ATATCCCTTG TACAGCTCGT CCATGCCGCC
  661 GGTGGAGTGG CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG
  721 GGAGGTGATG TCCAACTTGA TGTTGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT
  781 GGCCTTGTAG GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT
  841 CTGCTTGATC TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCGG AGGAGGCCTC
  901 CCAGCCCATG GTCTTCTTCT GCATTACGGG GCCGTCGGAA GGGGAAGTTG GGTGCCGCGC
  961 AGCTTTCACC TTGTAGATGA CTCGCCGTCC TGCAGGAGAA GTCTGGTCAC GTCACACGCG
 1021 CGTCTCGAGT CATCACGCGC TCACTGAAGC CGTCGGTAGA CGCTCAGTAG TCGGATGTCG
1081 CCGGGGCATG C
//
Primer: EF1a-N_R2 ()
Region: Tol2 R
Sequence file: RDB15278_A7H9c.seq check
>D04498I9_A7H9_2_EF1a-N_R2_C07_07_ABI24.ab1
    1 GTTTAGCGGG CAAAGAATTG GCAGTTTGAT TTGCATTGGA AAGGGTCGCT GGCTTTTGTG
   61 TTACACGCCC CTTATTTGTG CTTGATTAGA TGATCCCCCT GCTCGAGGGC CCATCTGGCC
  121 TGTGTTTCAG ACACCAGGGA GTCTCTGCTC ACGTTTCCTG CTATTTGCAG CCTCTCTATC
  181 AAGACTAATA CACCTCTTCC CGCATCGGCT GCCTGTGAGA GGCTTTTCAG CACTGCAGGA
  241 TTGCTTTTCA GCCCCAAAAG AGCTAGGCTT GACACTAACA ATTTTGAGAA TCAGCTTCTA
  301 CTGAAGTTAA ATCTGAGGTT TTACAACTTT GAGTAGCGTG TACTGGCATT AGATTGTCTG
  361 TCTTATAGTT TGATAATTAA ATACAAACAG TTCTAAAGCA GGATAAAACC TTGTATGCAT
  421 TTCATTTAAT GTTTTTTGAG ATTAAAAGCT TAAACAAGAA TCTCTAGTTT TCTTTCTTGC
  481 TTTTACTTTT ACTTCCTTAA TACTCAAGTA CAATTTTAAT GGAGTACTTT TTTACTTTTA
  541 CTCAAGTAAG ATTCTAGCCA GATACTTTTA CTTTTAATTG AGTAAAATTT TCCCTAAGTA
  601 CTTGTACTTT CACTTGAGTA AAATTTTTGA GTACTTTTTA CACCTCTGTC AAGAACTCCT
  661 GGACAAACCT CTGACCTGTG TGGAACAGAG TGGATATGGG TGTCTGAACA GATATTCACG
  721 TCTTTTGCAG ATCAGAGGGC ATTTCTGGTG TTGTGCAATG ACCTGGGTCC AACAGGTAGG
  781 CGTACTCGTA TCCAAGAGCA TTGTTGGACA GGAGGTAGTC TCCATTCCTA TAAAGAGGAA
  841 GGAATGGCAC CATGTAGTAG CCGTCATTGT GGCCAATTGG TGCATTGGTA CGTGGGTAGA
  901 TGGACCGGGG AGGCTGATGA GTTCTTAGCC AGCGCTCAAA GATGCTAGAA AATGATGTAA
  961 GAAACAAATA TGCAAGATGA AAAGTTAAGA TAAAGAATAA AAAAATTAAC AAATAAATGG
 1021 CAATTAATGG TTTTGTGTTC TAATTCCAAC CAATAATATC CTTCACGGCA GTAAAGGCCC
1081 ACCAGGAATA TTTTTTGTTT CTTGCTCTGC AGGGGCCAAC CAAACTCCTT AAC
//
Primer: pAxEF_R2 ()
Region: Tol2 L
Sequence file: RDB15278_A7H9d.seq check
>D04498I9_A7H9_2_pAxEF_R2_D07_10_ABI24.ab1
    1 GGAGGTTTAA AATGGCCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA
   61 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC
  121 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
  181 ATTATGATCC TCTAGATCAG ATCTGCGAAG ATACGGCCAC GGGTGCTCTT GATCCTGTGG
  241 CTGATTTTGG ACTGTGCTGC TCGCAGCTGC TGATGAATCA CATACTTCCT CCATTTTCTT
  301 CCACTGATTG ACTGTTATAA TTTCCCTAAT TTCCAGGTCA AGGTGCTGTG CATTGTGGTA
  361 ATAGATGTGA CATGACGTCA CTTCCAAAGG ACCAATGAAC ATGTCTGACC AATTTCATAT
  421 AATGTGAAAA CGATTTTCAT AGGCAGAATA AATAACATTT AAATTAAACT GGGCATCAGC
  481 GCAATTCAAT TGGTTTGGTA ATAGCAAGGG AAAATAGAAT GAAGTGATCT CCAAAAAATA
  541 AGTACTTTTT GACTGTAAAT AAAATTGTAA GGAGTAAAAA GTACTTTTTT TTCTAAAAAA
  601 ATGTAATTAA GTAAAAGTAA AAGTATTGAT TTTTAATTGT ACTCAAGTAA AGTAAAAATC
  661 CCCAAAAATA ATACTTAAGT ACAGTAATCA AGTAAAATTA CTCAAGTACT TTACACCTCT
  721 GGTTCTTGAC CCCCTACCTT CAGCAAGCCC AGCAGATCCA CTAGTTCTAG AGCGGCCGCC
  781 ACCGCGGTGG AGCTCCAGCT TTTGTTCCCT TTAGTGAGGG TTAATTGCGC GCTTGGCGTA
  841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
  901 ACGAGCCGGA AGCATAAAAG TGTAAAGGCC TGGGGTGCCC TAATGAAGTG AGCTAACTCA
  961 CATTAAATTG CGTTGCGCTC ACTGCCCGCT TTTCCAGTCG GGAAACCCTG TCGTTGGCCA
 1021 GCTGGCATTA AATGAAATCG GGCCAACCGC GCGGGGGAAG AAGGGCGGTT TTGGCGTATT
 1081 TGGGCCGCTC CTTCGGCCTT TCCATCGCTC ACTGGACTTC GCTTGCGGCT TCGGGTTCGT
1141 TTTCGGACCT TGGCGGCGGA AGAGCGGGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuhara, S., Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev. Biol. 393 (1): 10-23 (2014). PMID 24975012. [link to RRC of NBRP]
original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]
reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.