RDB15782 - pAAV2 SynTetOff Venus-Akaluc

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icon below as well as clone information before placing your order.

pAAV2 SynTetOff Venus-Akaluc (#RDB15782)

Expression vector of Akaluc luciferase.

Clone info.	Expression vector of Akaluc luciferase.
Comment	This clone was constructed based on the pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA vector (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017). The following 2 commercial compounds have been demonstrated to be well catalyzed by Akaluc in vivo: (1) TokeOni (AkaLumine-HCl), Sigma-Aldrich. Product No.: 808350. (2) AkaLumine-HCl, Wako Pure Chemical Industry. Product No.: 012-26701, 018-26703. Patent application PCT/JP2017/030491 submitted by RIKEN that covers the creation and use of Akaluc.
Vector backbone	pAAV2-DEST(r) (RDB15111) (Plasmid)
Gene/insert name	firefly (Photinus pyralis) luciferase cDNA
Reference of insert sequence	LC320664 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Sequence (full)	RDB15782zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA vector (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA vector (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017) and an acknowledgment to Dr. Hiroyuki Hioki of the Juntendo University are requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. MTAに書く付加的使用条件：利用者は研究成果の公表にあたって、pENTR1A-SV40LpA-tTAad-SYN-insulator-TRE-GFP-BGHpA の文献 (Sohn, J. et al., PLoS One. 12(1): e0169611, 2017) を引用し、順天堂大学日置寛之先生への謝辞の表明を必要とする。本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15782	pAAV2 SynTetOff Venus-Akaluc	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAAV2 SynTetOff Venus-Akaluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15782).

Reference section:

Iwano, S., Sugiyama, M., Hama, H., Watakabe, A., Hasegawa, N., Kuchimaru, T., Tanaka, K.Z., Takahashi, M., Ishida, Y., Hata, J., Shimozono, S., Namiki, K., Fukano, T., Kiyama, M., Okano, H., Kizaka-Kondoh, S., McHugh, T.J., Yamamori, T., Hioki, H., Maki, S., Miyawaki, A., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486. [link to RRC of NBRP]

Miyawaki, A., Iwano, S., Modified luciferase. Patent publication WO 2018/038248 A1. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15782_A8B8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: tTA-Ad F (Pr0541) Region: insert 5'(tTA-Advanced,SV40 pA,attB1),AAV2 ITR Sequence file: RDB15782_A8B8g.seq
>D04498K4_A8B8_1_tTA-AdF_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTAT GTGGCCTGGA NAAACAGCTA AAGTGCGAAA 61 GCGGCGGGCC GGCCGACGCC CTTGACGATT TTGACTTAGA CATGCTCCCA GCCGATGCCC 121 TTGACGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT TGACGATTTT GACCTTGACA 181 TGCTCCCCGG GTAACTAAGT AAGGATCTCA GACATGATAA GATACATTGA TGAGTTTGGA 241 CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT 301 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT 361 TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC 421 AAATGTGGTA AGATCCAAAG CCTGCTTTTT TGTACAAACT TGTCACGTGC GGACCGAGCG 481 GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGN //
Primer: Akaluc 1215-1243 (Pr0540) Region: insert 3'(Akaluc 3',bGH pA),attB2, AAV2 ITR Sequence file: RDB15782_A8B8h.seq
>D04498K4_A8B8_1_Akaluc 1215-1243_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNGCCNNN NGGGNNGANG NNGNNCNCTT CTTCNTCGTG GANCGGCTGA 61 AGAGCCTGAT CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG GGCATCCTGC 121 TGCAACACCC CTACATCTTC GACGCCGGAG TCGCCGGCCT GCCCGACGAC GATGCCGGCG 181 AGCTGCCCGC CGCAGTCGTC GTGTTGGAAC ACGGTAAAAC CATGACCGAG AAAGAGATCG 241 TGGACTATGT GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT GTTGTGTTTG 301 TGGATGAAGT CCCTAGAGGA TCGACCGGCA AGTTAGACGC CCGCAAGATC CGCGAGATTC 361 TCACTAAGGC CAAGAAGGAC GGCAAGATCG CCGTGTAAAC GCGTCTGTGC CTTCTAGTTG 421 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC 481 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC 541 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG 601 GCATGCTGGG GATGCGGTGG GCTCTATGGG CGGCCGCGTC GAGATATCTA GACCCAGCTT 661 TCTTGTACAA AGTGGTACGC GTGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGNNNNNC 721 CCTCTCTGCG CGNN //
Primer: tTA-Ad R (Pr0542) Region: insert mid(SYN pro,insulator,tiht TRE pro) Sequence file: RDB15782_A8B8i.seq
>D04498K4_A8B8_1_tTA-AdR_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNT NNNNNNNTTT GCTCNNNNNN NGTCTAGACN TGGTGAATTC 61 GATATCAAGC TTATCGATAC CGTCCGCCGC AGCGCAGATG GTCGCGCCCG TGCCCCCCTA 121 TCTCGCGCCT CGCGTGGTGC GGTCCGGCTG GGCCGGCGGC GGCGCGGACG CGACCAAGGT 181 GGCCGGGAAG GGGAGTTTGC GGGGGACCGG CGAGTGACGT CAGCGCGCCT TCAGTGCTGA 241 GGCGGCGGTG GCGCGCGCCG CCAGGCGGGG GCGAAGGCAC TGTCCGCGGT GCTGAAGCTG 301 GCAGTGCGCA CGCGCCTCGC CGCATCCTGT TTCCCCTCCC CCTCTCTGAT AGGGGATGCG 361 CAATTTGGGG AATGGGGGTT GGGTGCTTGT CCAGTGGGTC GGGGTCGGTC GTCAGGTAGG 421 CACCCCCACC CCGCCTCATC CTGGTCCTAA AACCCACTTG CACTCATACG CAGGGCCCTC 481 TGCAGCTCGA CCGCGCCGCC CCGGGCGGCT CGCTGCTGCC CCCTAGCGGG GGAGGGACGT 541 AATTACATCC CTGGGGGCTT TGGGGGGGCT CGAGTTTACT CCCTATCAGT GATAGAGAAC 601 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGATG TCGAGTTTAC TCCCTATCAG 661 TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT 721 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTATCC CTATCAGTGA TAGAGAACGT 781 ATGTCGAGTT TACTCCCTAT CAGTGATAGA GANNGTATGT CGAGGTANGC GTGTACGGTG 841 NNNNGCCTAT ATAAGCAGAN CTCGTTTANT GAACCGTCAG ATCGCCNNGA NAANTCNAGC 901 TCGGNACCGC CNCCNTNGNG AGCNANGGNC GANGANCTNN NCNCCGGGGN NGNGCCNNTC 961 CNNNNNNANN TGGNNNGNCN NNGTNAANNG NNCNCAAGNT NNANNNNNNN NNN //
Primer: EGFP-N_R2 (Pr0905) Region: insert mid(Venus 5',tight TRE pro,Insulator,SYN pro) Sequence file: RDB15782_A8B8j.seq
>15782_15782_B2GdF_1_EGFP-N_R2_A07_01_ABI08.ab1 1 ANNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNCAN GCCGNANCCC AGGGTGGTCA 61 CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATCAGCTTC AGGGTCAGCT 121 TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT 181 CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA 241 CCATGGTGGC GGTACCGAGC TCGAATTCTC CAGGCGATCT GACGGTTCAC TAAACGAGCT 301 CTGCTTATAT AGGCCTCCCA CCGTACACGC CTACCTCGAC ATACGTTCTC TATCACTGAT 361 AGGGAGTAAA CTCGACATAC GTTCTCTATC ACTGATAGGG ATAAACTCGA CATACGTTCT 421 CTATCACTGA TAGGGAGTAA ACTCGACATA CGTTCTCTAT CACTGATAGG GAGTAAACTC 481 GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA TCGTTCTCTA TCACTGATAG 541 GGAGTAAACT CGACATACGT TCTCTATCAC TGATAGGGAG TAAACTCGAG CCCCCCCAAA 601 GCCCCCAGGG ATGTAATTAC GTCCCTCCCC CGCTAGGGGG CAGCAGCGAG CCGCCCGGGG 661 CGGCGCGGTC GAGCTGCAGA GGGCCCTGCG TATGAGTGCA AGTGGGTTTT AGGACCAGGA 721 TGANNNGGGG TGGGGGTGCC TACCTGACNA CCGACNNNNA NCCANTGGAC AANNNNNNAN 781 NNNNNNNNN //
Primer: CSII_CMV5_v2 (Pr0255) Region: insert mid(Venus mid) Sequence file: RDB15782_A8B8k.seq
>D04498K4_A8B8_1_CSII_CMV5_V2_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNCNNN NNNNNGCNNC CNTGGTGAGC AAGGGCGAGG NGCTGTTCNC 61 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT 121 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGC TGATCTGCAC 181 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGGGCT ACGGCCTGCA 241 GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC 301 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG 361 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA 421 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA 481 CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGCCA 541 CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG 601 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC TACCAGTCCG CCCTGAGCAA 661 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGNT 721 CACTCTCGGC ATGGACGAGC TGTACAAGGG ATCCATGGAA GATGCCAAAA ACATTAAGAA 781 GGGCCCAGCG CCGTTCTACC CACTCGAAGA NGGNNNCGCC GGCGANCAGC TGCACNAAGC 841 CNTGAAGCGC TACGCCCTGG TGCCCGGCGC CNTCGCCTTT ANCGACGCAC ATATTCANGN 901 GGNCGTTANC TACGCCNANN ACTTCNAGNT NNNCGTTCNG NNGGNAGANN NTANNNNNNN 961 CTNNNGGNTN NNNNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: insert mid(Venus 3',Akaluc 5') Sequence file: RDB15782_A8B8l.seq
>15782_15782_B2GdF_1_mVenus-M_F_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNANNNNN NCNNGGNNNN NTTNNAGATC CGCCNCAACA TCGAGGACGG 61 CGGCGTGCAG CTNNNCNACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT 121 GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA 181 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA 241 CGAGCTGTAC AAGGGATCCA TGGAAGATGC CAAAAACATT AAGAAGGGCC CAGCGCCGTT 301 CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC AAAGCCATGA AGCGCTACGC 361 CCTGGTGCCC GGCGCCATCG CCTTTACCGA CGCACATATT CAGGTGGACG TTACCTACGC 421 CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG AGGCGCTATG GGCTGAATAC 481 AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTCGCAG TTCTTCATGC CCGTGTTGGG 541 TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC ATCTACAACG AGCGCGAGCT 601 GCTGAACAGC ATGGGCATCA GCCNGCCCAC CGTCGTATTC GTGAGCAANA AANGGNTGCG 661 AAANGNCCTC NANNNGCAAA ANAANCNACC NATCNNNCNA ANNNNNATCN TNNTGGANNN 721 NNNGANCNAN NNNCNNNNNN NNNN //
Primer: Venus-C_F2 (Pr0454) Region: insert mid(Akaluc mid) Sequence file: RDB15782_A8B8m.seq
>15782_15782_B2GdF_1_Venus-C_F2_B07_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NGNNNNNTCT CGGNNNGGNN GAGCTGTACA AGGGNNCCAT 61 GGAAGANGCC AAAAACATTA AGAAGGGCCC AGCGCCGTTC TACCCACTCG AAGACGGGAC 121 CGCCGGCGAG CAGCTGCACA AAGCCATGAA GCGCTACGCC CTGGTGCCCG GCGCCATCGC 181 CTTTACCGAC GCACATATTC AGGTGGACGT TACCTACGCC GAGTACTTCG AGATGAGCGT 241 TCGGCTGGCA GAAGCTATGA GGCGCTATGG GCTGAATACA AACCATCGGA TCGTGGTGTG 301 CAGCGAGAAT AGCTCGCAGT TCTTCATGCC CGTGTTGGGT GCCCTGTTCA TCGGTGTGGC 361 TGTGGCCCCA GCTAACGACA TCTACAACGA GCGCGAGCTG CTGAACAGCA TGGGCATCAG 421 CCAGCCCACC GTCGTATTCG TGAGCAAGAA AGGGCTGCGA AAGGTCCTCA ACGTGCAAAA 481 GAAGCTACCG ATCATACGAA AGATCATCAT CATGGATAGC AAGACCGACT ACCAGGGCTT 541 CCAAAGCATG TACACCTTCG TGACTTCCCA TTTGCCACCC AGCTTCAACG AGTACGACTT 601 CGTGCCCGAG AGCTTCGACC GGGACAAAAC CATCGCCCTG ATCATGAACA GTAGTGGTAG 661 TACAGGATTA CCCAAGGGCG TAGCCCTACC GCACCGCACC GCTTGTGTCC GATTCAGTCA 721 TGCCCGCGAC CCCATCTTCG GCTACCAGAA CATCCCCGAC ACCGCTATCC TCAGCGTGGT 781 GCCATTTCAC CACGGCTTCN NCATGTTCAC CACGCTGGGC TACTTGATCT GCGGCTTTCG 841 GGTCGTGCTC ATGTACCGCT TCGAGGANGA NCTATTCTTG CGCAGCTTGC AAGANTATAA 901 GANTNNATCT GCCCTGCTGG TGCCCACNNN ANTTAGCTGC CTCGCTAANA NCNNTCTCNT 961 CGACNAGTAC NANCTANNCA GCTTGNNCGA GATNGCNAGC NGNGGGGCNN NNNNCANNNA 1021 NGGAGGNNNN NGAGGNCNNN NNCNAAANNC NTNNNNNNNN NN //
Primer: Akaluc 576-593 (Pr0539) Region: insert mid(Akaluc mid) Sequence file: RDB15782_A8B8n.seq
>D04498K4_A8B8_1_Akaluc 576-593_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNGNGNGNN NCNNTACCGC NCCGCACCGC TTGTGTCCGA TTCNGTCATG 61 CCCGCGACCC CATCTTCGGC TACCAGAACA TCCCCGACAC CGCTATCCTC AGCGTGGTGC 121 CATTTCACCA CGGCTTCGGC ATGTTCACCA CGCTGGGCTA CTTGATCTGC GGCTTTCGGG 181 TCGTGCTCAT GTACCGCTTC GAGGAGGAGC TATTCTTGCG CAGCTTGCAA GACTATAAGA 241 TTCAATCTGC CCTGCTGGTG CCCACACTAT TTAGCTGCCT CGCTAAGAGC ACTCTCATCG 301 ACAAGTACGA CCTAAGCAGC TTGCGCGAGA TCGCCAGCGG CGGGGCGCCG CTCAGCAAGG 361 AGGTAGGTGA GGCCGTGGCC AAACGCTTCC GCCTACCAGG CATCCGCCAG GGCTATGGCC 421 TGACAGAAAC AACCAACGCC GTCATGATCA CCCCCGAGGG GGACCGTAAG CCTGGCTCAG 481 TAGGCAAGGT GGTGCCCTTC TTCGAGGCTA AGGTGGTAGA CTTGGTCACC GGTAAGACAC 541 TGGGTGTGAA CCAGCGCGGT GAGCTGTGCG TCCGTGGCCC CATGATCATG AGCGGCTACG 601 TTAACAACCC CGAGGCTACG AACGCTCTCA TCGACAAGGA CGGCTGGCTG CACAGCGGCG 661 ACATCGCCTA CTGGGACGAG GACGAGCACT TCTTCATCGT GGACCGGCTG AAGAGCCTGA 721 TCAAATACAA GGGCTACCAG GTAGCCCCAG CCGAACTGGA GGGCATCCTG CTGCAACACC 781 CCTACATCTT CGACGCCGGA NTCGCCGGCC TGCCCGANGA CGATGCCGGC GANCTGCCCG 841 CCGCANTCGN CGTGTTGGAA CACGGTAAAA CCATGANCGA NAAANANNTC GNGGACTATG 901 TGGCCNGCCN GGNTNNNNNC GCCNAGAANC TGCNCGGNGG TGNNGNNNTT GNNGNTNANN 961 TCCNNANNNG NATCGNNNGN NNNNNNAGNN GCCNGCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486. [link to RRC of NBRP]
original	Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1. [link to RRC of NBRP]
reference	Nasu, Y., Unnaturally aglow with a bright inner light. Science 359 (6378): 868-869 (2018). PMID 29472468.
reference	Kuchimaru, T., A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat. Commun. 7: 11856 (2016). PMID 27297211.
reference	Iwano, S., Development of simple firefly luciferin analogs emitting blue, green, red, and near-infrared biological window light. Tetrahedron 69 (19): 3847-3856 (2013).
reference	Iwano, S., Development of simple firefly luciferin analogs emitting blue, green, red, and near-infrared biological window light. Tetrahedron 69 (19): 3847-3856 (2013).

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firefly (Photinus pyralis) luciferase and ortholog

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firefly (Photinus pyralis) luciferase

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