RDB15134 - YC2.60/pRSETB

Catalog number	RDB15134
Resource name	YC2.60/pRSETB
Clone info.	Calcium ion sensor clone YC2.60 Yellow cameleon having wild-type CaM and the circular permutated cp173Venus. Bacterial expression plasmid. Known differences (at least): C to T at 291nt (97aa, Arg, synonym), T to C at 438nt (146aa, Tyr, synonym), A to C at 493nt (165aa, Asn to His), G to C at 639nt (213aa, Lys to Asn) of AB178711.1. Nucleotide sequence of AB178711.1 will be updated.
Vector backbone	pRSETB (plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Amp^r
Gene/insert name	X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence	AB178711 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Other clones in our bank	X. laevis (KEGG orthology K02183) \| synthetic (KEGG orthology K00907) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki

Terms and conditions set forth by the DEPOSITOR

1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

Ordering

Please visit Information of Request for Distribution.[link]
Order form [Credit Card Payment

] [Bank Transfer or Check Payment

]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word

]
Approval form [Word]

by Dr. Atsushi Miyawaki

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15134	YC2.60/pRSETB	DNA solution

original	Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

Featured content	Calcium Ion Sensor Clones (English text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: T7_20bp Sequence file: RDB15134_A7H9a.seq
>15134_15134_A7H9_1_T7_20bp_A08_02_ABI24.ab1 1 GAAAGTTCCA GCTAGGATAA TTTTGTTTAC TTTAAGAAGG AGATATAACA TATGCGGGGT 61 TCTCATCATC ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG 121 GATCTGTACG ACGATGACGA TAAGGATCCC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC 181 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAG GTTCAGCGTG 241 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC 301 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTG GGGCGTGCAG 361 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC 421 GAAGGCTACG TCCAGGAGCG TACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC 481 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC 541 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAT CAGCCACAAC 601 GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCCACTTCAA GATCCGCCAC 661 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC 721 GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA 781 GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCCGCATG 841 CATGACCAAC TGACAGAGAG CAGATTGCAG AGTCAAAGAG CCTTCTCATT ATTCGACACG 901 ATGGGGACGG CACCATCACA CAAGGACTGG CACCGTATGA AGTCGCTGAC AAACCCACGA 961 GCAGATGCAG ATATGATCAA TGAGTCGATG CTGATGCAAT GACCGATACC TGCTGACTCT 1021 ACTATGGATG GCTAGAATGA GACCAGACCG CGAAAGATCC GAAGCATTCC GTTTGACAGA 1081 TGAACGACTA CTCAGGCCTG CGTATACGTC ACGTCTG //
Primer: f1ori_R3 Sequence file: RDB15134_A7H9b.seq
>15134_15134_A7H9_1_f1ori_R3_C08_08_ABI24.ab1 1 GATCCTTACT CTTTTTTCAT TAGCTGCGCA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC 61 TTCGCTATTA CGCCAGATCC GGATATAGTT CCTCCTTTCA GCAAAAAACC CCTCAAGACC 121 CGTTTAGAGG CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA 181 GCTTCCTTTC GGGCTTTGTT AGCAGCCGGA TCAAGCTTCG AATTCTTACT CGATGTTGTG 241 GCGGATCTTG AAGTTGGCCT TGATGCCGTT CTTCTGCTTG TCGGCGGTGA TATAGACGTT 301 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC 361 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG 421 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG 481 CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTG 541 CAGGCCGTAG CCCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT 601 GCAGATCAGC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC 661 GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT 721 GAACAGCTCC TCGCCCTTGC TCACCATGCC ACCGCTGCCA CCCTTGTACA GCTCGTCCAT 781 GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC 841 GTTGGGGTCT TTGCTCAGGG CGGACTGGTA GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC 901 GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCCGCCGTC 961 CATGAGCTCC AGTGCCCCGG GAGCTGGAGA TCTTCTTGAA CCGGGTTGGC AGCGCTGACG 1021 GCAATGAAGT TTTTCTTCCA GCGCCTCTTC CCCCCCCTTG CTGTCATCAT TGTACAAACT 1081 CTTTCATAGT TACTGGGCAT CAACATCGAA TATCTGCTCC CTTATCAATT TCAATCCAAC 1141 CTTCCTTCCA TCCTGATTTA AACTTTCCTT CCCCCCGGAG GGATTTGTGT TTCAAATGAT 1201 GAC //