Resource data sheet
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mAG-hGeminin(1/110) / pCSII-EF

Lentivirus vector construct of mAG-hGeminin(1/110). Component of Fucci cell cycle probe.

Catalog number RDB15268
Resource name mAG-hGeminin(1/110) / pCSII-EF
Alternative name FUCCI-Green
Clone info. Lentivirus vector construct of mAG-hGeminin(1/110). One of the component of Fucci cell cycle probe. Fluorescence was detected in the nucleus.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (Plasmid)
Size of vector backbone 9.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Human Geminin cDNA Galaxea fascicularis GFP cDNA
Originator Miyawaki, Atsushi |
 
Other clones in our bank human GMNN (NCBI Gene 51053) |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Ordering instruction. 
Order form 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Additional form)) Dr. Miyawaki's Approval
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15268 mAG-hGeminin(1/110) / pCSII-EF DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Fucci-related materials (en) (English text)
Featured content Fucci-related materials (ja) (Japanese text)

References

original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15268_A7H3p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15268_A7H3a.seq
>D04498H8_2_A7H3_EF1a-C_F_B01_04_ABI24.ab1
    1 TCATGTGGAT TTGCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG
   61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAACACG CTACCGGTCT CGAGAATTCG
  121 CCACCATGGT GAGCGTGATC AAGCCCGAGA TGAAGATCAA GCTGTGCATG AGGGGCACCG
  181 TGAACGGCCA CAACTTCGTG ATCGAGGGCG AGGGCAAGGG CAACCCCTAC GAGGGCACCC
  241 AGATCCTGGA CCTGAACGTG ACCGAGGGCG CCCCCCTGCC CTTCGCCTAC GACATCCTGA
  301 CCACCGTGTT CCAGTACGGC AACAGGGCCT TCACCAAGTA CCCCGCCGAC ATCCAGGACT
  361 ACTTCAAGCA GACCTTCCCC GAGGGCTACC ACTGGGAGAG GAGCATGACC TACGAGGACC
  421 AGGGCATCTG CACCGCCACC AGCAACATCA GCATGAGGGG CGACTGCTTC TTCTACGACA
  481 TCAGGTTCGA CGGCACCAAC TTCCCCCCCA ACGGCCCCGT GATGCAGAAG AAGACCCTGA
  541 AGTGGGAGCC CAGCACCGAG AAGATGTACG TGGAGGACGG CGTGCTGAAG GGCGACGTGA
  601 ACATGAGGCT GCTGCTGGAG GGCGGCGGCC ACTACAGGTG CGACTTCAAG ACCACCTACA
  661 AGGCCAAGAA GGAGGTGAGG CTGCCCGACG CCCACAAGAT CGACCACAGG ATCGAGATCC
  721 TGAAGCACGA CAAGGACTAC AACAAGGTGA AGCTGTACGA GAACGCCGTG GCCAGGTACT
  781 CCATGCTGCC CAGCCAGGCC AAGGGATATC CATCACACTG GCGGCCGCTC GAGATGAATC
  841 CCAGTATGAA GCAGAAACAA GAAGAAATCA AAGAGAATAT AAAGAATAGT TCTGTCCCAA
  901 GAAGAACTCT GAAGATGATT CAGCCTTCTG CATCTGGATC TCTTGTTGGA AGAGAAAATG
  961 AGCTGTCCGC AGCTTGTCCA AAAGGAACAT CGGGAATGAC CACTTAACAT CTACAACTTC
 1021 CAGCCCTGGG GGTTATTGTC CAGAATCTAG TGAAAATAAA AATCTTGAGA GTCACCCAGG
 1081 AGTTCATTGA TCCTATGATT AAAGAAAAAT CCAATCGTCT CAGTAATGGA GGAAGTGCAG
1141 GAAAACGGGA GAAAAGGCCG CCTGTTAATC A
//
Primer: BGH_rev RDB15268_A7H3b.seq
>D04498H8-2_A7H3_PRE_rev_B02_05_ABI24.ab1
    1 ACGAATCCAA ATAAGCGTAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA
   61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
  121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT
  181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT
  241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC
  301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG
  361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT
  421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC
  481 CCTTGGCCTG GCTGGGCAGC ATGGAGTACC TGGCCACGGC GTTCTCGTAC AGCTTCACCT
  541 TGTTGTAGTC CTTGTCGTGC TTCAGGATCT CGATCCTGTG GTCGATCTTG TGGGCGTCGG
  601 GCAGCCTCAC CTCCTTCTTG GCCTTGTAGG TGGTCTTGAA GTCGCACCTG TAGTGGCCGC
  661 CGCCCTCCAG CAGCAGCCTC ATGTTCACGT CGCCCTTCAG CACGCCGTCC TCCACGTACA
  721 TCTTCTCGGT GCTGGGCTCC CACTTCAGGG TCTTCTTCTG CATCACGGGG CCGTTGGGGG
  781 GGAAGTTGGT GCCGTCGAAC CTGATGTCGT AGAAGAAGCA GTCGCCCCTC ATGCTGATGT
  841 TGCTGGTGGC GGTGCAGATG CCCTGGTCCT CGTAGGTCAT GCTCCTCTCC CAGTGGTAGC
  901 CCTCGGGGAA GGTCTGCTTG AAGTAGTCCT GGATGTCGGC GGGGTACTTG GTGAAGGCCC
  961 TGTTGCCGTA CTGGAACACG GTGGTCAGGA TGTCGTAGGC GAAGGACAAG GGGGGCGCCC
 1021 TCGGTCCACG TTCAAGGTCC AGGATCTGGG TGCCCTCGTA AGGGGTTGCC CTTGCCCTTC
 1081 GCCTCGAATC ACGAAGTTGT GGCGATCACG GGTGGCCCCC TCATGCCACA GACTGAATCT
 1141 CATCTTCGGG CTGGATCCAG CGCTCACGAT GGGTGGCGAA TTCTCTGCAA GGAAACCGGC
1201 GTATGACCG
//
Primer: CMV-Forward RDB15268_A7H3c.seq
>D04498H8-2_A7H3_CMV-Forward_A09_03_ABI24.ab1
    1 TTGTTACAAC TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
  121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
  181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
  241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG
  301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC
  361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA
  421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT
  481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG
  541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA
  601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT
  661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA
  721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG
  781 CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA
841 ATATAAAGTA GTA
//
Primer: BGH_rev2 RDB15268_A7H3d.seq
>D04498H8-2_A7H3_BGH_rev2_B04_04_ABI24.ab1
    1 CTTGCAGCGG TCAGTAAGCA CGGGGGAGGG GCAAGCAACA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
  121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
  181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
  241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
  301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
  361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
  421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
  481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
  541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
  601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
  661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
  721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
  781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGAGGGCGAA
  841 GGCGAAGACG CGGAAGAGGC CGCAGAGCCG GCAGCAGGCC GCGGGAAAGG AAGGTCCGCT
  901 GGATTGAGGG CCGAAAGGGA CGTAGCAGAA GGACGTCCCG CGCAGAATCC AGGTGGGCAA
  961 CACAGGCGAG CAGCCATGGG AAAAGGACGT CAGCTTTCCC CGACAACACC ACGGGAATTG
 1021 TCAGTGCCCA CAGCCGAGCC CCTGTCCAGC AGCGGGGCAG CAGCGCGATG AGGTCCGTCG
 1081 TGGCATAGGA GGGAAGCGAA AGTCTGGGGA GCTGACAGTG GTGCCATGGC CCGACCAATG
1141 GGGGGTTGGC GC
//
Primer: SV40pro_F RDB15268_A7H3deseq
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078.

2018.10.09

GNP_filter3_RDBDEP_html_180823.pl