RDB15243 - cjBlue Y64L/pRSETB

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

cjBlue Y64L/pRSETB (#RDB15243)

Plasmid clone of yellow color protein, a variant of cjBlue of sea anemone Cnidopus japonicus.

Clone info.	Plasmid clone of yellow color protein, a variant of cjBlue. Bacterial expression.
Vector backbone	pRSETB (plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Amp^r
Gene/insert name	Cnidopus japonicus blue color protein cDNA
Reference of insert sequence	BD408956 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Sequence (full)	RDB15243zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Prive, G.G., Miyawaki, A., Ikura, M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Prive, G.G., Miyawaki, A., Ikura, M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Prive, G.G., Miyawaki, A., Ikura, M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15243	cjBlue Y64L/pRSETB	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The cjBlue Y64L/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15243).

Reference section:

Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Privé, G.G., Miyawaki, A., Ikura, M., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15243_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724) Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB15243_A7H9a.seq
>15243_B0L4F_1_lacIpro-B_F_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNNNCGCNN CGNGTCNGTG AGCGAGGAAG CGGAAGANCG CCCAATACGC 61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT 121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC 181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA 241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGA 301 CCATGGCTTC CAAAATCAGC GACAATGTAC GTATCAAGTT ATATATGGAG GGCACAGTCA 361 ACAATCATCA CTTCATGTGC GAAGCTGAAG GAGAGGGCAA GCCATACGAG GGAACTCAAA 421 TGGAGAACAT AAAAGTCACC AAAGGAGGCC CTCTGCCGTT CTCTTTTGAT ATCTTGACGC 481 CTAACTGCCA ACTCGGAAGC GTAGCCATAA CCAAGTATAC ATCAGGGATT CCAGACTACT 541 TTAAGCAATC TTTTCCTGAA GGATTTACCT GGGAAAGAAC CACAATCTAC GAAGATGGGG 601 CTTACCTTAC AACTCAACAA GAAACCAAAC TTGATGGAAA TTGCCTCGTC TACAATATTA 661 AAATCCTTGG ATGTAATTTT CCCCCCAATG GTCCTGTGAT GCAGAAGAAA ACCCAAGGCT 721 GGGAACCCTG TTGCGAGATG CGCTATACAC GTGATGGTGT GCTATGTGGC CAAACATTAA 781 TGGCACTTAA ATGCGCCGAT GGGAACCACC TCACTTGCCA TCTGAGAACT ACTTACAGGT 841 CCNAAAAGGC AGCAAAGGCG TTGCAGATGC CACCCTTCCN TTTTTCANAC CATCGNCCTG 901 AAATAGTGAA NGNTTCANAN AACGGNNCAC TATTTGAACA GCANGAANGT TCAGNGGCCA 961 GGTACTGNCN AACATGCCCA TCNAAACTNG GNCACNATTA AANGNANNAC ANNANNNNCN 1021 TTNCNNNCNN NNNNNAACTT NNNTTNNNNN NNNNTTTTNN NNNNTTTNNC NNNNNNNNNN //
Primer: cjB-M_F1 (Pr0958) Region: insert 3' Sequence file: RDB15243_A7H9b.seq
>15243_B0L4F_1_cjB-M_F1_B02_02_ABI08.ab1 1 NNNNNNNNNN NNNNNGNNNN NNNNNNNNCC CNCCAATGGT CCTGTGNTGC AGAAGAAAAC 61 CCAAGGCTGG GAACCCTGTT GCGAGATGCG CTATACACGT GATGGTGTGC TATGTGGCCA 121 AACATTAATG GCACTTAAAT GCGCCGATGG GAACCACCTC ACTTGCCATC TGAGAACTAC 181 TTACAGGTCC AAAAAGGCAG CAAAGGCGTT GCAGATGCCA CCCTTCCATT TTTCAGACCA 241 TCGTCCTGAA ATAGTGAAGG TTTCAGAGAA CGGCACACTA TTTGAACAGC ACGAAAGTTC 301 AGTGGCCAGG TACTGTCAAA CATGCCCATC TAAACTTGGT CACAATTAAA GGACAACACT 361 AGCTTCATTC CAACCAGGAC AAACTTAGTT TCATGGATAT TTTTAATAGT TTTTCCCTAT 421 CAGTGTCCGA TTCCTAATTC CAATTCCTAA CTAGATATTT CATTTGAATT GTCATTGATT 481 TATATGGCTG TAACATTGTG CATGCAACAA TTGTTTTCTG AGATTGATTG TGTCTTTGAA 541 ATCAATAATA AACTCATACT TTTAAATTAA AAAAAAAAAA AAAAAAAAAA AAAAAATTCC 601 TGCGGCCGCG AATTCNAAGC TTGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGANTT 661 GGCTGCTGCC ACCGCTGANC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT 721 GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATCT GGCGTAATAG CNAANAGGCC 781 CGCACCGATC GCCCTTCCCA ACAGTTGCGC ANCCTGANTG GNGAATGGGA CGCGCCCTGT 841 ANCGNCNNAT TANGCGCGGC GGGNGNGGNG GNTACNCGCA GCGNNNNNGC TACACNNGNN 901 AGCGCCCTAN CNCCNGCTCC TTTCNNTTNT TNCCTTCCTT TCNCGCNNCG TTCGCNGGNT 961 TTNCCCGNNN AGNTCTAANT CNGGGNNNNC NTTTNNNNNN CNANTNNNNG NTTNNNNNNN 1021 NCTCNNNCCN NNNNANNNGN NNNGNNGANG NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187. [link to RRC of NBRP]

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External Database
Cnidopus japonicus blue color protein

Reference sequence