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tFucci(CA)2.1/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15447
Resource name tFucci(CA)2.1/pCSII-EF
Clone info. (provision suspended)
Lentivirus expression vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Anemonia majano CFP cDNA
Reference of insert sequence LC212777 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Anemonia majano CFP

        Reference sequence
          
        Lentiviral plasmid list. [link]

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Ordering instruction.[link] 
        Order form 
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        This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        提供依頼書 [Word]
        Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
        Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15447 tFucci(CA)2.1/pCSII-EF DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15447_B1K5p1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: EF1a-C_F
        Region: EF-1 alpha pro,insert 5'(mCherry 5')
        Sequence file: RDB15447_B1K5g.seq check
        >D05018D2_B1K5_1_EF1a-C_F_A03_03_ABI24.ab1
            1 NNNNNNNNNN NNTNNNNNNN NNNNNNTTTG GNNCTTGGTT NNTTCTCNAG CCTCAGACAG
           61 TGGTTCAAAG TTTTTTTCTT CCNTTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGAAT
          121 TCTGCAGCGG CCGCGGATCC ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA
          181 TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG
          241 AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG
          301 TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG
          361 GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC
          421 CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA
          481 CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA
          541 ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG
          601 AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA
          661 AGGACGGCGG CCACTACGAC GCTGNNGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC
          721 AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT
          781 ACNCCATCGT GNAACAGTAC GAACGCGCCG ANGGCCGCCA CTCCACCGGC GGCATGGACG
          841 ANCTNNACAA GGGATATCCA TCACACNNGC GGCCGCTCGA GATGGNNCAG CGCCGCNNCA
          901 NCGANNTCTN NGCGNGCNGC CGCCCCNGNN NCNCCNNNNT CGNGNCNCCN NNNNNNGNNN
        961 TNNNNNANCC CNNNNNNNNN N
        //
        Primer: PRE_rev
        Region: PRE,insert 3'(hGem(1/110),AmCyan1 3')
        Sequence file: RDB15447_B1K5h.seq check
        >D05018D2_B1K5_1_PRE_rev_B03_06_ABI24.ab1
            1 NNNNNNNNNN NNNNNNGNAA ANGGNNCACN TAGTTAAGAN TACCAGTCAA TCTTTCNCAA
           61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
          121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT
          181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT
          241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC
          301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG
          361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT
          421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC
          481 CGAAGGGCAC CACGGAGGTG ATGTGGGCCA CGGCGTGCTC GGTCAGCTGC ACGCTGTTGC
          541 CGCCCTTGTC CAGGTCGGTT CTGGCGATGC GGTGCTCCAC CACGTGGTTG GGGGGCATGG
          601 TCACGGGCTT CTTGGTCTTG TAGGAGGTGT GGAACTGGCA TCTGTAGTTG CCGCCGCCCT
          661 GCAGCATCAG GAAGGCGGTC ACGTCGCCCT TCAAGATGCC GTCGCACACG GTCATCTTCT
          721 CGAANGAGGG GTCCCAGCCG GTGGTCTTCT TGGNCATCAC GGGGCCGTCN GCGGGGAAGT
          781 TCACGCCGTG GAAGGTGGAC TTGTGCTCGA AGCAGTTGNC CTTCNNNTGA TCTCCCAGCT
          841 GGCGGNGGCC ACGCCGCCNN CCTCGTANGT GAAGGTTCTC TCGNANGACA TGCCNNCGGG
          901 GAANGNCTGC TNGANNNAGN NNGNNNTGCT GGNGGGGNAN GNNNGNNGNN NCANCNGNNN
        961 NNNNNNN
        //
        Primer: mCherry-C_F
        Region: insert mid(mCherry 3',hCdt1(1/100)Cy(-),P2A,AmCyan1 5')
        Sequence file: RDB15447_B1K5i.seq check
        >D05018D2_B1K5_1_mCherry-C_F_C03_09_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN CTGCNNGGNG CCTACNACGT CAACATCAAG TTGGACATCA
           61 CCTCCCACAA CGNGGNNTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT
          121 CCACCGGCGG CATGGACGAG CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGA
          181 TGGAGCAGCG CCGCGTCACC GACTTCTTCG CGCGCCGCCG CCCCGGGCCC CCCCGCATCG
          241 CGCCGCCCAA GCTGGCCTGC CGCACCCCCA GCCCCGCCAG GCCCGCACTC CGCGCCCCGG
          301 CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCCGCCCCC GGACGCGACC
          361 AGGCCAGGCC ACCGGCCCGC GCGGCCGCGC GGCTGTCGGT GGACGAGGTT TCCAGCCCCA
          421 GTACCCCCGA GGCCCCAGAC ATCCCAGCCT GCCCTTCTCC GGGCCAGAAG ATAAAGAAAA
          481 CCGGTCGCGC CAAGCGCGGC AGCGGCGCCA CCAACTTCAG CCTGCTGAAG CAGGCCGGCG
          541 ACGTGGAGGA GAACCCCGGC CCCCGCGAAT TCGCCACCAT GGCCCTGTCC AACAAGTTCA
          601 TCGGCGACGA CATGAAGATG ACCTACCACA TGGACGGCTG CGTGAACGGC CACTACTTCA
          661 CCGTGAAGGG CGAGGGCAGC GGCAAGCCCT ACGAGGGCAC CCAGACCTCC ACCTTCAAGG
          721 TGACCATGGC CAACGGCGGC CCCCTGGCCT TCTCCTTCGA CATCCTGTCC ACCGTGTTCA
          781 TGTACGGCAA CCGCTGCTTC ACCGCCTACC CCACCAGCAT GCCCGACTAC TTCAAGCNNG
          841 NCTTCCCCGA CGGCATGTCC TACNAGAGAA NCTTCACCTA CNNNGNNGGC GGCGNGGNCA
          901 CCGCCAGCTG GGNNATCANC CNGNANNGGN NNCNGNNTCN NNNACNNGNN NNNCNTTNCN
        961 NNNGNNNNNN NNNTNNCNNN NCNNNNNGNN CCNNNNNNNN NN
        //
        Primer: CMV_Forward
        Region: CMV pro,5'LTR,HIV-1 psi
        Sequence file: RDB15447_B1K5j.seq check
        >D05018D2_B1K5_1_CMV_Forward_D03_12_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NTTGNNGCNN NTGGGCGGTA GGCGTGTACG GTGGGAGGTC
           61 TATATAAGCA GCGCGTTTTG CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG
          121 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT
          181 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC
          241 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA
          301 GGGAAACCAG AGGAGCTCTC TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA
          361 GGCGAGGGGC GGCGACTGGT GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG
          421 AGAGATGGGT GCGAGAGCGT CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA
          481 ATTCGGTTAA GGCCAGGGGG AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC
          541 AGGGAGCTAG AACGATTCGC AGTTAATCCT GGCCTGTTAG AAACATCAGA AGGCTGTAGA
          601 CAAATACTGG GACAGCTACA ACCATCCCTT CAGACAGGAT CAGAAGAACT TAGATCATTA
          661 TATAATACAG TAGCAACCCT CTATTGTGTG CATCAAAGGA TAGAGATAAA AGACACCAAG
          721 GAAGCTTTAG ACAAGATAGA GGAAGAGCAA AACNAAAGTA AGACCACCGC ACANCAAGGG
          781 CCGCTGATCT TCNGACCTGG NNGANGANAT ATGAGGGACA ATTNGAGAAN TGAATTATAT
          841 AAATATNAAA GTAGTAAAAA TTGANNCATT NNGANTAGCN CCNACNNAGG CAAANANAAG
          901 NNNGGNGCAG AGAGAAAAAA GANCANTNNN NNTANGNNCT TTGTTNCTTN GGNTCNNGGG
        961 NNNNNNCNNN NNNNNNN
        //
        Primer: BGH_rev3
        Region: 3'LTR,PRE
        Sequence file: RDB15447_B1K5k.seq check
        >D05018D2_B1K5_1_bGH_rev3_F03_18_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNG NNGNNGGNNN NNNACNGANG GCTGGCAACT AGAAGGCACA
           61 GTCGAGGCTG NTCNNCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA
          121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG
          181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC
          241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC
          301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC
          361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC
          421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA
          481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA
          541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC
          601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG
          661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA
          721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG
          781 ACGGTATCGA TGCGGGGNGG CGGCCCAAAG GGAGATCCGA CTCGTCTGAN GGCGAANGCG
          841 AANANGCGGA ANANGNCGCA NANCCGGCAG CNNGCCGCGG GAANGAANNN CCGCNNGNNT
          901 NNNGGNCGAA NGNNNGTANC ANANNNNNNT CCNNNGNNNN NNNCANGNGG NNNCNCNNNN
        961 NNNNNN
        //
        Primer: SV40pro_F
        Region: Zeo,SV40 pA
        Sequence file: RDB15447_B1K5l.seq check
        >D05018D2_B1K5_1_SV40pro_F_G03_21_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNANNANTGN NGNNGCTTTT TTGGNNGCCT AGGCTTTTGC
           61 AAAAAGCTCC CGGGAGCTTG NATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
          121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
          181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
          241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
          301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
          361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
          421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
          481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
          541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
          601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
          661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
          721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
          781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC NACCTCTAGC TAGAGCTTGN
          841 CGTANTCATG GNCATAGCTG TTTCCTGTGN NAAATTGTTA TCCGCTCACN ATNNCNCNCA
          901 ACATACNNNC CGGAAGCATA AAGNGNAAAG CCNNGGGGNN NNTNNNGAGN GAGCNNNNNC
        961 NNNNNNANNN NNNNTNNNNN NNNNN
        //
        Primer: mRFP1-inner1
        Region: insert mid(mCherry 3',hCdt1(1/100)Cy(-),P2A)
        Sequence file: RDB15447_B1K5m.seq check
        >D05018D2_B1K5_1_mRFP1-inner1_H03_24_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNGNNTCC TCCNTGCAGG NNGGCGNNTT CNTCTACNAG
           61 GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG
          121 GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC
          181 AAGCAGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA GACCACCTAC
          241 AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAACG TCAACATCAA GTTGGACATC
          301 ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG AACGCGCCGA GGGCCGCCAC
          361 TCCACCGGCG GCATGGACGA GCTGTACAAG GGATATCCAT CACACTGGCG GCCGCTCGAG
          421 ATGGAGCAGC GCCGCGTCAC CGACTTCTTC GCGCGCCGCC GCCCCGGGCC CCCCCGCATC
          481 GCGCCGCCCA AGCTGGCCTG CCGCACCCCC AGCCCCGCCA GGCCCGCACT CCGCGCCCCG
          541 GCCTCCGCTA CCAGTGGCAG CCGCAAGCGC GCCCGCCCGC CCGCCGCCCC CGGACGCGAC
          601 CAGGCCAGGC CACCGGCCCG CGCGGCCGCG CGGCTGTCGG TGGACGAGGT TTCCAGCCCC
          661 AGTACCCCCG AGGCCCCAGA CATCCCAGCC TGCCCTTCTC CGGGCCAGAA GATAAAGAAA
          721 ACCGGTCGCG CCAAGCGCGG CAGCGGCGCC ACCAACTTCA GCCTGCTGAA GCAGGCCGGC
          781 GNCGTGGANG ANAANNCCGG CCCCCGCGAA TTCGCCACCA TGGCCCTGTC CNACAAGTTC
          841 ATCGGCGANG ACATGAAGAN GANCNACCNC ATGGNNNGNN GCGNGAACNG NCNCNACTNN
        901 ANNNNGNANN GGNGAGGGNA GNGGNNNNNN NTACNANGGC NCCCANNNNN NNNNN
        //
        Primer: AmCyan1-H_F
        Region: insert mid(AmCyan1 3',hGem(1/110) 5')
        Sequence file: RDB15447_B1K5n.seq check
        >D05018D2_B1K5_1_AmCyan1-H_F_G02_20_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN GNNNNNNNNN CTCCNCCTNN NNNGTGNNCA TGGCCAACGG
           61 CGGCCCCCTG GNCTTCTCCT TCGACATCCT GTCCACCGTG TTCATGTACG GCAACCGCTG
          121 CTTCACCGCC TACCCCACCA GCATGCCCGA CTACTTCAAG CAGGCCTTCC CCGACGGCAT
          181 GTCCTACGAG AGAACCTTCA CCTACGAGGA CGGCGGCGTG GCCACCGCCA GCTGGGAGAT
          241 CAGCCTGAAG GGCAACTGCT TCGAGCACAA GTCCACCTTC CACGGCGTGA ACTTCCCCGC
          301 CGACGGCCCC GTGATGGCCA AGAAGACCAC CGGCTGGGAC CCCTCCTTCG AGAAGATGAC
          361 CGTGTGCGAC GGCATCTTGA AGGGCGACGT GACCGCCTTC CTGATGCTGC AGGGCGGCGG
          421 CAACTACAGA TGCCAGTTCC ACACCTCCTA CAAGACCAAG AAGCCCGTGA CCATGCCCCC
          481 CAACCACGTG GTGGAGCACC GCATCGCCAG AACCGACCTG GACAAGGGCG GCAACAGCGT
          541 GCAGCTGACC GAGCACGCCG TGGCCCACAT CACCTCCGTG GTGCCCTTCG GATATCCATC
          601 ACACTGGCGG CCGCTCGAGA TGAATCCCAG TATGAAGCAG AAACAAGAAG AAATCAAAGA
          661 GAATATAAAG AATAGTTCTG TCCCNAGAAG AACTCTGAAG ATGATTCAGC CTTCTGCATC
          721 TGGATCTCTT GTTGGAAGAG AAAATGAGCT GTCCGCAGGC TTGTCCAAAA GGAAACATCG
          781 GANTGACCAC TTAACATCTA CAACTTCCAG CCCTGGGGTT ATTGTCCCAG AATCTAGTGA
          841 AAATAAAAAT CTTGGAGGAN TCANCCAGGA NTCATTTGAT CTTATGANNN AAGAAAANNC
          901 NTNCTNTCAG NATNNGNNNN GNANTGGNAG AAAAANNGAN AANGGNGCTN NNNNNTANNN
        961 NTNNNNNNNN NN
        //
        Primer: EF1a-N_R
        Region: cPPT_CTS,RRE
        Sequence file: RDB15447_B1K5o.seq check
        >D05018D2_B1K5_1_EF1a-N_R_H02_23_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNGGCGNNGT GCGCTCTGCC CACTGNCGGG CACCGGNNCC
           61 TCACGAAGCT TGNATTAATT CAAAGTGAAT TTCTGCTGTC CCTGTAATAA ACCCGAAAAT
          121 TTTGAATTTT TGTAATTTGT TTTTGTAATT CTTTAGTTTG TATGTCTGTT GCTATTATGT
          181 CTACTATTCT TTCCCCTGCA CTGTACCCCC CAATCCCCCC TTTTCTTTTA AAATTGTGGA
          241 TGAATACTGC CATTTGTAGA TCCGTTCACT AATCGAATGG ATCTGTCTCT GTCTCTCTCT
          301 CCACCTTCTT CTTCTATTCC TTCGGGCCTG TCGGGTCCCC TCGGGGTTGG GAGGTGGGTC
          361 TGAAACGATA ATGGTGAATA TCCCTGCCTA ACTCTATTCA CTATAGAAAG TACAGCAAAA
          421 ACTATTCTTA AACCTACCAA GCCTCCTACT ATCATTATGA ATAATTTTAT ATACCACAGC
          481 CAATTTGTTA TGTTAAACCA ATTCCACAAA CTTGCCCATT TATCTAATTC CAATAATTCT
          541 TGTTCATTCT TTTCTTGCTG GTTTTGCGAT TCTTCAATTA AGGAGTGTAT TAAGCTTGTG
          601 TAATTGTTAA TTTCTCTGTC CCACTCCATC CAGGTCGTGT GATTCCAAAT CTGTTCCAGA
          661 GATTTATTAC TCCAACTAGC ATTCCAAGGC ACAGCAGTGG TGCAAATGAG TTTTCCAGAG
          721 CAACCCCAAA TCCCCAGGAG CTGTTGATCC TTTAGGTATC TTTCCACAGC CAGGATTCTT
          781 GCCTGGAGCT GCTTGATGCC CCAGACTGTG AGTTGCAACA NANGCTGTTG CGCCTCAATA
          841 GCCCTCANCA AATTGTTCTG CTGCTGCACT ATACCAGANA ATAATTGTCT GGNCNGNANC
          901 NNCAGCGNCA TTGNNGCTGC GCCCATANNG NTNNCNGCTG NTCCCNNNNA NNCCNNNNNN
        961 NNAANNNNCN NANTNNCNNN NNNNNNTTTT TNNNNNNNNN N
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl