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mCherry-hCdt1(30/120)/pcDNA3

Expression vector of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe.

Catalog number RDB15457
Resource name mCherry-hCdt1(30/120)/pcDNA3
Clone info. Expression vector of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe. Fluorescent was detected in the nucleus.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Human CDT1 cDNA Discosoma sp. RFP cDNA
Reference of nucleotide sequence AB512478 (DDBJ accession number)
Depositor Miyawaki, Atsushi |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Ordering Please visit Information of Request for Distribution. Please contact us. UCSD's MTA is also required.
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Remarks
Please contact us. UCSD's MTA is also required.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15457 mCherry-hCdt1(30/120)/pcDNA3 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15457_A7Jvp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB15457_A7Jva.seq
>D05018B8-2_A7Jv_CMV-Forward_C03_09_ABI24.ab1
    1 AGTTCAACTC CGGCCCCCAA TTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT TGGTACCGAG CTCGGATCCA CTAGTAACGG
  181 CCGCCAGTGT GCTGGAATTC GCCACCATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA
  241 TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT
  301 TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA
  361 AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT
  421 ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT
  481 TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG
  541 TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA
  601 CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT
  661 CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC
  721 TGAAGGACGG CGGCCACTAC GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG
  781 TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG
  841 ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG
  901 ACGAGCTGTA CAAGGGATAT CCATCACACT GGCGGGCCGC TCGAGCCCAG CCCCGCCAGG
  961 CCCCGCACTC CGCGCCCCGG GCCTCCGCTA CCAGTGGCAG CCGCAAGCGC CGCCCCGCCC
 1021 GCCCCGCCGC CCCCGGGACG CGACCAGTCA GTCACCGGGC CGCAAGAGAC TGCCGCTGTC
 1081 CGTTGAACGA GTTTCAGTCC TCAGTACCCC GGAAGGTCCT CAGAACATTC CTCAACCCTG
1141 GTCCC
//
Primer: BGH_rev2 RDB15457_A7Jvb.seq
>D05018B8-2_A7Jv_BGH_rev2_D03_12_ABI24.ab1
    1 CATGGGGGTA GAAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT
  121 TAGATGGTGT CCTGGTCCTG CGCGGATGTC AGGTGGGGCG GCTGACCTGC TGCCGGGGTG
  181 GATTTCTTTA TCTTCTGGCC CGGAGAAGGG CAGGCTGGGA TGTCTGGGGC CTCGGGGGTA
  241 CTGGGGCTGG AAACCTCGTC CACCGACAGC CGCAGTCTCC TGCGGGCCGG TGGCCTGGCC
  301 TGGTCGCGTC CGGGGGCGGC GGGCGGGCGG GCGCGCTTGC GGCTGCCACT GGTAGCGGAG
  361 GCCGGGGCGC GGAGTGCGGG CCTGGCGGGG CTGGGCTCGA GCGGCCGCCA GTGTGATGGA
  421 TATCCCTTGT ACAGCTCGTC CATGCCGCCG GTGGAGTGGC GGCCCTCGGC GCGTTCGTAC
  481 TGTTCCACGA TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAACTTGAT GTTGACGTTG
  541 TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG
  601 TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC TGCTTGATCT CGCCCTTCAG GGCGCCGTCC
  661 TCGGGGTACA TCCGCTCGGA GGAGGCCTCC CAGCCCATGG TCTTCTTCTG CATTACGGGG
  721 CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC TTCACCTTGT AGATGAACTC GCCGTCCTGC
  781 AGGGAGGAGT CCTGGGTCAC GGTCACCACG CCGCCGTCCT CGAAGTTCAT CACGCGCTCC
  841 ACTTGAAGCC CTCGGGGAAG GACAGCTTCA AGTAGTCGGG GATGTCGGCG GGGTGCTTCA
  901 CGTAGGCCTT GGAGCCGTAC ATGAACTGAG GGGACAGGAT GTCCCAGGCG AAAGGGCAGG
  961 GGGCCACCCT TGGGTCACCT TTCAGCTTGG CGGTCTGGGT GCCCTCGTAG GGCGGGCCCT
 1021 CGCCCCTCGC CTCGATCTCG AACTCGTGGC CGTTCACGAG GCCTCATGTG CACCTTGGAG
1081 CGCATGACTC ATGATGAATG ACATGCTATC CCTTCCTTCG GTGCT
//
Primer: SV40pro_F RDB15457_A7Jvc.seq
>D05018B8_2_A7Jv_SV40pro_F_C07_07_ABI24.ab1
    1 ACTGGTCCTG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG
  961 CCCAACCTGC CATCACGAGA TTTCGATTCC CACCGCCCGC CCTTCTATGA AAGGTTGGGG
 1021 CTTTCGGAAT CGTTTTCCCG GACGCCGGGC TGGGATGATC CCTCCAGCCG CGGGGAATCT
 1081 TCAATGCTGC AGTTCATCAC CCACTCGACT TTGGTTAATT GCAGCTTATA ATGGTTACCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962.

2019.07.26

GNP_filter3_RDBDEP_html_190432.pl