RDB18222 - SRAI

Expression vector of fluorescent protein, SRAI (signal-retaining autophagy indicator) for autophagy sensor.

Distribution information

Catalog number	RDB18222
Resource name	SRAI_pcDNA3
Alternative name	pcDNA3/SRAI
Clone info.	Expression vector of fluorescent protein, SRAI (signal-retaining autophagy indicator) for autophagy sensor. SRAI is a tandem fusion of YPet and TOLLES, fused with a DNA linker that encodes a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser. The accession number in the DDBJ/EMBL/GenBank databases is [LC491575]. Ypet was created by introducing mutations into EYFP. TOLLES was cloned by introducing mutations into monomeric Azami Green (MBL). Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone	pcDNA3 (plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Ampicillin (E. coli), Neomycin (mammalian cells)
Gene/insert name	Aequorea victoria GFP cDNA Galaxea fascicularis GFP cDNA
Reference of nucleotide sequence	LC491575 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \| Katayama, Hiroyuki \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama H. et al. Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. (2020). Cell 181(5), 1176-1187. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please use Schedule A (GFP-A) additionally. Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users)

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提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama H. et al. Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. (2020). Cell 181(5), 1176–1187. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 Schedule A（GFP-A, 英語版のみ）が追加で必要です。宮脇敦史博士の書面による提供承諾が必要です。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18222	SRAI_pcDNA3	DNA solution

Electronic file	Nucleotide sequence (partial) provided by the depositor RDB18222pka01.seq

original	Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660.

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: CMV_Forward Region: insert 5' Sequence file: RDB18222_B0Ffa.seq
>D07201A1_B0Ef_1_CMV_Forward_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN TGNNGCAAAT GGGCGGTANG CGTGTACGGT GGGAGGTCTA 61 TATAAGCAGA GCTCTCTNGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA 121 TACGACTCAC TATAGGGAGA CCCAAGCTTG GTACCGAGCT CGGATCCCAC CATGGTGAGC 181 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA 241 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG 301 ACCCTGAAGC TGCTCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC 361 ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC 421 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC 481 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC 541 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG 601 TACAACTACA ACAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG 661 GCCAACTTCA AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGANCACTAC 721 CAGCAGAACA CCCCCATCNG CGACGGCCCC GTGCTGCTGC CCGANNACCA CTACCTGANC 781 TACCAGTCCG CCCTGTTCAA AGACCCCAAC GANAANCGCG NTCACATGGT CCTGCTGGAN 841 TNCCTGANCG CCGCCGGGAT CACTGAANGN ATGAANNGAN CTGNANNAAN GANCTCNGNN 901 GGANGNNNTN NNNNNNNNGT GGCTCTNGNG GNGNNNNATC GNTCNNNATG GNNANCNNGN 961 TNNANNNNNN NNNN //
Primer: f1ori_R3 Region: BGH p(A), insert 3' Sequence file: RDB18222_B0Ffb.seq
>D07201A1_B0Ef_1_f1ori_R3_B05_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TCTTTCCGCC TCNNAANCCN TAGAGCCCAC 61 CGCATCCCCA GCATGCCTGC TATTGTCTTC CCAATCCTCC CCCTTGCTGT CCTGCCCCAC 121 CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC GATGCAATTT CCTCATTTTA 181 TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG GAAGGCACGG GGGAGGGGCA 241 AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT GATCAGCGAG CTCTAGCATT 301 TAGGTGACAC TATAGAATAG GGCCCTCTAG ATGCATGCTC GAGCGGCCGC CAGTGTGATG 361 GATATCTGCA GAATTCGCTA GCTCACTTGG CCTGGCTGGG CAGCATGGAG GCCCTGGCCA 421 CGGCGTTCTC GCACAGCTTC ACCTTGTTGT AGTCCTTGTC GTGCTCCAGG ATCTCGATCC 481 TGTGGTCGAT CTTGTGGGCG TCGGGCAGGC TCACCTCCTT CTTGGCCTTG TAGGTGGTCT 541 TGACGTCGCA CCTGTAGTGG CCGCCGCCCT CCAGCAGCAG CCTCATGTTC ACGTCGCCCT 601 TCAGCACGCC GTCCTCCACG TACATCTTCT CGGTGTCGGG CTCCCACTTC AGGGTCTTCT 661 TCTGCATCAC GGGGCCGTTG GGGGGGAAGT TGGTGCCGTC GAACCTGATG TCGTANAANA 721 ANCAGTCGCC CCTCATGCTG ATGTTGCTGG TGGCGGTGCA GATGCCCTGG TCCTCGTANG 781 TCATGCTCCT CTCCCAGTGG TAGCCCTCGG GGAANGNCTG CTTGAAGTAN TCCTGGATGT 841 CGGCGGGGNN NTTGGNGAAN GNCNNGTTGC CGTACTGGAN CNANGNGGAN AGGATGTCGN 901 NNNNNAANGG CAGGGGGGCN NCNTCNGNCA CGNTTCNNNT NCNNGNNCTG GGNGNCNNNN 961 NNNGGGNTNG CCCTNNCCNN CNNCCNNNNN NCACNNANNN TNNNNNNNNN N //
Primer: mVenus-M_F Region: insert mid Sequence file: RDB18222_B0Ffc.seq
>D07201A1_B0Ff_1_mVenus-M_F_C01_07_ABI24.ab1 1 NNNNNNNNNN NNANNNANGN NNTCNNGGCC NACTTCAAGA NCCGCCNCAA CATCGAGGAC 61 GGCGGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG 121 CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCGCCC TGTTCAAAGA CCCCAACGAG 181 AAGCGCGATC ACATGGTCCT GCTGGAGTTC CTGACCGCCG CCGGGATCAC TGAAGGCATG 241 AACGAGCTGT ACAAGGAGCT CGGTGGAGGC GGTTCAGGCG GAGGTGGCTC TGGCGGTGGC 301 GGATCGCTCG AGATGGTGAG CGTGATCAAG CCCGAGATGA AGATCAAGCT GTGCATGAGG 361 GGCACCGTGA ACGGCCACAA CTTCGTGATT GAGGGCGAGG GCAAGGGCAA CCCCTACGAG 421 GGCACCCAGA TCCTGGACCT GAACGTGACC GAGGGCGCCC CCCTGCCCTT CGCCTACGAC 481 ATCCTGTCCA CCTCGTTCCA GTACGGCAAC AGGGCCTTCA CCAAGTACCC CGCCGACATC 541 CAGGACTACT TCAAGCAGGC CTTCCCCGAG GGCTACCACT GGGAGAGGAG CATGACCTAC 601 GAGGACCAGG GCATCTGCAC CGCCACCAGC AACATCAGCA TGAGGGGCGA CTGCTTCTTC 661 TACGACATCA GGTTCGACGG CACCNACTTC CCCCCCAACG GCCCCGTGAT GCAGAAGAAG 721 ANCCTGAAGT GGGAGCCCGA CACCGANAAG ATGTACGTGG AGGANGGCGT GCTGAAGGGC 781 GACGTGAACA TGAGGCTGCT GCTGGANGGC GGCGGCCACT ACAGGTGCNA CGTCANNACC 841 ACCTACNNNN CCNAGAANGA NGTGAGCCTG CCCNANNNCC ACAAGATCGA CCACAGGATN 901 NANATNCTGG ANCACGANNN NGNNTANNNC NAGGNGANNC TGNNCNANAA CNCCGNNNNN 961 NNGGGNNTNN NNGCNNCCNN NCCNGNNNNA NNNNNNCNNN NNNNNTNNNN NNNANNNNNN 1021 NNNN //
Primer: SV40pro_ori_F Region: SV40pro_ori, NeoR Sequence file: RDB18222_B0Ffd.seq
>D07201A1_B0Ff_1_SV40pro_ori_D01_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG NNNNNNTCTG CCTCTGANCT ATTCNNGAAG TAGTGAGGAG 61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG 121 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 181 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 241 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 301 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 361 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 421 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 481 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 541 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 601 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 661 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA NGNTCTCGTC GTGACCCATG 721 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 781 GTGGCCGGCT GGGTGTGGCG GANCGCTATC ANGACATANC GTTGGCTACC CGNGATATTG 841 CTGAAGANCT NGGCGGCNAA NGGGCTGANC GCTTCCNCNN GCTTTACNGT ATCGCCNCTC 901 CNGANNNNCA NCNNANCGCC TTCNNTCNNC NNNNNNNNNA NNNNNNCNNA NCNGGNNTCT 961 GGGGGNTNCN NANNNNNNNN NN //

SRAI_pcDNA3

Distribution information

References and tips

Sequence information

References