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YC3.60pm/pRSETB

Yellow cameleon calcium ion sensor clone YC3.60pm wiht plasma membrane localization tag.

Catalog number RDB15137
Resource name YC3.60pm/pRSETB
Clone info. Calcium ion sensor clone YC3.60 Yellow cameleon having plasma membrane localization tag, E104Q substitution in the 3rd Ca2+ binding site of CaM and the circular permutated cp173Venus. Bacterial expression plasmid.
The CAAX box of Ki-Ras was fused to the C terminus of YC3.60 through a linker sequence
(GTGGSGGGTGGSGGGT).
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r (E. coli)
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AB178714 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank X. laevis (KEGG orthology K02183) | synthetic (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15137 YC3.60pm/pRSETB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence compiled by us RDB15137zzt01.seq

Original reference

original Miyawaki, A., Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). PMID 24184769.
original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Calcium Ion Sensor Clones (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15137_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15137_A7H9a.seq check
>15137_15137_B0KrF_lacIpro-B_F_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCGCA NCGNNNCNGT GNGCGANGAA GCGGAAGAGC GCCCAATACG
   61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGGATCTCGA TCCCGCGAAA
  121 TTAATACGAC TCACTATAGG GAGACCACAA CGGTTTCCCT CTAGAAATAA TTTTGTTTAA
  181 CTTTAAGAAG GAGATATACA TATGCGGGGT TCTCATCATC ATCATCATCA TGGTATGGCT
  241 AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGATCCC
  301 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
  361 GGCGACGTAA ACGGCCACAG GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
  421 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
  481 CTCGTGACCA CCCTGACCTG GGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
  541 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG TACCATCTTC
  601 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
  661 GTGAACCGCA TCGAGCTGAA NGGCATCGAC TTCAAGGANG ACGGCAACAT CCTGGGGCAC
  721 AAGCTGGAGT ACAACTACAT CAGCCACAAC GTCTATATCA CCGCCGANAA GCAGAAGAAC
  781 GGCATCAAGG CCCACTTCAA GATCCGCCAC AACATCGAGG ANGGCAGCGN GCAGCTCGCC
  841 NACCACTACC AGCAGAACAC CCNCATCGGC GANGGCCCCG NNCTGCTGCC CGACAACCAC
  901 TACCNGANCA CCCNNNNCGC CCNNNNCAAA GANCNNNANG ANAANCGCGN NNNCNNNGNC
961 CNGNNNGNNN NNNNNNN
//
Primer: T7ter_R1
Region: insert 3'
Sequence file: RDB15137_A7H9b.seq check
>15137_15137_B0KrF_T7ter_R1_B07_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NNTNNNTGCT NNGCGGTGGC AGCAGCCNAC TCAGCTTCCT
   61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACATAATTAC ACACTTTGTC
  121 TTTGACTTCT TTTTCTTCTT TTTACCATCT TTGCTCATCT TGGTACCGGA GCCGCCGCTG
  181 CCGCCGGTAC CGGAGCCGCC GCTGCCGCCG GTACCCTCGA TGTTGTGGCG GATCTTGAAG
  241 TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG
  301 TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC
  361 TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG
  421 CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG
  481 AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGGCGA AGCACTGCAG GCCGTAGCCC
  541 AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATCAGCTTC
  601 AGGGTCAGCT TGCCGTANGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG
  661 CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG
  721 CCCTTGCTCA CCATGCCACC GCTGCCACCC TTGTACAGCT CGTCCATGCC GANAGTGATC
  781 CCGGCGGCGG TCACGAACTC CAGCANGNNC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG
  841 CTCANGGCGG NNNGGTANCT CANGTAGTGN TTGTCNGGCA GCANCACGGG GCCNNCGCCN
  901 ATGGGGNNGT TCTGCNNGNA NTGGTCNGNN ANCNGCACGN CNNNNNNCAT NANCNNCNNN
961 NNCCCNNNNN NNGNNNNNNN NNN
//
Primer: FP-N_F1
Region: insert mid
Sequence file: RDB15137_A7H9c.seq check
>15137_15137_B0KrF_FP-N_F1_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCN TGNAGCAGCA CGACTTCTTC NAGTCCGCCA
   61 TGCCCGAAGG CTACGTCCAG GAGCGTACCA TCTTCTTCAA GGACGACGGC AACTACAAGA
  121 CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA
  181 TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACATCAGCC
  241 ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCCAC TTCAAGATCC
  301 GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA
  361 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA
  421 GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCC
  481 GCATGCATGA CCAACTGACA GAAGAGCAGA TTGCAGAGTT CAAAGAAGCC TTCTCATTAT
  541 TCGACAAGGA TGGGGACGGC ACCATCACCA CAAAGGAACT TGGCACCGTT ATGAGGTCGC
  601 TTGGACAAAA CCCAACGGAA GCAGAATTGC AGGATATGAT CAATGAAGTC GATGCTGATG
  661 GCAATGGAAC GATTTACTTT CCTGAATTTC TTACTATGAT GGCTAGAAAA ATGAAGGACA
  721 CAGACAGCGA AGAGGAAATC CGAGAAGCAT TCCGTGTTTT TGACAAGGAT GGGAACGGCT
  781 ACATCAGCGC TGCTCAGTTA CGTCACGTCA TGACAAACCT CGGGGANAAG TTAACAGATG
  841 AAGAAGTTGA TGAAATGATA AGGNAAGCAG ATATCGATGG TGATGGNCNA GTAAACTATG
  901 AAGAGTTTGT ACAAATGATG ACAGCAAANG GGGGGAAGAN GNGCNNGNNN AAAAAACTNC
961 NNNNNNNNCA GCGCNNNNNN CCGGNNCNNN NANNNNNNNC NNNNNNNGGN NNNNNNNNN
//
Primer: YC4.6-M_F
Region: insert mid
Sequence file: RDB15137_A7H9d.seq check
>15137_15137_B0KrF_YC4.6-M_F_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC NTNNNGANGT CGCTTGGNNN AAACCCAACG GAAGCAGAAT
   61 TGCAGGATAT GATCAATGAA GTCGATGCTG ATGGCAATGG AACGATTTAC TTTCCTGAAT
  121 TTCTTACTAT GATGGCTAGA AAAATGAAGG ACACAGACAG CGAAGAGGAA ATCCGAGAAG
  181 CATTCCGTGT TTTTGACAAG GATGGGAACG GCTACATCAG CGCTGCTCAG TTACGTCACG
  241 TCATGACAAA CCTCGGGGAG AAGTTAACAG ATGAAGAAGT TGATGAAATG ATAAGGGAAG
  301 CAGATATCGA TGGTGATGGC CAAGTAAACT ATGAAGAGTT TGTACAAATG ATGACAGCAA
  361 AGGGGGGGAA GAGGCGCTGG AAGAAAAACT TCATTGCCGT CAGCGCTGCC AACCGGTTCA
  421 AGAAGATCTC CAGCTCCGGG GCACTGGAGC TCATGGACGG CGGCGTGCAG CTCGCCGACC
  481 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
  541 TGAGCTACCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
  601 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGTGGCA
  661 GCGGTGGCAT GGTGAGCAAG GGCGAGGANC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG
  721 ANCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGANGGC GANGGCGATG
  781 CCACCTACNG CAAGCTGACC CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT
  841 NNCCCACCCT CNNGANCACC CTGGGCTACN GNCNGCANNG CTTCGCCCGC TANCCCGNNC
  901 ACATGANNCA NCNCGANTTC NNCNAGNCCG NCNTGNCCGN ANNNNACNNN CAGGNNCGCA
961 NNATCNTNNN NNNANNNNNN NNNGNNNNNN NNNN
//
Primer: EYFP-C_F
Region: insert mid
Sequence file: RDB15137_A7H9e.seq check
>15137_15137_B0KrF_EYFP-C_F_E07_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCGCGNNC NCNTGGTCCT GCTGGNNTTC GTGNCCGCCG
   61 CCGGGNTCAC TCTCGGCATG GACGAGCTGT ACAAGGGTGG CAGCGGTGGC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG
  601 CCAACTTCAA GATCCGCCAC AACATCGAGG GTACCGGCGG CAGCGGCGGC TCCGGTACCG
  661 GCGGCAGCGG CGGCTCCGGT ACCAAGATGA GCAAAGATGG TAAAAAGAAG AAAAAGAAGT
  721 CAAAGACAAA GTGTGTAATT ATGTAAGAAN TCGAANCTTG ATCCGGCTGC TAACAAAGCC
  781 CGAAAGGAAG CTGANTTGGC TGCTGCCACC GCTNANCAAT AACTAGCATA ACCCCTNGGG
  841 GCCTCTAAAC GGGTCTTGAN GGNTTTTTTT GCTGAAAGGA NGAACTATAT CCGGATCTGG
  901 CGNAATANCG AANANGCCCG CANCNATCGC CCNTCCNNAN AGTNGCNCAN CCNNNNNGGN
961 NAANNGNANG CGNCNNGNAN CGGNNNNNNN NNCNNNGNNN NNNNNGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Miyawaki, A., Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). PMID 24184769.
original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

2021.01.12

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