RDB18218 - AzaleaB5/pRSETb

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内)

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提供条件	1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18218	AzaleaB5/pRSETb	DNA solution

original	Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

Featured content	Fluorescent protein resources (English text)
Featured content	Fluorescent protein resources (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: lacIpro-B_F Region: T7pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB18218_B0Fba.seq
>D7168A1_B0Fb_3_lacIpro-B_F_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGANNAGCG CCCAATACGC ANACCGCCTC 61 TNNNNNNNNN CGTTGGCCGA TTCATTAATG CAGGATCTCG ATCCCGCGAA ATTAATACGA 121 CTCACTATAG GGAGACCACA ACGGTTTCCC TCTAGAAATA ATTTTGTTTA ACTTTAAGAA 181 GGAGATATAC ATATGCGGGG TTCTCATCAT CATCATCATC ATGGTATGGC TAGCATGACT 241 GGTGGACAGC AAATGGGTCG GGATCTGTAC GACGATGACG ATAAGGATCC GATGGAGAAC 301 GTCAGGAGAA AGACAGGCAT TCAGACGGAA ATGAAAACGA AGCTCCACAT GGATGGTATG 361 GTGAATGGAC ACAGCTTTGA GATCAAAGGG GAGGGGAAGG GAAGCCCGTA CGAGGGGGTG 421 CAGACAATGA AGCTGAAGGT TACTAAGGGC GCCCCACTTC CCTTCTCTAT AGACATTCTT 481 TTGCCTCAGT GCATGTATGG CAGCAAACCT TTCATTAAAT ATCCTGAGAA CATCCCCGAT 541 TATTTGAAAC TCTCATTTCC AGAGGGTATC ACTTGGGAGA GGACAATGAC ATTCGAGGAC 601 GGCGCAGTAT GCGATGTATC TAATGATTCC AGACTGGTCG GCAATTGCTT TATCTACACT 661 GTGAAGTTTC AGGGAGTCAA TTTTCCACTT GATGGNCCNN TTATGCNAAA GAAGANCCGN 721 NGCTGGNAAC CCTNCACTGA AGTGCTGTAC GAATGTGATG GGTGGATGCG CGGGTNGGTG 781 GANATAGCTC TGAANNTNNN AAACGGNGGN NATTATATGT GTANTTTTNN NANCNNNNAN 841 AANTCNNNNA AAGGNCTNGA NGNGNNNCNT TNNCNTTTCN NNNNNNNTNA NCNCNNNNTG 901 NNCNGNNNNN NNNNN //
Primer: T7ter_R1 Region: insert 3' Sequence file: RDB18218_B0Fbb.seq
>D07168A1_B0Fb_3_T7ter_R1_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNGCNNG TNNNTGCTNN GCGGNGGCAG CAGCCAACTC AGCTTCCTTT 61 CGGGCTTTGT TAGCAGCCGG ATCAAGCTTC GAATTCTCAG GCCAGTTTAC TCAAGTGCGC 121 GTGAGCGATT TCACCTTGTT CGAACTCCTC AAAGGTGGCT CCGTCGGTGT TATGACTGAG 181 CAGGTCGAGT TTATGATCCA CGAAATGGTA AGGTGGCACC TCAAGACCTT TCTTGGATTT 241 GTAGGTGGTC TTAAAATTAC ACATATAATG TCCACCGTTT TCCAGTTTCA GAGCTATGTC 301 CACCAACCCG CGCATCCACC CATCACATTC GTACAGCACT TCAGTGGAGG GTTCCCAGCC 361 TCGGGTCTTC TTTTGCATAA CGGGGCCATC AAGTGGAAAA TTGACTCCCT GAAACTTCAC 421 AGTGTAGATA AAGCAATTGC CGACCAGTCT GGAATCATTA GATACATCGC ATACTGCGCC 481 GTCCTCGAAT GTCATTGTCC TCTCCCAAGT GATACCCTCT GGAAATGAGA GTTTCAAATA 541 ATCGGGGATG TTCTCAGGAT ATTTAATGAA AGGTTTGCTG CCATACATGC ACTGAGGCAA 601 AAGAATGTCT ATAGAGAAGG GAAGTGGGGC GCCCTTAGTA ACCTTCAGCT TCATTGTCTG 661 CACCCCCTCG TACGGGCTTC CCTTCCCCTC CCCTTTGATC TCAAAGCTGT GTCCATTCAC 721 CATACCATCN NTGTGGAGCT TCGTTTTCAT TTCCGTCTGA ATGCCTGTCT TTCTCCTGAN 781 GTTCTCCATC GGATCCTTAT CGTCATCGTC GTACAGATCC CGACCCATTT GCTGNCCACC 841 AGTCATGCTA NCCATACCAT GATGATGATG ANGATGAGAN NCCCGCANAT GTATATCTNC 901 NTCTTAAAGT TAAACAAAAT TATTTCTANA NGGNAANCNN TNNGGTCTCC CTATANNGNN 961 NNNNATTAAT TNCGCNGGNN NCGANATNCN NNNNNNNNN //

AzaleaB5/pRSETb

Distribution information

References and tips

Sequence information

References