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pBS coupler 6

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Catalog number RDB15131
Resource name pBS coupler 6
Clone info. Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
Vector backbone Plasmid
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15131 pBS coupler 6 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15131_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: insert, f1 ori | Primer: M13R | Sequence: RDB15131_A7H9a.seq
>D04498B7_A7H9_1_M13R_E03_15_ABI24.ab1
    1 TGGCGGCAAT TTAAACCCCT CACTAAAAGG GGAACAAAAG CTGGGTACCG GGGCCCCCCC
   61 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT GGCGATCCGC CACCGCCAGA GCCACCTCCG
  121 CCTGAACCGC CTCCACCATC GAATTCCTGC AGCCCGGGGG ATCCACTAGT TCTAGAGCGG
  181 CCGCCACCGC GGTGGAGCTC CAATTCGCCC TATAGTGAGT CGTATTACGC GCGCTCACTG
  241 GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT
  301 GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT
  361 TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGAAATTGT AAGCGTTAAT ATTTTGTTAA
  421 AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA
  481 AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA
  541 ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC
  601 AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC
  661 GTAAAGCACT AAATCGGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG
  721 CCGGCGAACG TGGCGGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT
  781 GGGCAAGTGT AGCGGGTCAC GCTGCGCGTA ACCCACCAAC ACCCGGCCGC GGCTTAATGC
  841 GCCGCTACAA GGGCGCGGTC AGGTGGCACT TTTTCGGGGA AAATGTGCGC GGGAACCCCC
  901 CTATTTGGTT TATTTTTCTA AATAGATTTC AAATATGGTA TCCGCTCCAT GAGAACAATA
  961 ACCCTGGATA AATGCTTCAA TAATATTTGG AAAAAAGGAA AAGTATAGAG TAATTCCAAC
 1021 ATTTTCCGTG GTCGCCCCTC TAATGTCCCC TTTTTTGTGG CGGGCATTTA TGCCCTTCTC
 1081 TGTGTTTTGC GCTACACACC AGAAACGCTC GTGGTGAAAA AGATTAAAGA ATGCCTTGAA
1141 GAATGCGTGG GTTGCAACGG ACAGGTGGGT GTTTACCACT GTGACATACT GATTGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471.

2019.11.02

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