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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

YC2.60/pRSETB

Yellow cameleon calcium ion sensor clone YC2.60.

Catalog number RDB15134
Resource name YC2.60/pRSETB
Clone info. Calcium ion sensor clone YC2.60 Yellow cameleon having wild-type CaM and the circular permutated cp173Venus. Bacterial expression plasmid.
Comment Known differences (at least): C to T at 291nt (97aa, Arg, synonym), T to C at 438nt (146aa, Tyr, synonym), A to C at 493nt (165aa, Asn to His), G to C at 639nt (213aa, Lys to Asn) of AB178711.1. Nucleotide sequence of AB178711.1 will be updated.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AB178711 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
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External Database
X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
        5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
        5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件は英文のまま記載してください。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15134 YC2.60/pRSETB DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15134_A7H9p1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: T7_20bp
        Region: 6xHis,T7 tag,X press tag,insert 5'
        Sequence file: RDB15134_A7H9a.seq check
        >15134_15134_B2CuF_T7_20bp_A01_01_ABI08.ab1
            1 NNNNNNNNNN NNNNNNTTGT TNNNCTTTAA GAAGGAGNNA TACATATGCG GGGTTCTCAT
           61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG
          121 TACGACGATG ACGATAAGGA TCCCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
          181 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAGGTTCAG CGTGTCCGGC
          241 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC
          301 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTGGGGCGT GCAGTGCTTC
          361 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC
          421 TACGTCCAGG AGCGTACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
          481 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG
          541 GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACATCAGCCA CAACGTCTAT
          601 ATCACCGCCG ACNAGCAGAA GANCGGCATC NAGGCCCACT TCNAGANCCG CCACAACATC
          661 NAGGNNGGCA GCGNGCAGCT CGNNGANCAC TACCAGCAGA ANNCCCCNNT CNGNNNNNGN
        721 CCCNNNCTNC NNNCNNNNNN NNNN
        //
        Primer: T7ter_R1
        Region: insert 3'
        Sequence file: RDB15134_A7H9b.seq check
        >15134_15134_B2CuF_T7ter_R1_B01_02_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNCNN NNTNNTTGCT NNGCGGTGGC AGCAGCCAAC TCAGCTTCCT
           61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACTCGATGTT GTGGCGGATC
          121 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG
          181 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC
          241 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG
          301 TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG
          361 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG
          421 TAGCCCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC
          481 AGCTTCAGGG TCAGCTTGCC GTAGGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC
          541 TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACCAGGATGG GCACCACCCC GGTGAACAGC
          601 TCCTCGCCCT TGCTCACCAT GCCACCGCTG CNACCCTTGT ACAGCTCGTC CNTGCCGAGA
          661 GTGATCCCNG CGGCGGTCAC NAACTCCAGC NNNNCATGNG ATCGCGCTTC NCNNTNGGGN
        721 NCTTNNNTCN NNNNNGNNNN NNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl