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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(CA)2.1/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15447
Resource name tFucci(CA)2.1/pCSII-EF
Clone info. (provision suspended)
Lentivirus expression vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Anemonia majano CFP cDNA
Reference of nucleotide sequence LC212777 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering Please visit Ordering instruction.[link] UCSD's MTA is also required. Please visit Additional Forms section.
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Exclusive MTA (for use for not-for-profit academic purpose)[Link]
Remarks Remember that you will be working with samples containing infectious virus.
UCSD's MTA is also required. Please visit Additional Forms section.
The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs).
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15447 tFucci(CA)2.1/pCSII-EF DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15447_A9Fqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Region: EF-1alpha promoter, insert 5'
Sequence file: RDB15447_A9Fqa.seq check
>D05018D2_A9Fq_3_EF1a-C_F_B02_05_ABI24.ab1
    1 CCAGGCATTG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAACACGCTA CCGGTCTCGA GAATTCTGCA
  121 GCGGCCGCGG ATCCACCATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG
  181 AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG
  241 AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA
  301 AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA
  361 AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG
  421 GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG
  481 ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC
  541 CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC TCCGAGCGGA
  601 TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG
  661 GCGGCCACTA CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC
  721 CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC CCACAACGAG GACTACACCA
  781 TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG GACGAGCTGT
  841 ACAAGGGATA TCCATCACAC TGGCGGCCGC TCGAGATGGA GCAGCGCCGC GTCACCGACT
  901 TCTTCGCGCG CCGCCGCCCC GGGGCCCCCC CCGCATCGCG CCGCCCAAGC TGGCCTGCCG
  961 CACCCCCAGC CCCGCCAGGC CCGCACTCCG CGCCCCGGGC CTCCGCTACC AGTGGCAGCC
 1021 GCAAGCGCGC CCGGCCCGCC CGGCCGCCCC CGGGACGCGA CCAGGCCCAG GGCCACGGCC
 1081 GCGCGGTGGC GCGGGCTGTC CGGTGGAACG AAGTTCAGCC CCAAGTTACC CCCGGAGGGT
1141 CCCCAGAAAC ATTCCCCCAA GGC
//
Primer: mCherry-C_F
Region: insert mid
Sequence file: RDB15447_A9Fqb.seq check
>D05018D2_A9Fq_3_mCherry-C_F_D03_12_ABI24.ab1
    1 GCCAGGAAGC CCGTGGCAGC TGCCCGGCGC CTACAACGTC AACATCAAGT TGGACATCAC
   61 CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC
  121 CACCGGCGGC ATGGACGAGC TGTACAAGGG ATATCCATCA CACTGGCGGC CGCTCGAGAT
  181 GGAGCAGCGC CGCGTCACCG ACTTCTTCGC GCGCCGCCGC CCCGGGCCCC CCCGCATCGC
  241 GCCGCCCAAG CTGGCCTGCC GCACCCCCAG CCCCGCCAGG CCCGCACTCC GCGCCCCGGC
  301 CTCCGCTACC AGTGGCAGCC GCAAGCGCGC CCGCCCGCCC GCCGCCCCCG GACGCGACCA
  361 GGCCAGGCCA CCGGCCCGCG CGGCCGCGCG GCTGTCGGTG GACGAGGTTT CCAGCCCCAG
  421 TACCCCCGAG GCCCCAGACA TCCCAGCCTG CCCTTCTCCG GGCCAGAAGA TAAAGAAAAC
  481 CGGTCGCGCC AAGCGCGGCA GCGGCGCCAC CAACTTCAGC CTGCTGAAGC AGGCCGGCGA
  541 CGTGGAGGAG AACCCCGGCC CCCGCGAATT CGCCACCATG GCCCTGTCCA ACAAGTTCAT
  601 CGGCGACGAC ATGAAGATGA CCTACCACAT GGACGGCTGC GTGAACGGCC ACTACTTCAC
  661 CGTGAAGGGC GAGGGCAGCG GCAAGCCCTA CGAGGGCACC CAGACCTCCA CCTTCAAGGT
  721 GACCATGGCC AACGGCGGCC CCCTGGCCTT CTCCTTCGAC ATCCTGTCCA CCGTGTTCAT
  781 GTACGGCAAC CGCTGCTTCA CCGCCTACCC CACCAGCATG CCCGACTACT TCAAGCACGC
  841 CTTCCCCGAC GGCATGTCCT ACGAGAGAAC CTTCACCTAC GAGGACGGCG GCGTGGCCAC
  901 CGCCAGCTGG GAGATCAGCC TGAAGGGCAA CTGCTTCGAG CACAAGTCCA CCTTCCACGG
  961 CGTGAACTTC CCCGCCGACG CCCCGTGATG GGCCAAGAGA CCACCGGCTG GGACCCCTCC
 1021 TTTCGAGAAG ATGACCGTGT GCGACGGCAT CTTGAAGGGC GACGTGACCC GCCTTCTGAT
 1081 GCTGCAAGAC GCGGCAACCT ACGAATGGCA AGTCCACACC TCTACAGACA GAGCACGTAC
1141 ATGCTTCATC ACGATGTGT
//
Primer: PRE_rev
Region: PRE, insert 3'
Sequence file: RDB15447_A9Fqc.seq check
>D05018D2_A9Fq_3_PRE_rev_C01_07_ABI24.ab1
    1 GCGAATCAAA TAGCGTAAAG GAGCAACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
   61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGATTAC
  121 AGCGCCTTTC TCCGTTTTTC TGCCACTTCC TTCCAATACT GAGAGGATGG ATTTTCTTTA
  181 ATCATAAGAT CAAATGACTC CTGGGTGACT CCTCCAAGAT TTTTATTTTC ACTAGATTCT
  241 GGGACAATAA CCCCAGGGCT GGAAGTTGTA GATGTTAAGT GGTCATTCCG ATGTTTCCTT
  301 TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA TGCAGAAGGC
  361 TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC TTTGATTTCT
  421 TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA TGGATATCCG
  481 AAGGGCACCA CGGAGGTGAT GTGGGCCACG GCGTGCTCGG TCAGCTGCAC GCTGTTGCCG
  541 CCCTTGTCCA GGTCGGTTCT GGCGATGCGG TGCTCCACCA CGTGGTTGGG GGGCATGGTC
  601 ACGGGCTTCT TGGTCTTGTA GGAGGTGTGG AACTGGCATC TGTAGTTGCC GCCGCCCTGC
  661 AGCATCAGGA AGGCGGTCAC GTCGCCCTTC AAGATGCCGT CGCACACGGT CATCTTCTCG
  721 AAGGAGGGGT CCCAGCCGGT GGTCTTCTTG GCCATCACGG GGCCGTCGGC GGGGAAGTTC
  781 ACGCCGTGGA AGGTGGACTT GTGCTCGAAG CAGTTGCCCT TCAGGCTGAT CTCCCAGCTG
  841 GCGGTGGCCA CGCCGCCGTC CTCGTAGGTG AAGGTTCTCT CGTAGGACAT GCCGTCGGGG
  901 AAGGCCTGCT TGAAGTAGTC GGGCATGCTG GTGGGGTAGG CGGTGAAGCA GCGGTTGCCG
  961 TACATGAACA CGGTGGACAG GATGTCGAAA GAGAAGCCAG GGGGCCGCCG TTTGGCCATG
 1021 GGTCACCTTG AAAGGTGAGG TCTGGTGCCC TCGTAGGGCT TGCCGCTGCC CCTCGCCCTC
 1081 ACGGTGAGTA GTGGGCCCGG TTCACGCAGC GTCAATGTGT AGGTTCAATC TTCATGTCGT
1141 TCCGCCCGGA ATGAAACTTT GTTTGGCAAC CACAGGC
//
Primer: HIVgp_R1
Region: HIV-1 psi, 5' LTR, CMV promoter
Sequence file: RDB15447_A9Fqd.seq check
>D05018D2_A9Fq_3_HIVgp_R1_E03_15_ABI24.ab1
    1 CAAGCTTCTA TTTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCCTTGCTG TGCGGTGGTC
  181 TTACTTTTGT TTTGCTCTTC CTCTATCTTG TCTAAAGCTT CCTTGGTGTC TTTTATCTCT
  241 ATCCTTTGAT GCACACAATA GAGGGTTGCT ACTGTATTAT ATAATGATCT AAGTTCTTCT
  301 GATCCTGTCT GAAGGGATGG TTGTAGCTGT CCCAGTATTT GTCTACAGCC TTCTGATGTT
  361 TCTAACAGGC CAGGATTAAC TGCGAATCGT TCTAGCTCCC TGCTTGCCCA TACTATATGT
  421 TTTAATTTAT ATTTTTTCTT TCCCCCTGGC CTTAACCGAA TTTTTTCCCA TCGCGATCTA
  481 ATTCTCCCCC GCTTAATACT GACGCTCTCG CACCCATCTC TCTCCTTCTA GCCTCCGCTA
  541 GTCAAAATTT TTGGCGTACT CACCAGTCGC CGCCCCTCGC CTCTTGCCGT GCGCGCTTCA
  601 GCAAGCCGAG TCCTGCGTCG AGAGAGCTCC TCTGGTTTCC CTTTCGCTTT CAAGTCCCTG
  661 TTCGGGCGCC ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
  721 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
  781 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
  841 ACCAGAGAGA CCCAGTACAG GCAAAACGCG CTGCTTATAT AGACCTCCCA CCGTACACGC
  901 CTACCGCCCA TTTGCGTCAA TGGGGCGGAG TTGTTACGAC ATTTTGGAAA GTCCCGTTGA
  961 TTTTGGTGCC AAAACAAACT CCCATTGACG TCAATGGGGT GGAGACTTGG AAATCCCCGT
 1021 GAGTCAAACC GCTATCCACG CCCATTGATG TACTGCCAAA ACCGCATCAC CATGGTAATA
 1081 GCGATGACTA ATACGTAGAT GTACTGCCAG TAGGAAAGTC CCATTAGGTC ATGTACTGGC
1141 ATAATGGCAA GCGGGGCCAT TTACCGGTTC CATTGTGGAA CGGTTCTCA
//
Primer: BGH_rev2
Region: BGH poly(A) signal, 3' LTR
Sequence file: RDB15447_A9Fqe.seq check
>D05018D2_A9Fq_3_BGH_rev2_F03_18_ABI24.ab1
    1 CTTTAAGGAT AGATGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
  121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
  181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
  241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
  301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
  361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
  421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
  481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
  541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
  601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
  661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
  721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC
  781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAAGC
  841 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGCCGCGG GAAGGAAGGT CCGCTGGATT
  901 GAGGGCCGAA AGGGACGTAG CAGAAGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGCG
  961 AGCAGCCATG GAAAGGACGT CAGCTTCCCC GACACACCAC GGAAATTGTC AGTGCCCAAC
 1021 AGCCGAGCCC CTGTCCAGCA GCGGGCAGGC AGCGCGATGA GTTCCGCGTG CAATAGGGAG
 1081 GGGAAGGCGA AGGTCCGAAA GGAGCTGACG TGATGCAATG CCCCAATCAG TTGGGGTGCG
1141 TCAGCAACCC ACAGTGTCAC ACAACCAACG G
//
Primer: SV40pro_F
Region: SV40 pro/ori, Zeo, SV40 poly(A) signal
Sequence file: RDB15447_A9Fqf.seq check
>D05018D2_A9Fq_3_SV40pro_F_F01_16_ABI24.ab1
    1 ACTCTGGTCC CGTAAACATT CAGAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAGCACG TGTTGACAAT
  121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG
  181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG
  241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG
  301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC
  361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC
  421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG
  481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG
  541 GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG
  601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG
  661 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC
  721 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG
  781 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG
  841 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC
  901 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC
  961 ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
 1021 GCATTTAATG AATCGGCCCA ACGCGCGGGG AGAGGCGGGT TTGCGTATTG GGGCGCTCTT
 1081 CCGCTTCTCG CTCACTGACT CGCTGCGCTC GGTCGCTCGG CTGCGGCAGC CGGTATCAGC
1141 CTTCACTCAA AAGGCCGTTA ATAACGGGTT TATTTT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl