RDB15783 - pAAV2 TRE Venus-Akaluc

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pAAV2 TRE Venus-Akaluc (#RDB15783)

Expression vector of Akaluc luciferase.

Clone info.	Expression vector of Akaluc luciferase.
Comment	This clone was constructed based on the pENTR1A-TRE-GFP-BGHpA vector (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009). The following 2 commercial compounds have been demonstrated to be well catalyzed by Akaluc in vivo: (1) TokeOni (AkaLumine-HCl), Sigma-Aldrich. Product No.: 808350. (2) AkaLumine-HCl, Wako Pure Chemical Industry. Product No.: 012-26701, 018-26703. Patent application PCT/JP2017/030491 submitted by RIKEN that covers the creation and use of Akaluc.
Vector backbone	pAAV2-DEST(r) (RDB15111) (Plasmid)
Gene/insert name	firefly (Photinus pyralis) luciferase cDNA
Reference of insert sequence	LC320664 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

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Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Iwano, S. et al., Science 359 (6378): 935-939 (2018).
Additional terms and conditions:
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-TRE-GFP-BGHpA vector (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Iwano, S. et al., Science 359 (6378): 935-939 (2018). Additional terms and conditions: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-TRE-GFP-BGHpA vector (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Iwano, S. et al., Science 359 (6378): 935-939 (2018). 付加的使用条件：利用者は研究成果の公表にあたって、pENTR1A-TRE-GFP-BGHpA の文献 (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009) を引用し、順天堂大学日置寛之先生への謝辞の表明を必要とする。本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
備考	提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15783	pAAV2 TRE Venus-Akaluc	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

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How to cite this biological resource

Materials & Methods section:

The pAAV2 TRE Venus-Akaluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15783).

Reference section:

Iwano, S., Sugiyama, M., Hama, H., Watakabe, A., Hasegawa, N., Kuchimaru, T., Tanaka, K.Z., Takahashi, M., Ishida, Y., Hata, J., Shimozono, S., Namiki, K., Fukano, T., Kiyama, M., Okano, H., Kizaka-Kondoh, S., McHugh, T.J., Yamamori, T., Hioki, H., Maki, S., Miyawaki, A., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15783_A8B8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Venus-N_R (Pr0341) Region: insert 5'(Venus 5',tight TRE pro),attB2,AAV2 ITR Sequence file: RDB15783_A8B8e.seq
>D04498K8_A8B8_1_Venus-N_R_D02_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGGNCG TTNNNGTCGC CGTCCNGCTC GANCAGGNNG 61 GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGTGGCGGT ACCGAGCTCG 121 AATTCTCCAG GCGATCTGAC GGTTCACTAA ACGAGCTCTG CTTATATAGG CCTCCCACCG 181 TACACGCCTA CCTCGACATA CGTTCTCTAT CACTGATAGG GAGTAAACTC GACATACGTT 241 CTCTATCACT GATAGGGATA AACTCGACAT ACGTTCTCTA TCACTGATAG GGAGTAAACT 301 CGACATACGT TCTCTATCAC TGATAGGGAG TAAACTCGAC ATACGTTCTC TATCACTGAT 361 AGGGAGTAAA CTCGACATCG TTCTCTATCA CTGATAGGGA GTAAACTCGA CATACGTTCT 421 CTATCACTGA TAGGGAGTAA ACTCGAGATA TCTAGACCCA GCTTTCTTGT ACAAAGTGGT 481 ACGCGTGCGG CCGCAGGAAC CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTN //
Primer: Akaluc 1215-1243 (Pr0540) Region: insert 3'(Akaluc 3',bGH pA),attB1 AAV2 ITR Sequence file: RDB15783_A8B8f.seq
>D04498K8_A8B8_1_Akaluc 1215-1243_B02_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNN NNNGNNNNNC NNTTCTTCNT CGTGGNNCGG 61 CTGAAGAGCC TGATCAAATA CAAGGGCTAC CAGGTAGCCC CAGCCGAACT GGAGGGCATC 121 CTGCTGCAAC ACCCCTACAT CTTCGACGCC GGAGTCGCCG GCCTGCCCGA CGACGATGCC 181 GGCGAGCTGC CCGCCGCAGT CGTCGTGTTG GAACACGGTA AAACCATGAC CGAGAAAGAG 241 ATCGTGGACT ATGTGGCCAG CCAGGTTACA ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG 301 TTTGTGGATG AAGTCCCTAG AGGATCGACC GGCAAGTTAG ACGCCCGCAA GATCCGCGAG 361 ATTCTCACTA AGGCCAAGAA GGACGGCAAG ATCGCCGTGT AAACGCGTCT GTGCCTTCTA 421 GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA 481 CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC 541 ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA 601 GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGGCGGCCG CATCGATAAG CTTGTCGACG 661 ATAAAGCCTG CTTTTTTGTA CAAACTTGTC ACGTGCGGAC CGAGCGGCCG CAGGAACCCC 721 TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGNN //
Primer: D03160F1 (Pr0357) Region: insert mid(Venus 5') Sequence file: RDB15783_A8B8g.seq
>15783_15783_B2EeF_1_D03160F1_C02_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GAGAANTCGA GCTCGGTACC GCCACCATGG 61 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG 121 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA 181 AGCTGACCCT GAAGCTGATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG 241 TGACCACCCT GGGCTACGGC CTGCAGTGCT TCGCCCGCTA CCCCGACCAC ATGAAGCAGC 301 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA 361 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA 421 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC 481 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCACCGC CGACAAGCAG AAGAACGGCA 541 TCAAGGCCAA CTTCAAGATC CGCCACAACA TCNAGGACGG CGGCGTGCAG CTCGCCGACN 601 ACTACCANCA GANCACCCCC NTCGGCGANN GNCCCGTGCT GCTGCCCGAC AACCACTANC 661 TGANCTACCA GTCCNNCCNN ANNNAAGANC CCNANGANNA NNNNNNTNNN NNNGNNCNNG 721 CNNNNNNNNN N //
Primer: pGL4ins_R (Pr0666) Region: insert mid(Akaluc 5',Venus 3') Sequence file: RDB15783_A8B8h.seq
>15783_15783_B2EeF_1_pGL4ins_R_B02_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNAANG GCGCTGGGCC CTTCTTAATG TTTTTGGCAT 61 CTTCCATGGA TCCCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA 121 ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGT 181 AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT 241 GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTGG 301 CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG TTGTAGTTGT 361 ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA 421 TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT 481 CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA 541 AGTCGTGCTG CTTCATGTGN NNNGGGTAGC GGGCGAANCA CTGCAGGCCG TANCCCAGGG 601 TGGTCACGAG GGTGGGCCAG GGCNNNGGCA GCTTGCCGGN GGNGCANATC NNCTTCNNGG 661 TCNNCTTGNC NGTNNNGGCN TCNNCCTCGC CCNCNCCGGA NNCNCNNAAN TTGNNGNNNT 721 TNNNNTCNCC NNCNNNTNNA NCNNNNNGGG NNNNNNNNNG GNNNNNNNNN N //
Primer: Venus-C_F2 (Pr0454) Region: insert mid(Akaluc mid) Sequence file: RDB15783_A8B8i.seq
>D04498K8_A8B8_1_Venus-C_F2_A02_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCTC NNNNNGGNNG ANCTGTACNA GGGNNCCNTG 61 GAAGANGCCN AAAACATTAA GAAGGGCCCA GCGCCGTTCT ACCCACTCGA AGACGGGACC 121 GCCGGCGAGC AGCTGCACAA AGCCATGAAG CGCTACGCCC TGGTGCCCGG CGCCATCGCC 181 TTTACCGACG CACATATTCA GGTGGACGTT ACCTACGCCG AGTACTTCGA GATGAGCGTT 241 CGGCTGGCAG AAGCTATGAG GCGCTATGGG CTGAATACAA ACCATCGGAT CGTGGTGTGC 301 AGCGAGAATA GCTCGCAGTT CTTCATGCCC GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT 361 GTGGCCCCAG CTAACGACAT CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC 421 CAGCCCACCG TCGTATTCGT GAGCAAGAAA GGGCTGCGAA AGGTCCTCAA CGTGCAAAAG 481 AAGCTACCGA TCATACGAAA GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC 541 CAAAGCATGT ACACCTTCGT GACTTCCCAT TTGCCACCCA GCTTCAACGA GTACGACTTC 601 GTGCCCGAGA GCTTCGACCG GGACAAAACC ATCGCCCTGA TCATGAACAG TAGTGGTAGT 661 ACAGGATTAC CCAAGGGCGT AGCCCTACCG CACCGCACCG CTTGTGTCCG ATTCAGTCAT 721 GCCCGCGACC CCATCTTCGG CTACCAGAAC ATCCCCGACA CCGCTATCCT CAGCGTGGTG 781 CCATTTCACC ACGGCTTCNG CATGTTCACC ACGCTGGGCT ACTTGATCTG CGGCTTTCGG 841 GNCGNGCTCA TGTANCGCTT NNNNGAGGAN CTATTCTTGC GCAGCTNGCN AGANTATAAG 901 ANNCAATCTG CCNNGCNNNN GCCCACACTA TTTANCTGCN NCGCTAAGAN CNNTNTCATC 961 NACAANNNCN NNCNNNNNNG CTNGCNCGAN ANNNNNNNNN N //
Primer: Akaluc 576-593 (Pr0539) Region: insert mid(Akaluc mid) Sequence file: RDB15783_A8B8j.seq
>D04498K8_A8B8_1_Akaluc 576-593_C02_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNCCGCN NCNNNNNNCT TGTGTNNNNN NCNGTCATGC 61 CCGCGACCCC ATCTTCGGCT ACCAGAACAT CCCCGACACC GCTATCCTCA GCGTGGTGCC 121 ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT 181 CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT 241 TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTGCCTC GCTAAGAGCA CTCTCATCGA 301 CAAGTACGAC CTAAGCAGCT TGCGCGAGAT CGCCAGCGGC GGGGCGCCGC TCAGCAAGGA 361 GGTAGGTGAG GCCGTGGCCA AACGCTTCCG CCTACCAGGC ATCCGCCAGG GCTATGGCCT 421 GACAGAAACA ACCAACGCCG TCATGATCAC CCCCGAGGGG GACCGTAAGC CTGGCTCAGT 481 AGGCAAGGTG GTGCCCTTCT TCGAGGCTAA GGTGGTAGAC TTGGTCACCG GTAAGACACT 541 GGGTGTGAAC CAGCGCGGTG AGCTGTGCGT CCGTGGCCCC NTGATCATGA GCGGCTACGT 601 TAACNACCCC GAGGCTACNA ACGCTCTCNT CNACAANGAC GGCTGGNTGN ACAGCGGCGA 661 CNTCNCCTAC TGGGACNANG ANNANCACTT CTTCNTCNTG GNNCNGCNNN NNANCCTGAT 721 CNAATACNNN GGCTACCNNG TAGCCCCNNN CCNAACNGGA NGGNATNCTG NTGNNNCANC 781 CCTACNTCNN NNNNGCCNGA NTNNNCNNGN CTGNCCNANN ANNNNGNNNN NCNANCTGNC 841 CNCCNCNNNC NTCNNNNNNG ANNNNNNNNA AANCNNNNNN NNNN //
Primer: bGH-B_R (Pr0746) Region: insert mid(Akaluc mid) Sequence file: RDB15783_A8B8k.seq
>15783_15783_B2EeF_1_bGH-B_R_D02_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNC TTGCCGTCCT TCTTGGCCTT AGTGAGAATC 61 TCGCGGATCT TGCGGGCGTC TAACTTGCCG GTCGATCCTC TAGGGACTTC ATCCACAAAC 121 ACAACACCAC CGCGCAGCTT CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC 181 TCTTTCTCGG TCATGGTTTT ACCGTGTTCC AACACGACGA CTGCGGCGGG CAGCTCGCCG 241 GCATCGTCGT CGGGCAGGCC GGCGACTCCG GCGTCGAAGA TGTAGGGGTG TTGCAGCAGG 301 ATGCCCTCCA GTTCGGCTGG GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC 361 CGGTCCACGA TGAAGAAGTG CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC 421 CAGCCGTCCT TGTCGATGAG AGCGTTCGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG 481 ATCATGGGGC CACGGANNCN NNGCTCACCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG 541 ACCAAGTCTA CCACCTTAGC CTCGAAGAAG GGCACCACCT TGCCTACTGA GCCNNGCTTA 601 CNGTCCCCCT CGGGGGTGAT CNTGANNGCG TTGGTTGNTT CNGTCNNGNN NTANCCCTGG 661 CGGANGNCTG GNANGCNGAA NCGTTTGNNN NGNNNCNCNN CNNNNNGNNN ANNGGCNNCC 721 NNNNNGNNNN TNNNNNNCNN NNNNNNNN //

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References

Original, user report and related articles

original	Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486. [link to RRC of NBRP]
reference	Nasu, Y., Unnaturally aglow with a bright inner light. Science 359 (6378): 868-869 (2018). PMID 29472468.
reference	Kuchimaru, T., A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat. Commun. 7: 11856 (2016). PMID 27297211.