Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mMiCy1/pRSETB (#RDB15241)

Plasmid clone of fluorescent protein monomeric Midoriishi-Cyan (mMiCy1).

Clone info. Plasmid clone of fluorescent protein monomeric Midoriishi-Cyan (mMiCy1).
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15241 mMiCy1/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mMiCy1/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15241).

Reference section:

Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15241_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp ()
Sequence file: RDB15241_A7H9a.seq check
>D04498E8-2_A7H9_T7_20bp_D07_10_ABI24.ab1
    1 GAAATTTCGC TGGATAATTT TGTTTACTTT AAGAAGGAGA TATACATATG CGGGGTTCTC
   61 ATCATCATCA TCATCATGGT ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGGGATC
  121 TGTACGACGA TGACGATAAG GATCCGACCA TGGTGTCTTA TTCAAAGCAA GGCATCGCAC
  181 AAGAAATGCG GACGAAATAC CGTATGGAAG GCAGTGTCAA TGGCCATGAG TTCACGATCG
  241 AAGGTGTAGG AACTGGAAAC CCTTACGAAG GGAAACAGAC GTCCGAATTA GTGATCATCA
  301 AGCCTAAGGG AAAACCCCTT CCATTCTCCT TTGACATACT GTCAACAGTC TTTCAATATG
  361 GAAACAGATG CTTCACAAAG TACCCTGCCG ACATGCCTGA CTATTTCAAG CAAGCATTCC
  421 CAGATGGAAT GTCATATGAA AGGTCATTTC TATTTGAGGA TGGAGGAGTT GCTACAGCCA
  481 GCTGGAGCAT TCGTCTCGAA GGAAATTGCT TCATCCACAA TTCCATCTAT CATGGCACAA
  541 ACTTTCCCGC TGATGGACCC GTAATGAAGA AGCAGACAAT TGGCTGGGAT AAGTCCTCCG
  601 AAAAAATGAG TGTGGCTAAA GAGGTGCTAA GAGGTGATGT GACTCAGTTT CTTCTGCTCG
  661 AAGGAGGTGG TTACCAGAGA TGCCAGTTAC ACTCCACTTA CAAAACAGAG AAGCCAGTCG
  721 CAATGCCCCC GAGTCATGTC GTAGAACATC AAATTGTGAG GACCGACCTT GGCCAAACTG
  781 CAAAAGGCTT CAAGGTCAAG CTGGAAGAAC ATGCTGAGGC TCATGTTAAC CCTTTGAAGG
  841 TTAAATAACT CTTTTCAAGA TGGAAGCACT TTCAATACTT ACAATCAACG TCTTGAATTC
  901 GAAGCTTGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC
  961 TGAGCAATAA CTAGCATAAC CCCTTTGGGG CCTCTAAACG GTCTTGAGGG GTTTTTTGCT
 1021 GAAAGGAGGA CTATATCCGG ATCTGCGTAA TAGCCGAGAG CCGGCACGAT CGCCTTCTAC
1081 AGTGCGCAGC CTGTATGACG ATTGGGGGAC GCGCCCCTGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]