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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

AzaleaB5/pRSETb

New coral derived red-emitting fluorescent protein AzaleaB5.

Catalog number RDB18218
Resource name AzaleaB5/pRSETb
Clone info. New coral derived red-emitting fluorescent protein AzaleaB5.
Comment The accession numbers in the DDBJ/EMBL/GenBank database is LC085679.
Vector backbone pRSETb (plasmid)
Size of vector backbone 3.6 kb
Selectable markers Ampicillin
Gene/insert name Montipora monasteriata RFP cDNA
Reference of insert sequence LC085679 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Sakaue-Sawano, Asako | Ando, Ryoko |
Other clones in our bank

External Database
Montipora monasteriata RFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
            4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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            MTA, for use for not-for-profit academic purpose [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
            4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18218 AzaleaB5/pRSETb DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The AzaleaB5/pRSETb was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18218).

            Reference section:

            Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


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            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18218_B0Fbp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F (Pr0724)
            Region: T7pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
            Sequence file: RDB18218_B0Fba.seq check
            >D7168A1_B0Fb_3_lacIpro-B_F_A01_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGANNAGCG CCCAATACGC ANACCGCCTC
               61 TNNNNNNNNN CGTTGGCCGA TTCATTAATG CAGGATCTCG ATCCCGCGAA ATTAATACGA
              121 CTCACTATAG GGAGACCACA ACGGTTTCCC TCTAGAAATA ATTTTGTTTA ACTTTAAGAA
              181 GGAGATATAC ATATGCGGGG TTCTCATCAT CATCATCATC ATGGTATGGC TAGCATGACT
              241 GGTGGACAGC AAATGGGTCG GGATCTGTAC GACGATGACG ATAAGGATCC GATGGAGAAC
              301 GTCAGGAGAA AGACAGGCAT TCAGACGGAA ATGAAAACGA AGCTCCACAT GGATGGTATG
              361 GTGAATGGAC ACAGCTTTGA GATCAAAGGG GAGGGGAAGG GAAGCCCGTA CGAGGGGGTG
              421 CAGACAATGA AGCTGAAGGT TACTAAGGGC GCCCCACTTC CCTTCTCTAT AGACATTCTT
              481 TTGCCTCAGT GCATGTATGG CAGCAAACCT TTCATTAAAT ATCCTGAGAA CATCCCCGAT
              541 TATTTGAAAC TCTCATTTCC AGAGGGTATC ACTTGGGAGA GGACAATGAC ATTCGAGGAC
              601 GGCGCAGTAT GCGATGTATC TAATGATTCC AGACTGGTCG GCAATTGCTT TATCTACACT
              661 GTGAAGTTTC AGGGAGTCAA TTTTCCACTT GATGGNCCNN TTATGCNAAA GAAGANCCGN
              721 NGCTGGNAAC CCTNCACTGA AGTGCTGTAC GAATGTGATG GGTGGATGCG CGGGTNGGTG
              781 GANATAGCTC TGAANNTNNN AAACGGNGGN NATTATATGT GTANTTTTNN NANCNNNNAN
              841 AANTCNNNNA AAGGNCTNGA NGNGNNNCNT TNNCNTTTCN NNNNNNNTNA NCNCNNNNTG
            901 NNCNGNNNNN NNNNN
            //
            Primer: T7ter_R1 (Pr0798)
            Region: insert 3'
            Sequence file: RDB18218_B0Fbb.seq check
            >D07168A1_B0Fb_3_T7ter_R1_A02_02_ABI24.ab1
                1 NNNNNNNNNN NNNNNGCNNG TNNNTGCTNN GCGGNGGCAG CAGCCAACTC AGCTTCCTTT
               61 CGGGCTTTGT TAGCAGCCGG ATCAAGCTTC GAATTCTCAG GCCAGTTTAC TCAAGTGCGC
              121 GTGAGCGATT TCACCTTGTT CGAACTCCTC AAAGGTGGCT CCGTCGGTGT TATGACTGAG
              181 CAGGTCGAGT TTATGATCCA CGAAATGGTA AGGTGGCACC TCAAGACCTT TCTTGGATTT
              241 GTAGGTGGTC TTAAAATTAC ACATATAATG TCCACCGTTT TCCAGTTTCA GAGCTATGTC
              301 CACCAACCCG CGCATCCACC CATCACATTC GTACAGCACT TCAGTGGAGG GTTCCCAGCC
              361 TCGGGTCTTC TTTTGCATAA CGGGGCCATC AAGTGGAAAA TTGACTCCCT GAAACTTCAC
              421 AGTGTAGATA AAGCAATTGC CGACCAGTCT GGAATCATTA GATACATCGC ATACTGCGCC
              481 GTCCTCGAAT GTCATTGTCC TCTCCCAAGT GATACCCTCT GGAAATGAGA GTTTCAAATA
              541 ATCGGGGATG TTCTCAGGAT ATTTAATGAA AGGTTTGCTG CCATACATGC ACTGAGGCAA
              601 AAGAATGTCT ATAGAGAAGG GAAGTGGGGC GCCCTTAGTA ACCTTCAGCT TCATTGTCTG
              661 CACCCCCTCG TACGGGCTTC CCTTCCCCTC CCCTTTGATC TCAAAGCTGT GTCCATTCAC
              721 CATACCATCN NTGTGGAGCT TCGTTTTCAT TTCCGTCTGA ATGCCTGTCT TTCTCCTGAN
              781 GTTCTCCATC GGATCCTTAT CGTCATCGTC GTACAGATCC CGACCCATTT GCTGNCCACC
              841 AGTCATGCTA NCCATACCAT GATGATGATG ANGATGAGAN NCCCGCANAT GTATATCTNC
              901 NTCTTAAAGT TAAACAAAAT TATTTCTANA NGGNAANCNN TNNGGTCTCC CTATANNGNN
            961 NNNNATTAAT TNCGCNGGNN NCGANATNCN NNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156. [link to RRC of NBRP]

            2024.03.26

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