RDB18685 - pAAV2-SYN-tTAad-BGHpA

AAV2 expression vector of tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter, human synapsin I.

Distribution information

Sequence (full)	RDB18685zzk01.seq provided by the depositor

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Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sohn, J. et al., PLoS One, 12 (1): e0169611, 2017. Hioki, H. et al., Neurosci. Res., 63 (2): 149-154, 2009. Katayama et al. (2020). Cell 181, 1176–1187. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sohn, J. et al., PLoS One, 12 (1): e0169611, 2017. Hioki, H. et al., Neurosci. Res., 63 (2): 149-154, 2009. Katayama et al. (2020). Cell 181, 1176–1187. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
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QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18685	pAAV2-SYN-tTAad-BGHpA	DNA solution

Featured content	Expression regulation (English text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: tTA-Ad R (Pr0542) Region: tTA-Advanced 5', h synapsin pro, attB2, AAV2 L-ITR Sequence file: RDB18685_B1B5a.seq
>D07612A2_B1B5_1_tTA-Ad R_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NNNCTCTTGT CCNGTCNAGA NATGGTGAAT 61 TCGATATCAA GCTTATCGAT ACCGTCCGCC GCAGCGCAGA TGGTCGCGCC CGTGCCCCCC 121 TATCTCGCGC CTCGCGTGGT GCGGTCCGGC TGGGCCGGCG GCGGCGCGGA CGCGACCAAG 181 GTGGCCGGGA AGGGGAGTTT GCGGGGGACC GGCGAGTGAC GTCAGCGCGC CTTCAGTGCT 241 GAGGCGGCGG TGGCGCGCGC CGCCAGGCGG GGGCGAAGGC ACTGTCCGCG GTGCTGAAGC 301 TGGCAGTGCG CACGCGCCTC GCCGCATCCT GTTTCCCCTC CCCCTCTCTG ATAGGGGATG 361 CGCAATTTGG GGAATGGGGG TTGGGTGCTT GTCCAGTGGG TCGGGGTCGG TCGTCAGGTA 421 GGCACCCCCA CCCCGCCTCA TCCTGGTCCT AAAACCCACT TGCACTCATA CGCAGGGCCC 481 TCTGCAGGAC CTCGAGATAT CTAGACCCAG CTTTCTTGTA CAAAGTGGTA CGCGTGCGGC 541 CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTN //
Primer: BGHpA_F (Pr0373) Region: bGH pA, attB1, AAV2R-ITR Sequence file: RDB18685_B1B5b.seq
>D07612A2_B1B5_1_BGHpA_F_H05_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGGG GCANGANAGC AAGGGGGAGG 61 NNTGGGAAGA NAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGGC GGCCGCGAAT 121 TCGGTACCGG ATCCAGTCGA CTGAATTGGT TCCTTTAAAG CCTGCTTTTT TGTACAAACT 181 TGTCACGTGC GGACCGAGCG GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT 241 CTGCGCGN //
Primer: attB2-R1 (Pr0411) Region: h synapsin pro, tTA-Advanced 5' Sequence file: RDB18685_B1B5c.seq
>D07612A2_B1B5_1_attB2-R1_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCG NATGAGNGNN NNTGGGTTTT AGGANCAGGA 61 TGAGGCGGGG TGGGGGTGCC TACCTGACGA CCGACCCCGA CCCACTGGAC AAGCACCCAA 121 CCCCCATTCC CCAAATTGCG CATCCCCTAT CAGAGAGGGG GAGGGGAAAC AGGATGCGGC 181 GAGGCGCGTG CGCACTGCCA GCTTCAGCAC CGCGGACAGT GCCTTCGCCC CCGCCTGGCG 241 GCGCGCGCCA CCGCCGCCTC AGCACTGAAG GCGCGCTGAC GTCACTCGCC GGTCCCCCGC 301 AAACTCCCCT TCCCGGCCAC CTTGGTCGCG TCCGCGCCGC CGCCGGCCCA GCCGGACCGC 361 ACCACGCGAG GCGCGAGATA GGGGGGCACG GGCGCGACCA TCTGCGCTGC GGCGGACGGT 421 ATCGATAAGC TTGATATCGA ATTCACCATG TCTAGACTGG ACAAGAGCAA AGTCATAAAC 481 TCTGCTCTGG AATTACTCAA TGAAGTCGGT ATCGAAGGCC TGACGACAAG GAAACTCGCT 541 CAAAAGCTGG GAGTTGAGCA GCCTACCCTG TACTGGCACG TGAAGAACAA GCGGGCCCTG 601 CTCGATGCCC TGGCAATCGA GATGCTGGAC AGGCATCATA CCCACTTCTG CCCCCTGGAA 661 GGCGAGTCAT GGCAAGACTT TCTGCGGAAC AACGCCAAGT CATTCCGCTG TGCTCTCCTC 721 TCACATCGCG ACGGGGCTAA AGTGCATCTC NGCACCCGCC CAACAGAGAA ACAGTACGAA 781 ACCCTGGAAA ATCAGCTCGC GTTCCTGTGT CAGCNANGCT TCTCCCTGGA GAACGCACTG 841 TACGCTCTGT CCGCCGTGNG CCACTTTACA CTGGGCTGCG NATTGNAGGA TCNGGANCNT 901 CAAGTANNNA AAGANNNAAG ANAGANNNCT NNNNNNGANT NCNATGCCCC CACTTCTGAG 961 ANANGCNANT NGAGCTGNNC GANNNNNNNN GNNNCCNNNN NNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA, tTA-Advanced 3' Sequence file: RDB18685_B1B5d.seq
>D07612A2_B1B5_1_bGH_rev3_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGGCANNNNN NNNNNGCTGG CAACTAGAAG 61 GCACAGACTA GTGGATCCTT ACTTAGTTAC CCGGGGAGCA TGTCAAGGTC AAAATCGTCA 121 AGAGCGTCAG CAGGCAGCAT ATCAAGGTCA AAGTCGTCAA GGGCATCGGC TGGGAGCATG 181 TCTAAGTCAA AATCGTCAAG GGCGTCGGCC GGCCCGCCGC TTTCGCACTT TAGCTGTTTC 241 TCCAGGCCAC ATATGATTAG TTCCAGGCCG AAAAGGAAGG CAGGTTCGGC TCCCTGATGG 301 TCGAACAGCT CAATTGCTTG TCTCAGAAGT GGGGGCATAG AATCGGTGGT AGGTGTCTCT 361 CTTTCCTCTT TTGCTACTTG ATGCTCCTGA TCCTCCAATA CGCAGCCCAG TGTAAAGTGG 421 CCCACGGCGG ACAGAGCGTA CAGTGCGTTC TCCAGGGAGA AGCCTTGCTG ACACAGGAAC 481 GCGAGCTGAT TTTCCAGGGT TTCGTACTGT TTCTCTGTTG GGCGGGTGCC GAGATGCACT 541 TTAGCCCCGT CGCGATGTGA GAGGAGAGCA CAGCGGAATG ACTTGGCGTT GTTCCGCAGA 601 AAGTCTTGCC ATGACTCGCC TTCCAGGGGG CAGAAGTGGG TATGATGCCT GTCCAGCATC 661 TCGATTGCCA GGGCATCGAG CAGGGCCCGC TTGTTCTTCA CGTGCCAGTA CAGGGTAGGC 721 TGCTCAACTC CCAGCTTTTG AGCGAGTTTC CTTGTCGTCA GGCCTTCGAT ACCGACTTCA 781 TTGAGTAATT CCNGANCANA NTTTATGACT TTGCTCTTGT CCAGTCTAGA CATGGTGAAT 841 TCNATATCAA GCTTATCGAT NCCGNCCGNN NCANCGCANA NGGTCGCGCC CGTGCCCCCC 901 TATCNCGCGC CTCGCGTGGT GNNGNNCNGG NTGGNNCNGC GNNGGCGCGG ANNNNANCAA 961 GGNGNCCNGG AAGGGGANTT TGCGGGGGNA NNNNCNANNG NNGTCAGCNC NNCTNCANNG 1021 NTNNNNNNNN CNNNNGNNCN NNNNNNNCNN NNNNNNNN //