Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBS coupler 6 (#RDB15131)

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Clone info. Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S,, Miyawaki, A., Methods Cell Biol. 85C: 381-393 (2008).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S,, Miyawaki, A., Methods Cell Biol. 85C: 381-393 (2008).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15131 pBS coupler 6 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBS coupler 6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15131).

Reference section:

Shimozono, S., Miyawaki, A., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15131_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13R (Pr0160)
Region: insert, f1 ori
Sequence file: RDB15131_A7H9a.seq check
>D04498B7_A7H9_1_M13R_E03_15_ABI24.ab1
    1 TGGCGGCAAT TTAAACCCCT CACTAAAAGG GGAACAAAAG CTGGGTACCG GGGCCCCCCC
   61 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT GGCGATCCGC CACCGCCAGA GCCACCTCCG
  121 CCTGAACCGC CTCCACCATC GAATTCCTGC AGCCCGGGGG ATCCACTAGT TCTAGAGCGG
  181 CCGCCACCGC GGTGGAGCTC CAATTCGCCC TATAGTGAGT CGTATTACGC GCGCTCACTG
  241 GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT
  301 GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT
  361 TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGAAATTGT AAGCGTTAAT ATTTTGTTAA
  421 AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA
  481 AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA
  541 ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC
  601 AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC
  661 GTAAAGCACT AAATCGGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG
  721 CCGGCGAACG TGGCGGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT
  781 GGGCAAGTGT AGCGGGTCAC GCTGCGCGTA ACCCACCAAC ACCCGGCCGC GGCTTAATGC
  841 GCCGCTACAA GGGCGCGGTC AGGTGGCACT TTTTCGGGGA AAATGTGCGC GGGAACCCCC
  901 CTATTTGGTT TATTTTTCTA AATAGATTTC AAATATGGTA TCCGCTCCAT GAGAACAATA
  961 ACCCTGGATA AATGCTTCAA TAATATTTGG AAAAAAGGAA AAGTATAGAG TAATTCCAAC
 1021 ATTTTCCGTG GTCGCCCCTC TAATGTCCCC TTTTTTGTGG CGGGCATTTA TGCCCTTCTC
 1081 TGTGTTTTGC GCTACACACC AGAAACGCTC GTGGTGAAAA AGATTAAAGA ATGCCTTGAA
1141 GAATGCGTGG GTTGCAACGG ACAGGTGGGT GTTTACCACT GTGACATACT GATTGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP]