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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

KikGR/pRSETB

Photoswitchable green fluorescent protein, Kikume Green-Red (KikGR).

Catalog number RDB15259
Resource name KikGR/pRSETB
Clone info. Kikume Green-Red (KikGR), tetramer. It can be switched into red fluorescence upon illumination with UV or violet light.
Vector backbone pRSET B (Plasmid)
Gene/insert name Favia favus Kikume Green-Red (KikGR) fluorescent protein cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A. (2005) Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]
Remarks
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15259 KikGR/pRSETB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15259_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_-20bp
Sequence file: RDB15259_A7H9a.seq check
>D04498G8-2_A7H9_T7_20bp_G12_21_ABI24.ab1
    1 GCGTAACCCG TCTAGGATAA TTTTGTTTAC TGTTAAGAAG GAGATATACA TATGCGGGGT
   61 TCTCATCATC ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG
  121 GATCTGTACG ACGATGACGA TAAGGATCCG AGCGGCCGCA TGAGTGTGAT TACATCAGAA
  181 ATGAAGATCG AGCTGCGTAT GGAAGGCGCT GTAAACGGGC ACAAGTTCGT GATTACAGGG
  241 AAAGGAAGTG GCCAGCCTTT CGAGGGAATA CAGAATGTGG ACCTGACAGT CATAGAGGGC
  301 GGACCTCTTC CTTTTGCTTT CGATATCCTG ACAACAGCAT TCCATTACGG CAACCGGGTA
  361 TTTGTCGAAT ACCCAGAAGA AATAGTAGAC TACTTCAAGC AGTCGTTTCC TGAGGGTTAT
  421 TCTTGGGAAC GAAGCATGAG TTACGAAGAC GGGGGAATTT GCCTCGCCAC AAACAATATA
  481 ACGATGAAGA AAGACGGCAG CAACTGTTTT GTCAATGAAA TTCGATTTGA TGGTGTGAAC
  541 TTTCCTGCCA ATGGTCCAGT TATGCAGAGG AAGACCGTCA AATGGGAGCC ATCCACTGAG
  601 AAAATGTATG TGCGTGATGG AGTGCTGAAG GGTGATGTAA ACATGGCTCT GTTGCTTCAA
  661 GGAGGTGGCC ATTACCGATG TGACTTCAGA ACTACTTACA AAGCAAAGAA GGTTGTCCAG
  721 TTGCCAGACT ATCACTTCGT GGATCATCAA ATGGAGATAA CAAGCCATGA CAAGGATTAC
  781 AACAAGGTTA AGCTGTATGA GCATGCTAAA GCTCATTCCG GGCTGCCAAG GCTGGCCAAG
  841 TAACTCGAGA TCTGCAGCTG GTACCATGGA ATTCGAAGCT TGATCCGGCT GCTAACAAAG
  901 CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG
  961 GGGGCCTCTA ACGGGTCTTG AGGGGTTTTT TTTGCTGAAA GGAGACTATA TCCCGGATCT
 1021 GGGCGTAATA GCGTAGAGCC CGCAACGATC GGCCCTTCCA CCAGGTTGCC GCCAGCCTGA
1081 AATGCGATGG ACGCGCTCTG TAGCGGCCGC AATTTAAGGC GCCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765.

2019.12.08

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