Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

KikGR/pRSETB (#RDB15259)

Photoswitchable green fluorescent protein, Kikume Green-Red (KikGR).

Clone info. Kikume Green-Red (KikGR), tetramer. It can be switched into red fluorescence upon illumination with UV or violet light.
Vector backbone pRSET B (Plasmid)
Gene/insert name Favia favus Kikume Green-Red (KikGR) fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A. (2005) Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
宮脇敦史博士による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A. (2005) Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考 提供条件は英文のまま記載してください。
宮脇敦史博士の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15259 KikGR/pRSETB DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The KikGR/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15259).

Reference section:

Tsutsui, H., Karasawa, S., Shimizu, H., Nukina, N., Miyawaki, A., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15259_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp (Pr0261)
Region: RBS, 6XHis,T7 tag, Xprees tag, insert, MCS,T7 ter
Sequence file: RDB15259_A7H9a.seq check
>D04498G8-2_A7H9_T7_20bp_G12_21_ABI24.ab1
    1 GCGTAACCCG TCTAGGATAA TTTTGTTTAC TGTTAAGAAG GAGATATACA TATGCGGGGT
   61 TCTCATCATC ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG
  121 GATCTGTACG ACGATGACGA TAAGGATCCG AGCGGCCGCA TGAGTGTGAT TACATCAGAA
  181 ATGAAGATCG AGCTGCGTAT GGAAGGCGCT GTAAACGGGC ACAAGTTCGT GATTACAGGG
  241 AAAGGAAGTG GCCAGCCTTT CGAGGGAATA CAGAATGTGG ACCTGACAGT CATAGAGGGC
  301 GGACCTCTTC CTTTTGCTTT CGATATCCTG ACAACAGCAT TCCATTACGG CAACCGGGTA
  361 TTTGTCGAAT ACCCAGAAGA AATAGTAGAC TACTTCAAGC AGTCGTTTCC TGAGGGTTAT
  421 TCTTGGGAAC GAAGCATGAG TTACGAAGAC GGGGGAATTT GCCTCGCCAC AAACAATATA
  481 ACGATGAAGA AAGACGGCAG CAACTGTTTT GTCAATGAAA TTCGATTTGA TGGTGTGAAC
  541 TTTCCTGCCA ATGGTCCAGT TATGCAGAGG AAGACCGTCA AATGGGAGCC ATCCACTGAG
  601 AAAATGTATG TGCGTGATGG AGTGCTGAAG GGTGATGTAA ACATGGCTCT GTTGCTTCAA
  661 GGAGGTGGCC ATTACCGATG TGACTTCAGA ACTACTTACA AAGCAAAGAA GGTTGTCCAG
  721 TTGCCAGACT ATCACTTCGT GGATCATCAA ATGGAGATAA CAAGCCATGA CAAGGATTAC
  781 AACAAGGTTA AGCTGTATGA GCATGCTAAA GCTCATTCCG GGCTGCCAAG GCTGGCCAAG
  841 TAACTCGAGA TCTGCAGCTG GTACCATGGA ATTCGAAGCT TGATCCGGCT GCTAACAAAG
  901 CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG
  961 GGGGCCTCTA ACGGGTCTTG AGGGGTTTTT TTTGCTGAAA GGAGACTATA TCCCGGATCT
 1021 GGGCGTAATA GCGTAGAGCC CGCAACGATC GGCCCTTCCA CCAGGTTGCC GCCAGCCTGA
1081 AATGCGATGG ACGCGCTCTG TAGCGGCCGC AATTTAAGGC GCCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. [link to RRC of NBRP]