Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAAV2 SynTetOff Venus-Akaluc

Expression vector of Akaluc luciferase.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA vector (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
付加的提供条件	利用者は研究成果の公表にあたって、pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA の文献 (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017) を引用し、順天堂大学日置寛之先生への謝辞の表明を必要とする。 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15782_A8B8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: tTA-Ad F Region: insert 5'(tTA-Advanced,SV40 pA,attB1),AAV2 ITR Sequence file: RDB15782_A8B8g.seq
>D04498K4_A8B8_1_tTA-AdF_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTAT GTGGCCTGGA NAAACAGCTA AAGTGCGAAA    61 GCGGCGGGCC GGCCGACGCC CTTGACGATT TTGACTTAGA CATGCTCCCA GCCGATGCCC   121 TTGACGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT TGACGATTTT GACCTTGACA   181 TGCTCCCCGG GTAACTAAGT AAGGATCTCA GACATGATAA GATACATTGA TGAGTTTGGA   241 CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT   301 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT   361 TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC   421 AAATGTGGTA AGATCCAAAG CCTGCTTTTT TGTACAAACT TGTCACGTGC GGACCGAGCG 481 GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGN //
Primer: Akaluc 1215-1243 Region: insert 3'(Akaluc 3',bGH pA),attB2, AAV2 ITR Sequence file: RDB15782_A8B8h.seq
>D04498K4_A8B8_1_Akaluc 1215-1243_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNGCCNNN NGGGNNGANG NNGNNCNCTT CTTCNTCGTG GANCGGCTGA    61 AGAGCCTGAT CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG GGCATCCTGC   121 TGCAACACCC CTACATCTTC GACGCCGGAG TCGCCGGCCT GCCCGACGAC GATGCCGGCG   181 AGCTGCCCGC CGCAGTCGTC GTGTTGGAAC ACGGTAAAAC CATGACCGAG AAAGAGATCG   241 TGGACTATGT GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT GTTGTGTTTG   301 TGGATGAAGT CCCTAGAGGA TCGACCGGCA AGTTAGACGC CCGCAAGATC CGCGAGATTC   361 TCACTAAGGC CAAGAAGGAC GGCAAGATCG CCGTGTAAAC GCGTCTGTGC CTTCTAGTTG   421 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC   481 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC   541 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG   601 GCATGCTGGG GATGCGGTGG GCTCTATGGG CGGCCGCGTC GAGATATCTA GACCCAGCTT   661 TCTTGTACAA AGTGGTACGC GTGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGNNNNNC 721 CCTCTCTGCG CGNN //
Primer: tTA-Ad R Region: insert mid(SYN pro,insulator,tiht TRE pro) Sequence file: RDB15782_A8B8i.seq
>D04498K4_A8B8_1_tTA-AdR_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT NNNNNNNTTT GCTCNNNNNN NGTCTAGACN TGGTGAATTC    61 GATATCAAGC TTATCGATAC CGTCCGCCGC AGCGCAGATG GTCGCGCCCG TGCCCCCCTA   121 TCTCGCGCCT CGCGTGGTGC GGTCCGGCTG GGCCGGCGGC GGCGCGGACG CGACCAAGGT   181 GGCCGGGAAG GGGAGTTTGC GGGGGACCGG CGAGTGACGT CAGCGCGCCT TCAGTGCTGA   241 GGCGGCGGTG GCGCGCGCCG CCAGGCGGGG GCGAAGGCAC TGTCCGCGGT GCTGAAGCTG   301 GCAGTGCGCA CGCGCCTCGC CGCATCCTGT TTCCCCTCCC CCTCTCTGAT AGGGGATGCG   361 CAATTTGGGG AATGGGGGTT GGGTGCTTGT CCAGTGGGTC GGGGTCGGTC GTCAGGTAGG   421 CACCCCCACC CCGCCTCATC CTGGTCCTAA AACCCACTTG CACTCATACG CAGGGCCCTC   481 TGCAGCTCGA CCGCGCCGCC CCGGGCGGCT CGCTGCTGCC CCCTAGCGGG GGAGGGACGT   541 AATTACATCC CTGGGGGCTT TGGGGGGGCT CGAGTTTACT CCCTATCAGT GATAGAGAAC   601 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGATG TCGAGTTTAC TCCCTATCAG   661 TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT   721 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTATCC CTATCAGTGA TAGAGAACGT   781 ATGTCGAGTT TACTCCCTAT CAGTGATAGA GANNGTATGT CGAGGTANGC GTGTACGGTG   841 NNNNGCCTAT ATAAGCAGAN CTCGTTTANT GAACCGTCAG ATCGCCNNGA NAANTCNAGC   901 TCGGNACCGC CNCCNTNGNG AGCNANGGNC GANGANCTNN NCNCCGGGGN NGNGCCNNTC 961 CNNNNNNANN TGGNNNGNCN NNGTNAANNG NNCNCAAGNT NNANNNNNNN NNN //
Primer: EGFP-N_R2 Region: insert mid(Venus 5',tight TRE pro,Insulator,SYN pro) Sequence file: RDB15782_A8B8j.seq
>15782_15782_B2GdF_1_EGFP-N_R2_A07_01_ABI08.ab1     1 ANNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNCAN GCCGNANCCC AGGGTGGTCA    61 CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATCAGCTTC AGGGTCAGCT   121 TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT   181 CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA   241 CCATGGTGGC GGTACCGAGC TCGAATTCTC CAGGCGATCT GACGGTTCAC TAAACGAGCT   301 CTGCTTATAT AGGCCTCCCA CCGTACACGC CTACCTCGAC ATACGTTCTC TATCACTGAT   361 AGGGAGTAAA CTCGACATAC GTTCTCTATC ACTGATAGGG ATAAACTCGA CATACGTTCT   421 CTATCACTGA TAGGGAGTAA ACTCGACATA CGTTCTCTAT CACTGATAGG GAGTAAACTC   481 GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA TCGTTCTCTA TCACTGATAG   541 GGAGTAAACT CGACATACGT TCTCTATCAC TGATAGGGAG TAAACTCGAG CCCCCCCAAA   601 GCCCCCAGGG ATGTAATTAC GTCCCTCCCC CGCTAGGGGG CAGCAGCGAG CCGCCCGGGG   661 CGGCGCGGTC GAGCTGCAGA GGGCCCTGCG TATGAGTGCA AGTGGGTTTT AGGACCAGGA   721 TGANNNGGGG TGGGGGTGCC TACCTGACNA CCGACNNNNA NCCANTGGAC AANNNNNNAN 781 NNNNNNNNN //
Primer: CSII_CMV5_v2 Region: insert mid(Venus mid) Sequence file: RDB15782_A8B8k.seq
>D04498K4_A8B8_1_CSII_CMV5_V2_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNCNNN NNNNNGCNNC CNTGGTGAGC AAGGGCGAGG NGCTGTTCNC    61 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT   121 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGC TGATCTGCAC   181 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGGGCT ACGGCCTGCA   241 GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC   301 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG   361 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA   421 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA   481 CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGCCA   541 CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG   601 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC TACCAGTCCG CCCTGAGCAA   661 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGNT   721 CACTCTCGGC ATGGACGAGC TGTACAAGGG ATCCATGGAA GATGCCAAAA ACATTAAGAA   781 GGGCCCAGCG CCGTTCTACC CACTCGAAGA NGGNNNCGCC GGCGANCAGC TGCACNAAGC   841 CNTGAAGCGC TACGCCCTGG TGCCCGGCGC CNTCGCCTTT ANCGACGCAC ATATTCANGN   901 GGNCGTTANC TACGCCNANN ACTTCNAGNT NNNCGTTCNG NNGGNAGANN NTANNNNNNN 961 CTNNNGGNTN NNNNNNNNN //
Primer: mVenus-M_F Region: insert mid(Venus 3',Akaluc 5') Sequence file: RDB15782_A8B8l.seq
>15782_15782_B2GdF_1_mVenus-M_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNANNNNN NCNNGGNNNN NTTNNAGATC CGCCNCAACA TCGAGGACGG    61 CGGCGTGCAG CTNNNCNACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT   121 GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA   181 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA   241 CGAGCTGTAC AAGGGATCCA TGGAAGATGC CAAAAACATT AAGAAGGGCC CAGCGCCGTT   301 CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC AAAGCCATGA AGCGCTACGC   361 CCTGGTGCCC GGCGCCATCG CCTTTACCGA CGCACATATT CAGGTGGACG TTACCTACGC   421 CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG AGGCGCTATG GGCTGAATAC   481 AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTCGCAG TTCTTCATGC CCGTGTTGGG   541 TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC ATCTACAACG AGCGCGAGCT   601 GCTGAACAGC ATGGGCATCA GCCNGCCCAC CGTCGTATTC GTGAGCAANA AANGGNTGCG   661 AAANGNCCTC NANNNGCAAA ANAANCNACC NATCNNNCNA ANNNNNATCN TNNTGGANNN 721 NNNGANCNAN NNNCNNNNNN NNNN //
Primer: Venus-C_F2 Region: insert mid(Akaluc mid) Sequence file: RDB15782_A8B8m.seq
>15782_15782_B2GdF_1_Venus-C_F2_B07_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNNNNNTCT CGGNNNGGNN GAGCTGTACA AGGGNNCCAT    61 GGAAGANGCC AAAAACATTA AGAAGGGCCC AGCGCCGTTC TACCCACTCG AAGACGGGAC   121 CGCCGGCGAG CAGCTGCACA AAGCCATGAA GCGCTACGCC CTGGTGCCCG GCGCCATCGC   181 CTTTACCGAC GCACATATTC AGGTGGACGT TACCTACGCC GAGTACTTCG AGATGAGCGT   241 TCGGCTGGCA GAAGCTATGA GGCGCTATGG GCTGAATACA AACCATCGGA TCGTGGTGTG   301 CAGCGAGAAT AGCTCGCAGT TCTTCATGCC CGTGTTGGGT GCCCTGTTCA TCGGTGTGGC   361 TGTGGCCCCA GCTAACGACA TCTACAACGA GCGCGAGCTG CTGAACAGCA TGGGCATCAG   421 CCAGCCCACC GTCGTATTCG TGAGCAAGAA AGGGCTGCGA AAGGTCCTCA ACGTGCAAAA   481 GAAGCTACCG ATCATACGAA AGATCATCAT CATGGATAGC AAGACCGACT ACCAGGGCTT   541 CCAAAGCATG TACACCTTCG TGACTTCCCA TTTGCCACCC AGCTTCAACG AGTACGACTT   601 CGTGCCCGAG AGCTTCGACC GGGACAAAAC CATCGCCCTG ATCATGAACA GTAGTGGTAG   661 TACAGGATTA CCCAAGGGCG TAGCCCTACC GCACCGCACC GCTTGTGTCC GATTCAGTCA   721 TGCCCGCGAC CCCATCTTCG GCTACCAGAA CATCCCCGAC ACCGCTATCC TCAGCGTGGT   781 GCCATTTCAC CACGGCTTCN NCATGTTCAC CACGCTGGGC TACTTGATCT GCGGCTTTCG   841 GGTCGTGCTC ATGTACCGCT TCGAGGANGA NCTATTCTTG CGCAGCTTGC AAGANTATAA   901 GANTNNATCT GCCCTGCTGG TGCCCACNNN ANTTAGCTGC CTCGCTAANA NCNNTCTCNT   961 CGACNAGTAC NANCTANNCA GCTTGNNCGA GATNGCNAGC NGNGGGGCNN NNNNCANNNA 1021 NGGAGGNNNN NGAGGNCNNN NNCNAAANNC NTNNNNNNNN NN //
Primer: Akaluc 576-593 Region: insert mid(Akaluc mid) Sequence file: RDB15782_A8B8n.seq
>D04498K4_A8B8_1_Akaluc 576-593_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNGNGNGNN NCNNTACCGC NCCGCACCGC TTGTGTCCGA TTCNGTCATG    61 CCCGCGACCC CATCTTCGGC TACCAGAACA TCCCCGACAC CGCTATCCTC AGCGTGGTGC   121 CATTTCACCA CGGCTTCGGC ATGTTCACCA CGCTGGGCTA CTTGATCTGC GGCTTTCGGG   181 TCGTGCTCAT GTACCGCTTC GAGGAGGAGC TATTCTTGCG CAGCTTGCAA GACTATAAGA   241 TTCAATCTGC CCTGCTGGTG CCCACACTAT TTAGCTGCCT CGCTAAGAGC ACTCTCATCG   301 ACAAGTACGA CCTAAGCAGC TTGCGCGAGA TCGCCAGCGG CGGGGCGCCG CTCAGCAAGG   361 AGGTAGGTGA GGCCGTGGCC AAACGCTTCC GCCTACCAGG CATCCGCCAG GGCTATGGCC   421 TGACAGAAAC AACCAACGCC GTCATGATCA CCCCCGAGGG GGACCGTAAG CCTGGCTCAG   481 TAGGCAAGGT GGTGCCCTTC TTCGAGGCTA AGGTGGTAGA CTTGGTCACC GGTAAGACAC   541 TGGGTGTGAA CCAGCGCGGT GAGCTGTGCG TCCGTGGCCC CATGATCATG AGCGGCTACG   601 TTAACAACCC CGAGGCTACG AACGCTCTCA TCGACAAGGA CGGCTGGCTG CACAGCGGCG   661 ACATCGCCTA CTGGGACGAG GACGAGCACT TCTTCATCGT GGACCGGCTG AAGAGCCTGA   721 TCAAATACAA GGGCTACCAG GTAGCCCCAG CCGAACTGGA GGGCATCCTG CTGCAACACC   781 CCTACATCTT CGACGCCGGA NTCGCCGGCC TGCCCGANGA CGATGCCGGC GANCTGCCCG   841 CCGCANTCGN CGTGTTGGAA CACGGTAAAA CCATGANCGA NAAANANNTC GNGGACTATG   901 TGGCCNGCCN GGNTNNNNNC GCCNAGAANC TGCNCGGNGG TGNNGNNNTT GNNGNTNANN 961 TCCNNANNNG NATCGNNNGN NNNNNNAGNN GCCNGCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.08.05

GNP_filter3_RDBDEP_html_220727.pl