Please review the QC test results indicated by icon below as well as clone information before placing your order.

mAG-hGeminin(1/110) / pCSII-EF

Lentivirus vector plasmid of mAG-hGeminin(1/110). Component of Fucci cell cycle probe.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	【追加提供依頼書】受入れ確認書(書式J) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15268_A7H3p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Sequence file: RDB15268_A7H3a.seq
>D04498H8_2_A7H3_EF1a-C_F_B01_04_ABI24.ab1     1 TCATGTGGAT TTGCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG    61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAACACG CTACCGGTCT CGAGAATTCG   121 CCACCATGGT GAGCGTGATC AAGCCCGAGA TGAAGATCAA GCTGTGCATG AGGGGCACCG   181 TGAACGGCCA CAACTTCGTG ATCGAGGGCG AGGGCAAGGG CAACCCCTAC GAGGGCACCC   241 AGATCCTGGA CCTGAACGTG ACCGAGGGCG CCCCCCTGCC CTTCGCCTAC GACATCCTGA   301 CCACCGTGTT CCAGTACGGC AACAGGGCCT TCACCAAGTA CCCCGCCGAC ATCCAGGACT   361 ACTTCAAGCA GACCTTCCCC GAGGGCTACC ACTGGGAGAG GAGCATGACC TACGAGGACC   421 AGGGCATCTG CACCGCCACC AGCAACATCA GCATGAGGGG CGACTGCTTC TTCTACGACA   481 TCAGGTTCGA CGGCACCAAC TTCCCCCCCA ACGGCCCCGT GATGCAGAAG AAGACCCTGA   541 AGTGGGAGCC CAGCACCGAG AAGATGTACG TGGAGGACGG CGTGCTGAAG GGCGACGTGA   601 ACATGAGGCT GCTGCTGGAG GGCGGCGGCC ACTACAGGTG CGACTTCAAG ACCACCTACA   661 AGGCCAAGAA GGAGGTGAGG CTGCCCGACG CCCACAAGAT CGACCACAGG ATCGAGATCC   721 TGAAGCACGA CAAGGACTAC AACAAGGTGA AGCTGTACGA GAACGCCGTG GCCAGGTACT   781 CCATGCTGCC CAGCCAGGCC AAGGGATATC CATCACACTG GCGGCCGCTC GAGATGAATC   841 CCAGTATGAA GCAGAAACAA GAAGAAATCA AAGAGAATAT AAAGAATAGT TCTGTCCCAA   901 GAAGAACTCT GAAGATGATT CAGCCTTCTG CATCTGGATC TCTTGTTGGA AGAGAAAATG   961 AGCTGTCCGC AGCTTGTCCA AAAGGAACAT CGGGAATGAC CACTTAACAT CTACAACTTC  1021 CAGCCCTGGG GGTTATTGTC CAGAATCTAG TGAAAATAAA AATCTTGAGA GTCACCCAGG  1081 AGTTCATTGA TCCTATGATT AAAGAAAAAT CCAATCGTCT CAGTAATGGA GGAAGTGCAG 1141 GAAAACGGGA GAAAAGGCCG CCTGTTAATC A //
Primer: PRE_rev Sequence file: RDB15268_A7H3f.seq
>D04498H8-2_A7H3_PRE_rev_B02_05_ABI24.ab1     1 ACGAATCCAA ATAAGCGTAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA    61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT   121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT   181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT   241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC   301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG   361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT   421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC   481 CCTTGGCCTG GCTGGGCAGC ATGGAGTACC TGGCCACGGC GTTCTCGTAC AGCTTCACCT   541 TGTTGTAGTC CTTGTCGTGC TTCAGGATCT CGATCCTGTG GTCGATCTTG TGGGCGTCGG   601 GCAGCCTCAC CTCCTTCTTG GCCTTGTAGG TGGTCTTGAA GTCGCACCTG TAGTGGCCGC   661 CGCCCTCCAG CAGCAGCCTC ATGTTCACGT CGCCCTTCAG CACGCCGTCC TCCACGTACA   721 TCTTCTCGGT GCTGGGCTCC CACTTCAGGG TCTTCTTCTG CATCACGGGG CCGTTGGGGG   781 GGAAGTTGGT GCCGTCGAAC CTGATGTCGT AGAAGAAGCA GTCGCCCCTC ATGCTGATGT   841 TGCTGGTGGC GGTGCAGATG CCCTGGTCCT CGTAGGTCAT GCTCCTCTCC CAGTGGTAGC   901 CCTCGGGGAA GGTCTGCTTG AAGTAGTCCT GGATGTCGGC GGGGTACTTG GTGAAGGCCC   961 TGTTGCCGTA CTGGAACACG GTGGTCAGGA TGTCGTAGGC GAAGGACAAG GGGGGCGCCC  1021 TCGGTCCACG TTCAAGGTCC AGGATCTGGG TGCCCTCGTA AGGGGTTGCC CTTGCCCTTC  1081 GCCTCGAATC ACGAAGTTGT GGCGATCACG GGTGGCCCCC TCATGCCACA GACTGAATCT  1141 CATCTTCGGG CTGGATCCAG CGCTCACGAT GGGTGGCGAA TTCTCTGCAA GGAAACCGGC 1201 GTATGACCG //
Primer: CMV_Forward Sequence file: RDB15268_A7H3c.seq
>D04498H8-2_A7H3_CMV-Forward_A09_03_ABI24.ab1     1 TTGTTACAAC TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT    61 ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA   121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT   181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT   241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG   301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC   361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA   421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT   481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG   541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA   601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT   661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA   721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG   781 CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA 841 ATATAAAGTA GTA //
Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(mAG 5') Sequence file: RDB15268_A7H3g.seq
>D04498H8_2_A7H3_EF1a-C_F_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTTT GAGTTTGGNN CTTGGTTNNT TCTCNAGCCT CAGACAGTGG    61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAACACG CTACCGGTCT CGAGAATTCG   121 CCACCATGGT GAGCGTGATC AAGCCCGAGA TGAAGATCAA GCTGTGCATG AGGGGCACCG   181 TGAACGGCCA CAACTTCGTG ATCGAGGGCG AGGGCAAGGG CAACCCCTAC GAGGGCACCC   241 AGATCCTGGA CCTGAACGTG ACCGAGGGCG CCCCCCTGCC CTTCGCCTAC GACATCCTGA   301 CCACCGTGTT CCAGTACGGC AACAGGGCCT TCACCAAGTA CCCCGCCGAC ATCCAGGACT   361 ACTTCAAGCA GACCTTCCCC GAGGGCTACC ACTGGGAGAG GAGCATGACC TACGAGGACC   421 AGGGCATCTG CACCGCCACC AGCAACATCA GCATGAGGGG CGACTGCTTC TTCTACGACA   481 TCAGGTTCGA CGGCACCAAC TTCCCCCCCA ACGGCCCCGT GATGCAGAAG AAGACCCTGA   541 AGTGGGAGCC CAGCACCGAG AAGATGTACG TGGAGGACGG CGTGCTGAAG GGCGACGTGA   601 ACATGAGGCT GCTGCTGGAG GGCGGCGGCC ACTACAGGTG CGACTTCNAG ACCACCTACA   661 AGGCCAAGAA GGAGGTGAGG CTGCCCGACG CCCACAAGAT CGACCACAGG ATCGAGATCC   721 TGAAGCACGA CAAGGACTAC AACAAGGTGA AGCTGTACGA GAACGCCGTG NCCAGGTACT   781 CCNTGCTGCC CAGCCAGGCN NAGGGATATC CNTCACACNG GCGGCCGCTC GAGATGAATC   841 CCAGTATGAA GCAGAAACAA NAAGAAATCA AAGANAATAT AAAGAATAGT TCTGNCCNNN   901 NAAGAACTNN GNNNANGANT CAGCNNTCNG NATCNGNNTN TCTNNNNNNN NNANAAAATG 961 ANCTGNNNNN NGNNNGNNNN AANNNNNNNT CNGNANNGAN NNNNTNNNNN NNNNN //
Primer: BGH_rev2 Sequence file: RDB15268_A7H3d.seq
>D04498H8-2_A7H3_BGH_rev2_B04_04_ABI24.ab1     1 CTTGCAGCGG TCAGTAAGCA CGGGGGAGGG GCAAGCAACA GATGGCTGGC AACTAGAAGG    61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT   121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT   181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA   241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC   301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG   361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG   421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG   481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT   541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC   601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA   661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG   721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT   781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGAGGGCGAA   841 GGCGAAGACG CGGAAGAGGC CGCAGAGCCG GCAGCAGGCC GCGGGAAAGG AAGGTCCGCT   901 GGATTGAGGG CCGAAAGGGA CGTAGCAGAA GGACGTCCCG CGCAGAATCC AGGTGGGCAA   961 CACAGGCGAG CAGCCATGGG AAAAGGACGT CAGCTTTCCC CGACAACACC ACGGGAATTG  1021 TCAGTGCCCA CAGCCGAGCC CCTGTCCAGC AGCGGGGCAG CAGCGCGATG AGGTCCGTCG  1081 TGGCATAGGA GGGAAGCGAA AGTCTGGGGA GCTGACAGTG GTGCCATGGC CCGACCAATG 1141 GGGGGTTGGC GC //
Primer: SV40pro_F Sequence file: RDB15268_A7H3e.seq
>D04498H8-2_A7H3_SV40pro_F_B05_05_ABI24.ab1     1 CCCCGGTCAC GGGACTTTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA    61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA   121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC   181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT   241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT   301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC   361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT   421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG   481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA   541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG   601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT   661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA   721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC   781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC   841 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA   901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC   961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA  1021 TTTAATGATC GGCCAACGCG CGGGGAGAGG CGTTTTGCGT ATTGGGGCGC TTCTTCGGCT  1081 TCTCGCTCAC TGACTCGCTG CGCTCGGTCG TCCGGCCTGC GGCGAGCCGT ATCAGCTTCA 1141 CTCAGCGTTA GTACGGCTTA TCTCCCACC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl