Resource data sheet
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YC2.60/pRSETB

Yellow cameleon calcium ion sensor clone YC2.60.

Catalog number RDB15134
Resource name YC2.60/pRSETB
Clone info. Calcium ion sensor clone YC2.60 Yellow cameleon having wild-type CaM and the circular permutated cp173Venus. Bacterial expression plasmid.
Known differences (at least): C to T at 291nt (97aa, Arg, synonym), T to C at 438nt (146aa, Tyr, synonym), A to C at 493nt (165aa, Asn to His), G to C at 639nt (213aa, Lys to Asn) of AB178711.1. Nucleotide sequence of AB178711.1 will be updated.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AB178711 (DDBJ accession number)
Depositor Miyawaki, Atsushi |
 
Other clones in our bank X. laevis (KEGG orthology K02183) | synthetic (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15134 YC2.60/pRSETB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Calcium Ion Sensor Clones (English text)

References

original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15134_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15134_A7H9a.seq
>15134_15134_A7H9_1_T7_20bp_A08_02_ABI24.ab1
    1 GAAAGTTCCA GCTAGGATAA TTTTGTTTAC TTTAAGAAGG AGATATAACA TATGCGGGGT
   61 TCTCATCATC ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG
  121 GATCTGTACG ACGATGACGA TAAGGATCCC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC
  181 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAG GTTCAGCGTG
  241 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
  301 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTG GGGCGTGCAG
  361 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC
  421 GAAGGCTACG TCCAGGAGCG TACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC
  481 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC
  541 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAT CAGCCACAAC
  601 GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCCACTTCAA GATCCGCCAC
  661 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC
  721 GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA
  781 GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCCGCATG
  841 CATGACCAAC TGACAGAGAG CAGATTGCAG AGTCAAAGAG CCTTCTCATT ATTCGACACG
  901 ATGGGGACGG CACCATCACA CAAGGACTGG CACCGTATGA AGTCGCTGAC AAACCCACGA
  961 GCAGATGCAG ATATGATCAA TGAGTCGATG CTGATGCAAT GACCGATACC TGCTGACTCT
 1021 ACTATGGATG GCTAGAATGA GACCAGACCG CGAAAGATCC GAAGCATTCC GTTTGACAGA
1081 TGAACGACTA CTCAGGCCTG CGTATACGTC ACGTCTG
//
Primer: f1ori_R3 RDB15134_A7H9b.seq
>15134_15134_A7H9_1_f1ori_R3_C08_08_ABI24.ab1
    1 GATCCTTACT CTTTTTTCAT TAGCTGCGCA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC
   61 TTCGCTATTA CGCCAGATCC GGATATAGTT CCTCCTTTCA GCAAAAAACC CCTCAAGACC
  121 CGTTTAGAGG CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA
  181 GCTTCCTTTC GGGCTTTGTT AGCAGCCGGA TCAAGCTTCG AATTCTTACT CGATGTTGTG
  241 GCGGATCTTG AAGTTGGCCT TGATGCCGTT CTTCTGCTTG TCGGCGGTGA TATAGACGTT
  301 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC
  361 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG
  421 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG
  481 CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTG
  541 CAGGCCGTAG CCCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT
  601 GCAGATCAGC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC
  661 GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT
  721 GAACAGCTCC TCGCCCTTGC TCACCATGCC ACCGCTGCCA CCCTTGTACA GCTCGTCCAT
  781 GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC
  841 GTTGGGGTCT TTGCTCAGGG CGGACTGGTA GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC
  901 GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCCGCCGTC
  961 CATGAGCTCC AGTGCCCCGG GAGCTGGAGA TCTTCTTGAA CCGGGTTGGC AGCGCTGACG
 1021 GCAATGAAGT TTTTCTTCCA GCGCCTCTTC CCCCCCCTTG CTGTCATCAT TGTACAAACT
 1081 CTTTCATAGT TACTGGGCAT CAACATCGAA TATCTGCTCC CTTATCAATT TCAATCCAAC
 1141 CTTCCTTCCA TCCTGATTTA AACTTTCCTT CCCCCCGGAG GGATTTGTGT TTCAAATGAT
1201 GAC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

2019.03.18

GNP_filter3_RDBDEP_html_190316.pl