RDB15457 - mCherry-hCdt1(30/120)/pcDNA3

Expression vector of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe.

Distribution information

Catalog number	RDB15457
Resource name	mCherry-hCdt1(30/120)/pcDNA3
Clone info.	Expression vector of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe. Fluorescent was detected in the nucleus.
Vector backbone	pcDNA3 (plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name	Human CDT1 cDNA Discosoma sp. RFP cDNA
Reference of nucleotide sequence	AB512478 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Other clones in our bank	human CDT1 (NCBI Gene 81620) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Ordering	Please visit Information of Request for Distribution.[link] UCSD's MTA is also required. Please visit Additional Forms section. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	UCSD's MTA is also required. Please visit Additional Forms section.

Distribution information (domestic users)

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提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。別途、UCSDとのMTAも必要です。「申込みに必要な書式」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	提供条件は英文のまま記載してください。別途、UCSDとのMTAも必要です。「申込みに必要な書式」をご覧ください。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15457	mCherry-hCdt1(30/120)/pcDNA3	DNA solution

original	Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962.

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: CMV-Forward Sequence file: RDB15457_A7Jva.seq
>D05018B8-2_A7Jv_CMV-Forward_C03_09_ABI24.ab1 1 AGTTCAACTC CGGCCCCCAA TTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT 121 AATACGACTC ACTATAGGGA GACCCAAGCT TGGTACCGAG CTCGGATCCA CTAGTAACGG 181 CCGCCAGTGT GCTGGAATTC GCCACCATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA 241 TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT 301 TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA 361 AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT 421 ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT 481 TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG 541 TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA 601 CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT 661 CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC 721 TGAAGGACGG CGGCCACTAC GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG 781 TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG 841 ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG 901 ACGAGCTGTA CAAGGGATAT CCATCACACT GGCGGGCCGC TCGAGCCCAG CCCCGCCAGG 961 CCCCGCACTC CGCGCCCCGG GCCTCCGCTA CCAGTGGCAG CCGCAAGCGC CGCCCCGCCC 1021 GCCCCGCCGC CCCCGGGACG CGACCAGTCA GTCACCGGGC CGCAAGAGAC TGCCGCTGTC 1081 CGTTGAACGA GTTTCAGTCC TCAGTACCCC GGAAGGTCCT CAGAACATTC CTCAACCCTG 1141 GTCCC //
Primer: BGH_rev2 Sequence file: RDB15457_A7Jvb.seq
>D05018B8-2_A7Jv_BGH_rev2_D03_12_ABI24.ab1 1 CATGGGGGTA GAAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT 121 TAGATGGTGT CCTGGTCCTG CGCGGATGTC AGGTGGGGCG GCTGACCTGC TGCCGGGGTG 181 GATTTCTTTA TCTTCTGGCC CGGAGAAGGG CAGGCTGGGA TGTCTGGGGC CTCGGGGGTA 241 CTGGGGCTGG AAACCTCGTC CACCGACAGC CGCAGTCTCC TGCGGGCCGG TGGCCTGGCC 301 TGGTCGCGTC CGGGGGCGGC GGGCGGGCGG GCGCGCTTGC GGCTGCCACT GGTAGCGGAG 361 GCCGGGGCGC GGAGTGCGGG CCTGGCGGGG CTGGGCTCGA GCGGCCGCCA GTGTGATGGA 421 TATCCCTTGT ACAGCTCGTC CATGCCGCCG GTGGAGTGGC GGCCCTCGGC GCGTTCGTAC 481 TGTTCCACGA TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAACTTGAT GTTGACGTTG 541 TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG 601 TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC TGCTTGATCT CGCCCTTCAG GGCGCCGTCC 661 TCGGGGTACA TCCGCTCGGA GGAGGCCTCC CAGCCCATGG TCTTCTTCTG CATTACGGGG 721 CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC TTCACCTTGT AGATGAACTC GCCGTCCTGC 781 AGGGAGGAGT CCTGGGTCAC GGTCACCACG CCGCCGTCCT CGAAGTTCAT CACGCGCTCC 841 ACTTGAAGCC CTCGGGGAAG GACAGCTTCA AGTAGTCGGG GATGTCGGCG GGGTGCTTCA 901 CGTAGGCCTT GGAGCCGTAC ATGAACTGAG GGGACAGGAT GTCCCAGGCG AAAGGGCAGG 961 GGGCCACCCT TGGGTCACCT TTCAGCTTGG CGGTCTGGGT GCCCTCGTAG GGCGGGCCCT 1021 CGCCCCTCGC CTCGATCTCG AACTCGTGGC CGTTCACGAG GCCTCATGTG CACCTTGGAG 1081 CGCATGACTC ATGATGAATG ACATGCTATC CCTTCCTTCG GTGCT //
Primer: SV40pro_F Sequence file: RDB15457_A7Jvc.seq
>D05018B8_2_A7Jv_SV40pro_F_C07_07_ABI24.ab1 1 ACTGGTCCTG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG 961 CCCAACCTGC CATCACGAGA TTTCGATTCC CACCGCCCGC CCTTCTATGA AAGGTTGGGG 1021 CTTTCGGAAT CGTTTTCCCG GACGCCGGGC TGGGATGATC CCTCCAGCCG CGGGGAATCT 1081 TCAATGCTGC AGTTCATCAC CCACTCGACT TTGGTTAATT GCAGCTTATA ATGGTTACCA //