Please review the QC test results indicated by icon below as well as clone information before placing your order.

mCherry-hCdt1(30/120)/pcDNA3

Expression vector of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Ordering	Please visit Information of Request for Distribution.[link] UCSD's MTA is also required. Please visit Additional Forms section. Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	UCSD's MTA is also required. Please visit Additional Forms section.

Distribution information (domestic users) [open/close]

提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。別途、UCSDとのMTAも必要です。「申込みに必要な書式」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	提供条件は英文のまま記載してください。 別途、UCSDとのMTAも必要です。「申込みに必要な書式」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15457_A7Jvp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB15457_A7Jva.seq
>D05018B8-2_A7Jv_CMV-Forward_C03_09_ABI24.ab1     1 AGTTCAACTC CGGCCCCCAA TTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC    61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT   121 AATACGACTC ACTATAGGGA GACCCAAGCT TGGTACCGAG CTCGGATCCA CTAGTAACGG   181 CCGCCAGTGT GCTGGAATTC GCCACCATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA   241 TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT   301 TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA   361 AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT   421 ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT   481 TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG   541 TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA   601 CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT   661 CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC   721 TGAAGGACGG CGGCCACTAC GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG   781 TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG   841 ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG   901 ACGAGCTGTA CAAGGGATAT CCATCACACT GGCGGGCCGC TCGAGCCCAG CCCCGCCAGG   961 CCCCGCACTC CGCGCCCCGG GCCTCCGCTA CCAGTGGCAG CCGCAAGCGC CGCCCCGCCC  1021 GCCCCGCCGC CCCCGGGACG CGACCAGTCA GTCACCGGGC CGCAAGAGAC TGCCGCTGTC  1081 CGTTGAACGA GTTTCAGTCC TCAGTACCCC GGAAGGTCCT CAGAACATTC CTCAACCCTG 1141 GTCCC //
Primer: BGH_rev2 Sequence file: RDB15457_A7Jvb.seq
>D05018B8-2_A7Jv_BGH_rev2_D03_12_ABI24.ab1     1 CATGGGGGTA GAAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG    61 TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT   121 TAGATGGTGT CCTGGTCCTG CGCGGATGTC AGGTGGGGCG GCTGACCTGC TGCCGGGGTG   181 GATTTCTTTA TCTTCTGGCC CGGAGAAGGG CAGGCTGGGA TGTCTGGGGC CTCGGGGGTA   241 CTGGGGCTGG AAACCTCGTC CACCGACAGC CGCAGTCTCC TGCGGGCCGG TGGCCTGGCC   301 TGGTCGCGTC CGGGGGCGGC GGGCGGGCGG GCGCGCTTGC GGCTGCCACT GGTAGCGGAG   361 GCCGGGGCGC GGAGTGCGGG CCTGGCGGGG CTGGGCTCGA GCGGCCGCCA GTGTGATGGA   421 TATCCCTTGT ACAGCTCGTC CATGCCGCCG GTGGAGTGGC GGCCCTCGGC GCGTTCGTAC   481 TGTTCCACGA TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAACTTGAT GTTGACGTTG   541 TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG   601 TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC TGCTTGATCT CGCCCTTCAG GGCGCCGTCC   661 TCGGGGTACA TCCGCTCGGA GGAGGCCTCC CAGCCCATGG TCTTCTTCTG CATTACGGGG   721 CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC TTCACCTTGT AGATGAACTC GCCGTCCTGC   781 AGGGAGGAGT CCTGGGTCAC GGTCACCACG CCGCCGTCCT CGAAGTTCAT CACGCGCTCC   841 ACTTGAAGCC CTCGGGGAAG GACAGCTTCA AGTAGTCGGG GATGTCGGCG GGGTGCTTCA   901 CGTAGGCCTT GGAGCCGTAC ATGAACTGAG GGGACAGGAT GTCCCAGGCG AAAGGGCAGG   961 GGGCCACCCT TGGGTCACCT TTCAGCTTGG CGGTCTGGGT GCCCTCGTAG GGCGGGCCCT  1021 CGCCCCTCGC CTCGATCTCG AACTCGTGGC CGTTCACGAG GCCTCATGTG CACCTTGGAG 1081 CGCATGACTC ATGATGAATG ACATGCTATC CCTTCCTTCG GTGCT //
Primer: SV40pro_F Sequence file: RDB15457_A7Jvc.seq
>D05018B8_2_A7Jv_SV40pro_F_C07_07_ABI24.ab1     1 ACTGGTCCTG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA    61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG   121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG   661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT   781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC   841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC   901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG   961 CCCAACCTGC CATCACGAGA TTTCGATTCC CACCGCCCGC CCTTCTATGA AAGGTTGGGG  1021 CTTTCGGAAT CGTTTTCCCG GACGCCGGGC TGGGATGATC CCTCCAGCCG CGGGGAATCT  1081 TCAATGCTGC AGTTCATCAC CCACTCGACT TTGGTTAATT GCAGCTTATA ATGGTTACCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.05.18

GNP_filter3_RDBDEP_html_210503.pl