RDB15457 - mCherry-hCdt1(30/120)/pcDNA3

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

mCherry-hCdt1(30/120)/pcDNA3 (#RDB15457)

Expression vector of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe.

Clone info.	Expression vector of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe. Fluorescent was detected in the nucleus.
Vector backbone	pcDNA3 (plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name	Human CDT1 cDNA Discosoma sp. RFP cDNA
Reference of insert sequence	AB512478 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.

Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
備考	提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15457	mCherry-hCdt1(30/120)/pcDNA3	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mCherry-hCdt1(30/120)/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15457).

Reference section:

Sakaue-Sawano, A., Kobayashi, T., Ohtawa, K., Miyawaki, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15457_A7Jvp1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB15457_A7Jvd.seq
>15457_15457_B3E2F_CMV_Forward_H01_08_ABI08.ab1 1 NNNNNNNNNN NNNCCCNNTT GNNGCAANNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT 61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT 121 ACGACTCACT ATAGGGAGAC CCAAGCTTGG TACCGAGCTC GGATCCACTA GTAACGGCCG 181 CCAGTGTGCT GGAATTCGCC ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA 241 TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG 301 AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG 361 TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG 421 GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC 481 CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA 541 CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA 601 ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG 661 AGCGGATGTA CCCCGAGGAC GGCGCCCTGA ANGGCGAGAT CAAGCAGANG CTGAAGCTGA 721 ANGACGGCGG CCACTACGAC GCTGANGTCA AGANCACCTA CAAGGCCNAG AAGCCCGTGC 781 AGCTGCCCGG CGCCTACNAC GTCNACATCA AGTTGGACNT CACCTCNNAC AACGANGNNT 841 ACACCATCNN NNNANNAGTA CGAACGCNNN NANGGNCGNN NCTCCNCCGG CGGNNTGGNN 901 GANCTGTNNN AGGGATATNN NTCACACTGG CNGCNGNNNN NNCCCANNNN CNNNNNGNNC 961 NNNANTNNNN NNNNNN //
Primer: BGH_rev2 (Pr0606) Region: insert 3' Sequence file: RDB15457_A7Jve.seq
>D05018B8-2_A7Jv_BGH_rev2_D03_12_ABI24.ab1 1 NNNNNNNNNN GNNNNNNGGG GGNNGGNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCANNNNGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT 121 TAGATGGTGT CCTGGTCCTG CGCGGATGTC AGGTGGGGCG GCTGACCTGC TGCCGGGGTG 181 GATTTCTTTA TCTTCTGGCC CGGAGAAGGG CAGGCTGGGA TGTCTGGGGC CTCGGGGGTA 241 CTGGGGCTGG AAACCTCGTC CACCGACAGC CGCAGTCTCC TGCGGGCCGG TGGCCTGGCC 301 TGGTCGCGTC CGGGGGCGGC GGGCGGGCGG GCGCGCTTGC GGCTGCCACT GGTAGCGGAG 361 GCCGGGGCGC GGAGTGCGGG CCTGGCGGGG CTGGGCTCGA GCGGCCGCCA GTGTGATGGA 421 TATCCCTTGT ACAGCTCGTC CATGCCGCCG GTGGAGTGGC GGCCCTCGGC GCGTTCGTAC 481 TGTTCCACGA TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAACTTGAT GTTGACGTTG 541 TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG 601 TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC TGCTTGATCT CGCCCTTCAG GGCGCCGTCC 661 TCGGGGTACA TCCGCTCGGA NGANGCCTCC CAGCCCATGG TCTTCTTCTG CATTACGGGG 721 CCGTCGGANG GGAAGTTGGT GCCGCGCAGC TTCACCTTGT AGATGAACTC GCCGTCCTGC 781 ANGGANGANT CCTGGGTCAC GGTCACCACG CCGCCGTCCT CGAAGTNCAT CACGCGCNNN 841 NCTTGAAGCC CTCNGGGAAN GANAGCTTCA AGTANTCNGG GNNGTCGGCG GGGTGCTNNA 901 CGNNNNNCTN NGAGCNNNNC NTGAANNNNN GGGNNAGGNN GNNCNNNNNN NANGGGNNNN 961 GGNNNNNCNN NNGNNNNNNN TNNNNCNNNN NNGNNNNGNN NNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: NeoR Sequence file: RDB15457_A7Jvf.seq
>D05018B8_2_A7Jv_SV40pro_F_C07_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNA NNNNTGNNGN NGCTTTTTTG GANGCCTAGG CTTTTGCAAA 61 AAGCTCCCGG GNGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 661 CCCGACGGCG ANGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGNNCGGC TGGGTGTGGC GGANCGCTAT 781 CANGACATAG CGTTGGCTAC NCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 841 NGCTTCCNCG TGCTTTACGG NATCGCCGCT CCCNANNCGC AGCGCATCGC CTTCTATCGC 901 CTTCTTGACG ANTTCTTCTG ANCGGGNNNC TGGGGGTTCN NAATGANNNA NNNANCGACG 961 CCNNANNNGN NNNCNCNANN NTNNNANTNC NNNNNNCNNN CNNNNNNNNN ANNNNNNGGN 1021 NNTNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]

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Other resources in our bank

human CDT1

External Database
Human CDT1

Discosoma sp. RFP

Reference sequence