Resource data sheet
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pAAV2 TRE Venus-Akaluc (#RDB15783)

Expression vector of Akaluc luciferase.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Iwano, S., Science 359 (6378): 935-939 (2018). PMID 29472486. [PubMed] [Article] [RRC of NBRP]
Publication Miyawaki, A., Patent publication WO 2018/038248 A1. [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of Akaluc luciferase.
Comment This clone was constructed based on the pENTR1A-TRE-GFP-BGHpA vector (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009).
The following 2 commercial compounds have been demonstrated to be well catalyzed by Akaluc in vivo:
(1) TokeOni (AkaLumine-HCl), Sigma-Aldrich. Product No.: 808350.
(2) AkaLumine-HCl, Wako Pure Chemical Industry. Product No.: 012-26701, 018-26703.
Patent application PCT/JP2017/030491 submitted by RIKEN that covers the creation and use of Akaluc.
Vector backbone pAAV2-DEST(r) (RDB15111) (Plasmid)
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Reference of insert sequence LC320664 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Iwano, S. et al., Science 359 (6378): 935-939 (2018).
Additional terms and conditions:
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-TRE-GFP-BGHpA vector (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Iwano, S. et al., Science 359 (6378): 935-939 (2018).
付加的使用条件：
利用者は研究成果の公表にあたって、pENTR1A-TRE-GFP-BGHpA の文献 (Hioki, H. et al., Neurosci Res. 63(2): 149-154, 2009) を引用し、順天堂大学日置寛之先生への謝辞の表明を必要とする。
本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee
RDB15783 pAAV2 TRE Venus-Akaluc DNA solution

How to cite this biological resource

Materials & Methods section:

The pAAV2 TRE Venus-Akaluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15783).

Reference section:

Iwano, S., Sugiyama, M., Hama, H., Watakabe, A., Hasegawa, N., Kuchimaru, T., Tanaka, K.Z., Takahashi, M., Ishida, Y., Hata, J., Shimozono, S., Namiki, K., Fukano, T., Kiyama, M., Okano, H., Kizaka-Kondoh, S., McHugh, T.J., Yamamori, T., Hioki, H., Maki, S., Miyawaki, A., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486. [PubMed] [Article] [RRC of NBRP]
Miyawaki, A., Iwano, S., Modified luciferase. Patent publication WO 2018/038248 A1. [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486. [PubMed] [Article] [RRC of NBRP]
original Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1. [RRC of NBRP]
reference Nasu, Y., Unnaturally aglow with a bright inner light. Science 359 (6378): 868-869 (2018). PMID 29472468. [PubMed] [Article]
reference Kuchimaru, T., A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat. Commun. 7: 11856 (2016). PMID 27297211. [PubMed] [Article]