Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

SRAI_pcDNA3

Expression vector of fluorescent protein, SRAI (signal-retaining autophagy indicator) for autophagy sensor.

Catalog number RDB18222
Resource name SRAI_pcDNA3
Alternative name pcDNA3/SRAI
Clone info. Expression vector of fluorescent protein, SRAI (signal-retaining autophagy indicator) for autophagy sensor. SRAI is a tandem fusion of YPet and TOLLES, fused with a DNA linker that encodes a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser.
The accession number in the DDBJ/EMBL/GenBank databases is [LC491575]. Ypet was created by introducing mutations into EYFP. TOLLES was cloned by introducing mutations into monomeric Azami Green (MBL). Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells)
Gene/insert name Aequorea victoria GFP cDNA Galaxea fascicularis GFP cDNA
Reference of nucleotide sequence LC491575 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Katayama, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Katayama H. et al. Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. (2020). Cell 181(5), 1176-1187.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18222 SRAI_pcDNA3 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB18222pka01.seq

Original reference

original Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18222_B0Ffp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: insert 5'
Sequence file: RDB18222_B0Ffa.seq check
>D07201A1_B0Ef_1_CMV_Forward_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN TGNNGCAAAT GGGCGGTANG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTCTCTNGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
  121 TACGACTCAC TATAGGGAGA CCCAAGCTTG GTACCGAGCT CGGATCCCAC CATGGTGAGC
  181 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  241 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  301 ACCCTGAAGC TGCTCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  361 ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC
  421 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  481 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  541 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  601 TACAACTACA ACAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG
  661 GCCAACTTCA AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGANCACTAC
  721 CAGCAGAACA CCCCCATCNG CGACGGCCCC GTGCTGCTGC CCGANNACCA CTACCTGANC
  781 TACCAGTCCG CCCTGTTCAA AGACCCCAAC GANAANCGCG NTCACATGGT CCTGCTGGAN
  841 TNCCTGANCG CCGCCGGGAT CACTGAANGN ATGAANNGAN CTGNANNAAN GANCTCNGNN
  901 GGANGNNNTN NNNNNNNNGT GGCTCTNGNG GNGNNNNATC GNTCNNNATG GNNANCNNGN
961 TNNANNNNNN NNNN
//
Primer: f1ori_R3
Region: BGH p(A), insert 3'
Sequence file: RDB18222_B0Ffb.seq check
>D07201A1_B0Ef_1_f1ori_R3_B05_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TCTTTCCGCC TCNNAANCCN TAGAGCCCAC
   61 CGCATCCCCA GCATGCCTGC TATTGTCTTC CCAATCCTCC CCCTTGCTGT CCTGCCCCAC
  121 CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC GATGCAATTT CCTCATTTTA
  181 TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG GAAGGCACGG GGGAGGGGCA
  241 AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT GATCAGCGAG CTCTAGCATT
  301 TAGGTGACAC TATAGAATAG GGCCCTCTAG ATGCATGCTC GAGCGGCCGC CAGTGTGATG
  361 GATATCTGCA GAATTCGCTA GCTCACTTGG CCTGGCTGGG CAGCATGGAG GCCCTGGCCA
  421 CGGCGTTCTC GCACAGCTTC ACCTTGTTGT AGTCCTTGTC GTGCTCCAGG ATCTCGATCC
  481 TGTGGTCGAT CTTGTGGGCG TCGGGCAGGC TCACCTCCTT CTTGGCCTTG TAGGTGGTCT
  541 TGACGTCGCA CCTGTAGTGG CCGCCGCCCT CCAGCAGCAG CCTCATGTTC ACGTCGCCCT
  601 TCAGCACGCC GTCCTCCACG TACATCTTCT CGGTGTCGGG CTCCCACTTC AGGGTCTTCT
  661 TCTGCATCAC GGGGCCGTTG GGGGGGAAGT TGGTGCCGTC GAACCTGATG TCGTANAANA
  721 ANCAGTCGCC CCTCATGCTG ATGTTGCTGG TGGCGGTGCA GATGCCCTGG TCCTCGTANG
  781 TCATGCTCCT CTCCCAGTGG TAGCCCTCGG GGAANGNCTG CTTGAAGTAN TCCTGGATGT
  841 CGGCGGGGNN NTTGGNGAAN GNCNNGTTGC CGTACTGGAN CNANGNGGAN AGGATGTCGN
  901 NNNNNAANGG CAGGGGGGCN NCNTCNGNCA CGNTTCNNNT NCNNGNNCTG GGNGNCNNNN
961 NNNGGGNTNG CCCTNNCCNN CNNCCNNNNN NCACNNANNN TNNNNNNNNN N
//
Primer: mVenus-M_F
Region: insert mid
Sequence file: RDB18222_B0Ffc.seq check
>D07201A1_B0Ff_1_mVenus-M_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNANNNANGN NNTCNNGGCC NACTTCAAGA NCCGCCNCAA CATCGAGGAC
   61 GGCGGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG
  121 CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCGCCC TGTTCAAAGA CCCCAACGAG
  181 AAGCGCGATC ACATGGTCCT GCTGGAGTTC CTGACCGCCG CCGGGATCAC TGAAGGCATG
  241 AACGAGCTGT ACAAGGAGCT CGGTGGAGGC GGTTCAGGCG GAGGTGGCTC TGGCGGTGGC
  301 GGATCGCTCG AGATGGTGAG CGTGATCAAG CCCGAGATGA AGATCAAGCT GTGCATGAGG
  361 GGCACCGTGA ACGGCCACAA CTTCGTGATT GAGGGCGAGG GCAAGGGCAA CCCCTACGAG
  421 GGCACCCAGA TCCTGGACCT GAACGTGACC GAGGGCGCCC CCCTGCCCTT CGCCTACGAC
  481 ATCCTGTCCA CCTCGTTCCA GTACGGCAAC AGGGCCTTCA CCAAGTACCC CGCCGACATC
  541 CAGGACTACT TCAAGCAGGC CTTCCCCGAG GGCTACCACT GGGAGAGGAG CATGACCTAC
  601 GAGGACCAGG GCATCTGCAC CGCCACCAGC AACATCAGCA TGAGGGGCGA CTGCTTCTTC
  661 TACGACATCA GGTTCGACGG CACCNACTTC CCCCCCAACG GCCCCGTGAT GCAGAAGAAG
  721 ANCCTGAAGT GGGAGCCCGA CACCGANAAG ATGTACGTGG AGGANGGCGT GCTGAAGGGC
  781 GACGTGAACA TGAGGCTGCT GCTGGANGGC GGCGGCCACT ACAGGTGCNA CGTCANNACC
  841 ACCTACNNNN CCNAGAANGA NGTGAGCCTG CCCNANNNCC ACAAGATCGA CCACAGGATN
  901 NANATNCTGG ANCACGANNN NGNNTANNNC NAGGNGANNC TGNNCNANAA CNCCGNNNNN
  961 NNGGGNNTNN NNGCNNCCNN NCCNGNNNNA NNNNNNCNNN NNNNNTNNNN NNNANNNNNN
1021 NNNN
//
Primer: SV40pro_ori_F
Region: SV40pro_ori, NeoR
Sequence file: RDB18222_B0Ffd.seq check
>D07201A1_B0Ff_1_SV40pro_ori_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNNNNTCTG CCTCTGANCT ATTCNNGAAG TAGTGAGGAG
   61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
  121 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
  181 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
  241 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
  301 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
  361 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
  421 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
  481 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
  541 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
  601 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
  661 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA NGNTCTCGTC GTGACCCATG
  721 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
  781 GTGGCCGGCT GGGTGTGGCG GANCGCTATC ANGACATANC GTTGGCTACC CGNGATATTG
  841 CTGAAGANCT NGGCGGCNAA NGGGCTGANC GCTTCCNCNN GCTTTACNGT ATCGCCNCTC
  901 CNGANNNNCA NCNNANCGCC TTCNNTCNNC NNNNNNNNNA NNNNNNCNNA NCNGGNNTCT
961 GGGGGNTNCN NANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660.

2021.01.07

GNP_filter3_RDBDEP_html_201222.pl