RDB15268 - mAG-hGeminin(1/110) / pCSII-EF

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

mAG-hGeminin(1/110) / pCSII-EF (#RDB15268)

Lentivirus vector plasmid of mAG-hGeminin(1/110). Component of Fucci cell cycle probe.

Alternative name	FUCCI-Green
Clone info.	Lentivirus vector plasmid of mAG-hGeminin(1/110). One of the component of Fucci cell cycle probe. Fluorescence was detected in the nucleus.
Vector backbone	CSII-EF-MCS (RDB04378) (Plasmid)
Size of vector backbone	9.1 kb
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name	Human Geminin cDNA Galaxea fascicularis GFP cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. MTAに書く付加的使用条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考	【追加提供依頼書】受入れ確認書(書式J) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15268	mAG-hGeminin(1/110) / pCSII-EF	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mAG-hGeminin(1/110) / pCSII-EF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15268).

Reference section:

Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Osawa, H., Kashiwagi, S., Fukami, K., Miyata, T., Miyoshi, H., Imamura, T., Ogawa, M., Masai, H., Miyawaki, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15268_A7H3p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(mAG 5') Sequence file: RDB15268_A7H3g.seq
>D04498H8_2_A7H3_EF1a-C_F_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNTTTT GAGTTTGGNN CTTGGTTNNT TCTCNAGCCT CAGACAGTGG 61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAACACG CTACCGGTCT CGAGAATTCG 121 CCACCATGGT GAGCGTGATC AAGCCCGAGA TGAAGATCAA GCTGTGCATG AGGGGCACCG 181 TGAACGGCCA CAACTTCGTG ATCGAGGGCG AGGGCAAGGG CAACCCCTAC GAGGGCACCC 241 AGATCCTGGA CCTGAACGTG ACCGAGGGCG CCCCCCTGCC CTTCGCCTAC GACATCCTGA 301 CCACCGTGTT CCAGTACGGC AACAGGGCCT TCACCAAGTA CCCCGCCGAC ATCCAGGACT 361 ACTTCAAGCA GACCTTCCCC GAGGGCTACC ACTGGGAGAG GAGCATGACC TACGAGGACC 421 AGGGCATCTG CACCGCCACC AGCAACATCA GCATGAGGGG CGACTGCTTC TTCTACGACA 481 TCAGGTTCGA CGGCACCAAC TTCCCCCCCA ACGGCCCCGT GATGCAGAAG AAGACCCTGA 541 AGTGGGAGCC CAGCACCGAG AAGATGTACG TGGAGGACGG CGTGCTGAAG GGCGACGTGA 601 ACATGAGGCT GCTGCTGGAG GGCGGCGGCC ACTACAGGTG CGACTTCNAG ACCACCTACA 661 AGGCCAAGAA GGAGGTGAGG CTGCCCGACG CCCACAAGAT CGACCACAGG ATCGAGATCC 721 TGAAGCACGA CAAGGACTAC AACAAGGTGA AGCTGTACGA GAACGCCGTG NCCAGGTACT 781 CCNTGCTGCC CAGCCAGGCN NAGGGATATC CNTCACACNG GCGGCCGCTC GAGATGAATC 841 CCAGTATGAA GCAGAAACAA NAAGAAATCA AAGANAATAT AAAGAATAGT TCTGNCCNNN 901 NAAGAACTNN GNNNANGANT CAGCNNTCNG NATCNGNNTN TCTNNNNNNN NNANAAAATG 961 ANCTGNNNNN NGNNNGNNNN AANNNNNNNT CNGNANNGAN NNNNTNNNNN NNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,insert 3'(hGeminin(1/110),mAG 3') Sequence file: RDB15268_A7H3h.seq
>D04498H8-2_A7H3_PRE_rev_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNNN NNNAGCNNNN TAGTTAAGAA TACCNGTCAA TCTTTCACAA 61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT 121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT 181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT 241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC 301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG 361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT 421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC 481 CCTTGGCCTG GCTGGGCAGC ATGGAGTACC TGGCCACGGC GTTCTCGTAC AGCTTCACCT 541 TGTTGTAGTC CTTGTCGTGC TTCAGGATCT CGATCCTGTG GTCGATCTTG TGGGCGTCGG 601 GCAGCCTCAC CTCCTTCTTG GCCTTGTAGG TGGTCTTGAA GTCGCACCTG TAGTGGCCGC 661 CGCCCTCCAG CAGCAGCCTC ATGTTCACGT CGCCCTTCAG CACGCCGTCC TCCACGTACA 721 TCTTCTCGGT GCTGGGCTCC CACTTCAGGG TCTTCTTCTG CATCACGGGG CCGTTGGGGG 781 GGAAGTTGGT GCCGTCNAAN CTGATGTCGT ANAAGAANCA NTCNCCCCTC ATGCTGNNGT 841 TGCTGGTGGC GGTGCAGATG CCCTGGNCCT CGNANGTCAT GCTCCTCTCC NNGTNGTAGC 901 CCTCNGGGAA NGNCTGCTTG NANNNNNNCT GNNNGNCNGC GGGGTACTTG GTGAANGNCC 961 NGNTGCNNTN NTNNAACNNN GTGNNCNNGN NGTCNNNNNN NNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1 psi Sequence file: RDB15268_A7H3i.seq
>D04498H8-2_A7H3_CMV-Forward_A09_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNCNT NGNNGCNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT 61 ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA 121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT 181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT 241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG 301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC 361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA 421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT 481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG 541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA 601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT 661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA 721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCANGGGCCG 781 CTGATCTTCA GACCTGNAGG ANGAGATATG AGGGACNATT GGAGAAGTGA ATTATATAAA 841 TATNAAGNNN TAAAAATTGA ACCNTNNNGN NNNNCACCNN NNNNNGNNAN NNNNNGANNG 901 NNNNNNNNNN //
Primer: cPPT/CTS-E_R (Pr0725) Region: cPPT_CTS,RRE Sequence file: RDB15268_A7H3j.seq
>15268_15268_B0B4F_cPPT_CTS-E_R_F02_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCT TTAGTTTGTA TGTCTGTTGC TATTATGTCT 61 ACTATTCTTT CCCCTGCACT GTACCCCCCA ATCCCCCCTT TTCTTTTAAA ATTGTGGATG 121 AATACTGCCA TTTGTAGATC CGTTCACTAA TCGAATGGAT CTGTCTCTGT CTCTCTCTCC 181 ACCTTCTTCT TCTATTCCTT CGGGCCTGTC GGGTCCCCTC GGGGTTGGGA GGTGGGTCTG 241 AAACGATAAT GGTGAATATC CCTGCCTAAC TCTATTCACT ATAGAAAGTA CAGCAAAAAC 301 TATTCTTAAA CCTACCAAGC CTCCTACTAT CATTATGAAT AATTTTATAT ACCACAGCCA 361 ATTTGTTATG TTAAACCAAT TCCACAAACT TGCCCATTTA TCTAATTCCA ATAATTCTTG 421 TTCATTCTTT TCTTGCTGGT TTTGCGATTC TTCAATTAAG GAGTGTATTA AGCTTGTGTA 481 ATTGTTAATT TCTCTGTCCC ACTCCATCCA GGTCGTGTGA TTCCAAATCT GTTCCAGAGA 541 TTTATTACTC CAACTAGCAT TCCAAGGCAC AGCAGTGGTG CAAATGAGTT TTCCAGAGCA 601 ACCCCAAATC CCCAGGAGCT GTTGATCCTT TAGGTATCTT TCCACAGCCA GGATTCTTGC 661 CTGGAGCTGC TTGATGCCCC AGACTGTGAG TTGCAACAGA TGCTGTTGCG CCTCAATAGC 721 CCTCAGCAAA TTGTTCTGCT GCTGCACTAT ANCAGACAAT AATTGTCTGG CCTGTACCGT 781 CNGCGTCATT GANGCTGCGC CCATAGTGCT TCCTGCTGCT CCCNAGAACN CAAGGANCAA 841 AGCTNCTATT NCNACNGCTC TTTTTTTCTC TCTGCNNNAC TCNNNTCTTN GCCTTNNNGG 901 GNGCNACNNC TNANGGTNCN NTTTTTACTA CTTTATATTT ATATNANNCA CTTCTCNNAN 961 NNNNCCNNNA NATNNNCNNN NNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA,3' LTR Sequence file: RDB15268_A7H3k.seq
>D04498H8-2_A7H3_BGH_rev2_B04_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGNGGNNGG GNNNNNNNCA GATGGCTGGC ANCTAGAAGG 61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT 121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT 181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA 241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC 301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG 361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG 421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG 481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT 541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC 601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA 661 AAGGTACCTG AGGTGTGACT GGAAAANCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG 721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCNTGT TTTTCTAGGT 781 CTCGACNGTA TCGATGCGGG GANGCGGNCC AAAGGGNGAT CCGACTCGTC TGAGGGCGAA 841 NGCGAAGANN CGGAAGANGN NGCANANCNN GCAGCANNNC NCGGGNANGG AAGGNCCNCT 901 NGNTTGAGGG NCNAANNGNN NNNNNCANAN NNACNNNCCN CGCANAATCC NNGNNGNNNN 961 CNCNNNNNAN CNGCNNNNGN NAANNNNNNN CNNCNTNNNN NNNNNN //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori,Zeo,SV40 pA Sequence file: RDB15268_A7H3l.seq
>D04498H8-2_A7H3_SV40pro_F_B05_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGTGAN GNNGNTTTTT TGGAGGCCTA GGCTTTTGCA 61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA 121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC 181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT 241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT 301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC 361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT 421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG 481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA 541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACN AATAAAGCAA 721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCN ACCTCTAGCT AGAGCTNGNN 841 GNAATCATGG NCATAGCTGN TNNCTGTGTG AAATTGNTAT CCGCNCNCNA TTCCACACNN 901 CATACGANCC NGANNCATAA NGTGNAAAGN CNGGGGNGNC NNNNGAGTGA NCTNNCTNNN 961 ATTNNTTGCN NNNNNCNNNN NGNCNNNNTT CCNNNNNGNA ANNNNGNCNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078. [link to RRC of NBRP]
reference	Singer, D., Prominin-1 controls stem cell activation by orchestrating ciliary dynamics. EMBO J. 38 (2). pii: e99845 (2019). PMID 30523147.
reference	Tan, Z., Mammalian Cells Undergo Endoreduplication in Response to Lactic Acidosis. Sci Rep. 8 (1): 2890 (2018). PMID 29440645.
reference	Takahashi, H., Significance of Polypyrimidine Tract-Binding Protein 1 Expression in Colorectal Cancer. Mol. Cancer. Ther. 14 (7): 1705-1716 (2015). PMID 25904505. [link to RRC of NBRP]
reference	Kagawa, Y., Cell Cycle-Dependent Rho GTPase Activity Dynamically Regulates Cancer Cell Motility and Invasion In Vivo. PLoS One, 8 (12): e83629 (2013). PMID 24386239. [link to RRC of NBRP]
user_report	Onozato, Y., Radiosensitivity of quiescent and proliferating cells grown as multicellular tumor spheroids. Cancer Sci. 108 (4): 704-712 (2017). PMID 28135769. [link to RRC of NBRP]
user_report	Sereti, K.I., Methods to study the proliferation and differentiation of cardiac side population (CSP) cells. Methods Mol. Biol. 1036: 95-106 (2013). PMID 23807790. [link to RRC of NBRP]

Resource search

Link

Other resources in our bank

human Geminin

External Database
Human Geminin

Galaxea fascicularis GFP

Reference sequence