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mAG-hGeminin(1/110) / pCSII-EF

Lentivirus vector plasmid of mAG-hGeminin(1/110). Component of Fucci cell cycle probe.

Catalog number RDB15268
Resource name mAG-hGeminin(1/110) / pCSII-EF
Alternative name FUCCI-Green
Clone info. Lentivirus vector plasmid of mAG-hGeminin(1/110). One of the component of Fucci cell cycle probe. Fluorescence was detected in the nucleus.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (Plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human Geminin cDNA Galaxea fascicularis GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human Geminin Galaxea fascicularis GFP

          Reference sequence
            
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
          Ordering Please visit Ordering instruction.[link] 
          Order form 
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          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 【追加提供依頼書】受入れ確認書(書式J)
          このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          提供条件は英文のまま記載してください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15268 mAG-hGeminin(1/110) / pCSII-EF DNA solution

          Ordering instruction of plasmids [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15268_A7H3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Region: EF1a pro,insert5'
          Sequence file: RDB15268_A7H3a.seq check
          >D04498H8_2_A7H3_EF1a-C_F_B01_04_ABI24.ab1
              1 TCATGTGGAT TTGCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG
             61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAACACG CTACCGGTCT CGAGAATTCG
            121 CCACCATGGT GAGCGTGATC AAGCCCGAGA TGAAGATCAA GCTGTGCATG AGGGGCACCG
            181 TGAACGGCCA CAACTTCGTG ATCGAGGGCG AGGGCAAGGG CAACCCCTAC GAGGGCACCC
            241 AGATCCTGGA CCTGAACGTG ACCGAGGGCG CCCCCCTGCC CTTCGCCTAC GACATCCTGA
            301 CCACCGTGTT CCAGTACGGC AACAGGGCCT TCACCAAGTA CCCCGCCGAC ATCCAGGACT
            361 ACTTCAAGCA GACCTTCCCC GAGGGCTACC ACTGGGAGAG GAGCATGACC TACGAGGACC
            421 AGGGCATCTG CACCGCCACC AGCAACATCA GCATGAGGGG CGACTGCTTC TTCTACGACA
            481 TCAGGTTCGA CGGCACCAAC TTCCCCCCCA ACGGCCCCGT GATGCAGAAG AAGACCCTGA
            541 AGTGGGAGCC CAGCACCGAG AAGATGTACG TGGAGGACGG CGTGCTGAAG GGCGACGTGA
            601 ACATGAGGCT GCTGCTGGAG GGCGGCGGCC ACTACAGGTG CGACTTCAAG ACCACCTACA
            661 AGGCCAAGAA GGAGGTGAGG CTGCCCGACG CCCACAAGAT CGACCACAGG ATCGAGATCC
            721 TGAAGCACGA CAAGGACTAC AACAAGGTGA AGCTGTACGA GAACGCCGTG GCCAGGTACT
            781 CCATGCTGCC CAGCCAGGCC AAGGGATATC CATCACACTG GCGGCCGCTC GAGATGAATC
            841 CCAGTATGAA GCAGAAACAA GAAGAAATCA AAGAGAATAT AAAGAATAGT TCTGTCCCAA
            901 GAAGAACTCT GAAGATGATT CAGCCTTCTG CATCTGGATC TCTTGTTGGA AGAGAAAATG
            961 AGCTGTCCGC AGCTTGTCCA AAAGGAACAT CGGGAATGAC CACTTAACAT CTACAACTTC
           1021 CAGCCCTGGG GGTTATTGTC CAGAATCTAG TGAAAATAAA AATCTTGAGA GTCACCCAGG
           1081 AGTTCATTGA TCCTATGATT AAAGAAAAAT CCAATCGTCT CAGTAATGGA GGAAGTGCAG
          1141 GAAAACGGGA GAAAAGGCCG CCTGTTAATC A
          //
          Primer: PRE_rev
          Region: PRE,insert3'
          Sequence file: RDB15268_A7H3f.seq check
          >D04498H8-2_A7H3_PRE_rev_B02_05_ABI24.ab1
              1 ACGAATCCAA ATAAGCGTAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA
             61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
            121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT
            181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT
            241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC
            301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG
            361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT
            421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC
            481 CCTTGGCCTG GCTGGGCAGC ATGGAGTACC TGGCCACGGC GTTCTCGTAC AGCTTCACCT
            541 TGTTGTAGTC CTTGTCGTGC TTCAGGATCT CGATCCTGTG GTCGATCTTG TGGGCGTCGG
            601 GCAGCCTCAC CTCCTTCTTG GCCTTGTAGG TGGTCTTGAA GTCGCACCTG TAGTGGCCGC
            661 CGCCCTCCAG CAGCAGCCTC ATGTTCACGT CGCCCTTCAG CACGCCGTCC TCCACGTACA
            721 TCTTCTCGGT GCTGGGCTCC CACTTCAGGG TCTTCTTCTG CATCACGGGG CCGTTGGGGG
            781 GGAAGTTGGT GCCGTCGAAC CTGATGTCGT AGAAGAAGCA GTCGCCCCTC ATGCTGATGT
            841 TGCTGGTGGC GGTGCAGATG CCCTGGTCCT CGTAGGTCAT GCTCCTCTCC CAGTGGTAGC
            901 CCTCGGGGAA GGTCTGCTTG AAGTAGTCCT GGATGTCGGC GGGGTACTTG GTGAAGGCCC
            961 TGTTGCCGTA CTGGAACACG GTGGTCAGGA TGTCGTAGGC GAAGGACAAG GGGGGCGCCC
           1021 TCGGTCCACG TTCAAGGTCC AGGATCTGGG TGCCCTCGTA AGGGGTTGCC CTTGCCCTTC
           1081 GCCTCGAATC ACGAAGTTGT GGCGATCACG GGTGGCCCCC TCATGCCACA GACTGAATCT
           1141 CATCTTCGGG CTGGATCCAG CGCTCACGAT GGGTGGCGAA TTCTCTGCAA GGAAACCGGC
          1201 GTATGACCG
          //
          Primer: CMV_Forward
          Region: CMV pro,5'LTR,HIV-1 psi
          Sequence file: RDB15268_A7H3c.seq check
          >D04498H8-2_A7H3_CMV-Forward_A09_03_ABI24.ab1
              1 TTGTTACAAC TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
            121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
            181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
            241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG
            301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC
            361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA
            421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT
            481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG
            541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA
            601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT
            661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA
            721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG
            781 CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA
          841 ATATAAAGTA GTA
          //
          Primer: cPPT_CTS-E_R
          Region: cPPT_CTS,RRE
          Sequence file: RDB15268_A7H3g.seq check
          >15268_15268_B0B4F_cPPT_CTS-E_R_F02_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCT TTAGTTTGTA TGTCTGTTGC TATTATGTCT
             61 ACTATTCTTT CCCCTGCACT GTACCCCCCA ATCCCCCCTT TTCTTTTAAA ATTGTGGATG
            121 AATACTGCCA TTTGTAGATC CGTTCACTAA TCGAATGGAT CTGTCTCTGT CTCTCTCTCC
            181 ACCTTCTTCT TCTATTCCTT CGGGCCTGTC GGGTCCCCTC GGGGTTGGGA GGTGGGTCTG
            241 AAACGATAAT GGTGAATATC CCTGCCTAAC TCTATTCACT ATAGAAAGTA CAGCAAAAAC
            301 TATTCTTAAA CCTACCAAGC CTCCTACTAT CATTATGAAT AATTTTATAT ACCACAGCCA
            361 ATTTGTTATG TTAAACCAAT TCCACAAACT TGCCCATTTA TCTAATTCCA ATAATTCTTG
            421 TTCATTCTTT TCTTGCTGGT TTTGCGATTC TTCAATTAAG GAGTGTATTA AGCTTGTGTA
            481 ATTGTTAATT TCTCTGTCCC ACTCCATCCA GGTCGTGTGA TTCCAAATCT GTTCCAGAGA
            541 TTTATTACTC CAACTAGCAT TCCAAGGCAC AGCAGTGGTG CAAATGAGTT TTCCAGAGCA
            601 ACCCCAAATC CCCAGGAGCT GTTGATCCTT TAGGTATCTT TCCACAGCCA GGATTCTTGC
            661 CTGGAGCTGC TTGATGCCCC AGACTGTGAG TTGCAACAGA TGCTGTTGCG CCTCAATAGC
            721 CCTCAGCAAA TTGTTCTGCT GCTGCACTAT ANCAGACAAT AATTGTCTGG CCTGTACCGT
            781 CNGCGTCATT GANGCTGCGC CCATAGTGCT TCCTGCTGCT CCCNAGAACN CAAGGANCAA
            841 AGCTNCTATT NCNACNGCTC TTTTTTTCTC TCTGCNNNAC TCNNNTCTTN GCCTTNNNGG
            901 GNGCNACNNC TNANGGTNCN NTTTTTACTA CTTTATATTT ATATNANNCA CTTCTCNNAN
          961 NNNNCCNNNA NATNNNCNNN NNNNNNN
          //
          Primer: BGH_rev2
          Region: bGHp(A),3'LTR,PRE
          Sequence file: RDB15268_A7H3d.seq check
          >D04498H8-2_A7H3_BGH_rev2_B04_04_ABI24.ab1
              1 CTTGCAGCGG TCAGTAAGCA CGGGGGAGGG GCAAGCAACA GATGGCTGGC AACTAGAAGG
             61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
            121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
            181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
            241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
            301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
            361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
            421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
            481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
            541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
            601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
            661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
            721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
            781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGAGGGCGAA
            841 GGCGAAGACG CGGAAGAGGC CGCAGAGCCG GCAGCAGGCC GCGGGAAAGG AAGGTCCGCT
            901 GGATTGAGGG CCGAAAGGGA CGTAGCAGAA GGACGTCCCG CGCAGAATCC AGGTGGGCAA
            961 CACAGGCGAG CAGCCATGGG AAAAGGACGT CAGCTTTCCC CGACAACACC ACGGGAATTG
           1021 TCAGTGCCCA CAGCCGAGCC CCTGTCCAGC AGCGGGGCAG CAGCGCGATG AGGTCCGTCG
           1081 TGGCATAGGA GGGAAGCGAA AGTCTGGGGA GCTGACAGTG GTGCCATGGC CCGACCAATG
          1141 GGGGGTTGGC GC
          //
          Primer: SV40pro_F
          Region: SV40 pro_ori,Zeo,SV40p(A)
          Sequence file: RDB15268_A7H3e.seq check
          >D04498H8-2_A7H3_SV40pro_F_B05_05_ABI24.ab1
              1 CCCCGGTCAC GGGACTTTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
             61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
            121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
            181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT
            241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT
            301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC
            361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT
            421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG
            481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA
            541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
            601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
            661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
            721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
            781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC
            841 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
            901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
            961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
           1021 TTTAATGATC GGCCAACGCG CGGGGAGAGG CGTTTTGCGT ATTGGGGCGC TTCTTCGGCT
           1081 TCTCGCTCAC TGACTCGCTG CGCTCGGTCG TCCGGCCTGC GGCGAGCCGT ATCAGCTTCA
          1141 CTCAGCGTTA GTACGGCTTA TCTCCCACC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078.
          reference Singer, D., Prominin-1 controls stem cell activation by orchestrating ciliary dynamics. EMBO J. 38 (2). pii: e99845 (2019). PMID 30523147.
          reference Tan, Z., Mammalian Cells Undergo Endoreduplication in Response to Lactic Acidosis. Sci Rep. 8 (1): 2890 (2018). PMID 29440645.
          reference Takahashi, H., Significance of Polypyrimidine Tract-Binding Protein 1 Expression in Colorectal Cancer. Mol. Cancer. Ther. 14 (7): 1705-1716 (2015). PMID 25904505.
          reference Kagawa, Y., Cell Cycle-Dependent Rho GTPase Activity Dynamically Regulates Cancer Cell Motility and Invasion In Vivo. PLoS One, 8 (12): e83629 (2013). PMID 24386239.
          user_report Onozato, Y., Radiosensitivity of quiescent and proliferating cells grown as multicellular tumor spheroids. Cancer Sci. 108 (4): 704-712 (2017). PMID 28135769.
          user_report Sereti, K.I., Methods to study the proliferation and differentiation of cardiac side population (CSP) cells. Methods Mol. Biol. 1036: 95-106 (2013). PMID 23807790.

          2022.05.17

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