Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAAV2 TRE Venus-Akaluc

Expression vector of Akaluc luciferase.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-TRE-GFP-BGHpA vector (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. 付加的提供条件： 利用者は研究成果の公表にあたって、pENTR1A-TRE-GFP-BGHpA の文献 (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009) を引用し、順天堂大学日置寛之先生への謝辞の表明を必要とする。 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15783_A8B8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Venus-N_R (Pr0341) Region: insert 5'(Venus 5',tight TRE pro),attB2,AAV2 ITR Sequence file: RDB15783_A8B8e.seq
>D04498K8_A8B8_1_Venus-N_R_D02_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGGNCG TTNNNGTCGC CGTCCNGCTC GANCAGGNNG    61 GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGTGGCGGT ACCGAGCTCG   121 AATTCTCCAG GCGATCTGAC GGTTCACTAA ACGAGCTCTG CTTATATAGG CCTCCCACCG   181 TACACGCCTA CCTCGACATA CGTTCTCTAT CACTGATAGG GAGTAAACTC GACATACGTT   241 CTCTATCACT GATAGGGATA AACTCGACAT ACGTTCTCTA TCACTGATAG GGAGTAAACT   301 CGACATACGT TCTCTATCAC TGATAGGGAG TAAACTCGAC ATACGTTCTC TATCACTGAT   361 AGGGAGTAAA CTCGACATCG TTCTCTATCA CTGATAGGGA GTAAACTCGA CATACGTTCT   421 CTATCACTGA TAGGGAGTAA ACTCGAGATA TCTAGACCCA GCTTTCTTGT ACAAAGTGGT 481 ACGCGTGCGG CCGCAGGAAC CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTN //
Primer: Akaluc 1215-1243 (Pr0540) Region: insert 3'(Akaluc 3',bGH pA),attB1 AAV2 ITR Sequence file: RDB15783_A8B8f.seq
>D04498K8_A8B8_1_Akaluc 1215-1243_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNN NNNGNNNNNC NNTTCTTCNT CGTGGNNCGG    61 CTGAAGAGCC TGATCAAATA CAAGGGCTAC CAGGTAGCCC CAGCCGAACT GGAGGGCATC   121 CTGCTGCAAC ACCCCTACAT CTTCGACGCC GGAGTCGCCG GCCTGCCCGA CGACGATGCC   181 GGCGAGCTGC CCGCCGCAGT CGTCGTGTTG GAACACGGTA AAACCATGAC CGAGAAAGAG   241 ATCGTGGACT ATGTGGCCAG CCAGGTTACA ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG   301 TTTGTGGATG AAGTCCCTAG AGGATCGACC GGCAAGTTAG ACGCCCGCAA GATCCGCGAG   361 ATTCTCACTA AGGCCAAGAA GGACGGCAAG ATCGCCGTGT AAACGCGTCT GTGCCTTCTA   421 GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA   481 CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC   541 ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA   601 GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGGCGGCCG CATCGATAAG CTTGTCGACG   661 ATAAAGCCTG CTTTTTTGTA CAAACTTGTC ACGTGCGGAC CGAGCGGCCG CAGGAACCCC 721 TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGNN //
Primer: D03160F1 (Pr0357) Region: insert mid(Venus 5') Sequence file: RDB15783_A8B8g.seq
>15783_15783_B2EeF_1_D03160F1_C02_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GAGAANTCGA GCTCGGTACC GCCACCATGG    61 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG   121 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA   181 AGCTGACCCT GAAGCTGATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG   241 TGACCACCCT GGGCTACGGC CTGCAGTGCT TCGCCCGCTA CCCCGACCAC ATGAAGCAGC   301 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA   361 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA   421 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC   481 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCACCGC CGACAAGCAG AAGAACGGCA   541 TCAAGGCCAA CTTCAAGATC CGCCACAACA TCNAGGACGG CGGCGTGCAG CTCGCCGACN   601 ACTACCANCA GANCACCCCC NTCGGCGANN GNCCCGTGCT GCTGCCCGAC AACCACTANC   661 TGANCTACCA GTCCNNCCNN ANNNAAGANC CCNANGANNA NNNNNNTNNN NNNGNNCNNG 721 CNNNNNNNNN N //
Primer: pGL4ins_R (Pr0666) Region: insert mid(Akaluc 5',Venus 3') Sequence file: RDB15783_A8B8h.seq
>15783_15783_B2EeF_1_pGL4ins_R_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNAANG GCGCTGGGCC CTTCTTAATG TTTTTGGCAT    61 CTTCCATGGA TCCCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA   121 ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGT   181 AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT   241 GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTGG   301 CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG TTGTAGTTGT   361 ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA   421 TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT   481 CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA   541 AGTCGTGCTG CTTCATGTGN NNNGGGTAGC GGGCGAANCA CTGCAGGCCG TANCCCAGGG   601 TGGTCACGAG GGTGGGCCAG GGCNNNGGCA GCTTGCCGGN GGNGCANATC NNCTTCNNGG   661 TCNNCTTGNC NGTNNNGGCN TCNNCCTCGC CCNCNCCGGA NNCNCNNAAN TTGNNGNNNT 721 TNNNNTCNCC NNCNNNTNNA NCNNNNNGGG NNNNNNNNNG GNNNNNNNNN N //
Primer: Venus-C_F2 (Pr0454) Region: insert mid(Akaluc mid) Sequence file: RDB15783_A8B8i.seq
>D04498K8_A8B8_1_Venus-C_F2_A02_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCTC NNNNNGGNNG ANCTGTACNA GGGNNCCNTG    61 GAAGANGCCN AAAACATTAA GAAGGGCCCA GCGCCGTTCT ACCCACTCGA AGACGGGACC   121 GCCGGCGAGC AGCTGCACAA AGCCATGAAG CGCTACGCCC TGGTGCCCGG CGCCATCGCC   181 TTTACCGACG CACATATTCA GGTGGACGTT ACCTACGCCG AGTACTTCGA GATGAGCGTT   241 CGGCTGGCAG AAGCTATGAG GCGCTATGGG CTGAATACAA ACCATCGGAT CGTGGTGTGC   301 AGCGAGAATA GCTCGCAGTT CTTCATGCCC GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT   361 GTGGCCCCAG CTAACGACAT CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC   421 CAGCCCACCG TCGTATTCGT GAGCAAGAAA GGGCTGCGAA AGGTCCTCAA CGTGCAAAAG   481 AAGCTACCGA TCATACGAAA GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC   541 CAAAGCATGT ACACCTTCGT GACTTCCCAT TTGCCACCCA GCTTCAACGA GTACGACTTC   601 GTGCCCGAGA GCTTCGACCG GGACAAAACC ATCGCCCTGA TCATGAACAG TAGTGGTAGT   661 ACAGGATTAC CCAAGGGCGT AGCCCTACCG CACCGCACCG CTTGTGTCCG ATTCAGTCAT   721 GCCCGCGACC CCATCTTCGG CTACCAGAAC ATCCCCGACA CCGCTATCCT CAGCGTGGTG   781 CCATTTCACC ACGGCTTCNG CATGTTCACC ACGCTGGGCT ACTTGATCTG CGGCTTTCGG   841 GNCGNGCTCA TGTANCGCTT NNNNGAGGAN CTATTCTTGC GCAGCTNGCN AGANTATAAG   901 ANNCAATCTG CCNNGCNNNN GCCCACACTA TTTANCTGCN NCGCTAAGAN CNNTNTCATC 961 NACAANNNCN NNCNNNNNNG CTNGCNCGAN ANNNNNNNNN N //
Primer: Akaluc 576-593 (Pr0539) Region: insert mid(Akaluc mid) Sequence file: RDB15783_A8B8j.seq
>D04498K8_A8B8_1_Akaluc 576-593_C02_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCCGCN NCNNNNNNCT TGTGTNNNNN NCNGTCATGC    61 CCGCGACCCC ATCTTCGGCT ACCAGAACAT CCCCGACACC GCTATCCTCA GCGTGGTGCC   121 ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT   181 CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT   241 TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTGCCTC GCTAAGAGCA CTCTCATCGA   301 CAAGTACGAC CTAAGCAGCT TGCGCGAGAT CGCCAGCGGC GGGGCGCCGC TCAGCAAGGA   361 GGTAGGTGAG GCCGTGGCCA AACGCTTCCG CCTACCAGGC ATCCGCCAGG GCTATGGCCT   421 GACAGAAACA ACCAACGCCG TCATGATCAC CCCCGAGGGG GACCGTAAGC CTGGCTCAGT   481 AGGCAAGGTG GTGCCCTTCT TCGAGGCTAA GGTGGTAGAC TTGGTCACCG GTAAGACACT   541 GGGTGTGAAC CAGCGCGGTG AGCTGTGCGT CCGTGGCCCC NTGATCATGA GCGGCTACGT   601 TAACNACCCC GAGGCTACNA ACGCTCTCNT CNACAANGAC GGCTGGNTGN ACAGCGGCGA   661 CNTCNCCTAC TGGGACNANG ANNANCACTT CTTCNTCNTG GNNCNGCNNN NNANCCTGAT   721 CNAATACNNN GGCTACCNNG TAGCCCCNNN CCNAACNGGA NGGNATNCTG NTGNNNCANC   781 CCTACNTCNN NNNNGCCNGA NTNNNCNNGN CTGNCCNANN ANNNNGNNNN NCNANCTGNC 841 CNCCNCNNNC NTCNNNNNNG ANNNNNNNNA AANCNNNNNN NNNN //
Primer: bGH-B_R (Pr0746) Region: insert mid(Akaluc mid) Sequence file: RDB15783_A8B8k.seq
>15783_15783_B2EeF_1_bGH-B_R_D02_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNC TTGCCGTCCT TCTTGGCCTT AGTGAGAATC    61 TCGCGGATCT TGCGGGCGTC TAACTTGCCG GTCGATCCTC TAGGGACTTC ATCCACAAAC   121 ACAACACCAC CGCGCAGCTT CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC   181 TCTTTCTCGG TCATGGTTTT ACCGTGTTCC AACACGACGA CTGCGGCGGG CAGCTCGCCG   241 GCATCGTCGT CGGGCAGGCC GGCGACTCCG GCGTCGAAGA TGTAGGGGTG TTGCAGCAGG   301 ATGCCCTCCA GTTCGGCTGG GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC   361 CGGTCCACGA TGAAGAAGTG CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC   421 CAGCCGTCCT TGTCGATGAG AGCGTTCGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG   481 ATCATGGGGC CACGGANNCN NNGCTCACCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG   541 ACCAAGTCTA CCACCTTAGC CTCGAAGAAG GGCACCACCT TGCCTACTGA GCCNNGCTTA   601 CNGTCCCCCT CGGGGGTGAT CNTGANNGCG TTGGTTGNTT CNGTCNNGNN NTANCCCTGG   661 CGGANGNCTG GNANGCNGAA NCGTTTGNNN NGNNNCNCNN CNNNNNGNNN ANNGGCNNCC 721 NNNNNGNNNN TNNNNNNCNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl