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Xpa (#RDB15264)

Plasmid clone of a photoswitchable variant of KikGR.

Clone info. Plasmid clone of a photoswitchable variant of Kikume Green-Red fluorescent protein (KikGR), h41pkve6 as Xpa (crystallizable and photo-activatable).
Vector backbone pcs4 (pCS2+ derivative) (Plasmid)
Selectable markers Amp^r
Gene/insert name Favia favus Kikume Green-Red fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15264hts01.seq checkAssembled from experimentally sequenced data.
Publication Tsutsui, H., Mol. Cell 58 (1): 186-193 (2015). PMID 25773597. [link to RRC of NBRP]
Test sheet RDB15264_B0Gdp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Tsutsui, H. et al., Mol. Cell 58 (1): 186-193 (2015).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Tsutsui, H. et al., Mol. Cell 58 (1): 186-193 (2015).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15264 Xpa DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The Xpa was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15264).

Reference section:

Tsutsui, H., Jinno, Y., Shoda, K., Tomita, A., Matsuda, M., Yamashita, E., Katayama, H., Nakagawa, A., Miyawaki, A., A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193 (2015). PMID 25773597. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tsutsui, H., A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193 (2015). PMID 25773597. [link to RRC of NBRP]
reference Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. [link to RRC of NBRP]