RDB15782 - pAAV2 SynTetOff Venus-Akaluc

Catalog number	RDB15782
Resource name	pAAV2 SynTetOff Venus-Akaluc
Clone info.	Expression vector of Akaluc luciferase. This clone was constructed based on the pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA vector (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017). The following 2 commercial compounds have been demonstrated to be well catalyzed by Akaluc in vivo: (1) TokeOni (AkaLumine-HCl), Sigma-Aldrich. Product No.: 808350. (2) AkaLumine-HCl, Wako Pure Chemical Industry. Product No.: 012-26701, 018-26703. Patent application PCT/JP2017/030491 submitted by RIKEN that covers the creation and use of Akaluc.
Vector backbone	pAAV2-DEST(r) (Plasmid)
Gene/insert name	firefly (Photinus pyralis) luciferase cDNA
Reference of nucleotide sequence	LC320664 (DDBJ accession number)
Depositor	Miyawaki, Atsushi \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA vector (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.

Terms and conditions set forth by the DEPOSITOR

1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

Additional terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA vector (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
付加的提供条件	利用者は研究成果の公表にあたって、pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA の文献 (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017) を引用し、順天堂大学日置寛之先生への謝辞の表明を必要とする。本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
備考	提供条件の言語は英語のみ。

References and tips

Sequence information

Catalog #	Resource name	Availability
RDB15782	pAAV2 SynTetOff Venus-Akaluc	This clone has been withdrawn.

Featured content	Dr. Miyawaki's resources (English text)

original	Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
original	Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1.

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Primer: CSII_CMV5_v2	RDB15782_A8B8a.seq
>D04498K4_A8B8_1_CSII_CMV5_V2_A01_01_ABI08.ab1 1 TGGTGGGAAA TCGGAGCTCG GTACCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC 61 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT 121 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGC TGATCTGCAC 181 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGGGCT ACGGCCTGCA 241 GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC 301 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG 361 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA 421 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA 481 CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGCCA 541 CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG 601 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC TACCAGTCCG CCCTGAGCAA 661 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT 721 CACTCTCGGC ATGGACGAGC TGTACAAGGG ATCCATGGAA GATGCCAAAA ACATTAAGAA 781 GGGCCCAGCG CCGTTCTACC CACTCGAAGA CGGGACCGCC GGCGAGCAGC TGCACAAAGC 841 CATGAAGCGC TACGCCCTGG TGCCCGGCGC CATCGCCTTT ACCGACGCAC ATATTCAGGT 901 GGACGTTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG CTATGAGGCG 961 CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC GAGAATAGCT CGCAGTTCTT 1021 TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGG TGACCCAGGC TAACGACATC 1081 TACACGAGCG CGAGCCTGGC TGGCACCAGC AATGGGGTCA TCAAGTCCAA GGCGAC //
Primer: Akaluc 1215-1243	RDB15782_A8B8b.seq
>D04498K4_A8B8_1_Akaluc 1215-1243_D01_04_ABI08.ab1 1 TCCTGCGGGC GCTCGCCTAC TGGGACGAGG ACGAGCACTT CTTCATCGTG GACCGGCTGA 61 AGAGCCTGAT CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG GGCATCCTGC 121 TGCAACACCC CTACATCTTC GACGCCGGAG TCGCCGGCCT GCCCGACGAC GATGCCGGCG 181 AGCTGCCCGC CGCAGTCGTC GTGTTGGAAC ACGGTAAAAC CATGACCGAG AAAGAGATCG 241 TGGACTATGT GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT GTTGTGTTTG 301 TGGATGAAGT CCCTAGAGGA TCGACCGGCA AGTTAGACGC CCGCAAGATC CGCGAGATTC 361 TCACTAAGGC CAAGAAGGAC GGCAAGATCG CCGTGTAAAC GCGTCTGTGC CTTCTAGTTG 421 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC 481 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC 541 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG 601 GCATGCTGGG GATGCGGTGG GCTCTATGGG CGGCCGCGTC GAGATATCTA GACCCAGCTT 661 TCTTGTACAA AGTGGTACGC GTGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGCCACTC 721 CCTCTCTGCG CGCT //
Primer: Venus-C_F2	RDB15782_A8B8c.seq
>D04498K4_A8B8_1_Venus-C_F2_B01_02_ABI08.ab1 1 TGCATTGGAC GGCGCAGGAA TCACTCTCGG CATGGACGAG CTGTACAAGG GATCCATGGA 61 AGATGCCAAA AACATTAAGA AGGGCCCAGC GCCGTTCTAC CCACTCGAAG ACGGGACCGC 121 CGGCGAGCAG CTGCACAAAG CCATGAAGCG CTACGCCCTG GTGCCCGGCG CCATCGCCTT 181 TACCGACGCA CATATTCAGG TGGACGTTAC CTACGCCGAG TACTTCGAGA TGAGCGTTCG 241 GCTGGCAGAA GCTATGAGGC GCTATGGGCT GAATACAAAC CATCGGATCG TGGTGTGCAG 301 CGAGAATAGC TCGCAGTTCT TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGT 361 GGCCCCAGCT AACGACATCT ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATCAGCCA 421 GCCCACCGTC GTATTCGTGA GCAAGAAAGG GCTGCGAAAG GTCCTCAACG TGCAAAAGAA 481 GCTACCGATC ATACGAAAGA TCATCATCAT GGATAGCAAG ACCGACTACC AGGGCTTCCA 541 AAGCATGTAC ACCTTCGTGA CTTCCCATTT GCCACCCAGC TTCAACGAGT ACGACTTCGT 601 GCCCGAGAGC TTCGACCGGG ACAAAACCAT CGCCCTGATC ATGAACAGTA GTGGTAGTAC 661 AGGATTACCC AAGGGCGTAG CCCTACCGCA CCGCACCGCT TGTGTCCGAT TCAGTCATGC 721 CCGCGACCCC ATCTTCGGCT ACCAGAACAT CCCCGACACC GCTATCCTCA GCGTGGTGCC 781 ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT 841 CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT 901 TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTGCCTC GCTAAGAGCA CTCTCATCGA 961 CAAGTACGAC CTAAAGCAGC TTGCGCGAGA TCGCCCAGCG GGCGGGGGCG CCCGCTCAGC 1021 AAGAGGGTAG GTGAGGGCCG GTGGACCAAC GCTTCCGCCC TACCAGCAAT CCGCCAGGGC 1081 TATGGCCCTG ACAGGAACAC CAACGGTCGG TCCATGATTC ACCCCCCCGG AAGGGGGGGA 1141 ACCGGT //
Primer: Akaluc 576-593	RDB15782_A8B8d.seq
>D04498K4_A8B8_1_Akaluc 576-593_C01_03_ABI08.ab1 1 CAAAAAAACC AGGGGGCGTA GCCCTACCGC ACCGCACCGC TTGTGTCCGA TTCAGTCATG 61 CCCGCGACCC CATCTTCGGC TACCAGAACA TCCCCGACAC CGCTATCCTC AGCGTGGTGC 121 CATTTCACCA CGGCTTCGGC ATGTTCACCA CGCTGGGCTA CTTGATCTGC GGCTTTCGGG 181 TCGTGCTCAT GTACCGCTTC GAGGAGGAGC TATTCTTGCG CAGCTTGCAA GACTATAAGA 241 TTCAATCTGC CCTGCTGGTG CCCACACTAT TTAGCTGCCT CGCTAAGAGC ACTCTCATCG 301 ACAAGTACGA CCTAAGCAGC TTGCGCGAGA TCGCCAGCGG CGGGGCGCCG CTCAGCAAGG 361 AGGTAGGTGA GGCCGTGGCC AAACGCTTCC GCCTACCAGG CATCCGCCAG GGCTATGGCC 421 TGACAGAAAC AACCAACGCC GTCATGATCA CCCCCGAGGG GGACCGTAAG CCTGGCTCAG 481 TAGGCAAGGT GGTGCCCTTC TTCGAGGCTA AGGTGGTAGA CTTGGTCACC GGTAAGACAC 541 TGGGTGTGAA CCAGCGCGGT GAGCTGTGCG TCCGTGGCCC CATGATCATG AGCGGCTACG 601 TTAACAACCC CGAGGCTACG AACGCTCTCA TCGACAAGGA CGGCTGGCTG CACAGCGGCG 661 ACATCGCCTA CTGGGACGAG GACGAGCACT TCTTCATCGT GGACCGGCTG AAGAGCCTGA 721 TCAAATACAA GGGCTACCAG GTAGCCCCAG CCGAACTGGA GGGCATCCTG CTGCAACACC 781 CCTACATCTT CGACGCCGGA GTCGCCGGCC TGCCCGACGA CGATGCCGGC GAGCTGCCCG 841 CCGCAGTCGT CGTGTTGGAA CACGGTAAAA CCATGACCGA GAAAGAGATC GTGGACTATG 901 TGGCCAGCCA GGTTACAACC GCCAAGAAGC TGCGCGGTGG TGTTGTGTTT GTGGATGAAG 961 TCCCTAGAGG GATCGACCGG CAAGTTAGAC GCCCGCAAGG ATCCGCGAGA TTCTCACTAA 1021 AGGCCCAAGA AGGACGGCAG ATCGCCCGTG TAAACGCGGT CTGTGCCTTC TAGCTGCAGT 1081 CATCTATTGA TTGCCCGACC CCGATGGCAT TCGTTGGACC CTGGGAAGGG TGGCCCACAT 1141 C //
Primer: tTA-Ad R	RDB15782_A8B8e.seq
>D04498K4_A8B8_1_tTA-AdR_E01_05_ABI08.ab1 1 GATCCGGGGG GCAGGAGGTT TATGGACTTT GCTCTTGTCC AGTCTAGACA TGGTGAATTC 61 GATATCAAGC TTATCGATAC CGTCCGCCGC AGCGCAGATG GTCGCGCCCG TGCCCCCCTA 121 TCTCGCGCCT CGCGTGGTGC GGTCCGGCTG GGCCGGCGGC GGCGCGGACG CGACCAAGGT 181 GGCCGGGAAG GGGAGTTTGC GGGGGACCGG CGAGTGACGT CAGCGCGCCT TCAGTGCTGA 241 GGCGGCGGTG GCGCGCGCCG CCAGGCGGGG GCGAAGGCAC TGTCCGCGGT GCTGAAGCTG 301 GCAGTGCGCA CGCGCCTCGC CGCATCCTGT TTCCCCTCCC CCTCTCTGAT AGGGGATGCG 361 CAATTTGGGG AATGGGGGTT GGGTGCTTGT CCAGTGGGTC GGGGTCGGTC GTCAGGTAGG 421 CACCCCCACC CCGCCTCATC CTGGTCCTAA AACCCACTTG CACTCATACG CAGGGCCCTC 481 TGCAGCTCGA CCGCGCCGCC CCGGGCGGCT CGCTGCTGCC CCCTAGCGGG GGAGGGACGT 541 AATTACATCC CTGGGGGCTT TGGGGGGGCT CGAGTTTACT CCCTATCAGT GATAGAGAAC 601 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGATG TCGAGTTTAC TCCCTATCAG 661 TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT 721 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTATCC CTATCAGTGA TAGAGAACGT 781 ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGGTAGGC GTGTACGGTG 841 GGAGGCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GAATTCGAGC 901 TCGGTACCGC CACCATGGTG AGCAAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC 961 CTGGTCGAGC TGGACGGGCG ACGTAAACGG GCCACAAGTT TCAGCGTGTC CGGGCGAGGG 1021 CGAGGCGATG CCACCTACGG CAAGCTGACC CTGAAGCTGA TCTGCACCAC CGGGCCAGCT 1081 GCCGTGCCCT GACCATCTTC GTGACAACCC TGGGCCTACC GACCCTGCCA GTGCCTTTCG 1141 AACCCCGC //
Primer: tTA-Ad F	RDB15782_A8B8f.seq
>D04498K4_A8B8_1_tTA-AdF_F01_06_ABI08.ab1 1 ACATTCTTTT CGAGCTGGAA CTAATCATAT GTGGCCTGGA GAAACAGCTA AAGTGCGAAA 61 GCGGCGGGCC GGCCGACGCC CTTGACGATT TTGACTTAGA CATGCTCCCA GCCGATGCCC 121 TTGACGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT TGACGATTTT GACCTTGACA 181 TGCTCCCCGG GTAACTAAGT AAGGATCTCA GACATGATAA GATACATTGA TGAGTTTGGA 241 CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT 301 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT 361 TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC 421 AAATGTGGTA AGATCCAAAG CCTGCTTTTT TGTACAAACT TGTCACGTGC GGACCGAGCG 481 GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCT //

original	Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
original	Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1.
reference	Nasu, Y., Unnaturally aglow with a bright inner light. Science 359 (6378): 868-869 (2018). PMID 29472468.
reference	Kuchimaru, T., A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat. Commun. 7: 11856 (2016). PMID 27297211.
reference	Iwano, S., Development of simple firefly luciferin analogs emitting blue, green, red, and near-infrared biological window light. Tetrahedron 69 (19): 3847-3856 (2013).
reference	Iwano, S., Development of simple firefly luciferin analogs emitting blue, green, red, and near-infrared biological window light. Tetrahedron 69 (19): 3847-3856 (2013).