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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mMiCy1/pRSETB

Plasmid clone of fluorescent protein monomeric Midoriishi-Cyan (mMiCy1).

Catalog number RDB15241
Resource name mMiCy1/pRSETB
Clone info. Plasmid clone of fluorescent protein monomeric Midoriishi-Cyan (mMiCy1).
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
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External Database
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15241 mMiCy1/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The mMiCy1/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15241).

            Reference section:

            Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

            Tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15241_A7H9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp ()
            Sequence file: RDB15241_A7H9a.seq check
            >D04498E8-2_A7H9_T7_20bp_D07_10_ABI24.ab1
                1 GAAATTTCGC TGGATAATTT TGTTTACTTT AAGAAGGAGA TATACATATG CGGGGTTCTC
               61 ATCATCATCA TCATCATGGT ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGGGATC
              121 TGTACGACGA TGACGATAAG GATCCGACCA TGGTGTCTTA TTCAAAGCAA GGCATCGCAC
              181 AAGAAATGCG GACGAAATAC CGTATGGAAG GCAGTGTCAA TGGCCATGAG TTCACGATCG
              241 AAGGTGTAGG AACTGGAAAC CCTTACGAAG GGAAACAGAC GTCCGAATTA GTGATCATCA
              301 AGCCTAAGGG AAAACCCCTT CCATTCTCCT TTGACATACT GTCAACAGTC TTTCAATATG
              361 GAAACAGATG CTTCACAAAG TACCCTGCCG ACATGCCTGA CTATTTCAAG CAAGCATTCC
              421 CAGATGGAAT GTCATATGAA AGGTCATTTC TATTTGAGGA TGGAGGAGTT GCTACAGCCA
              481 GCTGGAGCAT TCGTCTCGAA GGAAATTGCT TCATCCACAA TTCCATCTAT CATGGCACAA
              541 ACTTTCCCGC TGATGGACCC GTAATGAAGA AGCAGACAAT TGGCTGGGAT AAGTCCTCCG
              601 AAAAAATGAG TGTGGCTAAA GAGGTGCTAA GAGGTGATGT GACTCAGTTT CTTCTGCTCG
              661 AAGGAGGTGG TTACCAGAGA TGCCAGTTAC ACTCCACTTA CAAAACAGAG AAGCCAGTCG
              721 CAATGCCCCC GAGTCATGTC GTAGAACATC AAATTGTGAG GACCGACCTT GGCCAAACTG
              781 CAAAAGGCTT CAAGGTCAAG CTGGAAGAAC ATGCTGAGGC TCATGTTAAC CCTTTGAAGG
              841 TTAAATAACT CTTTTCAAGA TGGAAGCACT TTCAATACTT ACAATCAACG TCTTGAATTC
              901 GAAGCTTGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC
              961 TGAGCAATAA CTAGCATAAC CCCTTTGGGG CCTCTAAACG GTCTTGAGGG GTTTTTTGCT
             1021 GAAAGGAGGA CTATATCCGG ATCTGCGTAA TAGCCGAGAG CCGGCACGAT CGCCTTCTAC
            1081 AGTGCGCAGC CTGTATGACG ATTGGGGGAC GCGCCCCTGG
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

            2023.08.17

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