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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV2 TRE Venus-Akaluc

Expression vector of Akaluc luciferase.

Catalog number RDB15783
Resource name pAAV2 TRE Venus-Akaluc
Clone info. Expression vector of Akaluc luciferase.
Comment This clone was constructed based on the pENTR1A-TRE-GFP-BGHpA vector (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009).
The following 2 commercial compounds have been demonstrated to be well catalyzed by Akaluc in vivo:
(1) TokeOni (AkaLumine-HCl), Sigma-Aldrich. Product No.: 808350.
(2) AkaLumine-HCl, Wako Pure Chemical Industry. Product No.: 012-26701, 018-26703.
Patent application PCT/JP2017/030491 submitted by RIKEN that covers the creation and use of Akaluc.
Vector backbone pAAV2-DEST(r) (Plasmid)
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Reference of insert sequence LC320664 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
firefly (Photinus pyralis) luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018).
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Additional terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-TRE-GFP-BGHpA vector (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested.
          The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018).
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          付加的提供条件 利用者は研究成果の公表にあたって、pENTR1A-TRE-GFP-BGHpA の文献 (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009) を引用し、順天堂大学日置寛之先生への謝辞の表明を必要とする。
          本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考 提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15783 pAAV2 TRE Venus-Akaluc DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
          original Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0081e (English text)
          Featured content Dnaconda's recommendation EXR0081j (Japanese text)
          Featured content Higher intensity luciferase (English text)
          Featured content Higher intensity luciferase (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15783_A8B8p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Venus-N_R
          Region: insert 5'(Venus 5',tight TRE pro),attB2,AAV2 ITR
          Sequence file: RDB15783_A8B8e.seq check
          >D04498K8_A8B8_1_Venus-N_R_D02_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNGGNCG TTNNNGTCGC CGTCCNGCTC GANCAGGNNG
             61 GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGTGGCGGT ACCGAGCTCG
            121 AATTCTCCAG GCGATCTGAC GGTTCACTAA ACGAGCTCTG CTTATATAGG CCTCCCACCG
            181 TACACGCCTA CCTCGACATA CGTTCTCTAT CACTGATAGG GAGTAAACTC GACATACGTT
            241 CTCTATCACT GATAGGGATA AACTCGACAT ACGTTCTCTA TCACTGATAG GGAGTAAACT
            301 CGACATACGT TCTCTATCAC TGATAGGGAG TAAACTCGAC ATACGTTCTC TATCACTGAT
            361 AGGGAGTAAA CTCGACATCG TTCTCTATCA CTGATAGGGA GTAAACTCGA CATACGTTCT
            421 CTATCACTGA TAGGGAGTAA ACTCGAGATA TCTAGACCCA GCTTTCTTGT ACAAAGTGGT
          481 ACGCGTGCGG CCGCAGGAAC CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTN
          //
          Primer: Akaluc 1215-1243
          Region: insert 3'(Akaluc 3',bGH pA),attB1 AAV2 ITR
          Sequence file: RDB15783_A8B8f.seq check
          >D04498K8_A8B8_1_Akaluc 1215-1243_B02_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNN NNNGNNNNNC NNTTCTTCNT CGTGGNNCGG
             61 CTGAAGAGCC TGATCAAATA CAAGGGCTAC CAGGTAGCCC CAGCCGAACT GGAGGGCATC
            121 CTGCTGCAAC ACCCCTACAT CTTCGACGCC GGAGTCGCCG GCCTGCCCGA CGACGATGCC
            181 GGCGAGCTGC CCGCCGCAGT CGTCGTGTTG GAACACGGTA AAACCATGAC CGAGAAAGAG
            241 ATCGTGGACT ATGTGGCCAG CCAGGTTACA ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG
            301 TTTGTGGATG AAGTCCCTAG AGGATCGACC GGCAAGTTAG ACGCCCGCAA GATCCGCGAG
            361 ATTCTCACTA AGGCCAAGAA GGACGGCAAG ATCGCCGTGT AAACGCGTCT GTGCCTTCTA
            421 GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA
            481 CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC
            541 ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA
            601 GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGGCGGCCG CATCGATAAG CTTGTCGACG
            661 ATAAAGCCTG CTTTTTTGTA CAAACTTGTC ACGTGCGGAC CGAGCGGCCG CAGGAACCCC
          721 TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGNN
          //
          Primer: D03160F1
          Region: insert mid(Venus 5')
          Sequence file: RDB15783_A8B8g.seq check
          >15783_15783_B2EeF_1_D03160F1_C02_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GAGAANTCGA GCTCGGTACC GCCACCATGG
             61 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
            121 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
            181 AGCTGACCCT GAAGCTGATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
            241 TGACCACCCT GGGCTACGGC CTGCAGTGCT TCGCCCGCTA CCCCGACCAC ATGAAGCAGC
            301 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
            361 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
            421 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
            481 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCACCGC CGACAAGCAG AAGAACGGCA
            541 TCAAGGCCAA CTTCAAGATC CGCCACAACA TCNAGGACGG CGGCGTGCAG CTCGCCGACN
            601 ACTACCANCA GANCACCCCC NTCGGCGANN GNCCCGTGCT GCTGCCCGAC AACCACTANC
            661 TGANCTACCA GTCCNNCCNN ANNNAAGANC CCNANGANNA NNNNNNTNNN NNNGNNCNNG
          721 CNNNNNNNNN N
          //
          Primer: pGL4ins_R
          Region: insert mid(Akaluc 5',Venus 3')
          Sequence file: RDB15783_A8B8h.seq check
          >15783_15783_B2EeF_1_pGL4ins_R_B02_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNAANG GCGCTGGGCC CTTCTTAATG TTTTTGGCAT
             61 CTTCCATGGA TCCCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA
            121 ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGT
            181 AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT
            241 GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTGG
            301 CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG TTGTAGTTGT
            361 ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA
            421 TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT
            481 CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA
            541 AGTCGTGCTG CTTCATGTGN NNNGGGTAGC GGGCGAANCA CTGCAGGCCG TANCCCAGGG
            601 TGGTCACGAG GGTGGGCCAG GGCNNNGGCA GCTTGCCGGN GGNGCANATC NNCTTCNNGG
            661 TCNNCTTGNC NGTNNNGGCN TCNNCCTCGC CCNCNCCGGA NNCNCNNAAN TTGNNGNNNT
          721 TNNNNTCNCC NNCNNNTNNA NCNNNNNGGG NNNNNNNNNG GNNNNNNNNN N
          //
          Primer: Venus-C_F2
          Region: insert mid(Akaluc mid)
          Sequence file: RDB15783_A8B8i.seq check
          >D04498K8_A8B8_1_Venus-C_F2_A02_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCTC NNNNNGGNNG ANCTGTACNA GGGNNCCNTG
             61 GAAGANGCCN AAAACATTAA GAAGGGCCCA GCGCCGTTCT ACCCACTCGA AGACGGGACC
            121 GCCGGCGAGC AGCTGCACAA AGCCATGAAG CGCTACGCCC TGGTGCCCGG CGCCATCGCC
            181 TTTACCGACG CACATATTCA GGTGGACGTT ACCTACGCCG AGTACTTCGA GATGAGCGTT
            241 CGGCTGGCAG AAGCTATGAG GCGCTATGGG CTGAATACAA ACCATCGGAT CGTGGTGTGC
            301 AGCGAGAATA GCTCGCAGTT CTTCATGCCC GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT
            361 GTGGCCCCAG CTAACGACAT CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC
            421 CAGCCCACCG TCGTATTCGT GAGCAAGAAA GGGCTGCGAA AGGTCCTCAA CGTGCAAAAG
            481 AAGCTACCGA TCATACGAAA GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC
            541 CAAAGCATGT ACACCTTCGT GACTTCCCAT TTGCCACCCA GCTTCAACGA GTACGACTTC
            601 GTGCCCGAGA GCTTCGACCG GGACAAAACC ATCGCCCTGA TCATGAACAG TAGTGGTAGT
            661 ACAGGATTAC CCAAGGGCGT AGCCCTACCG CACCGCACCG CTTGTGTCCG ATTCAGTCAT
            721 GCCCGCGACC CCATCTTCGG CTACCAGAAC ATCCCCGACA CCGCTATCCT CAGCGTGGTG
            781 CCATTTCACC ACGGCTTCNG CATGTTCACC ACGCTGGGCT ACTTGATCTG CGGCTTTCGG
            841 GNCGNGCTCA TGTANCGCTT NNNNGAGGAN CTATTCTTGC GCAGCTNGCN AGANTATAAG
            901 ANNCAATCTG CCNNGCNNNN GCCCACACTA TTTANCTGCN NCGCTAAGAN CNNTNTCATC
          961 NACAANNNCN NNCNNNNNNG CTNGCNCGAN ANNNNNNNNN N
          //
          Primer: Akaluc 576-593
          Region: insert mid(Akaluc mid)
          Sequence file: RDB15783_A8B8j.seq check
          >D04498K8_A8B8_1_Akaluc 576-593_C02_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNCCGCN NCNNNNNNCT TGTGTNNNNN NCNGTCATGC
             61 CCGCGACCCC ATCTTCGGCT ACCAGAACAT CCCCGACACC GCTATCCTCA GCGTGGTGCC
            121 ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT
            181 CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT
            241 TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTGCCTC GCTAAGAGCA CTCTCATCGA
            301 CAAGTACGAC CTAAGCAGCT TGCGCGAGAT CGCCAGCGGC GGGGCGCCGC TCAGCAAGGA
            361 GGTAGGTGAG GCCGTGGCCA AACGCTTCCG CCTACCAGGC ATCCGCCAGG GCTATGGCCT
            421 GACAGAAACA ACCAACGCCG TCATGATCAC CCCCGAGGGG GACCGTAAGC CTGGCTCAGT
            481 AGGCAAGGTG GTGCCCTTCT TCGAGGCTAA GGTGGTAGAC TTGGTCACCG GTAAGACACT
            541 GGGTGTGAAC CAGCGCGGTG AGCTGTGCGT CCGTGGCCCC NTGATCATGA GCGGCTACGT
            601 TAACNACCCC GAGGCTACNA ACGCTCTCNT CNACAANGAC GGCTGGNTGN ACAGCGGCGA
            661 CNTCNCCTAC TGGGACNANG ANNANCACTT CTTCNTCNTG GNNCNGCNNN NNANCCTGAT
            721 CNAATACNNN GGCTACCNNG TAGCCCCNNN CCNAACNGGA NGGNATNCTG NTGNNNCANC
            781 CCTACNTCNN NNNNGCCNGA NTNNNCNNGN CTGNCCNANN ANNNNGNNNN NCNANCTGNC
          841 CNCCNCNNNC NTCNNNNNNG ANNNNNNNNA AANCNNNNNN NNNN
          //
          Primer: bGH-B_R
          Region: insert mid(Akaluc mid)
          Sequence file: RDB15783_A8B8k.seq check
          >15783_15783_B2EeF_1_bGH-B_R_D02_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNC TTGCCGTCCT TCTTGGCCTT AGTGAGAATC
             61 TCGCGGATCT TGCGGGCGTC TAACTTGCCG GTCGATCCTC TAGGGACTTC ATCCACAAAC
            121 ACAACACCAC CGCGCAGCTT CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC
            181 TCTTTCTCGG TCATGGTTTT ACCGTGTTCC AACACGACGA CTGCGGCGGG CAGCTCGCCG
            241 GCATCGTCGT CGGGCAGGCC GGCGACTCCG GCGTCGAAGA TGTAGGGGTG TTGCAGCAGG
            301 ATGCCCTCCA GTTCGGCTGG GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC
            361 CGGTCCACGA TGAAGAAGTG CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC
            421 CAGCCGTCCT TGTCGATGAG AGCGTTCGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG
            481 ATCATGGGGC CACGGANNCN NNGCTCACCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG
            541 ACCAAGTCTA CCACCTTAGC CTCGAAGAAG GGCACCACCT TGCCTACTGA GCCNNGCTTA
            601 CNGTCCCCCT CGGGGGTGAT CNTGANNGCG TTGGTTGNTT CNGTCNNGNN NTANCCCTGG
            661 CGGANGNCTG GNANGCNGAA NCGTTTGNNN NGNNNCNCNN CNNNNNGNNN ANNGGCNNCC
          721 NNNNNGNNNN TNNNNNNCNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
          original Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1.
          reference Nasu, Y., Unnaturally aglow with a bright inner light. Science 359 (6378): 868-869 (2018). PMID 29472468.
          reference Kuchimaru, T., A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat. Commun. 7: 11856 (2016). PMID 27297211.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl