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YC3.60pm/pRSETB

Yellow cameleon calcium ion sensor clone YC3.60pm wiht plasma membrane localization tag.

Catalog number RDB15137
Resource name YC3.60pm/pRSETB
Clone info. Calcium ion sensor clone YC3.60 Yellow cameleon having plasma membrane localization tag, E104Q substitution in the 3rd Ca2+ binding site of CaM and the circular permutated cp173Venus. Bacterial expression plasmid.
Comment The CAAX box of Ki-Ras was fused to the C terminus of YC3.60 through a linker sequence
(GTGGSGGGTGGSGGGT).
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r (E. coli)
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AB178714 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件は英文のまま記載してください。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15137 YC3.60pm/pRSETB DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Sequence (full) RDB15137zzt01.seq compiled by us

        Original reference

        original Miyawaki, A., Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). PMID 24184769.
        original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15137_A7H9p1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: lacIpro-B_F
        Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
        Sequence file: RDB15137_A7H9a.seq check
        >15137_15137_B0KrF_lacIpro-B_F_A07_01_ABI24.ab1
            1 NNNNNNNNNN NNNNNNCGCA NCGNNNCNGT GNGCGANGAA GCGGAAGAGC GCCCAATACG
           61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGGATCTCGA TCCCGCGAAA
          121 TTAATACGAC TCACTATAGG GAGACCACAA CGGTTTCCCT CTAGAAATAA TTTTGTTTAA
          181 CTTTAAGAAG GAGATATACA TATGCGGGGT TCTCATCATC ATCATCATCA TGGTATGGCT
          241 AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGATCCC
          301 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
          361 GGCGACGTAA ACGGCCACAG GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
          421 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
          481 CTCGTGACCA CCCTGACCTG GGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
          541 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG TACCATCTTC
          601 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
          661 GTGAACCGCA TCGAGCTGAA NGGCATCGAC TTCAAGGANG ACGGCAACAT CCTGGGGCAC
          721 AAGCTGGAGT ACAACTACAT CAGCCACAAC GTCTATATCA CCGCCGANAA GCAGAAGAAC
          781 GGCATCAAGG CCCACTTCAA GATCCGCCAC AACATCGAGG ANGGCAGCGN GCAGCTCGCC
          841 NACCACTACC AGCAGAACAC CCNCATCGGC GANGGCCCCG NNCTGCTGCC CGACAACCAC
          901 TACCNGANCA CCCNNNNCGC CCNNNNCAAA GANCNNNANG ANAANCGCGN NNNCNNNGNC
        961 CNGNNNGNNN NNNNNNN
        //
        Primer: T7ter_R1
        Region: insert 3'
        Sequence file: RDB15137_A7H9b.seq check
        >15137_15137_B0KrF_T7ter_R1_B07_04_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNGNN NNTNNNTGCT NNGCGGTGGC AGCAGCCNAC TCAGCTTCCT
           61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACATAATTAC ACACTTTGTC
          121 TTTGACTTCT TTTTCTTCTT TTTACCATCT TTGCTCATCT TGGTACCGGA GCCGCCGCTG
          181 CCGCCGGTAC CGGAGCCGCC GCTGCCGCCG GTACCCTCGA TGTTGTGGCG GATCTTGAAG
          241 TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG
          301 TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC
          361 TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG
          421 CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG
          481 AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGGCGA AGCACTGCAG GCCGTAGCCC
          541 AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATCAGCTTC
          601 AGGGTCAGCT TGCCGTANGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG
          661 CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG
          721 CCCTTGCTCA CCATGCCACC GCTGCCACCC TTGTACAGCT CGTCCATGCC GANAGTGATC
          781 CCGGCGGCGG TCACGAACTC CAGCANGNNC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG
          841 CTCANGGCGG NNNGGTANCT CANGTAGTGN TTGTCNGGCA GCANCACGGG GCCNNCGCCN
          901 ATGGGGNNGT TCTGCNNGNA NTGGTCNGNN ANCNGCACGN CNNNNNNCAT NANCNNCNNN
        961 NNCCCNNNNN NNGNNNNNNN NNN
        //
        Primer: FP-N_F1
        Region: insert mid
        Sequence file: RDB15137_A7H9c.seq check
        >15137_15137_B0KrF_FP-N_F1_C07_07_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCN TGNAGCAGCA CGACTTCTTC NAGTCCGCCA
           61 TGCCCGAAGG CTACGTCCAG GAGCGTACCA TCTTCTTCAA GGACGACGGC AACTACAAGA
          121 CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA
          181 TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACATCAGCC
          241 ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCCAC TTCAAGATCC
          301 GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA
          361 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA
          421 GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCC
          481 GCATGCATGA CCAACTGACA GAAGAGCAGA TTGCAGAGTT CAAAGAAGCC TTCTCATTAT
          541 TCGACAAGGA TGGGGACGGC ACCATCACCA CAAAGGAACT TGGCACCGTT ATGAGGTCGC
          601 TTGGACAAAA CCCAACGGAA GCAGAATTGC AGGATATGAT CAATGAAGTC GATGCTGATG
          661 GCAATGGAAC GATTTACTTT CCTGAATTTC TTACTATGAT GGCTAGAAAA ATGAAGGACA
          721 CAGACAGCGA AGAGGAAATC CGAGAAGCAT TCCGTGTTTT TGACAAGGAT GGGAACGGCT
          781 ACATCAGCGC TGCTCAGTTA CGTCACGTCA TGACAAACCT CGGGGANAAG TTAACAGATG
          841 AAGAAGTTGA TGAAATGATA AGGNAAGCAG ATATCGATGG TGATGGNCNA GTAAACTATG
          901 AAGAGTTTGT ACAAATGATG ACAGCAAANG GGGGGAAGAN GNGCNNGNNN AAAAAACTNC
        961 NNNNNNNNCA GCGCNNNNNN CCGGNNCNNN NANNNNNNNC NNNNNNNGGN NNNNNNNNN
        //
        Primer: YC4.6-M_F
        Region: insert mid
        Sequence file: RDB15137_A7H9d.seq check
        >15137_15137_B0KrF_YC4.6-M_F_D07_10_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNC NTNNNGANGT CGCTTGGNNN AAACCCAACG GAAGCAGAAT
           61 TGCAGGATAT GATCAATGAA GTCGATGCTG ATGGCAATGG AACGATTTAC TTTCCTGAAT
          121 TTCTTACTAT GATGGCTAGA AAAATGAAGG ACACAGACAG CGAAGAGGAA ATCCGAGAAG
          181 CATTCCGTGT TTTTGACAAG GATGGGAACG GCTACATCAG CGCTGCTCAG TTACGTCACG
          241 TCATGACAAA CCTCGGGGAG AAGTTAACAG ATGAAGAAGT TGATGAAATG ATAAGGGAAG
          301 CAGATATCGA TGGTGATGGC CAAGTAAACT ATGAAGAGTT TGTACAAATG ATGACAGCAA
          361 AGGGGGGGAA GAGGCGCTGG AAGAAAAACT TCATTGCCGT CAGCGCTGCC AACCGGTTCA
          421 AGAAGATCTC CAGCTCCGGG GCACTGGAGC TCATGGACGG CGGCGTGCAG CTCGCCGACC
          481 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
          541 TGAGCTACCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
          601 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGTGGCA
          661 GCGGTGGCAT GGTGAGCAAG GGCGAGGANC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG
          721 ANCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGANGGC GANGGCGATG
          781 CCACCTACNG CAAGCTGACC CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT
          841 NNCCCACCCT CNNGANCACC CTGGGCTACN GNCNGCANNG CTTCGCCCGC TANCCCGNNC
          901 ACATGANNCA NCNCGANTTC NNCNAGNCCG NCNTGNCCGN ANNNNACNNN CAGGNNCGCA
        961 NNATCNTNNN NNNANNNNNN NNNGNNNNNN NNNN
        //
        Primer: EYFP-C_F
        Region: insert mid
        Sequence file: RDB15137_A7H9e.seq check
        >15137_15137_B0KrF_EYFP-C_F_E07_13_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNCGCGNNC NCNTGGTCCT GCTGGNNTTC GTGNCCGCCG
           61 CCGGGNTCAC TCTCGGCATG GACGAGCTGT ACAAGGGTGG CAGCGGTGGC ATGGTGAGCA
          121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
          181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
          241 CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
          301 CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT
          361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
          421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
          481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
          541 ACAACTACAA CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG
          601 CCAACTTCAA GATCCGCCAC AACATCGAGG GTACCGGCGG CAGCGGCGGC TCCGGTACCG
          661 GCGGCAGCGG CGGCTCCGGT ACCAAGATGA GCAAAGATGG TAAAAAGAAG AAAAAGAAGT
          721 CAAAGACAAA GTGTGTAATT ATGTAAGAAN TCGAANCTTG ATCCGGCTGC TAACAAAGCC
          781 CGAAAGGAAG CTGANTTGGC TGCTGCCACC GCTNANCAAT AACTAGCATA ACCCCTNGGG
          841 GCCTCTAAAC GGGTCTTGAN GGNTTTTTTT GCTGAAAGGA NGAACTATAT CCGGATCTGG
          901 CGNAATANCG AANANGCCCG CANCNATCGC CCNTCCNNAN AGTNGCNCAN CCNNNNNGGN
        961 NAANNGNANG CGNCNNGNAN CGGNNNNNNN NNCNNNGNNN NNNNNGNNNN NNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Miyawaki, A., Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). PMID 24184769.
        original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl