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mVenus-zGeminin (1/100) / pT2KXIGdeltain

zGem-based S/G2/M marker for zebrafish cells to label nuclei with yellow fluorescent protein mVenus.

Catalog number RDB15279
Resource name mVenus-zGeminin (1/100) / pT2KXIGdeltain
Clone info. zGem-based S/G2/M marker for zebrafish cells to label nuclei with yellow fluorescent protein mVenus.
Refer Fukuhara, S. et al., Dev. Biol. 393 (1): 10-23, 2014.
Vector backbone pT2KXIGdeltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Gene/insert name zebrafish zGeminin cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AB971707 (DDBJ accession number)
Depositor Miyawaki, Atsushi |
 
Other clones in our bank zebrafish gmnn (KEGG orthology K10749) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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RDB15279 mVenus-zGeminin (1/100) / pT2KXIGdeltain DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Fukuhara, S., Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev. Biol. 393 (1): 10-23 (2014). PMID 24975012.
original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Transposon and plasmid, Kawakami laboratory (English text)
Featured content Fucci-related materials (en) (English text)
Featured content Fucci-related materials (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15279_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: beta-gi_F RDB15279_A7H9a.seq
>D04498J1_A7H9_2_beta-gi_F_H06_08_ABI08.ab1
    1 CAGGATTCAT GCCTTCTTCT CTTTCTACAG CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG
   61 TCTCATCATT TTGGCAAAGA ATTCCTCGAC GGATCCACTA GTAACGGCCG CCAGTGTGCT
  121 GGAATTCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
  181 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
  241 CACCTACGGC AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG
  301 GCCCACCCTC GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA
  361 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC
  421 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA
  481 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT
  541 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCACCG CCGACAAGCA
  601 GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCGGCGTGCA
  661 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA
  721 CAACCACTAC CTGAGCTACC AGTCCAAGCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA
  781 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA
  841 CAAGGGATAT CCATCACACT GGCGGCCGCT CGAGATGAGT TCCATCAGAA GACCAAAGAA
  901 TGCAGAAAAC CCGTCTGAAA ACATCAAGAA ATTCCTGGTA GCGCCAACAT CTGGAGCAGG
  961 AATGGGGAGG AGGACGCTTC AGTGCTTCAG CCCTCTGCTG TAAACAAAAA CCTTGGTCGT
 1021 ATAATTGAGA ATGGTAAGCA GTGGCCAAAA GGAAGATGTG AGCGCCGAGC AAGTTAAAGG
 1081 TTCAAAGAAG AGTCAAAGCA GGAGGTGCCT GTCCAATCCA CAATGCAGGA AATGGAAAAC
1141 AGCCCCTGGA AGGGGGGTCT CACCAACCCA
//
Primer: pAxCALNL_F1 RDB15279_A7H9b.seq
>D04498J1_A7H9_2_pAxCALNL_F1_F07_16_ABI24.ab1
    1 CTCCAGTGGT TTCTTAATCA ATGTCTGGAT CATCAATCGA TTACTCATAG GCCTCTTGTG
   61 TGACCCCCTC AGGCTGGTTT TCATTTTCTG CATTTGTGGA TTTGACAGCC ACCTCTGCTT
  121 TGACTCTCTT TGAACCCTTA ACTTGCTCGG CGCTCCACAT CTTCCTTTTG GCCACTGCTT
  181 TACCATTCTC AATTATACGA CCAAGGTTTT TGTTTACAGC AGAGGGCTGA AGCACCTGAA
  241 GCGTCCTCCT CCCCATTCCT GCTCCAGATG TTGGCGCTAC CAGGAATTTC TTGATGTTTT
  301 CAGACGGGTT TTCTGCATTC TTTGGTCTTC TGATGGAACT CATCTCGAGC GGCCGCCAGT
  361 GTGATGGATA TCCCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA
  421 ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGC TTGGACTGGT
  481 AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT
  541 GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTGG
  601 CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG TTGTAGTTGT
  661 ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA
  721 TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT
  781 CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA
  841 AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCACGCCG TAGCCCAGGG
  901 TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGGTGCAGAT CAGCTTCAGG
  961 GGTCAGCTTG CCGTAGGTGG CATCGCCCTC GCCCTCGGCC GGGACACGCC TGAACTTGGT
 1021 GGGCCGTTTA CCGTCCGCCG GTCCAGCTCG AACCAAGGAT GGGGCACCCA CCCCCCGGAT
 1081 GAAACAGGCT CTCGGCCCGT TGCTCCACCA ATGGATTCCA AGCACCAACT TGGCGGCCGT
1141 TAACCTATAG GTGGGAATCT CCCGAGTC
//
Primer: EF1a-N_R2 RDB15279_A7H9c.seq
>D04498J1_A7H9_2_EF1a-N_R2_G07_19_ABI24.ab1
    1 CTTAACGGGC CAAAGAATTG CAAGTTTGAT TTGCATTGGA AAGGGTCGCT GGCTTTTGTG
   61 TTACACGCCC CTTATTTGTG CTTGATTAGA TGATCCCCCT GCTCGAGGGC CCATCTGGCC
  121 TGTGTTTCAG ACACCAGGGA GTCTCTGCTC ACGTTTCCTG CTATTTGCAG CCTCTCTATC
  181 AAGACTAATA CACCTCTTCC CGCATCGGCT GCCTGTGAGA GGCTTTTCAG CACTGCAGGA
  241 TTGCTTTTCA GCCCCAAAAG AGCTAGGCTT GACACTAACA ATTTTGAGAA TCAGCTTCTA
  301 CTGAAGTTAA ATCTGAGGTT TTACAACTTT GAGTAGCGTG TACTGGCATT AGATTGTCTG
  361 TCTTATAGTT TGATAATTAA ATACAAACAG TTCTAAAGCA GGATAAAACC TTGTATGCAT
  421 TTCATTTAAT GTTTTTTGAG ATTAAAAGCT TAAACAAGAA TCTCTAGTTT TCTTTCTTGC
  481 TTTTACTTTT ACTTCCTTAA TACTCAAGTA CAATTTTAAT GGAGTACTTT TTTACTTTTA
  541 CTCAAGTAAG ATTCTAGCCA GATACTTTTA CTTTTAATTG AGTAAAATTT TCCCTAAGTA
  601 CTTGTACTTT CACTTGAGTA AAATTTTTGA GTACTTTTTA CACCTCTGTC AAGAACTCCT
  661 GGACAAACCT CTGACCTGTG TGGAACAGAG TGGATATGGG TGTCTGAACA GATATTCACG
  721 TCTTTTGCAG ATCAGAGGGC ATTTCTGGTG TTGTGCAATG ACCTGGGTCC AACAGGTAGG
  781 CGTACTCGTA TCCAAGAGCA TTGTTGGACA GGAGGTAGTC TCCATTCCTA TAAAGAGGAA
  841 GGAATGGCAC CATGTAGTAG CCGTCATTGT GGCCAATTGG TGCATTGGTA CGTGGGGTAG
  901 ATGGACCGGG GGAGGCTGAT GAGTTCTTAG CCCAGCGCTC AAAGATGCTA GAAATGATGT
  961 AAAGAAACAA ATATGCAGAT GAAAGTTTAA GATAAGATAA AAAATTTAAA CAAATAAATG
 1021 CAATTTATGG TTTTGTGTTT CTATTCCACC CAAATAATAA CCTCAACGGC AGTAAAGCCC
 1081 ACCAGGATAA TTTTTTGTCC ATGCTCTGCA AGGCATCCGA ACTCTTAGCA TGAATCTCTC
1141 CA
//
Primer: pAxEF_R2 RDB15279_A7H9d.seq
>D04498J1_A7H9_2_pAxEF_R2_H07_22_ABI24.ab1
    1 TGGGGAAAAA ATGCCTTTTT ATTTTGGTGA AATTTGTGGA TGCTATTGCT TTAATTTGTA
   61 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG
  121 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
  181 GCTGATTATG ATCCTCTAGA TCAGATCTGC GAAGATACGG CCACGGGTGC TCTTGATCCT
  241 GTGGCTGATT TTGGACTGTG CTGCTCGCAG CTGCTGATGA ATCACATACT TCCTCCATTT
  301 TCTTCCACTG ATTGACTGTT ATAATTTCCC TAATTTCCAG GTCAAGGTGC TGTGCATTGT
  361 GGTAATAGAT GTGACATGAC GTCACTTCCA AAGGACCAAT GAACATGTCT GACCAATTTC
  421 ATATAATGTG AAAACGATTT TCATAGGCAG AATAAATAAC ATTTAAATTA AACTGGGCAT
  481 CAGCGCAATT CAATTGGTTT GGTAATAGCA AGGGAAAATA GAATGAAGTG ATCTCCAAAA
  541 AATAAGTACT TTTTGACTGT AAATAAAATT GTAAGGAGTA AAAAGTACTT TTTTTTCTAA
  601 AAAAATGTAA TTAAGTAAAA GTAAAAGTAT TGATTTTTAA TTGTACTCAA GTAAAGTAAA
  661 AATCCCCAAA AATAATACTT AAGTACAGTA ATCAAGTAAA ATTACTCAAG TACTTTACAC
  721 CTCTGGTTCT TGACCCCCTA CCTTCAGCAA AGCCCAGCAG ATCCACTAGT TCTAGAGCGG
  781 CCGCCAACCG CGGTGGAGCT CCAGCTTTTG TTCCCTTTTA GTGAGGGTTA ATTGCGCGCT
  841 TGGCGTAATC ATGGTCATAA GCTGGTTTCC TGTGTGAAAT TGTTAATCCG CTCACAATTT
  901 CCACACAACA TACGAAGCCG GAAGGCATAA AGTGTAAGCC CTGGGTGCCT AAATGAGTGA
  961 GCTAAACTCA CATTAAATTG CGTTGCGCTC ACTGCCCGCT TTTCCAAGTC GGGAAAACCT
 1021 GTTCGTGGCC AAGCTGCATT AAATGAAATT CGGGCCAACG CGCGGGGGGA AGAAGGCGGT
 1081 TTGCGTAATG GGCGCTCCTT TCCGGCATTC CCTCGCTTCA CTTGACCTCC CCTGCCGCCC
1141 TCGGCTCGAT TCGGACCTGG CGGGGCGAAG ACGCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Fukuhara, S., Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev. Biol. 393 (1): 10-23 (2014). PMID 24975012.
original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430.
reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.

2019.08.08

GNP_filter3_RDBDEP_html_190432.pl