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YC3.60/pRSETB

Yellow cameleon calcium ion sensor clone YC3.60.

Catalog number RDB15136
Resource name YC3.60/pRSETB
Clone info. Calcium ion sensor clone YC3.60 Yellow cameleon having E104Q substitution in the 3rd Ca2+ binding site of CaM and the circular permutated cp173Venus. Bacterial expression plasmid.
Known differences (at least): C to T at 291nt (97aa, Arg, synonym), T to C at 438nt (146aa, Tyr, synonym), A to C at 493nt (165aa, Asn to His), G to C at 639nt (213aa, Lys to Asn) of AB178712.1. Nucleotide sequence of AB178712.1 will be updated.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AB178712 (DDBJ accession number)
Depositor Miyawaki, Atsushi |
 
Other clones in our bank X. laevis (KEGG orthology K02183) | synthetic (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Remarks
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15136 YC3.60/pRSETB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Calcium Ion Sensor Clones (English text)

References

original Miyawaki, A., Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). PMID 24184769.
original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15136_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15136_A7H9a.seq
>D04498C3_A7H9_1_T7_20bp_H10_22_ABI24.ab1
    1 GGAAAACTCG GCTAGGAATA ATTTTGTTTA CTTTAAGAAG GAGGATATAC ATATGCGGGG
   61 TTCTCATCAT CATCATCATC ATGGTATGGC TAGCATGACT GGTGGACAGC AAATGGGTCG
  121 GGATCTGTAC GACGATGACG ATAAGGATCC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC
  181 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA GGTTCAGCGT
  241 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC
  301 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT GGGGCGTGCA
  361 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC
  421 CGAAGGCTAC GTCCAGGAGC GTACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG
  481 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA
  541 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA TCAGCCACAA
  601 CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCCACTTCA AGATCCGCCA
  661 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG
  721 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA
  781 AGACCCCAAC GAGAAGCGCG ATCACATGGG TCCTGCTGGA GTTCGTGACC GCCCGCCCGC
  841 ATGCATGACC AACTGACAGA AGAGCAGATT GCAGAGTTTC AAGAAGCCTT CCTCATTATC
  901 GACAGATGGG GACGGCACCA TCCACCCACA AGGAACTGGC ACCGTTATGA AGTCCGCTGA
  961 CAAATCCCAC GAGCAGAATG CAGGAAATTG GATCAATGAA GTCGAATGCT GATGGCATTG
1021 GACGATTACC TTCCTGATCT ACCATTGATG CTGAATGAGA CCTGAACGCG GAGGAATC
//
Primer: f1ori_R3 RDB15136_A7H9b.seq
>D04498C3_A7H9_1_f1ori_R3_E05_14_ABI24.ab1
    1 GGATCTTACT TTTTTTTTCC ATCAGCAGCG CACTGTTGGG AGGGCGATCG GTGCGGGCCT
   61 CTTCGCTATT ACGCCAGATC CGGATATAGT TCCTCCTTTC AGCAAAAAAC CCCTCAAGAC
  121 CCGTTTAGAG GCCCCAAGGG GTTATGCTAG TTATTGCTCA GCGGTGGCAG CAGCCAACTC
  181 AGCTTCCTTT CGGGCTTTGT TAGCAGCCGG ATCAAGCTTC GAATTCTTAC TCGATGTTGT
  241 GGCGGATCTT GAAGTTGGCC TTGATGCCGT TCTTCTGCTT GTCGGCGGTG ATATAGACGT
  301 TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT
  361 CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC
  421 GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG
  481 GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG GCGAAGCACT
  541 GCAGGCCGTA GCCCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG
  601 TGCAGATCAG CTTCAGGGTC AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA
  661 CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG
  721 TGAACAGCTC CTCGCCCTTG CTCACCATGC CACCGCTGCC ACCCTTGTAC AGCTCGTCCA
  781 TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT
  841 CGTTGGGGTC TTTGCTCAGG GCGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA
  901 CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT
  961 CCATGAGCTC CAGTGCCCCG GGAGCTGGAG ATCTTCTTGA ACCGGTTGGC AGCGCTGACG
 1021 GGCAATGAAG TTTTTTCTTC CAGCGCCTCT TCCCCCCCCC TTTGCTGTCA TCATTTGGTA
 1081 CAACTCTTCA TAGTTAACTT GGGCCATCAC CATCGATATT CTGCTGCCTA TCAATTTCCA
1141 TTCCACCTTC TTTCAATCTG GTTAACCTTT CTCTTCCCCC GCAGAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Miyawaki, A., Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). PMID 24184769.
original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

2019.03.18

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