Yellow cameleon calcium ion sensor clone YC2.60.
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External Database X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() Approval form [Word] ![]() |
Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
提供条件 | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() 宮脇敦史博士による提供承諾書 [Word ![]() |
備考 | 提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15134 | YC2.60/pRSETB | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Calcium Ion Sensor Clones (English text) |
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RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15134_A7H9p1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: T7_20bp Region: 6xHis,T7 tag,X press tag,insert 5' Sequence file: RDB15134_A7H9a.seq ![]() |
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>15134_15134_B2CuF_T7_20bp_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNTTGT TNNNCTTTAA GAAGGAGNNA TACATATGCG GGGTTCTCAT 61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG 121 TACGACGATG ACGATAAGGA TCCCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG 181 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAGGTTCAG CGTGTCCGGC 241 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC 301 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTGGGGCGT GCAGTGCTTC 361 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC 421 TACGTCCAGG AGCGTACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG 481 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG 541 GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACATCAGCCA CAACGTCTAT 601 ATCACCGCCG ACNAGCAGAA GANCGGCATC NAGGCCCACT TCNAGANCCG CCACAACATC 661 NAGGNNGGCA GCGNGCAGCT CGNNGANCAC TACCAGCAGA ANNCCCCNNT CNGNNNNNGN 721 CCCNNNCTNC NNNCNNNNNN NNNN // |
Primer: T7ter_R1 Region: insert 3' Sequence file: RDB15134_A7H9b.seq ![]() |
>15134_15134_B2CuF_T7ter_R1_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNCNN NNTNNTTGCT NNGCGGTGGC AGCAGCCAAC TCAGCTTCCT 61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACTCGATGTT GTGGCGGATC 121 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG 181 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC 241 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG 301 TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG 361 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG 421 TAGCCCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC 481 AGCTTCAGGG TCAGCTTGCC GTAGGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC 541 TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACCAGGATGG GCACCACCCC GGTGAACAGC 601 TCCTCGCCCT TGCTCACCAT GCCACCGCTG CNACCCTTGT ACAGCTCGTC CNTGCCGAGA 661 GTGATCCCNG CGGCGGTCAC NAACTCCAGC NNNNCATGNG ATCGCGCTTC NCNNTNGGGN 721 NCTTNNNTCN NNNNNGNNNN NNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2022.05.17
GNP_filter3_RDBDEP_html_220516.pl