Resource data sheet
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pAAV2 TRE Venus-Akaluc

Expression vector of Akaluc luciferase.

Catalog number RDB15783
Resource name pAAV2 TRE Venus-Akaluc
Clone info. Expression vector of Akaluc luciferase.
This clone was constructed based on the pENTR1A-TRE-GFP-BGHpA vector (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009).
The following 2 commercial compounds have been demonstrated to be well catalyzed by Akaluc in vivo:
(1) TokeOni (AkaLumine-HCl), Sigma-Aldrich. Product No.: 808350.
(2) AkaLumine-HCl, Wako Pure Chemical Industry. Product No.: 012-26701, 018-26703.
Patent application PCT/JP2017/030491 submitted by RIKEN that covers the creation and use of Akaluc.
Vector backbone pAAV2-DEST(r) (Plasmid)
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Reference of nucleotide sequence LC320664 (DDBJ accession number)
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-TRE-GFP-BGHpA vector (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability
RDB15783 pAAV2 TRE Venus-Akaluc This clone has been withdrawn.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)

References

original Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
original Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15783_A8B8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Venus-C_F2 RDB15783_A8B8a.seq
>D04498K8_A8B8_1_Venus-C_F2_A02_01_ABI08.ab1
    1 AGAATTTGCA AGCCGCCGGG GATCACTCTC GGCATGGACG AGCTGTACAA GGGATCCATG
   61 GAAGATGCCA AAAACATTAA GAAGGGCCCA GCGCCGTTCT ACCCACTCGA AGACGGGACC
  121 GCCGGCGAGC AGCTGCACAA AGCCATGAAG CGCTACGCCC TGGTGCCCGG CGCCATCGCC
  181 TTTACCGACG CACATATTCA GGTGGACGTT ACCTACGCCG AGTACTTCGA GATGAGCGTT
  241 CGGCTGGCAG AAGCTATGAG GCGCTATGGG CTGAATACAA ACCATCGGAT CGTGGTGTGC
  301 AGCGAGAATA GCTCGCAGTT CTTCATGCCC GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT
  361 GTGGCCCCAG CTAACGACAT CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC
  421 CAGCCCACCG TCGTATTCGT GAGCAAGAAA GGGCTGCGAA AGGTCCTCAA CGTGCAAAAG
  481 AAGCTACCGA TCATACGAAA GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC
  541 CAAAGCATGT ACACCTTCGT GACTTCCCAT TTGCCACCCA GCTTCAACGA GTACGACTTC
  601 GTGCCCGAGA GCTTCGACCG GGACAAAACC ATCGCCCTGA TCATGAACAG TAGTGGTAGT
  661 ACAGGATTAC CCAAGGGCGT AGCCCTACCG CACCGCACCG CTTGTGTCCG ATTCAGTCAT
  721 GCCCGCGACC CCATCTTCGG CTACCAGAAC ATCCCCGACA CCGCTATCCT CAGCGTGGTG
  781 CCATTTCACC ACGGCTTCGG CATGTTCACC ACGCTGGGCT ACTTGATCTG CGGCTTTCGG
  841 GTCGTGCTCA TGTACCGCTT CGAGGAGGAG CTATTCTTGC GCAGCTTGCA AGACTATAAG
  901 ATTCAATCTG CCCTGCTGGT GCCCACACTA TTTAGCTGCC TCGCTAAGAG CACTCTCATC
  961 GACAAGTACG ACCTAAGCAG CTTGCGCGAG ATCGCCAAGC GGGCGGGGGC GGCCGCTTCA
 1021 GCAAGGGAGG GTAGGTGAGG GCCGTGGCCA AACGCTTCCG GCTACCAAGG CATCCGGCCC
1081 AGGGCTATGG GCCTGACAGA AACAACCAAC GGCCCGGTCT ATGTAAATCA C
//
Primer: Akaluc 1215-1243 RDB15783_A8B8b.seq
>D04498K8_A8B8_1_Akaluc 1215-1243_B02_02_ABI08.ab1
    1 GGCCAACGGG GGGCATCGCC CTACTGGGAC GAGGACGAGC ACTTCTTCAT CGTGGACCGG
   61 CTGAAGAGCC TGATCAAATA CAAGGGCTAC CAGGTAGCCC CAGCCGAACT GGAGGGCATC
  121 CTGCTGCAAC ACCCCTACAT CTTCGACGCC GGAGTCGCCG GCCTGCCCGA CGACGATGCC
  181 GGCGAGCTGC CCGCCGCAGT CGTCGTGTTG GAACACGGTA AAACCATGAC CGAGAAAGAG
  241 ATCGTGGACT ATGTGGCCAG CCAGGTTACA ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG
  301 TTTGTGGATG AAGTCCCTAG AGGATCGACC GGCAAGTTAG ACGCCCGCAA GATCCGCGAG
  361 ATTCTCACTA AGGCCAAGAA GGACGGCAAG ATCGCCGTGT AAACGCGTCT GTGCCTTCTA
  421 GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA
  481 CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC
  541 ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA
  601 GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGGCGGCCG CATCGATAAG CTTGTCGACG
  661 ATAAAGCCTG CTTTTTTGTA CAAACTTGTC ACGTGCGGAC CGAGCGGCCG CAGGAACCCC
721 TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTC
//
Primer: Akaluc 576-593 RDB15783_A8B8c.seq
>D04498K8_A8B8_1_Akaluc 576-593_C02_03_ABI08.ab1
    1 CGTTTACCCA AGGGGCGTAG CCCTACCGCA CCGCACCGCT TGTGTCCGAT TCAGTCATGC
   61 CCGCGACCCC ATCTTCGGCT ACCAGAACAT CCCCGACACC GCTATCCTCA GCGTGGTGCC
  121 ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT
  181 CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT
  241 TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTGCCTC GCTAAGAGCA CTCTCATCGA
  301 CAAGTACGAC CTAAGCAGCT TGCGCGAGAT CGCCAGCGGC GGGGCGCCGC TCAGCAAGGA
  361 GGTAGGTGAG GCCGTGGCCA AACGCTTCCG CCTACCAGGC ATCCGCCAGG GCTATGGCCT
  421 GACAGAAACA ACCAACGCCG TCATGATCAC CCCCGAGGGG GACCGTAAGC CTGGCTCAGT
  481 AGGCAAGGTG GTGCCCTTCT TCGAGGCTAA GGTGGTAGAC TTGGTCACCG GTAAGACACT
  541 GGGTGTGAAC CAGCGCGGTG AGCTGTGCGT CCGTGGCCCC ATGATCATGA GCGGCTACGT
  601 TAACAACCCC GAGGCTACGA ACGCTCTCAT CGACAAGGAC GGCTGGCTGC ACAGCGGCGA
  661 CATCGCCTAC TGGGACGAGG ACGAGCACTT CTTCATCGTG GACCGGCTGG AGAGCCTGAT
  721 CAAATACAAG GGCTACCAGG TAGCCCCAGC CCGAACTGGA GGGCATCCTG CTGCAACACC
  781 CCTACATCTT CTACGCCGGA GTCGCCCGGC CTGCCCGACG ACGATGCCCG GCGAGCTGCC
  841 CGCCGCAGTC GTCGTGTTGG AACACGGTTA AACCCATGAC CGAGAAAGAG ATCGTGGACT
  901 ATGTGGGCCA GCCCAGTTTA CAACCGCCAG AAGCTGCGCG CTGGTGTTGT GTTTTGTGAT
  961 GAAGTCCCTA GAGATCGACC CGGCCAAGTT AGACGCCCGC AGGATCCCCG AGATTTCTCA
 1021 CTTAAAGCCA GAAGAACGCC AGAATCGCCT GTTAAACCGT CTGTGCTTTC TAGTTGCAGC
1081 ATCTGTGTTG CCATCCCCGG GTGCTTTCCC CTA
//
Primer: Venus-N_R RDB15783_A8B8d.seq
>D04498K8_A8B8_1_Venus-N_R_D02_04_ABI08.ab1
    1 CTTTTCGGGG CCGGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACCAGGATGG
   61 GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT GGTGGCGGTA CCGAGCTCGA
  121 ATTCTCCAGG CGATCTGACG GTTCACTAAA CGAGCTCTGC TTATATAGGC CTCCCACCGT
  181 ACACGCCTAC CTCGACATAC GTTCTCTATC ACTGATAGGG AGTAAACTCG ACATACGTTC
  241 TCTATCACTG ATAGGGATAA ACTCGACATA CGTTCTCTAT CACTGATAGG GAGTAAACTC
  301 GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA TACGTTCTCT ATCACTGATA
  361 GGGAGTAAAC TCGACATCGT TCTCTATCAC TGATAGGGAG TAAACTCGAC ATACGTTCTC
  421 TATCACTGAT AGGGAGTAAA CTCGAGATAT CTAGACCCAG CTTTCTTGTA CAAAGTGGTA
481 CGCGTGCGGC CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
original Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1.
reference Nasu, Y., Unnaturally aglow with a bright inner light. Science 359 (6378): 868-869 (2018). PMID 29472468.
reference Kuchimaru, T., A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat. Commun. 7: 11856 (2016). PMID 27297211.

2019.03.20

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