Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Xpa (#RDB15264)

Plasmid clone of a photoswitchable variant of KikGR.

Clone info. Plasmid clone of a photoswitchable variant of Kikume Green-Red fluorescent protein (KikGR), h41pkve6 as Xpa (crystallizable and photo-activatable).
Vector backbone pcs4 (pCS2+ derivative) (Plasmid)
Selectable markers Amp^r
Gene/insert name Favia favus Kikume Green-Red fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui H, Jinno Y, Shoda K, Tomita A, Matsuda M, Yamashita E, Katayama H, Nakagawa A, Miyawaki A. (2015) A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
宮脇敦史博士による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui H, Jinno Y, Shoda K, Tomita A, Matsuda M, Yamashita E, Katayama H, Nakagawa A, Miyawaki A. (2015) A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考 提供条件は英文のまま記載してください。
宮脇敦史博士の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15264 Xpa DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Xpa was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15264).

Reference section:

Tsutsui, H., Jinno, Y., Shoda, K., Tomita, A., Matsuda, M., Yamashita, E., Katayama, H., Nakagawa, A., Miyawaki, A., A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193 (2015). PMID 25773597. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15264_A7H9p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCALNLF1 ()
Region: SV40 p(A),insert,SP6 pro.,CMVIE94 pro.
Sequence file: RDB15264_A7H9a.seq check
>D04498I4-2_A7H9_pAxCALNL_F1_D01_04_ABI08.ab1
    1 CAAGGGTATC CTTTATCATG TCTGGATCTA CGTAATACGA CTCACTATAG TTCTAGAGGC
   61 TCGAGAGGCC TTGAATTCTT ACTTGGCCAG CCTTGGCAGC CCGGAATGAG CTTTAGCATG
  121 CTCATACAGC TTAACCTTGT TGTAATCCTT GTCATGGCTT GTTATCTCCA TACTATGATC
  181 CACGAAGTGA TAGTCTGGCA ACTGGACAAC CTTCTTTGCT TTGTAAGTAG TTCTGAAGTC
  241 ACATCGGTAA TGGCCACCTC CTTGAAGCAA CAGAGCCATG TTTACATCAC CCTTCAGCAC
  301 TCCATCACGC ACATACATTT TCTCAGTGGA TGGCTCCCAT TTGACGGTCT TCCTCTGCAT
  361 AACTGGACCA TTGGCAGGAA AGTTCACACC ATCAAATCGA ATTTCATTGA CAAAACAGTT
  421 GCTGCCGTCT TTCTTCATCG TTATATTGTT TGTGGCGAGG CAAATTCCCC CGTCTTCGTA
  481 ACTCATGCTT CGTTCCCAAG AATAACCCTC AGGAAACGAC TGCTTGAAGT AGTCTACTAT
  541 TTCTTCTGGG TATTCGACAA ATACCCGGTT GCCGTAATGG AATGCTGTTG TCAGGATATC
  601 GAAAGCAAAA GGAAGAGGTC CGCCCTCTAT GACTGTCAGG TCCACATTCT GTATTCCCTC
  661 GAAAGGCTGG CCACTTCCTT TCCCTGTAAT CACGAACTTG TGCCCGTTTA CAGCGCCTTC
  721 CATACGCAGC TCGATCTTCA TTTCTGATGT AATCACACTC ACCATGGTCG GATCCTGCAA
  781 AAAGAACAAG TAGCTTGTAT TCTATAGTGT CACCTAAATC AAGCTTGCTC CGACGTCCCC
  841 AGGCAGAATG GCGGTTCCCT AAACGAGCAT TGCTTATATA GACCTCCCAT TAGGCACGCC
  901 TACCGCCCAT TTACGTCAAT GGGAACGCCC ATTTGCGTCA TTGCCCCTCC CCATTGACGT
  961 CAATGGGGGA TGTACTTGGC AGCCATCGCG GGCCCATTTA CCGGCCATTG AACGTCAATG
 1021 GGGAGTACTG CCCAATGGTA CCCTGGCCGT ACCTTTCCAA TAGTAAATGT AACTGCTAAG
 1081 CTACTAATTA GTAGGATATG ATGGTACTTG CGAGTTGGGG CCAATTTACC GATCCAATTG
1141 A
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tsutsui, H., A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193 (2015). PMID 25773597. [link to RRC of NBRP]
reference Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. [link to RRC of NBRP]