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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

cjBlue Y64L/pRSETB

Plasmid clone of yellow color protein, a variant of cjBlue of sea anemone Cnidopus japonicus.

Catalog number RDB15243
Resource name cjBlue Y64L/pRSETB
Clone info. Plasmid clone of yellow color protein, a variant of cjBlue. Bacterial expression.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Cnidopus japonicus blue color protein cDNA
Reference of nucleotide sequence BD408956 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Prive, G.G., Miyawaki, A., Ikura, M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15243 cjBlue Y64L/pRSETB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence compiled by us RDB15243zzt01.seq

Original reference

original Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15243_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15243_A7H9a.seq check
>15243_B0L4F_1_lacIpro-B_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNCGCNN CGNGTCNGTG AGCGAGGAAG CGGAAGANCG CCCAATACGC
   61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT
  121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC
  181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA
  241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGA
  301 CCATGGCTTC CAAAATCAGC GACAATGTAC GTATCAAGTT ATATATGGAG GGCACAGTCA
  361 ACAATCATCA CTTCATGTGC GAAGCTGAAG GAGAGGGCAA GCCATACGAG GGAACTCAAA
  421 TGGAGAACAT AAAAGTCACC AAAGGAGGCC CTCTGCCGTT CTCTTTTGAT ATCTTGACGC
  481 CTAACTGCCA ACTCGGAAGC GTAGCCATAA CCAAGTATAC ATCAGGGATT CCAGACTACT
  541 TTAAGCAATC TTTTCCTGAA GGATTTACCT GGGAAAGAAC CACAATCTAC GAAGATGGGG
  601 CTTACCTTAC AACTCAACAA GAAACCAAAC TTGATGGAAA TTGCCTCGTC TACAATATTA
  661 AAATCCTTGG ATGTAATTTT CCCCCCAATG GTCCTGTGAT GCAGAAGAAA ACCCAAGGCT
  721 GGGAACCCTG TTGCGAGATG CGCTATACAC GTGATGGTGT GCTATGTGGC CAAACATTAA
  781 TGGCACTTAA ATGCGCCGAT GGGAACCACC TCACTTGCCA TCTGAGAACT ACTTACAGGT
  841 CCNAAAAGGC AGCAAAGGCG TTGCAGATGC CACCCTTCCN TTTTTCANAC CATCGNCCTG
  901 AAATAGTGAA NGNTTCANAN AACGGNNCAC TATTTGAACA GCANGAANGT TCAGNGGCCA
  961 GGTACTGNCN AACATGCCCA TCNAAACTNG GNCACNATTA AANGNANNAC ANNANNNNCN
 1021 TTNCNNNCNN NNNNNAACTT NNNTTNNNNN NNNNTTTTNN NNNNTTTNNC NNNNNNNNNN
//
Primer: cjB-M_F1
Region: insert 3'
Sequence file: RDB15243_A7H9b.seq check
>15243_B0L4F_1_cjB-M_F1_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNGNNNN NNNNNNNNCC CNCCAATGGT CCTGTGNTGC AGAAGAAAAC
   61 CCAAGGCTGG GAACCCTGTT GCGAGATGCG CTATACACGT GATGGTGTGC TATGTGGCCA
  121 AACATTAATG GCACTTAAAT GCGCCGATGG GAACCACCTC ACTTGCCATC TGAGAACTAC
  181 TTACAGGTCC AAAAAGGCAG CAAAGGCGTT GCAGATGCCA CCCTTCCATT TTTCAGACCA
  241 TCGTCCTGAA ATAGTGAAGG TTTCAGAGAA CGGCACACTA TTTGAACAGC ACGAAAGTTC
  301 AGTGGCCAGG TACTGTCAAA CATGCCCATC TAAACTTGGT CACAATTAAA GGACAACACT
  361 AGCTTCATTC CAACCAGGAC AAACTTAGTT TCATGGATAT TTTTAATAGT TTTTCCCTAT
  421 CAGTGTCCGA TTCCTAATTC CAATTCCTAA CTAGATATTT CATTTGAATT GTCATTGATT
  481 TATATGGCTG TAACATTGTG CATGCAACAA TTGTTTTCTG AGATTGATTG TGTCTTTGAA
  541 ATCAATAATA AACTCATACT TTTAAATTAA AAAAAAAAAA AAAAAAAAAA AAAAAATTCC
  601 TGCGGCCGCG AATTCNAAGC TTGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGANTT
  661 GGCTGCTGCC ACCGCTGANC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT
  721 GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATCT GGCGTAATAG CNAANAGGCC
  781 CGCACCGATC GCCCTTCCCA ACAGTTGCGC ANCCTGANTG GNGAATGGGA CGCGCCCTGT
  841 ANCGNCNNAT TANGCGCGGC GGGNGNGGNG GNTACNCGCA GCGNNNNNGC TACACNNGNN
  901 AGCGCCCTAN CNCCNGCTCC TTTCNNTTNT TNCCTTCCTT TCNCGCNNCG TTCGCNGGNT
  961 TTNCCCGNNN AGNTCTAANT CNGGGNNNNC NTTTNNNNNN CNANTNNNNG NTTNNNNNNN
1021 NCTCNNNCCN NNNNANNNGN NNNGNNGANG NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187.

2021.02.18

GNP_filter3_RDBDEP_html_201222.pl