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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV2-SYN-tTAad-BGHpA

AAV2 expression vector of tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter, human synapsin I.

Catalog number RDB18685
Resource name pAAV2-SYN-tTAad-BGHpA
Clone info. AAV2 expression vector of second generation tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter (human synapsin I (SYN)). The ITRs (inverted terminal repeats) are derived from AAV serotype 2.
Expression was confirmed by the depositor, co-transfection to cultured cell with a plasmid vector that expresses fluorescent protein(s) under TRE promoter.
Vector backbone plasmid
Selectable markers Ampicillin
Depositor|Developer Miyawaki, Atsushi | Hioki, Hiroyuki | Katayama, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sohn, J. et al., PLoS One, 12 (1): e0169611, 2017. Hioki, H. et al., Neurosci. Res., 63 (2): 149-154, 2009. Katayama et al. (2020). Cell 181, 1176–1187.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18685 pAAV2-SYN-tTAad-BGHpA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18685_B1B5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: tTA-Ad R
Region: tTA-Advanced 5', h synapsin pro, attB2, AAV2 L-ITR
Sequence file: RDB18685_B1B5a.seq check
>D07612A2_B1B5_1_tTA-Ad R_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NNNCTCTTGT CCNGTCNAGA NATGGTGAAT
   61 TCGATATCAA GCTTATCGAT ACCGTCCGCC GCAGCGCAGA TGGTCGCGCC CGTGCCCCCC
  121 TATCTCGCGC CTCGCGTGGT GCGGTCCGGC TGGGCCGGCG GCGGCGCGGA CGCGACCAAG
  181 GTGGCCGGGA AGGGGAGTTT GCGGGGGACC GGCGAGTGAC GTCAGCGCGC CTTCAGTGCT
  241 GAGGCGGCGG TGGCGCGCGC CGCCAGGCGG GGGCGAAGGC ACTGTCCGCG GTGCTGAAGC
  301 TGGCAGTGCG CACGCGCCTC GCCGCATCCT GTTTCCCCTC CCCCTCTCTG ATAGGGGATG
  361 CGCAATTTGG GGAATGGGGG TTGGGTGCTT GTCCAGTGGG TCGGGGTCGG TCGTCAGGTA
  421 GGCACCCCCA CCCCGCCTCA TCCTGGTCCT AAAACCCACT TGCACTCATA CGCAGGGCCC
  481 TCTGCAGGAC CTCGAGATAT CTAGACCCAG CTTTCTTGTA CAAAGTGGTA CGCGTGCGGC
541 CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTN
//
Primer: BGHpA_F
Region: bGH pA, attB1, AAV2R-ITR
Sequence file: RDB18685_B1B5b.seq check
>D07612A2_B1B5_1_BGHpA_F_H05_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGGG GCANGANAGC AAGGGGGAGG
   61 NNTGGGAAGA NAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGGC GGCCGCGAAT
  121 TCGGTACCGG ATCCAGTCGA CTGAATTGGT TCCTTTAAAG CCTGCTTTTT TGTACAAACT
  181 TGTCACGTGC GGACCGAGCG GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT
241 CTGCGCGN
//
Primer: attB2-R1
Region: h synapsin pro, tTA-Advanced 5'
Sequence file: RDB18685_B1B5c.seq check
>D07612A2_B1B5_1_attB2-R1_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCG NATGAGNGNN NNTGGGTTTT AGGANCAGGA
   61 TGAGGCGGGG TGGGGGTGCC TACCTGACGA CCGACCCCGA CCCACTGGAC AAGCACCCAA
  121 CCCCCATTCC CCAAATTGCG CATCCCCTAT CAGAGAGGGG GAGGGGAAAC AGGATGCGGC
  181 GAGGCGCGTG CGCACTGCCA GCTTCAGCAC CGCGGACAGT GCCTTCGCCC CCGCCTGGCG
  241 GCGCGCGCCA CCGCCGCCTC AGCACTGAAG GCGCGCTGAC GTCACTCGCC GGTCCCCCGC
  301 AAACTCCCCT TCCCGGCCAC CTTGGTCGCG TCCGCGCCGC CGCCGGCCCA GCCGGACCGC
  361 ACCACGCGAG GCGCGAGATA GGGGGGCACG GGCGCGACCA TCTGCGCTGC GGCGGACGGT
  421 ATCGATAAGC TTGATATCGA ATTCACCATG TCTAGACTGG ACAAGAGCAA AGTCATAAAC
  481 TCTGCTCTGG AATTACTCAA TGAAGTCGGT ATCGAAGGCC TGACGACAAG GAAACTCGCT
  541 CAAAAGCTGG GAGTTGAGCA GCCTACCCTG TACTGGCACG TGAAGAACAA GCGGGCCCTG
  601 CTCGATGCCC TGGCAATCGA GATGCTGGAC AGGCATCATA CCCACTTCTG CCCCCTGGAA
  661 GGCGAGTCAT GGCAAGACTT TCTGCGGAAC AACGCCAAGT CATTCCGCTG TGCTCTCCTC
  721 TCACATCGCG ACGGGGCTAA AGTGCATCTC NGCACCCGCC CAACAGAGAA ACAGTACGAA
  781 ACCCTGGAAA ATCAGCTCGC GTTCCTGTGT CAGCNANGCT TCTCCCTGGA GAACGCACTG
  841 TACGCTCTGT CCGCCGTGNG CCACTTTACA CTGGGCTGCG NATTGNAGGA TCNGGANCNT
  901 CAAGTANNNA AAGANNNAAG ANAGANNNCT NNNNNNGANT NCNATGCCCC CACTTCTGAG
961 ANANGCNANT NGAGCTGNNC GANNNNNNNN GNNNCCNNNN NNNNNN
//
Primer: bGH_rev3
Region: bGH pA, tTA-Advanced 3'
Sequence file: RDB18685_B1B5d.seq check
>D07612A2_B1B5_1_bGH_rev3_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGGCANNNNN NNNNNGCTGG CAACTAGAAG
   61 GCACAGACTA GTGGATCCTT ACTTAGTTAC CCGGGGAGCA TGTCAAGGTC AAAATCGTCA
  121 AGAGCGTCAG CAGGCAGCAT ATCAAGGTCA AAGTCGTCAA GGGCATCGGC TGGGAGCATG
  181 TCTAAGTCAA AATCGTCAAG GGCGTCGGCC GGCCCGCCGC TTTCGCACTT TAGCTGTTTC
  241 TCCAGGCCAC ATATGATTAG TTCCAGGCCG AAAAGGAAGG CAGGTTCGGC TCCCTGATGG
  301 TCGAACAGCT CAATTGCTTG TCTCAGAAGT GGGGGCATAG AATCGGTGGT AGGTGTCTCT
  361 CTTTCCTCTT TTGCTACTTG ATGCTCCTGA TCCTCCAATA CGCAGCCCAG TGTAAAGTGG
  421 CCCACGGCGG ACAGAGCGTA CAGTGCGTTC TCCAGGGAGA AGCCTTGCTG ACACAGGAAC
  481 GCGAGCTGAT TTTCCAGGGT TTCGTACTGT TTCTCTGTTG GGCGGGTGCC GAGATGCACT
  541 TTAGCCCCGT CGCGATGTGA GAGGAGAGCA CAGCGGAATG ACTTGGCGTT GTTCCGCAGA
  601 AAGTCTTGCC ATGACTCGCC TTCCAGGGGG CAGAAGTGGG TATGATGCCT GTCCAGCATC
  661 TCGATTGCCA GGGCATCGAG CAGGGCCCGC TTGTTCTTCA CGTGCCAGTA CAGGGTAGGC
  721 TGCTCAACTC CCAGCTTTTG AGCGAGTTTC CTTGTCGTCA GGCCTTCGAT ACCGACTTCA
  781 TTGAGTAATT CCNGANCANA NTTTATGACT TTGCTCTTGT CCAGTCTAGA CATGGTGAAT
  841 TCNATATCAA GCTTATCGAT NCCGNCCGNN NCANCGCANA NGGTCGCGCC CGTGCCCCCC
  901 TATCNCGCGC CTCGCGTGGT GNNGNNCNGG NTGGNNCNGC GNNGGCGCGG ANNNNANCAA
  961 GGNGNCCNGG AAGGGGANTT TGCGGGGGNA NNNNCNANNG NNGTCAGCNC NNCTNCANNG
1021 NTNNNNNNNN CNNNNGNNCN NNNNNNNCNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660.

2021.04.05

GNP_filter3_RDBDEP_html_210401.pl