Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

mt-(n1)StayGold

Vector for Mitochondria labeling with fluorescent protein StayGold

Catalog number RDB19614
Resource name mt-(n1)StayGold
Clone info. A soluble fluorescent marker of the mitochondrial matrix. n1 is the appendage for fusion of a tandem repeat of the cytochrome c oxidase subunit VIII presequence to the N-terminus of StayGold. The gene for StayGold in this construct has been resynthesized with mammalian-preferred codons. See Hirano, M. et al., Nat. Biotechnol. 2022 doi: 10.1038/s41587-022-01278-2 for more information.
Comment The accession numbers in the DDBJ/EMBL/GenBank databases are LC601652 for StayGold, LC593679 for h-StayGold and LC601653 for oxStayGold.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Cytaeis uchidae GFP cDNA human COX8A cDNA
Depositor|Developer Miyawaki, Atsushi | Hirano, Masahiko |
 
Sequence (full) RDB19614hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19614_B2Eap1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment]  [Bank Transfer Payment]
MTA, for use for not-for-profit academic purpose [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Hirano, M. et al. Nat. Biotechnol. 40 (7): 1132-1142 (2022).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks Remember that you will be working with samples containing infectious virus.
Please obtain written approval of Dr. Atsushi Miyawaki.
提供案内 (日本国内) [open/close]

提供依頼書
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
宮脇敦史博士による提供承諾書 [Word]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Hirano, M. et al. Nat. Biotechnol. 40 (7): 1132-1142 (2022).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
提供条件は英文のまま記載してください。
宮脇敦史博士の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19614 mt-(n1)StayGold DNA solution


How to cite this biological resource

Materials & Methods section:

The mt-(n1)StayGold was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19614).

Reference section:

Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., Inaura, S., Matsumura, Y., Hama, H., Okada, Y., Fujiwara, T., Morimoto, T., Katayama, K., Miyawaki, A., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [link to RRC of NBRP]