Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAAV2-TRE-mito-SRAI-BGHpA

AAV2 expression vector of mito-SRAI (signal-retaining autophagy indicator) driven by the tetracyclin-responsive element promoter for quantitative visualizing mitophagy.

Sequence (full)	RDB18684zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sohn, J. et al., PLoS One, 12 (1): e0169611, 2017. Hioki, H. et al., Neurosci. Res., 63 (2): 149-154, 2009. Katayama et al. (2020). Cell 181, 1176–1187. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sohn, J. et al., PLoS One, 12 (1): e0169611, 2017. Hioki, H. et al., Neurosci. Res., 63 (2): 149-154, 2009. Katayama et al. (2020). Cell 181, 1176–1187. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18684_B1B5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N (Pr0027) Region: mito-SRAI 5' (2xCOX8 pre seq), tight TRE pro, attB2, AAV2 ITR Sequence file: RDB18684_B1B5a.seq
>D07612A1_B1B5_1_EGFP-N_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTCGCCCTT GCTNNNCNTG GNNCCCCCGA NCTTCCCCTC    61 CGGCGGCAAC GAATGGATCT TGGCGCGCGG CACTGGGAGC CGCCGGGCCG AGCCTGTCAA   121 GCCCCGCAGC AGCAGCGGCG TCAGGACGGA CATCCCAAGC TTCCCCTCCG GCGGCAACGA   181 ATGGATCTTG GCGCGCGGCA CTGGGAGCCG CCGGGCCGAG CCTGTCAAGC CCCGCAGCAG   241 CAGCGGCGTC AGGACGGACA TGGTGGTACC GAGCTCGAAT TCTCCAGGCG ATCTGACGGT   301 TCACTAAACG AGCTCTGCTT ATATAGGCCT CCCACCGTAC ACGCCTACCT CGACATACGT   361 TCTCTATCAC TGATAGGGAG TAAACTCGAC ATACGTTCTC TATCACTGAT AGGGATAAAC   421 TCGACATACG TTCTCTATCA CTGATAGGGA GTAAACTCGA CATACGTTCT CTATCACTGA   481 TAGGGAGTAA ACTCGACATA CGTTCTCTAT CACTGATAGG GAGTAAACTC GACATCGTTC   541 TCTATCACTG ATAGGGAGTA AACTCGACAT ACGTTCTCTA TCACTGATAG GGAGTAAACT   601 CGAGATATCT AGACCCAGCT TTCTTGTACA AAGTGGTACG CGTGCGGCCG CAGGAACCCC 661 TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTN //
Primer: BGHpA_F (Pr0373) Region: bGH polyA, attB1, AAV2 ITR Sequence file: RDB18684_B1B5b.seq
>D07612A1_B1B5_1_BGHpA_F_E05_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GGGTGGGGCA GGNNAGCAAG GGGGANGNNT    61 GGGAAGANAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGGCGGC CGCATCGATA   121 AGCTTGTCGA CGATAAAGCC TGCTTTTTTG TACAAACTTG TCACGTGCGG ACCGAGCGGC 181 CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTN //
Primer: CSII_CMV5_v2 (Pr0255) Region: mito-SRAI mid 5' (2xCOX8 pre seq, EYFP) Sequence file: RDB18684_B1B5c.seq
>D07612A1_B1B5_1_CSII-CMV5_V2_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNTNNCCG TCCTGACGCC GCTGCTGCTG    61 CGGGGCTTGA CAGGCTCGGC CCGGCGGCTC CCAGTGCCGC GCGCCAAGAT CCATTCGTTG   121 CCGCCGGAGG GGAAGCTTGG GATGTCCGTC CTGACGCCGC TGCTGCTGCG GGGCTTGACA   181 GGCTCGGCCC GGCGGCTCCC AGTGCCGCGC GCCAAGATCC ATTCGTTGCC GCCGGAGGGG   241 AAGCTCGGGG GATCCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC   301 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG   361 GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGCTGCTCT GCACCACCGG CAAGCTGCCC   421 GTGCCCTGGC CCACCCTCGT GACCACCCTG GGCTACGGCC TGCAGTGCTT CGCCCGCTAC   481 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG   541 GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC   601 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC   661 AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCACCGCC   721 GANNAAGCAG AAGAACGGCA TCAAGGCCNA CTTCNAGATC CGCCACNACA TCGAGGANGG   781 CGNNNNGNAG CTCGCCGACC ACTACCAGCA NANCACCCNC NTCGNNGANG GCCCCGTGCT   841 GCTGCCCGAC AACCACTACC TGANCTACNA GTCCGNCCTG TTCNAAGANC CCCNACNANN   901 AAGCGCGATC ACNNNGNNCC TGCTGGANTN NCTGACCGCN NNCNGGGATC ACNNGAANGC   961 ATGAACNANC TGTNNAANGG ANCTNNGNNG GNANGCNGNT NNNNNNNGAN NTGGNNTCNN  1021 NGNNGNNGGN NNNNNNGNNT CNANNANNGN NNNANCNNNN NNNAANNNNN NNNANNNNAN 1081 NNNTNANNCN NNNNNNNNN //
Primer: mTurquoise2-M_F (Pr0577) Region: mito-SRAI mid (EYFP, hmAzami-Green) Sequence file: RDB18684_B1B5d.seq
>D07612A1_B1B5_1_mTurquoise2-M_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNTACCNG NNGAACACCC CCATCGGCGA    61 CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCGCCC TGTTCAAAGA   121 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC CTGACCGCCG CCGGGATCAC   181 TGAAGGCATG AACGAGCTGT ACAAGGAGCT CGGTGGAGGC GGTTCAGGCG GAGGTGGCTC   241 TGGCGGTGGC GGATCGCTCG AGATGGTGAG CGTGATCAAG CCCGAGATGA AGATCAAGCT   301 GTGCATGAGG GGCACCGTGA ACGGCCACAA CTTCGTGATT GAGGGCGAGG GCAAGGGCAA   361 CCCCTACGAG GGCACCCAGA TCCTGGACCT GAACGTGACC GAGGGCGCCC CCCTGCCCTT   421 CGCCTACGAC ATCCTGTCCA CCTCGTTCCA GTACGGCAAC AGGGCCTTCA CCAAGTACCC   481 CGCCGACATC CAGGACTACT TCAAGCAGGC CTTCCCCGAG GGCTACCACT GGGAGAGGAG   541 CATGACCTAC GAGGACCAGG GCATCTGCAC CGCCACCAGC AACATCAGCA TGAGGGGCGA   601 CTGCTTCTTC TACGACATCA GGTTCGACGG CACCAACTTC CCCCCCAACG GCCCCGTGAT   661 GCAGAAGAAG ACCCTGAAGT GGGAGCCCGA CACCGAGAAG ATGTACGTGG AGGANGGCGT   721 GCTGAAGGGC GACGTGAACA TGAGGCTGCT GCTGGAGGGC GGCGGCCACT ACAGGTGCGA   781 CGTCNAGACC ACCTACAAGG CCAAGAANGA NGTGAGCCTG CCCGACGCCC ACAAGATCGA   841 CCACAGGATC GAGATCCTGG ANCACGANNG GACTACAACA AGGTGANNNT GTGCGAGAAC   901 GNCGNGNNCN GGGCCTCNNT GCTGCCCANC NNNGNCNANG CTANAGCCNG NNNANCNNGN   961 TTCANCAGCC TGANNNATTT NNTGATNCNN NNNGNNAGNT CACNGNNNNN NGNGNNNACN 1021 NNNGNAANCN NANNNNNNNN NN //
Primer: EGFP-C-rev (Pr0026) Region: mito-SRAI mid (EYFP, COX8 pre seq) Sequence file: RDB18684_B1B5e.seq
>D07612A1_B1B5_1_EGFP-C-rev_B04_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NTTNNNNNNN NNNGNNTGGT AGCTCAGGNN NGTGGTTGTC    61 GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG   121 CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTG GCCTTGATGC CGTTCTTCTG   181 CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG TACTCCAGCT TGTGCCCCAG   241 GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC   301 GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT   361 GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG   421 GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG GTGGTCACGA GGGTGGGCCA   481 GGGCACGGGC AGCTTGCCGG TGGTGCAGAG CAGCTTCAGG GTCAGCTTGC CGTAGGTGGC   541 ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC   601 GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGATCCCCC   661 GAGCTTCCCC TCCGGCGGCA ACGAATGGAT CTTGGCGCGC GGCACTGGGA GCCGCCGGGC   721 CGAGCCTGTC AAGCCCCGCA GCAGCAGCGG CGTCAGGACG GACATCCCAN GCTTCCCCTC   781 CGGCGGCAAC GAATGGATCT TGGCGCGCGG CACTGGGAGC CGCCGGGCCG ANCCTGTCAN   841 GCCCCGCANC ANCAGCGGCG TCAGGACGGA CATGGNGNTA CCGANCTCGN ANTTCTCCNN   901 GCGATCTGAC GGNTCACTAA ACGAGCTCTG CTTATATAGG CCTNCCNNCG TACACGCCTA   961 CNNNNNCATA CGNTNNTCTA TNACTGATNN GGNNNNNNAA CNNGNNNNNN CNNTNNTCNA 1021 NNNNNGNNNN NGNNNNNNAA ANNTNNNNNN NNNN //
Primer: bGH_rev3 (Pr0885) Region: mito-SRAI 3' (hmAzami-Green) Sequence file: RDB18684_B1B5f.seq
>D07612A1_B1B5_1_bGH_rev3_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NGGCNNNNNN NNAGNTGGCT GGCNAACTAG    61 AAGGCACNNN NNNGTCAGCT GACTNNAGGA NCCTACACAT TGATCCTAGC AGAAGCACAG   121 GCTGCAGGGT GACGGTCCAT CCCGCTCTCC TGGGCACAAG ACATGGGCAG CGTGCCATCA   181 TCCTGCTCCT CCACCTCCGG CGGGAAGCCA TGGCTCTGGA TCTGTTTCAT GAGTTGCCAC   241 ATTGGCCGTG ATCTAGCCAG GTGGATCACG AAATGGCTCA GGCTGCTGAA CCAGTTCTTG   301 CAGGCTCTAG CCTTGGCCTG GCTGGGCAGC ATGGAGGCCC TGGCCACGGC GTTCTCGCAC   361 AGCTTCACCT TGTTGTAGTC CTTGTCGTGC TCCAGGATCT CGATCCTGTG GTCGATCTTG   421 TGGGCGTCGG GCAGGCTCAC CTCCTTCTTG GCCTTGTAGG TGGTCTTGAC GTCGCACCTG   481 TAGTGGCCGC CGCCCTCCAG CAGCAGCCTC ATGTTCACGT CGCCCTTCAG CACGCCGTCC   541 TCCACGTACA TCTTCTCGGT GTCGGGCTCC CACTTCAGGG TCTTCTTCTG CATCACGGGG   601 CCGTTGGGGG GGAAGTTGGT GCCGTCGAAC CTGATGTCGT AGAAGAAGCA GTCGCCCCTC   661 ATGCTGATGT TGCTGGTGGC GGTGCAGATG CCCTGGTCCT CGTANGNNNN GNTCCTCTCC   721 CNGTGGTAGC CCTCGGGGAA NGCCTGCTTG AAGTAGTCCT GNATGTCGGC GGGGTACTTG   781 GTGAAGGCCC TGTTGCCGTA CTGNANCGAG GTGNNNAGGA TGTCGTANGC GAANNGNNAG   841 GGGGGCGCCC TCNGTNNCGT NCANGNNNNN GATCTGGGNG CCCTCGTANG GNNNGCCCNT   901 GCCCTCNCCC TCAANCNCNN NNNNGTGGCC GTTCACNGNN GCCCCTNNNG NNNNNCTTGA   961 TNNTNNNTCN CNGGNNNNNT CNNGCTCACN NTCNNNNNNN CNANNCNNNC NNNNNCCNNN 1021 ANCNNNNCNN CCNNNNNGNA ANNNNCNNNN NNCNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl