Sequencing and PCR primers for primer information.
Lentivirus vector construct of mAG-hGeminin(1/110). Component of Fucci cell cycle probe.
| Catalog number | RDB15268 |
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| Resource name | mAG-hGeminin(1/110) / pCSII-EF |
| Alternative name | FUCCI-Green |
| Clone info. | Lentivirus vector construct of mAG-hGeminin(1/110). One of the component of Fucci cell cycle probe. Fluorescence was detected in the nucleus. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
| Vector backbone | CSII-EF-MCS (Plasmid) |
| Size of vector backbone | 9.1 kb |
| Selectable markers | Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells). |
| Gene/insert name | Human Geminin cDNA Galaxea fascicularis GFP cDNA |
| Originator | Miyawaki, Atsushi | |
| Terms and conditions for distribution | 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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| Ordering | Please visit Ordering instruction. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
| Remarks | Remember that you will be working with samples containing infectious virus. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15268 | mAG-hGeminin(1/110) / pCSII-EF | DNA solution |
Electronic file
Featured content
| Featured content | Fucci-related materials (en) (English text) |
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| Featured content | Fucci-related materials (ja) (Japanese text) |
References
| original | Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15268_A7H3p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: EF1a-C_F | RDB15268_A7H3a.seq |
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>D04498H8_2_A7H3_EF1a-C_F_B01_04_ABI24.ab1 1 TCATGTGGAT TTGCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG 61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAACACG CTACCGGTCT CGAGAATTCG 121 CCACCATGGT GAGCGTGATC AAGCCCGAGA TGAAGATCAA GCTGTGCATG AGGGGCACCG 181 TGAACGGCCA CAACTTCGTG ATCGAGGGCG AGGGCAAGGG CAACCCCTAC GAGGGCACCC 241 AGATCCTGGA CCTGAACGTG ACCGAGGGCG CCCCCCTGCC CTTCGCCTAC GACATCCTGA 301 CCACCGTGTT CCAGTACGGC AACAGGGCCT TCACCAAGTA CCCCGCCGAC ATCCAGGACT 361 ACTTCAAGCA GACCTTCCCC GAGGGCTACC ACTGGGAGAG GAGCATGACC TACGAGGACC 421 AGGGCATCTG CACCGCCACC AGCAACATCA GCATGAGGGG CGACTGCTTC TTCTACGACA 481 TCAGGTTCGA CGGCACCAAC TTCCCCCCCA ACGGCCCCGT GATGCAGAAG AAGACCCTGA 541 AGTGGGAGCC CAGCACCGAG AAGATGTACG TGGAGGACGG CGTGCTGAAG GGCGACGTGA 601 ACATGAGGCT GCTGCTGGAG GGCGGCGGCC ACTACAGGTG CGACTTCAAG ACCACCTACA 661 AGGCCAAGAA GGAGGTGAGG CTGCCCGACG CCCACAAGAT CGACCACAGG ATCGAGATCC 721 TGAAGCACGA CAAGGACTAC AACAAGGTGA AGCTGTACGA GAACGCCGTG GCCAGGTACT 781 CCATGCTGCC CAGCCAGGCC AAGGGATATC CATCACACTG GCGGCCGCTC GAGATGAATC 841 CCAGTATGAA GCAGAAACAA GAAGAAATCA AAGAGAATAT AAAGAATAGT TCTGTCCCAA 901 GAAGAACTCT GAAGATGATT CAGCCTTCTG CATCTGGATC TCTTGTTGGA AGAGAAAATG 961 AGCTGTCCGC AGCTTGTCCA AAAGGAACAT CGGGAATGAC CACTTAACAT CTACAACTTC 1021 CAGCCCTGGG GGTTATTGTC CAGAATCTAG TGAAAATAAA AATCTTGAGA GTCACCCAGG 1081 AGTTCATTGA TCCTATGATT AAAGAAAAAT CCAATCGTCT CAGTAATGGA GGAAGTGCAG 1141 GAAAACGGGA GAAAAGGCCG CCTGTTAATC A // |
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| Primer: BGH_rev | RDB15268_A7H3b.seq |
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>D04498H8-2_A7H3_PRE_rev_B02_05_ABI24.ab1 1 ACGAATCCAA ATAAGCGTAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA 61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT 121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT 181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT 241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC 301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG 361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT 421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC 481 CCTTGGCCTG GCTGGGCAGC ATGGAGTACC TGGCCACGGC GTTCTCGTAC AGCTTCACCT 541 TGTTGTAGTC CTTGTCGTGC TTCAGGATCT CGATCCTGTG GTCGATCTTG TGGGCGTCGG 601 GCAGCCTCAC CTCCTTCTTG GCCTTGTAGG TGGTCTTGAA GTCGCACCTG TAGTGGCCGC 661 CGCCCTCCAG CAGCAGCCTC ATGTTCACGT CGCCCTTCAG CACGCCGTCC TCCACGTACA 721 TCTTCTCGGT GCTGGGCTCC CACTTCAGGG TCTTCTTCTG CATCACGGGG CCGTTGGGGG 781 GGAAGTTGGT GCCGTCGAAC CTGATGTCGT AGAAGAAGCA GTCGCCCCTC ATGCTGATGT 841 TGCTGGTGGC GGTGCAGATG CCCTGGTCCT CGTAGGTCAT GCTCCTCTCC CAGTGGTAGC 901 CCTCGGGGAA GGTCTGCTTG AAGTAGTCCT GGATGTCGGC GGGGTACTTG GTGAAGGCCC 961 TGTTGCCGTA CTGGAACACG GTGGTCAGGA TGTCGTAGGC GAAGGACAAG GGGGGCGCCC 1021 TCGGTCCACG TTCAAGGTCC AGGATCTGGG TGCCCTCGTA AGGGGTTGCC CTTGCCCTTC 1081 GCCTCGAATC ACGAAGTTGT GGCGATCACG GGTGGCCCCC TCATGCCACA GACTGAATCT 1141 CATCTTCGGG CTGGATCCAG CGCTCACGAT GGGTGGCGAA TTCTCTGCAA GGAAACCGGC 1201 GTATGACCG // |
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| Primer: CMV-Forward | RDB15268_A7H3c.seq |
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>D04498H8-2_A7H3_CMV-Forward_A09_03_ABI24.ab1 1 TTGTTACAAC TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT 61 ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA 121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT 181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT 241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG 301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC 361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA 421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT 481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG 541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA 601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT 661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA 721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG 781 CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA 841 ATATAAAGTA GTA // |
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| Primer: BGH_rev2 | RDB15268_A7H3d.seq |
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>D04498H8-2_A7H3_BGH_rev2_B04_04_ABI24.ab1 1 CTTGCAGCGG TCAGTAAGCA CGGGGGAGGG GCAAGCAACA GATGGCTGGC AACTAGAAGG 61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT 121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT 181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA 241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC 301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG 361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG 421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG 481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT 541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC 601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA 661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG 721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT 781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGAGGGCGAA 841 GGCGAAGACG CGGAAGAGGC CGCAGAGCCG GCAGCAGGCC GCGGGAAAGG AAGGTCCGCT 901 GGATTGAGGG CCGAAAGGGA CGTAGCAGAA GGACGTCCCG CGCAGAATCC AGGTGGGCAA 961 CACAGGCGAG CAGCCATGGG AAAAGGACGT CAGCTTTCCC CGACAACACC ACGGGAATTG 1021 TCAGTGCCCA CAGCCGAGCC CCTGTCCAGC AGCGGGGCAG CAGCGCGATG AGGTCCGTCG 1081 TGGCATAGGA GGGAAGCGAA AGTCTGGGGA GCTGACAGTG GTGCCATGGC CCGACCAATG 1141 GGGGGTTGGC GC // |
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| Primer: SV40pro_F | RDB15268_A7H3deseq |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078. |
|---|
2019.01.08
GNP_filter3_RDBDEP_html_181012.pl