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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

GEPRA-AA/ pT2KXIGdeltain (#RDB15254)

Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish. Low-affinity verson of the GEPRA-B.

Clone info. Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish. Low-affinity verson of the GEPRA-B, having ligand binding domain from the RAR-beta with two amino acid substitutions (R269A and S280A).
Vector backbone pT2KXIG deltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC
Gene/insert name mouse RARbeta cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S. et al., Nature 496 (7445): 363-366 (2013).
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S. et al., Nature 496 (7445): 363-366 (2013).
付加的使用条件：
Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15254 GEPRA-AA/ pT2KXIGdeltain DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The GEPRA-AA/ pT2KXIGdeltain was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15254).

Reference section:

Shimozono, S., Iimura, T., Kitaguchi, T., Higashijima, S., Miyawaki, A., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15254_A7H9p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F2
Sequence file: RDB15254_A7H9a.seq check
>D04498G3-1_A7H9_EF1a-C_F2_C11_08_ABI24.ab1
    1 GGTGGGGGTT AGGCGGTTCG CTCTCTTCCT CACCGGGTCT GCGGCGAGTT CTAGCTGAAG
   61 CTTCCTGCAG GTCGACCGAT CCTGAGAACT TCAGGGTGAG TTTGGGGACC CTTGATTGTT
  121 CTTTCTTTTT CGCTATTGTA AAATTCATGT TATATGGAGG GGGCAAAGTT TTCAGGGTGT
  181 TGTTTAGAAT GGGAAGATGT CCCTTGTATC ACCATGGACC CTCATGATAA TTTTGTTTCT
  241 TTCACTTTCT ACTCTGTTGA CAACCATTGT CTCCTCTTAT TTTCTTTTCA TTTTCTGTAA
  301 CTTTTTCGTT AAACTTTAGC TTGCATTTGT AACGAATTTT TAAATTCACT TTTGTTTATT
  361 TGTCAGATTG TAAGTACTTT CTCTAATCAC TTTTTTTTCA AGGCAATCAG GGTATATTAT
  421 ATTGTACTTC AGCACAGTTT TAGAGAACAA TTGTTATAAT TAAATGATAA GGTAGAATAT
  481 TTCTGCATAT AAATTCTGGC TGGCGTGGAA ATATTCTTAT TGGTAGAAAC AACTACACCC
  541 TGGTCATCAT CCTGCCTTTC TCTTTATGGT TACAATGATA TACACTGTTT GAGATGAGGA
  601 TAAAATACTC TGAGTCCAAA CCGGGCCCCT CTGCTAACCA TGTTCATGCC TTCTTCTCTT
  661 TCCTACAGCT CCTGGGCAAC GTGCTGGTTG TTGTGCTGTC TCATCATTTT GGCAAAGAAT
  721 TCCTCGACGG ATCCCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
  781 CTGGTCGAGC TGCAGGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG
  841 GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
  901 GTGCCCTGGC CCACCCTCGT GACCACCCTG AGCTGGGGCG TGCAGTGCTT CAGCCGCTAC
  961 CCCGACCACA TGAAAGCAGC ACGACTTTCT TTCAAGTCCC GTCATGCCCG AAGCTACGTC
 1021 CAGGAGCGCA CCCATCCTCC TTCAAGGACG ACCGCACTAC CAGGACCGCG CGAGTTGAGG
1081 TTCGAGGCGA CACCTGGTGA CCGCATCGAG CCTGGATGGT CAATTCGAAC CTTCCA
//
Primer: pAxCALNL_F1
Sequence file: RDB15254_A7H9b.seq check
>D04498G3-1_A7H9_pAxCALNL_F1_D11_11_ABI24.ab1
    1 ATTATCTATC TTATCATGTC TGGATCATCA TCGATGAATT AATTCGAGCT CCACCGCGGT
   61 GGCGGCCGCT CTAGATGCAT GCTCGAGTTA CTTGTACAGC TCGTTCATGC CTTCAGTGAT
  121 CCCGGCGGCG GTCAGGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT
  181 GAACAGGGCG GACTGGTAGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC
  241 GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CCGCCGTCCT CGATGTTGTG
  301 GCGGATCTTG AAGTTGGCCT TGATGCCGTT CTTCTGCTTG TCGGCGGTGA TATAGACGTT
  361 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC
  421 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG
  481 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG
  541 CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTG
  601 CAGGCCGTAG CCCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT
  661 GCAGAGCAGC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC
  721 GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT
  781 GAACAGCTCC TCGCCCTTGC TCACCATGAG CTCTTCATGT CCTTCAGAAT TCTCCAGCAT
  841 TTCCTGAATG AGAGGTGGCA TTGATCCAGG AATTTCCATT TTCAAAGGTA ATTACACGTT
  901 CGGCACCTTT CGCGCTGATG CTGCGGAGAT CTGTGATTTT CATTAAGATC TTTGGAAACA
  961 TGTGAGGCTT TGCTGGGTCG TCGTTTTCTA ATGTAAATCT TTAGTGCTTT CCAGCAGTGG
 1021 TTCTTCTAGC TTGTCTACTT TTGATGGTTT CCTCACGTCT GACCGGGTCT CAACAGATCA
1081 AACAGATTGC AACTGAGACG CCTTGCTTGT GTGGGTGATC CAATC
//
Primer: RARbeta_LBD
Sequence file: RDB15254_A7H9c.seq check
>D04498G3_1_A7H9_RARbeta_LBD_H01_08_ABI08.ab1
    1 CGGTAGGAAA AAGGTGGCAT TGATCCAGGA ATTTCCATTT TCAAGGTAAT TACACGTTCG
   61 GCACCTTTCG CGCTGATGCT GCGGAGATCT GTGATTTTCA TTAAGATCTT TGGAAACATG
  121 TGAGGCTTGC TGGGTCGTCG TTTTCTAATG TAAATCTTTA GTGCTTCCAG CAGTGGTTCT
  181 TGGAGCTTGT CTACTTTTGT TGGTTCCTCA AGGTCCTGGC GGTCTCCACA GATTAAACAG
  241 ATGGCACTGA GAAGGCCTGT TTCTGTGTCA TCCATTTCCA AAGGCAGGAG CTGGTTGGCA
  301 AAGGTGAACA CAAGGTCAGT CAGAGGACCG AAGCCAGCAT TGTGCATCTG AGTTCGATTT
  361 AGTGTAAGGC CATCAGCGAA AGTCATGGTG TCTTGCTCTG GGGTATAGGC GGTACAAATT
  421 CTGAGAATCA AGATATCCAA GCAGGCGGCT TTGAGCAGGG TGATCTGGTC TGCGATGGTC
  481 AGACCTGTGA AGCCCGGCAG ACGCTTGGCG AACTCCACGA TCTTAATAAT GCACTTGGTG
  541 GCCAGCTCAC TGAATTTGTC CCAGAGGCCC AAGTCCAATC GGACCCGGTG GTCAGCGCTG
  601 GAATTCGTGG TGTATTTACC CAGCTGGCAG AGTGAGGGAA AGGTTTCCTG GTGGGCTTTC
  661 CGGATCTTCT CAGTGAGGTC GTCTAGCTCC GCTGTCATCT CATAGCTCTC ATGCATGCGG
  721 GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC
  781 AGGGCGGACT GGGTGCTCAG GTAGTGGTTG TCGGGCAGGT TCACGGGGCC GTCGCCGATG
  841 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCACGAT GTTGTGGCGG
  901 ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG
  961 CTGATGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CCGTCCTCCC TTGAAGTCGA
 1021 TGCCCTTCAG CTCGAATGCG GTTCACAGGG TGTCGCCTCG AACTTTCACT CGGCGCGGGT
 1081 CCTGTAGGTG CGGTCGTCCT GGAGGAGATT GGGTGCGCCT CCTGGACGTA GCCCTTTCGG
1141 GGCATGGAAC G
//
Primer: EF1a-N_R2
Sequence file: RDB15254_A7H9d.seq check
>D04498G3-1_A7H9_EF1a-N_R2_E11_14_ABI24.ab1
    1 TTACGGGCAA GATTGCAGTT TGATTTGCAT TGGAAAGGGT CGCTGGCTTT TGTGTTACAC
   61 GCCCCTTATT TGTGCTTGAT TAGATGATCC CCCTGCTCGA GGGCCCATCT GGCCTGTGTT
  121 TCAGACACCA GGGAGTCTCT GCTCACGTTT CCTGCTATTT GCAGCCTCTC TATCAAGACT
  181 AATACACCTC TTCCCGCATC GGCTGCCTGT GAGAGGCTTT TCAGCACTGC AGGATTGCTT
  241 TTCAGCCCCA AAAGAGCTAG GCTTGACACT AACAATTTTG AGAATCAGCT TCTACTGAAG
  301 TTAAATCTGA GGTTTTACAA CTTTGAGTAG CGTGTACTGG CATTAGATTG TCTGTCTTAT
  361 AGTTTGATAA TTAAATACAA ACAGTTCTAA AGCAGGATAA AACCTTGTAT GCATTTCATT
  421 TAATGTTTTT TGAGATTAAA AGCTTAAACA AGAATCTCTA GTTTTCTTTC TTGCTTTTAC
  481 TTTTACTTCC TTAATACTCA AGTACAATTT TAATGGAGTA CTTTTTTACT TTTACTCAAG
  541 TAAGATTCTA GCCAGATACT TTTACTTTTA ATTGAGTAAA ATTTTCCCTA AGTACTTGTA
  601 CTTTCACTTG AGTAAAATTT TTGAGTACTT TTTACACCTC TGTCAAGAAC TCCTGGACAA
  661 ACCTCTGACC TGTGTGGAAC AGAGTGGATA TGGGTGTCTG AACAGATATT CACGTCTTTT
  721 GCAGATCAGA GGGCATTTCT GGTGTTGTGC AATGACCTGG GTCCAACAGG TAGGCGTACT
  781 CGTATCCAAG AGCATTGTTG GACAGGAGGT AGTCTCCATT CCTATAAAGA GGAAGGAATG
  841 GCACCATGTA GTAGCCGTCA TTGTGGCCAA TTGGTGCATT GGTACGTGGG TAGATGGACC
  901 GGGGAGGCTG ATGAGTTCTT AGCCAGCGCT CAAGATGCTA GAAATGATGT AAGAAACAAA
  961 TATGCAGATG AAAGTTTAAG ATAAGATAAA AAATTAAAAC AATAAATGCA ATTATGTTTT
 1021 GTGTTTCTAT CCACCAATTA TTATCTCCAC GCAGTAAAGC CACCAAGAAT ATTTTTTGGT
1081 CCTTGCTCTG CAGCAAGTCA CCTTACATTG AATCTCAACT GTGA
//
Primer: pT2KXIG#1
Sequence file: RDB15254_A7H9e.seq check
>D04498G3_1_A7H9_pT2KXIG#1_G01_07_ABI08.ab1
    1 AGGCCGCCAA TTAACCTCAC TAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC
   61 GCTCTAGAAC TAGTGGATCT GCTGGGCTTG CTGAAGGTAG GGGGTCAAGA ACCAGAGGTG
  121 TAAAGTACTT GAGTAATTTT ACTTGATTAC TGTACTTAAG TATTATTTTT GGGGATTTTT
  181 ACTTTACTTG AGTACAATTA AAAATCAATA CTTTTACTTT TACTTAATTA CATTTTTTTA
  241 GAAAAAAAAG TACTTTTTAC TCCTTACAAT TTTATTTACA GTCAAAAAGT ACTTATTTTT
  301 TGGAGATCAC TTCATTCTAT TTTCCCTTGC TATTACCAAA CCAATTGAAT TGCGCTGATG
  361 CCCAGTTTAA TTTAAATGTT ATTTATTCTG CCTATGAAAA TCGTTTTCAC ATTATATGAA
  421 ATTGGTCAGA CATGTTCATT GGTCCTTTGG AAGTGACGTC ATGTCACATC TATTACCACA
  481 ATGCACAGCA CCTTGACCTG GAAATTAGGG AAATTATAAC AGTCAATCAG TGGAAGAAAA
  541 TGGAGGAAGT ATGTGATTCA TCAGCAGCTG CGAGCAGCAC AGTCCAAAAT CAGCCACAGG
  601 ATCAAGAGCA CCCGTGGCCG TATCTTCGCA GATCTGATCT AGAGGATCAT AATCAGCCAT
  661 ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG
  721 AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC
  781 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT
  841 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCATCAT CGATGAATTA
  901 ATTCGAGCTC CACCGCGGTG GCGGCCGCTC TAGATGCATG CTCGAGTTAC TTGTACAGCT
  961 CGTTCATGCC CTTCAGTGAT CCCGGCGGCG GTCAGGAACT CCAGCAGGAC CATGTGATCG
 1021 CGCTTCTCGT TGGGGTCTTT TGAACAGGCG GACTGGTAGC TCAGTAGTGG GTTGTCGGCA
 1081 AGCAGCACGG TGGTCGCTGA TGGGGGTGTT CTGCTGGTAG TGATCCGCCG AGCCTGCACG
1141 GCCGGCCGAT CCCTTCGCGA ATGGTTTGTT GTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. [link to RRC of NBRP]
review Schilling, T.F., Visualizing retinoic acid morphogen gradients. Methods Cell Biol. 133: 139-163 (2016). PMID 27263412.