Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAAV2 SynTetOff Venus-Akaluc

Expression vector of Akaluc luciferase.

Sequence (full)	RDB15782zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA vector (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. 付加的提供条件： 利用者は研究成果の公表にあたって、pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA の文献 (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017) を引用し、順天堂大学日置寛之先生への謝辞の表明を必要とする。 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15782_A8B8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CSII_CMV5_v2 Sequence file: RDB15782_A8B8a.seq
>D04498K4_A8B8_1_CSII_CMV5_V2_A01_01_ABI08.ab1     1 TGGTGGGAAA TCGGAGCTCG GTACCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC    61 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT   121 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGC TGATCTGCAC   181 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGGGCT ACGGCCTGCA   241 GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC   301 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG   361 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA   421 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA   481 CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGCCA   541 CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG   601 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC TACCAGTCCG CCCTGAGCAA   661 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT   721 CACTCTCGGC ATGGACGAGC TGTACAAGGG ATCCATGGAA GATGCCAAAA ACATTAAGAA   781 GGGCCCAGCG CCGTTCTACC CACTCGAAGA CGGGACCGCC GGCGAGCAGC TGCACAAAGC   841 CATGAAGCGC TACGCCCTGG TGCCCGGCGC CATCGCCTTT ACCGACGCAC ATATTCAGGT   901 GGACGTTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG CTATGAGGCG   961 CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC GAGAATAGCT CGCAGTTCTT  1021 TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGG TGACCCAGGC TAACGACATC 1081 TACACGAGCG CGAGCCTGGC TGGCACCAGC AATGGGGTCA TCAAGTCCAA GGCGAC //
Primer: Akaluc 1215-1243 Sequence file: RDB15782_A8B8b.seq
>D04498K4_A8B8_1_Akaluc 1215-1243_D01_04_ABI08.ab1     1 TCCTGCGGGC GCTCGCCTAC TGGGACGAGG ACGAGCACTT CTTCATCGTG GACCGGCTGA    61 AGAGCCTGAT CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG GGCATCCTGC   121 TGCAACACCC CTACATCTTC GACGCCGGAG TCGCCGGCCT GCCCGACGAC GATGCCGGCG   181 AGCTGCCCGC CGCAGTCGTC GTGTTGGAAC ACGGTAAAAC CATGACCGAG AAAGAGATCG   241 TGGACTATGT GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT GTTGTGTTTG   301 TGGATGAAGT CCCTAGAGGA TCGACCGGCA AGTTAGACGC CCGCAAGATC CGCGAGATTC   361 TCACTAAGGC CAAGAAGGAC GGCAAGATCG CCGTGTAAAC GCGTCTGTGC CTTCTAGTTG   421 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC   481 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC   541 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG   601 GCATGCTGGG GATGCGGTGG GCTCTATGGG CGGCCGCGTC GAGATATCTA GACCCAGCTT   661 TCTTGTACAA AGTGGTACGC GTGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGCCACTC 721 CCTCTCTGCG CGCT //
Primer: Venus-C_F2 Sequence file: RDB15782_A8B8c.seq
>D04498K4_A8B8_1_Venus-C_F2_B01_02_ABI08.ab1     1 TGCATTGGAC GGCGCAGGAA TCACTCTCGG CATGGACGAG CTGTACAAGG GATCCATGGA    61 AGATGCCAAA AACATTAAGA AGGGCCCAGC GCCGTTCTAC CCACTCGAAG ACGGGACCGC   121 CGGCGAGCAG CTGCACAAAG CCATGAAGCG CTACGCCCTG GTGCCCGGCG CCATCGCCTT   181 TACCGACGCA CATATTCAGG TGGACGTTAC CTACGCCGAG TACTTCGAGA TGAGCGTTCG   241 GCTGGCAGAA GCTATGAGGC GCTATGGGCT GAATACAAAC CATCGGATCG TGGTGTGCAG   301 CGAGAATAGC TCGCAGTTCT TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGT   361 GGCCCCAGCT AACGACATCT ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATCAGCCA   421 GCCCACCGTC GTATTCGTGA GCAAGAAAGG GCTGCGAAAG GTCCTCAACG TGCAAAAGAA   481 GCTACCGATC ATACGAAAGA TCATCATCAT GGATAGCAAG ACCGACTACC AGGGCTTCCA   541 AAGCATGTAC ACCTTCGTGA CTTCCCATTT GCCACCCAGC TTCAACGAGT ACGACTTCGT   601 GCCCGAGAGC TTCGACCGGG ACAAAACCAT CGCCCTGATC ATGAACAGTA GTGGTAGTAC   661 AGGATTACCC AAGGGCGTAG CCCTACCGCA CCGCACCGCT TGTGTCCGAT TCAGTCATGC   721 CCGCGACCCC ATCTTCGGCT ACCAGAACAT CCCCGACACC GCTATCCTCA GCGTGGTGCC   781 ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT   841 CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT   901 TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTGCCTC GCTAAGAGCA CTCTCATCGA   961 CAAGTACGAC CTAAAGCAGC TTGCGCGAGA TCGCCCAGCG GGCGGGGGCG CCCGCTCAGC  1021 AAGAGGGTAG GTGAGGGCCG GTGGACCAAC GCTTCCGCCC TACCAGCAAT CCGCCAGGGC  1081 TATGGCCCTG ACAGGAACAC CAACGGTCGG TCCATGATTC ACCCCCCCGG AAGGGGGGGA 1141 ACCGGT //
Primer: Akaluc 576-593 Sequence file: RDB15782_A8B8d.seq
>D04498K4_A8B8_1_Akaluc 576-593_C01_03_ABI08.ab1     1 CAAAAAAACC AGGGGGCGTA GCCCTACCGC ACCGCACCGC TTGTGTCCGA TTCAGTCATG    61 CCCGCGACCC CATCTTCGGC TACCAGAACA TCCCCGACAC CGCTATCCTC AGCGTGGTGC   121 CATTTCACCA CGGCTTCGGC ATGTTCACCA CGCTGGGCTA CTTGATCTGC GGCTTTCGGG   181 TCGTGCTCAT GTACCGCTTC GAGGAGGAGC TATTCTTGCG CAGCTTGCAA GACTATAAGA   241 TTCAATCTGC CCTGCTGGTG CCCACACTAT TTAGCTGCCT CGCTAAGAGC ACTCTCATCG   301 ACAAGTACGA CCTAAGCAGC TTGCGCGAGA TCGCCAGCGG CGGGGCGCCG CTCAGCAAGG   361 AGGTAGGTGA GGCCGTGGCC AAACGCTTCC GCCTACCAGG CATCCGCCAG GGCTATGGCC   421 TGACAGAAAC AACCAACGCC GTCATGATCA CCCCCGAGGG GGACCGTAAG CCTGGCTCAG   481 TAGGCAAGGT GGTGCCCTTC TTCGAGGCTA AGGTGGTAGA CTTGGTCACC GGTAAGACAC   541 TGGGTGTGAA CCAGCGCGGT GAGCTGTGCG TCCGTGGCCC CATGATCATG AGCGGCTACG   601 TTAACAACCC CGAGGCTACG AACGCTCTCA TCGACAAGGA CGGCTGGCTG CACAGCGGCG   661 ACATCGCCTA CTGGGACGAG GACGAGCACT TCTTCATCGT GGACCGGCTG AAGAGCCTGA   721 TCAAATACAA GGGCTACCAG GTAGCCCCAG CCGAACTGGA GGGCATCCTG CTGCAACACC   781 CCTACATCTT CGACGCCGGA GTCGCCGGCC TGCCCGACGA CGATGCCGGC GAGCTGCCCG   841 CCGCAGTCGT CGTGTTGGAA CACGGTAAAA CCATGACCGA GAAAGAGATC GTGGACTATG   901 TGGCCAGCCA GGTTACAACC GCCAAGAAGC TGCGCGGTGG TGTTGTGTTT GTGGATGAAG   961 TCCCTAGAGG GATCGACCGG CAAGTTAGAC GCCCGCAAGG ATCCGCGAGA TTCTCACTAA  1021 AGGCCCAAGA AGGACGGCAG ATCGCCCGTG TAAACGCGGT CTGTGCCTTC TAGCTGCAGT  1081 CATCTATTGA TTGCCCGACC CCGATGGCAT TCGTTGGACC CTGGGAAGGG TGGCCCACAT 1141 C //
Primer: tTA-Ad R Sequence file: RDB15782_A8B8e.seq
>D04498K4_A8B8_1_tTA-AdR_E01_05_ABI08.ab1     1 GATCCGGGGG GCAGGAGGTT TATGGACTTT GCTCTTGTCC AGTCTAGACA TGGTGAATTC    61 GATATCAAGC TTATCGATAC CGTCCGCCGC AGCGCAGATG GTCGCGCCCG TGCCCCCCTA   121 TCTCGCGCCT CGCGTGGTGC GGTCCGGCTG GGCCGGCGGC GGCGCGGACG CGACCAAGGT   181 GGCCGGGAAG GGGAGTTTGC GGGGGACCGG CGAGTGACGT CAGCGCGCCT TCAGTGCTGA   241 GGCGGCGGTG GCGCGCGCCG CCAGGCGGGG GCGAAGGCAC TGTCCGCGGT GCTGAAGCTG   301 GCAGTGCGCA CGCGCCTCGC CGCATCCTGT TTCCCCTCCC CCTCTCTGAT AGGGGATGCG   361 CAATTTGGGG AATGGGGGTT GGGTGCTTGT CCAGTGGGTC GGGGTCGGTC GTCAGGTAGG   421 CACCCCCACC CCGCCTCATC CTGGTCCTAA AACCCACTTG CACTCATACG CAGGGCCCTC   481 TGCAGCTCGA CCGCGCCGCC CCGGGCGGCT CGCTGCTGCC CCCTAGCGGG GGAGGGACGT   541 AATTACATCC CTGGGGGCTT TGGGGGGGCT CGAGTTTACT CCCTATCAGT GATAGAGAAC   601 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGATG TCGAGTTTAC TCCCTATCAG   661 TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT   721 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTATCC CTATCAGTGA TAGAGAACGT   781 ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGGTAGGC GTGTACGGTG   841 GGAGGCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GAATTCGAGC   901 TCGGTACCGC CACCATGGTG AGCAAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC   961 CTGGTCGAGC TGGACGGGCG ACGTAAACGG GCCACAAGTT TCAGCGTGTC CGGGCGAGGG  1021 CGAGGCGATG CCACCTACGG CAAGCTGACC CTGAAGCTGA TCTGCACCAC CGGGCCAGCT  1081 GCCGTGCCCT GACCATCTTC GTGACAACCC TGGGCCTACC GACCCTGCCA GTGCCTTTCG 1141 AACCCCGC //
Primer: tTA-Ad F Sequence file: RDB15782_A8B8f.seq
>D04498K4_A8B8_1_tTA-AdF_F01_06_ABI08.ab1     1 ACATTCTTTT CGAGCTGGAA CTAATCATAT GTGGCCTGGA GAAACAGCTA AAGTGCGAAA    61 GCGGCGGGCC GGCCGACGCC CTTGACGATT TTGACTTAGA CATGCTCCCA GCCGATGCCC   121 TTGACGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT TGACGATTTT GACCTTGACA   181 TGCTCCCCGG GTAACTAAGT AAGGATCTCA GACATGATAA GATACATTGA TGAGTTTGGA   241 CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT   301 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT   361 TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC   421 AAATGTGGTA AGATCCAAAG CCTGCTTTTT TGTACAAACT TGTCACGTGC GGACCGAGCG 481 GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl