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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

cjBlue Y64L/pRSETB (#RDB15243)

Plasmid clone of yellow color protein, a variant of cjBlue of sea anemone Cnidopus japonicus.

Clone info. Plasmid clone of yellow color protein, a variant of cjBlue. Bacterial expression.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC.
Gene/insert name Cnidopus japonicus blue color protein cDNA
Reference of insert sequence BD408956 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15243zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Chan, M.C. et al., J. Biol. Chem. 281 (49): 37813-37819 (2006).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Chan, M.C. et al., J. Biol. Chem. 281 (49): 37813-37819 (2006).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15243 cjBlue Y64L/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The cjBlue Y64L/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15243).

Reference section:

Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Privé, G.G., Miyawaki, A., Ikura, M., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15243_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15243_A7H9a.seq check
>15243_B0L4F_1_lacIpro-B_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNCGCNN CGNGTCNGTG AGCGAGGAAG CGGAAGANCG CCCAATACGC
   61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT
  121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC
  181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA
  241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGA
  301 CCATGGCTTC CAAAATCAGC GACAATGTAC GTATCAAGTT ATATATGGAG GGCACAGTCA
  361 ACAATCATCA CTTCATGTGC GAAGCTGAAG GAGAGGGCAA GCCATACGAG GGAACTCAAA
  421 TGGAGAACAT AAAAGTCACC AAAGGAGGCC CTCTGCCGTT CTCTTTTGAT ATCTTGACGC
  481 CTAACTGCCA ACTCGGAAGC GTAGCCATAA CCAAGTATAC ATCAGGGATT CCAGACTACT
  541 TTAAGCAATC TTTTCCTGAA GGATTTACCT GGGAAAGAAC CACAATCTAC GAAGATGGGG
  601 CTTACCTTAC AACTCAACAA GAAACCAAAC TTGATGGAAA TTGCCTCGTC TACAATATTA
  661 AAATCCTTGG ATGTAATTTT CCCCCCAATG GTCCTGTGAT GCAGAAGAAA ACCCAAGGCT
  721 GGGAACCCTG TTGCGAGATG CGCTATACAC GTGATGGTGT GCTATGTGGC CAAACATTAA
  781 TGGCACTTAA ATGCGCCGAT GGGAACCACC TCACTTGCCA TCTGAGAACT ACTTACAGGT
  841 CCNAAAAGGC AGCAAAGGCG TTGCAGATGC CACCCTTCCN TTTTTCANAC CATCGNCCTG
  901 AAATAGTGAA NGNTTCANAN AACGGNNCAC TATTTGAACA GCANGAANGT TCAGNGGCCA
  961 GGTACTGNCN AACATGCCCA TCNAAACTNG GNCACNATTA AANGNANNAC ANNANNNNCN
 1021 TTNCNNNCNN NNNNNAACTT NNNTTNNNNN NNNNTTTTNN NNNNTTTNNC NNNNNNNNNN
//
Primer: cjB-M_F1 (Pr0958)
Region: insert 3'
Sequence file: RDB15243_A7H9b.seq check
>15243_B0L4F_1_cjB-M_F1_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNGNNNN NNNNNNNNCC CNCCAATGGT CCTGTGNTGC AGAAGAAAAC
   61 CCAAGGCTGG GAACCCTGTT GCGAGATGCG CTATACACGT GATGGTGTGC TATGTGGCCA
  121 AACATTAATG GCACTTAAAT GCGCCGATGG GAACCACCTC ACTTGCCATC TGAGAACTAC
  181 TTACAGGTCC AAAAAGGCAG CAAAGGCGTT GCAGATGCCA CCCTTCCATT TTTCAGACCA
  241 TCGTCCTGAA ATAGTGAAGG TTTCAGAGAA CGGCACACTA TTTGAACAGC ACGAAAGTTC
  301 AGTGGCCAGG TACTGTCAAA CATGCCCATC TAAACTTGGT CACAATTAAA GGACAACACT
  361 AGCTTCATTC CAACCAGGAC AAACTTAGTT TCATGGATAT TTTTAATAGT TTTTCCCTAT
  421 CAGTGTCCGA TTCCTAATTC CAATTCCTAA CTAGATATTT CATTTGAATT GTCATTGATT
  481 TATATGGCTG TAACATTGTG CATGCAACAA TTGTTTTCTG AGATTGATTG TGTCTTTGAA
  541 ATCAATAATA AACTCATACT TTTAAATTAA AAAAAAAAAA AAAAAAAAAA AAAAAATTCC
  601 TGCGGCCGCG AATTCNAAGC TTGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGANTT
  661 GGCTGCTGCC ACCGCTGANC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT
  721 GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATCT GGCGTAATAG CNAANAGGCC
  781 CGCACCGATC GCCCTTCCCA ACAGTTGCGC ANCCTGANTG GNGAATGGGA CGCGCCCTGT
  841 ANCGNCNNAT TANGCGCGGC GGGNGNGGNG GNTACNCGCA GCGNNNNNGC TACACNNGNN
  901 AGCGCCCTAN CNCCNGCTCC TTTCNNTTNT TNCCTTCCTT TCNCGCNNCG TTCGCNGGNT
  961 TTNCCCGNNN AGNTCTAANT CNGGGNNNNC NTTTNNNNNN CNANTNNNNG NTTNNNNNNN
1021 NCTCNNNCCN NNNNANNNGN NNNGNNGANG NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187. [link to RRC of NBRP]