Vector for Mitochondria labeling with fluorescent protein StayGold
Drawn by SnapGene® software |
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Clone info. | A soluble fluorescent marker of the mitochondrial matrix. n1 is the appendage for fusion of a tandem repeat of the cytochrome c oxidase subunit VIII presequence to the N-terminus of StayGold. The gene for StayGold in this construct has been resynthesized with mammalian-preferred codons. See Hirano, M. et al., Nat. Biotechnol. 2022 doi: 10.1038/s41587-022-01278-2 for more information. |
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Comment | The accession numbers in the DDBJ/EMBL/GenBank databases are LC601652 for StayGold, LC593679 for h-StayGold and LC601653 for oxStayGold. |
Vector backbone | CSII-EF-MCS (RDB04378) (plasmid) |
Size of vector backbone | 9.1 kb |
Selectable markers | Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
Growth remarks | Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
Gene/insert name | Cytaeis uchidae GFP cDNA human COX8A cDNA |
Depositor|Developer | Miyawaki, Atsushi | Hirano, Masahiko | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al. Nat. Biotechnol. 40 (7): 1132-1142 (2022). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
Remarks | Remember that you will be working with samples containing infectious virus. Please obtain written approval of Dr. Atsushi Miyawaki. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al. Nat. Biotechnol. 40 (7): 1132-1142 (2022). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB19614 | mt-(n1)StayGold | DNA solution |
Materials & Methods section:
The mt-(n1)StayGold was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19614). |
Reference section:
Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., Inaura, S., Matsumura, Y., Hama, H., Okada, Y., Fujiwara, T., Morimoto, T., Katayama, K., Miyawaki, A., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [link to RRC of NBRP] |
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