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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mt-(n1)StayGold

Vector for Mitochondria labeling with fluorescent protein StayGold

Catalog number RDB19614
Resource name mt-(n1)StayGold
Clone info. A soluble fluorescent marker of the mitochondrial matrix. n1 is the appendage for fusion of a tandem repeat of the cytochrome c oxidase subunit VIII presequence to the N-terminus of StayGold. The gene for StayGold in this construct has been resynthesized with mammalian-preferred codons. See Hirano, M. et al., Nat. Biotechnol. 2022 doi: 10.1038/s41587-022-01278-2 for more information.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. The accession numbers in the DDBJ/EMBL/GenBank databases are LC601652 for StayGold, LC593679 for h-StayGold and LC601653 for oxStayGold.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Growth remarks Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Cytaeis uchidae GFP cDNA human COX8A cDNA
Depositor|Developer Miyawaki, Atsushi | Hirano, Masahiko |
Other clones in our bank

External Database
Cytaeis uchidae GFP
    human COX8A

          Reference sequence
            
           
          Sequence (full) RDB19614zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks Remember that you will be working with samples containing infectious virus.
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          宮脇敦史博士による提供承諾書 [Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19614 mt-(n1)StayGold DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content 明るく、退色しにくい新規の緑色蛍光タンパク質 「StayGold」 (Japanese text)
          Featured content StayGold, highly photostable GFP (English text)
          Featured content Visualization of Organelles (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19614_B2Eap1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Region: EF-1alpha pro,insert 5'(signal seq,(n1)StayGold 5')
          Sequence file: RDB19614_B2Eaa.seq check
          >D08836B1_B2Ea_1_EF1a-C_F_D11_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNCNNTN NNGAGTTTGG NNCTTGGTTN NTTCTCNAGC CTCAGACAGT
             61 GGTTCAAAGT TNNNNNTCNN NNNTTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGATG
            121 TCCGTCCTGA CGCCGCTGCT GCTGCGGGGC TTGACAGGCT CGGCCCGGCG GCTCCCAGTG
            181 CCGCGCGCCA AGATCCATTC GTTGCCGCCG GAGGGGAAGC TTGGGATGTC CGTCCTGACG
            241 CCGCTGCTGC TGCGGGGCTT GACAGGCTCG GCCCGGCGGC TCCCAGTGCC GCGCGCCAAG
            301 ATCCATTCGT TGCCGCCGGA GGGGAAGCTC GGGGGATCCA TGGCCAGCAC CGGGGAGGAG
            361 CTGTTCACCG GGGTGGTGCC CTTCAAGTTC CAGCTGAAGG GCACCATCAA CGGCAAGAGC
            421 TTCACCGTGG AAGGCGAGGG CGAGGGCAAT AGCCACGAGG GCAGCCACAA AGGCAAGTAC
            481 GTGTGCACCA GCGGCAAACT GCCAATGTCT TGGGCCGCCC TGGGAACTAG CTTCGGCTAT
            541 GGCATGAAGT ACTACACCAA GTACCCCAGC GGCCTGAAGA ACTGGTTCCA CGAGGTGATG
            601 CCCGAGGGCT TCACCTACGA CAGACACATC CAGTACAAGG GCGACGGCAG CATCCACGCC
            661 AAGCACCAGC ACTTCATGAA GAACGGCACC TACCACAACA TCGTGGAGTT CACCGGCCAN
            721 GACTTCNAGG AGAACAGCCC CGTGCTGACC GGCGACATGA ACGTGAGNCT GCCNNACGAG
            781 GTGCAGCACA TNCCCNGANA TGANNGCGNN NNNNGNCCAN NGNNCCTGCT GNANCCTCTG
            841 CNNANCGACN AGAGCNAGTG CGNNNNNGNC CNNNANAACN NNNTNNNNNN NCCNNNNNNN
          901 NATCANCNNN NCCNNNNNGN NNNNTACNNN NGNNTNNNAA NNNNNNNNNN
          //
          Primer: PRE_rev
          Region: WPRE,insert 3'((n1)StayGold 3')
          Sequence file: RDB19614_B2Eab.seq check
          >D08836B1_B2Ea_1_PRE_rev_E11_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNCNN NNNNNGNNNN NCNTAGTTAA GAATACCAGT CAATCTTTCN
             61 CNAATTTTGT AATCCNNANN NNGNTTATCG ATAAGCTTGA TCCCTCGATG TTAACTCTAG
            121 ATTACAGGTG GGCCTCCAGG GTCTCGCTCT GGCAGATGTG GTCTCTCTCC TCGGTGTCGT
            181 CCTTGCTCTG GGTGTACTGC TTTCTGATCC AGTGGTATGG CACATCGGGG GCTGGCTGAT
            241 TGTGCAGGGG CTTGCAGATG GTGTTCTGGT GGGCCTCCAC GCACTTGCTC TTGTCGCTCA
            301 GCAGAGGGTA CAGCAGGGTC ACTGGGCACT CCACGCCGTC ATCTCTGGGG ATGTGCTGCA
            361 CCTCGTTGGG CAGGCTCACG TTCATGTCGC CGGTCAGCAC GGGGCTGTTC TCCTTGAAGT
            421 CCTGGCCGGT GAACTCCACG ATGTTGTGGT AGGTGCCGTT CTTCATGAAG TGCTGGTGCT
            481 TGGCGTGGAT GCTGCCGTCG CCCTTGTACT GGATGTGTCT GTCGTAGGTG AAGCCCTCGG
            541 GCATCACCTC GTGGAACCAG TTCTTCAGGC CGCTGGGGTA CTTGGTGTAG TACTTCATGC
            601 CATAGCCGAA GCTAGTTCCC AGGGCGGCCC AAGACATTGG CAGTTTGCCG CTGGTGCACA
            661 CGTACTTGCC TTTGTGGCTG CCCTCGTGGC TATTGCCCTC GCCCTCGCCT TCCACGGTGA
            721 AGCTCTTGCC GTTGATGGTG CCCTTCAGCT GGAACTTGAA GGGCACCACC CCGGTGAACA
            781 GCTCCTCCCC GGNGCTGGCC ATGGATCCCC CGAGCTTCCC CTCCGGCGGC AACGAATGGA
            841 TCTTGGCGCG CGGNACTGGG GAGCCGCCNG GGCCGAGNNT GTCAAGNCCC GCANCANCAG
            901 CGGCGTCNNG NNGGANATCC NAAGCTTCCC CTCCNGCGGC NNCNANTGGA TCTTGGCGCG
            961 CGGCACTGGG NGNCNNCCNG GNCNANCCTG TCANGNCCNN CNNCNNNCNN CNNNNNNNNN
          1021 GNNNGANNNN NNNNNN
          //
          Primer: CMV_Forward
          Region: CMV pro,5' LTR,HIV-1 psi
          Sequence file: RDB19614_B2Eac.seq check
          >D08836B1_B2Ea_1-CMV_Forward_C06_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGNNNNNNGN NNNNNNTNNN
             61 NNNNCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG
            121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT
            181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT
            241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA
            301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG
            361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG
            421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC
            481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG
            541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA
            601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA
            661 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG
            721 CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGGGCCGC
            781 TGATCTTCAG ACCTGGANGA NGANATATGA NGGACAATTG GAGAAGTGAA TTATATAAAT
            841 ATAAAGTANT AAAAATTGAA CCATTANGAN TAGCACCCAC CNANGCAAAG AGAAGANTGG
            901 TGCANANAGA AAAAAGANCA NNNGGAATAN GANCTTTGTT CCNTGGGNTC NNGGGNNCAG
          961 CNNNAGCANT ANNGGCGNAG CNNNNNTNNN NNNGNNNNNA CNNNNNNNNN N
          //
          Primer: bGH_rev3
          Region: 5' LTR
          Sequence file: RDB19614_B2Ead.seq check
          >D08836B1_B2Ea_1-bGH_rev3_D06_12_ABI24.ab1
              1 NNNNNNNNNN NNNNGGNNGN NGGNNNNNNA CAGATGGCTG GCAACTAGAA GGCACAGTCG
             61 AGGCTGNNNN NCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA CTAAAAGGGT
            121 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT
            181 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG
            241 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC TCGGGCCATG
            301 TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC CGGGTCATCC
            361 ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT GGCTTCTTCT
            421 ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG TGGATATCTG
            481 ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT GTGGTAGATC
            541 CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG TCCCCCCTTT
            601 TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT TAAAGGTACC
            661 TGANNTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA GGCACAATCA
            721 GCATTGGTAG CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG GTCTCGACNG
            781 TATCGATGCG GGGNNGCGGC CCAAAGGGAN NTCCGACTCG TCTGANGGCG AANGCGAAGA
            841 CGCGGAANAN GCCGCAGANC CNGCAGCNNN CGCGGGAANG AANGNCCNCT GGNNTGAGGG
            901 NCNAANNGNA NGTANCANNN NNNGTCCCGC GNANAATCCN NGTGGNNACN CNNNNANCAG
          961 CCNNNGNAAA NGNANGTCNN CNTNNCCNGN NNNNNNNNN
          //
          Primer: RRE_F1
          Region: RRE,cPPT_CTS
          Sequence file: RDB19614_B2Eae.seq check
          >D08836B1_B2Ea_1-RRE_F1_E06_15_ABI24.ab1
              1 NNNNNNNNNN NNGNNNNNNN NNNNNNNNNN NNCNNANNNT TTGCTGNNGG CNATTGAGGC
             61 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT
            121 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA
            181 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA
            241 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT
            301 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT
            361 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA
            421 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT
            481 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT
            541 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA
            601 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTACAAAT GGCAGTATTC ATCCACAATT
            661 TTAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA GACATAATAG
            721 CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA AATTTTCGGG
            781 TTTATTACAG GGACAGCAGA NATTCACTTT GAATTAATTC AAGCTTCGTG AGGCTCCNGT
            841 GCCCGTCAGT GGGCAGANCG CACATCGCCC ACAGNCCCCG ANAANTTGGG GGGGANGGGT
            901 CGGCNATTGA ACCGGTGCCT ANAGAANNNG GNGCGGGGTA AACNGGNNAA GTGANGNCNN
            961 GNANTGGNTN CNCCNTTTTC CNNANGGNGG GGGANANNNN NNNATNNNNG CANTANTCNN
          1021 NNNNNNNN
          //
          Primer: EF1a-N_R
          Region: EF-1 alpha pro,cPPT_CTS
          Sequence file: RDB19614_B2Eaf.seq check
          >D08836B1_B2Ea_1-EF1a-N_R_F06_18_ABI24.ab1
              1 NNNNNNNNNN NGNNNNNNNG GCNNNGTGCG CTCTGCCNNC TGACGGGCAC CGGNNCCTCA
             61 CGAAGCTTGA ATTAATTCAA AGTGAATTTC TGCTGTCCCT GTAATAAACC CGAAAATTTT
            121 GAATTTTTGT AATTTGTTTT TGTAATTCTT TAGTTTGTAT GTCTGTTGCT ATTATGTCTA
            181 CTATTCTTTC CCCTGCACTG TACCCCCCAA TCCCCCCTTT TCTTTTAAAA TTGTGGATGA
            241 ATACTGCCAT TTGTAGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC TCTCTCTCCA
            301 CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG GTGGGTCTGA
            361 AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC AGCAAAAACT
            421 ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA CCACAGCCAA
            481 TTTGTTATGT TAAACCAATT CCACAAACTT GCCCATTTAT CTAATTCCAA TAATTCTTGT
            541 TCATTCTTTT CTTGCTGGTT TTGCGATTCT TCAATTAAGG AGTGTATTAA GCTTGTGTAA
            601 TTGTTAATTT CTCTGTCCCA CTCCATCCAG GTCGTGTGAT TCCAAATCTG TTCCAGAGAT
            661 TTATTACTCC AACTAGCATT CCAAGGCACA GCAGTGGTGC AAATGAGTTT TCCAGANCAA
            721 CCCCAAATCC CCAGGAGCTG TTGATCCTTT ANGTATCTTT CCACAGCCAG GANTCTTGCC
            781 TGGANCTGCT TGATGCCCCA GANTGTGAGT TGCAACAGAT GCTGTTGCGC CTCAATAGCC
            841 NTCAGCAAAT TGTTCTGCTG CTGCACTATA CCAGACAATA ATTGTCTGGN CNGTACCGTC
            901 AGCGTCATTG ANGCTGCGCC CATANTGCTN NCNGCTGCNN CCNAGANCCC NANGNAANAA
            961 AGCTCCNATT CCCNCNNNTC NTTTTNNNNN NNGCNNNNNT NNNNNNNTTN NCNNNNNNNG
          1021 NNNNNNNNNN
          //
          Primer: SV40_F2
          Region: WPRE,5' LTR
          Sequence file: RDB19614_B2Eag.seq check
          >D08836B1_B2Ea_1-SV40_F2_G06_21_ABI24.ab1
              1 NNNNNNNNNN NNNCNNNNNN TTNNGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC
             61 CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGAGACCTA GAAAAACATG GAGCAATCAC
            121 AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA
            181 GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC
            241 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA
            301 ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTG
            361 GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA
            421 GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA ACACCCGCTT
            481 GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT TAGAGTGGAG
            541 GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG ACTGTACTGG
            601 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT
            661 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG
            721 TGACTCTGGT AACTAGAGAT CCCTCAGANC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG
            781 GGCCCGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG
            841 TTTGCCCCTC CCCCGTGCCT TCCTTGANCC TGGAANGTGC CACTCCCACN NNCCTTNNCT
            901 AATAAAATGA NGAAANTGCA TCGNATTNNC TGANTANGNG TCATTCNATT CTNGGGGGGT
            961 GGGGTGGGGC NNNNAGCNNN GGGNNNNNNN NGNNNGNNNN TANCNNNNTG CNNGGGNNNN
          1021 NNNNGNNNNN NNNGNNNNNN NNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,EM7 pro,Zeo
          Sequence file: RDB19614_B2Eah.seq check
          >D08836B1_B2Ea_1-SV40pro_ori_F_H06_24_ABI24.ab1
              1 NNNNNNNNNN NNNNNGCCGC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT
             61 TTTGGANGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
            121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA
            181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG
            241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG
            301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG
            361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT
            421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA
            481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT
            541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG
            601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC
            661 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT
            721 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
            781 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT
            841 CNANCTCTAN CTAGANCTTG GCGTAATCAT GGNCATANCT GTTNCCNGTG TGAAATTGNT
            901 ATCCGCTCNC AATTCCNCAC AACATACNAG CNNGAAGCAT AAAGTGNAAA GCCNGGGNNG
            961 NCNAANGANT GANCTAACTC NNATTNNNNG CGTTGNNNNN NNNGNCCNNN TTNCANNNNN
          1021 NNAANNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl