RDB15279 - mVenus-zGeminin (1/100) / pT2KXIGdeltain

zGem-based S/G2/M marker for zebrafish cells to label nuclei with yellow fluorescent protein mVenus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution	The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内)

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提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
付加的提供条件	Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	提供条件は英文のまま記載してください。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15279	mVenus-zGeminin (1/100) / pT2KXIGdeltain	DNA solution

original	Fukuhara, S., Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev. Biol. 393 (1): 10-23 (2014). PMID 24975012.
original	Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430.

Featured content	Transposon and plasmid, Kawakami laboratory (English text)
Featured content	Fucci-related materials (en) (English text)
Featured content	Fucci-related materials (ja) (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: beta-gi_F Region: insert 5' Sequence file: RDB15279_A7H9a.seq
>D04498J1_A7H9_2_beta-gi_F_H06_08_ABI08.ab1 1 CAGGATTCAT GCCTTCTTCT CTTTCTACAG CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG 61 TCTCATCATT TTGGCAAAGA ATTCCTCGAC GGATCCACTA GTAACGGCCG CCAGTGTGCT 121 GGAATTCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA 181 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC 241 CACCTACGGC AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG 301 GCCCACCCTC GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA 361 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC 421 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA 481 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT 541 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCACCG CCGACAAGCA 601 GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCGGCGTGCA 661 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA 721 CAACCACTAC CTGAGCTACC AGTCCAAGCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA 781 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA 841 CAAGGGATAT CCATCACACT GGCGGCCGCT CGAGATGAGT TCCATCAGAA GACCAAAGAA 901 TGCAGAAAAC CCGTCTGAAA ACATCAAGAA ATTCCTGGTA GCGCCAACAT CTGGAGCAGG 961 AATGGGGAGG AGGACGCTTC AGTGCTTCAG CCCTCTGCTG TAAACAAAAA CCTTGGTCGT 1021 ATAATTGAGA ATGGTAAGCA GTGGCCAAAA GGAAGATGTG AGCGCCGAGC AAGTTAAAGG 1081 TTCAAAGAAG AGTCAAAGCA GGAGGTGCCT GTCCAATCCA CAATGCAGGA AATGGAAAAC 1141 AGCCCCTGGA AGGGGGGTCT CACCAACCCA //
Primer: pAxCALNL_F1 Region: insert 3' Sequence file: RDB15279_A7H9b.seq
>D04498J1_A7H9_2_pAxCALNL_F1_F07_16_ABI24.ab1 1 CTCCAGTGGT TTCTTAATCA ATGTCTGGAT CATCAATCGA TTACTCATAG GCCTCTTGTG 61 TGACCCCCTC AGGCTGGTTT TCATTTTCTG CATTTGTGGA TTTGACAGCC ACCTCTGCTT 121 TGACTCTCTT TGAACCCTTA ACTTGCTCGG CGCTCCACAT CTTCCTTTTG GCCACTGCTT 181 TACCATTCTC AATTATACGA CCAAGGTTTT TGTTTACAGC AGAGGGCTGA AGCACCTGAA 241 GCGTCCTCCT CCCCATTCCT GCTCCAGATG TTGGCGCTAC CAGGAATTTC TTGATGTTTT 301 CAGACGGGTT TTCTGCATTC TTTGGTCTTC TGATGGAACT CATCTCGAGC GGCCGCCAGT 361 GTGATGGATA TCCCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA 421 ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGC TTGGACTGGT 481 AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT 541 GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTGG 601 CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG TTGTAGTTGT 661 ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA 721 TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT 781 CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA 841 AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCACGCCG TAGCCCAGGG 901 TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGGTGCAGAT CAGCTTCAGG 961 GGTCAGCTTG CCGTAGGTGG CATCGCCCTC GCCCTCGGCC GGGACACGCC TGAACTTGGT 1021 GGGCCGTTTA CCGTCCGCCG GTCCAGCTCG AACCAAGGAT GGGGCACCCA CCCCCCGGAT 1081 GAAACAGGCT CTCGGCCCGT TGCTCCACCA ATGGATTCCA AGCACCAACT TGGCGGCCGT 1141 TAACCTATAG GTGGGAATCT CCCGAGTC //
Primer: EF1a-N_R2 Region: Tol2 R Sequence file: RDB15279_A7H9c.seq
>D04498J1_A7H9_2_EF1a-N_R2_G07_19_ABI24.ab1 1 CTTAACGGGC CAAAGAATTG CAAGTTTGAT TTGCATTGGA AAGGGTCGCT GGCTTTTGTG 61 TTACACGCCC CTTATTTGTG CTTGATTAGA TGATCCCCCT GCTCGAGGGC CCATCTGGCC 121 TGTGTTTCAG ACACCAGGGA GTCTCTGCTC ACGTTTCCTG CTATTTGCAG CCTCTCTATC 181 AAGACTAATA CACCTCTTCC CGCATCGGCT GCCTGTGAGA GGCTTTTCAG CACTGCAGGA 241 TTGCTTTTCA GCCCCAAAAG AGCTAGGCTT GACACTAACA ATTTTGAGAA TCAGCTTCTA 301 CTGAAGTTAA ATCTGAGGTT TTACAACTTT GAGTAGCGTG TACTGGCATT AGATTGTCTG 361 TCTTATAGTT TGATAATTAA ATACAAACAG TTCTAAAGCA GGATAAAACC TTGTATGCAT 421 TTCATTTAAT GTTTTTTGAG ATTAAAAGCT TAAACAAGAA TCTCTAGTTT TCTTTCTTGC 481 TTTTACTTTT ACTTCCTTAA TACTCAAGTA CAATTTTAAT GGAGTACTTT TTTACTTTTA 541 CTCAAGTAAG ATTCTAGCCA GATACTTTTA CTTTTAATTG AGTAAAATTT TCCCTAAGTA 601 CTTGTACTTT CACTTGAGTA AAATTTTTGA GTACTTTTTA CACCTCTGTC AAGAACTCCT 661 GGACAAACCT CTGACCTGTG TGGAACAGAG TGGATATGGG TGTCTGAACA GATATTCACG 721 TCTTTTGCAG ATCAGAGGGC ATTTCTGGTG TTGTGCAATG ACCTGGGTCC AACAGGTAGG 781 CGTACTCGTA TCCAAGAGCA TTGTTGGACA GGAGGTAGTC TCCATTCCTA TAAAGAGGAA 841 GGAATGGCAC CATGTAGTAG CCGTCATTGT GGCCAATTGG TGCATTGGTA CGTGGGGTAG 901 ATGGACCGGG GGAGGCTGAT GAGTTCTTAG CCCAGCGCTC AAAGATGCTA GAAATGATGT 961 AAAGAAACAA ATATGCAGAT GAAAGTTTAA GATAAGATAA AAAATTTAAA CAAATAAATG 1021 CAATTTATGG TTTTGTGTTT CTATTCCACC CAAATAATAA CCTCAACGGC AGTAAAGCCC 1081 ACCAGGATAA TTTTTTGTCC ATGCTCTGCA AGGCATCCGA ACTCTTAGCA TGAATCTCTC 1141 CA //
Primer: pAxEF_R2 Region: Tol2 L Sequence file: RDB15279_A7H9d.seq
>D04498J1_A7H9_2_pAxEF_R2_H07_22_ABI24.ab1 1 TGGGGAAAAA ATGCCTTTTT ATTTTGGTGA AATTTGTGGA TGCTATTGCT TTAATTTGTA 61 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG 121 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG 181 GCTGATTATG ATCCTCTAGA TCAGATCTGC GAAGATACGG CCACGGGTGC TCTTGATCCT 241 GTGGCTGATT TTGGACTGTG CTGCTCGCAG CTGCTGATGA ATCACATACT TCCTCCATTT 301 TCTTCCACTG ATTGACTGTT ATAATTTCCC TAATTTCCAG GTCAAGGTGC TGTGCATTGT 361 GGTAATAGAT GTGACATGAC GTCACTTCCA AAGGACCAAT GAACATGTCT GACCAATTTC 421 ATATAATGTG AAAACGATTT TCATAGGCAG AATAAATAAC ATTTAAATTA AACTGGGCAT 481 CAGCGCAATT CAATTGGTTT GGTAATAGCA AGGGAAAATA GAATGAAGTG ATCTCCAAAA 541 AATAAGTACT TTTTGACTGT AAATAAAATT GTAAGGAGTA AAAAGTACTT TTTTTTCTAA 601 AAAAATGTAA TTAAGTAAAA GTAAAAGTAT TGATTTTTAA TTGTACTCAA GTAAAGTAAA 661 AATCCCCAAA AATAATACTT AAGTACAGTA ATCAAGTAAA ATTACTCAAG TACTTTACAC 721 CTCTGGTTCT TGACCCCCTA CCTTCAGCAA AGCCCAGCAG ATCCACTAGT TCTAGAGCGG 781 CCGCCAACCG CGGTGGAGCT CCAGCTTTTG TTCCCTTTTA GTGAGGGTTA ATTGCGCGCT 841 TGGCGTAATC ATGGTCATAA GCTGGTTTCC TGTGTGAAAT TGTTAATCCG CTCACAATTT 901 CCACACAACA TACGAAGCCG GAAGGCATAA AGTGTAAGCC CTGGGTGCCT AAATGAGTGA 961 GCTAAACTCA CATTAAATTG CGTTGCGCTC ACTGCCCGCT TTTCCAAGTC GGGAAAACCT 1021 GTTCGTGGCC AAGCTGCATT AAATGAAATT CGGGCCAACG CGCGGGGGGA AGAAGGCGGT 1081 TTGCGTAATG GGCGCTCCTT TCCGGCATTC CCTCGCTTCA CTTGACCTCC CCTGCCGCCC 1141 TCGGCTCGAT TCGGACCTGG CGGGGCGAAG ACGCG //