RDB15136 - YC3.60/pRSETB

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

YC3.60/pRSETB (#RDB15136)

Yellow cameleon calcium ion sensor clone YC3.60.

Clone info.	Calcium ion sensor clone YC3.60 Yellow cameleon having E104Q substitution in the 3rd Ca2+ binding site of CaM and the circular permutated cp173Venus. Bacterial expression plasmid.
Comment	Known differences (at least): C to T at 291nt (97aa, Arg, synonym), T to C at 438nt (146aa, Tyr, synonym), A to C at 493nt (165aa, Asn to His), G to C at 639nt (213aa, Lys to Asn) of AB178712.1. Nucleotide sequence of AB178712.1 will be updated.
Vector backbone	pRSETB (plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Amp^r
Growth conditions	LB + Amp, 37oC
Gene/insert name	X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of insert sequence	AB178712 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

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Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Proc. Natl. Acad. Sci. U S A. 101:10554-10559 (2004).

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Proc. Natl. Acad. Sci. U S A. 101:10554-10559 (2004).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Proc. Natl. Acad. Sci. U S A. 101:10554-10559 (2004).
備考	提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15136	YC3.60/pRSETB	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The YC3.60/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15136).

Reference section:

Miyawaki, A., Nagai, T., Mizuno, H., Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). PMID 24184769. [link to RRC of NBRP]

Nagai, T., Yamada, S., Tominaga, T., Ichikawa, M., Miyawaki, A., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15136_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB15136_A7H9a.seq
>D04498C3_A7H9_1_T7_20bp_H10_22_ABI24.ab1 1 GGAAAACTCG GCTAGGAATA ATTTTGTTTA CTTTAAGAAG GAGGATATAC ATATGCGGGG 61 TTCTCATCAT CATCATCATC ATGGTATGGC TAGCATGACT GGTGGACAGC AAATGGGTCG 121 GGATCTGTAC GACGATGACG ATAAGGATCC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC 181 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA GGTTCAGCGT 241 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC 301 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT GGGGCGTGCA 361 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC 421 CGAAGGCTAC GTCCAGGAGC GTACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG 481 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA 541 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA TCAGCCACAA 601 CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCCACTTCA AGATCCGCCA 661 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG 721 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA 781 AGACCCCAAC GAGAAGCGCG ATCACATGGG TCCTGCTGGA GTTCGTGACC GCCCGCCCGC 841 ATGCATGACC AACTGACAGA AGAGCAGATT GCAGAGTTTC AAGAAGCCTT CCTCATTATC 901 GACAGATGGG GACGGCACCA TCCACCCACA AGGAACTGGC ACCGTTATGA AGTCCGCTGA 961 CAAATCCCAC GAGCAGAATG CAGGAAATTG GATCAATGAA GTCGAATGCT GATGGCATTG 1021 GACGATTACC TTCCTGATCT ACCATTGATG CTGAATGAGA CCTGAACGCG GAGGAATC //
Primer: f1ori_R3 Sequence file: RDB15136_A7H9b.seq
>D04498C3_A7H9_1_f1ori_R3_E05_14_ABI24.ab1 1 GGATCTTACT TTTTTTTTCC ATCAGCAGCG CACTGTTGGG AGGGCGATCG GTGCGGGCCT 61 CTTCGCTATT ACGCCAGATC CGGATATAGT TCCTCCTTTC AGCAAAAAAC CCCTCAAGAC 121 CCGTTTAGAG GCCCCAAGGG GTTATGCTAG TTATTGCTCA GCGGTGGCAG CAGCCAACTC 181 AGCTTCCTTT CGGGCTTTGT TAGCAGCCGG ATCAAGCTTC GAATTCTTAC TCGATGTTGT 241 GGCGGATCTT GAAGTTGGCC TTGATGCCGT TCTTCTGCTT GTCGGCGGTG ATATAGACGT 301 TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT 361 CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC 421 GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG 481 GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG GCGAAGCACT 541 GCAGGCCGTA GCCCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG 601 TGCAGATCAG CTTCAGGGTC AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA 661 CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG 721 TGAACAGCTC CTCGCCCTTG CTCACCATGC CACCGCTGCC ACCCTTGTAC AGCTCGTCCA 781 TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT 841 CGTTGGGGTC TTTGCTCAGG GCGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA 901 CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT 961 CCATGAGCTC CAGTGCCCCG GGAGCTGGAG ATCTTCTTGA ACCGGTTGGC AGCGCTGACG 1021 GGCAATGAAG TTTTTTCTTC CAGCGCCTCT TCCCCCCCCC TTTGCTGTCA TCATTTGGTA 1081 CAACTCTTCA TAGTTAACTT GGGCCATCAC CATCGATATT CTGCTGCCTA TCAATTTCCA 1141 TTCCACCTTC TTTCAATCTG GTTAACCTTT CTCTTCCCCC GCAGAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Miyawaki, A., Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). PMID 24184769. [link to RRC of NBRP]
original	Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. [link to RRC of NBRP]