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pAAV2 SynTetOff Venus-Akaluc

Expression vector of Akaluc luciferase.

Catalog number RDB15782
Resource name pAAV2 SynTetOff Venus-Akaluc
Clone info. Expression vector of Akaluc luciferase.
Comment This clone was constructed based on the pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA vector (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017).
The following 2 commercial compounds have been demonstrated to be well catalyzed by Akaluc in vivo:
(1) TokeOni (AkaLumine-HCl), Sigma-Aldrich. Product No.: 808350.
(2) AkaLumine-HCl, Wako Pure Chemical Industry. Product No.: 012-26701, 018-26703.
Patent application PCT/JP2017/030491 submitted by RIKEN that covers the creation and use of Akaluc.
Vector backbone pAAV2-DEST(r) (Plasmid)
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Reference of insert sequence LC320664 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
firefly (Photinus pyralis) luciferase

          Reference sequence
            
           
          Sequence (full) RDB15782zzk02.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018).
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Additional terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA vector (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested.
          The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018).
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          付加的提供条件 利用者は研究成果の公表にあたって、pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA の文献 (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017) を引用し、順天堂大学日置寛之先生への謝辞の表明を必要とする。
          本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考 提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15782 pAAV2 SynTetOff Venus-Akaluc DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
          original Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0081e (English text)
          Featured content Dnaconda's recommendation EXR0081j (Japanese text)
          Featured content Higher intensity luciferase (English text)
          Featured content 高発光ルシフェラーゼ (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15782_A8B8p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: tTA-Ad F
          Region: insert 5'(tTA-Advanced,SV40 pA,attB1),AAV2 ITR
          Sequence file: RDB15782_A8B8g.seq check
          >D04498K4_A8B8_1_tTA-AdF_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTAT GTGGCCTGGA NAAACAGCTA AAGTGCGAAA
             61 GCGGCGGGCC GGCCGACGCC CTTGACGATT TTGACTTAGA CATGCTCCCA GCCGATGCCC
            121 TTGACGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT TGACGATTTT GACCTTGACA
            181 TGCTCCCCGG GTAACTAAGT AAGGATCTCA GACATGATAA GATACATTGA TGAGTTTGGA
            241 CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT
            301 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT
            361 TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC
            421 AAATGTGGTA AGATCCAAAG CCTGCTTTTT TGTACAAACT TGTCACGTGC GGACCGAGCG
          481 GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGN
          //
          Primer: Akaluc 1215-1243
          Region: insert 3'(Akaluc 3',bGH pA),attB2, AAV2 ITR
          Sequence file: RDB15782_A8B8h.seq check
          >D04498K4_A8B8_1_Akaluc 1215-1243_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNGCCNNN NGGGNNGANG NNGNNCNCTT CTTCNTCGTG GANCGGCTGA
             61 AGAGCCTGAT CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG GGCATCCTGC
            121 TGCAACACCC CTACATCTTC GACGCCGGAG TCGCCGGCCT GCCCGACGAC GATGCCGGCG
            181 AGCTGCCCGC CGCAGTCGTC GTGTTGGAAC ACGGTAAAAC CATGACCGAG AAAGAGATCG
            241 TGGACTATGT GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT GTTGTGTTTG
            301 TGGATGAAGT CCCTAGAGGA TCGACCGGCA AGTTAGACGC CCGCAAGATC CGCGAGATTC
            361 TCACTAAGGC CAAGAAGGAC GGCAAGATCG CCGTGTAAAC GCGTCTGTGC CTTCTAGTTG
            421 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC
            481 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC
            541 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG
            601 GCATGCTGGG GATGCGGTGG GCTCTATGGG CGGCCGCGTC GAGATATCTA GACCCAGCTT
            661 TCTTGTACAA AGTGGTACGC GTGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGNNNNNC
          721 CCTCTCTGCG CGNN
          //
          Primer: tTA-Ad R
          Region: insert mid(SYN pro,insulator,tiht TRE pro)
          Sequence file: RDB15782_A8B8i.seq check
          >D04498K4_A8B8_1_tTA-AdR_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNT NNNNNNNTTT GCTCNNNNNN NGTCTAGACN TGGTGAATTC
             61 GATATCAAGC TTATCGATAC CGTCCGCCGC AGCGCAGATG GTCGCGCCCG TGCCCCCCTA
            121 TCTCGCGCCT CGCGTGGTGC GGTCCGGCTG GGCCGGCGGC GGCGCGGACG CGACCAAGGT
            181 GGCCGGGAAG GGGAGTTTGC GGGGGACCGG CGAGTGACGT CAGCGCGCCT TCAGTGCTGA
            241 GGCGGCGGTG GCGCGCGCCG CCAGGCGGGG GCGAAGGCAC TGTCCGCGGT GCTGAAGCTG
            301 GCAGTGCGCA CGCGCCTCGC CGCATCCTGT TTCCCCTCCC CCTCTCTGAT AGGGGATGCG
            361 CAATTTGGGG AATGGGGGTT GGGTGCTTGT CCAGTGGGTC GGGGTCGGTC GTCAGGTAGG
            421 CACCCCCACC CCGCCTCATC CTGGTCCTAA AACCCACTTG CACTCATACG CAGGGCCCTC
            481 TGCAGCTCGA CCGCGCCGCC CCGGGCGGCT CGCTGCTGCC CCCTAGCGGG GGAGGGACGT
            541 AATTACATCC CTGGGGGCTT TGGGGGGGCT CGAGTTTACT CCCTATCAGT GATAGAGAAC
            601 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGATG TCGAGTTTAC TCCCTATCAG
            661 TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT
            721 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTATCC CTATCAGTGA TAGAGAACGT
            781 ATGTCGAGTT TACTCCCTAT CAGTGATAGA GANNGTATGT CGAGGTANGC GTGTACGGTG
            841 NNNNGCCTAT ATAAGCAGAN CTCGTTTANT GAACCGTCAG ATCGCCNNGA NAANTCNAGC
            901 TCGGNACCGC CNCCNTNGNG AGCNANGGNC GANGANCTNN NCNCCGGGGN NGNGCCNNTC
          961 CNNNNNNANN TGGNNNGNCN NNGTNAANNG NNCNCAAGNT NNANNNNNNN NNN
          //
          Primer: EGFP-N_R2
          Region: insert mid(Venus 5',tight TRE pro,Insulator,SYN pro)
          Sequence file: RDB15782_A8B8j.seq check
          >15782_15782_B2GdF_1_EGFP-N_R2_A07_01_ABI08.ab1
              1 ANNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNCAN GCCGNANCCC AGGGTGGTCA
             61 CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATCAGCTTC AGGGTCAGCT
            121 TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT
            181 CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA
            241 CCATGGTGGC GGTACCGAGC TCGAATTCTC CAGGCGATCT GACGGTTCAC TAAACGAGCT
            301 CTGCTTATAT AGGCCTCCCA CCGTACACGC CTACCTCGAC ATACGTTCTC TATCACTGAT
            361 AGGGAGTAAA CTCGACATAC GTTCTCTATC ACTGATAGGG ATAAACTCGA CATACGTTCT
            421 CTATCACTGA TAGGGAGTAA ACTCGACATA CGTTCTCTAT CACTGATAGG GAGTAAACTC
            481 GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA TCGTTCTCTA TCACTGATAG
            541 GGAGTAAACT CGACATACGT TCTCTATCAC TGATAGGGAG TAAACTCGAG CCCCCCCAAA
            601 GCCCCCAGGG ATGTAATTAC GTCCCTCCCC CGCTAGGGGG CAGCAGCGAG CCGCCCGGGG
            661 CGGCGCGGTC GAGCTGCAGA GGGCCCTGCG TATGAGTGCA AGTGGGTTTT AGGACCAGGA
            721 TGANNNGGGG TGGGGGTGCC TACCTGACNA CCGACNNNNA NCCANTGGAC AANNNNNNAN
          781 NNNNNNNNN
          //
          Primer: CSII_CMV5_v2
          Region: insert mid(Venus mid)
          Sequence file: RDB15782_A8B8k.seq check
          >D04498K4_A8B8_1_CSII_CMV5_V2_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNCNNN NNNNNGCNNC CNTGGTGAGC AAGGGCGAGG NGCTGTTCNC
             61 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT
            121 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGC TGATCTGCAC
            181 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGGGCT ACGGCCTGCA
            241 GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC
            301 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG
            361 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA
            421 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA
            481 CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGCCA
            541 CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG
            601 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC TACCAGTCCG CCCTGAGCAA
            661 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGNT
            721 CACTCTCGGC ATGGACGAGC TGTACAAGGG ATCCATGGAA GATGCCAAAA ACATTAAGAA
            781 GGGCCCAGCG CCGTTCTACC CACTCGAAGA NGGNNNCGCC GGCGANCAGC TGCACNAAGC
            841 CNTGAAGCGC TACGCCCTGG TGCCCGGCGC CNTCGCCTTT ANCGACGCAC ATATTCANGN
            901 GGNCGTTANC TACGCCNANN ACTTCNAGNT NNNCGTTCNG NNGGNAGANN NTANNNNNNN
          961 CTNNNGGNTN NNNNNNNNN
          //
          Primer: mVenus-M_F
          Region: insert mid(Venus 3',Akaluc 5')
          Sequence file: RDB15782_A8B8l.seq check
          >15782_15782_B2GdF_1_mVenus-M_F_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNANNNNN NCNNGGNNNN NTTNNAGATC CGCCNCAACA TCGAGGACGG
             61 CGGCGTGCAG CTNNNCNACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT
            121 GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA
            181 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA
            241 CGAGCTGTAC AAGGGATCCA TGGAAGATGC CAAAAACATT AAGAAGGGCC CAGCGCCGTT
            301 CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC AAAGCCATGA AGCGCTACGC
            361 CCTGGTGCCC GGCGCCATCG CCTTTACCGA CGCACATATT CAGGTGGACG TTACCTACGC
            421 CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG AGGCGCTATG GGCTGAATAC
            481 AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTCGCAG TTCTTCATGC CCGTGTTGGG
            541 TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC ATCTACAACG AGCGCGAGCT
            601 GCTGAACAGC ATGGGCATCA GCCNGCCCAC CGTCGTATTC GTGAGCAANA AANGGNTGCG
            661 AAANGNCCTC NANNNGCAAA ANAANCNACC NATCNNNCNA ANNNNNATCN TNNTGGANNN
          721 NNNGANCNAN NNNCNNNNNN NNNN
          //
          Primer: Venus-C_F2
          Region: insert mid(Akaluc mid)
          Sequence file: RDB15782_A8B8m.seq check
          >15782_15782_B2GdF_1_Venus-C_F2_B07_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGNNNNNTCT CGGNNNGGNN GAGCTGTACA AGGGNNCCAT
             61 GGAAGANGCC AAAAACATTA AGAAGGGCCC AGCGCCGTTC TACCCACTCG AAGACGGGAC
            121 CGCCGGCGAG CAGCTGCACA AAGCCATGAA GCGCTACGCC CTGGTGCCCG GCGCCATCGC
            181 CTTTACCGAC GCACATATTC AGGTGGACGT TACCTACGCC GAGTACTTCG AGATGAGCGT
            241 TCGGCTGGCA GAAGCTATGA GGCGCTATGG GCTGAATACA AACCATCGGA TCGTGGTGTG
            301 CAGCGAGAAT AGCTCGCAGT TCTTCATGCC CGTGTTGGGT GCCCTGTTCA TCGGTGTGGC
            361 TGTGGCCCCA GCTAACGACA TCTACAACGA GCGCGAGCTG CTGAACAGCA TGGGCATCAG
            421 CCAGCCCACC GTCGTATTCG TGAGCAAGAA AGGGCTGCGA AAGGTCCTCA ACGTGCAAAA
            481 GAAGCTACCG ATCATACGAA AGATCATCAT CATGGATAGC AAGACCGACT ACCAGGGCTT
            541 CCAAAGCATG TACACCTTCG TGACTTCCCA TTTGCCACCC AGCTTCAACG AGTACGACTT
            601 CGTGCCCGAG AGCTTCGACC GGGACAAAAC CATCGCCCTG ATCATGAACA GTAGTGGTAG
            661 TACAGGATTA CCCAAGGGCG TAGCCCTACC GCACCGCACC GCTTGTGTCC GATTCAGTCA
            721 TGCCCGCGAC CCCATCTTCG GCTACCAGAA CATCCCCGAC ACCGCTATCC TCAGCGTGGT
            781 GCCATTTCAC CACGGCTTCN NCATGTTCAC CACGCTGGGC TACTTGATCT GCGGCTTTCG
            841 GGTCGTGCTC ATGTACCGCT TCGAGGANGA NCTATTCTTG CGCAGCTTGC AAGANTATAA
            901 GANTNNATCT GCCCTGCTGG TGCCCACNNN ANTTAGCTGC CTCGCTAANA NCNNTCTCNT
            961 CGACNAGTAC NANCTANNCA GCTTGNNCGA GATNGCNAGC NGNGGGGCNN NNNNCANNNA
          1021 NGGAGGNNNN NGAGGNCNNN NNCNAAANNC NTNNNNNNNN NN
          //
          Primer: Akaluc 576-593
          Region: insert mid(Akaluc mid)
          Sequence file: RDB15782_A8B8n.seq check
          >D04498K4_A8B8_1_Akaluc 576-593_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNGNGNGNN NCNNTACCGC NCCGCACCGC TTGTGTCCGA TTCNGTCATG
             61 CCCGCGACCC CATCTTCGGC TACCAGAACA TCCCCGACAC CGCTATCCTC AGCGTGGTGC
            121 CATTTCACCA CGGCTTCGGC ATGTTCACCA CGCTGGGCTA CTTGATCTGC GGCTTTCGGG
            181 TCGTGCTCAT GTACCGCTTC GAGGAGGAGC TATTCTTGCG CAGCTTGCAA GACTATAAGA
            241 TTCAATCTGC CCTGCTGGTG CCCACACTAT TTAGCTGCCT CGCTAAGAGC ACTCTCATCG
            301 ACAAGTACGA CCTAAGCAGC TTGCGCGAGA TCGCCAGCGG CGGGGCGCCG CTCAGCAAGG
            361 AGGTAGGTGA GGCCGTGGCC AAACGCTTCC GCCTACCAGG CATCCGCCAG GGCTATGGCC
            421 TGACAGAAAC AACCAACGCC GTCATGATCA CCCCCGAGGG GGACCGTAAG CCTGGCTCAG
            481 TAGGCAAGGT GGTGCCCTTC TTCGAGGCTA AGGTGGTAGA CTTGGTCACC GGTAAGACAC
            541 TGGGTGTGAA CCAGCGCGGT GAGCTGTGCG TCCGTGGCCC CATGATCATG AGCGGCTACG
            601 TTAACAACCC CGAGGCTACG AACGCTCTCA TCGACAAGGA CGGCTGGCTG CACAGCGGCG
            661 ACATCGCCTA CTGGGACGAG GACGAGCACT TCTTCATCGT GGACCGGCTG AAGAGCCTGA
            721 TCAAATACAA GGGCTACCAG GTAGCCCCAG CCGAACTGGA GGGCATCCTG CTGCAACACC
            781 CCTACATCTT CGACGCCGGA NTCGCCGGCC TGCCCGANGA CGATGCCGGC GANCTGCCCG
            841 CCGCANTCGN CGTGTTGGAA CACGGTAAAA CCATGANCGA NAAANANNTC GNGGACTATG
            901 TGGCCNGCCN GGNTNNNNNC GCCNAGAANC TGCNCGGNGG TGNNGNNNTT GNNGNTNANN
          961 TCCNNANNNG NATCGNNNGN NNNNNNAGNN GCCNGCNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
          original Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1.
          reference Nasu, Y., Unnaturally aglow with a bright inner light. Science 359 (6378): 868-869 (2018). PMID 29472468.
          reference Kuchimaru, T., A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat. Commun. 7: 11856 (2016). PMID 27297211.
          reference Iwano, S., Development of simple firefly luciferin analogs emitting blue, green, red, and near-infrared biological window light. Tetrahedron 69 (19): 3847-3856 (2013).
          reference Iwano, S., Development of simple firefly luciferin analogs emitting blue, green, red, and near-infrared biological window light. Tetrahedron 69 (19): 3847-3856 (2013).

          2022.08.05

          GNP_filter3_RDBDEP_html_220727.pl