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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV2-SYN-tTAad-BGHpA

AAV2 expression vector of tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter, human synapsin I.

Catalog number RDB18685
Resource name pAAV2-SYN-tTAad-BGHpA
Clone info. AAV2 expression vector of second generation tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter (human synapsin I (SYN)). The ITRs (inverted terminal repeats) are derived from AAV serotype 2.
Comment Expression was confirmed by the depositor, co-transfection to cultured cell with a plasmid vector that expresses fluorescent protein(s) under TRE promoter.
Vector backbone plasmid
Selectable markers Ampicillin
Depositor|Developer Miyawaki, Atsushi | Hioki, Hiroyuki | Katayama, Hiroyuki |
Other clones in our bank

External Database

            Reference sequence
              
             
            Sequence (full) RDB18685zzk01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Sohn, J. et al., PLoS One, 12 (1): e0169611, 2017. Hioki, H. et al., Neurosci. Res., 63 (2): 149-154, 2009. Katayama et al. (2020). Cell 181, 1176–1187.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks Remember that you will be working with samples containing infectious virus.
            Please obtain written approval of Dr. Atsushi Miyawaki.
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            提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Sohn, J. et al., PLoS One, 12 (1): e0169611, 2017. Hioki, H. et al., Neurosci. Res., 63 (2): 149-154, 2009. Katayama et al. (2020). Cell 181, 1176–1187.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18685 pAAV2-SYN-tTAad-BGHpA DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660.

            Further references such as user reports and related articles (go to bottom)

            Featured content


            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18685_B1B5p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: tTA-Ad R
            Region: tTA-Advanced 5', h synapsin pro, attB2, AAV2 L-ITR
            Sequence file: RDB18685_B1B5a.seq check
            >D07612A2_B1B5_1_tTA-Ad R_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NNNCTCTTGT CCNGTCNAGA NATGGTGAAT
               61 TCGATATCAA GCTTATCGAT ACCGTCCGCC GCAGCGCAGA TGGTCGCGCC CGTGCCCCCC
              121 TATCTCGCGC CTCGCGTGGT GCGGTCCGGC TGGGCCGGCG GCGGCGCGGA CGCGACCAAG
              181 GTGGCCGGGA AGGGGAGTTT GCGGGGGACC GGCGAGTGAC GTCAGCGCGC CTTCAGTGCT
              241 GAGGCGGCGG TGGCGCGCGC CGCCAGGCGG GGGCGAAGGC ACTGTCCGCG GTGCTGAAGC
              301 TGGCAGTGCG CACGCGCCTC GCCGCATCCT GTTTCCCCTC CCCCTCTCTG ATAGGGGATG
              361 CGCAATTTGG GGAATGGGGG TTGGGTGCTT GTCCAGTGGG TCGGGGTCGG TCGTCAGGTA
              421 GGCACCCCCA CCCCGCCTCA TCCTGGTCCT AAAACCCACT TGCACTCATA CGCAGGGCCC
              481 TCTGCAGGAC CTCGAGATAT CTAGACCCAG CTTTCTTGTA CAAAGTGGTA CGCGTGCGGC
            541 CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTN
            //
            Primer: BGHpA_F
            Region: bGH pA, attB1, AAV2R-ITR
            Sequence file: RDB18685_B1B5b.seq check
            >D07612A2_B1B5_1_BGHpA_F_H05_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGGG GCANGANAGC AAGGGGGAGG
               61 NNTGGGAAGA NAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGGC GGCCGCGAAT
              121 TCGGTACCGG ATCCAGTCGA CTGAATTGGT TCCTTTAAAG CCTGCTTTTT TGTACAAACT
              181 TGTCACGTGC GGACCGAGCG GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT
            241 CTGCGCGN
            //
            Primer: attB2-R1
            Region: h synapsin pro, tTA-Advanced 5'
            Sequence file: RDB18685_B1B5c.seq check
            >D07612A2_B1B5_1_attB2-R1_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCG NATGAGNGNN NNTGGGTTTT AGGANCAGGA
               61 TGAGGCGGGG TGGGGGTGCC TACCTGACGA CCGACCCCGA CCCACTGGAC AAGCACCCAA
              121 CCCCCATTCC CCAAATTGCG CATCCCCTAT CAGAGAGGGG GAGGGGAAAC AGGATGCGGC
              181 GAGGCGCGTG CGCACTGCCA GCTTCAGCAC CGCGGACAGT GCCTTCGCCC CCGCCTGGCG
              241 GCGCGCGCCA CCGCCGCCTC AGCACTGAAG GCGCGCTGAC GTCACTCGCC GGTCCCCCGC
              301 AAACTCCCCT TCCCGGCCAC CTTGGTCGCG TCCGCGCCGC CGCCGGCCCA GCCGGACCGC
              361 ACCACGCGAG GCGCGAGATA GGGGGGCACG GGCGCGACCA TCTGCGCTGC GGCGGACGGT
              421 ATCGATAAGC TTGATATCGA ATTCACCATG TCTAGACTGG ACAAGAGCAA AGTCATAAAC
              481 TCTGCTCTGG AATTACTCAA TGAAGTCGGT ATCGAAGGCC TGACGACAAG GAAACTCGCT
              541 CAAAAGCTGG GAGTTGAGCA GCCTACCCTG TACTGGCACG TGAAGAACAA GCGGGCCCTG
              601 CTCGATGCCC TGGCAATCGA GATGCTGGAC AGGCATCATA CCCACTTCTG CCCCCTGGAA
              661 GGCGAGTCAT GGCAAGACTT TCTGCGGAAC AACGCCAAGT CATTCCGCTG TGCTCTCCTC
              721 TCACATCGCG ACGGGGCTAA AGTGCATCTC NGCACCCGCC CAACAGAGAA ACAGTACGAA
              781 ACCCTGGAAA ATCAGCTCGC GTTCCTGTGT CAGCNANGCT TCTCCCTGGA GAACGCACTG
              841 TACGCTCTGT CCGCCGTGNG CCACTTTACA CTGGGCTGCG NATTGNAGGA TCNGGANCNT
              901 CAAGTANNNA AAGANNNAAG ANAGANNNCT NNNNNNGANT NCNATGCCCC CACTTCTGAG
            961 ANANGCNANT NGAGCTGNNC GANNNNNNNN GNNNCCNNNN NNNNNN
            //
            Primer: bGH_rev3
            Region: bGH pA, tTA-Advanced 3'
            Sequence file: RDB18685_B1B5d.seq check
            >D07612A2_B1B5_1_bGH_rev3_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGGCANNNNN NNNNNGCTGG CAACTAGAAG
               61 GCACAGACTA GTGGATCCTT ACTTAGTTAC CCGGGGAGCA TGTCAAGGTC AAAATCGTCA
              121 AGAGCGTCAG CAGGCAGCAT ATCAAGGTCA AAGTCGTCAA GGGCATCGGC TGGGAGCATG
              181 TCTAAGTCAA AATCGTCAAG GGCGTCGGCC GGCCCGCCGC TTTCGCACTT TAGCTGTTTC
              241 TCCAGGCCAC ATATGATTAG TTCCAGGCCG AAAAGGAAGG CAGGTTCGGC TCCCTGATGG
              301 TCGAACAGCT CAATTGCTTG TCTCAGAAGT GGGGGCATAG AATCGGTGGT AGGTGTCTCT
              361 CTTTCCTCTT TTGCTACTTG ATGCTCCTGA TCCTCCAATA CGCAGCCCAG TGTAAAGTGG
              421 CCCACGGCGG ACAGAGCGTA CAGTGCGTTC TCCAGGGAGA AGCCTTGCTG ACACAGGAAC
              481 GCGAGCTGAT TTTCCAGGGT TTCGTACTGT TTCTCTGTTG GGCGGGTGCC GAGATGCACT
              541 TTAGCCCCGT CGCGATGTGA GAGGAGAGCA CAGCGGAATG ACTTGGCGTT GTTCCGCAGA
              601 AAGTCTTGCC ATGACTCGCC TTCCAGGGGG CAGAAGTGGG TATGATGCCT GTCCAGCATC
              661 TCGATTGCCA GGGCATCGAG CAGGGCCCGC TTGTTCTTCA CGTGCCAGTA CAGGGTAGGC
              721 TGCTCAACTC CCAGCTTTTG AGCGAGTTTC CTTGTCGTCA GGCCTTCGAT ACCGACTTCA
              781 TTGAGTAATT CCNGANCANA NTTTATGACT TTGCTCTTGT CCAGTCTAGA CATGGTGAAT
              841 TCNATATCAA GCTTATCGAT NCCGNCCGNN NCANCGCANA NGGTCGCGCC CGTGCCCCCC
              901 TATCNCGCGC CTCGCGTGGT GNNGNNCNGG NTGGNNCNGC GNNGGCGCGG ANNNNANCAA
              961 GGNGNCCNGG AAGGGGANTT TGCGGGGGNA NNNNCNANNG NNGTCAGCNC NNCTNCANNG
            1021 NTNNNNNNNN CNNNNGNNCN NNNNNNNCNN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660.

            2022.07.27

            GNP_filter3_RDBDEP_html_220727.pl