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GEPRA-B/ pT2KXIGdeltain (#RDB15252)

Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish.

Clone info. Expression vector of genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-beta. For generation of transgenic zebrafish.
Vector backbone pT2KXIG deltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC
Gene/insert name mouse RARbeta cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S. et al., Nature 496 (7445): 363-366 (2013).
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S. et al., Nature 496 (7445): 363-366 (2013).
付加的使用条件：
Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15252 GEPRA-B/ pT2KXIGdeltain DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The GEPRA-B/ pT2KXIGdeltain was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15252).

Reference section:

Shimozono, S., Iimura, T., Kitaguchi, T., Higashijima, S., Miyawaki, A., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15252_A7H9p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F2
Sequence file: RDB15252_A7H9a.seq check
>D04498G1-2_A7H9_EF1a-C_F2_C10_07_ABI24.ab1
    1 GATGGTGGTT AGGCGGTTCG CTCTCTTCCT CACCGGGTCT GCGGCGAGTT CTAGCTGAAG
   61 CTTCCTGCAG GTCGACCGAT CCTGAGAACT TCAGGGTGAG TTTGGGGACC CTTGATTGTT
  121 CTTTCTTTTT CGCTATTGTA AAATTCATGT TATATGGAGG GGGCAAAGTT TTCAGGGTGT
  181 TGTTTAGAAT GGGAAGATGT CCCTTGTATC ACCATGGACC CTCATGATAA TTTTGTTTCT
  241 TTCACTTTCT ACTCTGTTGA CAACCATTGT CTCCTCTTAT TTTCTTTTCA TTTTCTGTAA
  301 CTTTTTCGTT AAACTTTAGC TTGCATTTGT AACGAATTTT TAAATTCACT TTTGTTTATT
  361 TGTCAGATTG TAAGTACTTT CTCTAATCAC TTTTTTTTCA AGGCAATCAG GGTATATTAT
  421 ATTGTACTTC AGCACAGTTT TAGAGAACAA TTGTTATAAT TAAATGATAA GGTAGAATAT
  481 TTCTGCATAT AAATTCTGGC TGGCGTGGAA ATATTCTTAT TGGTAGAAAC AACTACACCC
  541 TGGTCATCAT CCTGCCTTTC TCTTTATGGT TACAATGATA TACACTGTTT GAGATGAGGA
  601 TAAAATACTC TGAGTCCAAA CCGGGCCCCT CTGCTAACCA TGTTCATGCC TTCTTCTCTT
  661 TCCTACAGCT CCTGGGCAAC GTGCTGGTTG TTGTGCTGTC TCATCATTTT GGCAAAGAAT
  721 TCCTCGACGG ATCCCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
  781 CTGGTCGAGC TGCAGGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG
  841 GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
  901 GTGCCCTGGC CCACCCTCGT GACCACCCTG AGCTGGGGCG TGCAGTGCTT CAGCCCGCTA
  961 CCCCGACCAC ATGAAGCAGC ACGACTTTCT TCAAGTCCGT CATGCCCGAA TCTACGTCCA
 1021 GGAGCGCACC AATCCTTCCT CCATGAACGA CGGCACTACA AGAACCGCCG CGAGTGAGTT
1081 TCGGAGGCGA CTCCTGGTGA CGCAATCGAG CCTGAAGGAC CATCGAACCT TTCAG
//
Primer: pAxCALNL_F1
Sequence file: RDB15252_A7H9b.seq check
>D04498G1_2_A7H9_pAxCALNLF1_A01_01_ABI08.ab1
    1 CATTAAGTAT CTTATCATGT CTGGATCATC ATCGATGAAT TAATTCGAGC TCCACCGCGG
   61 TGGCGGCCGC TCTAGAGGCT CGAGTTACTT GTACAGCTCG TTCATGCCTT CAGTGATCCC
  121 GGCGGCGGTC AGGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGAA
  181 CAGGGCGGAC TGGTAGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT
  241 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCCG CCGTCCTCGA TGTTGTGGCG
  301 GATCTTGAAG TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG
  361 GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT
  421 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT
  481 CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT
  541 GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGGCGA AGCACTGCAG
  601 GCCGTAGCCC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA
  661 GAGCAGCTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT
  721 GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA
  781 CAGCTCCTCG CCCTTGCTCA CCATGAGCTC TTCATGTCCT TCAGAATTCT CCAGCATTTC
  841 CTGAATGAGA GGTGGCATTG ATCCAGGAAT TTCCATTTTC AAGGTAATTA CACGTTCGGC
  901 ACCTTTCGCG CTGATGCTGC GGAGATCTGT GATTTTCATT AAGATCTTTG GAAACATGTG
  961 AGGCTTGCTG GGTCGTCGTT TTCTAATGTA ATCTTTAGTG CTTCCAGCAG TGGTTCTTGG
 1021 AGCTTGTCTA CTTTTGTTGG GTTCCTCAAG GTCCTGGCGG TCTCCACAGA TTAAACAGGA
 1081 TGGCACTGAG GAGGCTGGTT CTGTGTCAAT CAATTTCCAG GCAAGAGCTT GGATGGCCAA
1141 GTTGAACACA AAGGTCAAGT TCAGAGAGGA ACGGA
//
Primer: RARbeta_LBD
Sequence file: RDB15252_A7H9c.seq check
>D04498G1_2_A7H9_RARbeta_LBD_C01_03_ABI08.ab1
    1 CGTAATGAAG GGGTGGGCAT TGATCCAGGA TTTCCATTTT CAAGGTAATT ACACGTTCGG
   61 CACCTTTCGC GCTGATGCTG CGGAGATCTG TGATTTTCAT TAAGATCTTT GGAAACATGT
  121 GAGGCTTGCT GGGTCGTCGT TTTCTAATGT AAATCTTTAG TGCTTCCAGC AGTGGTTCTT
  181 GGAGCTTGTC TACTTTTGTT GGTTCCTCAA GGTCCTGGCG GTCTCCACAG ATTAAACAGA
  241 TGGCACTGAG AAGGCCTGTT TCTGTGTCAT CCATTTCCAA AGGCAGGAGC TGGTTGGCAA
  301 AGGTGAACAC AAGGTCAGTC AGAGGACCGA AGCCAGCATT GTGCATCTGA GTTCGATTTA
  361 GTGTAAGGCC ATCAGAGAAA GTCATGGTGT CTTGCTCTGG GGTATACCTG GTACAAATTC
  421 TGAGAATCAA GATATCCAAG CAGGCGGCTT TGAGCAGGGT GATCTGGTCT GCGATGGTCA
  481 GACCTGTGAA GCCCGGCAGA CGCTTGGCGA ACTCCACGAT CTTAATAATG CACTTGGTGG
  541 CCAGCTCACT GAATTTGTCC CAGAGGCCCA AGTCCAATCG GACCCGGTGG TCAGCGCTGG
  601 AATTCGTGGT GTATTTACCC AGCTGGCAGA GTGAGGGAAA GGTTTCCTGG TGGGCTTTCC
  661 GGATCTTCTC AGTGAGGTCG TCTAGCTCCG CTGTCATCTC ATAGCTCTCA TGCATGCGGG
  721 CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGCTCA
  781 GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGGTT CACGGGGCCG TCGCCGATGG
  841 GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCACGATG TTGTGGCGGA
  901 TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC GGTGATATAG ACGTTGTGGC
  961 TGATGTAGTT GTACTCCAGC TTTGTGCCCC AGGATGTTGC CGTCCTCCCT TGAAAGTCGA
 1021 TGCCCTTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCTC GAACTTTCAC TCCGGCGCGG
 1081 GTTCTTGTAG TTGCCGTCGT CCATGAGAGG ATGGATGCGC TTCCTGGACG GTAGGCCTTT
1141 CGGGGCCAAG TGGAACCG
//
Primer: EF1a-N_R2
Sequence file: RDB15252_A7H9d.seq check
>D04498G1-2_A7H9_EF1a-N_R2_E10_13_ABI24.ab1
    1 GGACGGGGCA AAGGAATTGC AAGTTTGATT TGCATTGGAA AGGGTCGCTG GCTTTTGTGT
   61 TACACGCCCC TTATTTGTGC TTGATTAGAT GATCCCCCTG CTCGAGGGCC CATCTGGCCT
  121 GTGTTTCAGA CACCAGGGAG TCTCTGCTCA CGTTTCCTGC TATTTGCAGC CTCTCTATCA
  181 AGACTAATAC ACCTCTTCCC GCATCGGCTG CCTGTGAGAG GCTTTTCAGC ACTGCAGGAT
  241 TGCTTTTCAG CCCCAAAAGA GCTAGGCTTG ACACTAACAA TTTTGAGAAT CAGCTTCTAC
  301 TGAAGTTAAA TCTGAGGTTT TACAACTTTG AGTAGCGTGT ACTGGCATTA GATTGTCTGT
  361 CTTATAGTTT GATAATTAAA TACAAACAGT TCTAAAGCAG GATAAAACCT TGTATGCATT
  421 TCATTTAATG TTTTTTGAGA TTAAAAGCTT AAACAAGAAT CTCTAGTTTT CTTTCTTGCT
  481 TTTACTTTTA CTTCCTTAAT ACTCAAGTAC AATTTTAATG GAGTACTTTT TTACTTTTAC
  541 TCAAGTAAGA TTCTAGCCAG ATACTTTTAC TTTTAATTGA GTAAAATTTT CCCTAAGTAC
  601 TTGTACTTTC ACTTGAGTAA AATTTTTGAG TACTTTTTAC ACCTCTGTCA AGAACTCCTG
  661 GACAAACCTC TGACCTGTGT GGAACAGAGT GGATATGGGT GTCTGAACAG ATATTCACGT
  721 CTTTTGCAGA TCAGAGGGCA TTTCTGGTGT TGTGCAATGA CCTGGGTCCA ACAGGTAGGC
  781 GTACTCGTAT CCAAGAGCAT TGTTGGACAG GAGGTAGTCT CCATTCCTAT AAAGAGGAAG
  841 GAATGGCACC ATGTAGTAGC CGTCATTGTG GCCAATTGGT GCATTGGTAC GTGGGTAGAT
  901 GGACCGGGGA GGCTGATGAG TTCTTAGCCA GCGCTCAAGA TGCTAGAAAT GATGTAAGAA
  961 ACAATATGCA AGATGAAAGT TTAAGATAAG ATAAAAAAAT TTAACAAATA AATGCAAATT
 1021 TATGTTTTGT GTTCTATTCC ACCAATAATA AACGTCAACG CAGTAAGCCA CCAGAATATT
1081 TTTTGGTCGT TGGCTTCTGC CAAGGCAAAC CGAGACCTCC TTAAGCA
//
Primer: pT2KXIG#1
Sequence file: RDB15252_A7H9e.seq check
>D04498G1_2_A7H9_pT2KXIG#1_B01_02_ABI08.ab1
    1 TCGGGCAAAT TAACCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG
   61 CTCTAGAACT AGTGGATCTG CTGGGCTTGC TGAAGGTAGG GGGTCAAGAA CCAGAGGTGT
  121 AAAGTACTTG AGTAATTTTA CTTGATTACT GTACTTAAGT ATTATTTTTG GGGATTTTTA
  181 CTTTACTTGA GTACAATTAA AAATCAATAC TTTTACTTTT ACTTAATTAC ATTTTTTTAG
  241 AAAAAAAAGT ACTTTTTACT CCTTACAATT TTATTTACAG TCAAAAAGTA CTTATTTTTT
  301 GGAGATCACT TCATTCTATT TTCCCTTGCT ATTACCAAAC CAATTGAATT GCGCTGATGC
  361 CCAGTTTAAT TTAAATGTTA TTTATTCTGC CTATGAAAAT CGTTTTCACA TTATATGAAA
  421 TTGGTCAGAC ATGTTCATTG GTCCTTTGGA AGTGACGTCA TGTCACATCT ATTACCACAA
  481 TGCACAGCAC CTTGACCTGG AAATTAGGGA AATTATAACA GTCAATCAGT GGAAGAAAAT
  541 GGAGGAAGTA TGTGATTCAT CAGCAGCTGC GAGCAGCACA GTCCAAAATC AGCCACAGGA
  601 TCAAGAGCAC CCGTGGCCGT ATCTTCGCAG ATCTGATCTA GAGGATCATA ATCAGCCATA
  661 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
  721 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
  781 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
  841 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCATCATC GATGAATTAA
  901 TTCGAGCTCC ACCGCGGTGG CGGCCGCTCT AGAGGCTCGA GTTACTTGTA CAGCTCGTTC
  961 ATGCCTTCAG TGATCCCGGC GGCGGTCAGG AACTCCAGCA GGACCATGTG ATCGCGCTTT
 1021 CTCGTTGGGG GTCTTTTGAA CAGGGCGGGA CTGGGTAGCT CAAGTAGTGG CTTGTCGGGG
 1081 CAGCAGCACG GGGTCGTCGC GATGGGGGGT GTTCCTGCTG TAGTGATCGC GAGCTGCACG
1141 CCGGTCGGTT CCTTCGAATG TTGTGTTGGT CG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. [link to RRC of NBRP]
review Schilling, T.F., Visualizing retinoic acid morphogen gradients. Methods Cell Biol. 133: 139-163 (2016). PMID 27263412.