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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mCherry-zCdt1(1/190) / pT2KXIGdeltain

zCdt1-based G1 marker for zebrafish cells to label nuclei with red fluorescent protein mCherry.

Catalog number RDB15278
Resource name mCherry-zCdt1(1/190) / pT2KXIGdeltain
Clone info. zCdt1-based G1 marker for zebrafish cells to label nuclei with red fluorescent protein mCherry.
Comment Refer Fukuhara, S. et al., Dev. Biol. 393 (1): 10-23, 2014.
Vector backbone pT2KXIGdeltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Gene/insert name zebrafish zCdt1 cDNA Discosoma sp. RFP cDNA
Reference of insert sequence AB971706 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
zebrafish zCdt1 Discosoma sp. RFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
          Additional terms and conditions for distribution The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
          付加的提供条件 Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 提供条件は英文のまま記載してください。
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15278 mCherry-zCdt1(1/190) / pT2KXIGdeltain DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Fukuhara, S., Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev. Biol. 393 (1): 10-23 (2014). PMID 24975012.
          original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Transposon and plasmid, Kawakami laboratory (English text)
          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15278_A7H9p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: beta-gi_F
          Region: insert 5'
          Sequence file: RDB15278_A7H9a.seq check
          >D04498I9_A7H9_2_beta-gi_F_G06_07_ABI08.ab1
              1 CAGCATTCAT GCCTTCTTCT CTTTCCTACA GCTCCTGGGC AACGTGCTGG TTGTTGTGCT
             61 GTCTCATCAT TTTGGCAAAG AATTCCTCGA CGGATCCACT AGTAACGGCC GCCAGTGTGC
            121 TGGAATTCGC CACCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT
            181 TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG
            241 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG
            301 GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG
            361 CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT
            421 TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT
            481 CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT
            541 CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT
            601 ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG
            661 GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG
            721 GCGCCTACAA CGTCAACATC AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG
            781 TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA
            841 AGGGATATCC ATCACACTGG CGGCCGCTCG AGATGGCTCA AGCTCGTGTT ACTGATTATT
            901 TTGCACAGAG TAAAAAAGCT GGTGTTTCTC GATCTTTACG GTCTAAAGGG ACAAAAGGTG
            961 TCCGGGGATG TAGTAGAATC AGCGGTGATC AATAAACCGA GGAGTTCGAG CAGAGCGTCC
           1021 CGGTTCTTCA CGCAAAGCGA CGCACAGTGA GATCCACCAC AGCAGAGCCG CAGAAACAAC
           1081 TCCAGCTTGG GAGTTTCTCA AAGGTCATCG ACGGAGGCTT TAATCGGACC GAACCTGCCA
          1141 GATACCGTCC GCGGATTCCG CCGGTGCACT GTGTGACACA GAACTCTGCG AAG
          //
          Primer: pAxCALNL_F1
          Region: insert 3'
          Sequence file: RDB15278_A7H9b.seq check
          >D04498I9_A7H9_2_pAxCALNL_F1_B07_04_ABI24.ab1
              1 TCATTCATCT TATCATGTCT GGATCATCAT CGATTACTCT TTGCGGGCAG TTTGTTGAGG
             61 TGCCTGAGGG CTGCTGCTGG CCAAGGGCTC AGTGGTCTTT ACTTTGTCGT CGCTTGCCAG
            121 TAGATAGAGT TTTTTCCTCG CAGTTTTCTG TCCAGCGCGC TCCTCCGGTG AGCTCCTGCA
            181 GTTGTGGCCC GCGTTGAGTC TCTGCCGCTT CTTGGCGCTG CTATGCAGCT CGGCTGTGGA
            241 CGGAAACAAC ACCGAACACA CGTCGAACTC CGGTGAACTC CGCTTAGGGG TCCTGGGACT
            301 CGCCGTTAAG CCCTCAATCT TCACATCACG CGAATCCGCG ACGGTATCTG CAGTCTCCGT
            361 CGATAAAGCC TCGTCGATGA CTTTGAGAAA CTCCAGCTGA GTTTGTTTCT GCGGCTCTGC
            421 TGTGGTGATC TCACTGTGCG TCGCTTTGCG TGAAGAACCG GACGCTCTGC TCGAACTCCT
            481 CGGTTTATTG ATCACCGCTG ATTCTACTAC ATCCCCGGAC ACCTTTTGTC CCTTAGACCG
            541 TAAAGATCGA GAAACACCAG CTTTTTTACT CTGTGCAAAA TAATCAGTAA CACGAGCTTG
            601 AGCCATCTCG AGCGGCCGCC AGTGTGATGG ATATCCCTTG TACAGCTCGT CCATGCCGCC
            661 GGTGGAGTGG CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG
            721 GGAGGTGATG TCCAACTTGA TGTTGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT
            781 GGCCTTGTAG GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT
            841 CTGCTTGATC TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCGG AGGAGGCCTC
            901 CCAGCCCATG GTCTTCTTCT GCATTACGGG GCCGTCGGAA GGGGAAGTTG GGTGCCGCGC
            961 AGCTTTCACC TTGTAGATGA CTCGCCGTCC TGCAGGAGAA GTCTGGTCAC GTCACACGCG
           1021 CGTCTCGAGT CATCACGCGC TCACTGAAGC CGTCGGTAGA CGCTCAGTAG TCGGATGTCG
          1081 CCGGGGCATG C
          //
          Primer: EF1a-N_R2
          Region: Tol2 R
          Sequence file: RDB15278_A7H9c.seq check
          >D04498I9_A7H9_2_EF1a-N_R2_C07_07_ABI24.ab1
              1 GTTTAGCGGG CAAAGAATTG GCAGTTTGAT TTGCATTGGA AAGGGTCGCT GGCTTTTGTG
             61 TTACACGCCC CTTATTTGTG CTTGATTAGA TGATCCCCCT GCTCGAGGGC CCATCTGGCC
            121 TGTGTTTCAG ACACCAGGGA GTCTCTGCTC ACGTTTCCTG CTATTTGCAG CCTCTCTATC
            181 AAGACTAATA CACCTCTTCC CGCATCGGCT GCCTGTGAGA GGCTTTTCAG CACTGCAGGA
            241 TTGCTTTTCA GCCCCAAAAG AGCTAGGCTT GACACTAACA ATTTTGAGAA TCAGCTTCTA
            301 CTGAAGTTAA ATCTGAGGTT TTACAACTTT GAGTAGCGTG TACTGGCATT AGATTGTCTG
            361 TCTTATAGTT TGATAATTAA ATACAAACAG TTCTAAAGCA GGATAAAACC TTGTATGCAT
            421 TTCATTTAAT GTTTTTTGAG ATTAAAAGCT TAAACAAGAA TCTCTAGTTT TCTTTCTTGC
            481 TTTTACTTTT ACTTCCTTAA TACTCAAGTA CAATTTTAAT GGAGTACTTT TTTACTTTTA
            541 CTCAAGTAAG ATTCTAGCCA GATACTTTTA CTTTTAATTG AGTAAAATTT TCCCTAAGTA
            601 CTTGTACTTT CACTTGAGTA AAATTTTTGA GTACTTTTTA CACCTCTGTC AAGAACTCCT
            661 GGACAAACCT CTGACCTGTG TGGAACAGAG TGGATATGGG TGTCTGAACA GATATTCACG
            721 TCTTTTGCAG ATCAGAGGGC ATTTCTGGTG TTGTGCAATG ACCTGGGTCC AACAGGTAGG
            781 CGTACTCGTA TCCAAGAGCA TTGTTGGACA GGAGGTAGTC TCCATTCCTA TAAAGAGGAA
            841 GGAATGGCAC CATGTAGTAG CCGTCATTGT GGCCAATTGG TGCATTGGTA CGTGGGTAGA
            901 TGGACCGGGG AGGCTGATGA GTTCTTAGCC AGCGCTCAAA GATGCTAGAA AATGATGTAA
            961 GAAACAAATA TGCAAGATGA AAAGTTAAGA TAAAGAATAA AAAAATTAAC AAATAAATGG
           1021 CAATTAATGG TTTTGTGTTC TAATTCCAAC CAATAATATC CTTCACGGCA GTAAAGGCCC
          1081 ACCAGGAATA TTTTTTGTTT CTTGCTCTGC AGGGGCCAAC CAAACTCCTT AAC
          //
          Primer: pAxEF_R2
          Region: Tol2 L
          Sequence file: RDB15278_A7H9d.seq check
          >D04498I9_A7H9_2_pAxEF_R2_D07_10_ABI24.ab1
              1 GGAGGTTTAA AATGGCCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA
             61 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC
            121 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
            181 ATTATGATCC TCTAGATCAG ATCTGCGAAG ATACGGCCAC GGGTGCTCTT GATCCTGTGG
            241 CTGATTTTGG ACTGTGCTGC TCGCAGCTGC TGATGAATCA CATACTTCCT CCATTTTCTT
            301 CCACTGATTG ACTGTTATAA TTTCCCTAAT TTCCAGGTCA AGGTGCTGTG CATTGTGGTA
            361 ATAGATGTGA CATGACGTCA CTTCCAAAGG ACCAATGAAC ATGTCTGACC AATTTCATAT
            421 AATGTGAAAA CGATTTTCAT AGGCAGAATA AATAACATTT AAATTAAACT GGGCATCAGC
            481 GCAATTCAAT TGGTTTGGTA ATAGCAAGGG AAAATAGAAT GAAGTGATCT CCAAAAAATA
            541 AGTACTTTTT GACTGTAAAT AAAATTGTAA GGAGTAAAAA GTACTTTTTT TTCTAAAAAA
            601 ATGTAATTAA GTAAAAGTAA AAGTATTGAT TTTTAATTGT ACTCAAGTAA AGTAAAAATC
            661 CCCAAAAATA ATACTTAAGT ACAGTAATCA AGTAAAATTA CTCAAGTACT TTACACCTCT
            721 GGTTCTTGAC CCCCTACCTT CAGCAAGCCC AGCAGATCCA CTAGTTCTAG AGCGGCCGCC
            781 ACCGCGGTGG AGCTCCAGCT TTTGTTCCCT TTAGTGAGGG TTAATTGCGC GCTTGGCGTA
            841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
            901 ACGAGCCGGA AGCATAAAAG TGTAAAGGCC TGGGGTGCCC TAATGAAGTG AGCTAACTCA
            961 CATTAAATTG CGTTGCGCTC ACTGCCCGCT TTTCCAGTCG GGAAACCCTG TCGTTGGCCA
           1021 GCTGGCATTA AATGAAATCG GGCCAACCGC GCGGGGGAAG AAGGGCGGTT TTGGCGTATT
           1081 TGGGCCGCTC CTTCGGCCTT TCCATCGCTC ACTGGACTTC GCTTGCGGCT TCGGGTTCGT
          1141 TTTCGGACCT TGGCGGCGGA AGAGCGGGT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Fukuhara, S., Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev. Biol. 393 (1): 10-23 (2014). PMID 24975012.
          original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430.
          reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl