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AzaleaB5/pRSETb (#RDB18218)

New coral derived red-emitting fluorescent protein AzaleaB5.

Clone info. New coral derived red-emitting fluorescent protein AzaleaB5.
Comment The accession numbers in the DDBJ/EMBL/GenBank database is LC085679.
Vector backbone pRSETb (plasmid)
Size of vector backbone 3.6 kb
Selectable markers Ampicillin
Growth conditions LB+Amp, 37oC
Gene/insert name Montipora monasteriata RFP cDNA
Reference of insert sequence LC085679 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Sawano, Asako | Ando, Ryoko |
 
Sequence (full) RDB18218hts01.seq checkAssembled from experimentally sequenced data.
Publication Ando, R., Cell Struct. Funct. 48 (2): 135-144 (2023). PMID 37394513. [link to RRC of NBRP]
Test sheet RDB18218_B0Gfp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Ando, R. et al., bioRxiv doi: 10.1101/2020.03.30.015156 (2020).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Ando, R. et al., bioRxiv doi: 10.1101/2020.03.30.015156 (2020).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18218 AzaleaB5/pRSETb DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The AzaleaB5/pRSETb was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18218).

Reference section:

Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A., Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. Cell Struct. Funct. 48 (2): 135-144 (2023). PMID 37394513. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ando, R., Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. Cell Struct. Funct. 48 (2): 135-144 (2023). PMID 37394513. [link to RRC of NBRP]