Please review the QC test results indicated by icon below as well as clone information before placing your order.

mt-(n1)StayGold

Vector for Mitochondria labeling with fluorescent protein StayGold

Sequence (full)	RDB19614zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 宮脇敦史博士による提供承諾書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19614_B2Eap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1alpha pro,insert 5'(signal seq,(n1)StayGold 5') Sequence file: RDB19614_B2Eaa.seq
>D08836B1_B2Ea_1_EF1a-C_F_D11_04_ABI08.ab1     1 NNNNNNNNNN NNNNNCNNTN NNGAGTTTGG NNCTTGGTTN NTTCTCNAGC CTCAGACAGT    61 GGTTCAAAGT TNNNNNTCNN NNNTTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGATG   121 TCCGTCCTGA CGCCGCTGCT GCTGCGGGGC TTGACAGGCT CGGCCCGGCG GCTCCCAGTG   181 CCGCGCGCCA AGATCCATTC GTTGCCGCCG GAGGGGAAGC TTGGGATGTC CGTCCTGACG   241 CCGCTGCTGC TGCGGGGCTT GACAGGCTCG GCCCGGCGGC TCCCAGTGCC GCGCGCCAAG   301 ATCCATTCGT TGCCGCCGGA GGGGAAGCTC GGGGGATCCA TGGCCAGCAC CGGGGAGGAG   361 CTGTTCACCG GGGTGGTGCC CTTCAAGTTC CAGCTGAAGG GCACCATCAA CGGCAAGAGC   421 TTCACCGTGG AAGGCGAGGG CGAGGGCAAT AGCCACGAGG GCAGCCACAA AGGCAAGTAC   481 GTGTGCACCA GCGGCAAACT GCCAATGTCT TGGGCCGCCC TGGGAACTAG CTTCGGCTAT   541 GGCATGAAGT ACTACACCAA GTACCCCAGC GGCCTGAAGA ACTGGTTCCA CGAGGTGATG   601 CCCGAGGGCT TCACCTACGA CAGACACATC CAGTACAAGG GCGACGGCAG CATCCACGCC   661 AAGCACCAGC ACTTCATGAA GAACGGCACC TACCACAACA TCGTGGAGTT CACCGGCCAN   721 GACTTCNAGG AGAACAGCCC CGTGCTGACC GGCGACATGA ACGTGAGNCT GCCNNACGAG   781 GTGCAGCACA TNCCCNGANA TGANNGCGNN NNNNGNCCAN NGNNCCTGCT GNANCCTCTG   841 CNNANCGACN AGAGCNAGTG CGNNNNNGNC CNNNANAACN NNNTNNNNNN NCCNNNNNNN 901 NATCANCNNN NCCNNNNNGN NNNNTACNNN NGNNTNNNAA NNNNNNNNNN //
Primer: PRE_rev (Pr0250) Region: WPRE,insert 3'((n1)StayGold 3') Sequence file: RDB19614_B2Eab.seq
>D08836B1_B2Ea_1_PRE_rev_E11_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNCNN NNNNNGNNNN NCNTAGTTAA GAATACCAGT CAATCTTTCN    61 CNAATTTTGT AATCCNNANN NNGNTTATCG ATAAGCTTGA TCCCTCGATG TTAACTCTAG   121 ATTACAGGTG GGCCTCCAGG GTCTCGCTCT GGCAGATGTG GTCTCTCTCC TCGGTGTCGT   181 CCTTGCTCTG GGTGTACTGC TTTCTGATCC AGTGGTATGG CACATCGGGG GCTGGCTGAT   241 TGTGCAGGGG CTTGCAGATG GTGTTCTGGT GGGCCTCCAC GCACTTGCTC TTGTCGCTCA   301 GCAGAGGGTA CAGCAGGGTC ACTGGGCACT CCACGCCGTC ATCTCTGGGG ATGTGCTGCA   361 CCTCGTTGGG CAGGCTCACG TTCATGTCGC CGGTCAGCAC GGGGCTGTTC TCCTTGAAGT   421 CCTGGCCGGT GAACTCCACG ATGTTGTGGT AGGTGCCGTT CTTCATGAAG TGCTGGTGCT   481 TGGCGTGGAT GCTGCCGTCG CCCTTGTACT GGATGTGTCT GTCGTAGGTG AAGCCCTCGG   541 GCATCACCTC GTGGAACCAG TTCTTCAGGC CGCTGGGGTA CTTGGTGTAG TACTTCATGC   601 CATAGCCGAA GCTAGTTCCC AGGGCGGCCC AAGACATTGG CAGTTTGCCG CTGGTGCACA   661 CGTACTTGCC TTTGTGGCTG CCCTCGTGGC TATTGCCCTC GCCCTCGCCT TCCACGGTGA   721 AGCTCTTGCC GTTGATGGTG CCCTTCAGCT GGAACTTGAA GGGCACCACC CCGGTGAACA   781 GCTCCTCCCC GGNGCTGGCC ATGGATCCCC CGAGCTTCCC CTCCGGCGGC AACGAATGGA   841 TCTTGGCGCG CGGNACTGGG GAGCCGCCNG GGCCGAGNNT GTCAAGNCCC GCANCANCAG   901 CGGCGTCNNG NNGGANATCC NAAGCTTCCC CTCCNGCGGC NNCNANTGGA TCTTGGCGCG   961 CGGCACTGGG NGNCNNCCNG GNCNANCCTG TCANGNCCNN CNNCNNNCNN CNNNNNNNNN 1021 GNNNGANNNN NNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1 psi Sequence file: RDB19614_B2Eac.seq
>D08836B1_B2Ea_1-CMV_Forward_C06_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGNNNNNNGN NNNNNNTNNN    61 NNNNCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG   121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT   181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT   241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA   301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG   361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG   421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC   481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG   541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA   601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA   661 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG   721 CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGGGCCGC   781 TGATCTTCAG ACCTGGANGA NGANATATGA NGGACAATTG GAGAAGTGAA TTATATAAAT   841 ATAAAGTANT AAAAATTGAA CCATTANGAN TAGCACCCAC CNANGCAAAG AGAAGANTGG   901 TGCANANAGA AAAAAGANCA NNNGGAATAN GANCTTTGTT CCNTGGGNTC NNGGGNNCAG 961 CNNNAGCANT ANNGGCGNAG CNNNNNTNNN NNNGNNNNNA CNNNNNNNNN N //
Primer: bGH_rev3 (Pr0885) Region: 5' LTR Sequence file: RDB19614_B2Ead.seq
>D08836B1_B2Ea_1-bGH_rev3_D06_12_ABI24.ab1     1 NNNNNNNNNN NNNNGGNNGN NGGNNNNNNA CAGATGGCTG GCAACTAGAA GGCACAGTCG    61 AGGCTGNNNN NCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA CTAAAAGGGT   121 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT   181 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG   241 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC TCGGGCCATG   301 TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC CGGGTCATCC   361 ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT GGCTTCTTCT   421 ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG TGGATATCTG   481 ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT GTGGTAGATC   541 CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG TCCCCCCTTT   601 TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT TAAAGGTACC   661 TGANNTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA GGCACAATCA   721 GCATTGGTAG CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG GTCTCGACNG   781 TATCGATGCG GGGNNGCGGC CCAAAGGGAN NTCCGACTCG TCTGANGGCG AANGCGAAGA   841 CGCGGAANAN GCCGCAGANC CNGCAGCNNN CGCGGGAANG AANGNCCNCT GGNNTGAGGG   901 NCNAANNGNA NGTANCANNN NNNGTCCCGC GNANAATCCN NGTGGNNACN CNNNNANCAG 961 CCNNNGNAAA NGNANGTCNN CNTNNCCNGN NNNNNNNNN //
Primer: RRE_F1 (Pr0796) Region: RRE,cPPT_CTS Sequence file: RDB19614_B2Eae.seq
>D08836B1_B2Ea_1-RRE_F1_E06_15_ABI24.ab1     1 NNNNNNNNNN NNGNNNNNNN NNNNNNNNNN NNCNNANNNT TTGCTGNNGG CNATTGAGGC    61 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT   121 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA   181 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA   241 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT   301 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT   361 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA   421 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT   481 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT   541 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA   601 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTACAAAT GGCAGTATTC ATCCACAATT   661 TTAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA GACATAATAG   721 CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA AATTTTCGGG   781 TTTATTACAG GGACAGCAGA NATTCACTTT GAATTAATTC AAGCTTCGTG AGGCTCCNGT   841 GCCCGTCAGT GGGCAGANCG CACATCGCCC ACAGNCCCCG ANAANTTGGG GGGGANGGGT   901 CGGCNATTGA ACCGGTGCCT ANAGAANNNG GNGCGGGGTA AACNGGNNAA GTGANGNCNN   961 GNANTGGNTN CNCCNTTTTC CNNANGGNGG GGGANANNNN NNNATNNNNG CANTANTCNN 1021 NNNNNNNN //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro,cPPT_CTS Sequence file: RDB19614_B2Eaf.seq
>D08836B1_B2Ea_1-EF1a-N_R_F06_18_ABI24.ab1     1 NNNNNNNNNN NGNNNNNNNG GCNNNGTGCG CTCTGCCNNC TGACGGGCAC CGGNNCCTCA    61 CGAAGCTTGA ATTAATTCAA AGTGAATTTC TGCTGTCCCT GTAATAAACC CGAAAATTTT   121 GAATTTTTGT AATTTGTTTT TGTAATTCTT TAGTTTGTAT GTCTGTTGCT ATTATGTCTA   181 CTATTCTTTC CCCTGCACTG TACCCCCCAA TCCCCCCTTT TCTTTTAAAA TTGTGGATGA   241 ATACTGCCAT TTGTAGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC TCTCTCTCCA   301 CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG GTGGGTCTGA   361 AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC AGCAAAAACT   421 ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA CCACAGCCAA   481 TTTGTTATGT TAAACCAATT CCACAAACTT GCCCATTTAT CTAATTCCAA TAATTCTTGT   541 TCATTCTTTT CTTGCTGGTT TTGCGATTCT TCAATTAAGG AGTGTATTAA GCTTGTGTAA   601 TTGTTAATTT CTCTGTCCCA CTCCATCCAG GTCGTGTGAT TCCAAATCTG TTCCAGAGAT   661 TTATTACTCC AACTAGCATT CCAAGGCACA GCAGTGGTGC AAATGAGTTT TCCAGANCAA   721 CCCCAAATCC CCAGGAGCTG TTGATCCTTT ANGTATCTTT CCACAGCCAG GANTCTTGCC   781 TGGANCTGCT TGATGCCCCA GANTGTGAGT TGCAACAGAT GCTGTTGCGC CTCAATAGCC   841 NTCAGCAAAT TGTTCTGCTG CTGCACTATA CCAGACAATA ATTGTCTGGN CNGTACCGTC   901 AGCGTCATTG ANGCTGCGCC CATANTGCTN NCNGCTGCNN CCNAGANCCC NANGNAANAA   961 AGCTCCNATT CCCNCNNNTC NTTTTNNNNN NNGCNNNNNT NNNNNNNTTN NCNNNNNNNG 1021 NNNNNNNNNN //
Primer: SV40_F2 (Pr1194) Region: WPRE,5' LTR Sequence file: RDB19614_B2Eag.seq
>D08836B1_B2Ea_1-SV40_F2_G06_21_ABI24.ab1     1 NNNNNNNNNN NNNCNNNNNN TTNNGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC    61 CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGAGACCTA GAAAAACATG GAGCAATCAC   121 AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA   181 GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC   241 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA   301 ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTG   361 GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA   421 GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA ACACCCGCTT   481 GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT TAGAGTGGAG   541 GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG ACTGTACTGG   601 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT   661 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG   721 TGACTCTGGT AACTAGAGAT CCCTCAGANC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG   781 GGCCCGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG   841 TTTGCCCCTC CCCCGTGCCT TCCTTGANCC TGGAANGTGC CACTCCCACN NNCCTTNNCT   901 AATAAAATGA NGAAANTGCA TCGNATTNNC TGANTANGNG TCATTCNATT CTNGGGGGGT   961 GGGGTGGGGC NNNNAGCNNN GGGNNNNNNN NGNNNGNNNN TANCNNNNTG CNNGGGNNNN 1021 NNNNGNNNNN NNNGNNNNNN NNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,EM7 pro,Zeo Sequence file: RDB19614_B2Eah.seq
>D08836B1_B2Ea_1-SV40pro_ori_F_H06_24_ABI24.ab1     1 NNNNNNNNNN NNNNNGCCGC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT    61 TTTGGANGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT   121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA   181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG   241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG   301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG   361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT   421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA   481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT   541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG   601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC   661 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT   721 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC   781 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT   841 CNANCTCTAN CTAGANCTTG GCGTAATCAT GGNCATANCT GTTNCCNGTG TGAAATTGNT   901 ATCCGCTCNC AATTCCNCAC AACATACNAG CNNGAAGCAT AAAGTGNAAA GCCNGGGNNG   961 NCNAANGANT GANCTAACTC NNATTNNNNG CGTTGNNNNN NNNGNCCNNN TTNCANNNNN 1021 NNAANNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl