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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Xpa

Plasmid clone of a photoswitchable variant of KikGR.

Catalog number RDB15264
Resource name Xpa
Clone info. Plasmid clone of a photoswitchable variant of Kikume Green-Red fluorescent protein (KikGR), h41pkve6 as Xpa (crystallizable and photo-activatable).
Vector backbone pcs4 (pCS2+ derivative) (Plasmid)
Selectable markers Amp^r
Gene/insert name Favia favus Kikume Green-Red fluorescent protein cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui H, Jinno Y, Shoda K, Tomita A, Matsuda M, Yamashita E, Katayama H, Nakagawa A, Miyawaki A. (2015) A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Remarks
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15264 Xpa DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Tsutsui, H., A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193 (2015). PMID 25773597.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15264_A7H9p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCALNLF1
Region: SV40 p(A),insert,SP6 pro.,CMVIE94 pro.
Sequence file: RDB15264_A7H9a.seq check
>D04498I4-2_A7H9_pAxCALNL_F1_D01_04_ABI08.ab1
    1 CAAGGGTATC CTTTATCATG TCTGGATCTA CGTAATACGA CTCACTATAG TTCTAGAGGC
   61 TCGAGAGGCC TTGAATTCTT ACTTGGCCAG CCTTGGCAGC CCGGAATGAG CTTTAGCATG
  121 CTCATACAGC TTAACCTTGT TGTAATCCTT GTCATGGCTT GTTATCTCCA TACTATGATC
  181 CACGAAGTGA TAGTCTGGCA ACTGGACAAC CTTCTTTGCT TTGTAAGTAG TTCTGAAGTC
  241 ACATCGGTAA TGGCCACCTC CTTGAAGCAA CAGAGCCATG TTTACATCAC CCTTCAGCAC
  301 TCCATCACGC ACATACATTT TCTCAGTGGA TGGCTCCCAT TTGACGGTCT TCCTCTGCAT
  361 AACTGGACCA TTGGCAGGAA AGTTCACACC ATCAAATCGA ATTTCATTGA CAAAACAGTT
  421 GCTGCCGTCT TTCTTCATCG TTATATTGTT TGTGGCGAGG CAAATTCCCC CGTCTTCGTA
  481 ACTCATGCTT CGTTCCCAAG AATAACCCTC AGGAAACGAC TGCTTGAAGT AGTCTACTAT
  541 TTCTTCTGGG TATTCGACAA ATACCCGGTT GCCGTAATGG AATGCTGTTG TCAGGATATC
  601 GAAAGCAAAA GGAAGAGGTC CGCCCTCTAT GACTGTCAGG TCCACATTCT GTATTCCCTC
  661 GAAAGGCTGG CCACTTCCTT TCCCTGTAAT CACGAACTTG TGCCCGTTTA CAGCGCCTTC
  721 CATACGCAGC TCGATCTTCA TTTCTGATGT AATCACACTC ACCATGGTCG GATCCTGCAA
  781 AAAGAACAAG TAGCTTGTAT TCTATAGTGT CACCTAAATC AAGCTTGCTC CGACGTCCCC
  841 AGGCAGAATG GCGGTTCCCT AAACGAGCAT TGCTTATATA GACCTCCCAT TAGGCACGCC
  901 TACCGCCCAT TTACGTCAAT GGGAACGCCC ATTTGCGTCA TTGCCCCTCC CCATTGACGT
  961 CAATGGGGGA TGTACTTGGC AGCCATCGCG GGCCCATTTA CCGGCCATTG AACGTCAATG
 1021 GGGAGTACTG CCCAATGGTA CCCTGGCCGT ACCTTTCCAA TAGTAAATGT AACTGCTAAG
 1081 CTACTAATTA GTAGGATATG ATGGTACTTG CGAGTTGGGG CCAATTTACC GATCCAATTG
1141 A
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tsutsui, H., A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193 (2015). PMID 25773597.
reference Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765.

2019.12.08

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