Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

AzaleaB5/pRSETb

New coral derived red-emitting fluorescent protein AzaleaB5.

Catalog number RDB18218
Resource name AzaleaB5/pRSETb
Clone info. New coral derived red-emitting fluorescent protein AzaleaB5.
The accession numbers in the DDBJ/EMBL/GenBank database is LC085679.
Vector backbone pRSETb (plasmid)
Size of vector backbone 3.6 kb
Selectable markers Ampicillin
Gene/insert name Montipora monasteriata RFP cDNA
Reference of nucleotide sequence LC085679 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Sakaue-Sawano, Asako | Ando, Ryoko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18218 AzaleaB5/pRSETb DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18218_B0Fbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: T7pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB18218_B0Fba.seq check
>D7168A1_B0Fb_3_lacIpro-B_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGANNAGCG CCCAATACGC ANACCGCCTC
   61 TNNNNNNNNN CGTTGGCCGA TTCATTAATG CAGGATCTCG ATCCCGCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCACA ACGGTTTCCC TCTAGAAATA ATTTTGTTTA ACTTTAAGAA
  181 GGAGATATAC ATATGCGGGG TTCTCATCAT CATCATCATC ATGGTATGGC TAGCATGACT
  241 GGTGGACAGC AAATGGGTCG GGATCTGTAC GACGATGACG ATAAGGATCC GATGGAGAAC
  301 GTCAGGAGAA AGACAGGCAT TCAGACGGAA ATGAAAACGA AGCTCCACAT GGATGGTATG
  361 GTGAATGGAC ACAGCTTTGA GATCAAAGGG GAGGGGAAGG GAAGCCCGTA CGAGGGGGTG
  421 CAGACAATGA AGCTGAAGGT TACTAAGGGC GCCCCACTTC CCTTCTCTAT AGACATTCTT
  481 TTGCCTCAGT GCATGTATGG CAGCAAACCT TTCATTAAAT ATCCTGAGAA CATCCCCGAT
  541 TATTTGAAAC TCTCATTTCC AGAGGGTATC ACTTGGGAGA GGACAATGAC ATTCGAGGAC
  601 GGCGCAGTAT GCGATGTATC TAATGATTCC AGACTGGTCG GCAATTGCTT TATCTACACT
  661 GTGAAGTTTC AGGGAGTCAA TTTTCCACTT GATGGNCCNN TTATGCNAAA GAAGANCCGN
  721 NGCTGGNAAC CCTNCACTGA AGTGCTGTAC GAATGTGATG GGTGGATGCG CGGGTNGGTG
  781 GANATAGCTC TGAANNTNNN AAACGGNGGN NATTATATGT GTANTTTTNN NANCNNNNAN
  841 AANTCNNNNA AAGGNCTNGA NGNGNNNCNT TNNCNTTTCN NNNNNNNTNA NCNCNNNNTG
901 NNCNGNNNNN NNNNN
//
Primer: T7ter_R1
Region: insert 3'
Sequence file: RDB18218_B0Fbb.seq check
>D07168A1_B0Fb_3_T7ter_R1_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNGCNNG TNNNTGCTNN GCGGNGGCAG CAGCCAACTC AGCTTCCTTT
   61 CGGGCTTTGT TAGCAGCCGG ATCAAGCTTC GAATTCTCAG GCCAGTTTAC TCAAGTGCGC
  121 GTGAGCGATT TCACCTTGTT CGAACTCCTC AAAGGTGGCT CCGTCGGTGT TATGACTGAG
  181 CAGGTCGAGT TTATGATCCA CGAAATGGTA AGGTGGCACC TCAAGACCTT TCTTGGATTT
  241 GTAGGTGGTC TTAAAATTAC ACATATAATG TCCACCGTTT TCCAGTTTCA GAGCTATGTC
  301 CACCAACCCG CGCATCCACC CATCACATTC GTACAGCACT TCAGTGGAGG GTTCCCAGCC
  361 TCGGGTCTTC TTTTGCATAA CGGGGCCATC AAGTGGAAAA TTGACTCCCT GAAACTTCAC
  421 AGTGTAGATA AAGCAATTGC CGACCAGTCT GGAATCATTA GATACATCGC ATACTGCGCC
  481 GTCCTCGAAT GTCATTGTCC TCTCCCAAGT GATACCCTCT GGAAATGAGA GTTTCAAATA
  541 ATCGGGGATG TTCTCAGGAT ATTTAATGAA AGGTTTGCTG CCATACATGC ACTGAGGCAA
  601 AAGAATGTCT ATAGAGAAGG GAAGTGGGGC GCCCTTAGTA ACCTTCAGCT TCATTGTCTG
  661 CACCCCCTCG TACGGGCTTC CCTTCCCCTC CCCTTTGATC TCAAAGCTGT GTCCATTCAC
  721 CATACCATCN NTGTGGAGCT TCGTTTTCAT TTCCGTCTGA ATGCCTGTCT TTCTCCTGAN
  781 GTTCTCCATC GGATCCTTAT CGTCATCGTC GTACAGATCC CGACCCATTT GCTGNCCACC
  841 AGTCATGCTA NCCATACCAT GATGATGATG ANGATGAGAN NCCCGCANAT GTATATCTNC
  901 NTCTTAAAGT TAAACAAAAT TATTTCTANA NGGNAANCNN TNNGGTCTCC CTATANNGNN
961 NNNNATTAAT TNCGCNGGNN NCGANATNCN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

2020.12.17

GNP_filter3_RDBDEP_html_201216.pl