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mMiCy1/pRSETB

Plasmid clone of fluorescent protein monomeric Midoriishi-Cyan (mMiCy1).

Catalog number RDB15241
Resource name mMiCy1/pRSETB
Clone info. Plasmid clone of fluorescent protein monomeric Midoriishi-Cyan (mMiCy1).
Vector backbone Plasmid
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]
Remarks
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15241 mMiCy1/pRSETB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15241_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15241_A7H9a.seq
>D04498E8-2_A7H9_T7_20bp_D07_10_ABI24.ab1
    1 GAAATTTCGC TGGATAATTT TGTTTACTTT AAGAAGGAGA TATACATATG CGGGGTTCTC
   61 ATCATCATCA TCATCATGGT ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGGGATC
  121 TGTACGACGA TGACGATAAG GATCCGACCA TGGTGTCTTA TTCAAAGCAA GGCATCGCAC
  181 AAGAAATGCG GACGAAATAC CGTATGGAAG GCAGTGTCAA TGGCCATGAG TTCACGATCG
  241 AAGGTGTAGG AACTGGAAAC CCTTACGAAG GGAAACAGAC GTCCGAATTA GTGATCATCA
  301 AGCCTAAGGG AAAACCCCTT CCATTCTCCT TTGACATACT GTCAACAGTC TTTCAATATG
  361 GAAACAGATG CTTCACAAAG TACCCTGCCG ACATGCCTGA CTATTTCAAG CAAGCATTCC
  421 CAGATGGAAT GTCATATGAA AGGTCATTTC TATTTGAGGA TGGAGGAGTT GCTACAGCCA
  481 GCTGGAGCAT TCGTCTCGAA GGAAATTGCT TCATCCACAA TTCCATCTAT CATGGCACAA
  541 ACTTTCCCGC TGATGGACCC GTAATGAAGA AGCAGACAAT TGGCTGGGAT AAGTCCTCCG
  601 AAAAAATGAG TGTGGCTAAA GAGGTGCTAA GAGGTGATGT GACTCAGTTT CTTCTGCTCG
  661 AAGGAGGTGG TTACCAGAGA TGCCAGTTAC ACTCCACTTA CAAAACAGAG AAGCCAGTCG
  721 CAATGCCCCC GAGTCATGTC GTAGAACATC AAATTGTGAG GACCGACCTT GGCCAAACTG
  781 CAAAAGGCTT CAAGGTCAAG CTGGAAGAAC ATGCTGAGGC TCATGTTAAC CCTTTGAAGG
  841 TTAAATAACT CTTTTCAAGA TGGAAGCACT TTCAATACTT ACAATCAACG TCTTGAATTC
  901 GAAGCTTGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC
  961 TGAGCAATAA CTAGCATAAC CCCTTTGGGG CCTCTAAACG GTCTTGAGGG GTTTTTTGCT
 1021 GAAAGGAGGA CTATATCCGG ATCTGCGTAA TAGCCGAGAG CCGGCACGAT CGCCTTCTAC
1081 AGTGCGCAGC CTGTATGACG ATTGGGGGAC GCGCCCCTGG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

2019.07.30

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